MS023628 (gene) Bitter gourd (TR) v1

Overview
NameMS023628
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionsuccinate dehydrogenase assembly factor 1, mitochondrial
Locationscaffold155: 782847 .. 783107 (-)
RNA-Seq ExpressionMS023628
SyntenyMS023628
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGCCGAAGCTTTCGGGGCTGCAAAAGCAGGTGCTCAGTCTCTACAGAGGATTTCTACGAGCGGCGCGCTCCAAATCCGCCGAAGATCGGCGGCAGATGGAATCGCTTGTCGCCGCCGAGTTTCGCCGGAACGCCGACCAAATCGATCGCAAAAACTTCATCTACATCGAGTACCTCCTCCGCCGAGGTAACAAACAGCTGGATCAGCTCAAAAGCCCGGCCACTGTTCGATTGTCGTCGATGAATGTCTCAAACGCCAGA

mRNA sequence

AGGCCGAAGCTTTCGGGGCTGCAAAAGCAGGTGCTCAGTCTCTACAGAGGATTTCTACGAGCGGCGCGCTCCAAATCCGCCGAAGATCGGCGGCAGATGGAATCGCTTGTCGCCGCCGAGTTTCGCCGGAACGCCGACCAAATCGATCGCAAAAACTTCATCTACATCGAGTACCTCCTCCGCCGAGGTAACAAACAGCTGGATCAGCTCAAAAGCCCGGCCACTGTTCGATTGTCGTCGATGAATGTCTCAAACGCCAGA

Coding sequence (CDS)

AGGCCGAAGCTTTCGGGGCTGCAAAAGCAGGTGCTCAGTCTCTACAGAGGATTTCTACGAGCGGCGCGCTCCAAATCCGCCGAAGATCGGCGGCAGATGGAATCGCTTGTCGCCGCCGAGTTTCGCCGGAACGCCGACCAAATCGATCGCAAAAACTTCATCTACATCGAGTACCTCCTCCGCCGAGGTAACAAACAGCTGGATCAGCTCAAAAGCCCGGCCACTGTTCGATTGTCGTCGATGAATGTCTCAAACGCCAGA

Protein sequence

RPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRRGNKQLDQLKSPATVRLSSMNVSNAR
Homology
BLAST of MS023628 vs. NCBI nr
Match: XP_022141880.1 (succinate dehydrogenase assembly factor 1, mitochondrial [Momordica charantia])

HSP 1 Score: 161.8 bits (408), Expect = 2.7e-36
Identity = 86/87 (98.85%), Postives = 86/87 (98.85%), Query Frame = 0

Query: 1  RPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLL 60
          RPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQID KNFIYIEYLL
Sbjct: 4  RPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLL 63

Query: 61 RRGNKQLDQLKSPATVRLSSMNVSNAR 88
          RRGNKQLDQLKSPATVRLSSMNVSNAR
Sbjct: 64 RRGNKQLDQLKSPATVRLSSMNVSNAR 90

BLAST of MS023628 vs. NCBI nr
Match: XP_038999431.1 (succinate dehydrogenase assembly factor 1, mitochondrial-like [Hibiscus syriacus] >XP_038999436.1 succinate dehydrogenase assembly factor 1, mitochondrial-like [Hibiscus syriacus])

HSP 1 Score: 137.1 bits (344), Expect = 7.2e-29
Identity = 69/83 (83.13%), Postives = 80/83 (96.39%), Query Frame = 0

Query: 3  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRR 62
          KLSG+QKQVLSLYRGFLRAARSK AEDRRQ+ES+V+AEFRRN++Q+DRKNF+YIEYLLRR
Sbjct: 8  KLSGMQKQVLSLYRGFLRAARSKPAEDRRQIESIVSAEFRRNSNQVDRKNFLYIEYLLRR 67

