MS023179 (gene) Bitter gourd (TR) v1

BLAST of MS023179 vs. NCBI nr
Match: XP_022147692.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Momordica charantia])

HSP 1 Score: 708.8 bits (1828), Expect = 2.4e-200
Identity = 343/346 (99.13%), Postives = 345/346 (99.71%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFG 60
           MVTILRRSQTVLNSICRRKFSSSSDGDLTKP+GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEP YMPTKNK+
Sbjct: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPXYMPTKNKI 346

BLAST of MS023179 vs. NCBI nr
Match: XP_023515905.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 689.9 bits (1779), Expect = 1.2e-194
Identity = 331/346 (95.66%), Postives = 341/346 (98.55%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFG 60
           MV+ILRRS  +LNSICRR+FS+SSDG+LT   GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLA+RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           FGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEPFYMPTKNK+
Sbjct: 301 FGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKI 346

BLAST of MS023179 vs. NCBI nr
Match: XP_022960762.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita moschata] >KAG6590071.1 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 688.3 bits (1775), Expect = 3.4e-194
Identity = 330/346 (95.38%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFG 60
           MV+ILRRS  +LNSICRR+FS+SSDG+LT   GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGL +RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           FGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEPFYMPTKNK+
Sbjct: 301 FGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKI 346

BLAST of MS023179 vs. NCBI nr
Match: XP_008450996.1 (PREDICTED: 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucumis melo])

HSP 1 Score: 680.2 bits (1754), Expect = 9.3e-192
Identity = 324/346 (93.64%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFG 60
           M ++LRRSQTVLNS+CRR+FS+S DG+LT   GKSMNLYSAINQALHIALETDPR+YVFG
Sbjct: 1   MASVLRRSQTVLNSLCRRQFSASPDGNLTTHAGKSMNLYSAINQALHIALETDPRAYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLA+RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRD+NPV+FFEPKWLYRLAVEEVPE DFMLPLS+AEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDENPVIFFEPKWLYRLAVEEVPEDDFMLPLSQAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQAC+DAEK+GISCELIDLRTLLPWDKETVEASV+KTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACVDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNK+
Sbjct: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKI 346

BLAST of MS023179 vs. NCBI nr
Match: XP_004144063.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucumis sativus] >KAE8653468.1 hypothetical protein Csa_007124 [Cucumis sativus])

HSP 1 Score: 679.9 bits (1753), Expect = 1.2e-191
Identity = 325/346 (93.93%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFG 60
           M ++LRRSQTVLNS+CRR+FS+S DG+LT   GKSMNLYSAINQALHIALETDPR+YVFG
Sbjct: 1   MASVLRRSQTVLNSLCRRQFSASPDGNLTTHAGKSMNLYSAINQALHIALETDPRAYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLA+RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRD+NPVVFFEPKWLYRLAVEEVPE DFMLPLS+A+VIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDENPVVFFEPKWLYRLAVEEVPEDDFMLPLSQADVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEK+GISCELIDLRTLLPWDKETVEASV+KTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNK+
Sbjct: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKI 346

BLAST of MS023179 vs. ExPASy Swiss-Prot
Match: Q9SAV3 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCDH BETA1 PE=2 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 1.2e-178
Identity = 300/339 (88.50%), Postives = 320/339 (94.40%), Query Frame = 0

Query: 11  VLNSICRR-KFSSSSDG--DLTKPNGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGG 70
           +L   CR+  F S S G   ++   GK +NLYSAINQALHIAL+TDPRSYVFGEDVGFGG
Sbjct: 4   LLGRSCRKLSFPSLSHGARRVSTETGKPLNLYSAINQALHIALDTDPRSYVFGEDVGFGG 63

Query: 71  VFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVN 130
           VFRCTTGLAER+GK+RVFNTPLCEQGIVGFGIGLAAMGNRAI EIQFADYIYPAFDQIVN
Sbjct: 64  VFRCTTGLAERFGKNRVFNTPLCEQGIVGFGIGLAAMGNRAIVEIQFADYIYPAFDQIVN 123

Query: 131 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAK 190
           EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSP +AK
Sbjct: 124 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAK 183

Query: 191 GLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLS 250
           GLLLSCIRD NPVVFFEPKWLYR AVEEVPEHD+M+PLSEAEVIREGNDITLVGWGAQL+
Sbjct: 184 GLLLSCIRDPNPVVFFEPKWLYRQAVEEVPEHDYMIPLSEAEVIREGNDITLVGWGAQLT 243

Query: 251 VMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 310
           VMEQAC+DAEK+GISCELIDL+TLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA
Sbjct: 244 VMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 303

Query: 311 SIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           +I+ERCFL+LEAP+SRVCGLDTPFPLVFEPFYMPTKNK+
Sbjct: 304 TILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKI 342

BLAST of MS023179 vs. ExPASy Swiss-Prot
Match: Q9LDY2 (2-oxoisovalerate dehydrogenase subunit beta 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DIN4 PE=1 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 3.4e-173
Identity = 285/315 (90.48%), Postives = 305/315 (96.83%), Query Frame = 0

Query: 32  NGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCE 91
           +GKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAER+GK RVFNTPLCE
Sbjct: 34  SGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERFGKSRVFNTPLCE 93

Query: 92  QGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY 151
           QGIVGFGIGLAAMGNR IAEIQFADYI+PAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY
Sbjct: 94  QGIVGFGIGLAAMGNRVIAEIQFADYIFPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY 153

Query: 152 GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRL 211
           GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSP +AKGLLLS IRD NPVVFFEPKWLYR 
Sbjct: 154 GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAKGLLLSSIRDPNPVVFFEPKWLYRQ 213

Query: 212 AVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVMEQACIDAEKDGISCELIDLRTL 271
           AVE+VPE D+M+PLSEAEV+REG+DITLVGWGAQL++MEQAC+DAE +GISCELIDL+TL
Sbjct: 214 AVEDVPEDDYMIPLSEAEVMREGSDITLVGWGAQLTIMEQACLDAENEGISCELIDLKTL 273

Query: 272 LPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPF 331
           +PWDKE VE SV+KTGRLLISHEAPVTGGFGAEI+A+IVERCFLRLEAP+SRVCGLDTPF
Sbjct: 274 IPWDKEIVETSVRKTGRLLISHEAPVTGGFGAEIAATIVERCFLRLEAPVSRVCGLDTPF 333

Query: 332 PLVFEPFYMPTKNKV 347
           PLVFEPFYMPTKNK+
Sbjct: 334 PLVFEPFYMPTKNKI 348

BLAST of MS023179 vs. ExPASy Swiss-Prot
Match: P21839 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Bos taurus OX=9913 GN=BCKDHB PE=1 SV=2)

HSP 1 Score: 471.5 bits (1212), Expect = 8.6e-132
Identity = 220/313 (70.29%), Postives = 260/313 (83.07%), Query Frame = 0

Query: 34  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQG 93
           + MNL+ A+  AL  +L  DP + +FGEDV FGGVFRCT GL ++YGKDRVFNTPLCEQG
Sbjct: 69  QKMNLFQAVTSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQG 128

Query: 94  IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 153
           IVGFGIG+A  G  AIAEIQFADYI+PAFDQIVNEAAK+RYRSG+ FNCG LTIR+P+G 
Sbjct: 129 IVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRSPWGC 188

Query: 154 VGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAV 213
           VGHG  YHSQSPEAFF H PGIKVV+PRSP+QAKGLLLSCI D+NP +FFEPK LYR AV
Sbjct: 189 VGHGALYHSQSPEAFFAHCPGIKVVVPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAV 248

Query: 214 EEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVM-EQACIDAEKDGISCELIDLRTLL 273
           E+VP   + +PLS+AEVI+EG+D+TLV WG Q+ V+ E A +  EK G+SCE+IDLRT+L
Sbjct: 249 EQVPVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVAAMAQEKLGVSCEVIDLRTIL 308

Query: 274 PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPFP 333
           PWD +TV  SV KTGRLL+SHEAP+TGGF +EIS+++ E CFL LEAPISRVCG DTPFP
Sbjct: 309 PWDVDTVCKSVIKTGRLLVSHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFP 368

Query: 334 LVFEPFYMPTKNK 346
            +FEPFY+P K K
Sbjct: 369 HIFEPFYIPDKWK 381

BLAST of MS023179 vs. ExPASy Swiss-Prot
Match: Q6P3A8 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Mus musculus OX=10090 GN=Bckdhb PE=1 SV=2)

