MS023133 (gene) Bitter gourd (TR) v1

BLAST of MS023133 vs. NCBI nr
Match: XP_022147670.1 (tubulin--tyrosine ligase-like protein 12 [Momordica charantia])

HSP 1 Score: 1780.4 bits (4610), Expect = 0.0e+00
Identity = 862/867 (99.42%), Postives = 863/867 (99.54%), Query Frame = 0

Query: 1   MAAAGRIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 60
           MAAAGRIGTYEDFVEVHGLLLAAS LPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR
Sbjct: 78  MAAAGRIGTYEDFVEVHGLLLAASGLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 137

Query: 61  RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 120
           RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG
Sbjct: 138 RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 197

Query: 121 VHSNDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLLA 180
           VHSNDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLLA
Sbjct: 198 VHSNDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLLA 257

Query: 181 LSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEICNSRF 240
           LSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEI NSRF
Sbjct: 258 LSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEIYNSRF 317

Query: 241 THNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPSL 300
           THNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPSL
Sbjct: 318 THNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPSL 377

Query: 301 SYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGID 360
           SYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGID
Sbjct: 378 SYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGID 437

Query: 361 VAKILHSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWYV 420
           VAKILHSGKHVIDSML PRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWYV
Sbjct: 438 VAKILHSGKHVIDSMLRPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWYV 497

Query: 421 MDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGE 480
           MDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGE
Sbjct: 498 MDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGE 557

Query: 481 EKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVY 540
           EKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVY
Sbjct: 558 EKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVY 617

Query: 541 TDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVM 600
           TDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVM
Sbjct: 618 TDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVM 677

Query: 601 KHHLAETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT 660
           KHHLAETIQKAHGCAEWLQPTYNLE HLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT
Sbjct: 678 KHHLAETIQKAHGCAEWLQPTYNLEIHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT 737

Query: 661 TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR 720
           TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR
Sbjct: 738 TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR 797

Query: 721 LANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSMI 780
           LANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSMI
Sbjct: 798 LANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSMI 857

Query: 781 RSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEHVFED 840
           RSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVE+VFED
Sbjct: 858 RSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVENVFED 917

Query: 841 GVIKGREFYNHVFGCLFLNETTHVTPL 868
           GVIKGREFYNHVFGCLFLNETTHVTPL
Sbjct: 918 GVIKGREFYNHVFGCLFLNETTHVTPL 944

BLAST of MS023133 vs. NCBI nr
Match: XP_022933158.1 (tubulin--tyrosine ligase-like protein 12 [Cucurbita moschata])

HSP 1 Score: 1597.4 bits (4135), Expect = 0.0e+00
Identity = 772/868 (88.94%), Postives = 815/868 (93.89%), Query Frame = 0

Query: 1   MAAAGRIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 60
           MAA  RI  YEDFVEVHGLLLAAS LPQSLHRQLFQKLTSETFDGGAHFQVE FEDGRCR
Sbjct: 1   MAAGNRIEAYEDFVEVHGLLLAASGLPQSLHRQLFQKLTSETFDGGAHFQVEPFEDGRCR 60

Query: 61  RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 120
           RLVLSSD M+KESHVFLVDHAWTFRLSDAYNQL EV GLAERMASLMCVD+DMNLDE+  
Sbjct: 61  RLVLSSDFMSKESHVFLVDHAWTFRLSDAYNQLLEVPGLAERMASLMCVDVDMNLDED-- 120

Query: 121 VHSNDNEV-KQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLL 180
             + + EV +QSV+EL+ESEI G K+ GNG+VRWLELEDLQI+DDTLLSLDL TKFPDLL
Sbjct: 121 --AKEEEVDQQSVWELLESEIHGAKEKGNGSVRWLELEDLQIDDDTLLSLDLPTKFPDLL 180

Query: 181 ALSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEICNSR 240
           ALSLTGNKL NVDVVA+EV+KFKHLRALWLNNNP  ENC E LQQ+VL+G PNLEI NSR
Sbjct: 181 ALSLTGNKLSNVDVVAQEVAKFKHLRALWLNNNPAVENCDEILQQKVLDGSPNLEIYNSR 240

Query: 241 FTHNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPS 300
           FTHNFSEWALGFCGDIYEKDNPGSIYP DHSLQ + +LDLS+R IHNL NKAFSPVELPS
Sbjct: 241 FTHNFSEWALGFCGDIYEKDNPGSIYPADHSLQDVTSLDLSHRGIHNLNNKAFSPVELPS 300

Query: 301 LSYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGI 360
           LSYLNLRGNPL +NSV DLL ILKEFPCL+SLEVDIPGPLGEKA+DIIESLPNL+TLN I
Sbjct: 301 LSYLNLRGNPLEQNSVGDLLEILKEFPCLSSLEVDIPGPLGEKAVDIIESLPNLHTLNSI 360

Query: 361 DVAKILHSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWY 420
           DV KIL+SGKHVIDSMLLPR PEWTPEEPLPDRVINAMW YLMTYRLADEEKIDETSVWY
Sbjct: 361 DVKKILNSGKHVIDSMLLPRLPEWTPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWY 420

Query: 421 VMDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIG 480
           VMDELGSALRHSD+PNF+VAPFLFMP+GKL SAISFTILWPI +VQKGDECTRDYLFGIG
Sbjct: 421 VMDELGSALRHSDEPNFRVAPFLFMPDGKLASAISFTILWPILDVQKGDECTRDYLFGIG 480

Query: 481 EEKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHV 540
           E+KQRSARLTAWFHTPQNYF+HEYEK+IRNLQSK LASPI QT  NTEG  Q KGGTL V
Sbjct: 481 EDKQRSARLTAWFHTPQNYFVHEYEKYIRNLQSKALASPIPQTTSNTEGRHQIKGGTLRV 540

Query: 541 YTDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLV 600
           YTDNPQVEE+L+RPEF+ITSDPKEADIIWTSMQIDE+TKKATGITD+QY+NQFPFEACLV
Sbjct: 541 YTDNPQVEEYLNRPEFIITSDPKEADIIWTSMQIDEETKKATGITDQQYVNQFPFEACLV 600

Query: 601 MKHHLAETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTID 660
           MKHHLAET++KAHGC EWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTID
Sbjct: 601 MKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTID 660

Query: 661 TTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWV 720
           TTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWV
Sbjct: 661 TTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWV 720

Query: 721 RLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSM 780
           RLANNPYSLEKQSLFEYETHFTVMNYRG+LNHKNIA+FVKEFEQEHNVKWLDIHSRVRSM
Sbjct: 721 RLANNPYSLEKQSLFEYETHFTVMNYRGKLNHKNIADFVKEFEQEHNVKWLDIHSRVRSM 780

Query: 781 IRSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEHVFE 840
           IRSVFESAAVVHPEMHSPFSRAMYGLDVMLD+SFQPKLLEVTYCPDCTRACKYDVE+VF 
Sbjct: 781 IRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVENVFG 840

Query: 841 DGVIKGREFYNHVFGCLFLNETTHVTPL 868
           DG+IKGREFYN VFGCLFLNETTH TPL
Sbjct: 841 DGIIKGREFYNDVFGCLFLNETTHATPL 864

BLAST of MS023133 vs. NCBI nr
Match: XP_022966708.1 (tubulin--tyrosine ligase-like protein 12 [Cucurbita maxima])

HSP 1 Score: 1594.7 bits (4128), Expect = 0.0e+00
Identity = 770/867 (88.81%), Postives = 810/867 (93.43%), Query Frame = 0

Query: 1   MAAAGRIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 60
           MAA  RI  YEDFVEVHGLLLAAS LPQSLHRQLFQKLTSETFDGGAHFQVE FEDGRCR
Sbjct: 1   MAAGNRIEAYEDFVEVHGLLLAASGLPQSLHRQLFQKLTSETFDGGAHFQVEPFEDGRCR 60

Query: 61  RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 120
           RLVLSSD MAKESHVFLVDHAWTFRLSDAYNQL EV GLAERMASLMCVD+DMNLDE+  
Sbjct: 61  RLVLSSDFMAKESHVFLVDHAWTFRLSDAYNQLLEVPGLAERMASLMCVDVDMNLDEDVK 120

Query: 121 VHSNDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLLA 180
               D   +QSV+EL+ESEI G K+ GNG+VRWLELEDLQI+DDTLLSLDL TKFPDLLA
Sbjct: 121 EEEVD---QQSVWELLESEIHGAKEEGNGSVRWLELEDLQIDDDTLLSLDLPTKFPDLLA 180