Query: 63 GNKQLDQLKSPATVRLSSMNVSN 86
          GNKQLDQLKSP T+ LSS+NVS+
Sbjct: 68 GNKQLDQLKSPDTIGLSSVNVSS 90

BLAST of MS023628 vs. NCBI nr
Match: XP_008459591.1 (PREDICTED: succinate dehydrogenase assembly factor 1, mitochondrial [Cucumis melo])

HSP 1 Score: 136.7 bits (343), Expect = 9.4e-29
Identity = 71/80 (88.75%), Postives = 77/80 (96.25%), Query Frame = 0

Query: 3  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRR 62
          KLSGLQKQVLSLYR FLRAAR+KS EDR+QMES+VAAEFRRNA QIDRKNFIYIEYLLRR
Sbjct: 3  KLSGLQKQVLSLYRAFLRAARTKSVEDRQQMESIVAAEFRRNAKQIDRKNFIYIEYLLRR 62

Query: 63 GNKQLDQLKSPATVRLSSMN 83
          GNKQLDQLKSPATVRLS+++
Sbjct: 63 GNKQLDQLKSPATVRLSTLD 82

BLAST of MS023628 vs. NCBI nr
Match: XP_038890134.1 (succinate dehydrogenase assembly factor 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 136.0 bits (341), Expect = 1.6e-28
Identity = 71/80 (88.75%), Postives = 76/80 (95.00%), Query Frame = 0

Query: 3  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRR 62
          KLSGLQKQVLSLYR FLRAARSKSAEDRRQ+ES+VAAEFRRNA QIDRKNFI+IEYLLRR
Sbjct: 3  KLSGLQKQVLSLYRAFLRAARSKSAEDRRQIESIVAAEFRRNAKQIDRKNFIHIEYLLRR 62

Query: 63 GNKQLDQLKSPATVRLSSMN 83
          GNKQLDQLKSP TVR S++N
Sbjct: 63 GNKQLDQLKSPGTVRFSTIN 82

BLAST of MS023628 vs. NCBI nr
Match: XP_011656085.1 (succinate dehydrogenase assembly factor 1, mitochondrial [Cucumis sativus] >KGN52665.1 hypothetical protein Csa_008140 [Cucumis sativus])

HSP 1 Score: 135.2 bits (339), Expect = 2.7e-28
Identity = 70/80 (87.50%), Postives = 76/80 (95.00%), Query Frame = 0

Query: 3  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRR 62
          KLSGLQKQVLSLYR FLRAAR+KS EDR+QMES+VA EFRRNA QIDRKNFIYIEYLLRR
Sbjct: 3  KLSGLQKQVLSLYRAFLRAARTKSVEDRQQMESIVATEFRRNAKQIDRKNFIYIEYLLRR 62

Query: 63 GNKQLDQLKSPATVRLSSMN 83
          GNKQLDQLKSPATVRLS+++
Sbjct: 63 GNKQLDQLKSPATVRLSTLD 82

BLAST of MS023628 vs. ExPASy Swiss-Prot
Match: B0K036 (Succinate dehydrogenase assembly factor 1, mitochondrial OS=Rattus norvegicus OX=10116 GN=Sdhaf1 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 8.0e-07
Identity = 35/70 (50.00%), Postives = 45/70 (64.29%), Query Frame = 0

Query: 3  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRR 62
          +LS LQ+QVLSLYR  LRA R K   + R     V AEFR++A  + R + + IEYL RR
Sbjct: 3  RLSRLQRQVLSLYRELLRAGRGKPGAEAR-----VRAEFRQHA-SLPRSDVLRIEYLYRR 62

Query: 63 GNKQLDQLKS 73
          G +QL  L+S
Sbjct: 63 GRRQLQLLRS 66

BLAST of MS023628 vs. ExPASy Swiss-Prot
Match: A6NFY7 (Succinate dehydrogenase assembly factor 1, mitochondrial OS=Homo sapiens OX=9606 GN=SDHAF1 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 3.0e-06
Identity = 34/68 (50.00%), Postives = 43/68 (63.24%), Query Frame = 0

