MS023121 (gene) Bitter gourd (TR) v1

Overview
NameMS023121
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncytochrome P450 CYP72A219-like
Locationscaffold78: 302145 .. 304693 (+)
RNA-Seq ExpressionMS023121
SyntenyMS023121
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGGTTTTGTGAAGGAAAAAAAAAATATGGAGTTTTTCGTTTTCTATGCATTGTCACTCTGTTTGGTTGTTTATGGAGTGGCAAAAGCTTTCCATTCCATTTGGTGGAGACCCAAATTGGTGGAGAGACAGTTGAAAAGGCAAGGAATTAGAGGGAGTGCCTACAAGCCTTTGGTTGGGGATGCCAAAGAGTATGTGAGGATGATCACTGAAGCCTGGGCCAACCCCATGAGTCTAGACCATCAGATTGTTCAACGGGTAGATCCGTTCACGGTGGTGAATGCCCAAAAATATGGTGCATCGTGTTCTATTGTGTTGTTATCTTGTTTGATATTGTTCTCGAGTCTTATTTTTGTGATTTGTGTGTAGGGAAAGTGTCGGTGTGCTGGTTCGGGACGAGTCCAAGGTTGACTGTGAAGGACCCGGAAATGATGAAAGAAATACTATCAAACAAGGTGGGTAGCTTTGAGAAGCCGCCATTGAGCCCAGTTATACTGAAGTTGACAAAGGGACTGACTACTCTCAAAGGGGAGGAGTGGGCTAAACATAGGAGGATTATCAATCCTGCTTTCCATCTAGAAAGGCTCAAGGTTTGAGTTTAACTCTACTCAGTTTTACTTGAAATAAGAGTTATGATGTAAGCTCAAGACTTGTTTGTGAAATTGTTTCGAAAACAGGAGAAAAAGCGAGAAAGAAATAGATGTAGAAATATAAACCATTTCCAAAATAAAGGGGACTGAATAGGAACAAGAGGGGTCAACTGAAGAAGATCCTTCCCCTTCCCATCTTTCAAAAGAAAAGATTCAGCCAAAATCAATAAAACGGAAGAAATCCAAGCGAACGGAAAAGAAGAAACAAAGGATGAATTCCACTTTAAGCACTATATATTTATTTGTGTTCATGGTTTGTTCTTTGAAGTTTTTTATTTTTTGTCTAATTCTGGTTAAATTTTAAATATCATTCTGAGCCTAAACTATGGTGTTTAAATTTTAAGACTGTTGTACGTTTGGGTGTTAAAGGGAATGATGCCAGCATTTAGTATAAGTTGTGGCAACATGATAAAGCAGTGGAAGCAGATGGGTACAGATCAAGACAGAGTAGAAGTGGATGTGTGGCCTGAACTTCAAAGACTAACGGCAGATGTTATATCTCGGGCTGCTTTTGGCTCCAACTTTGAAGAAGGGAAGAAGATTTTTGAACATTTGAAACGGCTCACTCTTCTTACTCTAGAAGCCATGCAAACCATGTATCTTCCTGGTTTCAGGTCCCACTTCTCTGTTTCATAAATTTCGTTTGTCTTCCCACCTATTCGTTTCCAAATGAATATTGTTTTCACTCGACTTAGATTGTTAGTCCTTAAACTTTCAGAATGGTGTCTAACAGGTCATTGATTTATTAGACCGTTTTTAAATTTTAAAGACTTATGGACACAAAAGAGCATAAATCTAAAACTAAAATTATTCTGAAATCAAACCTAGAGATTGAATAGTGTCTGTGTATTGGCAGATTTGTTCCAACAGTCAAGAACAGGAAGAGAGATAAGCTGAACAAGGAGATCAAAACTATGATAGGGAATTTAATTATAAGAAAAGAGGATTCCATGAGAACACAGGAAGACAATGTTGAAGACTTGTTGTGCCTGCTATTGCAGTCAAAGAAGAAAGAAAATCAACATGGAGGTCATGATGGGCTGACAATTGATGAAGTGATGGAGGAGTGCAAGCAATTCTACCTAGCAGGGCAGGAGACAACAGCAAGCTGGCTTACATGGACAGTCATAGTTTTGGCCATGCACTCAGAATGGCAAGAAAAGGCTAGGCAGGAAGTCTTGCAAGTTTGTGGGAAGAAAGAACCAAATTTTGAATCTTTGAGCCACCTCAAAATTGTGAGTTTTCCTTACACATTCAGCAATCTTCTATATATCTATTGGTGTAAGTTCTTTCATGACATTCAATCACATACAAATAGCAAAATGAAGAATTGAGAACAGTTGAAGTCATTTTCTGGTTGGTCAATATCCAAATCCTTTCTAAGTGGATATATTATTGGCAACCCTTATTCATCTATATATTTTGAAAACATCTATAAAGAAAAACAATATTGATTTACTAAACAATGTTCTGCAGGTTACTATGATACTTTATGAGGTCCTGAGGTTGTACCCACCAGTTATTGGTCAATATCAACATACTTATGCAGAAACTAAGATAGGTGAACTTTTAATACCAGCTGGGGTTGATGTTACCTTACCTACACTGCTCATTCACCATGATCCCGAGTACTGGGGAGACGATGCAGACCAGTTCAAACCAGAGAGATTTTCTGCAGGAGTTTCAAAGGCAAGCAAAGATCAGTTGGCATTCTTTCCCTTTGGCTGGGGACCAAGGACCTGCATTGGACAAAACTTTGCTATGTTAGAAGCTAAGGTTGCATTGGCTATGATTCTACAGAATTTCTCTTTTGAGCTTTCTCCCTCTTATGCACATGCTCCCTATACTGTCATGACTCTTCAACCACAACATGGGGCACAGCTGACCCTTCACCAACTA

mRNA sequence

TTTGGTTTTGTGAAGGAAAAAAAAAATATGGAGTTTTTCGTTTTCTATGCATTGTCACTCTGTTTGGTTGTTTATGGAGTGGCAAAAGCTTTCCATTCCATTTGGTGGAGACCCAAATTGGTGGAGAGACAGTTGAAAAGGCAAGGAATTAGAGGGAGTGCCTACAAGCCTTTGGTTGGGGATGCCAAAGAGTATGTGAGGATGATCACTGAAGCCTGGGCCAACCCCATGAGTCTAGACCATCAGATTGTTCAACGGGTAGATCCGTTCACGGTGGTGAATGCCCAAAAATATGGGAAAGTGTCGGTGTGCTGGTTCGGGACGAGTCCAAGGTTGACTGTGAAGGACCCGGAAATGATGAAAGAAATACTATCAAACAAGGTGGGTAGCTTTGAGAAGCCGCCATTGAGCCCAGTTATACTGAAGTTGACAAAGGGACTGACTACTCTCAAAGGGGAGGAGTGGGCTAAACATAGGAGGATTATCAATCCTGCTTTCCATCTAGAAAGGCTCAAGGGAATGATGCCAGCATTTAGTATAAGTTGTGGCAACATGATAAAGCAGTGGAAGCAGATGGGTACAGATCAAGACAGAGTAGAAGTGGATGTGTGGCCTGAACTTCAAAGACTAACGGCAGATGTTATATCTCGGGCTGCTTTTGGCTCCAACTTTGAAGAAGGGAAGAAGATTTTTGAACATTTGAAACGGCTCACTCTTCTTACTCTAGAAGCCATGCAAACCATGTATCTTCCTGGTTTCAGATTTGTTCCAACAGTCAAGAACAGGAAGAGAGATAAGCTGAACAAGGAGATCAAAACTATGATAGGGAATTTAATTATAAGAAAAGAGGATTCCATGAGAACACAGGAAGACAATGTTGAAGACTTGTTGTGCCTGCTATTGCAGTCAAAGAAGAAAGAAAATCAACATGGAGGTCATGATGGGCTGACAATTGATGAAGTGATGGAGGAGTGCAAGCAATTCTACCTAGCAGGGCAGGAGACAACAGCAAGCTGGCTTACATGGACAGTCATAGTTTTGGCCATGCACTCAGAATGGCAAGAAAAGGCTAGGCAGGAAGTCTTGCAAGTTTGTGGGAAGAAAGAACCAAATTTTGAATCTTTGAGCCACCTCAAAATTGTTACTATGATACTTTATGAGGTCCTGAGGTTGTACCCACCAGTTATTGGTCAATATCAACATACTTATGCAGAAACTAAGATAGGTGAACTTTTAATACCAGCTGGGGTTGATGTTACCTTACCTACACTGCTCATTCACCATGATCCCGAGTACTGGGGAGACGATGCAGACCAGTTCAAACCAGAGAGATTTTCTGCAGGAGTTTCAAAGGCAAGCAAAGATCAGTTGGCATTCTTTCCCTTTGGCTGGGGACCAAGGACCTGCATTGGACAAAACTTTGCTATGTTAGAAGCTAAGGTTGCATTGGCTATGATTCTACAGAATTTCTCTTTTGAGCTTTCTCCCTCTTATGCACATGCTCCCTATACTGTCATGACTCTTCAACCACAACATGGGGCACAGCTGACCCTTCACCAACTA