Query: 181 LSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEICNSRF 240
           LSLTGNKL NVDVVA+EV+KFKHLRALWLNNNP  ENC E LQQ+VL+G PNLEI NSRF
Sbjct: 181 LSLTGNKLSNVDVVAQEVAKFKHLRALWLNNNPAVENCDEILQQKVLDGSPNLEIYNSRF 240

Query: 241 THNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPSL 300
           THNFSEWALGFCGDIYEKDNPGSIYP DHSLQ + +LDLS+RCIHNL NKAFSPVELPSL
Sbjct: 241 THNFSEWALGFCGDIYEKDNPGSIYPADHSLQDVTSLDLSHRCIHNLNNKAFSPVELPSL 300

Query: 301 SYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGID 360
           SYLNLRGNPL +NSV DLL ILK FPCL+SLEVDIPGPLGEKA+DIIESLPNL+TLN ID
Sbjct: 301 SYLNLRGNPLEQNSVGDLLEILKGFPCLSSLEVDIPGPLGEKAVDIIESLPNLHTLNSID 360

Query: 361 VAKILHSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWYV 420
           V KIL+SGKHVIDSMLLPR PEWTPEEPL DRVINAMW YLMTYRLADEEKIDETSVWYV
Sbjct: 361 VKKILNSGKHVIDSMLLPRLPEWTPEEPLRDRVINAMWQYLMTYRLADEEKIDETSVWYV 420

Query: 421 MDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGE 480
           MDELGSALRHSD+PNF+VAPFLFMP+GKL SAISFTILWPI +VQKGDECTRDYLFGIGE
Sbjct: 421 MDELGSALRHSDEPNFRVAPFLFMPDGKLASAISFTILWPILDVQKGDECTRDYLFGIGE 480

Query: 481 EKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVY 540
           +KQRSARLTAWFHTPQNYF+HEYEK+IRNLQSK LA PI QT  NTEG  Q KGGTL VY
Sbjct: 481 DKQRSARLTAWFHTPQNYFVHEYEKYIRNLQSKALALPIPQTTSNTEGRHQIKGGTLRVY 540

Query: 541 TDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVM 600
           TDNPQVEE+L+RPEF+ITSDPKEADIIWTSMQIDEDTKKATGITD+QY+NQFPFEACLVM
Sbjct: 541 TDNPQVEEYLNRPEFIITSDPKEADIIWTSMQIDEDTKKATGITDQQYVNQFPFEACLVM 600

Query: 601 KHHLAETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT 660
           KHHLAET++KAHGC EWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT
Sbjct: 601 KHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT 660

Query: 661 TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR 720
           TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR
Sbjct: 661 TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR 720

Query: 721 LANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSMI 780
           LANNPYSLEKQSLFEYETHFTVMNYRG+LNHKNIA+FVKEFEQEHNVKWLDIHSRVRSMI
Sbjct: 721 LANNPYSLEKQSLFEYETHFTVMNYRGKLNHKNIADFVKEFEQEHNVKWLDIHSRVRSMI 780

Query: 781 RSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEHVFED 840
           RSVFESAA+VHPEMHSPFSRAMYGLDVMLD+SFQPKLLEVTYCPDCTRACKYDVE+VF D
Sbjct: 781 RSVFESAAMVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVENVFGD 840

Query: 841 GVIKGREFYNHVFGCLFLNETTHVTPL 868
           G+IKGREFYN VFGCLFLNETTH TPL
Sbjct: 841 GIIKGREFYNDVFGCLFLNETTHATPL 864

BLAST of MS023133 vs. NCBI nr
Match: KAG6588082.1 (Tubulin--tyrosine ligase-like protein 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1591.6 bits (4120), Expect = 0.0e+00
Identity = 769/868 (88.59%), Postives = 813/868 (93.66%), Query Frame = 0

Query: 1   MAAAGRIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 60
           MAA  RI  YEDFVEVHGLLLAAS LPQSLH QLFQKLTSETFDGGAHFQVE FEDGRCR
Sbjct: 29  MAAGNRIEAYEDFVEVHGLLLAASGLPQSLHCQLFQKLTSETFDGGAHFQVEPFEDGRCR 88

Query: 61  RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 120
           RLVLSSD M+KESHVFLVDHAWTFRLSDAYNQL EV GLAERMASLMCVD+DMNLDE+  
Sbjct: 89  RLVLSSDFMSKESHVFLVDHAWTFRLSDAYNQLLEVPGLAERMASLMCVDVDMNLDED-- 148

Query: 121 VHSNDNEV-KQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLL 180
             + + EV +QSV+EL+ESEI G K+ GN +VRWLELEDLQI+DDTLLSLDL TKFPDLL
Sbjct: 149 --AKEEEVDQQSVWELLESEIHGAKEKGNDSVRWLELEDLQIDDDTLLSLDLPTKFPDLL 208

Query: 181 ALSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEICNSR 240
           ALSLTGNKL NVDVVA+EV+KFKHLRALWLNNNP  ENC E LQQ+VL+G PNLEI NSR
Sbjct: 209 ALSLTGNKLSNVDVVAQEVAKFKHLRALWLNNNPAVENCDEILQQKVLDGSPNLEIYNSR 268

Query: 241 FTHNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPS 300
           FTHNFS+WALGFCGDIYEKDNPGSIYP DHSLQ + +LDLS+RCIHNL NKAFSPVELPS
Sbjct: 269 FTHNFSKWALGFCGDIYEKDNPGSIYPADHSLQDVTSLDLSHRCIHNLNNKAFSPVELPS 328

Query: 301 LSYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGI 360
           LSYLNLRGNPL +NSV DLL ILKEFPCL+SLEVDIPGPLGEKA+DIIESLPNL+TLN I
Sbjct: 329 LSYLNLRGNPLEQNSVGDLLEILKEFPCLSSLEVDIPGPLGEKAVDIIESLPNLHTLNSI 388

Query: 361 DVAKILHSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWY 420
           DV KIL+SGKHVIDSMLLPR PEWTPEEPLPDRVINAMW YLMTYRLADEEKIDETSVWY
Sbjct: 389 DVKKILNSGKHVIDSMLLPRLPEWTPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWY 448

Query: 421 VMDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIG 480
           VMDELGSALRHSD+PNF+VAPFLFMP+GKL SAISFTILWPI +VQKGDECTRDYLFGIG
Sbjct: 449 VMDELGSALRHSDEPNFRVAPFLFMPDGKLASAISFTILWPILDVQKGDECTRDYLFGIG 508

Query: 481 EEKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHV 540
           E+KQRSARLTAWFHTPQNYF+HEYEK+IRNLQSK LASPI QT  NTEG  Q KGGTL V
Sbjct: 509 EDKQRSARLTAWFHTPQNYFVHEYEKYIRNLQSKALASPIPQTTSNTEGRHQIKGGTLRV 568

Query: 541 YTDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLV 600
           YTDNPQVEE+L+RPEF+ITSDPKEADIIWTSMQIDE+TKKATGITD+QY+NQFPFEACLV
Sbjct: 569 YTDNPQVEEYLNRPEFIITSDPKEADIIWTSMQIDEETKKATGITDQQYVNQFPFEACLV 628

Query: 601 MKHHLAETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTID 660
           MKHHLAET++KAHGC EWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTID
Sbjct: 629 MKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTID 688

Query: 661 TTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWV 720
           TTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWV
Sbjct: 689 TTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWV 748

Query: 721 RLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSM 780
           RLANNPYSLEKQSLFEYETHFTVMNYRG+LNHKNIA+FVKEFEQEHNVKWLDIHSRVRSM
Sbjct: 749 RLANNPYSLEKQSLFEYETHFTVMNYRGKLNHKNIADFVKEFEQEHNVKWLDIHSRVRSM 808

Query: 781 IRSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEHVFE 840
           IRSVFESAAVVHPEMHSPFSRAMYGLDVMLD+SFQPKLLEVTYCPDCTRACKYDVE+VF 
Sbjct: 809 IRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVENVFG 868

Query: 841 DGVIKGREFYNHVFGCLFLNETTHVTPL 868
           D +IKGREFYN VFGCLFLNETTH TPL
Sbjct: 869 DEIIKGREFYNDVFGCLFLNETTHATPL 892