Query: 5  SGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRRGN 64
          S LQ+QVLSLYR  LRA R K   + R     V AEFR++A  + R + + IEYL RRG 
Sbjct: 5  SRLQRQVLSLYRDLLRAGRGKPGAEAR-----VRAEFRQHAG-LPRSDVLRIEYLYRRGR 64

Query: 65 KQLDQLKS 73
          +QL  L+S
Sbjct: 65 RQLQLLRS 66

BLAST of MS023628 vs. ExPASy Swiss-Prot
Match: A8PU71 (Succinate dehydrogenase assembly factor 1, mitochondrial OS=Bos taurus OX=9913 GN=SDHAF1 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 5.2e-06
Identity = 34/68 (50.00%), Postives = 43/68 (63.24%), Query Frame = 0

Query: 5  SGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRRGN 64
          S LQ+QVLSLYR  LRA R K   + R     V AEFR++A  + R + + IEYL RRG 
Sbjct: 5  SRLQRQVLSLYRELLRAGRGKPGAEAR-----VRAEFRQHA-CLPRSDVLRIEYLYRRGR 64

Query: 65 KQLDQLKS 73
          +QL  L+S
Sbjct: 65 RQLQMLRS 66

BLAST of MS023628 vs. ExPASy Swiss-Prot
Match: Q9US02 (Succinate dehydrogenase assembly factor 1, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC664.12c PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 6.8e-06
Identity = 29/70 (41.43%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 4  LSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRRG 63
          LSGLQ+QV+  YR  L AA++K      +  + V  EFR+N   I +++F YIE+LLR G
Sbjct: 3  LSGLQRQVIHFYRRCLHAAKAKEQPYNERWMAFVHQEFRKN-QTISKRDFFYIEHLLRVG 62

Query: 64 NKQLDQLKSP 74
           +Q +    P
Sbjct: 63 QRQYEAYSRP 71

BLAST of MS023628 vs. ExPASy Swiss-Prot
Match: Q54HG5 (Succinate dehydrogenase assembly factor 1A, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=sdhaf1A PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 8.8e-06
Identity = 28/70 (40.00%), Postives = 43/70 (61.43%), Query Frame = 0

Query: 5  SGLQKQVLSLYRGFLRAARSKSAED-RRQMESLVAAEFRRNADQIDRKNFIYIEYLLRRG 64
          SGLQK VLSLYR FLR +  K  ++    + + +  +FR  A  I +++   IE LL +G
Sbjct: 9  SGLQKAVLSLYRSFLRVSNKKEMDNPSSSISNYIKTQFRLKATTIQKRDINKIESLLVKG 68

Query: 65 NKQLDQLKSP 74
           +QL+Q++ P
Sbjct: 69 KRQLEQIQDP 78

BLAST of MS023628 vs. ExPASy TrEMBL
Match: A0A6J1CJC1 (succinate dehydrogenase assembly factor 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111012137 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.3e-36
Identity = 86/87 (98.85%), Postives = 86/87 (98.85%), Query Frame = 0

Query: 1  RPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLL 60
          RPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQID KNFIYIEYLL
Sbjct: 4  RPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLL 63

Query: 61 RRGNKQLDQLKSPATVRLSSMNVSNAR 88
          RRGNKQLDQLKSPATVRLSSMNVSNAR
Sbjct: 64 RRGNKQLDQLKSPATVRLSSMNVSNAR 90

BLAST of MS023628 vs. ExPASy TrEMBL
Match: A0A1S3CAJ2 (succinate dehydrogenase assembly factor 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103498675 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.5e-29
Identity = 71/80 (88.75%), Postives = 77/80 (96.25%), Query Frame = 0

