MS023122 (gene) Bitter gourd (TR) v1

Overview


Name	MS023122
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA-directed RNA polymerase III subunit rpc4
Location	scaffold78: 305754 .. 307163 (-)
RNA-Seq Expression	MS023122
Synteny	MS023122

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCGCACCTACACAAGTTGCATTTGGTTCTGGAGGTACATCAGCAACCATAAGATCATATGGTATTCCAAAAGCTGGAAATAGACCCAGAAATGAAGGTTGGCCACTAAACTACTTAAATCTATTCTACTCAAGATGTAATTGCTTGATTTACTGCACATGAGATGCTTCCAATGCACTAAACTGAAAGGTGACTGTGCAGATGGAACTCTTCCGTCTAGTATAAGCAAGGAGTATGTAGAACCATGGGTCAGTAACATTCAAATCCTCAATCGTTTTTCCTTATTATTCCTATTTGCATCTGCCTTCTCCTTTAGTATGTCAATTTAGTAATTCAAGTATCATTTTACTGCAGGATTATTACAGTTATTATCCTGTAACTCTTCCTTTGAGGAGACCATATTCAGGAGATCCAGGTATCTCTATAAGCGCGTCTTATTTACAAAAACTAAAAGGAAAATACTGCAATGGACAACTGGATTATCCTTAAATGCACTTTATTTTCTGTGTGTGTGGTTCGCATACTTCATATGACTTCTGGAGTACAGTTTTCATGGTGAACCATGAAAAATGTACTCGCATCTTTTGATTCAATTGTCTTCTCTAATTTATGCATTACTTCAAAGATTCTCTTAACGAGGAAGAATTTGGAGAGGCTTCAGAAAACAGAACCTACGATGAAAGTATGACAACTCCAGCAATGGATCTTGGTCTATTGGTTAGTCATTAGATTTATGTGGATATCATAGCGTGAGCCTCTTATTTCCGATTTTACGCTTAATGATTGATTCGCAGGAGGAGAATCCAGAAACAAATGTCTTTTTTCTACAAATACCACCTATGGTGCCAGTGACAAAGCAATCTTCTACCGTAGAAGGCATGGTGGCAGCCAGCAGCTCAGAACAAAGTAAGGGTTCACGAACTCGTCAGAACTTCTGCTCTCTGAATGAGTTACCTTCTGGCTCCATGGGTAAATTGCTTGTATACAGGAGTGGTGCTGTCAAGTTGAAGCTAGGCGATATTGTATATGACGTGAGTACTTTTATGCCAAGTTCCTCGTTATCTACTTATTTATCAACAAGATAGATCTCCTGAAATGAAATATTAGAAACATGGAATCTCTTCCTCAGCTTTCTCGACTTCACTTCATTGATAAAACAGCAGGACGTGTTATTGCAAGTCCTTATCAATTGAGGGGATATTTTTGGACATAATCTTTTGGGATTTTGCGTTCCTTCTCACATCAGTTTTAAAATACGGGCAGGTTTCTTCTGGAATGGATTGTGGCTTTGCACAAGACGTTGCAGCTATCAATGTGGAAGGGAAGCATTGCTGCATTGTTGGGGAGCTTAGCAAGCGTGCCATTTTAACTCCAGATGTGGACTCGATGTTAAAGGATATCGAGTATTTA

