MS023385 (gene) Bitter gourd (TR) v1

BLAST of MS023385 vs. NCBI nr
Match: XP_022154187.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 558/569 (98.07%), Postives = 563/569 (98.95%), Query Frame = 0

Query: 1   MASSNPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHC 60
           MASSNPISLLTFFILFFFLLSSPSHS APVTTVEICQSATSPSFCRSFLPAS A +HSHC
Sbjct: 1   MASSNPISLLTFFILFFFLLSSPSHSTAPVTTVEICQSATSPSFCRSFLPASLASVHSHC 60

Query: 61  RFTLAHALARAHTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNT 120
           RFTLAHALARAH FLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFIS+TLDTVNNT
Sbjct: 61  RFTLAHALARAHAFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISSTLDTVNNT 120

Query: 121 TQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYS 180
           TQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYS
Sbjct: 121 TQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYS 180

Query: 181 LYLSLFKMGWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRR 240
           LYLSLFKMGWVPENMKAPGLPEGKHFRLR GPLNLKMSPENRAYYDRLVHRKRPAAPGRR
Sbjct: 181 LYLSLFKMGWVPENMKAPGLPEGKHFRLRRGPLNLKMSPENRAYYDRLVHRKRPAAPGRR 240

Query: 241 LLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNES 300
           LLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNES
Sbjct: 241 LLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNES 300

Query: 301 VVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGA 360
           VVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGA
Sbjct: 301 VVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGA 360

Query: 361 IKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQN 420
           IKHQAVALRVSAD+TTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVV+QN
Sbjct: 361 IKHQAVALRVSADLTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVIQN 420

Query: 421 CNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEY 480
           CNLY RLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEY
Sbjct: 421 CNLYTRLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEY 480

Query: 481 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATV 540
           SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATV
Sbjct: 481 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATV 540

Query: 541 ASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           ASNFTVSSLLAGDQWLPPTT+PYTGGLIS
Sbjct: 541 ASNFTVSSLLAGDQWLPPTTVPYTGGLIS 569

BLAST of MS023385 vs. NCBI nr
Match: KAG6571391.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 862.4 bits (2227), Expect = 2.1e-246
Identity = 437/568 (76.94%), Postives = 494/568 (86.97%), Query Frame = 0

Query: 2   ASSNPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHCR 61
           +SSNPISLL FF L F   SS   SA P  TVE+CQSATSPSFC+SFLP+SPA +HSHCR
Sbjct: 3   SSSNPISLLLFFSLLFLFTSS---SAVP--TVEVCQSATSPSFCKSFLPSSPASVHSHCR 62

Query: 62  FTLAHALARAHTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNTT 121
           +TL HAL +A TF+ LVD  L L P+L     SALHDC SLA AN++FIS+T+DTVN TT
Sbjct: 63  YTLHHALVQARTFVTLVDLQLTLFPSL-----SALHDCRSLAEANLDFISSTVDTVNTTT 122

Query: 122 QLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYSL 181
             LPFY+ADE++S +SA+ITN DTC++GL   A + + S +G V+KV+ A+S+D+ +YSL
Sbjct: 123 NALPFYQADEMISLLSAVITNVDTCHDGL---AGSGSGSGLGLVDKVVKAISFDRQLYSL 182

Query: 182 YLSLFKMGWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRRL 241
           YLSLFKMGWV +NMKAP LP+  HF   +GPL LKMSP++RAYY+RLVH K+PAA  RRL
Sbjct: 183 YLSLFKMGWVSKNMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLVHGKKPAA--RRL 242

Query: 242 LQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNESV 301
           LQTNY  +GILVNG+VAVD+NG+YD+TTIN AIAAAPN S A NGYFLIFV++G+Y ESV
Sbjct: 243 LQTNYADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESV 302

Query: 302 VVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGAI 361
           VVPK+KKYVLMIGEGNN+TIITGN NVVDGSTTFNSATV+VEGTGFFG NLTITNTAGAI
Sbjct: 303 VVPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAI 362

Query: 362 KHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQNC 421
           KHQAVALRVSADMTTFYSCIFEGYQDTLY H+LRQFYRECD+YGTVDFIFGNAAVVLQNC
Sbjct: 363 KHQAVALRVSADMTTFYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNC 422

Query: 422 NLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEYS 481
           N+YARLPLSGQFNALTAQGRTD NQNTG  IH CTIKPT +LAASPATKSYLGRPWKE+S
Sbjct: 423 NIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPATKSYLGRPWKEFS 482

Query: 482 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATVA 541
           RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEF NSGPGSDTSRR +WAVGVINATVA
Sbjct: 483 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVA 542

Query: 542 SNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           + FTVS +LAGDQWLPPT +PY+GGLIS
Sbjct: 543 NIFTVSEILAGDQWLPPTAVPYSGGLIS 555

BLAST of MS023385 vs. NCBI nr
Match: KAG7011156.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 856.7 bits (2212), Expect = 1.2e-244
Identity = 435/568 (76.58%), Postives = 492/568 (86.62%), Query Frame = 0

Query: 2   ASSNPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHCR 61
           +SSNPISLL FF L F   SS   SA P  TVE+CQSATSPSFC+SFLP+S A +HSHCR
Sbjct: 3   SSSNPISLLLFFSLLFLFTSS---SAVP--TVEVCQSATSPSFCKSFLPSSSASVHSHCR 62

Query: 62  FTLAHALARAHTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNTT 121
           +TL HAL +A TF+ LVD  L L P+L     SALHDC SLA AN++FIS+T+DTVN TT
Sbjct: 63  YTLHHALVQARTFVTLVDLQLTLFPSL-----SALHDCRSLAVANLDFISSTVDTVNTTT 122

Query: 122 QLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYSL 181
             L FY+ADE++S +SA+ITN DTC++GL   A + + S +G V+KV+ A+S+D+ +YSL
Sbjct: 123 NALQFYQADEMISLLSAVITNVDTCHDGL---AGSGSGSGLGLVDKVVKAISFDRQLYSL 182

Query: 182 YLSLFKMGWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRRL 241
           YLSLFKMGWV +NMKAP LP+  HF   +GPL LKMSP++RAYY+RLVH K+PAA  RRL
Sbjct: 183 YLSLFKMGWVSKNMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLVHGKKPAA--RRL 242

Query: 242 LQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNESV 301
           LQTNY  +GILVNG+VAVD+NG+YD+TTIN AIAAAPN S A NGYFLIFV++G+Y ESV
Sbjct: 243 LQTNYADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESV 302

Query: 302 VVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGAI 361
           VVPK+KKYVLMIGEGNN+TIITGN NVVDGSTTFNSATV+VEGTGFFG NLTITNTAGAI
Sbjct: 303 VVPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAI 362