Coding sequence (CDS)

TTTGGTTTTGTGAAGGAAAAAAAAAATATGGAGTTTTTCGTTTTCTATGCATTGTCACTCTGTTTGGTTGTTTATGGAGTGGCAAAAGCTTTCCATTCCATTTGGTGGAGACCCAAATTGGTGGAGAGACAGTTGAAAAGGCAAGGAATTAGAGGGAGTGCCTACAAGCCTTTGGTTGGGGATGCCAAAGAGTATGTGAGGATGATCACTGAAGCCTGGGCCAACCCCATGAGTCTAGACCATCAGATTGTTCAACGGGTAGATCCGTTCACGGTGGTGAATGCCCAAAAATATGGGAAAGTGTCGGTGTGCTGGTTCGGGACGAGTCCAAGGTTGACTGTGAAGGACCCGGAAATGATGAAAGAAATACTATCAAACAAGGTGGGTAGCTTTGAGAAGCCGCCATTGAGCCCAGTTATACTGAAGTTGACAAAGGGACTGACTACTCTCAAAGGGGAGGAGTGGGCTAAACATAGGAGGATTATCAATCCTGCTTTCCATCTAGAAAGGCTCAAGGGAATGATGCCAGCATTTAGTATAAGTTGTGGCAACATGATAAAGCAGTGGAAGCAGATGGGTACAGATCAAGACAGAGTAGAAGTGGATGTGTGGCCTGAACTTCAAAGACTAACGGCAGATGTTATATCTCGGGCTGCTTTTGGCTCCAACTTTGAAGAAGGGAAGAAGATTTTTGAACATTTGAAACGGCTCACTCTTCTTACTCTAGAAGCCATGCAAACCATGTATCTTCCTGGTTTCAGATTTGTTCCAACAGTCAAGAACAGGAAGAGAGATAAGCTGAACAAGGAGATCAAAACTATGATAGGGAATTTAATTATAAGAAAAGAGGATTCCATGAGAACACAGGAAGACAATGTTGAAGACTTGTTGTGCCTGCTATTGCAGTCAAAGAAGAAAGAAAATCAACATGGAGGTCATGATGGGCTGACAATTGATGAAGTGATGGAGGAGTGCAAGCAATTCTACCTAGCAGGGCAGGAGACAACAGCAAGCTGGCTTACATGGACAGTCATAGTTTTGGCCATGCACTCAGAATGGCAAGAAAAGGCTAGGCAGGAAGTCTTGCAAGTTTGTGGGAAGAAAGAACCAAATTTTGAATCTTTGAGCCACCTCAAAATTGTTACTATGATACTTTATGAGGTCCTGAGGTTGTACCCACCAGTTATTGGTCAATATCAACATACTTATGCAGAAACTAAGATAGGTGAACTTTTAATACCAGCTGGGGTTGATGTTACCTTACCTACACTGCTCATTCACCATGATCCCGAGTACTGGGGAGACGATGCAGACCAGTTCAAACCAGAGAGATTTTCTGCAGGAGTTTCAAAGGCAAGCAAAGATCAGTTGGCATTCTTTCCCTTTGGCTGGGGACCAAGGACCTGCATTGGACAAAACTTTGCTATGTTAGAAGCTAAGGTTGCATTGGCTATGATTCTACAGAATTTCTCTTTTGAGCTTTCTCCCTCTTATGCACATGCTCCCTATACTGTCATGACTCTTCAACCACAACATGGGGCACAGCTGACCCTTCACCAACTA

Protein sequence

FGFVKEKKNMEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMITEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVGSFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQWKQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMYLPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQHGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKEPNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHDPEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQNFSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL
Homology
BLAST of MS023121 vs. NCBI nr
Match: XP_022147674.1 (cytochrome P450 CYP72A219-like [Momordica charantia])

HSP 1 Score: 1044.6 bits (2700), Expect = 2.7e-301
Identity = 511/512 (99.80%), Postives = 511/512 (99.80%), Query Frame = 0

Query: 10  MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMI 69
           MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKP VGDAKEYVRMI
Sbjct: 1   MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPWVGDAKEYVRMI 60

Query: 70  TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG 129
           TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG
Sbjct: 61  TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG 120

Query: 130 SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW 189
           SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW
Sbjct: 121 SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW 180

Query: 190 KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY 249
           KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY
Sbjct: 181 KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY 240

Query: 250 LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ 309
           LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ
Sbjct: 241 LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ 300

Query: 310 HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE 369
           HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE
Sbjct: 301 HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE 360

Query: 370 PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD 429
           PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD
Sbjct: 361 PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD 420

Query: 430 PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 489
           PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN
Sbjct: 421 PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 480

Query: 490 FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL
Sbjct: 481 FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 512

BLAST of MS023121 vs. NCBI nr
Match: KAA0055790.1 (cytochrome P450 CYP72A219-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 926.8 bits (2394), Expect = 8.3e-266
Identity = 444/512 (86.72%), Postives = 485/512 (94.73%), Query Frame = 0

Query: 10  MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMI 69
           ME+ VFY+L+LCLVV+GVAKAFHSIWW+PKLVER LKRQGIRG+AYKPLVGDAKEYVRMI
Sbjct: 1   MEYSVFYSLALCLVVFGVAKAFHSIWWKPKLVERHLKRQGIRGNAYKPLVGDAKEYVRMI 60

Query: 70  TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG 129
           TEAW+ PM+LDHQIVQRVDPFT VNA+KYGK+S+CWFGTSPRL VKDPEMMKE+L NKVG
Sbjct: 61  TEAWSTPMNLDHQIVQRVDPFTAVNAKKYGKISMCWFGTSPRLIVKDPEMMKEVLMNKVG 120

Query: 130 SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW 189
           SFEKPPLSP+ILKLT+GLTTLKG+EWAKH+RIINPAFHLERLKGMMPAF+ISC NMI+ W
Sbjct: 121 SFEKPPLSPLILKLTRGLTTLKGKEWAKHKRIINPAFHLERLKGMMPAFNISCSNMIEDW 180

Query: 190 KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY 249
           K+   +QD VEVDVWPELQRLT DVISRAAFGSNFEEGKKIFEHLK+LTLLTLEAMQT+Y
Sbjct: 181 KEKAANQDSVEVDVWPELQRLTGDVISRAAFGSNFEEGKKIFEHLKQLTLLTLEAMQTLY 240

Query: 250 LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ 309
           LPGFRF+PT KNRKR+KLNKEIKTMIG+LI+RKE+SM  QEDNV+DLL LLLQSKKKENQ
Sbjct: 241 LPGFRFIPTAKNRKREKLNKEIKTMIGSLILRKENSMSNQEDNVDDLLSLLLQSKKKENQ 300

Query: 310 HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE 369
             G D LTI+EV+EECKQFYLAGQETTAS L WTVIVLAMHS+WQEKARQEVLQ+CGK E
Sbjct: 301 QEG-DELTIEEVVEECKQFYLAGQETTASLLIWTVIVLAMHSDWQEKARQEVLQICGKNE 360

Query: 370 PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD 429
           P+FESL+H KIVTMILYEVLRLYPPVIGQYQHTYAE KIGE+LIPAG+DVTLPTLLIHHD
Sbjct: 361 PSFESLNHFKIVTMILYEVLRLYPPVIGQYQHTYAEVKIGEVLIPAGIDVTLPTLLIHHD 420

Query: 430 PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 489
           PEYWG+DA+QFKPERF+AGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN
Sbjct: 421 PEYWGEDAEQFKPERFAAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 480

Query: 490 FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           FSF+LSPSYAHAP+TVMTLQPQHGAQL LHQL
Sbjct: 481 FSFQLSPSYAHAPHTVMTLQPQHGAQLILHQL 511

BLAST of MS023121 vs. NCBI nr
Match: XP_008450912.1 (PREDICTED: cytochrome P450 CYP72A219-like isoform X2 [Cucumis melo])

HSP 1 Score: 924.9 bits (2389), Expect = 3.2e-265
Identity = 444/512 (86.72%), Postives = 484/512 (94.53%), Query Frame = 0