BLAST of MS023133 vs. NCBI nr
Match: TYK10026.1 (tubulin--tyrosine ligase-like protein 12 [Cucumis melo var. makuwa])

HSP 1 Score: 1591.6 bits (4120), Expect = 0.0e+00
Identity = 768/871 (88.17%), Postives = 812/871 (93.23%), Query Frame = 0

Query: 1   MAAAGRIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 60
           MA   RI T+EDF +VHGLLL AS LPQSLHRQLFQKLTSETFDGG+HFQVE FEDGRCR
Sbjct: 1   MADGKRIQTFEDFFKVHGLLLTASGLPQSLHRQLFQKLTSETFDGGSHFQVEPFEDGRCR 60

Query: 61  RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 120
           RLVLSSD MAKESHVFLVDHAWTFRLSDAY QL EV GLAERMASLMCVDID+NLDEE+ 
Sbjct: 61  RLVLSSDFMAKESHVFLVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDLNLDEEDE 120

Query: 121 VHS----NDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFP 180
            HS    +D++ KQSV+EL+ESEIRG  + GN +VRWLELEDLQI+DDTLLSLDL TKFP
Sbjct: 121 DHSKSNVDDDDAKQSVWELIESEIRGAIEKGNDSVRWLELEDLQIDDDTLLSLDLPTKFP 180

Query: 181 DLLALSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEIC 240
           DLLALSLTGNKL++VD VAREV KFKHLRALWLN+NPVAENC   LQQRVLEG PNLEI 
Sbjct: 181 DLLALSLTGNKLKDVDTVAREVVKFKHLRALWLNDNPVAENCDASLQQRVLEGSPNLEIY 240

Query: 241 NSRFTHNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVE 300
           NSRFT NFS+WALGFCGD+Y KDNPGSIYP DH+LQCL +LDLS+RCIHNLINKAFSPVE
Sbjct: 241 NSRFTLNFSKWALGFCGDMYGKDNPGSIYPSDHTLQCLTSLDLSSRCIHNLINKAFSPVE 300

Query: 301 LPSLSYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTL 360
           LPSLSYLNLRGNPL +NSV DLL+ILKEFPCL+SLEVDIPGPLGEKA DIIESLPNL+ L
Sbjct: 301 LPSLSYLNLRGNPLEQNSVGDLLKILKEFPCLSSLEVDIPGPLGEKASDIIESLPNLSNL 360

Query: 361 NGIDVAKILHSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETS 420
           NGIDV KIL SGKH+IDSMLLPR PEW PEE LPDRVINAMW YLMTYRLADEEKIDETS
Sbjct: 361 NGIDVEKILSSGKHMIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADEEKIDETS 420

Query: 421 VWYVMDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLF 480
           VWYVMDELGSALRHSD+PNF+VAPFLFMPEG L SAISFTILWPIHNVQKGDECTRDYLF
Sbjct: 421 VWYVMDELGSALRHSDEPNFRVAPFLFMPEGNLTSAISFTILWPIHNVQKGDECTRDYLF 480

Query: 481 GIGEEKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGT 540
           GIGE+KQRSARLTAWFHTPQNYF+HEYEKHI+NLQSKVL SPISQT   TE L+QSKGGT
Sbjct: 481 GIGEDKQRSARLTAWFHTPQNYFVHEYEKHIKNLQSKVLTSPISQTTSKTEELRQSKGGT 540

Query: 541 LHVYTDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEA 600
           L VYTDNPQVEEFL+RPEF ITSDPKEADIIWTSMQIDEDT+KATGITD+QY+NQFPFEA
Sbjct: 541 LRVYTDNPQVEEFLNRPEFTITSDPKEADIIWTSMQIDEDTRKATGITDKQYVNQFPFEA 600

Query: 601 CLVMKHHLAETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMAR 660
           CLVMKHHLAETI+KAHGC EWLQPTYNLETHLSQLIGDY VRKRDGLNNLWILKPWNMAR
Sbjct: 601 CLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKRDGLNNLWILKPWNMAR 660

Query: 661 TIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADS 720
           TIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADS
Sbjct: 661 TIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADS 720

Query: 721 FWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRV 780
           FWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIA+FV+EFEQEHNVKWLDIHSRV
Sbjct: 721 FWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIADFVREFEQEHNVKWLDIHSRV 780

Query: 781 RSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEH 840
            SMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLD+SFQPKLLEVTYCPDCTRACKYDVE+
Sbjct: 781 TSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVEN 840

Query: 841 VFEDGVIKGREFYNHVFGCLFLNETTHVTPL 868
           VF  GVI+GREFYN++FGCLFLNETTHVTPL
Sbjct: 841 VFGGGVIEGREFYNYIFGCLFLNETTHVTPL 871

BLAST of MS023133 vs. ExPASy Swiss-Prot
Match: Q3UDE2 (Tubulin--tyrosine ligase-like protein 12 OS=Mus musculus OX=10090 GN=Ttll12 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.1e-82
Identity = 188/522 (36.02%), Postives = 276/522 (52.87%), Query Frame = 0

Query: 382 EWTPEEPLPD---RVINAMWLYLMTYRLADEEKIDETSVWYVMDELGSALRHSDQPNFKV 441
           E+  E P P+    V+  MW +  TY+LA     ++  VWY+MDE GS ++HSD P+F  
Sbjct: 147 EFHGEVPSPEVVALVLEEMWKFNQTYQLAHGTAEEKVPVWYIMDEFGSRIQHSDMPSFAT 206

Query: 442 APFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGEEKQRSARLTAW------- 501
           APF +MP+      +++T+LWP+ ++  G+E TRD+ +G  +   R   L  W       
Sbjct: 207 APFFYMPQ-----QVAYTLLWPLRDLDTGEEVTRDFAYGEADPLIRKCMLLPWAPADMLD 266

Query: 502 --FHTPQ---NYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVYTDNPQV 561
             F TP+    Y+    E++   L   +  SP++          + +G    V+ D  QV
Sbjct: 267 LSFSTPEPPAKYYQAILEENKEKLPLAI--SPVA----------RPQGHVFRVHCDVQQV 326

Query: 562 EEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVMKHHLAE 621
              L  P F  T    +ADI +      +D  K +  + +  +NQFP E  L +K  LA 
Sbjct: 327 LGHLTHPRFTFTDSEADADIFFHFSHF-KDYMKLSQESPQVLLNQFPCENLLTVKDCLAS 386

Query: 622 TIQKAHG--CAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDTTVTD 681
             ++A G     WL  T+NL T L Q +  +  R+R G +N WI KPWN+AR++DT VT+
Sbjct: 387 IARRAGGPEGPPWLPRTFNLRTELPQFVSYFQHRERRGEDNHWICKPWNLARSLDTHVTN 446

Query: 682 NLSAIIRLMETGPKICQKYIEHPALF-----NGKKFDLRYIVLVRSMKPLEIFLADSFWV 741
           NL +IIR  E+ PK+  KYIE P LF        KFD+RYIVL+RS++PL +F  D FW+
Sbjct: 447 NLHSIIRHRESTPKVVSKYIESPVLFLREDVGNVKFDIRYIVLLRSVRPLRLFAYDVFWL 506

Query: 742 RLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNI--AEFVKEFEQEH-NVKWLDIHSRV 801
           R +N P++L+   L +YE HFTVMNY   +  K +   EF+ +FE+++    W D+ + +
Sbjct: 507 RFSNRPFALD--DLDDYEKHFTVMNYDPDVVLKQVHYNEFIPQFEKQYPEFPWSDVQAEI 566

Query: 802 RSMIRSVFESAAVVHPEM---HSPFSRAMYGLDVMLD--------ASFQPKLLEVTYCPD 861
                 +F+ A    P M     P SRAMY +D+ML+           QP++LEV + PD
Sbjct: 567 FKAFTELFQVACAKPPPMGLCDYPSSRAMYAIDLMLNWDNHPDGKRVMQPQILEVNFNPD 626

Query: 862 CTRACKYDVEHVFEDGVIKGREFYNHVFGCLFLNET--THVT 866
           C RAC+Y               F+N VF  LFL+ET   HVT
Sbjct: 627 CERACRYH------------PSFFNDVFSTLFLDETDNCHVT 636

BLAST of MS023133 vs. ExPASy Swiss-Prot
Match: Q14166 (Tubulin--tyrosine ligase-like protein 12 OS=Homo sapiens OX=9606 GN=TTLL12 PE=1 SV=2)