Query: 362 KHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQNC 421
           KHQAVALRVSADMTTFYSCIFEGYQDTLY H+LRQFYRECD+YGTVDFIFGNAAVVLQNC
Sbjct: 363 KHQAVALRVSADMTTFYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNC 422

Query: 422 NLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEYS 481
           N+YARLPLSGQFNALTAQGRTD NQNTG  IH CTIKPT +LAASPATKSYLGRPWKE+S
Sbjct: 423 NIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPATKSYLGRPWKEFS 482

Query: 482 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATVA 541
           RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEF NSGPGSDTSRR +WAVGVINATVA
Sbjct: 483 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVA 542

Query: 542 SNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           + FTVS +LAGDQWLPPT +PY+GGLIS
Sbjct: 543 NIFTVSEILAGDQWLPPTAVPYSGGLIS 555

BLAST of MS023385 vs. NCBI nr
Match: XP_022928019.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 855.9 bits (2210), Expect = 2.0e-244
Identity = 434/568 (76.41%), Postives = 490/568 (86.27%), Query Frame = 0

Query: 2   ASSNPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHCR 61
           +SSNPISLL FF L F   SS   SA P  TVE+CQSATSPSFC+SFLP+SPA +HSHCR
Sbjct: 3   SSSNPISLLLFFSLLFLFTSS---SAVP--TVEVCQSATSPSFCKSFLPSSPASVHSHCR 62

Query: 62  FTLAHALARAHTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNTT 121
           +TL HAL +A TF+ LVD  L L P+L     SALHDC SLA AN++FIS+T+DTVN TT
Sbjct: 63  YTLHHALVQARTFVTLVDLQLTLFPSL-----SALHDCRSLAVANLDFISSTVDTVNTTT 122

Query: 122 QLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYSL 181
             LPFY+ADE++S +SA+ITN DTC++GL  +        +G V+KV+ A+S+D+ +YSL
Sbjct: 123 NALPFYQADEMISLLSAVITNIDTCHDGLAGSGLG-----LGFVDKVVKAISFDRQLYSL 182

Query: 182 YLSLFKMGWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRRL 241
           YLSLFKMGWV +NMKAP LP+  HF    GPL LKMSP++RAYY+RLVH K+PAA  RRL
Sbjct: 183 YLSLFKMGWVSKNMKAPKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVHGKKPAA--RRL 242

Query: 242 LQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNESV 301
           LQTNY  +GILVNG+VAVD+NG+YD+TTIN AIAAAPN S A NGYFLIFV++G+Y ESV
Sbjct: 243 LQTNYADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESV 302

Query: 302 VVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGAI 361
           VVPK+KKYVLMIGEGNN+TIITGN NVVDGSTTFNSATV+VEGTGFFG NLTITNTAGAI
Sbjct: 303 VVPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAI 362

Query: 362 KHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQNC 421
           KHQAVALRVSADMTTFYSCIFEGYQDTLYAH+LRQFYRECD+YGTVDFIFGNAAVVLQNC
Sbjct: 363 KHQAVALRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNC 422

Query: 422 NLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEYS 481
           N+YARLPLSGQFNALTAQGRTD NQNTG  IH CTIKPT +LAASP TKSYLGRPWKE+S
Sbjct: 423 NIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFS 482

Query: 482 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATVA 541
           RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEF NSGPGSDTS+R +WAVGVINATVA
Sbjct: 483 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVA 542

Query: 542 SNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           + FTVS +LAGDQWLPPT +PY+GGLIS
Sbjct: 543 NIFTVSEILAGDQWLPPTAVPYSGGLIS 553

BLAST of MS023385 vs. NCBI nr
Match: XP_023513316.1 (probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 854.4 bits (2206), Expect = 5.7e-244
Identity = 432/569 (75.92%), Postives = 494/569 (86.82%), Query Frame = 0

Query: 1   MASSNPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHC 60
           MASSN   L+ F +LF FL +S S     V+TVE+CQSATSPSFC+SFLP+SPA +HSHC
Sbjct: 1   MASSN---LIFFALLFLFLFTSSS----AVSTVEVCQSATSPSFCKSFLPSSPASVHSHC 60

Query: 61  RFTLAHALARAHTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNT 120
           R+TL HAL +A TF+ LVD  L L P+L     SALHDC SLA  N++FIS+T+DTVN T
Sbjct: 61  RYTLHHALVQARTFVTLVDLQLTLFPSL-----SALHDCRSLAVTNLDFISSTVDTVNTT 120

Query: 121 TQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYS 180
           T  LPFY+ADE++S +SA+ITN DTC++GL   A + +SS +G V+KV+ A+S+D+ +YS
Sbjct: 121 TNALPFYQADEMISLLSAVITNVDTCHDGL---AGSGSSSGLGLVDKVVKAISFDRQLYS 180

Query: 181 LYLSLFKMGWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRR 240
           LYLSLFKMGWVP+NMKAP LP+  HF   +GPL LKMSP++RAYY+RLV  K+PAA  RR
Sbjct: 181 LYLSLFKMGWVPKNMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLVRGKKPAA--RR 240

Query: 241 LLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNES 300
           LLQTNY  +GILVNG+VAVD+NG+YD+TTIN AIAAAP+ S A NGYFLIFV++G+Y ES
Sbjct: 241 LLQTNYADDGILVNGVVAVDQNGAYDFTTINDAIAAAPDKSTAANGYFLIFVTAGIYTES 300

Query: 301 VVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGA 360
           VVVPK+K+YVLMIGEGNN+TIITGN NVVDGSTTFNSATV+VEGTGFFG NLTITNTAGA
Sbjct: 301 VVVPKSKRYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGA 360

Query: 361 IKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQN 420
           IKHQAVALRVSADMTTFYSCIFEGYQDTLY H+LRQFYRECD+YGTVDFIFGNAAVVLQN
Sbjct: 361 IKHQAVALRVSADMTTFYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQN 420

Query: 421 CNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEY 480
           CN+YARLPLSGQFNALTAQGRTD NQNTG  IH CTIKPT +LAASPATKSYLGRPWKE+
Sbjct: 421 CNIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPATKSYLGRPWKEF 480

Query: 481 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATV 540
           SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEF NSGPGSDTSRR +WAVGVINATV
Sbjct: 481 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATV 540

Query: 541 ASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           A+ FTVS +LAGDQWLPPT +PY+GGLIS
Sbjct: 541 ANIFTVSEILAGDQWLPPTAVPYSGGLIS 552

BLAST of MS023385 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 524.2 bits (1349), Expect = 1.8e-147
Identity = 289/576 (50.17%), Postives = 379/576 (65.80%), Query Frame = 0