mRNA sequence

GCCGCACCTACACAAGTTGCATTTGGTTCTGGAGGTACATCAGCAACCATAAGATCATATGGTATTCCAAAAGCTGGAAATAGACCCAGAAATGAAGATGGAACTCTTCCGTCTAGTATAAGCAAGGAGTATGTAGAACCATGGGATTATTACAGTTATTATCCTGTAACTCTTCCTTTGAGGAGACCATATTCAGGAGATCCAGATTCTCTTAACGAGGAAGAATTTGGAGAGGCTTCAGAAAACAGAACCTACGATGAAAGTATGACAACTCCAGCAATGGATCTTGGTCTATTGGAGGAGAATCCAGAAACAAATGTCTTTTTTCTACAAATACCACCTATGGTGCCAGTGACAAAGCAATCTTCTACCGTAGAAGGCATGGTGGCAGCCAGCAGCTCAGAACAAAGTAAGGGTTCACGAACTCGTCAGAACTTCTGCTCTCTGAATGAGTTACCTTCTGGCTCCATGGGTAAATTGCTTGTATACAGGAGTGGTGCTGTCAAGTTGAAGCTAGGCGATATTGTATATGACGTTTCTTCTGGAATGGATTGTGGCTTTGCACAAGACGTTGCAGCTATCAATGTGGAAGGGAAGCATTGCTGCATTGTTGGGGAGCTTAGCAAGCGTGCCATTTTAACTCCAGATGTGGACTCGATGTTAAAGGATATCGAGTATTTA

Coding sequence (CDS)

GCCGCACCTACACAAGTTGCATTTGGTTCTGGAGGTACATCAGCAACCATAAGATCATATGGTATTCCAAAAGCTGGAAATAGACCCAGAAATGAAGATGGAACTCTTCCGTCTAGTATAAGCAAGGAGTATGTAGAACCATGGGATTATTACAGTTATTATCCTGTAACTCTTCCTTTGAGGAGACCATATTCAGGAGATCCAGATTCTCTTAACGAGGAAGAATTTGGAGAGGCTTCAGAAAACAGAACCTACGATGAAAGTATGACAACTCCAGCAATGGATCTTGGTCTATTGGAGGAGAATCCAGAAACAAATGTCTTTTTTCTACAAATACCACCTATGGTGCCAGTGACAAAGCAATCTTCTACCGTAGAAGGCATGGTGGCAGCCAGCAGCTCAGAACAAAGTAAGGGTTCACGAACTCGTCAGAACTTCTGCTCTCTGAATGAGTTACCTTCTGGCTCCATGGGTAAATTGCTTGTATACAGGAGTGGTGCTGTCAAGTTGAAGCTAGGCGATATTGTATATGACGTTTCTTCTGGAATGGATTGTGGCTTTGCACAAGACGTTGCAGCTATCAATGTGGAAGGGAAGCATTGCTGCATTGTTGGGGAGCTTAGCAAGCGTGCCATTTTAACTCCAGATGTGGACTCGATGTTAAAGGATATCGAGTATTTA

Protein sequence

AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPLRRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTKQSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL

Homology

[Close]

BLAST of MS023122 vs. NCBI nr
Match: XP_022147557.1 (DNA-directed RNA polymerase III subunit rpc4 [Momordica charantia])

HSP 1 Score: 448.4 bits (1152), Expect = 3.8e-122
Identity = 226/227 (99.56%), Postives = 226/227 (99.56%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 60
           AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 126

Query: 61  RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 120
           RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK
Sbjct: 127 RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 186

Query: 121 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 180
           QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS
Sbjct: 187 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 246

Query: 181 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL 228
           SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIE L
Sbjct: 247 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEDL 293

[Close]

BLAST of MS023122 vs. NCBI nr
Match: XP_038878504.1 (uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878505.1 uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878507.1 uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878508.1 uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878509.1 uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878510.1 uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878511.1 uncharacterized protein LOC120070724 [Benincasa hispida])

HSP 1 Score: 393.3 bits (1009), Expect = 1.4e-105
Identity = 196/227 (86.34%), Postives = 211/227 (92.95%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 60
           AAPTQVAFGSGGTS+T+RSYG+ KAGNRPRNEDGTLPSS SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGTSSTLRSYGVSKAGNRPRNEDGTLPSSTSKEYVEPWDYYSYYPVTLPL 126

Query: 61  RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 120
           RRPYSG+PDSLNEEEFGEASEN  YDE+ TTPAMDLGLLEENPE +VFFLQ+PPMVP+ K
Sbjct: 127 RRPYSGNPDSLNEEEFGEASENFAYDENTTTPAMDLGLLEENPEMDVFFLQLPPMVPMVK 186