Query: 10  MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMI 69
           ME+ VFY+L+LCLVV+GVAKAFHSIWW+PKLVER LKRQGIRG+AYKPLVGDAKEYVRMI
Sbjct: 1   MEYSVFYSLALCLVVFGVAKAFHSIWWKPKLVERHLKRQGIRGNAYKPLVGDAKEYVRMI 60

Query: 70  TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG 129
           TEAW+ PM+LDHQIVQRVDPFT VNA+KYGK+S+CWFGTSPRL VKDPEMMKE+L NKVG
Sbjct: 61  TEAWSTPMNLDHQIVQRVDPFTAVNAKKYGKISMCWFGTSPRLIVKDPEMMKEVLMNKVG 120

Query: 130 SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW 189
           SFEKPPLSP+ILKLT+GLTTLKG+EWAKH+RIINPAFHLERLK MMPAF+ISC NMI+ W
Sbjct: 121 SFEKPPLSPLILKLTRGLTTLKGKEWAKHKRIINPAFHLERLKRMMPAFNISCSNMIEDW 180

Query: 190 KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY 249
           K+   +QD VEVDVWPELQRLT DVISRAAFGSNFEEGKKIFEHLK+LTLLTLEAMQTMY
Sbjct: 181 KEKAANQDSVEVDVWPELQRLTGDVISRAAFGSNFEEGKKIFEHLKQLTLLTLEAMQTMY 240

Query: 250 LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ 309
           LPGFRF+PT KNRKR+KLNKEIKTMIG+LI+RKE+SM  QEDNV+DLL LLLQSKKKENQ
Sbjct: 241 LPGFRFIPTAKNRKREKLNKEIKTMIGSLILRKENSMSNQEDNVDDLLSLLLQSKKKENQ 300

Query: 310 HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE 369
             G D LTI+EV+EECKQFYLAGQETTAS L WTVIVLAMHS+WQEKARQEVLQ+CGK E
Sbjct: 301 QEG-DELTIEEVVEECKQFYLAGQETTASLLIWTVIVLAMHSDWQEKARQEVLQICGKNE 360

Query: 370 PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD 429
           P+FESL+H KIVTMILYEVLRLYPPVIGQYQHTYAE KIGE+LIPAG+DVTLPTLLIHHD
Sbjct: 361 PSFESLNHFKIVTMILYEVLRLYPPVIGQYQHTYAEVKIGEVLIPAGIDVTLPTLLIHHD 420

Query: 430 PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 489
           PEYWG+DA+QFKPERF+AGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN
Sbjct: 421 PEYWGEDAEQFKPERFAAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 480

Query: 490 FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           FSF+LSPSYAHAP+TVMTLQPQHGAQL LHQL
Sbjct: 481 FSFQLSPSYAHAPHTVMTLQPQHGAQLILHQL 511

BLAST of MS023121 vs. NCBI nr
Match: XP_038879714.1 (cytochrome P450 CYP72A219-like [Benincasa hispida])

HSP 1 Score: 918.3 bits (2372), Expect = 3.0e-263
Identity = 442/512 (86.33%), Postives = 483/512 (94.34%), Query Frame = 0

Query: 10  MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMI 69
           ME+ VFY+L LCLVV+GVAKAFHSIWWRPKLVERQLKRQGIRGS+YKP+VGD KE+VRMI
Sbjct: 1   MEYSVFYSLPLCLVVFGVAKAFHSIWWRPKLVERQLKRQGIRGSSYKPMVGDVKEFVRMI 60

Query: 70  TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG 129
           TEAW+ PMSLDHQIVQRVDPFT VNA+KYGKVS+CWFGT+PRL VKDPEMMKE+LSNK G
Sbjct: 61  TEAWSTPMSLDHQIVQRVDPFTAVNAKKYGKVSMCWFGTNPRLIVKDPEMMKEVLSNKAG 120

Query: 130 SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW 189
           SFEKPPL+P+ILKLT+GLTTLKG+EWAKHRRIINPAFH ERLKGMMPAFSISC NMI++W
Sbjct: 121 SFEKPPLNPLILKLTRGLTTLKGKEWAKHRRIINPAFHHERLKGMMPAFSISCSNMIEEW 180

Query: 190 KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY 249
           K+   +QD VEVDVWPELQRLTADVISRAAF SNFEEGKKIFE LK+LT LTL+AMQT+Y
Sbjct: 181 KEKAANQDSVEVDVWPELQRLTADVISRAAFSSNFEEGKKIFELLKQLTHLTLDAMQTLY 240

Query: 250 LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ 309
           LPGFRF+PT KNR+R+K+NKEIKTMIG+LI+RKE+SM  QEDNV+DLL LLLQSKKKENQ
Sbjct: 241 LPGFRFIPTAKNRRREKMNKEIKTMIGSLILRKENSMSNQEDNVDDLLSLLLQSKKKENQ 300

Query: 310 HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE 369
             G DGLT+DEVMEECKQFYLAGQETTAS LTWTVIVLAMHSEWQEKARQEVLQ+CGK E
Sbjct: 301 QEG-DGLTVDEVMEECKQFYLAGQETTASLLTWTVIVLAMHSEWQEKARQEVLQICGKNE 360

Query: 370 PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD 429
           P+FESL+HLKIVTMIL EVLRLYPPVIGQYQHTYAETKIGE+LIPAGVDVTLPTLLIHHD
Sbjct: 361 PSFESLNHLKIVTMILNEVLRLYPPVIGQYQHTYAETKIGEVLIPAGVDVTLPTLLIHHD 420

Query: 430 PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 489
           P+YWG+DA++FKPERFSAGVSKASKDQLAFF FGWGP+TCIGQNFAMLEAKVAL MILQN
Sbjct: 421 PQYWGEDAEEFKPERFSAGVSKASKDQLAFFAFGWGPKTCIGQNFAMLEAKVALVMILQN 480

Query: 490 FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           FSFELSPSYAHAP+TVMTLQPQHGAQL LHQL
Sbjct: 481 FSFELSPSYAHAPHTVMTLQPQHGAQLKLHQL 511

BLAST of MS023121 vs. NCBI nr
Match: XP_023515452.1 (cytochrome P450 CYP72A219-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 916.0 bits (2366), Expect = 1.5e-262
Identity = 435/519 (83.82%), Postives = 488/519 (94.03%), Query Frame = 0

Query: 3   FVKEKKNMEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDA 62
           FV +   ME+FVFY+L+LCLVV+GVAKAF+SIWWRPKLVERQLKRQG+RGS+YKPLVGDA
Sbjct: 7   FVCKSTKMEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDA 66

Query: 63  KEYVRMITEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKE 122
           KEYV MITEAW+ PM+LDHQI+QRVDPFT V A+KYGK+S+CWFGTSPRL VKDPEMMKE
Sbjct: 67  KEYVSMITEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLMVKDPEMMKE 126

Query: 123 ILSNKVGSFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISC 182
           +LSNK GSFEKPP++P+ILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAF+ISC
Sbjct: 127 VLSNKEGSFEKPPMNPLILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFTISC 186

Query: 183 GNMIKQWKQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTL 242
            NMI++WK+M  +Q+ VEVDVWPE+QRLTADVISRAAFGSNFEEGKK+FE LK+LTL T+
Sbjct: 187 SNMIERWKEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTV 246

Query: 243 EAMQTMYLPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQ 302
           EAMQT+YLPGFRF+PT KNR+R+KLNKEIKT+I ++I+RKE+SM  QE NV+DLLC+LLQ
Sbjct: 247 EAMQTLYLPGFRFIPTAKNRRREKLNKEIKTIIESIILRKENSMSNQEYNVDDLLCMLLQ 306

Query: 303 SKKKENQHGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVL 362
           S+KKENQH G DGLT DEVMEECKQFYLAGQETT+S +TWTVIVLAMHSEWQEKARQEV+
Sbjct: 307 SRKKENQHEG-DGLTTDEVMEECKQFYLAGQETTSSLITWTVIVLAMHSEWQEKARQEVM 366

Query: 363 QVCGKKEPNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLP 422
           Q+CGK EP F++L+HLKIVTMIL EVLRLYPPVI QYQHTYAETKIGE+LIPAGVDVTL 
Sbjct: 367 QICGKNEPTFQNLNHLKIVTMILNEVLRLYPPVIAQYQHTYAETKIGEVLIPAGVDVTLA 426