HSP 1 Score: 471.5 bits (1212), Expect = 8.6e-132
Identity = 222/313 (70.93%), Postives = 260/313 (83.07%), Query Frame = 0

Query: 34  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQG 93
           + MNL+ +I  AL  +L  DP + +FGEDV FGGVFRCT GL ++YGKDRVFNTPLCEQG
Sbjct: 67  QKMNLFQSITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQG 126

Query: 94  IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 153
           IVGFGIG+A  G  AIAEIQFADYI+PAFDQIVNEAAK+RYRSG+ FNCG LTIRAP+G 
Sbjct: 127 IVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGC 186

Query: 154 VGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAV 213
           VGHG  YHSQSPEAFF H PGIKVVIPRSP+QAKGLLLSCI D+NP +FFEPK LYR AV
Sbjct: 187 VGHGALYHSQSPEAFFAHCPGIKVVIPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAV 246

Query: 214 EEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVM-EQACIDAEKDGISCELIDLRTLL 273
           E+VP   + +PLS+AEVI+EG+D+TLV WG Q+ V+ E A +  EK G+SCE+IDLRT++
Sbjct: 247 EQVPVEPYKIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTIV 306

Query: 274 PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPFP 333
           PWD +TV  SV KTGRLLISHEAP+TGGF +EIS+++ E CFL LEAPISRVCG DTPFP
Sbjct: 307 PWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFP 366

Query: 334 LVFEPFYMPTKNK 346
            +FEPFY+P K K
Sbjct: 367 HIFEPFYIPDKWK 379

BLAST of MS023179 vs. ExPASy Swiss-Prot
Match: P35738 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Rattus norvegicus OX=10116 GN=Bckdhb PE=1 SV=3)

HSP 1 Score: 471.5 bits (1212), Expect = 8.6e-132
Identity = 222/313 (70.93%), Postives = 260/313 (83.07%), Query Frame = 0

Query: 34  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQG 93
           + MNL+ +I  AL  +L  DP + +FGEDV FGGVFRCT GL ++YGKDRVFNTPLCEQG
Sbjct: 67  QKMNLFQSITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQG 126

Query: 94  IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 153
           IVGFGIG+A  G  AIAEIQFADYI+PAFDQIVNEAAK+RYRSG+ FNCG LTIRAP+G 
Sbjct: 127 IVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGC 186

Query: 154 VGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAV 213
           VGHG  YHSQSPEAFF H PGIKVVIPRSP+QAKGLLLSCI D+NP +FFEPK LYR AV
Sbjct: 187 VGHGALYHSQSPEAFFAHCPGIKVVIPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAV 246

Query: 214 EEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVM-EQACIDAEKDGISCELIDLRTLL 273
           E+VP   + +PLS+AEVI+EG+D+TLV WG Q+ V+ E A +  EK G+SCE+IDLRT++
Sbjct: 247 EQVPVEPYKIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTIV 306

Query: 274 PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPFP 333
           PWD +TV  SV KTGRLLISHEAP+TGGF +EIS+++ E CFL LEAPISRVCG DTPFP
Sbjct: 307 PWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFP 366

Query: 334 LVFEPFYMPTKNK 346
            +FEPFY+P K K
Sbjct: 367 HIFEPFYIPDKWK 379

BLAST of MS023179 vs. ExPASy TrEMBL
Match: A0A6J1D329 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016561 PE=4 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.2e-200
Identity = 343/346 (99.13%), Postives = 345/346 (99.71%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFG 60
           MVTILRRSQTVLNSICRRKFSSSSDGDLTKP+GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEP YMPTKNK+
Sbjct: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPXYMPTKNKI 346

BLAST of MS023179 vs. ExPASy TrEMBL
Match: A0A6J1H8B9 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461465 PE=4 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 1.7e-194
Identity = 330/346 (95.38%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFG 60
           MV+ILRRS  +LNSICRR+FS+SSDG+LT   GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGL +RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           FGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEPFYMPTKNK+
Sbjct: 301 FGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKI 346