Query: 121 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 180
           QSSTVEG  AA+S EQSKGS+ RQ  C +NELPSGSMGKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSTVEGTGAANSLEQSKGSQPRQKACLMNELPSGSMGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 181 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL 228
           SGMDCGFAQDVAA+NVEGK CCIVGELSKRAILTPDVDS+LK+IE L
Sbjct: 247 SGMDCGFAQDVAAVNVEGKCCCIVGELSKRAILTPDVDSVLKNIEDL 293

[Close]

BLAST of MS023122 vs. NCBI nr
Match: XP_008450913.1 (PREDICTED: DNA-directed RNA polymerase III subunit RPC4 [Cucumis melo])

HSP 1 Score: 388.3 bits (996), Expect = 4.7e-104
Identity = 191/227 (84.14%), Postives = 212/227 (93.39%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 60
           AAPTQVAFGSGG+S+T+RSYG+ KAGNRPRNEDGTLP+S SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGSSSTLRSYGVSKAGNRPRNEDGTLPASTSKEYVEPWDYYSYYPVTLPL 126

Query: 61  RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 120
           RRPYSG+PDSLNEEEFGEASEN TYDE+ TT AM+LGLLEENPET+VFFLQ+PPMVP+ K
Sbjct: 127 RRPYSGNPDSLNEEEFGEASENFTYDENTTTAAMNLGLLEENPETDVFFLQLPPMVPMIK 186

Query: 121 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 180
           QSSTVE M + + SEQ+  S+TRQ  CS+NELPSGSMGKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSTVENMGSGNVSEQNSASQTRQKTCSMNELPSGSMGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 181 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL 228
           SGMDCGFAQ+VAAIN+EGKHCCIVGELSKRAILTPDVDSML++IE L
Sbjct: 247 SGMDCGFAQEVAAINMEGKHCCIVGELSKRAILTPDVDSMLRNIEDL 293

[Close]

BLAST of MS023122 vs. NCBI nr
Match: XP_004144123.1 (DNA-directed RNA polymerase III subunit RPC4 [Cucumis sativus] >XP_031736467.1 DNA-directed RNA polymerase III subunit RPC4 [Cucumis sativus] >XP_031736470.1 DNA-directed RNA polymerase III subunit RPC4 [Cucumis sativus] >KGN66283.1 hypothetical protein Csa_007538 [Cucumis sativus])

HSP 1 Score: 382.1 bits (980), Expect = 3.3e-102
Identity = 189/227 (83.26%), Postives = 210/227 (92.51%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 60
           AAPTQVAFGSGG+S+T+RSYG+ KAGNRPRNEDGTLP+S SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGSSSTLRSYGVSKAGNRPRNEDGTLPASTSKEYVEPWDYYSYYPVTLPL 126

Query: 61  RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 120
           RRPYSG+PDSLNEEEFGEASEN TYDE+ TT AM+LGLLEENPE +V FLQ+PPMVP+ K
Sbjct: 127 RRPYSGNPDSLNEEEFGEASENLTYDENTTTAAMNLGLLEENPEADVLFLQLPPMVPMIK 186

Query: 121 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 180
           QSS+VE M + +SSEQ+K S+ RQ  CS+NELPSGS+GKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSSVEDMGSGNSSEQNKASQPRQKTCSMNELPSGSIGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 181 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL 228
           SGMDCGFAQ+VAAINVEGK CCIVGELSKRAILTPDVDSMLK+IE L
Sbjct: 247 SGMDCGFAQEVAAINVEGKRCCIVGELSKRAILTPDVDSMLKNIEDL 293

[Close]

BLAST of MS023122 vs. NCBI nr
Match: KAG6588073.1 (DNA-directed RNA polymerase III subunit RPC4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 376.3 bits (965), Expect = 1.8e-100
Identity = 188/227 (82.82%), Postives = 209/227 (92.07%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 60
           +APTQVAFG+GGTS+++R+YG  KAGN+ RNEDGTLPS  SKEYVEPWDYYSYYPVTLPL
Sbjct: 55  SAPTQVAFGTGGTSSSLRTYGFSKAGNKLRNEDGTLPS--SKEYVEPWDYYSYYPVTLPL 114

