MS023618 (gene) Bitter gourd (TR) v1

Overview


Name	MS023618
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Reverse transcriptase
Location	scaffold155: 701804 .. 702007 (-)
RNA-Seq Expression	MS023618
Synteny	MS023618

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCTTTATTCTAGAGAGGTCTATTCATGACAACATAATTATTGGGCATGAATGTTTGCATTATATTATGGGTAAGAAGACCAGTTTGGATGGTTTCGCAGCCTTAAATCTTGACTTGAGCAAGGTGTACGATCATGTGGAATGGGTATTTTTGGAGAAAATCATGGCGAAGTTGGGTTTTGCTGATGGATGGGTGGAGAAAATT

mRNA sequence

GCCTTTATTCTAGAGAGGTCTATTCATGACAACATAATTATTGGGCATGAATGTTTGCATTATATTATGGGTAAGAAGACCAGTTTGGATGGTTTCGCAGCCTTAAATCTTGACTTGAGCAAGGTGTACGATCATGTGGAATGGGTATTTTTGGAGAAAATCATGGCGAAGTTGGGTTTTGCTGATGGATGGGTGGAGAAAATT

Coding sequence (CDS)

GCCTTTATTCTAGAGAGGTCTATTCATGACAACATAATTATTGGGCATGAATGTTTGCATTATATTATGGGTAAGAAGACCAGTTTGGATGGTTTCGCAGCCTTAAATCTTGACTTGAGCAAGGTGTACGATCATGTGGAATGGGTATTTTTGGAGAAAATCATGGCGAAGTTGGGTTTTGCTGATGGATGGGTGGAGAAAATT

Protein sequence

AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGFADGWVEKI

Homology

[Close]

BLAST of MS023618 vs. NCBI nr
Match: XP_022143493.1 (uncharacterized protein LOC111013370 [Momordica charantia])

HSP 1 Score: 94.0 bits (232), Expect = 5.4e-16
Identity = 42/68 (61.76%), Postives = 50/68 (73.53%), Query Frame = 0


Query: 1  AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
          AFIL R+I DNIIIGHECLH+I  +KT   G AAL LDLSK +D VEW ++E++M K GF
Sbjct: 19 AFILNRAITDNIIIGHECLHHIRNQKTGSSGLAALKLDLSKAFDRVEWTYIERLMLKFGF 78

Query: 61 ADGWVEKI 69
          A  WV  I
Sbjct: 79 ARDWVNLI 86

[Close]

BLAST of MS023618 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 90.1 bits (222), Expect = 7.8e-15
Identity = 40/68 (58.82%), Postives = 50/68 (73.53%), Query Frame = 0


Query: 1   AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
           AF+  R+I DN+IIGHECLH I   K+ L G AAL LDLSK +D VEW +LE IM K+GF
Sbjct: 701 AFVPSRAISDNVIIGHECLHTINSCKSGLIGMAALKLDLSKAFDRVEWTYLECIMRKMGF 760

Query: 61  ADGWVEKI 69
            +GW++ I
Sbjct: 761 NEGWIQAI 768

[Close]

BLAST of MS023618 vs. NCBI nr
Match: KAA3470588.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 89.0 bits (219), Expect = 1.7e-14
Identity = 39/68 (57.35%), Postives = 47/68 (69.12%), Query Frame = 0


Query: 1   AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
           AFIL R IHDNI+I HE +HY+   K   +    + LD+SK YDHVEW FLEK+M  LGF
Sbjct: 220 AFILGRMIHDNILITHELMHYLQSSKNGPNKGFVIKLDMSKAYDHVEWNFLEKVMKNLGF 279

Query: 61  ADGWVEKI 69
            D WV+KI
Sbjct: 280 EDAWVDKI 287

[Close]

BLAST of MS023618 vs. NCBI nr
Match: EEC84982.1 (hypothetical protein OsI_32248 [Oryza sativa Indica Group] >EEE62926.1 hypothetical protein OsJ_17731 [Oryza sativa Japonica Group])

HSP 1 Score: 87.4 bits (215), Expect = 5.1e-14
Identity = 36/68 (52.94%), Postives = 52/68 (76.47%), Query Frame = 0


Query: 1   AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
           AF+ +R I DN+++ +E  H++  K+T  +G+AAL LD+SK YD VEW FLEK+M +LGF
Sbjct: 214 AFVPQRLITDNVLLAYEMTHFMQTKRTGREGYAALKLDMSKAYDRVEWSFLEKMMVRLGF 273

Query: 61  ADGWVEKI 69
           A+GWV+ I
Sbjct: 274 AEGWVKLI 281

[Close]

BLAST of MS023618 vs. NCBI nr
Match: XP_040249920.1 (uncharacterized protein LOC109742703 isoform X1 [Aegilops tauschii subsp. strangulata])

HSP 1 Score: 85.1 bits (209), Expect = 2.5e-13
Identity = 35/68 (51.47%), Postives = 50/68 (73.53%), Query Frame = 0


Query: 1   AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
           AF+  R I DNI++ +EC HY+  K+T  DG+AA+ LD+SK YD VEW FLEK+M +LGF
Sbjct: 358 AFVPGRLITDNILLAYECTHYMQNKRTGKDGYAAVKLDMSKAYDKVEWDFLEKMMYQLGF 417

Query: 61  ADGWVEKI 69
            + W++ +
Sbjct: 418 EERWIKLV 425

[Close]

BLAST of MS023618 vs. ExPASy TrEMBL
Match: A0A6J1CQU9 (uncharacterized protein LOC111013370 OS=Momordica charantia OX=3673 GN=LOC111013370 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.6e-16
Identity = 42/68 (61.76%), Postives = 50/68 (73.53%), Query Frame = 0


Query: 1  AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
          AFIL R+I DNIIIGHECLH+I  +KT   G AAL LDLSK +D VEW ++E++M K GF
Sbjct: 19 AFILNRAITDNIIIGHECLHHIRNQKTGSSGLAALKLDLSKAFDRVEWTYIERLMLKFGF 78

Query: 61 ADGWVEKI 69
          A  WV  I
Sbjct: 79 ARDWVNLI 86

[Close]

BLAST of MS023618 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.8e-15
Identity = 40/68 (58.82%), Postives = 50/68 (73.53%), Query Frame = 0


Query: 1   AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
           AF+  R+I DN+IIGHECLH I   K+ L G AAL LDLSK +D VEW +LE IM K+GF
Sbjct: 701 AFVPSRAISDNVIIGHECLHTINSCKSGLIGMAALKLDLSKAFDRVEWTYLECIMRKMGF 760

Query: 61  ADGWVEKI 69
            +GW++ I
Sbjct: 761 NEGWIQAI 768

[Close]

BLAST of MS023618 vs. ExPASy TrEMBL
Match: A0A5B6VNM6 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_016283 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 8.5e-15
Identity = 39/68 (57.35%), Postives = 47/68 (69.12%), Query Frame = 0


Query: 1   AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
           AFIL R IHDNI+I HE +HY+   K   +    + LD+SK YDHVEW FLEK+M  LGF
Sbjct: 220 AFILGRMIHDNILITHELMHYLQSSKNGPNKGFVIKLDMSKAYDHVEWNFLEKVMKNLGF 279

Query: 61  ADGWVEKI 69
            D WV+KI
Sbjct: 280 EDAWVDKI 287

[Close]

BLAST of MS023618 vs. ExPASy TrEMBL
Match: B8BE31 (Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_32248 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.5e-14
Identity = 36/68 (52.94%), Postives = 52/68 (76.47%), Query Frame = 0


Query: 1   AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
           AF+ +R I DN+++ +E  H++  K+T  +G+AAL LD+SK YD VEW FLEK+M +LGF
Sbjct: 214 AFVPQRLITDNVLLAYEMTHFMQTKRTGREGYAALKLDMSKAYDRVEWSFLEKMMVRLGF 273

Query: 61  ADGWVEKI 69
           A+GWV+ I
Sbjct: 274 AEGWVKLI 281

[Close]

BLAST of MS023618 vs. ExPASy TrEMBL
Match: B9FNE2 (Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_17731 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.5e-14
Identity = 36/68 (52.94%), Postives = 52/68 (76.47%), Query Frame = 0


Query: 1   AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
           AF+ +R I DN+++ +E  H++  K+T  +G+AAL LD+SK YD VEW FLEK+M +LGF
Sbjct: 214 AFVPQRLITDNVLLAYEMTHFMQTKRTGREGYAALKLDMSKAYDRVEWSFLEKMMVRLGF 273

Query: 61  ADGWVEKI 69
           A+GWV+ I
Sbjct: 274 AEGWVKLI 281

[Close]

BLAST of MS023618 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 52.0 bits (123), Expect = 2.2e-07
Identity = 25/68 (36.76%), Postives = 37/68 (54.41%), Query Frame = 0


Query: 1  AFILERSIHDNIIIGHECLHYIMGKKTSLDGFAALNLDLSKVYDHVEWVFLEKIMAKLGF 60
          +FI  R   DNI+   E +H  M +K  + G+  L LDL K YD + W +LE  +   GF
Sbjct: 19 SFIPGRVSTDNIVFVQEAVH-SMRRKKGVKGWMLLKLDLEKAYDRIRWDYLEDTLISAGF 78

Query: 61 ADGWVEKI 69
           + W+ +I
Sbjct: 79 PEVWLPEI 85

The following BLAST results are available for this feature:

BLAST of MS023618 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143493.1	5.4e-16	61.76	uncharacterized protein LOC111013370 [Momordica charantia]	[more]
XP_022158377.1	7.8e-15	58.82	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
KAA3470588.1	1.7e-14	57.35	reverse transcriptase [Gossypium australe]	[more]
EEC84982.1	5.1e-14	52.94	hypothetical protein OsI_32248 [Oryza sativa Indica Group] >EEE62926.1 hypotheti...	[more]
XP_040249920.1	2.5e-13	51.47	uncharacterized protein LOC109742703 isoform X1 [Aegilops tauschii subsp. strang...	[more]

BLAST of MS023618 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS023618 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQU9	2.6e-16	61.76	uncharacterized protein LOC111013370 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DX30	3.8e-15	58.82	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A5B6VNM6	8.5e-15	57.35	Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_016283 PE=4 SV=1	[more]
B8BE31	2.5e-14	52.94	Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX...	[more]
B9FNE2	2.5e-14	52.94	Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. japonica ...	[more]

BLAST of MS023618 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20520.1	2.2e-07	36.76	RNA binding;RNA-directed DNA polymerases	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR19446	REVERSE TRANSCRIPTASES	coord: 1..68
None	No IPR available	PANTHER	PTHR19446:SF440	SUBFAMILY NOT NAMED	coord: 1..68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS023618.1	MS023618.1	mRNA