Query: 423 TLLIHHDPEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVA 482
           TLLIHHDPEYWGDDA++FKPERFSAGVSKASKDQLAFFPFGWGPRTC+GQNFAMLEAK+A
Sbjct: 427 TLLIHHDPEYWGDDAEEFKPERFSAGVSKASKDQLAFFPFGWGPRTCVGQNFAMLEAKIA 486

Query: 483 LAMILQNFSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           LAMILQNFSFELSPSYAHAP+TVMTLQPQHGAQL LHQL
Sbjct: 487 LAMILQNFSFELSPSYAHAPHTVMTLQPQHGAQLKLHQL 524

BLAST of MS023121 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.9e-149
Identity = 255/508 (50.20%), Postives = 359/508 (70.67%), Query Frame = 0

Query: 17  ALSLCLVVYGVA-KAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMITEAWAN 76
           A+++ +VV G A K  + +W  P+ +E  L++QG RG++Y+   GD KE   M  +A   
Sbjct: 14  AVAVAVVVVGWAWKVLNWVWVSPRKLEESLRKQGFRGNSYRLFYGDLKESSEMTRKAKLK 73

Query: 77  PMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVGSFEKPP 136
           P++L    V RV PF     +KYGK S  W G +PR+ + DPE++KEI+  K   F KP 
Sbjct: 74  PINLSDDPVLRVRPFIHQTVKKYGKSSFIWIGPTPRVQIMDPEIIKEIMV-KSYKFNKPK 133

Query: 137 LSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQWKQMGTD 196
            +P++     GL   +GE WAKHR+++NPAFHLERLK M+PA   SC  M+ +W +M + 
Sbjct: 134 RNPLVKLFADGLANHEGELWAKHRKLLNPAFHLERLKCMLPAMYFSCIEMVSKWDKMISK 193

Query: 197 QDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMYLPGFRF 256
               E+DVWP LQRLT+DVIS  AFGS++EEG  +FE       L ++ +Q++Y+PG+ +
Sbjct: 194 DGSRELDVWPFLQRLTSDVISHTAFGSSYEEGNIVFELQTEQAELVMKTLQSVYIPGWSY 253

Query: 257 VPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQHGG--H 316
           +PT +NRK  +++++ ++ + N+I +K  +M+  E + +D+L +LL+S  KE    G  +
Sbjct: 254 LPTKRNRKMKEIDRKTQSCLMNIINKKTKAMQAGEGSTDDILGILLESNLKEQLGQGKKN 313

Query: 317 DGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKEPNFE 376
            G++I EVM ECKQFY AGQETT+  L WT+++L++H  WQ +AR+EVLQ  G  +P+F+
Sbjct: 314 VGMSIQEVMGECKQFYFAGQETTSGLLVWTMVLLSIHPNWQARAREEVLQQFGNAKPDFD 373

Query: 377 SLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHDPEYW 436
           +L+HLKIVTMILYEVLRLYPPV   ++    ET +G++ +PAGV ++LP +++HHD   W
Sbjct: 374 NLNHLKIVTMILYEVLRLYPPVDTLFRRVDQETTLGDITLPAGVQISLPIMILHHDQNIW 433

Query: 437 GDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQNFSFE 496
           GDDA +F PERFS GVSKA+K+Q+ FFPFGWGPR CIGQNFA+LEAK+ALA+ILQ FSFE
Sbjct: 434 GDDAKEFNPERFSEGVSKATKNQVVFFPFGWGPRICIGQNFALLEAKLALAIILQRFSFE 493

Query: 497 LSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           LSPSY HAP TV+T+QPQHGA L LH+L
Sbjct: 494 LSPSYTHAPTTVLTVQPQHGANLILHKL 520

BLAST of MS023121 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.2e-143
Identity = 246/518 (47.49%), Postives = 361/518 (69.69%), Query Frame = 0

Query: 10  MEFFVFYALSLCLVVYGVA---KAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYV 69
           ME  +    S C +V  +    + F+ +W RP+ +E+ L+ QG  G++Y+   GD KE +
Sbjct: 1   MELVLKLISSFCAIVVVILLGWRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKEMI 60

Query: 70  RMITEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSN 129
            M+ EA + P++L   I+ R+ P        YGK S  W G  P + + +P+ +K++LS 
Sbjct: 61  VMLKEAKSKPINLYDDIIPRIIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLS- 120

Query: 130 KVGSFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMI 189
           K   F+KP  +P+   L  G+   +G+ WAKHR++INPAFHLE+LK M+PA  +S   ++
Sbjct: 121 KFYQFQKPRHNPLTKLLATGVADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIV 180

Query: 190 KQWKQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQ 249
            +W++M + + + E+DV P L+ LT+DVISR AFGS++EEG+KIF+  +    L ++A Q
Sbjct: 181 TKWEEMVSTKGQFELDVLPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQASQ 240

Query: 250 TMYLPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQE-DNVEDLLCLLLQSKK 309
           T+YLPG RF+PT +N++  ++ KE+K  + ++I ++  +M   E  + +DLL +LL+S  
Sbjct: 241 TIYLPGMRFLPTKRNKRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDLLGILLESNS 300

Query: 310 KE-NQHGGHD-GLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQ 369
           KE  QHG  + GLT+DEV+EECK F+ AGQETT++ L WT+I+L+ H +WQ++A++EVL+
Sbjct: 301 KEIKQHGNTNFGLTVDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLR 360

Query: 370 VCGKKEPNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPT 429
             G  +P+F+ L+HLK+V MIL EVLRLYPP++   +  Y E K+GE+ +PAGV + LP 
Sbjct: 361 TFGNNKPDFDGLNHLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPI 420

Query: 430 LLIHHDPEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVAL 489
           +L+H+D E WGDDA +F PERFS GV KA+K ++ +FPF WGPR CIGQNFAMLEAK+A+
Sbjct: 421 ILLHYDQEIWGDDAKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAM 480

Query: 490 AMILQNFSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           AMILQ FSF LSPSYAHAP+ ++TLQPQ+GA L LH L
Sbjct: 481 AMILQRFSFVLSPSYAHAPHAIITLQPQYGAHLILHSL 517

BLAST of MS023121 vs. ExPASy Swiss-Prot
Match: Q9LUC5 (Cytochrome P450 72A15 OS=Arabidopsis thaliana OX=3702 GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 7.1e-143
Identity = 239/509 (46.95%), Postives = 354/509 (69.55%), Query Frame = 0

Query: 14  VFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMITEAW 73
           V  ++ L +V + + +    +W++PK++E  L+RQG+ G+ Y PLVGD K+   M++EA 
Sbjct: 8   VTISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEAR 67

Query: 74  ANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVGSFEK 133
           + P+ L   I  RV P+ +   + YG+    WFG  P +T+ DPE +KE+  NKV  F+K
Sbjct: 68  SKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVF-NKVYDFQK 127

Query: 134 PPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQWKQMG 193
           P   P+   + KGL    G++WAKHRRIINPAFH+E++K M+PAF  SC  ++ +W Q+ 
Sbjct: 128 PHTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLV 187

Query: 194 TDQ-DRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMYLPG 253
           +D+    EVDVWP L  +TADVISR AFGS+++EG++IFE    L  L ++A +  ++PG
Sbjct: 188 SDKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPG 247

Query: 254 FRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQHGG 313
           + ++PT  NR+     +EI+ ++  ++ ++  +    E   +DLL +LL+S  ++ +   
Sbjct: 248 YSYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQTE--- 307

Query: 314 HDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKEPNF 373
            +G++ +++MEECK FY AGQETT+  L WT+++L+ H +WQ +AR+EV QV G KEP+ 
Sbjct: 308 GNGMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 367

Query: 374 ESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHDPEY 433
           E L+ LK++TMILYEVLRLYPPV    +  + E K+G+L +P GV ++LP LL+ HD E 
Sbjct: 368 EGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIEL 427

Query: 434 WGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQNFSF 493
           WG+DA +F P+RF  G+SKA+K Q++FFPF WGPR CIGQNFA+LEAK+A+A+IL+ FSF
Sbjct: 428 WGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSF 487

Query: 494 ELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           E+SPSY HAPYTV+T+ PQ GAQL +H+L
Sbjct: 488 EISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of MS023121 vs. ExPASy Swiss-Prot
Match: Q9LUC6 (Cytochrome P450 72A14 OS=Arabidopsis thaliana OX=3702 GN=CYP72A14 PE=2 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 4.6e-142
Identity = 240/509 (47.15%), Postives = 354/509 (69.55%), Query Frame = 0

Query: 14  VFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMITEAW 73
           V ++L++ +V + V +    +W+ PK++ER L+RQG+ G++Y PL+GD K+ + M  EA 
Sbjct: 8   VTFSLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEAT 67