Query: 61  RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 120
           RRPYSG+PDSLNE EFGEASE RTYDE+ TTPAMDLGLLE+NPETN+FFLQ+PPMVPVT+
Sbjct: 115 RRPYSGNPDSLNEYEFGEASEARTYDENTTTPAMDLGLLEDNPETNIFFLQLPPMVPVTE 174

Query: 121 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 180
           QSST EGM AA+S EQ+KGS+  Q  CSLNE PSGSMGKLLVYRSGAVKLKLG+IVYDVS
Sbjct: 175 QSSTAEGMEAANSLEQNKGSQPPQKTCSLNEFPSGSMGKLLVYRSGAVKLKLGNIVYDVS 234

Query: 181 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL 228
           SGMDCGFAQ++AAINVEGK CCIVGELSKRA+LTPDVDSMLK+IE L
Sbjct: 235 SGMDCGFAQELAAINVEGKRCCIVGELSKRAVLTPDVDSMLKNIEDL 279

[Close]

BLAST of MS023122 vs. ExPASy Swiss-Prot
Match: Q91WD1 (DNA-directed RNA polymerase III subunit RPC4 OS=Mus musculus OX=10090 GN=Polr3d PE=2 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 4.1e-10
Identity = 39/149 (26.17%), Postives = 75/149 (50.34%), Query Frame = 0


Query: 86  DESMTTPAMDLGLLEENPETNVFFLQIPPMV----------PVTKQSSTVEGMVAASSSE 145
           D S+     +L L+++     + FLQ+P  +          PV  +    +G +     E
Sbjct: 248 DVSVAELLRELSLMKDE---ELLFLQLPDTLPGQPPTQDIKPVKTEVQGEDGQMVVIKQE 307

Query: 146 QSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAIN 205
           + + +R  +N C+L +L  G +GKLL+ +SG V+L LG +  DV+ G  C F Q++ ++ 
Sbjct: 308 KDREARLAENACTLADLTEGQVGKLLIRKSGKVQLLLGKVTLDVTMGTTCSFLQELVSVG 367

Query: 206 VEGK---HCCIVGELSKRAILTPDVDSML 222
           +         ++G +  + + +PD +S+L
Sbjct: 368 LGDSRTGEMTVLGHVKHKLVCSPDFESLL 393

[Close]

BLAST of MS023122 vs. ExPASy Swiss-Prot
Match: Q5E9Z7 (DNA-directed RNA polymerase III subunit RPC4 OS=Bos taurus OX=9913 GN=POLR3D PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.3e-10
Identity = 38/149 (25.50%), Postives = 75/149 (50.34%), Query Frame = 0


Query: 86  DESMTTPAMDLGLLEENPETNVFFLQIPPMV----------PVTKQSSTVEGMVAASSSE 145
           D S+     +L L +E     + FLQ+P  +          P+  +  + +G +     E
Sbjct: 248 DVSVAELLRELSLTQEE---ELLFLQLPDSLPGQPPTQDIKPIKTEVQSEDGQMVVIKQE 307

Query: 146 QSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAIN 205
           + + +R  +N C+L +L  G +GKLL+ +SG V+L LG +  DV+ G  C F Q++ ++ 
Sbjct: 308 KDREARLAENTCTLADLTEGQVGKLLIRKSGKVQLLLGKVTLDVTMGTTCSFLQELVSVG 367

Query: 206 VEGKH---CCIVGELSKRAILTPDVDSML 222
           +         ++G +  + + +P+ +S+L
Sbjct: 368 LGDSRTGDMTVLGHIKHKLVCSPNFESLL 393

[Close]