HSP 1 Score: 297.0 bits (759), Expect = 7.1e-79
Identity = 178/498 (35.74%), Postives = 262/498 (52.61%), Query Frame = 0

Query: 393 VINAMWLYLMTYRLADEEKIDETSVWYVMDELGSALRHSDQPNFKVAPFLFMPEGKLESA 452
           V+  MW +  TY+LA     ++  VWY+MDE GS ++H+D P+F  APF +MP+      
Sbjct: 166 VLEEMWKFNQTYQLAHGTAEEKMPVWYIMDEFGSRIQHADVPSFATAPFFYMPQ-----Q 225

Query: 453 ISFTILWPIHNVQKGDECTRDYLFGIGEEKQRSARLTAWFHTPQNYFMHEYEKHIRNLQS 512
           +++T+LWP+ ++  G+E TRD+ +G  +   R   L  W  T          +       
Sbjct: 226 VAYTLLWPLRDLDTGEEVTRDFAYGETDPLIRKCMLLPWAPTDMLDLSSCTPEPPAEHYQ 285

Query: 513 KVLASPISQTMVNTEGLQQSKGGTLHVYTDNPQVEEFLDRPEFVITSDPKEADIIWTSMQ 572
            +L     +  ++   +    G    VYTD  QV   L  P F +T    +ADI++    
Sbjct: 286 AILEENKEKLPLDINPVVHPHGHIFKVYTDVQQVASSLTHPRFTLTQSEADADILFNFSH 345

Query: 573 IDEDTKKATGITDEQYINQFPFEACLVMKHHLAETIQKAHG--CAEWLQPTYNLETHLSQ 632
             +D +K +       +NQFP E  L +K  LA   ++A G     WL  T+NL T L Q
Sbjct: 346 F-KDYRKLSQERPGVLLNQFPCENLLTVKDCLASIARRAGGPEGPPWLPRTFNLRTELPQ 405

Query: 633 LIGDYLVRKRDGLNNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALF 692
            +  +  R+R G +N WI KPWN+AR++DT VT +L +IIR  E+ PK+  KYIE P LF
Sbjct: 406 FVSYFQQRERWGEDNHWICKPWNLARSLDTHVTKSLHSIIRHRESTPKVVSKYIESPVLF 465

Query: 693 ----NGK-KFDLRYIVLVRSMKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNY 752
                GK KFD+RYIVL+RS++PL +F+ D FW+R +N  ++L    L +YE HFTVMNY
Sbjct: 466 LREDVGKVKFDIRYIVLLRSVRPLRLFVYDVFWLRFSNRAFAL--NDLDDYEKHFTVMNY 525

Query: 753 RGRLNHKNI--AEFVKEFEQEH-NVKWLDIHSRVRSMIRSVFESAAVVHPEM---HSPFS 812
              +  K +   EF+ EFE+++    W D+ + +      +F+ A    P +     P S
Sbjct: 526 DPDVVLKQVHCEEFIPEFEKQYPEFPWTDVQAEIFRAFTELFQVACAKPPPLGLCDYPSS 585

Query: 813 RAMYGLDVMLD--------ASFQPKLLEVTYCPDCTRACKYDVEHVFEDGVIKGREFYNH 868
           RAMY +D+ML            QP++LEV + PDC RAC+Y               F+N 
Sbjct: 586 RAMYAVDLMLKWDNGPDGRRVMQPQILEVNFNPDCERACRYH------------PTFFND 643

HSP 2 Score: 70.1 bits (170), Expect = 1.4e-10
Identity = 44/134 (32.84%), Postives = 66/134 (49.25%), Query Frame = 0

Query: 12  DFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHF---QVETFEDGR---------- 71
           +F  +HG  L AS +P+    +L  KL  E FD G  F   QVE  E+            
Sbjct: 30  EFAALHGPALRASGVPERYWGRLLHKLEHEVFDAGEVFGIMQVEEVEEEEDEAAREVRKQ 89

Query: 72  --------CRRLVLSSDSMAKESH---VFLVDHAWTFRLSDAYNQLKEVSGLAERMASLM 122
                   C +++++ +S  + +H   +FL+DHAWT R+  A  QL++V GL  RMA+LM
Sbjct: 90  QPNPGNELCYKVIVTRESGLQAAHPNSIFLIDHAWTCRVEHARQQLQQVPGLLHRMANLM 149

BLAST of MS023133 vs. ExPASy Swiss-Prot
Match: Q09512 (Tubulin--tyrosine ligase-like protein 12 OS=Caenorhabditis elegans OX=6239 GN=ttll-12 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 8.7e-69
Identity = 168/520 (32.31%), Postives = 265/520 (50.96%), Query Frame = 0

Query: 385 PEEPLPDRVINAMWLYLMTYRLA---DEEKIDETSVWYVMDELGSALRHSDQPNFKVAPF 444
           P+  L ++V+ A+W Y  TY ++   D  +I++ SVWYVMD+ G+ +RHS +PN ++ P 
Sbjct: 168 PKNELTEKVLKALWKYSQTYSVSYQLDNGEIEKKSVWYVMDDFGTRVRHSTEPNVRIVPL 227

Query: 445 LFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGEEKQRSARLTAW-FHTPQNYFM 504
           +F+P+       +++I++    V   +E   D+   +      +A+   W  +  Q +  
Sbjct: 228 MFLPQN-----CAYSIMFLTKPVNTDEEIMMDWASNV-----ITAQHPEWRKYIEQPWAA 287

Query: 505 HEYEKH------------IRNLQSKVLASPISQTMVNT---EGLQQSKGGTLHVYTDNPQ 564
            ++ K                     LA P  Q    +     L   K   + VY D+ Q
Sbjct: 288 QDFSKETMIPDAPTLEYFTSGRNPDFLAGPTEQQTCQSAIFTSLPILKKRKIKVYADDTQ 347

Query: 565 VEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVMKHHLA 624
           + E L   +     D K+AD+IW       D K+ +       INQFPFE+C+ +K  LA
Sbjct: 348 LTEHLKNHKVEYVDDIKKADVIWMIKHF-HDYKQLSEENPCGMINQFPFESCITVKDLLA 407

Query: 625 ETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDTTVTDN 684
               +     +W Q TYNL T L + +  +  R+ +G +N+WI+KPWN+AR +D TVT++
Sbjct: 408 ACAMRDPAKNDWYQLTYNLNTQLPEFVARFQNRELNGQHNVWIVKPWNLARGMDMTVTED 467

Query: 685 LSAIIRLMETGPKICQKYIEHPALF------NGKKFDLRYIVLVRSMKPLEIFLADSFWV 744
           L+ IIR++ETGPKI  +YI  P LF      N  KFDLRYIV +  + P+  ++ + FW+
Sbjct: 468 LNQIIRMIETGPKIVCEYIPRPLLFPRPDNGNKVKFDLRYIVFLNGIAPVTAYVYNRFWI 527

Query: 745 RLANNPYSLEKQSLFEYETHFTVMNY--RGRLNHKNIAEFVKEFEQEH-NVKWLDIHSRV 804
           R A N +SL   +  + ETHFTV NY  + ++       F++  E+ +  ++W ++   +
Sbjct: 528 RFAINEFSL--SNFEDVETHFTVFNYLDKEKILQMKCENFIETIEKAYPRIQWSEVQKDI 587

Query: 805 RSMIRSVFESAAVVH-PEMHSP--FSRAMYGLDVMLDAS----FQPKLLEVTYCPDCTRA 864
              IR   E+AA    P   +P   SRAMYG+D+ML        +  LLE+ + PD TRA
Sbjct: 588 NLTIRKAIEAAAKEEAPRGVAPNVQSRAMYGVDIMLQHGDNDVIKSTLLEINFMPDTTRA 647

Query: 865 CKYDVEHVFEDGVIKGREFYNHVFGCLFLNE--TTHVTPL 868
           C+Y              +F + VF  LFL+E   T VTP+
Sbjct: 648 CQY------------YPDFADTVFETLFLDEIDPTKVTPI 662

BLAST of MS023133 vs. ExPASy Swiss-Prot
Match: A8XXC0 (Tubulin--tyrosine ligase-like protein 12 OS=Caenorhabditis briggsae OX=6238 GN=ttll-12 PE=3 SV=2)

HSP 1 Score: 256.5 bits (654), Expect = 1.1e-66
Identity = 167/523 (31.93%), Postives = 270/523 (51.63%), Query Frame = 0

