MS023380 (gene) Bitter gourd (TR) v1

BLAST of MS023380 vs. NCBI nr
Match: XP_022154172.1 (UPF0496 protein At2g18630-like [Momordica charantia])

HSP 1 Score: 643.7 bits (1659), Expect = 9.3e-181
Identity = 332/339 (97.94%), Postives = 334/339 (98.53%), Query Frame = 0

Query: 7   SSHDGADLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEMNQSVAKIVLE 66
           +SHDGADL FFDVVVQERTTGVVNSLA EVGAWSLSMDELNEIIEHPLEMNQSVAKIVLE
Sbjct: 43  NSHDGADLPFFDVVVQERTTGVVNSLATEVGAWSLSMDELNEIIEHPLEMNQSVAKIVLE 102

Query: 67  CKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAVKQFEEDQKGG 126
           CKKDMWSNMEMFHLSNAFFDNSIKTSEFC VSENC +RTRNSQLIIKLAVKQFEEDQKGG
Sbjct: 103 CKKDMWSNMEMFHLSNAFFDNSIKTSEFCXVSENCPQRTRNSQLIIKLAVKQFEEDQKGG 162

Query: 127 DEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRKLDKKSNTMKAW 186
           DEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRKLDKKSNTMKAW
Sbjct: 163 DEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRKLDKKSNTMKAW 222

Query: 187 KKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWCNTIWNRHLKRI 246
           KKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWCNTIWNRHLKRI
Sbjct: 223 KKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWCNTIWNRHLKRI 282

Query: 247 EKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLLANLCIMEQGAMQLVVNEIKKNL 306
           EKEKQLIISMQRPTF VTKELEEIRVLVPILSNRFGLLANLCIMEQGAMQLVVNEIKKNL
Sbjct: 283 EKEKQLIISMQRPTFTVTKELEEIRVLVPILSNRFGLLANLCIMEQGAMQLVVNEIKKNL 342

Query: 307 QGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHPAA 346
           QGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHPAA
Sbjct: 343 QGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHPAA 381

BLAST of MS023380 vs. NCBI nr
Match: XP_022154193.1 (UPF0496 protein At2g18630-like [Momordica charantia])

HSP 1 Score: 436.4 bits (1121), Expect = 2.3e-118
Identity = 237/350 (67.71%), Postives = 278/350 (79.43%), Query Frame = 0

Query: 2   YAANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEM 61
           YAA+LSS++ A     DLQ FDV V ERTT V+NSLA  V A SLS D L E+ +  LEM
Sbjct: 28  YAADLSSYEAACENDPDLQSFDVAVHERTTRVLNSLATGVEARSLSFDALMEVTDCLLEM 87

Query: 62  NQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAV 121
           NQ V KI+LE K+D+WSN EMF L N FFDNS+KT +FCT  ENCLRRTR+SQ IIKLAV
Sbjct: 88  NQDVVKIILESKEDVWSNKEMFKLVNEFFDNSLKTLDFCTGLENCLRRTRDSQFIIKLAV 147

Query: 122 KQFEEDQKGGDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRKL 181
           KQ EE + GGDE+ VRTFEELKKF+EAG+PFT EFVQL QSLY+QH S F KLQ EKR+L
Sbjct: 148 KQLEEVENGGDERYVRTFEELKKFQEAGDPFTLEFVQLFQSLYKQHLSMFKKLQTEKRRL 207

Query: 182 DKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWCN 241
           DKK NT K WKK SN+IFVTAF SV+IFS+VAA  SAPP+VIALAAA A+P+GPV KWCN
Sbjct: 208 DKKYNTTKTWKKVSNVIFVTAFASVLIFSVVAAAMSAPPVVIALAAALAVPMGPVGKWCN 267

Query: 242 TIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANLCIMEQG 301
           ++WNR+LK+I++EKQLI SMQ  T+ + K+ E IRVLV  LS +FG L   ANL I EQG
Sbjct: 268 SMWNRYLKKIKEEKQLISSMQGHTYIILKDFENIRVLVRKLSIQFGSLLQNANLGIREQG 327

Query: 302 AMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHP 344
           AMQLV++EIKKNL+GFDETIEKLSVHAG+CS+ VT    T +LQKI+RHP
Sbjct: 328 AMQLVIDEIKKNLEGFDETIEKLSVHAGKCSRDVT-MARTVILQKIVRHP 376

BLAST of MS023380 vs. NCBI nr
Match: XP_022947486.1 (UPF0496 protein At2g18630-like [Cucurbita moschata] >KAG6605295.1 UPF0496 protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035254.1 UPF0496 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 379.8 bits (974), Expect = 2.5e-101
Identity = 205/350 (58.57%), Postives = 254/350 (72.57%), Query Frame = 0

Query: 1   HYAANLSSHD-----GADLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLE 60
           HYAA LSS++       DLQ FD  V ERT   +NSLA  +G  SLS+D L EI E  LE
Sbjct: 29  HYAAALSSYETECENNPDLQSFDDKVHERTNRALNSLATGIGPSSLSLDVLMEIPEFLLE 88

Query: 61  MNQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLA 120
           MN+   K++L  K+D+WSN E+F L +AFFDNS+KT  FCT  ENCLRRTR+S+LIIK+A
Sbjct: 89  MNEDAVKLILASKEDVWSNKELFELVDAFFDNSLKTLHFCTALENCLRRTRDSELIIKVA 148

Query: 121 VKQFEEDQKGGDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRK 180
           + QFE  + G DE+ V+T EELKKF+E GNPF+ EFVQL   LY+QH S F KLQ +K+K
Sbjct: 149 INQFESGENGDDERYVKTIEELKKFQEVGNPFSQEFVQLFVPLYKQHLSMFKKLQHQKKK 208