BLAST of MS023122 vs. ExPASy Swiss-Prot
Match: P05423 (DNA-directed RNA polymerase III subunit RPC4 OS=Homo sapiens OX=9606 GN=POLR3D PE=1 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 6.9e-10
Identity = 35/131 (26.72%), Postives = 67/131 (51.15%), Query Frame = 0


Query: 104 ETNVFFLQIPPMV----------PVTKQSSTVEGMVAASSSEQSKGSRTRQNFCSLNELP 163
           E  + FLQ+P  +          P+  +    +G V     E+ + ++  +N C+L +L 
Sbjct: 263 EEELLFLQLPDTLPGQPPTQDIKPIKTEVQGEDGQVVLIKQEKDREAKLAENACTLADLT 322

Query: 164 SGSMGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINVEGK---HCCIVGELSKR 222
            G +GKLL+ +SG V+L LG +  DV+ G  C F Q++ ++ +         ++G +  +
Sbjct: 323 EGQVGKLLIRKSGRVQLLLGKVTLDVTMGTACSFLQELVSVGLGDSRTGEMTVLGHVKHK 382

[Close]

BLAST of MS023122 vs. ExPASy Swiss-Prot
Match: O74857 (DNA-directed RNA polymerase III subunit rpc4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpc53 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.0e-05
Identity = 39/145 (26.90%), Postives = 63/145 (43.45%), Query Frame = 0


Query: 84  TYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPV-----TKQSSTVEGMVAASSSEQSK 143
           T  E M     D  +L + P+    F+    + PV      K+    E        +  K
Sbjct: 185 TEQEGMEDHFKDQMVLMQFPDKLPRFMGDADVDPVWPSSEVKEEEAGEKTDVEEKKDPDK 244

Query: 144 GSRTRQNFCSLNEL-------PSGSMGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDV 203
             R   N   L  L       P+G +G L V++SG   L++G + + V SG DC F Q++
Sbjct: 245 HGRENGNIDDLTSLPYPAFHPPAGQIGVLRVHQSGKTTLEMGGVNFVVQSGSDCLFLQEI 304

Query: 204 AAINVEGKHCCIVGELSKRAILTPD 217
           A ++ + K    +G   +R I++PD
Sbjct: 305 AVVDYDSKRIWNLGSFERRMIVSPD 329

[Close]

BLAST of MS023122 vs. ExPASy Swiss-Prot
Match: Q8T1V7 (DNA-directed RNA polymerase III subunit rpc4 OS=Dictyostelium discoideum OX=44689 GN=polr3d PE=3 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 3.9e-05
Identity = 25/78 (32.05%), Postives = 41/78 (52.56%), Query Frame = 0


Query: 148 SLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINVEG-----KHCC 207
           S+ E+PSG +G L +Y+SG   LK+G + YD+SSG    F ++V     +G       C 
Sbjct: 350 SIKEMPSGKLGTLKIYKSGKSILKIGTVEYDISSGDKLKFLEEVQCFQTKGGPDNSPTCY 409

Query: 208 IVGELSKRAILTPDVDSM 221
            +G  S+  +  P +  +
Sbjct: 410 TLGIPSQHLVAAPIISQI 427

[Close]

BLAST of MS023122 vs. ExPASy TrEMBL
Match: A0A6J1D2P1 (DNA-directed RNA polymerase III subunit rpc4 OS=Momordica charantia OX=3673 GN=LOC111016454 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.8e-122
Identity = 226/227 (99.56%), Postives = 226/227 (99.56%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 60
           AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 126

Query: 61  RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 120
           RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK
Sbjct: 127 RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 186

Query: 121 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 180
           QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS
Sbjct: 187 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 246

Query: 181 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL 228
           SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIE L
Sbjct: 247 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEDL 293

[Close]

BLAST of MS023122 vs. ExPASy TrEMBL
Match: A0A1S3BPR1 (DNA-directed RNA polymerase III subunit RPC4 OS=Cucumis melo OX=3656 GN=LOC103492360 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.3e-104
Identity = 191/227 (84.14%), Postives = 212/227 (93.39%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 60
           AAPTQVAFGSGG+S+T+RSYG+ KAGNRPRNEDGTLP+S SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGSSSTLRSYGVSKAGNRPRNEDGTLPASTSKEYVEPWDYYSYYPVTLPL 126