Query: 14  ILFFFLLSSPSHSAAPVTTVE------------ICQSATSPSFCRSFLPASPAPIHSHCR 73
           ++F F L+  S   +P  + +            IC+ A  PS+CRS LP  P  I+S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 74  FTLAHALARAHTFLGLVDAHLGLAPTLPTR-IVSALHDCHSLAAANVEFISNTLDTVNNT 133
            +L  +L+RA  F+ ++DA L     +  +  V AL DC  LA+  ++++ ++  T  ++
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTA-DS 124

Query: 134 TQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYS 193
           T+ L    A+++ +F+SA ITN  TC EGL   A+    S           L  D  +Y 
Sbjct: 125 TKTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLS---------GDLFNDTKLYG 184

Query: 194 LYLSLFKMGWVPENMKA-----PGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPA 253
           + L+LF  GWVP   ++     P     K F  R+G L LKM+   RA Y+ +  RK   
Sbjct: 185 VSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRK--- 244

Query: 254 APGRRLLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSG 313
                LLQ+  +A+ + V+ IV V +NG+ ++TTINAAIAAAPN +   NGYFLI+V++G
Sbjct: 245 -----LLQS--DADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAG 304

Query: 314 VYNESVVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTIT 373
           +Y E V VPKNK+YV+MIG+G NQT+ITGN +VVDG TTFNSAT ++ G  F GVN+TI 
Sbjct: 305 LYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIR 364

Query: 374 NTAGAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAA 433
           NTAG  K QAVALR   D++ FYSC FE YQDTLY H+LRQFYRECDVYGTVDFIFGNAA
Sbjct: 365 NTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAA 424

Query: 434 VVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPAT-KSYLG 493
           VVLQNCNLY R P  GQ N +TAQGRTDPNQNTGT+IH CTI+P  +LA S  T K+YLG
Sbjct: 425 VVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLG 484

Query: 494 RPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKW-AV 553
           RPWKEYSRTV MQ++ID F++P+GW  W G   L+T YYAE++N+GPGSDT+ RV W   
Sbjct: 485 RPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGY 544

Query: 554 GVINATVASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
            VINAT ASNFTV++ L G+ W+  T +P+ GGLI+
Sbjct: 545 HVINATDASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

BLAST of MS023385 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 499.6 bits (1285), Expect = 4.7e-140
Identity = 281/580 (48.45%), Postives = 376/580 (64.83%), Query Frame = 0

Query: 7   ISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLP-ASPAPIHSHCRFTLA 66
           +SL T FI    LL S S S        IC++   P FC+S  P  S   +  + RF+L 
Sbjct: 13  LSLQTLFIASQTLLPSNSSST-------ICKTTPDPKFCKSVFPQTSQGDVREYGRFSLR 72

Query: 67  HALARAHTFLGLVDAHLGLAPTLPTR-IVSALHDCHSLAAANVEFISNTLDTVNNTT--Q 126
            +L ++  F   +D +L     L ++  V AL DC  LA+   +++  + +TVN TT  +
Sbjct: 73  KSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSK 132

Query: 127 LLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYSLY 186
            L F +ADE+ + +SA +TN  TC +G+  AA+++ +   G    V   L  D  ++S+ 
Sbjct: 133 TLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNG----VALPLINDTKLFSVS 192

Query: 187 LSLFKMGWVPENMKAPGL-----PEGKH-----FR-LRHGPLNLKMSPENRAYYDRLVHR 246
           L+LF  GWVP+  K         P+  H     FR  R+G L LKM+   RA Y+ L  R
Sbjct: 193 LALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRR 252

Query: 247 KRPAAPGRRLLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIF 306
           K        L   + +   +LV+ IV V++NG+ ++TTI  A+ +APN +    GYF+I+
Sbjct: 253 K--------LADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIY 312

Query: 307 VSSGVYNESVVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVN 366
           V+SGVY E+VV+ KNK+Y++MIG+G N+T++TGN NVVDG TTFNSAT  V    F  VN
Sbjct: 313 VTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVN 372

Query: 367 LTITNTAGAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIF 426
           +T  NTAG  KHQAVA+R SAD++ FYSC FE YQDTLY H+LRQFYRECD+YGTVDFIF
Sbjct: 373 MTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIF 432

Query: 427 GNAAVVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPAT-K 486
           GNAAVV Q+CNLY R P+  QFNA+TAQGRTDPNQNTG SIHNCTIKP  +L +S  T K
Sbjct: 433 GNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVK 492

Query: 487 SYLGRPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVK 546
           +YLGRPWKEYSRTV+MQS+ID  ++P GW+EW+G   L+T YYAE++N+G GS T+ RV 
Sbjct: 493 TYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVV 552

Query: 547 W-AVGVINATVASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           W    VIN+T A+NFTV + L GD W+  + +PY  GL+S
Sbjct: 553 WPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of MS023385 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 4.7e-140
Identity = 277/584 (47.43%), Postives = 376/584 (64.38%), Query Frame = 0

Query: 5   NPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHCRFTL 64
           +PI +L    L FFL S  + S     +  IC++   P +C+S  P S   +  +  F++
Sbjct: 3   SPIFIL--ITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSI 62

Query: 65  AHALARAHTFLGLVDAHLGL-APTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNTT-- 124
             +L+++  F+  VD ++   A      ++ AL DC  LA   ++++  + +TVN+T+  
Sbjct: 63  RKSLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAK 122

Query: 125 ---QLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNV 184
              + L F +AD++ + +SA +TN  TC EGLT AA+ + +  V +   V   L  D  +
Sbjct: 123 TSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRT--GVALPLVNDTKL 182

Query: 185 YSLYLSLFKMGWVPENMKAPGL----------PEGKHFRL-RHGPLNLKMSPENRAYYDR 244
             + L+LF  GWVP+  K  G              K FRL R+G L LKM+ + +A Y+ 
Sbjct: 183 LGVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYES 242

Query: 245 LVHRKRPAAPGRRLLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGY 304
           L  RK              +   +L++ IV V ++G+ ++T I AA+AAAPNN+    G+
Sbjct: 243 LSRRKLADGDSN---GDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGF 302

Query: 305 FLIFVSSGVYNESVVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGF 364
           FLI+V++G+Y E + + KNK+Y++MIG+G NQT++TGN +VVDG TTFNSAT  V    F
Sbjct: 303 FLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNF 362

Query: 365 FGVNLTITNTAGAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTV 424
             VN+T  NTAG  KHQAVALR  AD + FYSC FE YQDTLY H+LRQFYRECDVYGTV
Sbjct: 363 VAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTV 422