Query: 181 LDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWC 240
           LDKK  TMK WKK +N+  VT F SV++FS+VAA  SAPP+VIAL AA  +P+GPV KWC
Sbjct: 209 LDKKYRTMKTWKKLTNVFLVTLFASVLVFSVVAAAMSAPPVVIALTAALVVPMGPVGKWC 268

Query: 241 NTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANLCIMEQ 300
           NT+WNR+L  I+ EKQLI S+Q  T+ V K+ E IR+LV  LS + G L    +L I EQ
Sbjct: 269 NTLWNRYLSHIKAEKQLINSVQGHTYIVLKDFENIRLLVQKLSIQLGSLLQNTDLGIREQ 328

Query: 301 GAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRH 343
           GAMQLV++EIKKNL+GFDETIEKLSVHA +CS  VT +  T ++QKI  H
Sbjct: 329 GAMQLVIDEIKKNLKGFDETIEKLSVHASKCSTDVT-KARTVIIQKIAMH 377

BLAST of MS023380 vs. NCBI nr
Match: XP_023533689.1 (UPF0496 protein At2g18630-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375.9 bits (964), Expect = 3.6e-100
Identity = 204/350 (58.29%), Postives = 251/350 (71.71%), Query Frame = 0

Query: 1   HYAANLSSHD-----GADLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLE 60
           HYAA LSS++       DLQ FD  V ERT   +NSLA  +G  SLS+D L EI E  LE
Sbjct: 29  HYAAALSSYETECENNPDLQAFDDKVHERTNRALNSLATGIGPSSLSLDVLMEIPEFLLE 88

Query: 61  MNQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLA 120
           MN+   K++L  K+D+WSN E+F L +AFFDNS+KT  FCT  ENCLRRTR+S+LIIK+A
Sbjct: 89  MNEDAVKLILASKEDVWSNKELFELVDAFFDNSLKTLRFCTALENCLRRTRDSELIIKVA 148

Query: 121 VKQFEEDQKGGDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRK 180
           + QFE  + G DE+ V+T EELKKF+E GNPF  EFVQL   LY+QH S   KLQ +K+K
Sbjct: 149 INQFESGENGDDERYVKTIEELKKFQEVGNPFAQEFVQLFVPLYKQHLSMLKKLQHQKKK 208

Query: 181 LDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWC 240
           LDKK  TMK WKK +N+  VT F SV++FS+VAA  SAPP+VIAL AA  +P+GPV KWC
Sbjct: 209 LDKKYRTMKTWKKLTNVFLVTLFASVLVFSVVAAAMSAPPVVIALTAALVVPMGPVGKWC 268

Query: 241 NTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANLCIMEQ 300
           NT+WNR+L  I+ EKQ+I S+Q  T  V  + E IR+LV  LS + GLL    +L I EQ
Sbjct: 269 NTLWNRYLSHIKAEKQVINSVQAHTSIVLTDFENIRLLVQKLSIQLGLLLQNTDLGIREQ 328

Query: 301 GAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRH 343
           GAMQLV++EIKKNLQGFDETIEKLSVHA +CS  VT +  T ++QKI  H
Sbjct: 329 GAMQLVIDEIKKNLQGFDETIEKLSVHASKCSTDVT-KARTVIIQKIAMH 377

BLAST of MS023380 vs. NCBI nr
Match: XP_023006839.1 (UPF0496 protein At2g18630-like [Cucurbita maxima])

HSP 1 Score: 371.7 bits (953), Expect = 6.8e-99
Identity = 199/350 (56.86%), Postives = 251/350 (71.71%), Query Frame = 0

Query: 1   HYAANLSSHD-----GADLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLE 60
           HY+A LSS++       DLQ FD  + ERT   ++SLA  +G  SLS+D L EI E  LE
Sbjct: 29  HYSAALSSYETECENNPDLQSFDAKLHERTNQALHSLATGIGPGSLSLDVLMEIPEFLLE 88

Query: 61  MNQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLA 120
           MN+   K++L  K+D+WSN E+F L +AFFDNS+KT  FC   ENCLRRTR+S+LIIK+A
Sbjct: 89  MNEDAVKLILASKEDVWSNKELFELVDAFFDNSLKTLHFCAALENCLRRTRDSELIIKVA 148

Query: 121 VKQFEEDQKGGDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRK 180
           + QFE  + G DE+ V+T E LKKF+E GNPF  EFVQL   LY+QH S F KLQ +K+K
Sbjct: 149 INQFESGENGDDERYVKTIEGLKKFQEVGNPFAQEFVQLFVPLYKQHLSMFKKLQHQKKK 208

Query: 181 LDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWC 240
           LDKK  TMK WKK +N+  VT F S+++FS+VAA  SAPP+VIAL AA  +P+GPV KWC
Sbjct: 209 LDKKYRTMKTWKKLTNVFLVTLFASILVFSVVAAAMSAPPVVIALTAALVVPMGPVGKWC 268

Query: 241 NTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANLCIMEQ 300
           NT+WNR+L  I+ EKQLI S+Q  T+ V K+ E IR+LV  LS + G L    +L I EQ
Sbjct: 269 NTLWNRYLSHIKAEKQLINSVQGHTYIVLKDFENIRLLVQKLSIQLGSLLQNTDLGIREQ 328

Query: 301 GAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRH 343
           GAMQLV++EIKKNL+GFDETIEKLSVHA +CS  VT +  T ++QKI  H
Sbjct: 329 GAMQLVIDEIKKNLKGFDETIEKLSVHASKCSTDVT-KARTVIIQKIAMH 377