Query: 385 PEEPLPDRVINAMWLYLMTYRLA---DEEKIDETSVWYVMDELGSALRHSDQPNFKVAPF 444
           P++ + +RV+  +W +  TY ++   +  ++++  VWYVMD+ GS +RHS  PN ++ P 
Sbjct: 171 PKKTMTERVMRNLWKFAQTYTVSYQLENGEMEKKHVWYVMDDFGSRIRHSGCPNVRIVPL 230

Query: 445 LFMPEGKLESAI----------SFTILWPIHNVQKGDECTRDYLFGIGEEKQRSAR---- 504
           +F+P+    S +            T+ W  + +   +   R YL     EK  S+     
Sbjct: 231 MFLPQNCAYSIMFLTKPVKIDDEITMDWAANVITAKNPEWRQYLEMPWAEKDFSSESMVP 290

Query: 505 ---LTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVYTDNP 564
                 +F + +N      EK  +  +S + +   + +++  +G  +     L ++ D+ 
Sbjct: 291 GPPTLEYFTSGRNPDFLADEKDKKTCESAIFS---ALSVLKKQGKIKI---FLQIFADDT 350

Query: 565 QVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVMKHHL 624
           Q+ E L   +     D K AD+IW      + +  A      Q INQFPFE+C+ +K  L
Sbjct: 351 QLTEHLKSRQIEYVDDWKAADVIWMIKHFHDYSNLAQENPCAQ-INQFPFESCITVKDLL 410

Query: 625 AETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDTTVTD 684
           A    +     +W Q TYNL T L + +  +  R+++G +N+WI+KPWN+AR ++  VTD
Sbjct: 411 AACAMRDPSKNDWYQLTYNLNTQLPEFVARFQNRQKNGEHNVWIVKPWNLARGMEMAVTD 470

Query: 685 NLSAIIRLMETGPKICQKYIEHPALF------NGKKFDLRYIVLVRSMKPLEIFLADSFW 744
           +L+ IIR++ETGPKI  +YI  P LF      N  KFDLRYIV V ++ P+  ++ + FW
Sbjct: 471 DLNQIIRMVETGPKIVCEYIARPLLFPRPDNGNKVKFDLRYIVFVNAIGPVTAYVYNRFW 530

Query: 745 VRLANNPYSLEKQSLFEYETHFTVMNY--RGRLNHKNIAEFVKEFEQEH-NVKWLDIHSR 804
           +R A N + L      + ETHFTV NY  + ++      +FV+  E+ +  +KW  I + 
Sbjct: 531 IRFAINQFQLGAYD--DLETHFTVFNYLDKEKVLQMKCEKFVEIIEKTYPKLKWTQIQAD 590

Query: 805 VRSMIRSVFE-SAAVVHPEMHSP--FSRAMYGLDVMLDAS------FQPKLLEVTYCPDC 864
           + S IR   E +A+   P   +P   SRAMYG+D+ML  S       +P LLE+ + PD 
Sbjct: 591 INSTIRKAIEVAASEPSPRGVAPNTQSRAMYGVDIMLQESPESPDVIKPTLLEINFMPDT 650

Query: 865 TRACKYDVEHVFEDGVIKGREFYNHVFGCLFLNE--TTHVTPL 868
           TRAC+Y              +F + VF  +FL+E   T VTP+
Sbjct: 651 TRACQY------------YPDFADTVFDTMFLDEIDPTKVTPI 672

BLAST of MS023133 vs. ExPASy Swiss-Prot
Match: Q23K29 (Tubulin glycylase 3D OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTLL3D PE=3 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 3.6e-14
Identity = 67/218 (30.73%), Postives = 103/218 (47.25%), Query Frame = 0

Query: 642 GLNNLWILKPWNMARTIDTTVTDNLSAII---RLMETGPKICQKYIEHPALFNGKKFDLR 701
           G +N+WI+KP  ++R    T   NL  II   + ME    I QKYIE+P L   +KFD+R
Sbjct: 700 GEDNIWIVKPAGLSRGRGITCYKNLVEIIDHAKSMEL-QMIVQKYIENPVLIKQRKFDIR 759

Query: 702 YIVLVRSMKPLEIFLADSFWVRLANNPYSLEKQS-LFEYETHFTVMNYRGRLNHKNIAEF 761
             VLV    PL I+  D  +VR + + YS +  S  F++ T+  +   + +     I   
Sbjct: 760 IWVLVTDWNPLAIWYFDECYVRFSADSYSTKNLSNKFQHLTNNAISKKKAQQGQDEITLQ 819

Query: 762 VKEFEQE-----------HNVKWLDIHSRVRSMIR-SVFESAAVVHPEMHSPFSRAMYGL 821
              + QE           +NV    I  ++ ++I+ S+   +  V    +   S  ++G 
Sbjct: 820 GNMYTQEQLENFFIETEGYNVFQQKIKPQIINIIKWSILSCSDTVESRKN---SMELFGY 879

Query: 822 DVMLDASFQPKLLEVTYCPD---CTRACKYDVEHVFED 841
           D+M+D +F P LLEV   P     T   K  V+ V ED
Sbjct: 880 DIMIDTNFNPWLLEVNTSPSLEYSTEITKKLVKQVLED 913

BLAST of MS023133 vs. ExPASy TrEMBL
Match: A0A6J1D0T0 (tubulin--tyrosine ligase-like protein 12 OS=Momordica charantia OX=3673 GN=LOC111016537 PE=4 SV=1)

HSP 1 Score: 1780.4 bits (4610), Expect = 0.0e+00
Identity = 862/867 (99.42%), Postives = 863/867 (99.54%), Query Frame = 0

Query: 1   MAAAGRIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 60
           MAAAGRIGTYEDFVEVHGLLLAAS LPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR
Sbjct: 78  MAAAGRIGTYEDFVEVHGLLLAASGLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 137

Query: 61  RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 120
           RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG
Sbjct: 138 RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 197

Query: 121 VHSNDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLLA 180
           VHSNDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLLA
Sbjct: 198 VHSNDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLLA 257

Query: 181 LSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEICNSRF 240
           LSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEI NSRF
Sbjct: 258 LSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEIYNSRF 317

Query: 241 THNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPSL 300
           THNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPSL
Sbjct: 318 THNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPSL 377

Query: 301 SYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGID 360
           SYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGID
Sbjct: 378 SYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGID 437

Query: 361 VAKILHSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWYV 420
           VAKILHSGKHVIDSML PRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWYV
Sbjct: 438 VAKILHSGKHVIDSMLRPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWYV 497

Query: 421 MDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGE 480
           MDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGE
Sbjct: 498 MDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGE 557

Query: 481 EKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVY 540
           EKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVY
Sbjct: 558 EKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVY 617

Query: 541 TDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVM 600
           TDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVM
Sbjct: 618 TDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVM 677

Query: 601 KHHLAETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT 660
           KHHLAETIQKAHGCAEWLQPTYNLE HLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT
Sbjct: 678 KHHLAETIQKAHGCAEWLQPTYNLEIHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT 737

Query: 661 TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR 720
           TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR
Sbjct: 738 TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR 797

Query: 721 LANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSMI 780
           LANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSMI
Sbjct: 798 LANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSMI 857

Query: 781 RSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEHVFED 840
           RSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVE+VFED
Sbjct: 858 RSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVENVFED 917

Query: 841 GVIKGREFYNHVFGCLFLNETTHVTPL 868
           GVIKGREFYNHVFGCLFLNETTHVTPL
Sbjct: 918 GVIKGREFYNHVFGCLFLNETTHVTPL 944

BLAST of MS023133 vs. ExPASy TrEMBL
Match: A0A6J1F3Y4 (tubulin--tyrosine ligase-like protein 12 OS=Cucurbita moschata OX=3662 GN=LOC111439992 PE=4 SV=1)

HSP 1 Score: 1597.4 bits (4135), Expect = 0.0e+00
Identity = 772/868 (88.94%), Postives = 815/868 (93.89%), Query Frame = 0

Query: 1   MAAAGRIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 60
           MAA  RI  YEDFVEVHGLLLAAS LPQSLHRQLFQKLTSETFDGGAHFQVE FEDGRCR
Sbjct: 1   MAAGNRIEAYEDFVEVHGLLLAASGLPQSLHRQLFQKLTSETFDGGAHFQVEPFEDGRCR 60