Query: 425 DFIFGNAAVVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASP 484
           DFIFGNAAVV QNCNLY R P+  QFNA+TAQGR+DPNQNTGTSI NCTIKP  +L +S 
Sbjct: 423 DFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSN 482

Query: 485 AT-KSYLGRPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTS 544
            T K+YLGRPWKEYSRTVYMQS+ID F++P GW+EW+G   L+T YYAE++N+GPGS+T+
Sbjct: 483 YTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTT 542

Query: 545 RRVKW-AVGVINATVASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
            RV W    VIN+T A+NFTV+ L     W+  T +PYT GLIS
Sbjct: 543 NRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of MS023385 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 7.1e-112
Identity = 239/557 (42.91%), Postives = 335/557 (60.14%), Query Frame = 0

Query: 23  PSHSAAPVTTVEICQSATSPSFCRSFLPA---SPAPIHSHCRFTLAHALARAHTFLGLVD 82
           PS S +P      C+S   P  CR+ L A   SP+  + + +FT+   L +A     ++ 
Sbjct: 73  PSQSQSPSLA---CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVIT 132

Query: 83  AHLGLAPTLP----TRIVSALHDCHSLAAANVEFISNTLDTVNNTTQLLPFYEADELLSF 142
           ++     + P       + A+ DC  L+  +V ++  T+ T   T Q++     + + S 
Sbjct: 133 SYARRVESKPGSATAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSL 192

Query: 143 ISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYSLYLSLFKMGWVPENM 202
           +S ++TN  TC +GL V A +  ++ +GS    L  L      YS+ L L     +  N+
Sbjct: 193 LSGVVTNQQTCLDGL-VEAKSGFAAAIGSPMGNLTRL------YSISLGLVSHA-LNRNL 252

Query: 203 KAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRRLLQTNYEAEG-ILVN 262
           K     +GK     +      +    +       + K      R L +    + G ILV+
Sbjct: 253 KRFKASKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVS 312

Query: 263 GIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNESVVVPKNKKYVLMIG 322
             V V    S ++TTI  AIAAAPNN+   +GYF+I+   GVY E +VVP NKK ++++G
Sbjct: 313 KAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMG 372

Query: 323 EGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGAIKHQAVALRVSADM 382
           +G N+TIITGN+NVVDG TT+N ++  V G  F  V++T  NTAG  KHQAVALR +A+ 
Sbjct: 373 DGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEG 432

Query: 383 TTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYARLPLSGQFN 442
           ++FY C FEGYQDTLY H+LRQFYRECD+YGTVDFIFGNAA + QNCN+YAR P++ Q N
Sbjct: 433 SSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKN 492

Query: 443 ALTAQGRTDPNQNTGTSIHNCTIKPTAELAASP-ATKSYLGRPWKEYSRTVYMQSFIDAF 502
           A+TA GR DPNQNTG SI NCTIK   +LAA P +  ++LGRPWK YSRTV+MQS+I   
Sbjct: 493 AITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDI 552

Query: 503 IDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKW-AVGVINATVASNFTVSSLLAG 562
           + P GW EW+GT+ L+T YY E+SN GPG++T++RV+W    ++N   A NFTV +   G
Sbjct: 553 VQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMG 612

Query: 563 DQWLPPTTLPYTGGLIS 570
           D WLP T +P+ GGL+S
Sbjct: 613 DTWLPQTDIPFYGGLLS 617

BLAST of MS023385 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 4.8e-108
Identity = 234/563 (41.56%), Postives = 331/563 (58.79%), Query Frame = 0

Query: 22  SPSHSAAPVTTVEICQSATSPSFCRSFLPA---SPAPIHSHCRFTLAHALARA------- 81
           SPS   +P+     C+S   P  CR+ L A   SP+  + + +FT+   L +A       
Sbjct: 75  SPS-QPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVI 134

Query: 82  HTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNTTQLLPFYEADE 141
           + F   V+A  G   T     VSA+ DC  LA  +VE++    + +    +L+     D 
Sbjct: 135 NRFAQRVEADPG---TSTVEEVSAVADCGELAELSVEYLETVTEEL-KAAELMTAALVDR 194

Query: 142 LLSFISALITNGDTCYEGLTVAA---ATTTSSVVGSVEKVLAALSYDKNVYSLYLSLFKM 201
           + S +  ++TN  TC +GL  A    AT   + +G++ +          +YS+ L L   
Sbjct: 195 VTSLLGGVVTNQQTCLDGLVDAKSGFATAIGTPLGNLTR----------LYSVSLGLVSH 254

Query: 202 GWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRRLLQTNYEA 261
             +  N+K     +GK F   + P+   +    +         K      R L +    +
Sbjct: 255 A-LNRNLKRYKGSKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETS 314

Query: 262 EG-ILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNESVVVPKNK 321
            G ILV   V V    + ++ TI  A+AAAPN++    GYF+I+  +G+Y E VV+   K
Sbjct: 315 GGSILVREAVTVGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKK 374

Query: 322 KYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGAIKHQAVA 381
           + +++IG+G N+TII+GN++ +DG TT+NS+T  V G  F  V++T  NTAG  KHQAVA
Sbjct: 375 RNIMLIGDGINKTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVA 434

Query: 382 LRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYARL 441
           +R +AD +TFY C FEGYQDTLY H+LRQFYRECD+YGT+DFIFGNAA + QNCN+YAR 
Sbjct: 435 VRNNADGSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARK 494

Query: 442 PLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASP-ATKSYLGRPWKEYSRTVYM 501
           P++ Q NA+TA GRTDPNQ TG SI NCTI    +LAA P +T ++LGRPWK YSRTVY+
Sbjct: 495 PMANQKNAVTAHGRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYI 554

Query: 502 QSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWA-VGVINATVASNFT 561
           QS+I   + P GW EW+GT  L+T  Y E+ N GPG+DTS+RV+W+   ++N   A NFT
Sbjct: 555 QSYISDVVQPVGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFT 614

Query: 562 VSSLLAGDQWLPPTTLPYTGGLI 569
           V +   GD WLP T +P+ GGL+
Sbjct: 615 VYNFTLGDTWLPQTDIPFYGGLL 621

BLAST of MS023385 vs. ExPASy TrEMBL
Match: A0A6J1DL06 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111021503 PE=3 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 558/569 (98.07%), Postives = 563/569 (98.95%), Query Frame = 0

Query: 1   MASSNPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHC 60
           MASSNPISLLTFFILFFFLLSSPSHS APVTTVEICQSATSPSFCRSFLPAS A +HSHC
Sbjct: 1   MASSNPISLLTFFILFFFLLSSPSHSTAPVTTVEICQSATSPSFCRSFLPASLASVHSHC 60