Query: 74  ANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVGSFEK 133
           + P+     I  RV P  +   + +G+ ++ WFG  P +T+ DPE +KE+  NKV  F+K
Sbjct: 68  SKPIKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVF-NKVYDFQK 127

Query: 134 PPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQWKQMG 193
               P+   L  GL +  G++WA+HRRIINPAFHLE++K M+  F  SC  ++ +W ++ 
Sbjct: 128 AHTFPLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLV 187

Query: 194 TDQ-DRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMYLPG 253
           +D+    EVDVWP L  +TADVISR AFGS++ EG +IFE    L  L ++A Q  ++PG
Sbjct: 188 SDKGSSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPG 247

Query: 254 FRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQHGG 313
           + ++PT  NR+     +EI+ ++  +I ++E +  + E   EDLL +LL+S   + +   
Sbjct: 248 YIYLPTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSEDLLGILLESNLGQTE--- 307

Query: 314 HDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKEPNF 373
            +G++ +++MEECK FYLAGQETT+  L WT+++L+ H +WQ +AR+EV QV G K+P+ 
Sbjct: 308 GNGMSTEDMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDT 367

Query: 374 ESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHDPEY 433
           E L+ LK++TMILYEVLRLYPPV+   +  + E K+G+L +P GV ++LP LL+H D E 
Sbjct: 368 EGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTEL 427

Query: 434 WGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQNFSF 493
           WG+DA +FKPERF  G+SKA+K+Q++FFPF WGPR CIGQNF +LEAK+A+++ILQ FSF
Sbjct: 428 WGNDAGEFKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSF 487

Query: 494 ELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           ELSPSY HAPYT++TL PQ GA L LH+L
Sbjct: 488 ELSPSYVHAPYTIITLYPQFGAHLMLHKL 512

BLAST of MS023121 vs. ExPASy Swiss-Prot
Match: W8JWW3 (Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 7.9e-142
Identity = 241/525 (45.90%), Postives = 366/525 (69.71%), Query Frame = 0

Query: 8   KNMEFFVFY----ALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAK 67
           +N E  VFY     L  C  +    K  +  W +PK +E++L++ G++G++Y+ L+GD  
Sbjct: 14  RNREMDVFYRGISVLGTCFFLLLAWKMLNWAWIKPKKLEKRLRQLGLKGNSYRLLIGDLG 73

Query: 68  EYVRMITEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEI 127
           E  +M  EA + P++  + I+ R+ PF     Q +GK    W G +P + + DPE++KE+
Sbjct: 74  ETSQMDKEAKSKPINFTNHIIPRIMPFLHKIIQSHGKNPFIWQGPNPAVIITDPELIKEV 133

Query: 128 LSNKVGSFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCG 187
           L+ K  +++KPP   +   +++G+     ++WAKHRR++NPAFH+E+LK M+PAF +SC 
Sbjct: 134 LT-KNYTYQKPPSDDLGKLISQGVAAYDKDKWAKHRRLLNPAFHIEKLKHMLPAFHLSCS 193

Query: 188 NMIKQWKQM--GTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLT 247
            M+K+W+++   + +   E+DVWP LQ +T+DVISR AFGSN EEGK IF+  K +    
Sbjct: 194 EMLKKWEKLASASAEGSFELDVWPYLQDMTSDVISRTAFGSNLEEGKNIFKLQKEILEHI 253

Query: 248 LEAMQTMYLPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLL 307
           L+ +Q+ Y PG  ++PT + R+  K++KE+ + + N+I+++ ++M+  E + +DLL +LL
Sbjct: 254 LQILQSFYFPGMLYLPTKRIRRMKKISKEVHSSVLNIIMKRVEAMKAGEPSNDDLLGILL 313

Query: 308 QS--KKKENQHGGHD----GLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQE 367
            S  K+ EN +G +     G++ +EV+EECK FY AGQETTA  L WT+++L+ H EWQ 
Sbjct: 314 DSNFKEIENDNGNNSNKNCGMSFEEVIEECKLFYFAGQETTAVLLVWTLLLLSKHPEWQM 373

Query: 368 KARQEVLQVCGKKEPNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPA 427
           +AR EVLQ+ G  EP+F++L+HLK++TM++ EVLRLYPP     +  + +TK+G L +P 
Sbjct: 374 RARDEVLQIFGNSEPDFDALNHLKVITMVINEVLRLYPPGFMMSRVIHEDTKLGSLSLPG 433

Query: 428 GVDVTLPTLLIHHDPEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFA 487
           G+ + LPT+++HHD E WGDDA +FKPERFS G+SKA+K Q  +FPF  GPR CIG NFA
Sbjct: 434 GISIFLPTIMLHHDEELWGDDAKEFKPERFSEGISKATKGQFIYFPFSGGPRICIGNNFA 493

Query: 488 MLEAKVALAMILQNFSFELSPSYAHAPYTVMTLQPQHGAQLTLHQ 521
           MLEAK+ALA+ILQ F+FE+SPSY HAP+T++TLQPQ+GAQL L++
Sbjct: 494 MLEAKMALALILQRFTFEISPSYTHAPHTLVTLQPQYGAQLILYK 537

BLAST of MS023121 vs. ExPASy TrEMBL
Match: A0A6J1D1Q1 (cytochrome P450 CYP72A219-like OS=Momordica charantia OX=3673 GN=LOC111016542 PE=3 SV=1)

HSP 1 Score: 1044.6 bits (2700), Expect = 1.3e-301
Identity = 511/512 (99.80%), Postives = 511/512 (99.80%), Query Frame = 0

Query: 10  MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMI 69
           MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKP VGDAKEYVRMI
Sbjct: 1   MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPWVGDAKEYVRMI 60

Query: 70  TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG 129
           TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG
Sbjct: 61  TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG 120

Query: 130 SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW 189
           SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW
Sbjct: 121 SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW 180

Query: 190 KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY 249
           KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY
Sbjct: 181 KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY 240

Query: 250 LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ 309
           LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ
Sbjct: 241 LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ 300

Query: 310 HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE 369
           HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE
Sbjct: 301 HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE 360

Query: 370 PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD 429
           PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD
Sbjct: 361 PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD 420

Query: 430 PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 489
           PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN
Sbjct: 421 PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 480

Query: 490 FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL
Sbjct: 481 FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 512

BLAST of MS023121 vs. ExPASy TrEMBL
Match: A0A5A7UKX1 (Cytochrome P450 CYP72A219-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001770 PE=3 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 4.0e-266
Identity = 444/512 (86.72%), Postives = 485/512 (94.73%), Query Frame = 0

Query: 10  MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMI 69
           ME+ VFY+L+LCLVV+GVAKAFHSIWW+PKLVER LKRQGIRG+AYKPLVGDAKEYVRMI
Sbjct: 1   MEYSVFYSLALCLVVFGVAKAFHSIWWKPKLVERHLKRQGIRGNAYKPLVGDAKEYVRMI 60

Query: 70  TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG 129
           TEAW+ PM+LDHQIVQRVDPFT VNA+KYGK+S+CWFGTSPRL VKDPEMMKE+L NKVG
Sbjct: 61  TEAWSTPMNLDHQIVQRVDPFTAVNAKKYGKISMCWFGTSPRLIVKDPEMMKEVLMNKVG 120

Query: 130 SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW 189
           SFEKPPLSP+ILKLT+GLTTLKG+EWAKH+RIINPAFHLERLKGMMPAF+ISC NMI+ W
Sbjct: 121 SFEKPPLSPLILKLTRGLTTLKGKEWAKHKRIINPAFHLERLKGMMPAFNISCSNMIEDW 180

Query: 190 KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY 249
           K+   +QD VEVDVWPELQRLT DVISRAAFGSNFEEGKKIFEHLK+LTLLTLEAMQT+Y
Sbjct: 181 KEKAANQDSVEVDVWPELQRLTGDVISRAAFGSNFEEGKKIFEHLKQLTLLTLEAMQTLY 240

Query: 250 LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ 309
           LPGFRF+PT KNRKR+KLNKEIKTMIG+LI+RKE+SM  QEDNV+DLL LLLQSKKKENQ
Sbjct: 241 LPGFRFIPTAKNRKREKLNKEIKTMIGSLILRKENSMSNQEDNVDDLLSLLLQSKKKENQ 300

Query: 310 HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE 369
             G D LTI+EV+EECKQFYLAGQETTAS L WTVIVLAMHS+WQEKARQEVLQ+CGK E
Sbjct: 301 QEG-DELTIEEVVEECKQFYLAGQETTASLLIWTVIVLAMHSDWQEKARQEVLQICGKNE 360