Query: 3  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRR 62
          KLSGLQKQVLSLYR FLRAAR+KS EDR+QMES+VAAEFRRNA QIDRKNFIYIEYLLRR
Sbjct: 3  KLSGLQKQVLSLYRAFLRAARTKSVEDRQQMESIVAAEFRRNAKQIDRKNFIYIEYLLRR 62

Query: 63 GNKQLDQLKSPATVRLSSMN 83
          GNKQLDQLKSPATVRLS+++
Sbjct: 63 GNKQLDQLKSPATVRLSTLD 82

BLAST of MS023628 vs. ExPASy TrEMBL
Match: A0A0A0KST3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G649345 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.3e-28
Identity = 70/80 (87.50%), Postives = 76/80 (95.00%), Query Frame = 0

Query: 3  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRR 62
          KLSGLQKQVLSLYR FLRAAR+KS EDR+QMES+VA EFRRNA QIDRKNFIYIEYLLRR
Sbjct: 3  KLSGLQKQVLSLYRAFLRAARTKSVEDRQQMESIVATEFRRNAKQIDRKNFIYIEYLLRR 62

Query: 63 GNKQLDQLKSPATVRLSSMN 83
          GNKQLDQLKSPATVRLS+++
Sbjct: 63 GNKQLDQLKSPATVRLSTLD 82

BLAST of MS023628 vs. ExPASy TrEMBL
Match: A0A7N2LDJ5 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.9e-28
Identity = 68/83 (81.93%), Postives = 77/83 (92.77%), Query Frame = 0

Query: 2  PKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLR 61
          PKLSG+QKQVLSLYRGFLRAARSKSAEDR ++ES+V+ EFRRN+ Q+DRKNF+YIEYLLR
Sbjct: 7  PKLSGMQKQVLSLYRGFLRAARSKSAEDRHKIESIVSGEFRRNSKQVDRKNFLYIEYLLR 66

Query: 62 RGNKQLDQLKSPATVRLSSMNVS 85
          RG KQLDQLKSP TV LSS+NVS
Sbjct: 67 RGQKQLDQLKSPDTVGLSSLNVS 89

BLAST of MS023628 vs. ExPASy TrEMBL
Match: A0A1U8KMI7 (succinate dehydrogenase assembly factor 1, mitochondrial-like OS=Gossypium hirsutum OX=3635 GN=LOC107918589 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.5e-27
Identity = 65/85 (76.47%), Postives = 78/85 (91.76%), Query Frame = 0

Query: 3  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRR 62
          KLSG+Q+QVLSLYRGFLRAARSK  EDRRQ+ES+V+AEFR+N+ Q+DRKNF+YIEYLLRR
Sbjct: 8  KLSGMQRQVLSLYRGFLRAARSKPTEDRRQIESIVSAEFRQNSKQVDRKNFLYIEYLLRR 67

Query: 63 GNKQLDQLKSPATVRLSSMNVSNAR 88
          G KQLDQLKSP T+ LSS NVS+++
Sbjct: 68 GKKQLDQLKSPDTIGLSSCNVSSSK 92

BLAST of MS023628 vs. TAIR 10
Match: AT2G39725.1 (LYR family of Fe/S cluster biogenesis protein )

HSP 1 Score: 113.6 bits (283), Expect = 7.9e-26
Identity = 58/85 (68.24%), Postives = 71/85 (83.53%), Query Frame = 0

Query: 3  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRR 62
          KLSG+QKQVLSLYRGFLRAARS+  EDR+++E +V+ EFR N+ ++DRKNF YIEYLLR 
Sbjct: 5  KLSGMQKQVLSLYRGFLRAARSRPIEDRKRIEMIVSTEFRHNSKEVDRKNFQYIEYLLRL 64

Query: 63 GNKQLDQLKSPATVRLSSMNVSNAR 88
          G KQLDQLKSPA V LSS+ V  ++
Sbjct: 65 GTKQLDQLKSPAIVSLSSIKVVTSK 89

BLAST of MS023628 vs. TAIR 10
Match: AT2G39725.2 (LYR family of Fe/S cluster biogenesis protein )