Query: 61  RFTLAHALARAHTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNT 120
           RFTLAHALARAH FLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFIS+TLDTVNNT
Sbjct: 61  RFTLAHALARAHAFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISSTLDTVNNT 120

Query: 121 TQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYS 180
           TQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYS
Sbjct: 121 TQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYS 180

Query: 181 LYLSLFKMGWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRR 240
           LYLSLFKMGWVPENMKAPGLPEGKHFRLR GPLNLKMSPENRAYYDRLVHRKRPAAPGRR
Sbjct: 181 LYLSLFKMGWVPENMKAPGLPEGKHFRLRRGPLNLKMSPENRAYYDRLVHRKRPAAPGRR 240

Query: 241 LLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNES 300
           LLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNES
Sbjct: 241 LLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNES 300

Query: 301 VVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGA 360
           VVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGA
Sbjct: 301 VVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGA 360

Query: 361 IKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQN 420
           IKHQAVALRVSAD+TTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVV+QN
Sbjct: 361 IKHQAVALRVSADLTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVIQN 420

Query: 421 CNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEY 480
           CNLY RLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEY
Sbjct: 421 CNLYTRLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEY 480

Query: 481 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATV 540
           SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATV
Sbjct: 481 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATV 540

Query: 541 ASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           ASNFTVSSLLAGDQWLPPTT+PYTGGLIS
Sbjct: 541 ASNFTVSSLLAGDQWLPPTTVPYTGGLIS 569

BLAST of MS023385 vs. ExPASy TrEMBL
Match: A0A6J1EJM8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 9.6e-245
Identity = 434/568 (76.41%), Postives = 490/568 (86.27%), Query Frame = 0

Query: 2   ASSNPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHCR 61
           +SSNPISLL FF L F   SS   SA P  TVE+CQSATSPSFC+SFLP+SPA +HSHCR
Sbjct: 3   SSSNPISLLLFFSLLFLFTSS---SAVP--TVEVCQSATSPSFCKSFLPSSPASVHSHCR 62

Query: 62  FTLAHALARAHTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNTT 121
           +TL HAL +A TF+ LVD  L L P+L     SALHDC SLA AN++FIS+T+DTVN TT
Sbjct: 63  YTLHHALVQARTFVTLVDLQLTLFPSL-----SALHDCRSLAVANLDFISSTVDTVNTTT 122

Query: 122 QLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYSL 181
             LPFY+ADE++S +SA+ITN DTC++GL  +        +G V+KV+ A+S+D+ +YSL
Sbjct: 123 NALPFYQADEMISLLSAVITNIDTCHDGLAGSGLG-----LGFVDKVVKAISFDRQLYSL 182

Query: 182 YLSLFKMGWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRRL 241
           YLSLFKMGWV +NMKAP LP+  HF    GPL LKMSP++RAYY+RLVH K+PAA  RRL
Sbjct: 183 YLSLFKMGWVSKNMKAPKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVHGKKPAA--RRL 242

Query: 242 LQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNESV 301
           LQTNY  +GILVNG+VAVD+NG+YD+TTIN AIAAAPN S A NGYFLIFV++G+Y ESV
Sbjct: 243 LQTNYADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESV 302

Query: 302 VVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGAI 361
           VVPK+KKYVLMIGEGNN+TIITGN NVVDGSTTFNSATV+VEGTGFFG NLTITNTAGAI
Sbjct: 303 VVPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAI 362

Query: 362 KHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQNC 421
           KHQAVALRVSADMTTFYSCIFEGYQDTLYAH+LRQFYRECD+YGTVDFIFGNAAVVLQNC
Sbjct: 363 KHQAVALRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNC 422

Query: 422 NLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEYS 481
           N+YARLPLSGQFNALTAQGRTD NQNTG  IH CTIKPT +LAASP TKSYLGRPWKE+S
Sbjct: 423 NIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFS 482

Query: 482 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATVA 541
           RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEF NSGPGSDTS+R +WAVGVINATVA
Sbjct: 483 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVA 542

Query: 542 SNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           + FTVS +LAGDQWLPPT +PY+GGLIS
Sbjct: 543 NIFTVSEILAGDQWLPPTAVPYSGGLIS 553

BLAST of MS023385 vs. ExPASy TrEMBL
Match: A0A6J1I6B8 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.7e-244
Identity = 430/568 (75.70%), Postives = 490/568 (86.27%), Query Frame = 0

Query: 2   ASSNPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHCR 61
           +SSNPISLLTFF L F  L +   S++ V TVE+CQSATSPSFC+SFLP+SPA +HSHCR
Sbjct: 3   SSSNPISLLTFFSLLFLFLFT---SSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCR 62

Query: 62  FTLAHALARAHTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNTT 121
           +TL HAL +A TF+ LVD  L L P+      SALHDC SLA AN++FIS+T++TVN TT
Sbjct: 63  YTLHHALVQARTFITLVDLQLTLLPSF-----SALHDCRSLAMANLDFISSTVNTVNTTT 122

Query: 122 QLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYSL 181
             LPFY+ADE++S +SA+ITN DTC++GL         S +G V+KV+ A+S+D+ +YSL
Sbjct: 123 NALPFYQADEMISLLSAVITNVDTCHDGL-------AGSGLGLVDKVVKAISFDRQLYSL 182

Query: 182 YLSLFKMGWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRRL 241
           YLSLFKMGWV + MKAP LP+  HF   +GPL LKMSP++RAYY+RL+H K+PAA  RRL
Sbjct: 183 YLSLFKMGWVSKTMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAA--RRL 242

Query: 242 LQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNESV 301
           LQTN   +GILVNG+VAVD+NG+YD+TTIN AIAAAPN S A NGYFLIFV++G+Y ESV
Sbjct: 243 LQTNNADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESV 302

Query: 302 VVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGAI 361
           VVPK+KKYVLMIGEGNN+TIITGN NVVDGSTTFNSATV+VEGTGFFG NLTITNTAGAI
Sbjct: 303 VVPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAI 362

Query: 362 KHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQNC 421
           KHQAVALRVSADMTTFYSCIFEGYQDTLYAH+LRQFYRECD+YGTVDFIFGNAAVVLQNC
Sbjct: 363 KHQAVALRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNC 422

Query: 422 NLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWKEYS 481
           N+YARLPLSGQFNALTAQGRTD NQNTG  IH CTIKPT +LAASP TKSYLGRPWKE+S
Sbjct: 423 NIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFS 482

Query: 482 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINATVA 541
           RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEF NSGPGSDTSRR +WAVGVINATVA
Sbjct: 483 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVA 542