Query: 61  RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 120
           RRPYSG+PDSLNEEEFGEASEN TYDE+ TT AM+LGLLEENPET+VFFLQ+PPMVP+ K
Sbjct: 127 RRPYSGNPDSLNEEEFGEASENFTYDENTTTAAMNLGLLEENPETDVFFLQLPPMVPMIK 186

Query: 121 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 180
           QSSTVE M + + SEQ+  S+TRQ  CS+NELPSGSMGKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSTVENMGSGNVSEQNSASQTRQKTCSMNELPSGSMGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 181 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL 228
           SGMDCGFAQ+VAAIN+EGKHCCIVGELSKRAILTPDVDSML++IE L
Sbjct: 247 SGMDCGFAQEVAAINMEGKHCCIVGELSKRAILTPDVDSMLRNIEDL 293

[Close]

BLAST of MS023122 vs. ExPASy TrEMBL
Match: A0A0A0LWX7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G595870 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.6e-102
Identity = 189/227 (83.26%), Postives = 210/227 (92.51%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 60
           AAPTQVAFGSGG+S+T+RSYG+ KAGNRPRNEDGTLP+S SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGSSSTLRSYGVSKAGNRPRNEDGTLPASTSKEYVEPWDYYSYYPVTLPL 126

Query: 61  RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 120
           RRPYSG+PDSLNEEEFGEASEN TYDE+ TT AM+LGLLEENPE +V FLQ+PPMVP+ K
Sbjct: 127 RRPYSGNPDSLNEEEFGEASENLTYDENTTTAAMNLGLLEENPEADVLFLQLPPMVPMIK 186

Query: 121 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 180
           QSS+VE M + +SSEQ+K S+ RQ  CS+NELPSGS+GKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSSVEDMGSGNSSEQNKASQPRQKTCSMNELPSGSIGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 181 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL 228
           SGMDCGFAQ+VAAINVEGK CCIVGELSKRAILTPDVDSMLK+IE L
Sbjct: 247 SGMDCGFAQEVAAINVEGKRCCIVGELSKRAILTPDVDSMLKNIEDL 293

[Close]

BLAST of MS023122 vs. ExPASy TrEMBL
Match: A0A6J1HTZ8 (uncharacterized protein LOC111467450 OS=Cucurbita maxima OX=3661 GN=LOC111467450 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 7.5e-100
Identity = 186/227 (81.94%), Postives = 208/227 (91.63%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 60
           AAPTQVAFG+GGTS+++R++G  KAGN+ RNEDGTLP   S+EYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGTGGTSSSLRTFGFSKAGNKLRNEDGTLP---SREYVEPWDYYSYYPVTLPL 126

Query: 61  RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 120
           RRPYSG+PDSLNE EFGEASE+RTYDE+ TTPAMDLGLLE+NPETN+FFLQ+PPMVPVT+
Sbjct: 127 RRPYSGNPDSLNEYEFGEASESRTYDENTTTPAMDLGLLEDNPETNIFFLQLPPMVPVTE 186

Query: 121 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 180
           QSST EGM AA+S EQ+K S+  Q  CSLNE PSGSMGKLLVYRSGAVKLKLGDIVYDVS
Sbjct: 187 QSSTAEGMEAANSLEQNKDSQPPQKTCSLNEFPSGSMGKLLVYRSGAVKLKLGDIVYDVS 246

Query: 181 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL 228
           SGMDCGFAQ++AAINVEGK CCIVGELSKRA+LTPDVDSMLK+IE L
Sbjct: 247 SGMDCGFAQELAAINVEGKRCCIVGELSKRAVLTPDVDSMLKNIEDL 290

[Close]

BLAST of MS023122 vs. ExPASy TrEMBL
Match: A0A6J1F0U0 (DNA-directed RNA polymerase III subunit RPC4-like OS=Cucurbita moschata OX=3662 GN=LOC111441105 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 7.5e-100
Identity = 188/227 (82.82%), Postives = 208/227 (91.63%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 60
           AAPTQVAFG+GGTS+++R+YG  KAGN+ RNEDGTLPS  SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGTGGTSSSLRTYGSSKAGNKLRNEDGTLPS--SKEYVEPWDYYSYYPVTLPL 126

Query: 61  RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 120
           RRPYSG+PDSLNE EFGEASE RTYDE+ TTPAMDLGLLE+NPETN+FFLQ+PPMVPVT+
Sbjct: 127 RRPYSGNPDSLNEYEFGEASEARTYDENTTTPAMDLGLLEDNPETNIFFLQLPPMVPVTE 186

Query: 121 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 180
           QSST EGM AA+S EQ+K S+  Q  CSLNE PSGSMGKLLVYRSGAVKLKLG+IVYDVS
Sbjct: 187 QSSTAEGMEAANSLEQNKVSQPPQKTCSLNEFPSGSMGKLLVYRSGAVKLKLGNIVYDVS 246

Query: 181 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEYL 228
           SGMDCGFAQ++AAINVEGK CCIVGELSKRA+LTPDVDSMLK+IE L
Sbjct: 247 SGMDCGFAQELAAINVEGKRCCIVGELSKRAVLTPDVDSMLKNIEDL 291

[Close]

BLAST of MS023122 vs. TAIR 10
Match: AT5G09380.1 (RNA polymerase III RPC4 )

HSP 1 Score: 175.6 bits (444), Expect = 4.4e-44
Identity = 100/222 (45.05%), Postives = 146/222 (65.77%), Query Frame = 0


Query: 4   TQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPLRRP 63
           +QVA+  G  ++T  +    K  NR     G+  S+   EY EPWDYYSYYP+TLP+RRP
Sbjct: 64  SQVAWLGGVVNSTRSN----KYLNRSNGAYGS-TSTQEIEYKEPWDYYSYYPITLPMRRP 123

Query: 64  YSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTKQSS 123
           Y+GDP+ L+ EEF +A  +  +++S+ T A +LGL+E++ E  +FF+++P +   +  + 
Sbjct: 124 YAGDPEVLDVEEFMQAGGH--HEDSLNT-AANLGLMEDSGEQKMFFMRLPSVPLASTPTE 183

Query: 124 TVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVSSGM 183
            +E      +    KG    +    L  LP G MGKLLVY+SGAVK+KLG+++YDVS G+
Sbjct: 184 NLE------TRPNIKGP-VEKKTVDLKALPEGYMGKLLVYKSGAVKMKLGEVLYDVSPGL 243

Query: 184 DCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIE 226
              FAQDV  +N E K+CC+VG++ K A+LTPD+DS+LKDIE
Sbjct: 244 KSEFAQDVMVVNTEQKNCCLVGDVYKHAVLTPDIDSILKDIE 270

[Close]

BLAST of MS023122 vs. TAIR 10
Match: AT4G25180.1 (RNA polymerase III RPC4 )

HSP 1 Score: 154.1 bits (388), Expect = 1.4e-37
Identity = 96/248 (38.71%), Postives = 138/248 (55.65%), Query Frame = 0


Query: 1   AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNE-DGTLPSSI------------------- 60
           A+  +VAF    +   IRS+G+PK  ++P ++ + + P+SI                   
Sbjct: 61  ASSPEVAFQPSLSPLAIRSFGVPKEDDKPNSDVNPSSPASILPAVSSVTAAQEDGEEVHN 120

Query: 61  -----SKEYVEPWDY-YSYYPVTLPLRRPYSGDPDSLNEEEFGEASENRTYDESMTTPAM 120
                  +YVEPWDY  SYYP  LPLR+P SGD + L++EEFGE ++NR YDE+    A 
Sbjct: 121 FVTRTGDDYVEPWDYRNSYYPTVLPLRKPNSGDIELLDQEEFGEVAKNRDYDENTINSAE 180