Query: 61  RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 120
           RLVLSSD M+KESHVFLVDHAWTFRLSDAYNQL EV GLAERMASLMCVD+DMNLDE+  
Sbjct: 61  RLVLSSDFMSKESHVFLVDHAWTFRLSDAYNQLLEVPGLAERMASLMCVDVDMNLDED-- 120

Query: 121 VHSNDNEV-KQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLL 180
             + + EV +QSV+EL+ESEI G K+ GNG+VRWLELEDLQI+DDTLLSLDL TKFPDLL
Sbjct: 121 --AKEEEVDQQSVWELLESEIHGAKEKGNGSVRWLELEDLQIDDDTLLSLDLPTKFPDLL 180

Query: 181 ALSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEICNSR 240
           ALSLTGNKL NVDVVA+EV+KFKHLRALWLNNNP  ENC E LQQ+VL+G PNLEI NSR
Sbjct: 181 ALSLTGNKLSNVDVVAQEVAKFKHLRALWLNNNPAVENCDEILQQKVLDGSPNLEIYNSR 240

Query: 241 FTHNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPS 300
           FTHNFSEWALGFCGDIYEKDNPGSIYP DHSLQ + +LDLS+R IHNL NKAFSPVELPS
Sbjct: 241 FTHNFSEWALGFCGDIYEKDNPGSIYPADHSLQDVTSLDLSHRGIHNLNNKAFSPVELPS 300

Query: 301 LSYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGI 360
           LSYLNLRGNPL +NSV DLL ILKEFPCL+SLEVDIPGPLGEKA+DIIESLPNL+TLN I
Sbjct: 301 LSYLNLRGNPLEQNSVGDLLEILKEFPCLSSLEVDIPGPLGEKAVDIIESLPNLHTLNSI 360

Query: 361 DVAKILHSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWY 420
           DV KIL+SGKHVIDSMLLPR PEWTPEEPLPDRVINAMW YLMTYRLADEEKIDETSVWY
Sbjct: 361 DVKKILNSGKHVIDSMLLPRLPEWTPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWY 420

Query: 421 VMDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIG 480
           VMDELGSALRHSD+PNF+VAPFLFMP+GKL SAISFTILWPI +VQKGDECTRDYLFGIG
Sbjct: 421 VMDELGSALRHSDEPNFRVAPFLFMPDGKLASAISFTILWPILDVQKGDECTRDYLFGIG 480

Query: 481 EEKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHV 540
           E+KQRSARLTAWFHTPQNYF+HEYEK+IRNLQSK LASPI QT  NTEG  Q KGGTL V
Sbjct: 481 EDKQRSARLTAWFHTPQNYFVHEYEKYIRNLQSKALASPIPQTTSNTEGRHQIKGGTLRV 540

Query: 541 YTDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLV 600
           YTDNPQVEE+L+RPEF+ITSDPKEADIIWTSMQIDE+TKKATGITD+QY+NQFPFEACLV
Sbjct: 541 YTDNPQVEEYLNRPEFIITSDPKEADIIWTSMQIDEETKKATGITDQQYVNQFPFEACLV 600

Query: 601 MKHHLAETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTID 660
           MKHHLAET++KAHGC EWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTID
Sbjct: 601 MKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTID 660

Query: 661 TTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWV 720
           TTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWV
Sbjct: 661 TTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWV 720

Query: 721 RLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSM 780
           RLANNPYSLEKQSLFEYETHFTVMNYRG+LNHKNIA+FVKEFEQEHNVKWLDIHSRVRSM
Sbjct: 721 RLANNPYSLEKQSLFEYETHFTVMNYRGKLNHKNIADFVKEFEQEHNVKWLDIHSRVRSM 780

Query: 781 IRSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEHVFE 840
           IRSVFESAAVVHPEMHSPFSRAMYGLDVMLD+SFQPKLLEVTYCPDCTRACKYDVE+VF 
Sbjct: 781 IRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVENVFG 840

Query: 841 DGVIKGREFYNHVFGCLFLNETTHVTPL 868
           DG+IKGREFYN VFGCLFLNETTH TPL
Sbjct: 841 DGIIKGREFYNDVFGCLFLNETTHATPL 864

BLAST of MS023133 vs. ExPASy TrEMBL
Match: A0A6J1HSW5 (tubulin--tyrosine ligase-like protein 12 OS=Cucurbita maxima OX=3661 GN=LOC111466337 PE=4 SV=1)

HSP 1 Score: 1594.7 bits (4128), Expect = 0.0e+00
Identity = 770/867 (88.81%), Postives = 810/867 (93.43%), Query Frame = 0

Query: 1   MAAAGRIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 60
           MAA  RI  YEDFVEVHGLLLAAS LPQSLHRQLFQKLTSETFDGGAHFQVE FEDGRCR
Sbjct: 1   MAAGNRIEAYEDFVEVHGLLLAASGLPQSLHRQLFQKLTSETFDGGAHFQVEPFEDGRCR 60

Query: 61  RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 120
           RLVLSSD MAKESHVFLVDHAWTFRLSDAYNQL EV GLAERMASLMCVD+DMNLDE+  
Sbjct: 61  RLVLSSDFMAKESHVFLVDHAWTFRLSDAYNQLLEVPGLAERMASLMCVDVDMNLDEDVK 120

Query: 121 VHSNDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLLA 180
               D   +QSV+EL+ESEI G K+ GNG+VRWLELEDLQI+DDTLLSLDL TKFPDLLA
Sbjct: 121 EEEVD---QQSVWELLESEIHGAKEEGNGSVRWLELEDLQIDDDTLLSLDLPTKFPDLLA 180

Query: 181 LSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEICNSRF 240
           LSLTGNKL NVDVVA+EV+KFKHLRALWLNNNP  ENC E LQQ+VL+G PNLEI NSRF
Sbjct: 181 LSLTGNKLSNVDVVAQEVAKFKHLRALWLNNNPAVENCDEILQQKVLDGSPNLEIYNSRF 240

Query: 241 THNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPSL 300
           THNFSEWALGFCGDIYEKDNPGSIYP DHSLQ + +LDLS+RCIHNL NKAFSPVELPSL
Sbjct: 241 THNFSEWALGFCGDIYEKDNPGSIYPADHSLQDVTSLDLSHRCIHNLNNKAFSPVELPSL 300

Query: 301 SYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGID 360
           SYLNLRGNPL +NSV DLL ILK FPCL+SLEVDIPGPLGEKA+DIIESLPNL+TLN ID
Sbjct: 301 SYLNLRGNPLEQNSVGDLLEILKGFPCLSSLEVDIPGPLGEKAVDIIESLPNLHTLNSID 360

Query: 361 VAKILHSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWYV 420
           V KIL+SGKHVIDSMLLPR PEWTPEEPL DRVINAMW YLMTYRLADEEKIDETSVWYV
Sbjct: 361 VKKILNSGKHVIDSMLLPRLPEWTPEEPLRDRVINAMWQYLMTYRLADEEKIDETSVWYV 420

Query: 421 MDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGE 480
           MDELGSALRHSD+PNF+VAPFLFMP+GKL SAISFTILWPI +VQKGDECTRDYLFGIGE
Sbjct: 421 MDELGSALRHSDEPNFRVAPFLFMPDGKLASAISFTILWPILDVQKGDECTRDYLFGIGE 480

Query: 481 EKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVY 540
           +KQRSARLTAWFHTPQNYF+HEYEK+IRNLQSK LA PI QT  NTEG  Q KGGTL VY
Sbjct: 481 DKQRSARLTAWFHTPQNYFVHEYEKYIRNLQSKALALPIPQTTSNTEGRHQIKGGTLRVY 540

Query: 541 TDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVM 600
           TDNPQVEE+L+RPEF+ITSDPKEADIIWTSMQIDEDTKKATGITD+QY+NQFPFEACLVM
Sbjct: 541 TDNPQVEEYLNRPEFIITSDPKEADIIWTSMQIDEDTKKATGITDQQYVNQFPFEACLVM 600

Query: 601 KHHLAETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT 660
           KHHLAET++KAHGC EWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT
Sbjct: 601 KHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDT 660

Query: 661 TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR 720
           TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR
Sbjct: 661 TVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVR 720

Query: 721 LANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSMI 780
           LANNPYSLEKQSLFEYETHFTVMNYRG+LNHKNIA+FVKEFEQEHNVKWLDIHSRVRSMI
Sbjct: 721 LANNPYSLEKQSLFEYETHFTVMNYRGKLNHKNIADFVKEFEQEHNVKWLDIHSRVRSMI 780