Query: 542 SNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           + FTVS +LAGDQWLPPT +PY+GGLIS
Sbjct: 543 NIFTVSEILAGDQWLPPTAVPYSGGLIS 553

BLAST of MS023385 vs. ExPASy TrEMBL
Match: A0A5A7TRW1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002210 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.1e-237
Identity = 423/571 (74.08%), Postives = 480/571 (84.06%), Query Frame = 0

Query: 1   MASSNPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHC 60
           M SSNPIS+LTF  L FFL  SPS SA P  T EICQS TSP FC  FLP SPA +HSHC
Sbjct: 1   MVSSNPISVLTFLTLLFFL--SPSSSAVP--TAEICQSTTSPPFCNFFLPNSPASVHSHC 60

Query: 61  RFTLAHALARAHTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNN- 120
           RFTL HAL  A TFL LV+AHL L P+L     S LHDC SLA AN++F+S T   VN+ 
Sbjct: 61  RFTLHHALVHARTFLSLVNAHLNLFPSL-----SPLHDCRSLAEANLDFLSQTFSIVNST 120

Query: 121 TTQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVY 180
           TT LLP+Y+A E L+ +SA+ITN DTCYEGL         S VG V+KVL A+S+DK +Y
Sbjct: 121 TTTLLPYYDAHEKLTLMSAVITNEDTCYEGL-----AGLDSAVGLVDKVLEAISFDKKLY 180

Query: 181 SLYLSLFKMGWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPG- 240
           SLYLSLFKMGWV +++KAP LP+ KHF +  G L LKMSP++RAYY+RLVHR +P  PG 
Sbjct: 181 SLYLSLFKMGWVSKDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKP--PGA 240

Query: 241 RRLLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYN 300
           RRLLQTNY+ +GI++NGIV VD+NG+YD+T I AAIAAAPN +    GYFLIFV++G+YN
Sbjct: 241 RRLLQTNYQDDGIVINGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYN 300

Query: 301 ESVVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTA 360
           E+V++PK K+YVL+IGEGNNQTIITGN NVVDGSTTFNSATV VEGTGF GVNLTI+NTA
Sbjct: 301 ETVLIPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTA 360

Query: 361 GAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVL 420
           GA KHQAVALRVSAD  T Y+CIFEGYQDTLY H+LRQFYRECD+YGTVDFIFGNAAVVL
Sbjct: 361 GAAKHQAVALRVSADNATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVL 420

Query: 421 QNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWK 480
           QNCN+YARLPLSGQFNA+TAQGRTDPNQNTGTSIHNCTIK T ELAASPATKSYLGRPWK
Sbjct: 421 QNCNIYARLPLSGQFNAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWK 480

Query: 481 EYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINA 540
           +YSRTVYMQSFID+FIDPAGWKEWDGT+NLNTSYYAEF+NSGPGSDTS+RV WAVGVINA
Sbjct: 481 QYSRTVYMQSFIDSFIDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINA 540

Query: 541 TVASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           TVA++FTVS  LAGD+WLPPT +PYTGGLIS
Sbjct: 541 TVANSFTVSQFLAGDRWLPPTDVPYTGGLIS 555

BLAST of MS023385 vs. ExPASy TrEMBL
Match: A0A1S3C644 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497470 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.1e-237
Identity = 423/571 (74.08%), Postives = 480/571 (84.06%), Query Frame = 0

Query: 1   MASSNPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHC 60
           M SSNPIS+LTF  L FFL  SPS SA P  T EICQS TSP FC  FLP SPA +HSHC
Sbjct: 1   MVSSNPISVLTFLTLLFFL--SPSSSAVP--TAEICQSTTSPPFCNFFLPNSPASVHSHC 60

Query: 61  RFTLAHALARAHTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNN- 120
           RFTL HAL  A TFL LV+AHL L P+L     S LHDC SLA AN++F+S T   VN+ 
Sbjct: 61  RFTLHHALVHARTFLSLVNAHLNLFPSL-----SPLHDCRSLAEANLDFLSQTFSIVNST 120

Query: 121 TTQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVY 180
           TT LLP+Y+A E L+ +SA+ITN DTCYEGL         S VG V+KVL A+S+DK +Y
Sbjct: 121 TTTLLPYYDAHEKLTLMSAVITNEDTCYEGL-----AGLDSAVGLVDKVLEAISFDKKLY 180

Query: 181 SLYLSLFKMGWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPG- 240
           SLYLSLFKMGWV +++KAP LP+ KHF +  G L LKMSP++RAYY+RLVHR +P  PG 
Sbjct: 181 SLYLSLFKMGWVSKDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKP--PGA 240

Query: 241 RRLLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYN 300
           RRLLQTNY+ +GI++NGIV VD+NG+YD+T I AAIAAAPN +    GYFLIFV++G+YN
Sbjct: 241 RRLLQTNYQDDGIVINGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYN 300

Query: 301 ESVVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTA 360
           E+V++PK K+YVL+IGEGNNQTIITGN NVVDGSTTFNSATV VEGTGF GVNLTI+NTA
Sbjct: 301 ETVLIPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTA 360

Query: 361 GAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVL 420
           GA KHQAVALRVSAD  T Y+CIFEGYQDTLY H+LRQFYRECD+YGTVDFIFGNAAVVL
Sbjct: 361 GAAKHQAVALRVSADNATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVL 420

Query: 421 QNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPATKSYLGRPWK 480
           QNCN+YARLPLSGQFNA+TAQGRTDPNQNTGTSIHNCTIK T ELAASPATKSYLGRPWK
Sbjct: 421 QNCNIYARLPLSGQFNAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWK 480

Query: 481 EYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWAVGVINA 540
           +YSRTVYMQSFID+FIDPAGWKEWDGT+NLNTSYYAEF+NSGPGSDTS+RV WAVGVINA
Sbjct: 481 QYSRTVYMQSFIDSFIDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINA 540

Query: 541 TVASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           TVA++FTVS  LAGD+WLPPT +PYTGGLIS
Sbjct: 541 TVANSFTVSQFLAGDRWLPPTDVPYTGGLIS 555

BLAST of MS023385 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 524.2 bits (1349), Expect = 1.3e-148
Identity = 289/576 (50.17%), Postives = 379/576 (65.80%), Query Frame = 0

Query: 14  ILFFFLLSSPSHSAAPVTTVE------------ICQSATSPSFCRSFLPASPAPIHSHCR 73
           ++F F L+  S   +P  + +            IC+ A  PS+CRS LP  P  I+S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 74  FTLAHALARAHTFLGLVDAHLGLAPTLPTR-IVSALHDCHSLAAANVEFISNTLDTVNNT 133
            +L  +L+RA  F+ ++DA L     +  +  V AL DC  LA+  ++++ ++  T  ++
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTA-DS 124

Query: 134 TQLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYS 193
           T+ L    A+++ +F+SA ITN  TC EGL   A+    S           L  D  +Y 
Sbjct: 125 TKTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLS---------GDLFNDTKLYG 184

Query: 194 LYLSLFKMGWVPENMKA-----PGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPA 253
           + L+LF  GWVP   ++     P     K F  R+G L LKM+   RA Y+ +  RK   
Sbjct: 185 VSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRK--- 244

Query: 254 APGRRLLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSG 313
                LLQ+  +A+ + V+ IV V +NG+ ++TTINAAIAAAPN +   NGYFLI+V++G
Sbjct: 245 -----LLQS--DADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAG 304

Query: 314 VYNESVVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTIT 373
           +Y E V VPKNK+YV+MIG+G NQT+ITGN +VVDG TTFNSAT ++ G  F GVN+TI 
Sbjct: 305 LYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIR 364

Query: 374 NTAGAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAA 433
           NTAG  K QAVALR   D++ FYSC FE YQDTLY H+LRQFYRECDVYGTVDFIFGNAA
Sbjct: 365 NTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAA 424

Query: 434 VVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPAT-KSYLG 493
           VVLQNCNLY R P  GQ N +TAQGRTDPNQNTGT+IH CTI+P  +LA S  T K+YLG
Sbjct: 425 VVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLG 484

Query: 494 RPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKW-AV 553
           RPWKEYSRTV MQ++ID F++P+GW  W G   L+T YYAE++N+GPGSDT+ RV W   
Sbjct: 485 RPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGY 544

Query: 554 GVINATVASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
            VINAT ASNFTV++ L G+ W+  T +P+ GGLI+
Sbjct: 545 HVINATDASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

BLAST of MS023385 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 499.6 bits (1285), Expect = 3.3e-141
Identity = 277/584 (47.43%), Postives = 376/584 (64.38%), Query Frame = 0

Query: 5   NPISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLPASPAPIHSHCRFTL 64
           +PI +L    L FFL S  + S     +  IC++   P +C+S  P S   +  +  F++
Sbjct: 3   SPIFIL--ITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSI 62

Query: 65  AHALARAHTFLGLVDAHLGL-APTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNTT-- 124
             +L+++  F+  VD ++   A      ++ AL DC  LA   ++++  + +TVN+T+  
Sbjct: 63  RKSLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAK 122

Query: 125 ---QLLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNV 184
              + L F +AD++ + +SA +TN  TC EGLT AA+ + +  V +   V   L  D  +
Sbjct: 123 TSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRT--GVALPLVNDTKL 182

Query: 185 YSLYLSLFKMGWVPENMKAPGL----------PEGKHFRL-RHGPLNLKMSPENRAYYDR 244
             + L+LF  GWVP+  K  G              K FRL R+G L LKM+ + +A Y+ 
Sbjct: 183 LGVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYES 242

Query: 245 LVHRKRPAAPGRRLLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGY 304
           L  RK              +   +L++ IV V ++G+ ++T I AA+AAAPNN+    G+
Sbjct: 243 LSRRKLADGDSN---GDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGF 302

Query: 305 FLIFVSSGVYNESVVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGF 364
           FLI+V++G+Y E + + KNK+Y++MIG+G NQT++TGN +VVDG TTFNSAT  V    F
Sbjct: 303 FLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNF 362

Query: 365 FGVNLTITNTAGAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTV 424
             VN+T  NTAG  KHQAVALR  AD + FYSC FE YQDTLY H+LRQFYRECDVYGTV
Sbjct: 363 VAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTV 422

Query: 425 DFIFGNAAVVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASP 484
           DFIFGNAAVV QNCNLY R P+  QFNA+TAQGR+DPNQNTGTSI NCTIKP  +L +S 
Sbjct: 423 DFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSN 482

Query: 485 AT-KSYLGRPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTS 544
            T K+YLGRPWKEYSRTVYMQS+ID F++P GW+EW+G   L+T YYAE++N+GPGS+T+
Sbjct: 483 YTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTT 542

Query: 545 RRVKW-AVGVINATVASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
            RV W    VIN+T A+NFTV+ L     W+  T +PYT GLIS
Sbjct: 543 NRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of MS023385 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 499.6 bits (1285), Expect = 3.3e-141
Identity = 281/580 (48.45%), Postives = 376/580 (64.83%), Query Frame = 0

Query: 7   ISLLTFFILFFFLLSSPSHSAAPVTTVEICQSATSPSFCRSFLP-ASPAPIHSHCRFTLA 66
           +SL T FI    LL S S S        IC++   P FC+S  P  S   +  + RF+L 
Sbjct: 13  LSLQTLFIASQTLLPSNSSST-------ICKTTPDPKFCKSVFPQTSQGDVREYGRFSLR 72

Query: 67  HALARAHTFLGLVDAHLGLAPTLPTR-IVSALHDCHSLAAANVEFISNTLDTVNNTT--Q 126
            +L ++  F   +D +L     L ++  V AL DC  LA+   +++  + +TVN TT  +
Sbjct: 73  KSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSK 132

Query: 127 LLPFYEADELLSFISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYSLY 186
            L F +ADE+ + +SA +TN  TC +G+  AA+++ +   G    V   L  D  ++S+ 
Sbjct: 133 TLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNG----VALPLINDTKLFSVS 192

Query: 187 LSLFKMGWVPENMKAPGL-----PEGKH-----FR-LRHGPLNLKMSPENRAYYDRLVHR 246
           L+LF  GWVP+  K         P+  H     FR  R+G L LKM+   RA Y+ L  R
Sbjct: 193 LALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRR 252

Query: 247 KRPAAPGRRLLQTNYEAEGILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIF 306
           K        L   + +   +LV+ IV V++NG+ ++TTI  A+ +APN +    GYF+I+
Sbjct: 253 K--------LADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIY 312

Query: 307 VSSGVYNESVVVPKNKKYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVN 366
           V+SGVY E+VV+ KNK+Y++MIG+G N+T++TGN NVVDG TTFNSAT  V    F  VN
Sbjct: 313 VTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVN 372

Query: 367 LTITNTAGAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIF 426
           +T  NTAG  KHQAVA+R SAD++ FYSC FE YQDTLY H+LRQFYRECD+YGTVDFIF
Sbjct: 373 MTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIF 432

Query: 427 GNAAVVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPAT-K 486
           GNAAVV Q+CNLY R P+  QFNA+TAQGRTDPNQNTG SIHNCTIKP  +L +S  T K
Sbjct: 433 GNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVK 492

Query: 487 SYLGRPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVK 546
           +YLGRPWKEYSRTV+MQS+ID  ++P GW+EW+G   L+T YYAE++N+G GS T+ RV 
Sbjct: 493 TYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVV 552

Query: 547 W-AVGVINATVASNFTVSSLLAGDQWLPPTTLPYTGGLIS 570
           W    VIN+T A+NFTV + L GD W+  + +PY  GL+S
Sbjct: 553 WPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of MS023385 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 406.0 bits (1042), Expect = 5.0e-113
Identity = 239/557 (42.91%), Postives = 335/557 (60.14%), Query Frame = 0

Query: 23  PSHSAAPVTTVEICQSATSPSFCRSFLPA---SPAPIHSHCRFTLAHALARAHTFLGLVD 82
           PS S +P      C+S   P  CR+ L A   SP+  + + +FT+   L +A     ++ 
Sbjct: 73  PSQSQSPSLA---CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVIT 132

Query: 83  AHLGLAPTLP----TRIVSALHDCHSLAAANVEFISNTLDTVNNTTQLLPFYEADELLSF 142
           ++     + P       + A+ DC  L+  +V ++  T+ T   T Q++     + + S 
Sbjct: 133 SYARRVESKPGSATAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSL 192

Query: 143 ISALITNGDTCYEGLTVAAATTTSSVVGSVEKVLAALSYDKNVYSLYLSLFKMGWVPENM 202
           +S ++TN  TC +GL V A +  ++ +GS    L  L      YS+ L L     +  N+
Sbjct: 193 LSGVVTNQQTCLDGL-VEAKSGFAAAIGSPMGNLTRL------YSISLGLVSHA-LNRNL 252

Query: 203 KAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRRLLQTNYEAEG-ILVN 262
           K     +GK     +      +    +       + K      R L +    + G ILV+
Sbjct: 253 KRFKASKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVS 312

Query: 263 GIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNESVVVPKNKKYVLMIG 322
             V V    S ++TTI  AIAAAPNN+   +GYF+I+   GVY E +VVP NKK ++++G
Sbjct: 313 KAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMG 372

Query: 323 EGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGAIKHQAVALRVSADM 382
           +G N+TIITGN+NVVDG TT+N ++  V G  F  V++T  NTAG  KHQAVALR +A+ 
Sbjct: 373 DGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEG 432

Query: 383 TTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYARLPLSGQFN 442
           ++FY C FEGYQDTLY H+LRQFYRECD+YGTVDFIFGNAA + QNCN+YAR P++ Q N
Sbjct: 433 SSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKN 492

Query: 443 ALTAQGRTDPNQNTGTSIHNCTIKPTAELAASP-ATKSYLGRPWKEYSRTVYMQSFIDAF 502
           A+TA GR DPNQNTG SI NCTIK   +LAA P +  ++LGRPWK YSRTV+MQS+I   
Sbjct: 493 AITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDI 552

Query: 503 IDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKW-AVGVINATVASNFTVSSLLAG 562
           + P GW EW+GT+ L+T YY E+SN GPG++T++RV+W    ++N   A NFTV +   G
Sbjct: 553 VQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMG 612

Query: 563 DQWLPPTTLPYTGGLIS 570
           D WLP T +P+ GGL+S
Sbjct: 613 DTWLPQTDIPFYGGLLS 617

BLAST of MS023385 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 393.3 bits (1009), Expect = 3.4e-109
Identity = 234/563 (41.56%), Postives = 331/563 (58.79%), Query Frame = 0

Query: 22  SPSHSAAPVTTVEICQSATSPSFCRSFLPA---SPAPIHSHCRFTLAHALARA------- 81
           SPS   +P+     C+S   P  CR+ L A   SP+  + + +FT+   L +A       
Sbjct: 75  SPS-QPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVI 134

Query: 82  HTFLGLVDAHLGLAPTLPTRIVSALHDCHSLAAANVEFISNTLDTVNNTTQLLPFYEADE 141
           + F   V+A  G   T     VSA+ DC  LA  +VE++    + +    +L+     D 
Sbjct: 135 NRFAQRVEADPG---TSTVEEVSAVADCGELAELSVEYLETVTEEL-KAAELMTAALVDR 194

Query: 142 LLSFISALITNGDTCYEGLTVAA---ATTTSSVVGSVEKVLAALSYDKNVYSLYLSLFKM 201
           + S +  ++TN  TC +GL  A    AT   + +G++ +          +YS+ L L   
Sbjct: 195 VTSLLGGVVTNQQTCLDGLVDAKSGFATAIGTPLGNLTR----------LYSVSLGLVSH 254

Query: 202 GWVPENMKAPGLPEGKHFRLRHGPLNLKMSPENRAYYDRLVHRKRPAAPGRRLLQTNYEA 261
             +  N+K     +GK F   + P+   +    +         K      R L +    +
Sbjct: 255 A-LNRNLKRYKGSKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETS 314

Query: 262 EG-ILVNGIVAVDKNGSYDYTTINAAIAAAPNNSAAGNGYFLIFVSSGVYNESVVVPKNK 321
            G ILV   V V    + ++ TI  A+AAAPN++    GYF+I+  +G+Y E VV+   K
Sbjct: 315 GGSILVREAVTVGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKK 374

Query: 322 KYVLMIGEGNNQTIITGNNNVVDGSTTFNSATVVVEGTGFFGVNLTITNTAGAIKHQAVA 381
           + +++IG+G N+TII+GN++ +DG TT+NS+T  V G  F  V++T  NTAG  KHQAVA
Sbjct: 375 RNIMLIGDGINKTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVA 434

Query: 382 LRVSADMTTFYSCIFEGYQDTLYAHTLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYARL 441
           +R +AD +TFY C FEGYQDTLY H+LRQFYRECD+YGT+DFIFGNAA + QNCN+YAR 
Sbjct: 435 VRNNADGSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARK 494

Query: 442 PLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASP-ATKSYLGRPWKEYSRTVYM 501
           P++ Q NA+TA GRTDPNQ TG SI NCTI    +LAA P +T ++LGRPWK YSRTVY+
Sbjct: 495 PMANQKNAVTAHGRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYI 554

Query: 502 QSFIDAFIDPAGWKEWDGTLNLNTSYYAEFSNSGPGSDTSRRVKWA-VGVINATVASNFT 561
           QS+I   + P GW EW+GT  L+T  Y E+ N GPG+DTS+RV+W+   ++N   A NFT
Sbjct: 555 QSYISDVVQPVGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFT 614

Query: 562 VSSLLAGDQWLPPTTLPYTGGLI 569
           V +   GD WLP T +P+ GGL+
Sbjct: 615 VYNFTLGDTWLPQTDIPFYGGLL 621