Query: 121 DLGLLE-ENPETNVFFLQIPPMVPVTKQSSTVEGMVAASSSEQSKGSRTRQNFCSLNELP 180
           +LGL   ++ +  +F  +IP  +PV KQ  T       S  E S G            LP
Sbjct: 181 ELGLTSVQHSKKQMFIFKIPDCLPVVKQ--TTGATTKRSVREYSSGISN-----PFEGLP 240

Query: 181 SGSMGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINVEGKHCCIVGELSKRAIL 222
            G MGK+LVY+SGAVKLK+GD ++DVS G       DV AI+++G++C  +G  +K   +
Sbjct: 241 EGFMGKMLVYKSGAVKLKVGDALFDVSPGPGTKIPNDVVAIDIKGRNCSRIGSSAKFVTV 300

The following BLAST results are available for this feature:

BLAST of MS023122 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147557.1	3.8e-122	99.56	DNA-directed RNA polymerase III subunit rpc4 [Momordica charantia]	[more]
XP_038878504.1	1.4e-105	86.34	uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878505.1 unchara...	[more]
XP_008450913.1	4.7e-104	84.14	PREDICTED: DNA-directed RNA polymerase III subunit RPC4 [Cucumis melo]	[more]
XP_004144123.1	3.3e-102	83.26	DNA-directed RNA polymerase III subunit RPC4 [Cucumis sativus] >XP_031736467.1 D...	[more]
KAG6588073.1	1.8e-100	82.82	DNA-directed RNA polymerase III subunit RPC4, partial [Cucurbita argyrosperma su...	[more]

BLAST of MS023122 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q91WD1	4.1e-10	26.17	DNA-directed RNA polymerase III subunit RPC4 OS=Mus musculus OX=10090 GN=Polr3d ...	[more]
Q5E9Z7	5.3e-10	25.50	DNA-directed RNA polymerase III subunit RPC4 OS=Bos taurus OX=9913 GN=POLR3D PE=...	[more]
P05423	6.9e-10	26.72	DNA-directed RNA polymerase III subunit RPC4 OS=Homo sapiens OX=9606 GN=POLR3D P...	[more]
O74857	1.0e-05	26.90	DNA-directed RNA polymerase III subunit rpc4 OS=Schizosaccharomyces pombe (strai...	[more]
Q8T1V7	3.9e-05	32.05	DNA-directed RNA polymerase III subunit rpc4 OS=Dictyostelium discoideum OX=4468...	[more]

BLAST of MS023122 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2P1	1.8e-122	99.56	DNA-directed RNA polymerase III subunit rpc4 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A1S3BPR1	2.3e-104	84.14	DNA-directed RNA polymerase III subunit RPC4 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A0A0LWX7	1.6e-102	83.26	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G595870 PE=4 SV=1	[more]
A0A6J1HTZ8	7.5e-100	81.94	uncharacterized protein LOC111467450 OS=Cucurbita maxima OX=3661 GN=LOC111467450...	[more]
A0A6J1F0U0	7.5e-100	82.82	DNA-directed RNA polymerase III subunit RPC4-like OS=Cucurbita moschata OX=3662 ...	[more]

BLAST of MS023122 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09380.1	4.4e-44	45.05	RNA polymerase III RPC4	[more]
AT4G25180.1	1.4e-37	38.71	RNA polymerase III RPC4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007811	DNA-directed RNA polymerase III subunit RPC4	PFAM	PF05132	RNA_pol_Rpc4	coord: 115..217 e-value: 1.0E-22 score: 81.1
IPR007811	DNA-directed RNA polymerase III subunit RPC4	PANTHER	PTHR13408	DNA-DIRECTED RNA POLYMERASE III	coord: 38..224
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 65..86
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..17
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..39
None	No IPR available	PANTHER	PTHR13408:SF6	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4-LIKE ISOFORM X1	coord: 38..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS023122.1	MS023122.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006383	transcription by RNA polymerase III
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding