Homology
BLAST of MS023163 vs. NCBI nr
Match:
XP_022147590.1 (putative pectinesterase 63 [Momordica charantia])
HSP 1 Score: 781.6 bits (2017), Expect = 3.2e-222
Identity = 388/389 (99.74%), Postives = 389/389 (100.00%), Query Frame = 0
Query: 1 MYRPAVASSSPVAVAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERK 60
MYRPAVASSSPVAVAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERK
Sbjct: 1 MYRPAVASSSPVAVAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERK 60
Query: 61 ANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIY 120
ANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIY
Sbjct: 61 ANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIY 120
Query: 121 REKITIDASRPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSA 180
REKITIDAS+PFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSA
Sbjct: 121 REKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSA 180
Query: 181 AMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGN 240
AMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGN
Sbjct: 181 AMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGN 240
Query: 241 GKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTRVV 300
GKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTRVV
Sbjct: 241 GKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTRVV 300
Query: 301 FAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAF 360
FAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAF
Sbjct: 301 FAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAF 360
Query: 361 LSMTYIHGNKWLLPPPLLSFSRSHPNTFH 390
LSMTYIHGNKWLLPPPLLSFSRSHPNTFH
Sbjct: 361 LSMTYIHGNKWLLPPPLLSFSRSHPNTFH 389
BLAST of MS023163 vs. NCBI nr
Match:
XP_038880021.1 (putative pectinesterase 63 [Benincasa hispida])
HSP 1 Score: 657.9 bits (1696), Expect = 5.4e-185
Identity = 325/391 (83.12%), Postives = 352/391 (90.03%), Query Frame = 0
Query: 1 MYRPAVASSSPVAVAIALAVIQLIIIL--PAAVVARTKPIPAEPSKLDEWIENNMKEYHE 60
M RP VASSSP AVA++LA+ QLII++ A V ARTK IPA+ SKLD+WI +NMKEY E
Sbjct: 1 MSRPTVASSSPAAVAVSLALFQLIILIISSAVVEARTKMIPADASKLDQWIAHNMKEYEE 60
Query: 61 RKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGG 120
RKAN+TG+KALD+RLA AED VRLITVR DGRGNFTTIT AIESIP+GN RV+VWI GG
Sbjct: 61 RKANETGIKALDRRLAKAEDHVRLITVRKDGRGNFTTITKAIESIPSGNRRRVVVWIGGG 120
Query: 121 IYREKITIDASRPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVN 180
+YREKITI+AS+PFVTLYGQ G+ PVITFDGTASEFGTV+SATVAVESDYFVAVN+AFVN
Sbjct: 121 VYREKITIEASKPFVTLYGQKGRRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVN 180
Query: 181 SAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIF 240
SA MP++ +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCYI+GTVDFIF
Sbjct: 181 SAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIF 240
Query: 241 GNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTR 300
GNGKSLYLKT INSVAEG GVITAQARE+ATDDSGFTF YCNITGTGNTYLGRAWKERTR
Sbjct: 241 GNGKSLYLKTIINSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERTR 300
Query: 301 VVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEAR 360
VVFAYTYMG LINTEGWSDKMH +PRKSMYYGEYKCMGPGATP+GRVKYARILSDEEA+
Sbjct: 301 VVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAK 360
Query: 361 AFLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 390
AFLSMTYIHGNKWLLPPP LS S N FH
Sbjct: 361 AFLSMTYIHGNKWLLPPPNLSLFHSRSNVFH 391
BLAST of MS023163 vs. NCBI nr
Match:
XP_008450966.1 (PREDICTED: putative pectinesterase 63 [Cucumis melo])
HSP 1 Score: 644.4 bits (1661), Expect = 6.2e-181
Identity = 321/394 (81.47%), Postives = 353/394 (89.59%), Query Frame = 0
Query: 1 MYRPAVASSSPVAVAIALAVI-QLIII--LPAAVV--ARTKPIPAEPSKLDEWIENNMKE 60
M+RP VASS VA+ALAV+ QLII+ +P+ V+ ARTK IPA+ SKLDEWI +NM+E
Sbjct: 1 MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
Query: 61 YHERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWI 120
Y++RK N+TG+KALD+RLA AED VRLITVR DGRGNF+TIT AIESIP+GN RV+VWI
Sbjct: 61 YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
Query: 121 AGGIYREKITIDASRPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIA 180
GG+YREKITIDAS+PFVTLYGQ GK PVITFDGTASEFGTV+SATVAVESDYFVAVN+
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
Query: 181 FVNSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVD 240
FVNSA MP++ +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
Query: 241 FIFGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKE 300
FIFGNGKSLYLKT INSVAEG+GVITAQAREDATD+SGFTF YCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
Query: 301 RTRVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDE 360
RTRVVFAYTYMG LINTEGWSDKMH +PRKSMYYGEYKC GPGAT +GRVKYARILSD
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
Query: 361 EARAFLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 390
EA+AFLSMTYIHGNKWLLPPP LSFS S PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394
BLAST of MS023163 vs. NCBI nr
Match:
XP_004144054.1 (putative pectinesterase 63 [Cucumis sativus] >KGN66331.1 hypothetical protein Csa_007174 [Cucumis sativus])
HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-179
Identity = 318/394 (80.71%), Postives = 350/394 (88.83%), Query Frame = 0
Query: 1 MYRPAVASSSPVAVAIALAV-IQLI--IILPAAVV--ARTKPIPAEPSKLDEWIENNMKE 60
M+RP VASS P VA A+ V +QLI II+P+ VV A TK IPA+ SKLDEWI +NMKE
Sbjct: 1 MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
Query: 61 YHERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWI 120
Y++RK N+TG+KALD+RLA AED V+LITVR DGRGNF+TIT AI+SIP+GN RV+VWI
Sbjct: 61 YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
Query: 121 AGGIYREKITIDASRPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIA 180
GG+YREKITIDAS+PFVTLYGQ GK P+ITFDGTASEFGTV+SATVAVESDYFVAVN+
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
Query: 181 FVNSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVD 240
FVNSA MP++ +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCY++GTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
Query: 241 FIFGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKE 300
FIFGNGKSLYLKT INSVAEG GVITAQAREDATD+SGFTF YCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
Query: 301 RTRVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDE 360
RTRVVFAYTYMG LINTEGWSDKMH +PRKSMYYGEYKC GPGATP+GRVKYARILSD
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
Query: 361 EARAFLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 390
EA+AFLSMTYIHGNKWLLPPP LS S S PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394
BLAST of MS023163 vs. NCBI nr
Match:
XP_022987214.1 (putative pectinesterase 63 [Cucurbita maxima])
HSP 1 Score: 626.3 bits (1614), Expect = 1.7e-175
Identity = 308/382 (80.63%), Postives = 341/382 (89.27%), Query Frame = 0
Query: 1 MYRPAVASSSPVAVAIALAVIQLI-IILPAAVV--ARTKPIPAEPSKLDEWIENNMKEYH 60
M RPAVASS P VA+ LAV+QLI +++PAA+V ARTKPIPAEPS+LDEWI +NM+EY
Sbjct: 1 MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60
Query: 61 ERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAG 120
+RKA +TG KALD+RLA AED RLITV+ DG+GNF+TIT AIESIP+GN+ RV+VWI G
Sbjct: 61 QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120
Query: 121 GIYREKITIDASRPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFV 180
G YREKITID+S+PFVTLYGQ GKMPVI++DGTASEFGTV+SATVAVESDYFVAVN+AFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180
Query: 181 NSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
NSA MPD +GGQAVAMRISGDKAAFHHC FIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
Query: 241 FGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERT 300
FGNGKSLYLKT INSVAEG GVITAQARE++TD+SGFTF YCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300
Query: 301 RVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEA 360
RVVFAYTYMG LINT+GWSDKM EPRKSMYYGEYKC GPGA GR Y RIL+D+EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360
Query: 361 RAFLSMTYIHGNKWLLPPPLLS 380
+AFLSMTYIHGNKW+LPPP LS
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLS 382
BLAST of MS023163 vs. ExPASy Swiss-Prot
Match:
Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)
HSP 1 Score: 357.1 bits (915), Expect = 2.6e-97
Identity = 176/364 (48.35%), Postives = 248/364 (68.13%), Query Frame = 0
Query: 16 IALAVIQLIIIL--PAAVVARTKPIPAEPSKLDEWIENNMKEYHERKANDTGVKALDKRL 75
I+++V+ +++ P + T PIP +++ +W + N+K Y +RK LD L
Sbjct: 4 ISMSVVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKG------TLDPAL 63
Query: 76 AMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIYREKITIDASRPFV 135
AE A ++ITV G NF T+ AI+SIP GN RVI+ +A G+Y EK+TID +RPF+
Sbjct: 64 EAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFI 123
Query: 136 TLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSAAMPDVRVSGGQAV 195
TL GQ G V+T+ GTA+++GTV+SAT+ V ++YF A ++ N+A MP S GQA+
Sbjct: 124 TLLGQPGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPG-SQGQAL 183
Query: 196 AMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTRINSV 255
AMRI+ DKAAF+ C F GFQDTLCDD+G HFFKDCYIEGT DFIFG G SLYL T++++V
Sbjct: 184 AMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAV 243
Query: 256 AEGMGVITAQAREDATDDSGFTFVYCNITGTG-NTYLGRAWKERTRVVFAYTYMGNLINT 315
+G+ VITAQ R+ AT+ +G+TFV+C +TGTG YLGR+W +VV+A+T M +++N
Sbjct: 244 GDGLRVITAQGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNP 303
Query: 316 EGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAFLSMTYIHGNKWL 375
GW + ++ G K+++YGEYKC GPG+ RV Y + + E FL++ YI G+ WL
Sbjct: 304 SGWRENLNRGYD-KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWL 359
Query: 376 LPPP 377
LPPP
Sbjct: 364 LPPP 359
BLAST of MS023163 vs. ExPASy Swiss-Prot
Match:
Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)
HSP 1 Score: 355.9 bits (912), Expect = 5.7e-97
Identity = 175/364 (48.08%), Postives = 240/364 (65.93%), Query Frame = 0
Query: 14 VAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERKANDTGVKALDKRL 73
V++ + ++ ++I P PIP ++++W N+K+
Sbjct: 6 VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTNVKQ------------------ 65
Query: 74 AMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIYREKITIDASRPFV 133
+GRG+F TIT AI S+ AGNT RVI+ I G+Y+EK+TID S+PF+
Sbjct: 66 --------------NGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFI 125
Query: 134 TLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSAAMPDVRVSGGQAV 193
TLYG MPV+TFDGTA+++GTV SAT+ V SDYF+AVNI NSA MPD + G QA+
Sbjct: 126 TLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQAL 185
Query: 194 AMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTRINSV 253
+MRISG+KAAF++C F G+QDT+CDD G HFFKDCYIEGT DFIFG+G+SLYL T++N V
Sbjct: 186 SMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVV 245
Query: 254 AEGMGVITAQAREDATDDSGFTFVYCNITGTG-NTYLGRAWKERTRVVFAYTYMGNLINT 313
+G+ VITA A + A + SG++FV+C +TGTG YLGR+W +VV+AYT M +++N
Sbjct: 246 GDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNP 305
Query: 314 EGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAFLSMTYIHGNKWL 373
GW + G K+++YGEYKC G G+ RVKY + + D EA+ F+S+ YI G+ WL
Sbjct: 306 SGWQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWL 336
Query: 374 LPPP 377
LPPP
Sbjct: 366 LPPP 336
BLAST of MS023163 vs. ExPASy Swiss-Prot
Match:
Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)
HSP 1 Score: 355.5 bits (911), Expect = 7.5e-97
Identity = 180/366 (49.18%), Postives = 248/366 (67.76%), Query Frame = 0
Query: 14 VAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERKANDTGVKALDKRL 73
V+I L ++ ++ + P PIP ++ +W N+ +R K LD L
Sbjct: 6 VSILLGLL-MVFVTPMVFADDVTPIPEGKPQVAQWFNANVGPLAQR-------KGLDPAL 65
Query: 74 AMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIYREKITIDASRPFV 133
AE A R+I V P G G F T+T AI+S+PAGNT RVI+ +A G Y+EK+TID ++PF+
Sbjct: 66 VAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFI 125
Query: 134 TLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSAAMPDVRVSGGQAV 193
TL GQ MPVIT+DGTA+++GTV SA++ + SDYF+AVNI N+A PD + G QA+
Sbjct: 126 TLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQAL 185
Query: 194 AMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTRINSV 253
+MRISG+ AAF++C F GFQDT+CDD G HFFKDCY+EGT DFIFG+G S+YL T+++ V
Sbjct: 186 SMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVV 245
Query: 254 AEGMGVITAQAREDATDDSGFTFVYCNITGT-GNTYLGRAWKERTRVVFAYTYMGNLINT 313
+G+ VI A A + A + SG++FV+C +TGT G YLGRAW +VV+AYT M +++N
Sbjct: 246 GDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNP 305
Query: 314 EGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAFLSMTYIHGNKWL 373
GW + K+++YGEYKC GPG+ A RV + + + D+EA FLS+ YI G+KWL
Sbjct: 306 TGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWL 361
Query: 374 LPPPLL 379
LPPP L
Sbjct: 366 LPPPAL 361
BLAST of MS023163 vs. ExPASy Swiss-Prot
Match:
Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)
HSP 1 Score: 354.8 bits (909), Expect = 1.3e-96
Identity = 181/366 (49.45%), Postives = 248/366 (67.76%), Query Frame = 0
Query: 14 VAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERKANDTGVKALDKRL 73
V+I L ++ +I + P PIP ++ +W ++ +R K LD L
Sbjct: 6 VSILLGML-VIFVSPMVFADDLTPIPEGKPQVVQWFNTHVGPLAQR-------KGLDPAL 65
Query: 74 AMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIYREKITIDASRPFV 133
AE A R+I V P G G F T+T AI+S+PAGNT RVI+ +A G YREK+TID ++PF+
Sbjct: 66 VAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFI 125
Query: 134 TLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSAAMPDVRVSGGQAV 193
TL GQ MPVIT+DGTA+++GTV SA++ + SDYF+AVNI N+A PD + G QA+
Sbjct: 126 TLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQAL 185
Query: 194 AMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTRINSV 253
+MRISG+ AAF++C F GFQDT+CDD G HFFKDCY+EGT DFIFG+G S+YL T+++ V
Sbjct: 186 SMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVV 245
Query: 254 AEGMGVITAQAREDATDDSGFTFVYCNITGT-GNTYLGRAWKERTRVVFAYTYMGNLINT 313
+G+ VI A A + A + SG++FV+C +TGT G YLGRAW +VV+AYT M +++N
Sbjct: 246 GDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNP 305
Query: 314 EGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAFLSMTYIHGNKWL 373
GW + K+++YGEYKC GPG+ A RV + + + D+EA FLS+ YI G+KWL
Sbjct: 306 TGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWL 361
Query: 374 LPPPLL 379
LPPP L
Sbjct: 366 LPPPAL 361
BLAST of MS023163 vs. ExPASy Swiss-Prot
Match:
Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)
HSP 1 Score: 339.3 bits (869), Expect = 5.6e-92
Identity = 170/364 (46.70%), Postives = 246/364 (67.58%), Query Frame = 0
Query: 16 IALAVIQLIIILPAAVVAR--TKPIPAEPSKLDEWIENNMKEYHERKANDTGVKALDKRL 75
I+LA++ L++ + VV PIPA+ +++ +W N+K + +R+ LD L
Sbjct: 4 ISLALVALLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRG------TLDPEL 63
Query: 76 AMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIYREKITIDASRPFV 135
AE + R+I V +G G+F TI AAI+SIP N RVI+ +A GIY EK+T+D RP+V
Sbjct: 64 EAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYV 123
Query: 136 TLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSAAMPDVRVSGGQAV 195
TL G+ G +T+ GTA+++GTV+SAT+ V + F+A N+ +N++ MP GQA+
Sbjct: 124 TLLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPGTQ-GQAL 183
Query: 196 AMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTRINSV 255
AMRI+GDKAAF++C F GFQDTLCDDRG HFFK+CYIEGT DFIFG G SLYL T++++V
Sbjct: 184 AMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAV 243
Query: 256 AEGMGVITAQAREDATDDSGFTFVYCNITGTG-NTYLGRAWKERTRVVFAYTYMGNLINT 315
+G+ VI A R+ T+ +G++FV+C +TG G YLGRAW +VV++YT M +++N
Sbjct: 244 GDGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNP 303
Query: 316 EGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAFLSMTYIHGNKWL 375
GW + K+++YGEY C GPG+ A RV + + + ++EA FL++ YI G+KWL
Sbjct: 304 SGWQENRVRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWL 359
Query: 376 LPPP 377
LPPP
Sbjct: 364 LPPP 359
BLAST of MS023163 vs. ExPASy TrEMBL
Match:
A0A6J1D0K0 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1)
HSP 1 Score: 781.6 bits (2017), Expect = 1.6e-222
Identity = 388/389 (99.74%), Postives = 389/389 (100.00%), Query Frame = 0
Query: 1 MYRPAVASSSPVAVAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERK 60
MYRPAVASSSPVAVAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERK
Sbjct: 1 MYRPAVASSSPVAVAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERK 60
Query: 61 ANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIY 120
ANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIY
Sbjct: 61 ANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIY 120
Query: 121 REKITIDASRPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSA 180
REKITIDAS+PFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSA
Sbjct: 121 REKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSA 180
Query: 181 AMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGN 240
AMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGN
Sbjct: 181 AMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGN 240
Query: 241 GKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTRVV 300
GKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTRVV
Sbjct: 241 GKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTRVV 300
Query: 301 FAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAF 360
FAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAF
Sbjct: 301 FAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAF 360
Query: 361 LSMTYIHGNKWLLPPPLLSFSRSHPNTFH 390
LSMTYIHGNKWLLPPPLLSFSRSHPNTFH
Sbjct: 361 LSMTYIHGNKWLLPPPLLSFSRSHPNTFH 389
BLAST of MS023163 vs. ExPASy TrEMBL
Match:
A0A1S3BRI5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1)
HSP 1 Score: 644.4 bits (1661), Expect = 3.0e-181
Identity = 321/394 (81.47%), Postives = 353/394 (89.59%), Query Frame = 0
Query: 1 MYRPAVASSSPVAVAIALAVI-QLIII--LPAAVV--ARTKPIPAEPSKLDEWIENNMKE 60
M+RP VASS VA+ALAV+ QLII+ +P+ V+ ARTK IPA+ SKLDEWI +NM+E
Sbjct: 1 MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
Query: 61 YHERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWI 120
Y++RK N+TG+KALD+RLA AED VRLITVR DGRGNF+TIT AIESIP+GN RV+VWI
Sbjct: 61 YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
Query: 121 AGGIYREKITIDASRPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIA 180
GG+YREKITIDAS+PFVTLYGQ GK PVITFDGTASEFGTV+SATVAVESDYFVAVN+
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
Query: 181 FVNSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVD 240
FVNSA MP++ +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
Query: 241 FIFGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKE 300
FIFGNGKSLYLKT INSVAEG+GVITAQAREDATD+SGFTF YCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
Query: 301 RTRVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDE 360
RTRVVFAYTYMG LINTEGWSDKMH +PRKSMYYGEYKC GPGAT +GRVKYARILSD
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
Query: 361 EARAFLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 390
EA+AFLSMTYIHGNKWLLPPP LSFS S PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394
BLAST of MS023163 vs. ExPASy TrEMBL
Match:
A0A0A0M299 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1)
HSP 1 Score: 640.2 bits (1650), Expect = 5.6e-180
Identity = 318/394 (80.71%), Postives = 350/394 (88.83%), Query Frame = 0
Query: 1 MYRPAVASSSPVAVAIALAV-IQLI--IILPAAVV--ARTKPIPAEPSKLDEWIENNMKE 60
M+RP VASS P VA A+ V +QLI II+P+ VV A TK IPA+ SKLDEWI +NMKE
Sbjct: 1 MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
Query: 61 YHERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWI 120
Y++RK N+TG+KALD+RLA AED V+LITVR DGRGNF+TIT AI+SIP+GN RV+VWI
Sbjct: 61 YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
Query: 121 AGGIYREKITIDASRPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIA 180
GG+YREKITIDAS+PFVTLYGQ GK P+ITFDGTASEFGTV+SATVAVESDYFVAVN+
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
Query: 181 FVNSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVD 240
FVNSA MP++ +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCY++GTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
Query: 241 FIFGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKE 300
FIFGNGKSLYLKT INSVAEG GVITAQAREDATD+SGFTF YCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
Query: 301 RTRVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDE 360
RTRVVFAYTYMG LINTEGWSDKMH +PRKSMYYGEYKC GPGATP+GRVKYARILSD
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
Query: 361 EARAFLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 390
EA+AFLSMTYIHGNKWLLPPP LS S S PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394
BLAST of MS023163 vs. ExPASy TrEMBL
Match:
A0A6J1JI92 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1)
HSP 1 Score: 626.3 bits (1614), Expect = 8.4e-176
Identity = 308/382 (80.63%), Postives = 341/382 (89.27%), Query Frame = 0
Query: 1 MYRPAVASSSPVAVAIALAVIQLI-IILPAAVV--ARTKPIPAEPSKLDEWIENNMKEYH 60
M RPAVASS P VA+ LAV+QLI +++PAA+V ARTKPIPAEPS+LDEWI +NM+EY
Sbjct: 1 MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60
Query: 61 ERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAG 120
+RKA +TG KALD+RLA AED RLITV+ DG+GNF+TIT AIESIP+GN+ RV+VWI G
Sbjct: 61 QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120
Query: 121 GIYREKITIDASRPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFV 180
G YREKITID+S+PFVTLYGQ GKMPVI++DGTASEFGTV+SATVAVESDYFVAVN+AFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180
Query: 181 NSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
NSA MPD +GGQAVAMRISGDKAAFHHC FIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
Query: 241 FGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERT 300
FGNGKSLYLKT INSVAEG GVITAQARE++TD+SGFTF YCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300
Query: 301 RVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEA 360
RVVFAYTYMG LINT+GWSDKM EPRKSMYYGEYKC GPGA GR Y RIL+D+EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360
Query: 361 RAFLSMTYIHGNKWLLPPPLLS 380
+AFLSMTYIHGNKW+LPPP LS
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLS 382
BLAST of MS023163 vs. ExPASy TrEMBL
Match:
A0A6J1HB45 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1)
HSP 1 Score: 623.2 bits (1606), Expect = 7.1e-175
Identity = 308/382 (80.63%), Postives = 337/382 (88.22%), Query Frame = 0
Query: 1 MYRPAVASSSPVAVAIALAVIQLI-IILPAAVV--ARTKPIPAEPSKLDEWIENNMKEYH 60
M RPAVASS P VA+ LAV+QLI I++PAA+V ARTK IPAEPS+LDEWI +NM+EY
Sbjct: 1 MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60
Query: 61 ERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAG 120
+RKAN+TG K LD RLA AED RLITV+ DG+GNF+TIT AIESIP+GN RV+VWI G
Sbjct: 61 QRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120
Query: 121 GIYREKITIDASRPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFV 180
G YREKITID+S+PFVTLYGQ GKMPVI++DGTASEFGT +SATVAVESDYFVAVN+AFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180
Query: 181 NSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
NSA MPD +GGQAVAMRISGDKAAFHHC FIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
Query: 241 FGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERT 300
FGNGKSLYLKT INSVAEG GVITAQARE++TDDSGFTF YCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300
Query: 301 RVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEA 360
RVVFAYTYMG LINT+GWSDKM EPRKSMYYGEYKC GPGA GR Y RIL+D+EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360
Query: 361 RAFLSMTYIHGNKWLLPPPLLS 380
+AFLSMTYIHGNKW+LPPP LS
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLS 382
BLAST of MS023163 vs. TAIR 10
Match:
AT5G07430.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 357.1 bits (915), Expect = 1.8e-98
Identity = 176/364 (48.35%), Postives = 248/364 (68.13%), Query Frame = 0
Query: 16 IALAVIQLIIIL--PAAVVARTKPIPAEPSKLDEWIENNMKEYHERKANDTGVKALDKRL 75
I+++V+ +++ P + T PIP +++ +W + N+K Y +RK LD L
Sbjct: 4 ISMSVVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKG------TLDPAL 63
Query: 76 AMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIYREKITIDASRPFV 135
AE A ++ITV G NF T+ AI+SIP GN RVI+ +A G+Y EK+TID +RPF+
Sbjct: 64 EAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFI 123
Query: 136 TLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSAAMPDVRVSGGQAV 195
TL GQ G V+T+ GTA+++GTV+SAT+ V ++YF A ++ N+A MP S GQA+
Sbjct: 124 TLLGQPGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPG-SQGQAL 183
Query: 196 AMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTRINSV 255
AMRI+ DKAAF+ C F GFQDTLCDD+G HFFKDCYIEGT DFIFG G SLYL T++++V
Sbjct: 184 AMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAV 243
Query: 256 AEGMGVITAQAREDATDDSGFTFVYCNITGTG-NTYLGRAWKERTRVVFAYTYMGNLINT 315
+G+ VITAQ R+ AT+ +G+TFV+C +TGTG YLGR+W +VV+A+T M +++N
Sbjct: 244 GDGLRVITAQGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNP 303
Query: 316 EGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAFLSMTYIHGNKWL 375
GW + ++ G K+++YGEYKC GPG+ RV Y + + E FL++ YI G+ WL
Sbjct: 304 SGWRENLNRGYD-KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWL 359
Query: 376 LPPP 377
LPPP
Sbjct: 364 LPPP 359
BLAST of MS023163 vs. TAIR 10
Match:
AT5G61680.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 355.9 bits (912), Expect = 4.1e-98
Identity = 175/364 (48.08%), Postives = 240/364 (65.93%), Query Frame = 0
Query: 14 VAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERKANDTGVKALDKRL 73
V++ + ++ ++I P PIP ++++W N+K+
Sbjct: 6 VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTNVKQ------------------ 65
Query: 74 AMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIYREKITIDASRPFV 133
+GRG+F TIT AI S+ AGNT RVI+ I G+Y+EK+TID S+PF+
Sbjct: 66 --------------NGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFI 125
Query: 134 TLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSAAMPDVRVSGGQAV 193
TLYG MPV+TFDGTA+++GTV SAT+ V SDYF+AVNI NSA MPD + G QA+
Sbjct: 126 TLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQAL 185
Query: 194 AMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTRINSV 253
+MRISG+KAAF++C F G+QDT+CDD G HFFKDCYIEGT DFIFG+G+SLYL T++N V
Sbjct: 186 SMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVV 245
Query: 254 AEGMGVITAQAREDATDDSGFTFVYCNITGTG-NTYLGRAWKERTRVVFAYTYMGNLINT 313
+G+ VITA A + A + SG++FV+C +TGTG YLGR+W +VV+AYT M +++N
Sbjct: 246 GDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNP 305
Query: 314 EGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAFLSMTYIHGNKWL 373
GW + G K+++YGEYKC G G+ RVKY + + D EA+ F+S+ YI G+ WL
Sbjct: 306 SGWQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWL 336
Query: 374 LPPP 377
LPPP
Sbjct: 366 LPPP 336
BLAST of MS023163 vs. TAIR 10
Match:
AT1G69940.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 355.5 bits (911), Expect = 5.3e-98
Identity = 180/366 (49.18%), Postives = 248/366 (67.76%), Query Frame = 0
Query: 14 VAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERKANDTGVKALDKRL 73
V+I L ++ ++ + P PIP ++ +W N+ +R K LD L
Sbjct: 6 VSILLGLL-MVFVTPMVFADDVTPIPEGKPQVAQWFNANVGPLAQR-------KGLDPAL 65
Query: 74 AMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIYREKITIDASRPFV 133
AE A R+I V P G G F T+T AI+S+PAGNT RVI+ +A G Y+EK+TID ++PF+
Sbjct: 66 VAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFI 125
Query: 134 TLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSAAMPDVRVSGGQAV 193
TL GQ MPVIT+DGTA+++GTV SA++ + SDYF+AVNI N+A PD + G QA+
Sbjct: 126 TLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQAL 185
Query: 194 AMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTRINSV 253
+MRISG+ AAF++C F GFQDT+CDD G HFFKDCY+EGT DFIFG+G S+YL T+++ V
Sbjct: 186 SMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVV 245
Query: 254 AEGMGVITAQAREDATDDSGFTFVYCNITGT-GNTYLGRAWKERTRVVFAYTYMGNLINT 313
+G+ VI A A + A + SG++FV+C +TGT G YLGRAW +VV+AYT M +++N
Sbjct: 246 GDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNP 305
Query: 314 EGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAFLSMTYIHGNKWL 373
GW + K+++YGEYKC GPG+ A RV + + + D+EA FLS+ YI G+KWL
Sbjct: 306 TGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWL 361
Query: 374 LPPPLL 379
LPPP L
Sbjct: 366 LPPPAL 361
BLAST of MS023163 vs. TAIR 10
Match:
AT5G07410.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 354.8 bits (909), Expect = 9.1e-98
Identity = 181/366 (49.45%), Postives = 248/366 (67.76%), Query Frame = 0
Query: 14 VAIALAVIQLIIILPAAVVARTKPIPAEPSKLDEWIENNMKEYHERKANDTGVKALDKRL 73
V+I L ++ +I + P PIP ++ +W ++ +R K LD L
Sbjct: 6 VSILLGML-VIFVSPMVFADDLTPIPEGKPQVVQWFNTHVGPLAQR-------KGLDPAL 65
Query: 74 AMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIYREKITIDASRPFV 133
AE A R+I V P G G F T+T AI+S+PAGNT RVI+ +A G YREK+TID ++PF+
Sbjct: 66 VAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFI 125
Query: 134 TLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSAAMPDVRVSGGQAV 193
TL GQ MPVIT+DGTA+++GTV SA++ + SDYF+AVNI N+A PD + G QA+
Sbjct: 126 TLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQAL 185
Query: 194 AMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTRINSV 253
+MRISG+ AAF++C F GFQDT+CDD G HFFKDCY+EGT DFIFG+G S+YL T+++ V
Sbjct: 186 SMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVV 245
Query: 254 AEGMGVITAQAREDATDDSGFTFVYCNITGT-GNTYLGRAWKERTRVVFAYTYMGNLINT 313
+G+ VI A A + A + SG++FV+C +TGT G YLGRAW +VV+AYT M +++N
Sbjct: 246 GDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNP 305
Query: 314 EGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAFLSMTYIHGNKWL 373
GW + K+++YGEYKC GPG+ A RV + + + D+EA FLS+ YI G+KWL
Sbjct: 306 TGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWL 361
Query: 374 LPPPLL 379
LPPP L
Sbjct: 366 LPPPAL 361
BLAST of MS023163 vs. TAIR 10
Match:
AT5G07420.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 339.3 bits (869), Expect = 4.0e-93
Identity = 170/364 (46.70%), Postives = 246/364 (67.58%), Query Frame = 0
Query: 16 IALAVIQLIIILPAAVVAR--TKPIPAEPSKLDEWIENNMKEYHERKANDTGVKALDKRL 75
I+LA++ L++ + VV PIPA+ +++ +W N+K + +R+ LD L
Sbjct: 4 ISLALVALLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRG------TLDPEL 63
Query: 76 AMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGIYREKITIDASRPFV 135
AE + R+I V +G G+F TI AAI+SIP N RVI+ +A GIY EK+T+D RP+V
Sbjct: 64 EAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYV 123
Query: 136 TLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNSAAMPDVRVSGGQAV 195
TL G+ G +T+ GTA+++GTV+SAT+ V + F+A N+ +N++ MP GQA+
Sbjct: 124 TLLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPGTQ-GQAL 183
Query: 196 AMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTRINSV 255
AMRI+GDKAAF++C F GFQDTLCDDRG HFFK+CYIEGT DFIFG G SLYL T++++V
Sbjct: 184 AMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAV 243
Query: 256 AEGMGVITAQAREDATDDSGFTFVYCNITGTG-NTYLGRAWKERTRVVFAYTYMGNLINT 315
+G+ VI A R+ T+ +G++FV+C +TG G YLGRAW +VV++YT M +++N
Sbjct: 244 GDGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNP 303
Query: 316 EGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARAFLSMTYIHGNKWL 375
GW + K+++YGEY C GPG+ A RV + + + ++EA FL++ YI G+KWL
Sbjct: 304 SGWQENRVRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWL 359
Query: 376 LPPP 377
LPPP
Sbjct: 364 LPPP 359
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9LY17 | 2.6e-97 | 48.35 | Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1 | [more] |
Q9FKF3 | 5.7e-97 | 48.08 | Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2 | [more] |
Q84WM7 | 7.5e-97 | 49.18 | Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1 | [more] |
Q9LY19 | 1.3e-96 | 49.45 | Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2 | [more] |
Q9LY18 | 5.6e-92 | 46.70 | Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1D0K0 | 1.6e-222 | 99.74 | Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1 | [more] |
A0A1S3BRI5 | 3.0e-181 | 81.47 | Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1 | [more] |
A0A0A0M299 | 5.6e-180 | 80.71 | Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1 | [more] |
A0A6J1JI92 | 8.4e-176 | 80.63 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1 | [more] |
A0A6J1HB45 | 7.1e-175 | 80.63 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1 | [more] |