MS023627 (gene) Bitter gourd (TR) v1

Overview
NameMS023627
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionTVP38/TMEM64 family membrane protein
Locationscaffold155: 777707 .. 780795 (+)
RNA-Seq ExpressionMS023627
SyntenyMS023627
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTATAATGCGCCCTCTCCGGCCGCCATCTTCAGCCCCACCGCCATGTCTCCACTTCTCTCCCCCTCTCTCCTCCTCCTTCCTCTTCAGCTTCAGACCCAACAACCGATTCCACTTCCTCAAGCCCTGTTCTTCTCTCAAGCAATCCAAGAAGCCCACTCTTCAGAAGGCCCCCACCGGCGCCCCCCAGAGCTTCAAGTGGCTCTTTAGCCCCAAGGGCGACGACGACGGCGAGAAAGGGAATAAGGGTTCGGCGGAGGGAGATGGTGCCGCTTTGGAGGGCGACACTGCGGTTAAGGGCACCCTCTTGGCCGGTGTTTTGCTTGTTGGTGTGATTGGTGGGTTTGCATTTGCGGGATATGTGTACAGGGATCCGATCAATGCGTTCTTGAATCAGTTCTCCACGTTTATTGATGGTAATTTTGTGATGGGTTGTGTGTTTTTTTGGCGAATGGGAGATTGGATTTGTTAATTCTTTGTGGAATGTTCGATTATTTTGGGGGTATGCTCATTGCTGTTTGAATTGGTCTGAATTATGCATTAATTATCGCAATGGGAGGTTGTTTCTTTCTTAAATTCTTGTTGGGAAGTTCTGTTTGCAGATTCTATTTGTTTGACAATCATTTACCATTCTTCCTCCTCCTCCTTTTGTAATCGAAACCTCAATTTGTGTGGAAGTATGATTTTAGGGGTGATTTTGGTATGGGTTTTAAAATATTGAAAATGCTTTTCAATTCATGGTTTTAGAGATTCTAACTTTTAAGTACAAGTACACTAAGAACTTCTTCCAAAAAAAATTTCAGCATGCTTATAGGAAACTGCTTTAGTTGAAAACACTGCTTTCAAGATCATCCCAAACTCACTCTTATAGATTTCTCTTCTATCAATTTTAGAAAAATTTTAACTATGCTACAGAATGCTGGAATAGTGGATTTAGGGGTGATCATTGGTCAGTTGGTGTCAGTTTGTAGCTCAAACCGACGCCAACCACCAAAAATTCGATTTTTGTCTGTCAGAGCTCAACGGTTTTGGAAGGTTCTACAACTCTGATTGGCCGACTACACCAGCGGTCGATCGTTTTTGGCCAAAGCTGATGCAAAAGAACAGAGCTGAAAATAATGATGGTTAAAGTTGAACTTGGCTCTTTTTAAACAGAACCGATTGACTAAATTGGTTATCTGTTCACTATCACTGTATTTTTGTGGAGTAACTTAATCATCTATCTCCATCTGCAGCTATCTACTTTCTAGTTGTTGTTTCCAAAATCTAATAATTGTCTTTTATTCATGTTAAAGCATGTTTGGTACTATGATAAATGTTTCTTCTTTAATTTAGCTACCATTTCTTTTTTTTTTTTAATTATTTAGCATCCATTATCAAAGGAAAGGGATATATTGAATTATGAGGAGCTCTCCATTCCTCTTAAGGATCCCAATTCATGCAATTGATAACATAGAGCTTTTGATGAACAGGATATGGTCCAGCAGGATATGCTCTATTTGTAGCAGTGTATGCAGGATTGGAGGTATACACAGAATTTTTTCTTTATTAAATACGTGTAGGAAATTTGCCTTCCATTATTAAAGTGTTTACTAATTCCAATGAAAGGCTGGCCTTAGTGGTGAGAACTTGGGAATTGGAATGTTTGGAGTTGCTTTCCAAGATCCTAGGTTCAAGCGAGGTAGAAAACTTCGGGTGTGTTTGGTTGGTGATCCAAAAACAGAAATTTGAAAACAAGTAATTTAATGGAAAACAAAGTTGATATCATCACTATTTTTTAAAATGCGTTTAGCAACCGAAAAATATATTTGTATAGTCCATTAAAATACTATTCTTGCCAAGGCCTACAGCATATATGCTGATAGTATGAATATTGTTTTCCTGTCTAGTTGCTCTAGCACATTTTAGTATCCCATATGGAGTTTGCAGAGATACTTTCATGGTGCCTATTTTGTGTTTCAAGTTTTATTACTACCCATTATGAAATTTATGGTATGATTTTTTACCTTTTAGATCCTTGCAATTCCCGCCATTCCGTTAACAATGTCAGCAGGACTACTCTTTGGCTCTGTAATTGGGACGGTTATAGTTTCCATAAGTGGAACGGTGAGTCTATCTTGGTGCTAGTAAAACATAGCATATGCCTGCTAAATTTTAGCTTATCATCTCCAATGATTTCAGGTGGCTGCTAGTGTTGCCTTTTTGATTGCTCGGTATTTTGCTCGTGAGCGTATTCTTAAGCTCGTAGAAGGAAACAAGAAATTCGCTGCCATTGATAAAGCCATTGGGGAAAATGGTTTCAAAGTAGTCACCCTCCTACGTTTGAGCCCCTTGCTCCCATTTTCTCTAGGAAATTATCTATATGGACTCACATCTGTTAAGTTTGTTCCCTATGTATTGGGAAGGTCAGTTCTCATTTCCTCAATGAAAGTAAAAAATTGTATCTCTATCCCGTTTCCATTACTAGAGAAGACGTTCACATCCATAAATTATTTTAGCTTTTCTTTCGTTCTACTTACTTTCCTGTTCTCCTCTCCTCAACGAGTTGTGTCAGTATATACATAATGCTATGAGCGTGTGAGTTTCAGAGGTTGTGGAAAATGTAAACAATTTATTATCTTGAATGCAGTTGGCTTGGGATGCTTCCGGGAACATGGGCTTACGTCAGTGCAGGTGCATTTGGACGAGCGATTATTGTAAGTTTTCAACCCATCAGCCGTGATGTATTTATATTTTCCACAACAACATTTTACCTTGTGAGTTGAGTAGCTTGTGTTTGGTTGACGATGCTCGAGACGTGTACTTTCATCTTTGGTTTTTCCTTTAAAGATGGTCTATGATCTGGATTCTCGGTCGGGTTTACCGTCTTTATTATCGATCCTTTTCCGCATTTCCAACTTTCCAACCAATTCCGGGTTTATTTCTGATGTAATTCTACATGGGGTGTTTGTGTTTCAGCAAGAAGAATCTGAAGTTGGTTTGCTAGGAGGAGGCAATGGTCAGCTGTGGACTCTGGGACTGGGATTGTTGGCCACAGCACTTGCTGCTGCATACGTTACACGATTAGCCAAGGTAATGAATGTGTCT

mRNA sequence

CTCTCTATAATGCGCCCTCTCCGGCCGCCATCTTCAGCCCCACCGCCATGTCTCCACTTCTCTCCCCCTCTCTCCTCCTCCTTCCTCTTCAGCTTCAGACCCAACAACCGATTCCACTTCCTCAAGCCCTGTTCTTCTCTCAAGCAATCCAAGAAGCCCACTCTTCAGAAGGCCCCCACCGGCGCCCCCCAGAGCTTCAAGTGGCTCTTTAGCCCCAAGGGCGACGACGACGGCGAGAAAGGGAATAAGGGTTCGGCGGAGGGAGATGGTGCCGCTTTGGAGGGCGACACTGCGGTTAAGGGCACCCTCTTGGCCGGTGTTTTGCTTGTTGGTGTGATTGGTGGGTTTGCATTTGCGGGATATGTGTACAGGGATCCGATCAATGCGTTCTTGAATCAGTTCTCCACGTTTATTGATGGATATGGTCCAGCAGGATATGCTCTATTTGTAGCAGTGTATGCAGGATTGGAGATCCTTGCAATTCCCGCCATTCCGTTAACAATGTCAGCAGGACTACTCTTTGGCTCTGTAATTGGGACGGTTATAGTTTCCATAAGTGGAACGGTGGCTGCTAGTGTTGCCTTTTTGATTGCTCGGTATTTTGCTCGTGAGCGTATTCTTAAGCTCGTAGAAGGAAACAAGAAATTCGCTGCCATTGATAAAGCCATTGGGGAAAATGGTTTCAAAGTAGTCACCCTCCTACGTTTGAGCCCCTTGCTCCCATTTTCTCTAGGAAATTATCTATATGGACTCACATCTGTTAAGTTTGTTCCCTATGTATTGGGAAGTTGGCTTGGGATGCTTCCGGGAACATGGGCTTACGTCAGTGCAGGTGCATTTGGACGAGCGATTATTCAAGAAGAATCTGAAGTTGGTTTGCTAGGAGGAGGCAATGGTCAGCTGTGGACTCTGGGACTGGGATTGTTGGCCACAGCACTTGCTGCTGCATACGTTACACGATTAGCCAAGGTAATGAATGTGTCT

Coding sequence (CDS)

CTCTCTATAATGCGCCCTCTCCGGCCGCCATCTTCAGCCCCACCGCCATGTCTCCACTTCTCTCCCCCTCTCTCCTCCTCCTTCCTCTTCAGCTTCAGACCCAACAACCGATTCCACTTCCTCAAGCCCTGTTCTTCTCTCAAGCAATCCAAGAAGCCCACTCTTCAGAAGGCCCCCACCGGCGCCCCCCAGAGCTTCAAGTGGCTCTTTAGCCCCAAGGGCGACGACGACGGCGAGAAAGGGAATAAGGGTTCGGCGGAGGGAGATGGTGCCGCTTTGGAGGGCGACACTGCGGTTAAGGGCACCCTCTTGGCCGGTGTTTTGCTTGTTGGTGTGATTGGTGGGTTTGCATTTGCGGGATATGTGTACAGGGATCCGATCAATGCGTTCTTGAATCAGTTCTCCACGTTTATTGATGGATATGGTCCAGCAGGATATGCTCTATTTGTAGCAGTGTATGCAGGATTGGAGATCCTTGCAATTCCCGCCATTCCGTTAACAATGTCAGCAGGACTACTCTTTGGCTCTGTAATTGGGACGGTTATAGTTTCCATAAGTGGAACGGTGGCTGCTAGTGTTGCCTTTTTGATTGCTCGGTATTTTGCTCGTGAGCGTATTCTTAAGCTCGTAGAAGGAAACAAGAAATTCGCTGCCATTGATAAAGCCATTGGGGAAAATGGTTTCAAAGTAGTCACCCTCCTACGTTTGAGCCCCTTGCTCCCATTTTCTCTAGGAAATTATCTATATGGACTCACATCTGTTAAGTTTGTTCCCTATGTATTGGGAAGTTGGCTTGGGATGCTTCCGGGAACATGGGCTTACGTCAGTGCAGGTGCATTTGGACGAGCGATTATTCAAGAAGAATCTGAAGTTGGTTTGCTAGGAGGAGGCAATGGTCAGCTGTGGACTCTGGGACTGGGATTGTTGGCCACAGCACTTGCTGCTGCATACGTTACACGATTAGCCAAGGTAATGAATGTGTCT

Protein sequence

LSIMRPLRPPSSAPPPCLHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGAPQSFKWLFSPKGDDDGEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWTLGLGLLATALAAAYVTRLAKVMNVS
Homology
BLAST of MS023627 vs. NCBI nr
Match: XP_022141879.1 (uncharacterized protein LOC111012135 [Momordica charantia])

HSP 1 Score: 612.8 bits (1579), Expect = 1.7e-171
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0

Query: 4   MRPLRPPSSAPPPCLHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGAP 63
           MRPLRPPSSAPPPCLHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGAP
Sbjct: 1   MRPLRPPSSAPPPCLHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGAP 60

Query: 64  QSFKWLFSPKGDDDGEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGYVY 123
           QSFKWLFSPKGDDDGEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGYVY
Sbjct: 61  QSFKWLFSPKGDDDGEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGYVY 120

Query: 124 RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIV 183
           RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIV
Sbjct: 121 RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIV 180

Query: 184 SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS 243
           SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS
Sbjct: 181 SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS 240

Query: 244 LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT 303
           LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT
Sbjct: 241 LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT 300

Query: 304 LGLGLLATALAAAYVTRLAK 324
           LGLGLLATALAAAYVTRLAK
Sbjct: 301 LGLGLLATALAAAYVTRLAK 320

BLAST of MS023627 vs. NCBI nr
Match: XP_008459587.1 (PREDICTED: TVP38/TMEM64 family membrane protein slr0305 [Cucumis melo] >KAA0039288.1 TVP38/TMEM64 family membrane protein [Cucumis melo var. makuwa] >TYK00473.1 TVP38/TMEM64 family membrane protein [Cucumis melo var. makuwa])

HSP 1 Score: 567.0 bits (1460), Expect = 1.1e-157
Identity = 303/328 (92.38%), Postives = 312/328 (95.12%), Query Frame = 0

Query: 1   LSIMRPLRPPSSAPPPC---LHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQK 60
           + ++  LRPPSS PPPC   L+FSPPLSSSFLFSFRPN RFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRVLLTLRPPSSNPPPCLSSLNFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  APTGAPQSFKWLFSPKGDDD--GEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGG 120
            PT APQSFKWLFSPK +DD  G+KGNKG A+GDGA LE D+AVKGT+LAGVLLVGVIGG
Sbjct: 61  TPTSAPQSFKWLFSPKSNDDDAGDKGNKG-ADGDGAVLEDDSAVKGTILAGVLLVGVIGG 120

Query: 121 FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG 180
           FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG
Sbjct: 121 FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG 180

Query: 181 SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR 240
           SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR
Sbjct: 181 SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR 240

Query: 241 LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG 300
           LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG
Sbjct: 241 LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG 300

Query: 301 GGNGQLWTLGLGLLATALAAAYVTRLAK 324
           GGNGQLWTLGLGLLATALAAAYVTRLAK
Sbjct: 301 GGNGQLWTLGLGLLATALAAAYVTRLAK 327

BLAST of MS023627 vs. NCBI nr
Match: XP_038890133.1 (TVP38/TMEM64 family membrane protein slr0305 [Benincasa hispida])

HSP 1 Score: 562.4 bits (1448), Expect = 2.6e-156
Identity = 302/322 (93.79%), Postives = 305/322 (94.72%), Query Frame = 0

Query: 7   LRPPSSAPPPC---LHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGAP 66
           LRPPSS PPPC   LHFSPPLSSSFLFSFRPN RFHFLKPCSSLKQSKKPTLQK PT AP
Sbjct: 7   LRPPSSNPPPCLSSLHFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQKTPTSAP 66

Query: 67  QSFKWLFSPKGDDD--GEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGY 126
           QSFKWLFSPK +DD  G+KGNKG  +GDG  LE  TAV GTLLAGVLLVGVIGGFAFAGY
Sbjct: 67  QSFKWLFSPKSNDDDAGDKGNKG-VDGDGDVLEDGTAVTGTLLAGVLLVGVIGGFAFAGY 126

Query: 127 VYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTV 186
           VYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTV
Sbjct: 127 VYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTV 186

Query: 187 IVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLP 246
           IVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLP
Sbjct: 187 IVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLP 246

Query: 247 FSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQL 306
           FSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQL
Sbjct: 247 FSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQL 306

Query: 307 WTLGLGLLATALAAAYVTRLAK 324
           WTLGLGLLATALAAAYVTRLAK
Sbjct: 307 WTLGLGLLATALAAAYVTRLAK 327

BLAST of MS023627 vs. NCBI nr
Match: XP_004141545.1 (uncharacterized protein LOC101204460 [Cucumis sativus] >KGN52660.1 hypothetical protein Csa_007947 [Cucumis sativus])

HSP 1 Score: 562.0 bits (1447), Expect = 3.4e-156
Identity = 302/328 (92.07%), Postives = 309/328 (94.21%), Query Frame = 0

Query: 1   LSIMRPLRPPSSAPPPCL---HFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQK 60
           + ++  LRP SS PPPCL   +FSPPL+SSFLFSFRPN RFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  APTGAPQSFKWLFSPKGDDD--GEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGG 120
            PT APQSFKWLFS K DDD  GEKGNKG  +GDGA LE D+AVKGTLLAGVLLVGVIGG
Sbjct: 61  TPTSAPQSFKWLFSAKSDDDDAGEKGNKG-VDGDGAVLEDDSAVKGTLLAGVLLVGVIGG 120

Query: 121 FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG 180
           FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG
Sbjct: 121 FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG 180

Query: 181 SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR 240
           SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR
Sbjct: 181 SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR 240

Query: 241 LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG 300
           LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG
Sbjct: 241 LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG 300

Query: 301 GGNGQLWTLGLGLLATALAAAYVTRLAK 324
           GGNGQLWTLGLGLLATALAAAYVTRLAK
Sbjct: 301 GGNGQLWTLGLGLLATALAAAYVTRLAK 327

BLAST of MS023627 vs. NCBI nr
Match: XP_022973469.1 (uncharacterized protein LOC111472021 isoform X1 [Cucurbita maxima])

HSP 1 Score: 555.1 bits (1429), Expect = 4.2e-154
Identity = 298/322 (92.55%), Postives = 307/322 (95.34%), Query Frame = 0

Query: 11  SSAPPPC---LHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGAPQSFK 70
           S+ PPPC   LHFSPPLSSSFLFSFRPN RFHFLKPCSSLKQSKKPTLQK+PT APQSFK
Sbjct: 12  SNPPPPCLSSLHFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQKSPTTAPQSFK 71

Query: 71  WLFSPKGDD----DGEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGYVY 130
           WLFSPK D+    D ++G+K +AEGD AALE DTAVKGTLLAGVLLVGVIGGFAFAGYVY
Sbjct: 72  WLFSPKSDEDDDGDADEGSK-AAEGDQAALEDDTAVKGTLLAGVLLVGVIGGFAFAGYVY 131

Query: 131 RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIV 190
           RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGT+IV
Sbjct: 132 RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTIIV 191

Query: 191 SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS 250
           SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS
Sbjct: 192 SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS 251

Query: 251 LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT 310
           LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT
Sbjct: 252 LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT 311

Query: 311 LGLGLLATALAAAYVTRLAKVM 326
           LGLGLLATALAAAYVTRLAKV+
Sbjct: 312 LGLGLLATALAAAYVTRLAKVI 332

BLAST of MS023627 vs. ExPASy Swiss-Prot
Match: Q55909 (TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0305 PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.6e-29
Identity = 77/193 (39.90%), Postives = 114/193 (59.07%), Query Frame = 0

Query: 137 FIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFL 196
           +IDG G      F+ +Y    ++ +P   LT+ AG++FG ++G++ V I  T+ A+ AFL
Sbjct: 11  WIDGLGTWAAIAFMLLYTVATVVFLPGSILTLGAGVVFGVILGSIYVFIGATLGATAAFL 70

Query: 197 IARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKF 256
           + RY AR  + K + GN+KF AID+A+G+ G K+V L RLSP+ PF+L NY YG+T+V  
Sbjct: 71  VGRYLARGWVAKKIAGNQKFKAIDEAVGKEGLKIVILTRLSPVFPFNLLNYAYGITNVSL 130

Query: 257 VPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQL-----WTLGL-GLLA 316
             YV+GS LGM+PGT  YV  G+           +  LG    Q      WT+ + G +A
Sbjct: 131 KDYVIGS-LGMIPGTIMYVYIGSLA-------GSLATLGTATNQANPTLQWTIRIVGFIA 190

Query: 317 TALAAAYVTRLAK 324
           T     YVT++A+
Sbjct: 191 TVAVTIYVTKIAR 195

BLAST of MS023627 vs. ExPASy Swiss-Prot
Match: P76219 (TVP38/TMEM64 family membrane protein YdjX OS=Escherichia coli (strain K12) OX=83333 GN=ydjX PE=3 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 1.6e-20
Identity = 56/144 (38.89%), Postives = 87/144 (60.42%), Query Frame = 0

Query: 131 LNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVA 190
           L    T I   G  GY+L++ ++    +L +P   L ++ G++FG ++GT++  I+ T+A
Sbjct: 33  LPHLQTLIRQSGFFGYSLYILLFIIATLLLLPGSILVIAGGIVFGPLLGTLLSLIAATLA 92

Query: 191 ASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYG 250
           +S +FL+AR+  R+ +LK V  +  F AI+K I  NG   + L RL PL P+++ NY YG
Sbjct: 93  SSCSFLLARWLGRDLLLKYVGHSNTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYG 152

Query: 251 LTSVKFVPYVLGSWLGMLPGTWAY 275
           LT++ F PY L S L  LPG   Y
Sbjct: 153 LTTIAFWPYTLISALTTLPGIVIY 176

BLAST of MS023627 vs. ExPASy Swiss-Prot
Match: P9WFS2 (TVP38/TMEM64 family membrane protein MT1538 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1538 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 7.7e-10
Identity = 42/130 (32.31%), Postives = 69/130 (53.08%), Query Frame = 0

Query: 149 FVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILK 208
           F+ V+  + +   P    T++AGLLFGSV+G  I  +  T +A +A L+ R  A    L 
Sbjct: 72  FLLVHTVVTVPPFPRTAFTLAAGLLFGSVVGVFIAVVGSTASAVIAMLLVR--ATGWQLN 131

Query: 209 LVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGML 268
            +   +    +D+ + E G+  +  LRL P++PF+  NY  G + V+ + +   +  G+L
Sbjct: 132 SLVRRRAINRLDERLRERGWLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLAGLL 191

Query: 269 PGTWAYVSAG 279
           PGT A V  G
Sbjct: 192 PGTAAVVILG 199

BLAST of MS023627 vs. ExPASy Swiss-Prot
Match: P9WFS3 (TVP38/TMEM64 family membrane protein Rv1491c OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv1491c PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 7.7e-10
Identity = 42/130 (32.31%), Postives = 69/130 (53.08%), Query Frame = 0

Query: 149 FVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILK 208
           F+ V+  + +   P    T++AGLLFGSV+G  I  +  T +A +A L+ R  A    L 
Sbjct: 72  FLLVHTVVTVPPFPRTAFTLAAGLLFGSVVGVFIAVVGSTASAVIAMLLVR--ATGWQLN 131

Query: 209 LVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGML 268
            +   +    +D+ + E G+  +  LRL P++PF+  NY  G + V+ + +   +  G+L
Sbjct: 132 SLVRRRAINRLDERLRERGWLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLAGLL 191

Query: 269 PGTWAYVSAG 279
           PGT A V  G
Sbjct: 192 PGTAAVVILG 199

BLAST of MS023627 vs. ExPASy Swiss-Prot
Match: P67118 (TVP38/TMEM64 family membrane protein Mb1528c OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB1528C PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 7.7e-10
Identity = 42/130 (32.31%), Postives = 69/130 (53.08%), Query Frame = 0

Query: 149 FVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILK 208
           F+ V+  + +   P    T++AGLLFGSV+G  I  +  T +A +A L+ R  A    L 
Sbjct: 72  FLLVHTVVTVPPFPRTAFTLAAGLLFGSVVGVFIAVVGSTASAVIAMLLVR--ATGWQLN 131

Query: 209 LVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGML 268
            +   +    +D+ + E G+  +  LRL P++PF+  NY  G + V+ + +   +  G+L
Sbjct: 132 SLVRRRAINRLDERLRERGWLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLAGLL 191

Query: 269 PGTWAYVSAG 279
           PGT A V  G
Sbjct: 192 PGTAAVVILG 199

BLAST of MS023627 vs. ExPASy TrEMBL
Match: A0A6J1CK11 (uncharacterized protein LOC111012135 OS=Momordica charantia OX=3673 GN=LOC111012135 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 8.1e-172
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0

Query: 4   MRPLRPPSSAPPPCLHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGAP 63
           MRPLRPPSSAPPPCLHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGAP
Sbjct: 1   MRPLRPPSSAPPPCLHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGAP 60

Query: 64  QSFKWLFSPKGDDDGEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGYVY 123
           QSFKWLFSPKGDDDGEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGYVY
Sbjct: 61  QSFKWLFSPKGDDDGEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGYVY 120

Query: 124 RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIV 183
           RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIV
Sbjct: 121 RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIV 180

Query: 184 SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS 243
           SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS
Sbjct: 181 SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS 240

Query: 244 LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT 303
           LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT
Sbjct: 241 LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT 300

Query: 304 LGLGLLATALAAAYVTRLAK 324
           LGLGLLATALAAAYVTRLAK
Sbjct: 301 LGLGLLATALAAAYVTRLAK 320

BLAST of MS023627 vs. ExPASy TrEMBL
Match: A0A5A7T8S8 (TVP38/TMEM64 family membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00810 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.1e-158
Identity = 303/328 (92.38%), Postives = 312/328 (95.12%), Query Frame = 0

Query: 1   LSIMRPLRPPSSAPPPC---LHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQK 60
           + ++  LRPPSS PPPC   L+FSPPLSSSFLFSFRPN RFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRVLLTLRPPSSNPPPCLSSLNFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  APTGAPQSFKWLFSPKGDDD--GEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGG 120
            PT APQSFKWLFSPK +DD  G+KGNKG A+GDGA LE D+AVKGT+LAGVLLVGVIGG
Sbjct: 61  TPTSAPQSFKWLFSPKSNDDDAGDKGNKG-ADGDGAVLEDDSAVKGTILAGVLLVGVIGG 120

Query: 121 FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG 180
           FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG
Sbjct: 121 FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG 180

Query: 181 SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR 240
           SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR
Sbjct: 181 SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR 240

Query: 241 LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG 300
           LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG
Sbjct: 241 LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG 300

Query: 301 GGNGQLWTLGLGLLATALAAAYVTRLAK 324
           GGNGQLWTLGLGLLATALAAAYVTRLAK
Sbjct: 301 GGNGQLWTLGLGLLATALAAAYVTRLAK 327

BLAST of MS023627 vs. ExPASy TrEMBL
Match: A0A1S3CB16 (TVP38/TMEM64 family membrane protein slr0305 OS=Cucumis melo OX=3656 GN=LOC103498671 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.1e-158
Identity = 303/328 (92.38%), Postives = 312/328 (95.12%), Query Frame = 0

Query: 1   LSIMRPLRPPSSAPPPC---LHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQK 60
           + ++  LRPPSS PPPC   L+FSPPLSSSFLFSFRPN RFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRVLLTLRPPSSNPPPCLSSLNFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  APTGAPQSFKWLFSPKGDDD--GEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGG 120
            PT APQSFKWLFSPK +DD  G+KGNKG A+GDGA LE D+AVKGT+LAGVLLVGVIGG
Sbjct: 61  TPTSAPQSFKWLFSPKSNDDDAGDKGNKG-ADGDGAVLEDDSAVKGTILAGVLLVGVIGG 120

Query: 121 FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG 180
           FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG
Sbjct: 121 FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG 180

Query: 181 SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR 240
           SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR
Sbjct: 181 SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR 240

Query: 241 LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG 300
           LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG
Sbjct: 241 LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG 300

Query: 301 GGNGQLWTLGLGLLATALAAAYVTRLAK 324
           GGNGQLWTLGLGLLATALAAAYVTRLAK
Sbjct: 301 GGNGQLWTLGLGLLATALAAAYVTRLAK 327

BLAST of MS023627 vs. ExPASy TrEMBL
Match: A0A0A0KST0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G649300 PE=4 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.6e-156
Identity = 302/328 (92.07%), Postives = 309/328 (94.21%), Query Frame = 0

Query: 1   LSIMRPLRPPSSAPPPCL---HFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQK 60
           + ++  LRP SS PPPCL   +FSPPL+SSFLFSFRPN RFHFLKPCSSLKQSKKPTLQK
Sbjct: 1   MRVLLTLRPASSNPPPCLSSPNFSPPLASSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQK 60

Query: 61  APTGAPQSFKWLFSPKGDDD--GEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGG 120
            PT APQSFKWLFS K DDD  GEKGNKG  +GDGA LE D+AVKGTLLAGVLLVGVIGG
Sbjct: 61  TPTSAPQSFKWLFSAKSDDDDAGEKGNKG-VDGDGAVLEDDSAVKGTLLAGVLLVGVIGG 120

Query: 121 FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG 180
           FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG
Sbjct: 121 FAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFG 180

Query: 181 SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR 240
           SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR
Sbjct: 181 SVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLR 240

Query: 241 LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG 300
           LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG
Sbjct: 241 LSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLG 300

Query: 301 GGNGQLWTLGLGLLATALAAAYVTRLAK 324
           GGNGQLWTLGLGLLATALAAAYVTRLAK
Sbjct: 301 GGNGQLWTLGLGLLATALAAAYVTRLAK 327

BLAST of MS023627 vs. ExPASy TrEMBL
Match: A0A6J1IEN7 (uncharacterized protein LOC111472021 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472021 PE=4 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.0e-154
Identity = 298/322 (92.55%), Postives = 307/322 (95.34%), Query Frame = 0

Query: 11  SSAPPPC---LHFSPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGAPQSFK 70
           S+ PPPC   LHFSPPLSSSFLFSFRPN RFHFLKPCSSLKQSKKPTLQK+PT APQSFK
Sbjct: 12  SNPPPPCLSSLHFSPPLSSSFLFSFRPNKRFHFLKPCSSLKQSKKPTLQKSPTTAPQSFK 71

Query: 71  WLFSPKGDD----DGEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGYVY 130
           WLFSPK D+    D ++G+K +AEGD AALE DTAVKGTLLAGVLLVGVIGGFAFAGYVY
Sbjct: 72  WLFSPKSDEDDDGDADEGSK-AAEGDQAALEDDTAVKGTLLAGVLLVGVIGGFAFAGYVY 131

Query: 131 RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIV 190
           RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGT+IV
Sbjct: 132 RDPINAFLNQFSTFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTIIV 191

Query: 191 SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS 250
           SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS
Sbjct: 192 SISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFS 251

Query: 251 LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT 310
           LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT
Sbjct: 252 LGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWT 311

Query: 311 LGLGLLATALAAAYVTRLAKVM 326
           LGLGLLATALAAAYVTRLAKV+
Sbjct: 312 LGLGLLATALAAAYVTRLAKVI 332

BLAST of MS023627 vs. TAIR 10
Match: AT1G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 420.2 bits (1079), Expect = 1.5e-117
Identity = 234/307 (76.22%), Postives = 257/307 (83.71%), Query Frame = 0

Query: 21  SPPLSSSFLFSFRPNNRFHFLKPCSSLKQSKKPTLQKAPTGA--PQSFKWLFSPK--GDD 80
           SPP  SS   SFR   RFHFLKPCSSLKQ+KK   Q  P+ A  PQS +W F+ K   D+
Sbjct: 28  SPPPPSS---SFRLYKRFHFLKPCSSLKQTKKKKQQSLPSTAPPPQSLRWFFNSKSTNDE 87

Query: 81  DGEKGNKGSAEGDGAALEGDTAVKGTLLAGVLLVGVIGGFAFAGYVYRDPINAFLNQFST 140
           + E   K  ++ DG + EGD A+KGT+LAGVLL+G +GGFA  GYVYRD IN FL QFST
Sbjct: 88  NDEDDVKSESDDDGGS-EGDAAIKGTILAGVLLIGTVGGFAGVGYVYRDQINTFLTQFST 147

Query: 141 FIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFL 200
           +I+GYG AGYALF+AVYAGLEILAIPA+PLTMSAGLLFG +IGT+IVSISGT+AASVAFL
Sbjct: 148 YIEGYGTAGYALFIAVYAGLEILAIPALPLTMSAGLLFGPLIGTIIVSISGTMAASVAFL 207

Query: 201 IARYFARERILKLVEGNKKFAAIDKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKF 260
           IARYFARERILKLVE NKKF AIDKAIGENGF+VVTLLRLSPLLPFSLGNYLYGLTSVKF
Sbjct: 208 IARYFARERILKLVEDNKKFLAIDKAIGENGFRVVTLLRLSPLLPFSLGNYLYGLTSVKF 267

Query: 261 VPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWTLGLGLLATALAAA 320
           VPYVLGSWLGMLPG+WAYVSAGAFGRAIIQEES VG L GGNGQL TLG+GLL TALA  
Sbjct: 268 VPYVLGSWLGMLPGSWAYVSAGAFGRAIIQEESNVG-LPGGNGQLLTLGVGLLVTALAGT 327

Query: 321 YVTRLAK 324
           YVT LAK
Sbjct: 328 YVTSLAK 329

BLAST of MS023627 vs. TAIR 10
Match: AT1G03260.1 (SNARE associated Golgi protein family )

HSP 1 Score: 104.8 bits (260), Expect = 1.4e-22
Identity = 64/173 (36.99%), Postives = 96/173 (55.49%), Query Frame = 0

Query: 107 VLLVGVIGGFAFAGYVYRDPINAFLNQFSTFI-DGYGPAGYALFVAVYAGLEILAIPAIP 166
           +LLV ++    F       P+   L  F  +I +  GP G       Y  L I+A+PA  
Sbjct: 18  LLLVAIVSAVIFL------PVEQKLKDFLLWIKEDLGPFGPLALALAYIPLTIVAVPASV 77

Query: 167 LTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDKAIGE 226
           LT+  G LFG  +G V  S+  T+ A+ AFL+ R   +  +   ++   KF A+  AI +
Sbjct: 78  LTLGGGYLFGLPVGFVADSLGATLGATAAFLLGRTIGKSYVTSKIKHYPKFQAVSVAIQK 137

Query: 227 NGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAG 279
           +GFK+V LLR+ P+LPF++ NYL  +T V+   Y+L +WLGM+P T+A V  G
Sbjct: 138 SGFKIVLLLRVVPILPFNMLNYLLSVTPVRLGEYMLATWLGMMPITFALVYVG 184

BLAST of MS023627 vs. TAIR 10
Match: AT5G19070.1 (SNARE associated Golgi protein family )

HSP 1 Score: 103.2 bits (256), Expect = 4.0e-22
Identity = 65/179 (36.31%), Postives = 99/179 (55.31%), Query Frame = 0

Query: 101 GTLLAGVLLVGVIGGFAFAGYVYRDPINAFLNQFSTFID-GYGPAGYALFVAVYAGLEIL 160
           G+ L   +L+ ++     A Y    P+   L  F  +++   GP G       Y  L +L
Sbjct: 6   GSALRISVLLILVAAIVLACYFL--PVEKLLKDFLLWVEQDLGPWGPFALAVAYIPLTVL 65

Query: 161 AIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAI 220
           A+PA  LT+  G LFG  IG V  S+  T+ +  AFL+ R   +  ++  ++   +F ++
Sbjct: 66  AVPASVLTLGGGYLFGLPIGFVADSVGATLGSGAAFLLGRTIGKPFVVAKLKDYPQFQSV 125

Query: 221 DKAIGENGFKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSAG 279
             AI ++GFK+  LLRL+PLLPFS+ NYL  +T ++  PY+L SWLGM+P T A V  G
Sbjct: 126 ALAIEKSGFKICLLLRLAPLLPFSMLNYLLSVTPIRLGPYLLSSWLGMMPITLALVYVG 182

BLAST of MS023627 vs. TAIR 10
Match: AT4G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 60.5 bits (145), Expect = 3.0e-09
Identity = 72/252 (28.57%), Postives = 112/252 (44.44%), Query Frame = 0

Query: 79  EKGNKGSAEGDGAALEGDTA---VKGTLLAGVLLVGVIGGFAFAGYVYRDPINAFLNQFS 138
           EKG +   E +    +       VK +LL   L    + G+ + G +  D     L Q+ 
Sbjct: 34  EKGQESEQEKEEEKTDSSRFWFWVKLSLLFAFLAALAVVGYIWIGPLIMDKELIPLIQWE 93

Query: 139 TFIDGYGPAGYALFVAVYAGLEILAIPAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAF 198
                +   G  +F +V A    + +P+ P    AG+ FG   G +++  +  V  S+ +
Sbjct: 94  IRTFTHPVCGLLVFASV-AIFPTILLPSTPSMWIAGMTFGYGYGFLLIISAAAVGVSLPY 153

Query: 199 LIARYFARERILKLVEGNKKFAAIDKAIGENG----FKVVTLLRLSPLLPFSLGNYLYGL 258
            I + F   +I   +E     AA+ +A GE      F +VTL+R+SP  P+ L NY    
Sbjct: 154 FIGQLFC-HKIQGWLERYPDQAAVLRAAGEGNWLHQFLLVTLIRISP-FPYILYNYCSVA 213

Query: 259 TSVKFVPYVLGSWLGMLPGTWAYVSAGAFGRAIIQEESEVGLLGGGNGQLWTLGLGLLAT 318
           T VK+ PY+ GS LGM+P  +  +  G   R  + E S     G    Q+    LG LAT
Sbjct: 214 TRVKYGPYITGSLLGMVPEVFVAIYTGILVRT-LAEASSAEEQGLSVTQVILNILGFLAT 273

Query: 319 ALAAAYVTRLAK 324
                 +T+ AK
Sbjct: 274 VATTILITKYAK 281

BLAST of MS023627 vs. TAIR 10
Match: AT2G02370.1 (SNARE associated Golgi protein family )

HSP 1 Score: 59.7 bits (143), Expect = 5.1e-09
Identity = 60/225 (26.67%), Postives = 110/225 (48.89%), Query Frame = 0

Query: 103 LLAGVLLVGVIGGFAFAGYVYRDPINAFLNQFSTFIDGYGPAGYALFVAVYAGL-EILAI 162
           + A  LL+ ++ G     +V++  +   L   +T    +G    A+ + V   L  +  I
Sbjct: 61  ICAVALLLTLVFGKWGVPFVFQKVLIPILQWEAT---AFGRPMLAIVLVVSLALFPVFLI 120

Query: 163 PAIPLTMSAGLLFGSVIGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFAAIDK 222
           P+ P    AG++FG  +G VI+ +  T+   + +LI   F R+R+ + ++   + AA+ +
Sbjct: 121 PSGPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLMF-RDRLHQWLKRWPRQAAVLR 180

Query: 223 AIGENG----FKVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGTWAYVSA 282
              E      F+VV + R+SP  P+++ NY   +TS++F PY  GS  GM+P  + Y+ +
Sbjct: 181 LAAEGSWFHQFRVVAIFRVSP-FPYTIFNYAIVVTSMRFWPYFFGSIAGMIPEAFIYIYS 240

Query: 283 GAFGRAIIQEESEVGLLGGGNGQLWTLGLGLLATALAAAYVTRLA 323
           G   R     +        G+ +L T+ +     +L  A VT +A
Sbjct: 241 GRLIRTFADVQY-------GHQRLTTVEIVYNVISLVIAVVTTVA 273

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141879.11.7e-171100.00uncharacterized protein LOC111012135 [Momordica charantia][more]
XP_008459587.11.1e-15792.38PREDICTED: TVP38/TMEM64 family membrane protein slr0305 [Cucumis melo] >KAA00392... [more]
XP_038890133.12.6e-15693.79TVP38/TMEM64 family membrane protein slr0305 [Benincasa hispida][more]
XP_004141545.13.4e-15692.07uncharacterized protein LOC101204460 [Cucumis sativus] >KGN52660.1 hypothetical ... [more]
XP_022973469.14.2e-15492.55uncharacterized protein LOC111472021 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q559099.6e-2939.90TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 68... [more]
P762191.6e-2038.89TVP38/TMEM64 family membrane protein YdjX OS=Escherichia coli (strain K12) OX=83... [more]
P9WFS27.7e-1032.31TVP38/TMEM64 family membrane protein MT1538 OS=Mycobacterium tuberculosis (strai... [more]
P9WFS37.7e-1032.31TVP38/TMEM64 family membrane protein Rv1491c OS=Mycobacterium tuberculosis (stra... [more]
P671187.7e-1032.31TVP38/TMEM64 family membrane protein Mb1528c OS=Mycobacterium bovis (strain ATCC... [more]
Match NameE-valueIdentityDescription
A0A6J1CK118.1e-172100.00uncharacterized protein LOC111012135 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A5A7T8S85.1e-15892.38TVP38/TMEM64 family membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CB165.1e-15892.38TVP38/TMEM64 family membrane protein slr0305 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A0A0KST01.6e-15692.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G649300 PE=4 SV=1[more]
A0A6J1IEN72.0e-15492.55uncharacterized protein LOC111472021 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G22850.11.5e-11776.22SNARE associated Golgi protein family [more]
AT1G03260.11.4e-2236.99SNARE associated Golgi protein family [more]
AT5G19070.14.0e-2236.31SNARE associated Golgi protein family [more]
AT4G22850.13.0e-0928.57SNARE associated Golgi protein family [more]
AT2G02370.15.1e-0926.67SNARE associated Golgi protein family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032816SNARE associated Golgi proteinPFAMPF09335SNARE_assoccoord: 161..279
e-value: 1.2E-23
score: 83.8
NoneNo IPR availablePANTHERPTHR46826FAMILY NOT NAMEDcoord: 12..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS023627.1MS023627.1mRNA