Query: 781 RSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEHVFED 840
           RSVFESAA+VHPEMHSPFSRAMYGLDVMLD+SFQPKLLEVTYCPDCTRACKYDVE+VF D
Sbjct: 781 RSVFESAAMVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVENVFGD 840

Query: 841 GVIKGREFYNHVFGCLFLNETTHVTPL 868
           G+IKGREFYN VFGCLFLNETTH TPL
Sbjct: 841 GIIKGREFYNDVFGCLFLNETTHATPL 864

BLAST of MS023133 vs. ExPASy TrEMBL
Match: A0A5D3CI02 (Tubulin--tyrosine ligase-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001580 PE=4 SV=1)

HSP 1 Score: 1591.6 bits (4120), Expect = 0.0e+00
Identity = 768/871 (88.17%), Postives = 812/871 (93.23%), Query Frame = 0

Query: 1   MAAAGRIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 60
           MA   RI T+EDF +VHGLLL AS LPQSLHRQLFQKLTSETFDGG+HFQVE FEDGRCR
Sbjct: 1   MADGKRIQTFEDFFKVHGLLLTASGLPQSLHRQLFQKLTSETFDGGSHFQVEPFEDGRCR 60

Query: 61  RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 120
           RLVLSSD MAKESHVFLVDHAWTFRLSDAY QL EV GLAERMASLMCVDID+NLDEE+ 
Sbjct: 61  RLVLSSDFMAKESHVFLVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDLNLDEEDE 120

Query: 121 VHS----NDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFP 180
            HS    +D++ KQSV+EL+ESEIRG  + GN +VRWLELEDLQI+DDTLLSLDL TKFP
Sbjct: 121 DHSKSNVDDDDAKQSVWELIESEIRGAIEKGNDSVRWLELEDLQIDDDTLLSLDLPTKFP 180

Query: 181 DLLALSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEIC 240
           DLLALSLTGNKL++VD VAREV KFKHLRALWLN+NPVAENC   LQQRVLEG PNLEI 
Sbjct: 181 DLLALSLTGNKLKDVDTVAREVVKFKHLRALWLNDNPVAENCDASLQQRVLEGSPNLEIY 240

Query: 241 NSRFTHNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVE 300
           NSRFT NFS+WALGFCGD+Y KDNPGSIYP DH+LQCL +LDLS+RCIHNLINKAFSPVE
Sbjct: 241 NSRFTLNFSKWALGFCGDMYGKDNPGSIYPSDHTLQCLTSLDLSSRCIHNLINKAFSPVE 300

Query: 301 LPSLSYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTL 360
           LPSLSYLNLRGNPL +NSV DLL+ILKEFPCL+SLEVDIPGPLGEKA DIIESLPNL+ L
Sbjct: 301 LPSLSYLNLRGNPLEQNSVGDLLKILKEFPCLSSLEVDIPGPLGEKASDIIESLPNLSNL 360

Query: 361 NGIDVAKILHSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETS 420
           NGIDV KIL SGKH+IDSMLLPR PEW PEE LPDRVINAMW YLMTYRLADEEKIDETS
Sbjct: 361 NGIDVEKILSSGKHMIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADEEKIDETS 420

Query: 421 VWYVMDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLF 480
           VWYVMDELGSALRHSD+PNF+VAPFLFMPEG L SAISFTILWPIHNVQKGDECTRDYLF
Sbjct: 421 VWYVMDELGSALRHSDEPNFRVAPFLFMPEGNLTSAISFTILWPIHNVQKGDECTRDYLF 480

Query: 481 GIGEEKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGT 540
           GIGE+KQRSARLTAWFHTPQNYF+HEYEKHI+NLQSKVL SPISQT   TE L+QSKGGT
Sbjct: 481 GIGEDKQRSARLTAWFHTPQNYFVHEYEKHIKNLQSKVLTSPISQTTSKTEELRQSKGGT 540

Query: 541 LHVYTDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEA 600
           L VYTDNPQVEEFL+RPEF ITSDPKEADIIWTSMQIDEDT+KATGITD+QY+NQFPFEA
Sbjct: 541 LRVYTDNPQVEEFLNRPEFTITSDPKEADIIWTSMQIDEDTRKATGITDKQYVNQFPFEA 600

Query: 601 CLVMKHHLAETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMAR 660
           CLVMKHHLAETI+KAHGC EWLQPTYNLETHLSQLIGDY VRKRDGLNNLWILKPWNMAR
Sbjct: 601 CLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKRDGLNNLWILKPWNMAR 660

Query: 661 TIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADS 720
           TIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADS
Sbjct: 661 TIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADS 720

Query: 721 FWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRV 780
           FWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIA+FV+EFEQEHNVKWLDIHSRV
Sbjct: 721 FWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIADFVREFEQEHNVKWLDIHSRV 780

Query: 781 RSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEH 840
            SMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLD+SFQPKLLEVTYCPDCTRACKYDVE+
Sbjct: 781 TSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVEN 840

Query: 841 VFEDGVIKGREFYNHVFGCLFLNETTHVTPL 868
           VF  GVI+GREFYN++FGCLFLNETTHVTPL
Sbjct: 841 VFGGGVIEGREFYNYIFGCLFLNETTHVTPL 871

BLAST of MS023133 vs. ExPASy TrEMBL
Match: A0A5A7UQ89 (Tubulin--tyrosine ligase-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001610 PE=4 SV=1)

HSP 1 Score: 1589.7 bits (4115), Expect = 0.0e+00
Identity = 767/871 (88.06%), Postives = 812/871 (93.23%), Query Frame = 0

Query: 1   MAAAGRIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCR 60
           MA   RI T+EDF +VHGLLL AS LPQSLHRQLFQKLTSETFDGG+HFQVE FEDGRCR
Sbjct: 1   MADGKRIQTFEDFFKVHGLLLTASGLPQSLHRQLFQKLTSETFDGGSHFQVEPFEDGRCR 60

Query: 61  RLVLSSDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEG 120
           RLVLSSD MAKESHVFLVDHAWTFRLSDAY QL EV GLAERMASLMCVDID+NLDEE+ 
Sbjct: 61  RLVLSSDFMAKESHVFLVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDLNLDEEDE 120

Query: 121 VHS----NDNEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFP 180
            HS    +D++ KQSV+EL+ESEIRG  + GN +VRWLELE+LQI+DDTLLSLDL TKFP
Sbjct: 121 DHSKSNVDDDDAKQSVWELIESEIRGAIEKGNDSVRWLELENLQIDDDTLLSLDLPTKFP 180

Query: 181 DLLALSLTGNKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEIC 240
           DLLALSLTGNKL++VD VAREV KFKHLRALWLN+NPVAENC   LQQRVLEG PNLEI 
Sbjct: 181 DLLALSLTGNKLKDVDTVAREVVKFKHLRALWLNDNPVAENCDASLQQRVLEGSPNLEIY 240

Query: 241 NSRFTHNFSEWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVE 300
           NSRFT NFS+WALGFCGD+Y KDNPGSIYP DH+LQCL +LDLS+RCIHNLINKAFSPVE
Sbjct: 241 NSRFTLNFSKWALGFCGDMYGKDNPGSIYPSDHTLQCLTSLDLSSRCIHNLINKAFSPVE 300

Query: 301 LPSLSYLNLRGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTL 360
           LPSLSYLNLRGNPL +NSV DLL+ILKEFPCL+SLEVDIPGPLGEKA DIIESLPNL+ L
Sbjct: 301 LPSLSYLNLRGNPLEQNSVGDLLKILKEFPCLSSLEVDIPGPLGEKASDIIESLPNLSNL 360

Query: 361 NGIDVAKILHSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETS 420
           NGIDV KIL SGKH+IDSMLLPR PEW PEE LPDRVINAMW YLMTYRLADEEKIDETS
Sbjct: 361 NGIDVEKILSSGKHMIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADEEKIDETS 420

Query: 421 VWYVMDELGSALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLF 480
           VWYVMDELGSALRHSD+PNF+VAPFLFMPEG L SAISFTILWPIHNVQKGDECTRDYLF
Sbjct: 421 VWYVMDELGSALRHSDEPNFRVAPFLFMPEGNLTSAISFTILWPIHNVQKGDECTRDYLF 480

Query: 481 GIGEEKQRSARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGT 540
           GIGE+KQRSARLTAWFHTPQNYF+HEYEKHI+NLQSKVL SPISQT   TE L+QSKGGT
Sbjct: 481 GIGEDKQRSARLTAWFHTPQNYFVHEYEKHIKNLQSKVLTSPISQTTSKTEELRQSKGGT 540

Query: 541 LHVYTDNPQVEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEA 600
           L VYTDNPQVEEFL+RPEF ITSDPKEADIIWTSMQIDEDT+KATGITD+QY+NQFPFEA
Sbjct: 541 LRVYTDNPQVEEFLNRPEFTITSDPKEADIIWTSMQIDEDTRKATGITDKQYVNQFPFEA 600

Query: 601 CLVMKHHLAETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMAR 660
           CLVMKHHLAETI+KAHGC EWLQPTYNLETHLSQLIGDY VRKRDGLNNLWILKPWNMAR
Sbjct: 601 CLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKRDGLNNLWILKPWNMAR 660

Query: 661 TIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADS 720
           TIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADS
Sbjct: 661 TIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADS 720

Query: 721 FWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRV 780
           FWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIA+FV+EFEQEHNVKWLDIHSRV
Sbjct: 721 FWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIADFVREFEQEHNVKWLDIHSRV 780

Query: 781 RSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEH 840
            SMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLD+SFQPKLLEVTYCPDCTRACKYDVE+
Sbjct: 781 TSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVEN 840

Query: 841 VFEDGVIKGREFYNHVFGCLFLNETTHVTPL 868
           VF  GVI+GREFYN++FGCLFLNETTHVTPL
Sbjct: 841 VFGGGVIEGREFYNYIFGCLFLNETTHVTPL 871

BLAST of MS023133 vs. TAIR 10
Match: AT1G77550.1 (tubulin-tyrosine ligases;tubulin-tyrosine ligases )

HSP 1 Score: 1216.4 bits (3146), Expect = 0.0e+00
Identity = 578/862 (67.05%), Postives = 709/862 (82.25%), Query Frame = 0

Query: 6   RIGTYEDFVEVHGLLLAASRLPQSLHRQLFQKLTSETFDGGAHFQVETFEDGRCRRLVLS 65
           +I +++DFV+VHG+LLAAS LP  L+ +LF+KL  +TFDGG +FQ+E+ ED + R+L+L+
Sbjct: 3   KIESFDDFVKVHGILLAASGLPPKLYPRLFEKLAYDTFDGGDYFQIESCEDDQRRKLLLT 62

Query: 66  SDSMAKESHVFLVDHAWTFRLSDAYNQLKEVSGLAERMASLMCVDIDMNLDEEEGVHSND 125
           ++SM K+S VFLVDHAWTFRL DAY QLKE+ GLAERMASLM VDID++  EEE      
Sbjct: 63  AESMPKDSDVFLVDHAWTFRLPDAYKQLKEIPGLAERMASLMSVDIDVDAGEEEVAE--- 122

Query: 126 NEVKQSVFELVESEIRGVKDNGNGTVRWLELEDLQINDDTLLSLDLTTKFPDLLALSLTG 185
              + SV +++++EIR   D G  ++RWLELE L + DD+LLSL L +KF DL+ALSL G
Sbjct: 123 ---ELSVDQIIDNEIRYAADKGYDSLRWLELEGLGV-DDSLLSLHLPSKFQDLVALSLIG 182

Query: 186 NKLENVDVVAREVSKFKHLRALWLNNNPVAENCGEQLQQRVLEGFPNLEICNSRFTHNFS 245
           NK+E+ DVV +E+ KFK+L+ALWLN+NPV +    Q+   +L+G P+LEI NS FT N+ 
Sbjct: 183 NKIESADVVIQEIVKFKNLKALWLNDNPVLQKSERQMADEILQGCPSLEIYNSCFTPNYG 242

Query: 246 EWALGFCGDIYEKDNPGSIYPPDHSLQCLETLDLSNRCIHNLINKAFSPVELPSLSYLNL 305
            WALGFCGDI+ KDNPG +   D  L  + +LDLSNR IHNL+NKAFS  E+P LS+LN+
Sbjct: 243 LWALGFCGDIFGKDNPGCV-QQDQPLCNVTSLDLSNRSIHNLVNKAFSVHEMPLLSHLNI 302

Query: 306 RGNPLGKNSVDDLLRILKEFPCLTSLEVDIPGPLGEKALDIIESLPNLNTLNGIDVAKIL 365
           RGN L +NSV +LL +LK FP L+SLEVDIPGPLG  AL+I+ESL NL+ LNG+D AKIL
Sbjct: 303 RGNSLDQNSVGELLEVLKLFPSLSSLEVDIPGPLGINALEILESLSNLSLLNGVDTAKIL 362

Query: 366 HSGKHVIDSMLLPRFPEWTPEEPLPDRVINAMWLYLMTYRLADEEKIDETSVWYVMDELG 425
            +GKHV+DSML PR PE  P++ L DRV++AMWLY + YRLAD+EK+DETS+WYVMDELG
Sbjct: 363 ETGKHVVDSMLQPRIPELNPDDTLVDRVLDAMWLYALNYRLADDEKLDETSLWYVMDELG 422

Query: 426 SALRHSDQPNFKVAPFLFMPEGKLESAISFTILWPIHNVQKGDECTRDYLFGIGEEKQRS 485
           SALRHSD+PNFKVAPFLFMP GKLESA+S++++WPI + QKGDECTRD+L GIGE+KQRS
Sbjct: 423 SALRHSDEPNFKVAPFLFMPSGKLESAVSYSVMWPIKHSQKGDECTRDFLSGIGEDKQRS 482

Query: 486 ARLTAWFHTPQNYFMHEYEKHIRNLQSKVLASPISQTMVNTEGLQQSKGGTLHVYTDNPQ 545
           ARLTAWF TP+NYF+HE+EK+ + LQ+K   S  S   V +  +Q S G  L VYTD PQ
Sbjct: 483 ARLTAWFQTPENYFIHEFEKYQQKLQAKAFESKPSNPSV-SRSIQHSDGSPLLVYTDLPQ 542

Query: 546 VEEFLDRPEFVITSDPKEADIIWTSMQIDEDTKKATGITDEQYINQFPFEACLVMKHHLA 605
           VEEFL RPEFVIT++PK+ADI+WTS+Q+D++ KK  GITD+QYINQFPFEACLVMKHHLA
Sbjct: 543 VEEFLTRPEFVITNEPKDADILWTSVQVDDELKKEVGITDDQYINQFPFEACLVMKHHLA 602

Query: 606 ETIQKAHGCAEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDTTVTDN 665
           ETIQ  +G  +WLQPTYNLET LSQ IGDY VRKRD LNNLWILKPWNMARTIDT++TDN
Sbjct: 603 ETIQMGYGSPKWLQPTYNLETQLSQFIGDYCVRKRDQLNNLWILKPWNMARTIDTSITDN 662

Query: 666 LSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVRLANNP 725
           LSAIIR+METGPKICQKYIEHPALF G KFDLRY+VLVRS+ PLEI+L + FWVRL+NNP
Sbjct: 663 LSAIIRMMETGPKICQKYIEHPALFKGNKFDLRYVVLVRSIDPLEIYLIEIFWVRLSNNP 722

Query: 726 YSLEKQSLFEYETHFTVMNYRGRLNHKNIAEFVKEFEQEHNVKWLDIHSRVRSMIRSVFE 785
           YSLEK S FEYETHFTVMNY  +LNHK  AEFV+EFEQEHNVKW+DIH +V+ +IR+VFE
Sbjct: 723 YSLEKHSFFEYETHFTVMNYGRKLNHKPTAEFVREFEQEHNVKWMDIHEKVKQVIRAVFE 782

Query: 786 SAAVVHPEMHSPFSRAMYGLDVMLDASFQPKLLEVTYCPDCTRACKYDVEHVFEDGVIKG 845
           +AA+ HPEM SP SRAMYG+DVMLD+SF+PK+LEVTYCPDC RACKYD+E +   G++KG
Sbjct: 783 AAALAHPEMQSPKSRAMYGVDVMLDSSFEPKILEVTYCPDCMRACKYDMETIDGKGIVKG 842

Query: 846 REFYNHVFGCLFLNETTHVTPL 868
            +F+N+VFGCLFL+ET HVTPL
Sbjct: 843 GDFFNNVFGCLFLDETAHVTPL 855