BLAST of MS023380 vs. ExPASy Swiss-Prot
Match: Q56XQ0 (UPF0496 protein At2g18630 OS=Arabidopsis thaliana OX=3702 GN=At2g18630 PE=2 SV=3)

HSP 1 Score: 279.6 bits (714), Expect = 4.6e-74
Identity = 155/352 (44.03%), Postives = 227/352 (64.49%), Query Frame = 0

Query: 2   YAANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEM 61
           Y  +LSS++ A      L+ FD  + ERT  V+N LA  V   SLS D L E+ +  L+M
Sbjct: 30  YTEHLSSYERACSEDPKLESFDSALHERTNRVINKLASGVEIKSLSFDSLREVTQCLLDM 89

Query: 62  NQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAV 121
           NQ V K++L+ K+D+W+N ++F L N +F+++ KT +FC+  ENCL R R SQ+II+ AV
Sbjct: 90  NQDVVKVILQDKEDIWNNQDLFSLVNLYFESTAKTMDFCSELENCLNRARRSQVIIQFAV 149

Query: 122 KQFEEDQKGGD-EKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRK 181
            QFEE+ +  +  K  +T EELK+F+ AG PFT EF  L   +Y+Q      +L   KRK
Sbjct: 150 NQFEEENEDKENRKYEKTLEELKRFKVAGEPFTKEFFALFDLVYKQQVMMLEELHKLKRK 209

Query: 182 LDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWC 241
           LDK+   +K W++ SN++FVTAFVSV+IFS+VAA  +APP+V A+A A A+P+G V KWC
Sbjct: 210 LDKRLRNIKTWRRVSNMVFVTAFVSVLIFSVVAAAVAAPPVVAAIAGALAVPVGSVGKWC 269

Query: 242 NTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANLCIMEQ 301
           NT+W ++ K +  +K++I S++  T+   KE++ I +LV  +      L   A   I E+
Sbjct: 270 NTLWTKYEKVVRGQKEIITSIRIGTYISVKEMDNISILVRKVEVEIESLLKKAEFAITEE 329

Query: 302 GAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHPA 345
             ++L ++EIKK L  F ETIE+L  HAG+    VT +  T +LQ+IIR+PA
Sbjct: 330 KEVRLAIDEIKKKLDVFTETIEELGEHAGKYCSDVT-KARTVILQRIIRYPA 380

BLAST of MS023380 vs. ExPASy Swiss-Prot
Match: Q9SYZ7 (UPF0496 protein At4g34320 OS=Arabidopsis thaliana OX=3702 GN=At4g34320 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.2e-68
Identity = 146/353 (41.36%), Postives = 223/353 (63.17%), Query Frame = 0

Query: 1   HYAANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLE 60
           HY   L S+  A     +LQ FD  +Q RT+ V+++LA  V   +LS D L E+ +  LE
Sbjct: 18  HYTTELRSYAAACKADTELQSFDTCLQARTSHVISTLATGVEVRALSFDSLKEVTQCLLE 77

Query: 61  MNQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLA 120
           MNQ V K++L+CKKD+W N EMF L   +F+NS+KT +FC   E  LRR R+S L+I +A
Sbjct: 78  MNQEVVKVILDCKKDIWKNQEMFELVEDYFENSLKTLDFCAALEKGLRRARDSHLLILVA 137

Query: 121 VKQFEED---QKGGDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIE 180
           ++QFE++   Q G   K  +T EELK F++A +PF  +F ++ QS+Y+Q      KLQ  
Sbjct: 138 LQQFEDESLVQGGNGYK--KTLEELKNFKDAESPFNEDFFKMFQSVYKQQMLMLEKLQHR 197

Query: 181 KRKLDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVA 240
           K KLDKK   +  W+K S++IFV  F +V+I S+VAA  +APP+  ALAAA A+PLG + 
Sbjct: 198 KNKLDKKLKCIHTWRKLSSIIFVATFATVLICSVVAAAMAAPPVAAALAAATAVPLGSMG 257

Query: 241 KWCNTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRF-GLLANL-CIM 300
           KW +++W  +   ++ +K++I SMQ  TF   K+L+ IRVL+  L     G++ +    +
Sbjct: 258 KWIDSLWKNYENALKGQKEVISSMQAGTFVAVKDLDNIRVLIERLEIEITGMVKSAEFAV 317

Query: 301 EQGAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHP 344
           E  A+++ +++IKK L+ F + +E+L   A  CS+ +  +  T +LQ+II+HP
Sbjct: 318 EHNAVKIGIDDIKKKLEVFKKNVEELGTQADLCSRDI-RRARTVILQRIIKHP 367

BLAST of MS023380 vs. ExPASy Swiss-Prot
Match: Q8GW16 (UPF0496 protein At5g66675 OS=Arabidopsis thaliana OX=3702 GN=At5g66675 PE=2 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 5.5e-67
Identity = 145/362 (40.06%), Postives = 222/362 (61.33%), Query Frame = 0

Query: 2   YAANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEM 61
           Y+A+L++++ A     DLQ FD  + +RT  V+NSLA      SLS D L E+    LEM
Sbjct: 38  YSADLTAYNSACREDPDLQSFDSSLHQRTNRVINSLASGAQTRSLSFDALIEVSGCLLEM 97

Query: 62  NQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAV 121
           NQ V + ++E K+D+W N ++  L NA+FD+SIKT +FC   +NC++R R  Q++++ A+
Sbjct: 98  NQEVVRFIIESKEDVWDNKDLTCLVNAYFDSSIKTLDFCNAVDNCVKRARIGQMLLQFAL 157

Query: 122 KQFEEDQKG-------GDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKL 181
           KQFE +  G       G  K  +T EEL KF+ +G+PF  +F  L +S+YEQ       L
Sbjct: 158 KQFEMESSGSNKSVEPGTNKYAKTLEELNKFKASGDPFDGDFFMLLESVYEQQVMLLEIL 217

Query: 182 QIEKRKLDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLG 241
             +KRKLDKK   +K WKK SN++FVTAFVSV+IFS+VAA  +APP+V ALAAA A+P+G
Sbjct: 218 HKQKRKLDKKLKNIKYWKKISNVVFVTAFVSVLIFSVVAAAVAAPPVVTALAAALAVPIG 277

Query: 242 PVAKWCNTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLLANLCI 301
            + KWCN +W ++   ++ +K +++SM+   +   K+++ IRV V  L      +     
Sbjct: 278 SIGKWCNHLWKKYETAVKGQKDIVLSMRIGAYVTMKDMDNIRVHVDKLKIEMESMMQKVD 337

Query: 302 M------EQGAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHP 346
                  E+ A++L ++EI K    F E IE++  +A +CSK +T    T +L+ I+  P
Sbjct: 338 FALKEKEEEVAVRLSMHEISKKFDVFTERIEEVGDNATKCSKNIT-LARTIVLRHILSFP 397

BLAST of MS023380 vs. ExPASy Swiss-Prot
Match: A2XDK8 (UPF0496 protein 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_010151 PE=2 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.3e-62
Identity = 133/362 (36.74%), Postives = 225/362 (62.15%), Query Frame = 0

Query: 3   AANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEMN 62
           A  LSS++ A     +L+ FD  +Q RT+  +++LA+ V   SLS++ L E+    L+MN
Sbjct: 29  AEELSSYEAACRSDPELRTFDTTLQRRTSRAISTLAVGVEVRSLSLESLREVTGCLLDMN 88

Query: 63  QSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAVK 122
           Q V +++L+CKKD+W + E+F L   +F++S+ T +FCT  + CL+R R+SQL++ +A++
Sbjct: 89  QEVVRVILDCKKDIWKSPELFDLVEDYFESSLHTLDFCTALDKCLKRARDSQLLLHVALQ 148

Query: 123 QFEEDQ-----KGGDE------KCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTF 182
           +F++++       G E      +  RT  EL++F+ AG+PFT EF    Q++Y Q  +  
Sbjct: 149 RFDDEEDNDAAAAGQEDAAPSARYARTLHELRQFKAAGDPFTEEFFSAFQAVYRQQLTML 208

Query: 183 NKLQIEKRKLDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAI 242
            KLQ  K +LDKK   +KAW++ S++IF T F +V+I S+VAA  +APP+  ALAAA +I
Sbjct: 209 EKLQQRKHRLDKKVRAIKAWRRVSSIIFATTFAAVLICSVVAAAIAAPPVAAALAAAASI 268

Query: 243 PLGPVAKWCNTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLLAN 302
           P+G + KW +++   +   +  +K+++ +MQ  TF   K+L+ IRVL+  +      + +
Sbjct: 269 PVGSMGKWIDSLLKGYQDALRGQKEVVSAMQVGTFIAIKDLDSIRVLINRVELEISSMID 328

Query: 303 LCI----MEQGAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRH 345
            C+     ++ A++  V EIKK L+ F +++E L   A  CS+ +  +  T +LQ+IIRH
Sbjct: 329 -CVEFAERDEEAVKFGVEEIKKKLEVFMKSVEDLGEQADRCSRDI-RRARTVVLQRIIRH 388

BLAST of MS023380 vs. ExPASy Swiss-Prot
Match: Q10QE9 (UPF0496 protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0199100 PE=2 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.3e-62
Identity = 133/362 (36.74%), Postives = 225/362 (62.15%), Query Frame = 0

Query: 3   AANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEMN 62
           A  LSS++ A     +L+ FD  +Q RT+  +++LA+ V   SLS++ L E+    L+MN
Sbjct: 29  AEELSSYEAACRSDPELRTFDTTLQRRTSRAISTLAVGVEVRSLSLESLREVTGCLLDMN 88

Query: 63  QSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAVK 122
           Q V +++L+CKKD+W + E+F L   +F++S+ T +FCT  + CL+R R+SQL++ +A++
Sbjct: 89  QEVVRVILDCKKDIWKSPELFDLVEDYFESSLHTLDFCTALDKCLKRARDSQLLLHVALQ 148

Query: 123 QFEEDQ-----KGGDE------KCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTF 182
           +F++++       G E      +  RT  EL++F+ AG+PFT EF    Q++Y Q  +  
Sbjct: 149 RFDDEEDNDAAAAGQEDAAPSARYARTLHELRQFKAAGDPFTEEFFSAFQAVYRQQLTML 208

Query: 183 NKLQIEKRKLDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAI 242
            KLQ  K +LDKK   +KAW++ S++IF T F +V+I S+VAA  +APP+  ALAAA +I
Sbjct: 209 EKLQQRKHRLDKKVRAIKAWRRVSSIIFATTFAAVLICSVVAAAIAAPPVAAALAAAASI 268

Query: 243 PLGPVAKWCNTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLLAN 302
           P+G + KW +++   +   +  +K+++ +MQ  TF   K+L+ IRVL+  +      + +
Sbjct: 269 PVGSMGKWIDSLLKGYQDALRGQKEVVSAMQVGTFIAIKDLDSIRVLINRVELEISSMID 328

Query: 303 LCI----MEQGAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRH 345
            C+     ++ A++  V EIKK L+ F +++E L   A  CS+ +  +  T +LQ+IIRH
Sbjct: 329 -CVEFAERDEEAVKFGVEEIKKKLEVFMKSVEDLGEQADRCSRDI-RRARTVVLQRIIRH 388

BLAST of MS023380 vs. ExPASy TrEMBL
Match: A0A6J1DKZ1 (UPF0496 protein At2g18630-like OS=Momordica charantia OX=3673 GN=LOC111021486 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 4.5e-181
Identity = 332/339 (97.94%), Postives = 334/339 (98.53%), Query Frame = 0

Query: 7   SSHDGADLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEMNQSVAKIVLE 66
           +SHDGADL FFDVVVQERTTGVVNSLA EVGAWSLSMDELNEIIEHPLEMNQSVAKIVLE
Sbjct: 43  NSHDGADLPFFDVVVQERTTGVVNSLATEVGAWSLSMDELNEIIEHPLEMNQSVAKIVLE 102

Query: 67  CKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAVKQFEEDQKGG 126
           CKKDMWSNMEMFHLSNAFFDNSIKTSEFC VSENC +RTRNSQLIIKLAVKQFEEDQKGG
Sbjct: 103 CKKDMWSNMEMFHLSNAFFDNSIKTSEFCXVSENCPQRTRNSQLIIKLAVKQFEEDQKGG 162

Query: 127 DEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRKLDKKSNTMKAW 186
           DEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRKLDKKSNTMKAW
Sbjct: 163 DEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRKLDKKSNTMKAW 222

Query: 187 KKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWCNTIWNRHLKRI 246
           KKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWCNTIWNRHLKRI
Sbjct: 223 KKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWCNTIWNRHLKRI 282

Query: 247 EKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLLANLCIMEQGAMQLVVNEIKKNL 306
           EKEKQLIISMQRPTF VTKELEEIRVLVPILSNRFGLLANLCIMEQGAMQLVVNEIKKNL
Sbjct: 283 EKEKQLIISMQRPTFTVTKELEEIRVLVPILSNRFGLLANLCIMEQGAMQLVVNEIKKNL 342

Query: 307 QGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHPAA 346
           QGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHPAA
Sbjct: 343 QGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHPAA 381

BLAST of MS023380 vs. ExPASy TrEMBL
Match: A0A6J1DJM4 (UPF0496 protein At2g18630-like OS=Momordica charantia OX=3673 GN=LOC111021511 PE=3 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 1.1e-118
Identity = 237/350 (67.71%), Postives = 278/350 (79.43%), Query Frame = 0

Query: 2   YAANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEM 61
           YAA+LSS++ A     DLQ FDV V ERTT V+NSLA  V A SLS D L E+ +  LEM
Sbjct: 28  YAADLSSYEAACENDPDLQSFDVAVHERTTRVLNSLATGVEARSLSFDALMEVTDCLLEM 87

Query: 62  NQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAV 121
           NQ V KI+LE K+D+WSN EMF L N FFDNS+KT +FCT  ENCLRRTR+SQ IIKLAV
Sbjct: 88  NQDVVKIILESKEDVWSNKEMFKLVNEFFDNSLKTLDFCTGLENCLRRTRDSQFIIKLAV 147

Query: 122 KQFEEDQKGGDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRKL 181
           KQ EE + GGDE+ VRTFEELKKF+EAG+PFT EFVQL QSLY+QH S F KLQ EKR+L
Sbjct: 148 KQLEEVENGGDERYVRTFEELKKFQEAGDPFTLEFVQLFQSLYKQHLSMFKKLQTEKRRL 207

Query: 182 DKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWCN 241
           DKK NT K WKK SN+IFVTAF SV+IFS+VAA  SAPP+VIALAAA A+P+GPV KWCN
Sbjct: 208 DKKYNTTKTWKKVSNVIFVTAFASVLIFSVVAAAMSAPPVVIALAAALAVPMGPVGKWCN 267

Query: 242 TIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANLCIMEQG 301
           ++WNR+LK+I++EKQLI SMQ  T+ + K+ E IRVLV  LS +FG L   ANL I EQG
Sbjct: 268 SMWNRYLKKIKEEKQLISSMQGHTYIILKDFENIRVLVRKLSIQFGSLLQNANLGIREQG 327

Query: 302 AMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHP 344
           AMQLV++EIKKNL+GFDETIEKLSVHAG+CS+ VT    T +LQKI+RHP
Sbjct: 328 AMQLVIDEIKKNLEGFDETIEKLSVHAGKCSRDVT-MARTVILQKIVRHP 376

BLAST of MS023380 vs. ExPASy TrEMBL
Match: A0A6J1G6K3 (UPF0496 protein At2g18630-like OS=Cucurbita moschata OX=3662 GN=LOC111451340 PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 1.2e-101
Identity = 205/350 (58.57%), Postives = 254/350 (72.57%), Query Frame = 0

Query: 1   HYAANLSSHD-----GADLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLE 60
           HYAA LSS++       DLQ FD  V ERT   +NSLA  +G  SLS+D L EI E  LE
Sbjct: 29  HYAAALSSYETECENNPDLQSFDDKVHERTNRALNSLATGIGPSSLSLDVLMEIPEFLLE 88

Query: 61  MNQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLA 120
           MN+   K++L  K+D+WSN E+F L +AFFDNS+KT  FCT  ENCLRRTR+S+LIIK+A
Sbjct: 89  MNEDAVKLILASKEDVWSNKELFELVDAFFDNSLKTLHFCTALENCLRRTRDSELIIKVA 148

Query: 121 VKQFEEDQKGGDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRK 180
           + QFE  + G DE+ V+T EELKKF+E GNPF+ EFVQL   LY+QH S F KLQ +K+K
Sbjct: 149 INQFESGENGDDERYVKTIEELKKFQEVGNPFSQEFVQLFVPLYKQHLSMFKKLQHQKKK 208

Query: 181 LDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWC 240
           LDKK  TMK WKK +N+  VT F SV++FS+VAA  SAPP+VIAL AA  +P+GPV KWC
Sbjct: 209 LDKKYRTMKTWKKLTNVFLVTLFASVLVFSVVAAAMSAPPVVIALTAALVVPMGPVGKWC 268

Query: 241 NTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANLCIMEQ 300
           NT+WNR+L  I+ EKQLI S+Q  T+ V K+ E IR+LV  LS + G L    +L I EQ
Sbjct: 269 NTLWNRYLSHIKAEKQLINSVQGHTYIVLKDFENIRLLVQKLSIQLGSLLQNTDLGIREQ 328

Query: 301 GAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRH 343
           GAMQLV++EIKKNL+GFDETIEKLSVHA +CS  VT +  T ++QKI  H
Sbjct: 329 GAMQLVIDEIKKNLKGFDETIEKLSVHASKCSTDVT-KARTVIIQKIAMH 377

BLAST of MS023380 vs. ExPASy TrEMBL
Match: A0A6J1L3A2 (UPF0496 protein At2g18630-like OS=Cucurbita maxima OX=3661 GN=LOC111499512 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 3.3e-99
Identity = 199/350 (56.86%), Postives = 251/350 (71.71%), Query Frame = 0

Query: 1   HYAANLSSHD-----GADLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLE 60
           HY+A LSS++       DLQ FD  + ERT   ++SLA  +G  SLS+D L EI E  LE
Sbjct: 29  HYSAALSSYETECENNPDLQSFDAKLHERTNQALHSLATGIGPGSLSLDVLMEIPEFLLE 88

Query: 61  MNQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLA 120
           MN+   K++L  K+D+WSN E+F L +AFFDNS+KT  FC   ENCLRRTR+S+LIIK+A
Sbjct: 89  MNEDAVKLILASKEDVWSNKELFELVDAFFDNSLKTLHFCAALENCLRRTRDSELIIKVA 148

Query: 121 VKQFEEDQKGGDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRK 180
           + QFE  + G DE+ V+T E LKKF+E GNPF  EFVQL   LY+QH S F KLQ +K+K
Sbjct: 149 INQFESGENGDDERYVKTIEGLKKFQEVGNPFAQEFVQLFVPLYKQHLSMFKKLQHQKKK 208

Query: 181 LDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWC 240
           LDKK  TMK WKK +N+  VT F S+++FS+VAA  SAPP+VIAL AA  +P+GPV KWC
Sbjct: 209 LDKKYRTMKTWKKLTNVFLVTLFASILVFSVVAAAMSAPPVVIALTAALVVPMGPVGKWC 268

Query: 241 NTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANLCIMEQ 300
           NT+WNR+L  I+ EKQLI S+Q  T+ V K+ E IR+LV  LS + G L    +L I EQ
Sbjct: 269 NTLWNRYLSHIKAEKQLINSVQGHTYIVLKDFENIRLLVQKLSIQLGSLLQNTDLGIREQ 328

Query: 301 GAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRH 343
           GAMQLV++EIKKNL+GFDETIEKLSVHA +CS  VT +  T ++QKI  H
Sbjct: 329 GAMQLVIDEIKKNLKGFDETIEKLSVHASKCSTDVT-KARTVIIQKIAMH 377

BLAST of MS023380 vs. ExPASy TrEMBL
Match: A0A6J1EMX0 (UPF0496 protein At2g18630-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434982 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 2.1e-98
Identity = 199/337 (59.05%), Postives = 253/337 (75.07%), Query Frame = 0

Query: 13  DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEMNQSVAKIVLECKKDMW 72
           DLQ FDV VQERT+  +NSLA  V   SLS D L EI +  LEMN+   +I+LE K+D+W
Sbjct: 42  DLQSFDVRVQERTSRALNSLANRVQVGSLSFDALMEITDFLLEMNEDAVQIILESKEDVW 101

Query: 73  SNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAVKQFEEDQKGGD-EKCV 132
           SN E+F L + FF NS+KT +FC   E  LRR+R+SQ I+KLAV+QFE  + GGD E+ V
Sbjct: 102 SNKELFRLVDTFFHNSLKTLDFCAALEKSLRRSRDSQFIVKLAVRQFESYENGGDGERYV 161

Query: 133 RTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRKLDKKSNTMKAWKKASN 192
           RTFE+LKKF+EAG+PF  EF +L +SLY++H STFN LQ +K KLDKK ++MK WK+ SN
Sbjct: 162 RTFEQLKKFQEAGDPFGKEFGKLLKSLYDRHVSTFNDLQSQKTKLDKKFSSMKTWKRVSN 221

Query: 193 LIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWCNTIWNRHLKRIEKEKQ 252
            IF+TAF SV+IFS+VAA  SAPP+VIALAAA A+P+GPV KWCNT+WNR+L  I+ +KQ
Sbjct: 222 AIFITAFASVLIFSVVAAAMSAPPVVIALAAALAVPMGPVGKWCNTLWNRYLNSIKADKQ 281

Query: 253 LIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANLCIMEQGAMQLVVNEIKKNLQG 312
            + SM+  T+ + K+L+ I VLV  LS +FG L   A++ I E GAMQLV++EIKKNL G
Sbjct: 282 FMSSMEGHTYIILKDLQNIDVLVRRLSIQFGSLLQNADVGIRELGAMQLVIDEIKKNLDG 341

Query: 313 FDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHPAA 346
           FDETIEKLSVHA +CS  VT +  T +LQK+ RHP A
Sbjct: 342 FDETIEKLSVHATKCSSDVT-KARTVILQKMARHPTA 377

BLAST of MS023380 vs. TAIR 10
Match: AT2G18630.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 279.6 bits (714), Expect = 3.3e-75
Identity = 155/352 (44.03%), Postives = 227/352 (64.49%), Query Frame = 0