BLAST of MS023179 vs. ExPASy TrEMBL
Match: A0A1S3BQK1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103492412 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 4.5e-192
Identity = 324/346 (93.64%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFG 60
           M ++LRRSQTVLNS+CRR+FS+S DG+LT   GKSMNLYSAINQALHIALETDPR+YVFG
Sbjct: 1   MASVLRRSQTVLNSLCRRQFSASPDGNLTTHAGKSMNLYSAINQALHIALETDPRAYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLA+RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRD+NPV+FFEPKWLYRLAVEEVPE DFMLPLS+AEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDENPVIFFEPKWLYRLAVEEVPEDDFMLPLSQAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQAC+DAEK+GISCELIDLRTLLPWDKETVEASV+KTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACVDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNK+
Sbjct: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKI 346

BLAST of MS023179 vs. ExPASy TrEMBL
Match: A0A6J1JGI1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484908 PE=4 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 2.9e-191
Identity = 327/346 (94.51%), Postives = 338/346 (97.69%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFG 60
           MV+ILRRS  +L+SICRR+FS+SSDG+LT   GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLSSICRRQFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGL +RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPK LYRLAVEEVPE DFMLPLSEAEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKLLYRLAVEEVPEDDFMLPLSEAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLMPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           FGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEPFYMPTKNK+
Sbjct: 301 FGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKI 346

BLAST of MS023179 vs. ExPASy TrEMBL
Match: A0A660KMJ8 (Transket_pyr domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_009543 PE=4 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 5.2e-180
Identity = 303/341 (88.86%), Postives = 326/341 (95.60%), Query Frame = 0

Query: 9   QTVLNSIC---RRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 68
           +T+  SIC   RR FS+S D  L +P+ KS NLYSAINQALHIALETDPR+YVFGEDVGF
Sbjct: 7   RTIGRSICRVSRRGFSTSCDDKLVQPSSKSTNLYSAINQALHIALETDPRAYVFGEDVGF 66

Query: 69  GGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 128
           GGVFRCTTGLA+R+GK RVFNTPLCEQGI+GFGIGLAAMGNRA+AEIQFADYIYPAFDQI
Sbjct: 67  GGVFRCTTGLADRFGKSRVFNTPLCEQGIIGFGIGLAAMGNRAVAEIQFADYIYPAFDQI 126

Query: 129 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQ 188
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSP Q
Sbjct: 127 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPQQ 186

Query: 189 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQ 248
           AKGLLLSCIRD NPVVFFEPKWLYRLAVEEVPE+D+MLPLSEAEVIREG+DITLVGWGAQ
Sbjct: 187 AKGLLLSCIRDPNPVVFFEPKWLYRLAVEEVPEYDYMLPLSEAEVIREGSDITLVGWGAQ 246

Query: 249 LSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 308
           LSVMEQAC+DAEK+GISCELIDL+TL+PWDKET+E+SV+KTGRLLISHEAPVTGGFGAEI
Sbjct: 247 LSVMEQACLDAEKEGISCELIDLKTLIPWDKETIESSVRKTGRLLISHEAPVTGGFGAEI 306

Query: 309 SASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           SASIVERCFLRLEAP++RVCGLDTPFPLVFEPFYMPTKNK+
Sbjct: 307 SASIVERCFLRLEAPVARVCGLDTPFPLVFEPFYMPTKNKI 347

BLAST of MS023179 vs. TAIR 10
Match: AT1G55510.1 (branched-chain alpha-keto acid decarboxylase E1 beta subunit )

HSP 1 Score: 627.1 bits (1616), Expect = 8.7e-180
Identity = 300/339 (88.50%), Postives = 320/339 (94.40%), Query Frame = 0

Query: 11  VLNSICRR-KFSSSSDG--DLTKPNGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGG 70
           +L   CR+  F S S G   ++   GK +NLYSAINQALHIAL+TDPRSYVFGEDVGFGG
Sbjct: 4   LLGRSCRKLSFPSLSHGARRVSTETGKPLNLYSAINQALHIALDTDPRSYVFGEDVGFGG 63

Query: 71  VFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVN 130
           VFRCTTGLAER+GK+RVFNTPLCEQGIVGFGIGLAAMGNRAI EIQFADYIYPAFDQIVN
Sbjct: 64  VFRCTTGLAERFGKNRVFNTPLCEQGIVGFGIGLAAMGNRAIVEIQFADYIYPAFDQIVN 123

Query: 131 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAK 190
           EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSP +AK
Sbjct: 124 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAK 183

Query: 191 GLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLS 250
           GLLLSCIRD NPVVFFEPKWLYR AVEEVPEHD+M+PLSEAEVIREGNDITLVGWGAQL+
Sbjct: 184 GLLLSCIRDPNPVVFFEPKWLYRQAVEEVPEHDYMIPLSEAEVIREGNDITLVGWGAQLT 243

Query: 251 VMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 310
           VMEQAC+DAEK+GISCELIDL+TLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA
Sbjct: 244 VMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 303

Query: 311 SIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKV 347
           +I+ERCFL+LEAP+SRVCGLDTPFPLVFEPFYMPTKNK+
Sbjct: 304 TILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKI 342

BLAST of MS023179 vs. TAIR 10
Match: AT3G13450.1 (Transketolase family protein )

HSP 1 Score: 609.0 bits (1569), Expect = 2.4e-174
Identity = 285/315 (90.48%), Postives = 305/315 (96.83%), Query Frame = 0

Query: 32  NGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCE 91
           +GKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAER+GK RVFNTPLCE
Sbjct: 34  SGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERFGKSRVFNTPLCE 93

Query: 92  QGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY 151
           QGIVGFGIGLAAMGNR IAEIQFADYI+PAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY
Sbjct: 94  QGIVGFGIGLAAMGNRVIAEIQFADYIFPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY 153

Query: 152 GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRL 211
           GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSP +AKGLLLS IRD NPVVFFEPKWLYR 
Sbjct: 154 GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAKGLLLSSIRDPNPVVFFEPKWLYRQ 213

Query: 212 AVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVMEQACIDAEKDGISCELIDLRTL 271
           AVE+VPE D+M+PLSEAEV+REG+DITLVGWGAQL++MEQAC+DAE +GISCELIDL+TL
Sbjct: 214 AVEDVPEDDYMIPLSEAEVMREGSDITLVGWGAQLTIMEQACLDAENEGISCELIDLKTL 273

Query: 272 LPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPF 331
           +PWDKE VE SV+KTGRLLISHEAPVTGGFGAEI+A+IVERCFLRLEAP+SRVCGLDTPF
Sbjct: 274 IPWDKEIVETSVRKTGRLLISHEAPVTGGFGAEIAATIVERCFLRLEAPVSRVCGLDTPF 333

Query: 332 PLVFEPFYMPTKNKV 347
           PLVFEPFYMPTKNK+
Sbjct: 334 PLVFEPFYMPTKNKI 348

BLAST of MS023179 vs. TAIR 10
Match: AT5G50850.1 (Transketolase family protein )

HSP 1 Score: 228.0 bits (580), Expect = 1.2e-59
Identity = 127/336 (37.80%), Postives = 185/336 (55.06%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFG 60
           M+ ILR+      S  RR   +           K M +  A+N A+   +  DP+ +V G
Sbjct: 1   MLGILRQRAIDGASTLRRTRFALVSARSYAAGAKEMTVRDALNSAIDEEMSADPKVFVMG 60

Query: 61  EDVG-FGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIY 120
           E+VG + G ++ T GL E+YG +RV++TP+ E G  G G+G A  G + + E    ++  
Sbjct: 61  EEVGQYQGAYKITKGLLEKYGPERVYDTPITEAGFTGIGVGAAYAGLKPVVEFMTFNFSM 120

Query: 121 PAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVI 180
            A D I+N AAK  Y S  Q N   +  R P GA    G  HSQ   A++  VPG+KV+ 
Sbjct: 121 QAIDHIINSAAKSNYMSAGQINV-PIVFRGPNGAAAGVGAQHSQCYAAWYASVPGLKVLA 180

Query: 181 PRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAV---EEVPEHDFMLPLSEAEVIREGND 240
           P S   A+GLL + IRD +PVVF E + LY  +    EE  +  F LP+ +A++ REG D
Sbjct: 181 PYSAEDARGLLKAAIRDPDPVVFLENELLYGESFPISEEALDSSFCLPIGKAKIEREGKD 240

Query: 241 ITLVGWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAP 300
           +T+V +   +    +A     ++GIS E+I+LR++ P D+ T+ ASV+KT RL+   E  
Sbjct: 241 VTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGF 300

Query: 301 VTGGFGAEISASIVERCFLRLEAPISRVCGLDTPFP 333
              G  AEI AS+VE  F  L+AP+ R+ G D P P
Sbjct: 301 PQHGVCAEICASVVEESFSYLDAPVERIAGADVPMP 335

BLAST of MS023179 vs. TAIR 10
Match: AT2G34590.1 (Transketolase family protein )

HSP 1 Score: 219.5 bits (558), Expect = 4.2e-57
Identity = 123/330 (37.27%), Postives = 184/330 (55.76%), Query Frame = 0

Query: 4   ILRRSQTVLNSICRRKFSSSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFGEDV 63
           + RRS+ ++ +    K  +++    +KP G  + L+ A+ + L   ++ DP   V GEDV
Sbjct: 55  VARRSEPLIPNAVTTKADTAASSTSSKP-GHELLLFEALQEGLEEEMDRDPHVCVMGEDV 114

Query: 64  G-FGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAF 123
           G +GG ++ T GLA+++G  RV +TP+CE    G GIG A  G R + E     ++  AF
Sbjct: 115 GHYGGSYKVTKGLADKFGDLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAF 174

Query: 124 DQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRS 183
           +QI N      Y SG QF    + IR P G     G  HSQ  E++F  +PGI++V   +
Sbjct: 175 NQISNNCGMLHYTSGGQFTI-PVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACST 234

Query: 184 PYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGW 243
           PY AKGL+ + IR +NPV+ FE   LY L  E +P+ +++  L EAE++R G  IT++ +
Sbjct: 235 PYNAKGLMKAAIRSENPVILFEHVLLYNLK-ESIPDEEYICNLEEAEMVRPGEHITILTY 294

Query: 244 GAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFG 303
                 + QA       G   E+ID+R+L P+D  T+  SVKKT R+LI  E   TGG G
Sbjct: 295 SRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLYTIGNSVKKTHRVLIVEECMRTGGIG 354

Query: 304 AEISASIVERCFLRLEAPISRVCGLDTPFP 333
           A ++A+I E     L+AP+  +   D P P
Sbjct: 355 ASLTAAINENFHDYLDAPVMCLSSQDVPTP 381

BLAST of MS023179 vs. TAIR 10
Match: AT1G30120.1 (pyruvate dehydrogenase E1 beta )

HSP 1 Score: 218.8 bits (556), Expect = 7.1e-57
Identity = 119/312 (38.14%), Postives = 175/312 (56.09%), Query Frame = 0

Query: 22  SSSDGDLTKPNGKSMNLYSAINQALHIALETDPRSYVFGEDVG-FGGVFRCTTGLAERYG 81
           ++ + D +   G  + L+ A+ + L   ++ DP   V GEDVG +GG ++ T GLA+++G
Sbjct: 72  ATKEADTSASTGHELLLFEALQEGLEEEMDRDPHVCVMGEDVGHYGGSYKVTKGLADKFG 131

Query: 82  KDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQF 141
             RV +TP+CE    G GIG A  G R + E     ++  AF+QI N      Y SG QF
Sbjct: 132 DLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQISNNCGMLHYTSGGQF 191

Query: 142 NCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPV 201
               + IR P G     G  HSQ  E++F  +PGI++V   +PY AKGL+ + IR +NPV
Sbjct: 192 TI-PVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAAIRSENPV 251

Query: 202 VFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVMEQACIDAEKDG 261
           + FE   LY L  E++P+ D++  L EAE++R G  IT++ +      + QA       G
Sbjct: 252 ILFEHVLLYNLK-EKIPDEDYVCNLEEAEMVRPGEHITILTYSRMRYHVMQAAKTLVNKG 311

Query: 262 ISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAP 321
              E+ID+R+L P+D  T+  SVKKT R+LI  E   TGG GA ++A+I E     L+AP
Sbjct: 312 YDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAAINENFHDYLDAP 371

Query: 322 ISRVCGLDTPFP 333
           +  +   D P P
Sbjct: 372 VMCLSSQDVPTP 381