Query: 370 PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD 429
           P+FESL+H KIVTMILYEVLRLYPPVIGQYQHTYAE KIGE+LIPAG+DVTLPTLLIHHD
Sbjct: 361 PSFESLNHFKIVTMILYEVLRLYPPVIGQYQHTYAEVKIGEVLIPAGIDVTLPTLLIHHD 420

Query: 430 PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 489
           PEYWG+DA+QFKPERF+AGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN
Sbjct: 421 PEYWGEDAEQFKPERFAAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 480

Query: 490 FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           FSF+LSPSYAHAP+TVMTLQPQHGAQL LHQL
Sbjct: 481 FSFQLSPSYAHAPHTVMTLQPQHGAQLILHQL 511

BLAST of MS023121 vs. ExPASy TrEMBL
Match: A0A1S3BRD8 (cytochrome P450 CYP72A219-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492359 PE=3 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.5e-265
Identity = 444/512 (86.72%), Postives = 484/512 (94.53%), Query Frame = 0

Query: 10  MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMI 69
           ME+ VFY+L+LCLVV+GVAKAFHSIWW+PKLVER LKRQGIRG+AYKPLVGDAKEYVRMI
Sbjct: 1   MEYSVFYSLALCLVVFGVAKAFHSIWWKPKLVERHLKRQGIRGNAYKPLVGDAKEYVRMI 60

Query: 70  TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG 129
           TEAW+ PM+LDHQIVQRVDPFT VNA+KYGK+S+CWFGTSPRL VKDPEMMKE+L NKVG
Sbjct: 61  TEAWSTPMNLDHQIVQRVDPFTAVNAKKYGKISMCWFGTSPRLIVKDPEMMKEVLMNKVG 120

Query: 130 SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQW 189
           SFEKPPLSP+ILKLT+GLTTLKG+EWAKH+RIINPAFHLERLK MMPAF+ISC NMI+ W
Sbjct: 121 SFEKPPLSPLILKLTRGLTTLKGKEWAKHKRIINPAFHLERLKRMMPAFNISCSNMIEDW 180

Query: 190 KQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMY 249
           K+   +QD VEVDVWPELQRLT DVISRAAFGSNFEEGKKIFEHLK+LTLLTLEAMQTMY
Sbjct: 181 KEKAANQDSVEVDVWPELQRLTGDVISRAAFGSNFEEGKKIFEHLKQLTLLTLEAMQTMY 240

Query: 250 LPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQ 309
           LPGFRF+PT KNRKR+KLNKEIKTMIG+LI+RKE+SM  QEDNV+DLL LLLQSKKKENQ
Sbjct: 241 LPGFRFIPTAKNRKREKLNKEIKTMIGSLILRKENSMSNQEDNVDDLLSLLLQSKKKENQ 300

Query: 310 HGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKE 369
             G D LTI+EV+EECKQFYLAGQETTAS L WTVIVLAMHS+WQEKARQEVLQ+CGK E
Sbjct: 301 QEG-DELTIEEVVEECKQFYLAGQETTASLLIWTVIVLAMHSDWQEKARQEVLQICGKNE 360

Query: 370 PNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHD 429
           P+FESL+H KIVTMILYEVLRLYPPVIGQYQHTYAE KIGE+LIPAG+DVTLPTLLIHHD
Sbjct: 361 PSFESLNHFKIVTMILYEVLRLYPPVIGQYQHTYAEVKIGEVLIPAGIDVTLPTLLIHHD 420

Query: 430 PEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 489
           PEYWG+DA+QFKPERF+AGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN
Sbjct: 421 PEYWGEDAEQFKPERFAAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQN 480

Query: 490 FSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           FSF+LSPSYAHAP+TVMTLQPQHGAQL LHQL
Sbjct: 481 FSFQLSPSYAHAPHTVMTLQPQHGAQLILHQL 511

BLAST of MS023121 vs. ExPASy TrEMBL
Match: A0A1S3BR14 (cytochrome P450 CYP72A219-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492359 PE=3 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 9.3e-263
Identity = 444/525 (84.57%), Postives = 484/525 (92.19%), Query Frame = 0

Query: 10  MEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMI 69
           ME+ VFY+L+LCLVV+GVAKAFHSIWW+PKLVER LKRQGIRG+AYKPLVGDAKEYVRMI
Sbjct: 1   MEYSVFYSLALCLVVFGVAKAFHSIWWKPKLVERHLKRQGIRGNAYKPLVGDAKEYVRMI 60

Query: 70  TEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVG 129
           TEAW+ PM+LDHQIVQRVDPFT VNA+KYGK+S+CWFGTSPRL VKDPEMMKE+L NKVG
Sbjct: 61  TEAWSTPMNLDHQIVQRVDPFTAVNAKKYGKISMCWFGTSPRLIVKDPEMMKEVLMNKVG 120

Query: 130 SFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERL-------------KGMMP 189
           SFEKPPLSP+ILKLT+GLTTLKG+EWAKH+RIINPAFHLERL             K MMP
Sbjct: 121 SFEKPPLSPLILKLTRGLTTLKGKEWAKHKRIINPAFHLERLKEVERILINVLLTKRMMP 180

Query: 190 AFSISCGNMIKQWKQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKR 249
           AF+ISC NMI+ WK+   +QD VEVDVWPELQRLT DVISRAAFGSNFEEGKKIFEHLK+
Sbjct: 181 AFNISCSNMIEDWKEKAANQDSVEVDVWPELQRLTGDVISRAAFGSNFEEGKKIFEHLKQ 240

Query: 250 LTLLTLEAMQTMYLPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDL 309
           LTLLTLEAMQTMYLPGFRF+PT KNRKR+KLNKEIKTMIG+LI+RKE+SM  QEDNV+DL
Sbjct: 241 LTLLTLEAMQTMYLPGFRFIPTAKNRKREKLNKEIKTMIGSLILRKENSMSNQEDNVDDL 300

Query: 310 LCLLLQSKKKENQHGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEK 369
           L LLLQSKKKENQ  G D LTI+EV+EECKQFYLAGQETTAS L WTVIVLAMHS+WQEK
Sbjct: 301 LSLLLQSKKKENQQEG-DELTIEEVVEECKQFYLAGQETTASLLIWTVIVLAMHSDWQEK 360

Query: 370 ARQEVLQVCGKKEPNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAG 429
           ARQEVLQ+CGK EP+FESL+H KIVTMILYEVLRLYPPVIGQYQHTYAE KIGE+LIPAG
Sbjct: 361 ARQEVLQICGKNEPSFESLNHFKIVTMILYEVLRLYPPVIGQYQHTYAEVKIGEVLIPAG 420

Query: 430 VDVTLPTLLIHHDPEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAM 489
           +DVTLPTLLIHHDPEYWG+DA+QFKPERF+AGVSKASKDQLAFFPFGWGPRTCIGQNFAM
Sbjct: 421 IDVTLPTLLIHHDPEYWGEDAEQFKPERFAAGVSKASKDQLAFFPFGWGPRTCIGQNFAM 480

Query: 490 LEAKVALAMILQNFSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           LEAKVALAMILQNFSF+LSPSYAHAP+TVMTLQPQHGAQL LHQL
Sbjct: 481 LEAKVALAMILQNFSFQLSPSYAHAPHTVMTLQPQHGAQLILHQL 524

BLAST of MS023121 vs. ExPASy TrEMBL
Match: A0A6J1HBQ5 (cytochrome P450 CYP72A219-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461826 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 6.0e-262
Identity = 434/519 (83.62%), Postives = 487/519 (93.83%), Query Frame = 0

Query: 3   FVKEKKNMEFFVFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDA 62
           FV +   ME+FVFY+L+LCLVV+GVAKAF+SIWWRPKLVERQLKRQG+RGS+YKPLVGDA
Sbjct: 7   FVCKSTKMEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDA 66

Query: 63  KEYVRMITEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKE 122
           KEYV MITEAW+ PM+LDHQI+QRVDPFT V A+KYGK+S+CWFGTSPRL VK PEMMKE
Sbjct: 67  KEYVSMITEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLMVKAPEMMKE 126

Query: 123 ILSNKVGSFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISC 182
           +LSNK GSFEKPP++P+ILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAF+ISC
Sbjct: 127 VLSNKEGSFEKPPMNPLILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFTISC 186

Query: 183 GNMIKQWKQMGTDQDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTL 242
            NMI++WK+M  +Q+ VEVDVWPE+QRLTADVISRAAFGSNFEEGKK+FE LK+LTL T+
Sbjct: 187 SNMIERWKEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTV 246

Query: 243 EAMQTMYLPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQ 302
           EAMQT+YLPGFRF+PT KNR+R+KLNKEIKT+I ++I+RKE+SM  QEDNV+DLLC+LLQ
Sbjct: 247 EAMQTLYLPGFRFIPTAKNRRREKLNKEIKTIIESIILRKENSMSNQEDNVDDLLCMLLQ 306

Query: 303 SKKKENQHGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVL 362
           S+KKENQ  G DGLT DEVMEECKQFYLAGQETT+S +TWTVIVLAMHSEWQEKARQEVL
Sbjct: 307 SRKKENQQEG-DGLTTDEVMEECKQFYLAGQETTSSLITWTVIVLAMHSEWQEKARQEVL 366

Query: 363 QVCGKKEPNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLP 422
           Q+CGK EP F++L+HLKIVTMIL EVLRLYPPVI QYQHTYAETKIGE+LIPAGVDVTL 
Sbjct: 367 QICGKNEPTFQNLNHLKIVTMILNEVLRLYPPVIAQYQHTYAETKIGEVLIPAGVDVTLA 426

Query: 423 TLLIHHDPEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVA 482
           TLLIHHDPEYWGDDA++FKPERFSAGVSKASKDQLAFFPFGWGPRTC+GQNFAMLEAK+A
Sbjct: 427 TLLIHHDPEYWGDDAEEFKPERFSAGVSKASKDQLAFFPFGWGPRTCVGQNFAMLEAKIA 486

Query: 483 LAMILQNFSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           LAMILQNFSFELSPSYAHAP+TVMTLQPQHGAQL +HQL
Sbjct: 487 LAMILQNFSFELSPSYAHAPHTVMTLQPQHGAQLKVHQL 524

BLAST of MS023121 vs. TAIR 10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15 )

HSP 1 Score: 508.8 bits (1309), Expect = 5.0e-144
Identity = 239/509 (46.95%), Postives = 354/509 (69.55%), Query Frame = 0

Query: 14  VFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMITEAW 73
           V  ++ L +V + + +    +W++PK++E  L+RQG+ G+ Y PLVGD K+   M++EA 
Sbjct: 8   VTISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEAR 67

Query: 74  ANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVGSFEK 133
           + P+ L   I  RV P+ +   + YG+    WFG  P +T+ DPE +KE+  NKV  F+K
Sbjct: 68  SKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVF-NKVYDFQK 127

Query: 134 PPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQWKQMG 193
           P   P+   + KGL    G++WAKHRRIINPAFH+E++K M+PAF  SC  ++ +W Q+ 
Sbjct: 128 PHTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLV 187

Query: 194 TDQ-DRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMYLPG 253
           +D+    EVDVWP L  +TADVISR AFGS+++EG++IFE    L  L ++A +  ++PG
Sbjct: 188 SDKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPG 247

Query: 254 FRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQHGG 313
           + ++PT  NR+     +EI+ ++  ++ ++  +    E   +DLL +LL+S  ++ +   
Sbjct: 248 YSYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQTE--- 307

Query: 314 HDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKEPNF 373
            +G++ +++MEECK FY AGQETT+  L WT+++L+ H +WQ +AR+EV QV G KEP+ 
Sbjct: 308 GNGMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 367

Query: 374 ESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHDPEY 433
           E L+ LK++TMILYEVLRLYPPV    +  + E K+G+L +P GV ++LP LL+ HD E 
Sbjct: 368 EGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIEL 427

Query: 434 WGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQNFSF 493
           WG+DA +F P+RF  G+SKA+K Q++FFPF WGPR CIGQNFA+LEAK+A+A+IL+ FSF
Sbjct: 428 WGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSF 487

Query: 494 ELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           E+SPSY HAPYTV+T+ PQ GAQL +H+L
Sbjct: 488 EISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of MS023121 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 506.1 bits (1302), Expect = 3.3e-143
Identity = 240/509 (47.15%), Postives = 354/509 (69.55%), Query Frame = 0

Query: 14  VFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMITEAW 73
           V ++L++ +V + V +    +W+ PK++ER L+RQG+ G++Y PL+GD K+ + M  EA 
Sbjct: 8   VTFSLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEAT 67

Query: 74  ANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVGSFEK 133
           + P+     I  RV P  +   + +G+ ++ WFG  P +T+ DPE +KE+  NKV  F+K
Sbjct: 68  SKPIKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVF-NKVYDFQK 127

Query: 134 PPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQWKQMG 193
               P+   L  GL +  G++WA+HRRIINPAFHLE++K M+  F  SC  ++ +W ++ 
Sbjct: 128 AHTFPLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLV 187

Query: 194 TDQ-DRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMYLPG 253
           +D+    EVDVWP L  +TADVISR AFGS++ EG +IFE    L  L ++A Q  ++PG
Sbjct: 188 SDKGSSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPG 247

Query: 254 FRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQHGG 313
           + ++PT  NR+     +EI+ ++  +I ++E +  + E   EDLL +LL+S   + +   
Sbjct: 248 YIYLPTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSEDLLGILLESNLGQTE--- 307

Query: 314 HDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKEPNF 373
            +G++ +++MEECK FYLAGQETT+  L WT+++L+ H +WQ +AR+EV QV G K+P+ 
Sbjct: 308 GNGMSTEDMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDT 367

Query: 374 ESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHDPEY 433
           E L+ LK++TMILYEVLRLYPPV+   +  + E K+G+L +P GV ++LP LL+H D E 
Sbjct: 368 EGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTEL 427

Query: 434 WGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQNFSF 493
           WG+DA +FKPERF  G+SKA+K+Q++FFPF WGPR CIGQNF +LEAK+A+++ILQ FSF
Sbjct: 428 WGNDAGEFKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSF 487

Query: 494 ELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           ELSPSY HAPYT++TL PQ GA L LH+L
Sbjct: 488 ELSPSYVHAPYTIITLYPQFGAHLMLHKL 512

BLAST of MS023121 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 503.4 bits (1295), Expect = 2.1e-142
Identity = 249/516 (48.26%), Postives = 353/516 (68.41%), Query Frame = 0

Query: 10  MEFFVFYALS--LCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVR 69
           M F V  AL   + +VV    +    +W +PK++E  LKRQG+ G+ Y PLVGD K  V 
Sbjct: 1   MSFSVVAALPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVD 60

Query: 70  MITEAWANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNK 129
           M+ EA + P+++   I  R+ P  +     +GK    W G  P + + +PE +KE+  NK
Sbjct: 61  MMMEARSKPINVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVF-NK 120

Query: 130 VGSFEKPPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIK 189
           V  FEK    P+I  L  GL + KG++WA HRRIINPAFHLE++K M+PAF   C  ++ 
Sbjct: 121 VNDFEKASTFPLIRLLAGGLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVC 180

Query: 190 QWKQMGTDQDR-VEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQ 249
           QW+++ TD++  +EVDVWP L  +TADVIS  AFGS+++EG++IF+    L  L  +A +
Sbjct: 181 QWEKLFTDKESPLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFK 240

Query: 250 TMYLPGFRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKK 309
             Y+PG RF PT  NR+   +++E+  ++  ++ ++E +    E   +DLL +LL+S  +
Sbjct: 241 KSYIPGSRFYPTKSNRRMKAIDREVDVILRGIVSKREKAREAGEPANDDLLGILLESNSE 300

Query: 310 ENQHGGHDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCG 369
           E+Q    +G+++++VM+ECK FY AGQETT+  L WT+++L+ H +WQ +AR+EV+QV G
Sbjct: 301 ESQ---GNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLG 360

Query: 370 K-KEPNFESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLL 429
           +  +P+ ESL++LK++TMI  EVLRLYPPV    +    E K+GEL +PAG+ + LPT+L
Sbjct: 361 ENNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTIL 420

Query: 430 IHHDPEYWGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAM 489
           +  D E WGDDA  FKPERF  G+SKA+K+Q++FFPFGWGPR CIGQNFAMLEAK+A+A+
Sbjct: 421 VQRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMAL 480

Query: 490 ILQNFSFELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           ILQ FSFELSPSY HAP TVMT +PQ GA L LH+L
Sbjct: 481 ILQKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of MS023121 vs. TAIR 10
Match: AT3G14660.1 (cytochrome P450, family 72, subfamily A, polypeptide 13 )

HSP 1 Score: 496.9 bits (1278), Expect = 2.0e-140
Identity = 238/509 (46.76%), Postives = 347/509 (68.17%), Query Frame = 0

Query: 14  VFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMITEAW 73
           V  ++++ +V + V +    +W +PK++E  L+RQG+ G+ Y PLVGD K    M+ EA 
Sbjct: 8   VTVSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEAR 67

Query: 74  ANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVGSFEK 133
           + P++L   I  R+ P+ +   + +G+    WFG  P +T+ DPE +KE+  NKV  F+K
Sbjct: 68  SKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVF-NKVYDFQK 127

Query: 134 PPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQWKQMG 193
               P+   +  GL +  G++W KHRRIINPAFHLE++K M+PAF  SC  ++ +W ++ 
Sbjct: 128 AHTFPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLV 187

Query: 194 TD-QDRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMYLPG 253
           TD Q   EVD+WP L  +TADVISR AFGS+++EG++IFE    L  L ++A +   +PG
Sbjct: 188 TDKQSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAIIPG 247

Query: 254 FRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQHGG 313
           +R+ PT  NR+     +EIK ++  ++ ++  +    E   +DLL +LL+S   + +   
Sbjct: 248 YRYFPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAPSDDLLGILLESNLGQTK--- 307

Query: 314 HDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKEPNF 373
            +G++ +E+MEECK FY AGQETT   L WT+++L+ H +WQ +AR+EV QV G KEP+ 
Sbjct: 308 GNGMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 367

Query: 374 ESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHDPEY 433
           E L+ LK++TMILYEVLRLYPPV+   +  + E ++G+L +P GV ++LP LLI  D E 
Sbjct: 368 EGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDREL 427

Query: 434 WGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQNFSF 493
           WG+DA +FKP+RF  G+SKA+K+Q++FFPF WGPR CIGQNFA+LEAK+A+ +IL+ FSF
Sbjct: 428 WGNDAGEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKFSF 487

Query: 494 ELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           ELSPSY HAPYTV+T  PQ GA L LH+L
Sbjct: 488 ELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

BLAST of MS023121 vs. TAIR 10
Match: AT3G14630.1 (cytochrome P450, family 72, subfamily A, polypeptide 9 )

HSP 1 Score: 485.7 bits (1249), Expect = 4.6e-137
Identity = 231/509 (45.38%), Postives = 343/509 (67.39%), Query Frame = 0

Query: 14  VFYALSLCLVVYGVAKAFHSIWWRPKLVERQLKRQGIRGSAYKPLVGDAKEYVRMITEAW 73
           V  +L+L +V++ + +    +W +PK++E  L+RQG+ G+ Y PLVGD +    M+ EA 
Sbjct: 4   VIASLALVVVLWCIWRILEWVWLKPKMLESYLRRQGLVGTRYTPLVGDVRRSFSMLKEAR 63

Query: 74  ANPMSLDHQIVQRVDPFTVVNAQKYGKVSVCWFGTSPRLTVKDPEMMKEILSNKVGSFEK 133
           + PM     ++  V P++      YGK    W G  P +T+ +P+++KE+  NK   FEK
Sbjct: 64  SKPMKPTDDLISLVMPYSFHMLNTYGKTFFTWSGPIPAITIMNPQLIKEVY-NKFYDFEK 123

Query: 134 PPLSPVILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFSISCGNMIKQWKQMG 193
               P+   LT GL    G++W KHR+IINPAFH E++K M+P F  SC  ++ +W+++ 
Sbjct: 124 THTFPLTSLLTDGLANADGDKWVKHRKIINPAFHFEKIKNMVPTFYKSCIEVMCEWEKLV 183

Query: 194 TDQ-DRVEVDVWPELQRLTADVISRAAFGSNFEEGKKIFEHLKRLTLLTLEAMQTMYLPG 253
           +D+    E+DVWP +  +T DVISR AFGS+++EG++IF     L  L + A+   Y+P 
Sbjct: 184 SDKGSSCELDVWPWIVNMTGDVISRTAFGSSYKEGQRIFILQAELAHLIILALGKNYIPA 243

Query: 254 FRFVPTVKNRKRDKLNKEIKTMIGNLIIRKEDSMRTQEDNVEDLLCLLLQSKKKENQHGG 313
           +R  PT  NR+   + KEI+ ++  +I  +E +    E   +DLL +LL+S  ++++   
Sbjct: 244 YRHFPTKNNRRMKTIVKEIQVILRGIISHREKARDAGEAPSDDLLGILLKSNSEQSK--- 303

Query: 314 HDGLTIDEVMEECKQFYLAGQETTASWLTWTVIVLAMHSEWQEKARQEVLQVCGKKEPNF 373
            +GL ++E+MEECK FY AGQETT+  L WT+++L+ H +WQ +AR+EV+QV G  +P+ 
Sbjct: 304 GNGLNMEEIMEECKLFYFAGQETTSVLLAWTMVLLSQHQDWQARAREEVMQVFGHNKPDL 363

Query: 374 ESLSHLKIVTMILYEVLRLYPPVIGQYQHTYAETKIGELLIPAGVDVTLPTLLIHHDPEY 433
           + ++ LK++TMI+YEVLRLYPPVI   + T+ E K+G++ +P G+ V +P LLIH D + 
Sbjct: 364 QGINQLKVMTMIIYEVLRLYPPVIQMNRATHKEIKLGDMTLPGGIQVHMPVLLIHRDTKL 423

Query: 434 WGDDADQFKPERFSAGVSKASKDQLAFFPFGWGPRTCIGQNFAMLEAKVALAMILQNFSF 493
           WGDDA +FKPERF  G++KA+K+Q+ F PFGWGPR CIGQNFA+LEAK+ALA+ILQ FSF
Sbjct: 424 WGDDAAEFKPERFKDGIAKATKNQVCFLPFGWGPRICIGQNFALLEAKMALALILQRFSF 483

Query: 494 ELSPSYAHAPYTVMTLQPQHGAQLTLHQL 522
           ELSPSY H+PY V T+ PQ GA L LH+L
Sbjct: 484 ELSPSYVHSPYRVFTIHPQCGAHLILHKL 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147674.12.7e-30199.80cytochrome P450 CYP72A219-like [Momordica charantia][more]
KAA0055790.18.3e-26686.72cytochrome P450 CYP72A219-like isoform X2 [Cucumis melo var. makuwa][more]
XP_008450912.13.2e-26586.72PREDICTED: cytochrome P450 CYP72A219-like isoform X2 [Cucumis melo][more]
XP_038879714.13.0e-26386.33cytochrome P450 CYP72A219-like [Benincasa hispida][more]
XP_023515452.11.5e-26283.82cytochrome P450 CYP72A219-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0S2IHL23.9e-14950.20Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1[more]
H2DH213.2e-14347.49Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
Q9LUC57.1e-14346.95Cytochrome P450 72A15 OS=Arabidopsis thaliana OX=3702 GN=CYP72A15 PE=2 SV=1[more]
Q9LUC64.6e-14247.15Cytochrome P450 72A14 OS=Arabidopsis thaliana OX=3702 GN=CYP72A14 PE=2 SV=1[more]
W8JWW37.9e-14245.90Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D1Q11.3e-30199.80cytochrome P450 CYP72A219-like OS=Momordica charantia OX=3673 GN=LOC111016542 PE... [more]
A0A5A7UKX14.0e-26686.72Cytochrome P450 CYP72A219-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BRD81.5e-26586.72cytochrome P450 CYP72A219-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349235... [more]
A0A1S3BR149.3e-26384.57cytochrome P450 CYP72A219-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349235... [more]
A0A6J1HBQ56.0e-26283.62cytochrome P450 CYP72A219-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G14690.15.0e-14446.95cytochrome P450, family 72, subfamily A, polypeptide 15 [more]
AT3G14680.13.3e-14347.15cytochrome P450, family 72, subfamily A, polypeptide 14 [more]
AT3G14610.12.1e-14248.26cytochrome P450, family 72, subfamily A, polypeptide 7 [more]
AT3G14660.12.0e-14046.76cytochrome P450, family 72, subfamily A, polypeptide 13 [more]
AT3G14630.14.6e-13745.38cytochrome P450, family 72, subfamily A, polypeptide 9 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 383..394
score: 38.62
coord: 469..480
score: 41.91
coord: 460..469
score: 56.37
coord: 331..348
score: 39.15
IPR001128Cytochrome P450PFAMPF00067p450coord: 91..497
e-value: 4.3E-89
score: 299.4
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 210..228
score: 26.21
coord: 459..469
score: 51.84
coord: 95..114
score: 25.6
coord: 320..337
score: 25.11
coord: 340..366
score: 29.45
coord: 469..492
score: 34.81
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 37..521
e-value: 1.3E-114
score: 385.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 94..518
NoneNo IPR availablePANTHERPTHR24282:SF148CYTOCHROME P450 72A15-LIKEcoord: 19..516
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 19..516
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 462..471

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS023121.1MS023121.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen