MS023617 (gene) Bitter gourd (TR) v1

Overview
NameMS023617
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRibonuclease H-like superfamily protein
Locationscaffold155: 700277 .. 701038 (-)
RNA-Seq ExpressionMS023617
SyntenyMS023617
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTGGTTTAAATTTTCGAGACTTCGAAGGTTTTAACCAAGCGCTCTTGGCCAAACAGGTGTGGAGGTGTTTGACAGCCCTAGATATCTTGGTTTCTAAACTGCTCCGAGCAAAATACTTTCCTAATTCTACCTGTTTGGAGGCTGAGTTGGGGTTAAGACCTTTATACTTTTGGAAAAGCTTTATTTGGGGAAGAGAGTTGTTGGTTAAAGGCCTCCGTAGGAGAGTGGGGGATGGAAAGTCTATCAGAGCGTTTAGGGATGGTTGGTTGCCTAGGCTGTCTATGTTTAAACCTCTGGTGGTGCCTCCTTTATCTCAGGACCTTACGGTTGCAGACTTTATTCGGCTTGATAGGAGTTGGGACAATAATATGCTTCAGAATTGGTTTTGTCGGGAAGATGTGGATATAATTAGAAGATTACCGCTGGGACTGATGTCGACGGAGGACGGGTGGGTGTGGCACTATGATAAGAAGGGGGTCTTCAATGTCAAGAGTGGATATAAGTTTTTTATGCATCTTAAACTAGAATCTTCATCTTTAAATCAGGAAGCCACTAAAAGATCGTGGAGAAATCTATGGAAACTTAGTATTCCGCCTAAAATTAAGCATTTTATATGGAAAGCGATAAACAATATTGTTCCTACAAGATGCAACCTAATCTCGAAAGGGTTAACTATTGAGGATGTTTGTCCTATGTGTGGTAAGGGTGAAGAGACGATTGAGCATACCTTGTTGACATGCAAGCGGGCACGCGAATTTTGG

mRNA sequence

GGTGGTTTAAATTTTCGAGACTTCGAAGGTTTTAACCAAGCGCTCTTGGCCAAACAGGTGTGGAGGTGTTTGACAGCCCTAGATATCTTGGTTTCTAAACTGCTCCGAGCAAAATACTTTCCTAATTCTACCTGTTTGGAGGCTGAGTTGGGGTTAAGACCTTTATACTTTTGGAAAAGCTTTATTTGGGGAAGAGAGTTGTTGGTTAAAGGCCTCCGTAGGAGAGTGGGGGATGGAAAGTCTATCAGAGCGTTTAGGGATGGTTGGTTGCCTAGGCTGTCTATGTTTAAACCTCTGGTGGTGCCTCCTTTATCTCAGGACCTTACGGTTGCAGACTTTATTCGGCTTGATAGGAGTTGGGACAATAATATGCTTCAGAATTGGTTTTGTCGGGAAGATGTGGATATAATTAGAAGATTACCGCTGGGACTGATGTCGACGGAGGACGGGTGGGTGTGGCACTATGATAAGAAGGGGGTCTTCAATGTCAAGAGTGGATATAAGTTTTTTATGCATCTTAAACTAGAATCTTCATCTTTAAATCAGGAAGCCACTAAAAGATCGTGGAGAAATCTATGGAAACTTAGTATTCCGCCTAAAATTAAGCATTTTATATGGAAAGCGATAAACAATATTGTTCCTACAAGATGCAACCTAATCTCGAAAGGGTTAACTATTGAGGATGTTTGTCCTATGTGTGGTAAGGGTGAAGAGACGATTGAGCATACCTTGTTGACATGCAAGCGGGCACGCGAATTTTGG

Coding sequence (CDS)

GGTGGTTTAAATTTTCGAGACTTCGAAGGTTTTAACCAAGCGCTCTTGGCCAAACAGGTGTGGAGGTGTTTGACAGCCCTAGATATCTTGGTTTCTAAACTGCTCCGAGCAAAATACTTTCCTAATTCTACCTGTTTGGAGGCTGAGTTGGGGTTAAGACCTTTATACTTTTGGAAAAGCTTTATTTGGGGAAGAGAGTTGTTGGTTAAAGGCCTCCGTAGGAGAGTGGGGGATGGAAAGTCTATCAGAGCGTTTAGGGATGGTTGGTTGCCTAGGCTGTCTATGTTTAAACCTCTGGTGGTGCCTCCTTTATCTCAGGACCTTACGGTTGCAGACTTTATTCGGCTTGATAGGAGTTGGGACAATAATATGCTTCAGAATTGGTTTTGTCGGGAAGATGTGGATATAATTAGAAGATTACCGCTGGGACTGATGTCGACGGAGGACGGGTGGGTGTGGCACTATGATAAGAAGGGGGTCTTCAATGTCAAGAGTGGATATAAGTTTTTTATGCATCTTAAACTAGAATCTTCATCTTTAAATCAGGAAGCCACTAAAAGATCGTGGAGAAATCTATGGAAACTTAGTATTCCGCCTAAAATTAAGCATTTTATATGGAAAGCGATAAACAATATTGTTCCTACAAGATGCAACCTAATCTCGAAAGGGTTAACTATTGAGGATGTTTGTCCTATGTGTGGTAAGGGTGAAGAGACGATTGAGCATACCTTGTTGACATGCAAGCGGGCACGCGAATTTTGG

Protein sequence

GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKSFIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSWDNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSLNQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETIEHTLLTCKRAREFW
Homology
BLAST of MS023617 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 278.1 bits (710), Expect = 7.6e-71
Identity = 123/254 (48.43%), Postives = 171/254 (67.32%), Query Frame = 0

Query: 1    GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
            GGLNFRD EGFNQAL+AK VWR L   ++LVSK+L+ KYF +++ L+A    +  YFWK 
Sbjct: 944  GGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKG 1003

Query: 61   FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSW 120
            F+WGR+LLVKGLR RVG+G +I+AF D WLPR + FKPL     + D TVA FI  D +W
Sbjct: 1004 FLWGRDLLVKGLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALDTTVASFITADGNW 1063

Query: 121  DNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSL 180
            D   + + FC ED D+I  +P+   + +D W+WHYDK+G ++V+SGYK +MHLK  ++S 
Sbjct: 1064 DVTSISHSFCNEDRDLILSMPISSYNLQDSWLWHYDKRGNYSVRSGYKLYMHLKCNATSA 1123

Query: 181  NQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETI 240
            +       W ++WKL++P KIK FIW++ +  +PT  NL+ +G+     C +CG   E+I
Sbjct: 1124 STNYRGTQWNSIWKLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELPACTICGDRRESI 1183

Query: 241  EHTLLTCKRAREFW 255
             H    CKRAR+ W
Sbjct: 1184 IHAFFHCKRARQIW 1197

BLAST of MS023617 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 236.9 bits (603), Expect = 1.9e-58
Identity = 111/254 (43.70%), Postives = 153/254 (60.24%), Query Frame = 0

Query: 1    GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
            GGL FR+F  FNQAL+AKQ WR L   + LVS++L+A+YF NS+ L A+ G    Y W+S
Sbjct: 886  GGLGFREFSCFNQALVAKQAWRLLQYPNSLVSRVLQARYFRNSSFLCAKAGANASYIWRS 945

Query: 61   FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSW 120
             +WGR+++ KG+R R+G+GK I  F D WLPR   F+P+    L     VAD I+ D  W
Sbjct: 946  IMWGRQVIKKGMRWRIGNGKKIAIFSDNWLPRPETFRPIFPLSLPVSSVVADLIKADNQW 1005

Query: 121  DNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSL 180
            D   L+  F   D   I ++PL     ED  +WHYDK+G ++VKSGY+  +  K   S+ 
Sbjct: 1006 DEIKLRQHFLDVDTAEILKIPLPAEKAEDEVLWHYDKRGNYSVKSGYQLALRSKFPDSTS 1065

Query: 181  NQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETI 240
              EA+ + W  LW L +P K+K F+W+A NN++P+  NL  + +  E  C  C    ETI
Sbjct: 1066 CTEASHKYWSALWTLELPEKLKIFMWRASNNLLPSAENLWKRKVVEEPTCKRCKLSVETI 1125

Query: 241  EHTLLTCKRAREFW 255
             H LL CK AR+ W
Sbjct: 1126 SHALLECKAARKIW 1139

BLAST of MS023617 vs. NCBI nr
Match: XP_024035599.1 (uncharacterized protein LOC112096407 [Citrus clementina])

HSP 1 Score: 236.5 bits (602), Expect = 2.5e-58
Identity = 104/254 (40.94%), Postives = 158/254 (62.20%), Query Frame = 0

Query: 1   GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
           GGL FRD   FNQALLAKQ WR L   + LV+K+++A+Y+ N+  L A++G  P + W+S
Sbjct: 579 GGLGFRDVSCFNQALLAKQGWRLLQNPESLVAKVIKARYYKNTDFLNAKVGSSPSFIWRS 638

Query: 61  FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSW 120
            +WGR++L KG R R+G+G+ I+     W+PR + FK +V P L     V++ I  ++ W
Sbjct: 639 ILWGRQVLQKGTRWRIGNGEKIQIQASNWIPRPTTFKLIVEPSLPTGAKVSELILPNQQW 698

Query: 121 DNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSL 180
           + +M+   F R D DII+ + L     ED  +WHYD+KG+++VKSGY+  + +K      
Sbjct: 699 NESMINQSFARMDADIIKSILLPRTPQEDEIIWHYDRKGLYSVKSGYQLALKIKFPEPPT 758

Query: 181 NQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETI 240
           +     + W+NLWKL++P KIK F+WKA    +PT  NL  + +  E +C +C +G E +
Sbjct: 759 SSAGASQEWQNLWKLNLPGKIKIFVWKAAKTFLPTAENLWRRKILQEPICTICKEGREDV 818

Query: 241 EHTLLTCKRAREFW 255
            H L+ CK AR+ W
Sbjct: 819 FHALMECKLARKIW 832

BLAST of MS023617 vs. NCBI nr
Match: XP_024037590.1 (uncharacterized protein LOC112097210 [Citrus clementina])

HSP 1 Score: 233.0 bits (593), Expect = 2.8e-57
Identity = 101/254 (39.76%), Postives = 157/254 (61.81%), Query Frame = 0

Query: 1   GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
           GG+ FRD   FNQAL+AKQ WR +     LV+++L+A+YF ++  + A LG +P + W+S
Sbjct: 650 GGMGFRDLSSFNQALVAKQGWRIMQFPSSLVARVLKARYFKHTGFMNAGLGSKPSFVWRS 709

Query: 61  FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSW 120
            +WGR++L KG R R+G+G+++  + + W+PR + FKP+  P +  D TVA+ I   + W
Sbjct: 710 IVWGRQVLHKGARWRIGNGQNVLVYGNNWIPRPTTFKPISAPSMGTDTTVAELIDEKQQW 769

Query: 121 DNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSL 180
             +++   F  ED + I ++PL     ED  +WHYDKKG ++VKSGY+  M +K      
Sbjct: 770 REDLILQHFRPEDAEAIMQIPLPKRPKEDQLIWHYDKKGYYSVKSGYQVAMRIKFPEDPS 829

Query: 181 NQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETI 240
                +  WR +WKL+IP K+K F+W+A ++++PT  NL  K +  E +C  C    ET+
Sbjct: 830 CSNHDQNLWRFIWKLAIPEKVKIFLWRAAHDLLPTAENLWKKKVLQEPMCQSCHCHVETV 889

Query: 241 EHTLLTCKRAREFW 255
            H L+ C RAR+ W
Sbjct: 890 SHALVECNRARKIW 903

BLAST of MS023617 vs. NCBI nr
Match: XP_015382226.1 (uncharacterized protein LOC107175329, partial [Citrus sinensis])

HSP 1 Score: 231.9 bits (590), Expect = 6.2e-57
Identity = 101/254 (39.76%), Postives = 154/254 (60.63%), Query Frame = 0

Query: 1   GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
           GG+ FRDF  FNQAL+AKQ WR +   + L++++L+AKYF  S+ +EA+LG RP + W+S
Sbjct: 461 GGMGFRDFSCFNQALIAKQGWRIIQHPESLMARVLKAKYFNQSSFMEAKLGSRPSFVWRS 520

Query: 61  FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSW 120
            +WGR+++ KGLR ++G G  +  ++  WLPRL  FKP   P L     VAD I  +++W
Sbjct: 521 ILWGRQIMQKGLRWKIGSGNQVCIYKSNWLPRLEAFKPFSPPTLDIQAVVADLIDENQNW 580

Query: 121 DNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSL 180
              +    F +ED  +I R+ L      D ++WHYDK G ++VKSGY+  + LK   S  
Sbjct: 581 KKEICYQHFMKEDAAMIVRIQLPNTPKPDQYLWHYDKYGKYSVKSGYQIALKLKCPDSPC 640

Query: 181 NQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETI 240
           + +++   W  +W   +P KIK F+W+A+ N++PT  NL  + +    +C  CG   E +
Sbjct: 641 SSDSSLSQWNVIWAAELPEKIKIFMWRAVKNLLPTTSNLWRRKIVGSPICRRCGVKNEDV 700

Query: 241 EHTLLTCKRAREFW 255
            H LL CK A++ W
Sbjct: 701 IHALLDCKVAKKVW 714

BLAST of MS023617 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.7e-20
Identity = 48/99 (48.48%), Postives = 65/99 (65.66%), Query Frame = 0

Query: 1   GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
           GGL FRD   FNQALLAKQ +R +     L+S+LLR++YFP+S+ +E  +G RP Y W+S
Sbjct: 55  GGLGFRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRS 114

Query: 61  FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPL 100
            I GRELL +GL R +GDG   + + D W+   +   PL
Sbjct: 115 IIHGRELLSRGLLRTIGDGIHTKVWLDRWIMDETPLPPL 153

BLAST of MS023617 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.5e-16
Identity = 71/266 (26.69%), Postives = 113/266 (42.48%), Query Frame = 0

Query: 1   GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
           GGL  R  +  N+AL++K  WR L   + L + +L+ KY      +     L P   W S
Sbjct: 99  GGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQKKYHVGE--IRDSRWLIPKGSWSS 158

Query: 61  -----FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLV-----VPPLSQDLTV 120
                 I  R+++  G+    GDG+ IR + D W+      KPL+       P   D  V
Sbjct: 159 TWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSG----KPLLELDNGERPTDCDTVV 218

Query: 121 A-DFIRLDRSWDNNMLQNWFCREDVDIIRRLPLGLMS-TEDGWVWHYDKKGVFNVKSGYK 180
           A D     R WD   +  +        +R + L L++   D   W + + G F+V+S Y+
Sbjct: 219 AKDLWIPGRGWDFAKIDPYTTNNTRLELRAVVLDLVTGARDRLSWKFSQDGQFSVRSAYE 278

Query: 181 FFMHLKLESSSLNQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIED 240
                 L    + +      +  LWK+ +P ++K F+W   N  V T      + L+  +
Sbjct: 279 M-----LTVDEVPRPNMASFFNCLWKVRVPERVKTFLWLVGNQAVMTEEERHRRHLSASN 338

Query: 241 VCPMCGKGEETIEHTLLTCKRAREFW 255
           VC +C  G E++ H L  C      W
Sbjct: 339 VCQVCKGGVESMLHVLRDCPAQLGIW 353

BLAST of MS023617 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.7e-71
Identity = 123/254 (48.43%), Postives = 171/254 (67.32%), Query Frame = 0

Query: 1    GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
            GGLNFRD EGFNQAL+AK VWR L   ++LVSK+L+ KYF +++ L+A    +  YFWK 
Sbjct: 944  GGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKG 1003

Query: 61   FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSW 120
            F+WGR+LLVKGLR RVG+G +I+AF D WLPR + FKPL     + D TVA FI  D +W
Sbjct: 1004 FLWGRDLLVKGLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALDTTVASFITADGNW 1063

Query: 121  DNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSL 180
            D   + + FC ED D+I  +P+   + +D W+WHYDK+G ++V+SGYK +MHLK  ++S 
Sbjct: 1064 DVTSISHSFCNEDRDLILSMPISSYNLQDSWLWHYDKRGNYSVRSGYKLYMHLKCNATSA 1123

Query: 181  NQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETI 240
            +       W ++WKL++P KIK FIW++ +  +PT  NL+ +G+     C +CG   E+I
Sbjct: 1124 STNYRGTQWNSIWKLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELPACTICGDRRESI 1183

Query: 241  EHTLLTCKRAREFW 255
             H    CKRAR+ W
Sbjct: 1184 IHAFFHCKRARQIW 1197

BLAST of MS023617 vs. ExPASy TrEMBL
Match: A0A1S8ACU2 (Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 1.1e-59
Identity = 103/254 (40.55%), Postives = 157/254 (61.81%), Query Frame = 0

Query: 1   GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
           GGL FRD   FNQAL+AKQ WR +   + L++K+L+AKYF  +  L+A+LG +P + W+S
Sbjct: 96  GGLGFRDITSFNQALVAKQSWRIIKYPESLMAKILQAKYFKGADFLQAKLGSKPSFVWRS 155

Query: 61  FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSW 120
            IWGR+++V G+R R+G G  ++ ++  W+PR   FKP+  P L  D TVA+ I  ++ W
Sbjct: 156 IIWGRQVIVNGMRWRIGTGDRVKIYKSHWIPRPQAFKPISAPTLGMDCTVAELIDENQKW 215

Query: 121 DNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSL 180
             +++Q  F  ED ++I R+ L +    D  +WHYDKKG ++VKSGY+  M +K  +   
Sbjct: 216 KESLIQQHFNFEDAELISRIQLPISPKPDQILWHYDKKGNYSVKSGYQIAMRIKHPARPS 275

Query: 181 NQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETI 240
           +  +    W  +W L +P KIK F+WKA  N +PT  NL  + +  E +CP C   +E I
Sbjct: 276 SSSSNLGQWNVIWSLELPEKIKIFMWKAARNFLPTSENLWRRKMVQEPICPRCKMKKEDI 335

Query: 241 EHTLLTCKRAREFW 255
            H ++ CK A++ W
Sbjct: 336 NHAIMVCKAAKKMW 349

BLAST of MS023617 vs. ExPASy TrEMBL
Match: A0A803PEK8 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 9.4e-59
Identity = 111/255 (43.53%), Postives = 150/255 (58.82%), Query Frame = 0

Query: 1    GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
            GGL FRD   FNQALLAKQVWRC+   D L S++L+A YFP  + LEA+ G    + W+S
Sbjct: 1103 GGLGFRDLGLFNQALLAKQVWRCIRFPDNLCSQVLKASYFPTRSILEAKSGAHASFVWRS 1162

Query: 61   FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSW 120
             +WG++++  G R RVG+G+++R   D WL R   FK    PPL ++L VAD    D  W
Sbjct: 1163 LMWGKKVIANGYRWRVGNGETVRVLEDPWLSRPVTFKIYDKPPLPENLYVADLKHGDGRW 1222

Query: 121  DNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSL 180
            D N +++ F  ED ++I   P      ED  +WHY K G + VKSGYK    L  E    
Sbjct: 1223 DENFVRSVFNMEDAELILSTPSTGWDLEDKIMWHYSKNGEYTVKSGYKMASSLATEQYQS 1282

Query: 181  NQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMC-GKGEET 240
            + +     W+ LW L IPPKIKHF+WK   N +PT  NL  +G+ ++++C  C G   ET
Sbjct: 1283 DDQLYVDWWKTLWHLKIPPKIKHFVWKLAYNWIPTSANLAKRGVALDNICERCSGHVVET 1342

Query: 241  IEHTLLTCKRAREFW 255
              H L  CKR++E W
Sbjct: 1343 TAHALWECKRSKELW 1357

BLAST of MS023617 vs. ExPASy TrEMBL
Match: A0A803NHG3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.6e-58
Identity = 107/255 (41.96%), Postives = 149/255 (58.43%), Query Frame = 0

Query: 1    GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
            GGL FRD E FNQALLAKQ+WRCL     L SK+L+A YFP+ + L A+ G    + W+S
Sbjct: 1289 GGLGFRDLELFNQALLAKQIWRCLRQPGSLCSKVLKASYFPHKSVLAAKCGAHASFVWRS 1348

Query: 61   FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSW 120
             +WG+E+++KG R RVG+G+ +R   D WLPR + FK    PP  Q L V D       W
Sbjct: 1349 LVWGKEIILKGYRWRVGNGQQVRVLEDPWLPRPTSFKVYDKPPFPQQLCVVDLTHPSGVW 1408

Query: 121  DNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSL 180
            D + ++  F RED ++I RLP      ED  +WHY + G + V+SGY+    ++   ++L
Sbjct: 1409 DESFIRANFNREDAEMILRLPPLHEDLEDKVMWHYSRNGEYTVRSGYRMAAEIRKSEATL 1468

Query: 181  NQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKG-EET 240
              +  K  WR LWKL +PPK+KHF WK  N+ +PT  NL+++ +  +  C  C  G  E 
Sbjct: 1469 EGQLMKDWWRKLWKLKLPPKVKHFAWKLANSWLPTHSNLVTRKVMTDPTCHRCSNGVREN 1528

Query: 241  IEHTLLTCKRAREFW 255
            I H L  C   +  W
Sbjct: 1529 IFHALWGCSANKGIW 1543

BLAST of MS023617 vs. ExPASy TrEMBL
Match: A0A803QQT2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 6.1e-58
Identity = 106/255 (41.57%), Postives = 148/255 (58.04%), Query Frame = 0

Query: 1   GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
           GGL FRD   FNQALLAKQ+WRCL    +L S++L+A YFP    LEA  G    + W+S
Sbjct: 198 GGLGFRDLGMFNQALLAKQIWRCLRHPQLLCSRVLKASYFPRKGVLEAGCGANASFVWRS 257

Query: 61  FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPLVVPPLSQDLTVADFIRLDRSW 120
            +WG++L++KG R RVG+G+S+R   D WLPR   FK    P L  +L V D    D  W
Sbjct: 258 LVWGKKLILKGYRWRVGNGESVRVLEDPWLPRPVTFKVYDQPSLPANLYVTDLKLADGQW 317

Query: 121 DNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFMHLKLESSSL 180
           D   +++ F   DVD+I  +P      ED  +WHY K G ++VKSGY+       E    
Sbjct: 318 DEGFIRSIFNPTDVDLILGIPCSDWDFEDKILWHYSKYGEYSVKSGYRMAASFTTEQHQS 377

Query: 181 NQEATKRSWRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKG-EET 240
           N+ +  + W+ LW+L IPPK+KHF+WK  +N +P   NL  +G+    VC  C    +E+
Sbjct: 378 NEHSIVQWWKKLWRLKIPPKVKHFVWKVAHNWLPANVNLAKRGIASSVVCSRCSSHVDES 437

Query: 241 IEHTLLTCKRAREFW 255
           + H L  CK ++ +W
Sbjct: 438 VAHALWECKASKGYW 452

BLAST of MS023617 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 9.6e-40
Identity = 92/267 (34.46%), Postives = 138/267 (51.69%), Query Frame = 0

Query: 1   GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
           GG+ F+D E FN ALL KQ+WR L+  + L++K+ +++YF  S  L A LG RP + WKS
Sbjct: 54  GGIGFKDIEAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKS 113

Query: 61  FIWGRELLVKGLRRRVGDGKSIRAFRDGWL---PRLSMFKPLVVPP-----LSQDLTVAD 120
               +E+L +G R  VG+G+ I  +R  WL   P  +  +   VPP     +S  L V+D
Sbjct: 114 IHASQEILRQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSD 173

Query: 121 FI-RLDRSWDNNMLQNWFCREDVDIIRRLPLGLMSTEDGWVWHYDKKGVFNVKSGYKFFM 180
            I    R W  ++++  F   +  +I  L  G     D + W Y   G + VKSGY    
Sbjct: 174 LIDESGREWRKDVIEMLFPEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLT 233

Query: 181 HLKLESSSLNQEATKRS----WRNLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIE 240
            + +   S  QE ++ S    ++ +WK    PKI+HF+WK ++N +P    L  + L+ E
Sbjct: 234 QI-INKRSSPQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKE 293

Query: 241 DVCPMCGKGEETIEHTLLTCKRAREFW 255
             C  C   +ET+ H L  C  AR  W
Sbjct: 294 SACIRCPSCKETVNHLLFKCTFARLTW 319

BLAST of MS023617 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 119.8 bits (299), Expect = 3.2e-27
Identity = 67/229 (29.26%), Postives = 111/229 (48.47%), Query Frame = 0

Query: 35  LRAKYFPNSTCLEAELGLRPLYFWKSFIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLS 94
           ++A+YF + + L+A++  +  Y W S + G  LL KG R  +GDG++IR   D  +    
Sbjct: 1   MKARYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIVDSHP 60

Query: 95  MFKPLVVPPLSQDLTVADFIRLDRS---WDNNMLQNWFCREDVDIIRRLPLGLMSTEDGW 154
             +PL      +++T+ +      S   WD++ +  +  + D   I R+ L      D  
Sbjct: 61  P-RPLNTEETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKI 120

Query: 155 VWHYDKKGVFNVKSGYKFFMHLKLESSSLNQEATKRSW------RNLWKLSIPPKIKHFI 214
           +W+Y+  G + V+SGY    H      S N  A             +W L I PK+KHF+
Sbjct: 121 IWNYNTTGEYTVRSGYWLLTH----DPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFL 180

Query: 215 WKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETIEHTLLTCKRAREFW 255
           W+A++  + T   L ++G+ I+  CP C +  E+I H L TC  A   W
Sbjct: 181 WRALSQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAW 224

BLAST of MS023617 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 100.1 bits (248), Expect = 2.6e-21
Identity = 48/99 (48.48%), Postives = 65/99 (65.66%), Query Frame = 0

Query: 1   GGLNFRDFEGFNQALLAKQVWRCLTALDILVSKLLRAKYFPNSTCLEAELGLRPLYFWKS 60
           GGL FRD   FNQALLAKQ +R +     L+S+LLR++YFP+S+ +E  +G RP Y W+S
Sbjct: 55  GGLGFRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRS 114

Query: 61  FIWGRELLVKGLRRRVGDGKSIRAFRDGWLPRLSMFKPL 100
            I GRELL +GL R +GDG   + + D W+   +   PL
Sbjct: 115 IIHGRELLSRGLLRTIGDGIHTKVWLDRWIMDETPLPPL 153

BLAST of MS023617 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.0e-09
Identity = 22/63 (34.92%), Postives = 34/63 (53.97%), Query Frame = 0

Query: 192 LWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETIEHTLLTCKRAR 251
           +WKL + PKIKHF+W+ +   + T   L S+ +  + +C  C   EETI H +  C   +
Sbjct: 37  IWKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYTQ 96

Query: 252 EFW 255
             W
Sbjct: 97  SVW 99

BLAST of MS023617 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 60.1 bits (144), Expect = 3.0e-09
Identity = 27/61 (44.26%), Postives = 37/61 (60.66%), Query Frame = 0

Query: 191 NLWKLSIPPKIKHFIWKAINNIVPTRCNLISKGLTIEDVCPMCGKGEETIEHTLLTCKRA 250
           ++W L I PKIK  IWKA+NN +P    L+S+ ++IE  C  C +  ETI H L  C  A
Sbjct: 8   DIWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRC-RDFETITHILFNCPFA 67

Query: 251 R 252
           +
Sbjct: 68  Q 67

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158377.17.6e-7148.43uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_024950112.11.9e-5843.70uncharacterized protein LOC112496847 [Citrus sinensis][more]
XP_024035599.12.5e-5840.94uncharacterized protein LOC112096407 [Citrus clementina][more]
XP_024037590.12.8e-5739.76uncharacterized protein LOC112097210 [Citrus clementina][more]
XP_015382226.16.2e-5739.76uncharacterized protein LOC107175329, partial [Citrus sinensis][more]
Match NameE-valueIdentityDescription
P932953.7e-2048.48Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
P0C2F62.5e-1626.69Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A6J1DX303.7e-7148.43uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A1S8ACU21.1e-5940.55Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1[more]
A0A803PEK89.4e-5943.53Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803NHG31.6e-5841.96Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QQT26.1e-5841.57Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G29090.19.6e-4034.46Ribonuclease H-like superfamily protein [more]
AT3G09510.13.2e-2729.26Ribonuclease H-like superfamily protein [more]
ATMG00310.12.6e-2148.48RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT2G02650.11.0e-0934.92Ribonuclease H-like superfamily protein [more]
AT3G26855.13.0e-0944.26RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 161..254
e-value: 2.2E-20
score: 73.1
NoneNo IPR availablePANTHERPTHR46736:SF13BNAC03G26750D PROTEINcoord: 65..254
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 65..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS023617.1MS023617.1mRNA