Query: 2   YAANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEM 61
           Y  +LSS++ A      L+ FD  + ERT  V+N LA  V   SLS D L E+ +  L+M
Sbjct: 30  YTEHLSSYERACSEDPKLESFDSALHERTNRVINKLASGVEIKSLSFDSLREVTQCLLDM 89

Query: 62  NQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAV 121
           NQ V K++L+ K+D+W+N ++F L N +F+++ KT +FC+  ENCL R R SQ+II+ AV
Sbjct: 90  NQDVVKVILQDKEDIWNNQDLFSLVNLYFESTAKTMDFCSELENCLNRARRSQVIIQFAV 149

Query: 122 KQFEEDQKGGD-EKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIEKRK 181
            QFEE+ +  +  K  +T EELK+F+ AG PFT EF  L   +Y+Q      +L   KRK
Sbjct: 150 NQFEEENEDKENRKYEKTLEELKRFKVAGEPFTKEFFALFDLVYKQQVMMLEELHKLKRK 209

Query: 182 LDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVAKWC 241
           LDK+   +K W++ SN++FVTAFVSV+IFS+VAA  +APP+V A+A A A+P+G V KWC
Sbjct: 210 LDKRLRNIKTWRRVSNMVFVTAFVSVLIFSVVAAAVAAPPVVAAIAGALAVPVGSVGKWC 269

Query: 242 NTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANLCIMEQ 301
           NT+W ++ K +  +K++I S++  T+   KE++ I +LV  +      L   A   I E+
Sbjct: 270 NTLWTKYEKVVRGQKEIITSIRIGTYISVKEMDNISILVRKVEVEIESLLKKAEFAITEE 329

Query: 302 GAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHPA 345
             ++L ++EIKK L  F ETIE+L  HAG+    VT +  T +LQ+IIR+PA
Sbjct: 330 KEVRLAIDEIKKKLDVFTETIEELGEHAGKYCSDVT-KARTVILQRIIRYPA 380

BLAST of MS023380 vs. TAIR 10
Match: AT4G34320.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 260.8 bits (665), Expect = 1.6e-69
Identity = 146/353 (41.36%), Postives = 223/353 (63.17%), Query Frame = 0

Query: 1   HYAANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLE 60
           HY   L S+  A     +LQ FD  +Q RT+ V+++LA  V   +LS D L E+ +  LE
Sbjct: 18  HYTTELRSYAAACKADTELQSFDTCLQARTSHVISTLATGVEVRALSFDSLKEVTQCLLE 77

Query: 61  MNQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLA 120
           MNQ V K++L+CKKD+W N EMF L   +F+NS+KT +FC   E  LRR R+S L+I +A
Sbjct: 78  MNQEVVKVILDCKKDIWKNQEMFELVEDYFENSLKTLDFCAALEKGLRRARDSHLLILVA 137

Query: 121 VKQFEED---QKGGDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQIE 180
           ++QFE++   Q G   K  +T EELK F++A +PF  +F ++ QS+Y+Q      KLQ  
Sbjct: 138 LQQFEDESLVQGGNGYK--KTLEELKNFKDAESPFNEDFFKMFQSVYKQQMLMLEKLQHR 197

Query: 181 KRKLDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGPVA 240
           K KLDKK   +  W+K S++IFV  F +V+I S+VAA  +APP+  ALAAA A+PLG + 
Sbjct: 198 KNKLDKKLKCIHTWRKLSSIIFVATFATVLICSVVAAAMAAPPVAAALAAATAVPLGSMG 257

Query: 241 KWCNTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRF-GLLANL-CIM 300
           KW +++W  +   ++ +K++I SMQ  TF   K+L+ IRVL+  L     G++ +    +
Sbjct: 258 KWIDSLWKNYENALKGQKEVISSMQAGTFVAVKDLDNIRVLIERLEIEITGMVKSAEFAV 317

Query: 301 EQGAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHP 344
           E  A+++ +++IKK L+ F + +E+L   A  CS+ +  +  T +LQ+II+HP
Sbjct: 318 EHNAVKIGIDDIKKKLEVFKKNVEELGTQADLCSRDI-RRARTVILQRIIKHP 367

BLAST of MS023380 vs. TAIR 10
Match: AT5G66675.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 256.1 bits (653), Expect = 3.9e-68
Identity = 145/362 (40.06%), Postives = 222/362 (61.33%), Query Frame = 0

Query: 2   YAANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEM 61
           Y+A+L++++ A     DLQ FD  + +RT  V+NSLA      SLS D L E+    LEM
Sbjct: 38  YSADLTAYNSACREDPDLQSFDSSLHQRTNRVINSLASGAQTRSLSFDALIEVSGCLLEM 97

Query: 62  NQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAV 121
           NQ V + ++E K+D+W N ++  L NA+FD+SIKT +FC   +NC++R R  Q++++ A+
Sbjct: 98  NQEVVRFIIESKEDVWDNKDLTCLVNAYFDSSIKTLDFCNAVDNCVKRARIGQMLLQFAL 157

Query: 122 KQFEEDQKG-------GDEKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKL 181
           KQFE +  G       G  K  +T EEL KF+ +G+PF  +F  L +S+YEQ       L
Sbjct: 158 KQFEMESSGSNKSVEPGTNKYAKTLEELNKFKASGDPFDGDFFMLLESVYEQQVMLLEIL 217

Query: 182 QIEKRKLDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLG 241
             +KRKLDKK   +K WKK SN++FVTAFVSV+IFS+VAA  +APP+V ALAAA A+P+G
Sbjct: 218 HKQKRKLDKKLKNIKYWKKISNVVFVTAFVSVLIFSVVAAAVAAPPVVTALAAALAVPIG 277

