MS023168 (gene) Bitter gourd (TR) v1

Overview
NameMS023168
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionReverse transcriptase domain-containing protein
Locationscaffold78: 633919 .. 634161 (+)
RNA-Seq ExpressionMS023168
SyntenyMS023168
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGCGGCTTCTCTCTGACCCGTCTTCTCTCCTTGCCCGAGTTCTGAAGTTCAAATATTTTAAGAATGGTGATTTTCTGAGTGCTAGGGCAGGGTCCAATGCTTCTTTCATATGGCGGAGTCTCTTTCAGGGTCGGGATCTTCTTCGTGCTGGTCCTTGGTGGAGAGTGGGCAACGGTAAGTCCATTAGAGTTTATTCTGATAATTGGATTCCCATGGATGGGTCTTTGTCCATTCGCTCACCG

mRNA sequence

TGGCGGCTTCTCTCTGACCCGTCTTCTCTCCTTGCCCGAGTTCTGAAGTTCAAATATTTTAAGAATGGTGATTTTCTGAGTGCTAGGGCAGGGTCCAATGCTTCTTTCATATGGCGGAGTCTCTTTCAGGGTCGGGATCTTCTTCGTGCTGGTCCTTGGTGGAGAGTGGGCAACGGTAAGTCCATTAGAGTTTATTCTGATAATTGGATTCCCATGGATGGGTCTTTGTCCATTCGCTCACCG

Coding sequence (CDS)

TGGCGGCTTCTCTCTGACCCGTCTTCTCTCCTTGCCCGAGTTCTGAAGTTCAAATATTTTAAGAATGGTGATTTTCTGAGTGCTAGGGCAGGGTCCAATGCTTCTTTCATATGGCGGAGTCTCTTTCAGGGTCGGGATCTTCTTCGTGCTGGTCCTTGGTGGAGAGTGGGCAACGGTAAGTCCATTAGAGTTTATTCTGATAATTGGATTCCCATGGATGGGTCTTTGTCCATTCGCTCACCG

Protein sequence

WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGKSIRVYSDNWIPMDGSLSIRSP
Homology
BLAST of MS023168 vs. NCBI nr
Match: XP_022159057.1 (uncharacterized protein LOC111025497 [Momordica charantia])

HSP 1 Score: 122.1 bits (305), Expect = 2.2e-24
Identity = 56/80 (70.00%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           W++ SDPSSLLA VLKFKYFKN DF+ A  GS ASFIWRSL  G DLLR G  WRVGNG 
Sbjct: 320 WQIFSDPSSLLAWVLKFKYFKNCDFIEASVGSRASFIWRSLLWGCDLLRIGLRWRVGNGA 379

Query: 61  SIRVYSDNWIPMDGSLSIRS 81
           +IR+Y DNWIP DG LS++S
Sbjct: 380 NIRIYEDNWIPRDGILSVQS 399

BLAST of MS023168 vs. NCBI nr
Match: XP_022149385.1 (uncharacterized protein LOC111017816 [Momordica charantia])

HSP 1 Score: 105.1 bits (261), Expect = 2.8e-19
Identity = 45/81 (55.56%), Postives = 59/81 (72.84%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           WR++ D SSLLA  LK +YFKN  FL A  GSN S+IWRSL +GR +L++G  WRVG+G 
Sbjct: 384 WRIMRDSSSLLALALKGRYFKNKTFLDADVGSNPSYIWRSLLRGRSILQSGLRWRVGDGL 443

Query: 61  SIRVYSDNWIPMDGSLSIRSP 82
           ++ +Y  NWIP DG+L + SP
Sbjct: 444 NVPIYGSNWIPRDGNLHVHSP 464

BLAST of MS023168 vs. NCBI nr
Match: XP_042944517.1 (uncharacterized protein LOC122278389 [Carya illinoinensis])

HSP 1 Score: 103.6 bits (257), Expect = 8.2e-19
Identity = 43/81 (53.09%), Postives = 57/81 (70.37%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           WR++  PSSL ARVLK KYF   DFL A+ GSNASF+WRS  + R +L+ G  WR+GNG 
Sbjct: 769 WRIIQHPSSLAARVLKAKYFFRTDFLKAKVGSNASFLWRSFMEARPVLQEGLTWRIGNGD 828

Query: 61  SIRVYSDNWIPMDGSLSIRSP 82
           S+ +++D WIP   S  ++SP
Sbjct: 829 SVHIWADKWIPRPSSYKVQSP 849

BLAST of MS023168 vs. NCBI nr
Match: KAF5458995.1 (hypothetical protein F2P56_022987 [Juglans regia])

HSP 1 Score: 101.7 bits (252), Expect = 3.1e-18
Identity = 45/81 (55.56%), Postives = 57/81 (70.37%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           WR+LS+PSSL+A+VLK KYF+N D L AR     S IWRS+    DLL+ G  WRVGNGK
Sbjct: 568 WRILSNPSSLVAKVLKEKYFRNSDLLEARLEYRPSLIWRSIRGALDLLKDGMVWRVGNGK 627

Query: 61  SIRVYSDNWIPMDGSLSIRSP 82
           S+R++ D W+P   S  I+SP
Sbjct: 628 SVRIWGDRWVPKPSSFCIQSP 648

BLAST of MS023168 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 100.9 bits (250), Expect = 5.3e-18
Identity = 40/71 (56.34%), Postives = 57/71 (80.28%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           WRLL  P+SL++RVL+ +YF+N  FL A+AG+NAS+IWRS+  GR +++ G  WR+GNGK
Sbjct: 906 WRLLQYPNSLVSRVLQARYFRNSSFLCAKAGANASYIWRSIMWGRQVIKKGMRWRIGNGK 965

Query: 61  SIRVYSDNWIP 72
            I ++SDNW+P
Sbjct: 966 KIAIFSDNWLP 976

BLAST of MS023168 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.0e-08
Identity = 24/70 (34.29%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           +R++  P +LL+R+L+ +YF +   +    G+  S+ WRS+  GR+LL  G    +G+G 
Sbjct: 75  FRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGI 134

Query: 61  SIRVYSDNWI 71
             +V+ D WI
Sbjct: 135 HTKVWLDRWI 144

BLAST of MS023168 vs. ExPASy TrEMBL
Match: A0A6J1DYS7 (uncharacterized protein LOC111025497 OS=Momordica charantia OX=3673 GN=LOC111025497 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-24
Identity = 56/80 (70.00%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           W++ SDPSSLLA VLKFKYFKN DF+ A  GS ASFIWRSL  G DLLR G  WRVGNG 
Sbjct: 320 WQIFSDPSSLLAWVLKFKYFKNCDFIEASVGSRASFIWRSLLWGCDLLRIGLRWRVGNGA 379

Query: 61  SIRVYSDNWIPMDGSLSIRS 81
           +IR+Y DNWIP DG LS++S
Sbjct: 380 NIRIYEDNWIPRDGILSVQS 399

BLAST of MS023168 vs. ExPASy TrEMBL
Match: A0A6J1D5K1 (uncharacterized protein LOC111017816 OS=Momordica charantia OX=3673 GN=LOC111017816 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.4e-19
Identity = 45/81 (55.56%), Postives = 59/81 (72.84%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           WR++ D SSLLA  LK +YFKN  FL A  GSN S+IWRSL +GR +L++G  WRVG+G 
Sbjct: 384 WRIMRDSSSLLALALKGRYFKNKTFLDADVGSNPSYIWRSLLRGRSILQSGLRWRVGDGL 443

Query: 61  SIRVYSDNWIPMDGSLSIRSP 82
           ++ +Y  NWIP DG+L + SP
Sbjct: 444 NVPIYGSNWIPRDGNLHVHSP 464

BLAST of MS023168 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 5.7e-18
Identity = 41/80 (51.25%), Postives = 58/80 (72.50%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           WR+L+ P+S+L+RVLK +YFK+  F+ A+   N S+IWRS+  GRDLL+ G  WR+GNG 
Sbjct: 664 WRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSILWGRDLLKKGLRWRIGNGD 723

Query: 61  SIRVYSDNWIPMDGSLSIRS 81
           S+ +Y DNW+P   +L I S
Sbjct: 724 SVFIYGDNWVPNQPTLKILS 743

BLAST of MS023168 vs. ExPASy TrEMBL
Match: A0A2I0A354 (Ribonuclease H protein OS=Apostasia shenzhenica OX=1088818 GN=AXF42_Ash017513 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 7.5e-18
Identity = 45/82 (54.88%), Postives = 60/82 (73.17%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           WR+++  +SL+ARVLK KYF N DFL A+A  N+S+IWRS+  GRDLL+ G  WR+GNG 
Sbjct: 295 WRIITHSNSLMARVLKAKYFHNCDFLMAQASPNSSYIWRSILWGRDLLKLGLRWRIGNGN 354

Query: 61  SIRVYSDNWIPMDGSL-SIRSP 82
           S+RV+ D W+P   S  +I SP
Sbjct: 355 SVRVFGDPWLPRPISFQAITSP 376

BLAST of MS023168 vs. ExPASy TrEMBL
Match: A0A803Q7Q3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 7.5e-18
Identity = 41/81 (50.62%), Postives = 53/81 (65.43%), Query Frame = 0

Query: 1    WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
            WRL+  P SLLARVLK  Y+ N  FL+A+   NAS IW+ +  GRD++  G WWRVGNG+
Sbjct: 946  WRLIHKPHSLLARVLKNSYYPNSSFLNAKCPQNASCIWKGIVWGRDIINEGSWWRVGNGR 1005

Query: 61   SIRVYSDNWIPMDGSLSIRSP 82
            +IRV+ D W+P      I  P
Sbjct: 1006 TIRVWQDKWLPRPNGTIIHRP 1026

BLAST of MS023168 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 9.3e-13
Identity = 30/70 (42.86%), Postives = 45/70 (64.29%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           WR+LS P SL+A+V K +YF   D L+A  GS  SF+W+S+   +++LR G    VGNG+
Sbjct: 74  WRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEILRQGARAVVGNGE 133

Query: 61  SIRVYSDNWI 71
            I ++   W+
Sbjct: 134 DIIIWRHKWL 143

BLAST of MS023168 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 60.5 bits (145), Expect = 7.4e-10
Identity = 24/70 (34.29%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 1   WRLLSDPSSLLARVLKFKYFKNGDFLSARAGSNASFIWRSLFQGRDLLRAGPWWRVGNGK 60
           +R++  P +LL+R+L+ +YF +   +    G+  S+ WRS+  GR+LL  G    +G+G 
Sbjct: 75  FRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGI 134

Query: 61  SIRVYSDNWI 71
             +V+ D WI
Sbjct: 135 HTKVWLDRWI 144

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159057.12.2e-2470.00uncharacterized protein LOC111025497 [Momordica charantia][more]
XP_022149385.12.8e-1955.56uncharacterized protein LOC111017816 [Momordica charantia][more]
XP_042944517.18.2e-1953.09uncharacterized protein LOC122278389 [Carya illinoinensis][more]
KAF5458995.13.1e-1855.56hypothetical protein F2P56_022987 [Juglans regia][more]
XP_024950112.15.3e-1856.34uncharacterized protein LOC112496847 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
P932951.0e-0834.29Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DYS71.1e-2470.00uncharacterized protein LOC111025497 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1D5K11.4e-1955.56uncharacterized protein LOC111017816 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1DAR45.7e-1851.25uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A2I0A3547.5e-1854.88Ribonuclease H protein OS=Apostasia shenzhenica OX=1088818 GN=AXF42_Ash017513 PE... [more]
A0A803Q7Q37.5e-1850.62Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G29090.19.3e-1342.86Ribonuclease H-like superfamily protein [more]
ATMG00310.17.4e-1034.29RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS023168.1MS023168.1mRNA