HSP 1 Score: 113.6 bits (283), Expect = 7.9e-26
Identity = 58/85 (68.24%), Postives = 71/85 (83.53%), Query Frame = 0

Query: 3  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDRKNFIYIEYLLRR 62
          KLSG+QKQVLSLYRGFLRAARS+  EDR+++E +V+ EFR N+ ++DRKNF YIEYLLR 
Sbjct: 5  KLSGMQKQVLSLYRGFLRAARSRPIEDRKRIEMIVSTEFRHNSKEVDRKNFQYIEYLLRL 64

Query: 63 GNKQLDQLKSPATVRLSSMNVSNAR 88
          G KQLDQLKSPA V LSS+ V  ++
Sbjct: 65 GTKQLDQLKSPAIVSLSSIKVVTSK 89

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141880.12.7e-3698.85succinate dehydrogenase assembly factor 1, mitochondrial [Momordica charantia][more]
XP_038999431.17.2e-2983.13succinate dehydrogenase assembly factor 1, mitochondrial-like [Hibiscus syriacus... [more]
XP_008459591.19.4e-2988.75PREDICTED: succinate dehydrogenase assembly factor 1, mitochondrial [Cucumis mel... [more]
XP_038890134.11.6e-2888.75succinate dehydrogenase assembly factor 1, mitochondrial [Benincasa hispida][more]
XP_011656085.12.7e-2887.50succinate dehydrogenase assembly factor 1, mitochondrial [Cucumis sativus] >KGN5... [more]
Match NameE-valueIdentityDescription
B0K0368.0e-0750.00Succinate dehydrogenase assembly factor 1, mitochondrial OS=Rattus norvegicus OX... [more]
A6NFY73.0e-0650.00Succinate dehydrogenase assembly factor 1, mitochondrial OS=Homo sapiens OX=9606... [more]
A8PU715.2e-0650.00Succinate dehydrogenase assembly factor 1, mitochondrial OS=Bos taurus OX=9913 G... [more]
Q9US026.8e-0641.43Succinate dehydrogenase assembly factor 1, mitochondrial OS=Schizosaccharomyces ... [more]
Q54HG58.8e-0640.00Succinate dehydrogenase assembly factor 1A, mitochondrial OS=Dictyostelium disco... [more]
Match NameE-valueIdentityDescription
A0A6J1CJC11.3e-3698.85succinate dehydrogenase assembly factor 1, mitochondrial OS=Momordica charantia ... [more]
A0A1S3CAJ24.5e-2988.75succinate dehydrogenase assembly factor 1, mitochondrial OS=Cucumis melo OX=3656... [more]
A0A0A0KST31.3e-2887.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G649345 PE=4 SV=1[more]
A0A7N2LDJ52.9e-2881.93Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A1U8KMI75.5e-2776.47succinate dehydrogenase assembly factor 1, mitochondrial-like OS=Gossypium hirsu... [more]
Match NameE-valueIdentityDescription
AT2G39725.17.9e-2668.24LYR family of Fe/S cluster biogenesis protein [more]
AT2G39725.27.9e-2668.24LYR family of Fe/S cluster biogenesis protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008011Complex 1 LYR proteinPFAMPF05347Complex1_LYRcoord: 9..69
e-value: 3.6E-13
score: 49.3
NoneNo IPR availablePANTHERPTHR13675:SF1SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIALcoord: 3..82
NoneNo IPR availablePANTHERPTHR13675LYR MOTIF-CONTAINING PROTEIN 2coord: 3..82
NoneNo IPR availableCDDcd20268Complex1_LYR_SDHAF1_LYRM8coord: 5..69
e-value: 2.19786E-24
score: 84.9554

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS023628.1MS023628.1mRNA