Query: 242 PVAKWCNTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLLANLCI 301
            + KWCN +W ++   ++ +K +++SM+   +   K+++ IRV V  L      +     
Sbjct: 278 SIGKWCNHLWKKYETAVKGQKDIVLSMRIGAYVTMKDMDNIRVHVDKLKIEMESMMQKVD 337

Query: 302 M------EQGAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKVTNQTTTALLQKIIRHP 346
                  E+ A++L ++EI K    F E IE++  +A +CSK +T    T +L+ I+  P
Sbjct: 338 FALKEKEEEVAVRLSMHEISKKFDVFTERIEEVGDNATKCSKNIT-LARTIVLRHILSFP 397

BLAST of MS023380 vs. TAIR 10
Match: AT5G66660.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 204.1 bits (518), Expect = 1.8e-52
Identity = 118/342 (34.50%), Postives = 199/342 (58.19%), Query Frame = 0

Query: 2   YAANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEM 61
           Y+++LSS+  A     +L+ FD  + +RT  ++ SLA      SL++D L E+    LE+
Sbjct: 38  YSSDLSSYTSACKKDSNLKSFDSSLHQRTNIIITSLAARAETQSLNLDSLMEVYGFLLEL 97

Query: 62  NQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAV 121
           NQ+  ++++E ++D+W N ++  L + +F ++ KT +FC   ENC++RT  SQLII+ AV
Sbjct: 98  NQNAVRVIIESREDVWKNKDLKSLVDVYFKSTSKTLDFCNTVENCVKRTEISQLIIRFAV 157

Query: 122 KQFE----EDQKGGD---EKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKL 181
           KQFE    +   GGD   +K  +T EEL KF+  G+PF  E V    S+Y+Q      +L
Sbjct: 158 KQFEAESVDTDLGGDKKKKKYTKTLEELNKFKAMGDPFDGELVTQFDSVYDQQVLFLEEL 217

Query: 182 QIEKRKLDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLG 241
           + ++RKLDKK   +K  +  SN+ F TA+VSV++ S+VA   SAPP+V A+A+    P+ 
Sbjct: 218 RKQRRKLDKKQRNVKTLRTVSNVFFATAYVSVLVLSVVATTMSAPPVVCAVASGSTAPIE 277

Query: 242 PVAKWCNTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLLANLCI 301
              KW + +W ++ K +++++ L+++M+       + ++ IR  V  L +    +     
Sbjct: 278 ITGKWFSQMWKKYEKAVKRQRGLVLTMESRVQVNNEAMKNIRSDVDELRSWVSSILETVD 337

Query: 302 M------EQGAMQLVVNEIKKNLQGFDETIEKLSVHAGECSK 326
                  E+ AM L +  IKK++ GF E +E++  +A +CSK
Sbjct: 338 FAVEREEEEEAMGLAMQGIKKHVDGFTEKMEEVGENAAKCSK 379

BLAST of MS023380 vs. TAIR 10
Match: AT5G66670.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 199.5 bits (506), Expect = 4.3e-51
Identity = 116/343 (33.82%), Postives = 198/343 (57.73%), Query Frame = 0

Query: 2   YAANLSSHDGA-----DLQFFDVVVQERTTGVVNSLAMEVGAWSLSMDELNEIIEHPLEM 61
           Y+++LSS+  A      L+ FD ++ ERT  +++SLA +    SL+++ L E+  + LE+
Sbjct: 38  YSSDLSSYASACKKYSSLKSFDSLLHERTNSIISSLAAQAKTRSLNIESLMEVYGYLLEL 97

Query: 62  NQSVAKIVLECKKDMWSNMEMFHLSNAFFDNSIKTSEFCTVSENCLRRTRNSQLIIKLAV 121
           NQ   ++++E K+D+  N ++  L + +F ++ KT +FC   E C+++   SQLII+ AV
Sbjct: 98  NQDTVRVIIESKEDVLKNNDLKALVDVYFKSTSKTLDFCNTVEKCVKKAEISQLIIRFAV 157

Query: 122 KQFEEDQKGGD------EKCVRTFEELKKFEEAGNPFTPEFVQLAQSLYEQHCSTFNKLQ 181
           KQFE +    D      +K V+T EE+ KF+  G+PF  EFV   +S+Y++     ++L+
Sbjct: 158 KQFETETVDTDLGESKKKKYVKTLEEMNKFKAMGDPFDGEFVTQYKSVYDEQVLLLDELR 217

Query: 182 IEKRKLDKKSNTMKAWKKASNLIFVTAFVSVVIFSIVAAVKSAPPLVIALAAAQAIPLGP 241
             K KL KK   +K W+  SN++F TAFV+V + S+VAA   APP++ A+A+    P+  
Sbjct: 218 KLKVKLGKKLRNIKTWRILSNVVFATAFVTVFVLSVVAAAMMAPPVLSAVASGLTTPIEV 277

Query: 242 VAKWCNTIWNRHLKRIEKEKQLIISMQRPTFAVTKELEEIRVLVPILSNRFGLL---ANL 301
           V  WCN +W  + K +++++ L+++M+    A    +  I+  V  LS R   +    N 
Sbjct: 278 VGMWCNKMWKEYEKAVKRQRGLVLTMELGVQANNVTMVNIKFEVENLSIRISSILKTVNF 337

Query: 302 CI---MEQGAMQLVVNEIKKNLQGFDETIEKLSVHAGECSKKV 328
            +     + A +  + EIKK ++GF E IE++   A  CSK +
Sbjct: 338 AVDREENEMATRFAMQEIKKKVEGFTEKIEEVGERAANCSKLI 380