MS023117 (gene) Bitter gourd (TR) v1

Overview


Name	MS023117
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold78: 271685 .. 272233 (-)
RNA-Seq Expression	MS023117
Synteny	MS023117

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTCTTCTTCTTCTTCAACCCCTTTTACTCAGCCAACGCCCCACCCTGCAAAGTCAAGCTTGCCCATGTTGTATTACGGTCTCGTCGTCGTCGGGACGGCGGCGATTGTACTGGTGGTTTACAATCTCATCGTAATCAGGTGGTGCTCCGACCGCCGTGGCCGAGGATCGCCGGGGATGGCCAGAAGTTATGCGGAGATGATGAGTTCAAGCGGCAGCTTCCAGAGCTCGAGCAGGAATTTGCTGTCCAGCTTCAGGTTCAAGAAGGGAGCCAAGGCGGCGGCGGCAACGGCGGAAGAAGAAGGAAATGGGCTAGAATGTGCGGTTTGTTTGTCGGCGTTTGAGGATGGGGAAGAGGTGAAGAAACTGCCGAGGTGCACCCACATGTTCCACGCCCCCTGCATAGACATGTGGCTTTACTCTCACTCCGATTGCCCCCTCTGCCGTGCGCCGGTGGCGCTCGCAGCGCCGGGGCTGTGTCAGCGCCACACCGCGGCGGAGCAACCGGAAAATTCAGGACATGGGTTGTTGGAATCCGGCGGCTTGGTT

mRNA sequence

TCTTCTTCTTCTTCTTCAACCCCTTTTACTCAGCCAACGCCCCACCCTGCAAAGTCAAGCTTGCCCATGTTGTATTACGGTCTCGTCGTCGTCGGGACGGCGGCGATTGTACTGGTGGTTTACAATCTCATCGTAATCAGGTGGTGCTCCGACCGCCGTGGCCGAGGATCGCCGGGGATGGCCAGAAGTTATGCGGAGATGATGAGTTCAAGCGGCAGCTTCCAGAGCTCGAGCAGGAATTTGCTGTCCAGCTTCAGGTTCAAGAAGGGAGCCAAGGCGGCGGCGGCAACGGCGGAAGAAGAAGGAAATGGGCTAGAATGTGCGGTTTGTTTGTCGGCGTTTGAGGATGGGGAAGAGGTGAAGAAACTGCCGAGGTGCACCCACATGTTCCACGCCCCCTGCATAGACATGTGGCTTTACTCTCACTCCGATTGCCCCCTCTGCCGTGCGCCGGTGGCGCTCGCAGCGCCGGGGCTGTGTCAGCGCCACACCGCGGCGGAGCAACCGGAAAATTCAGGACATGGGTTGTTGGAATCCGGCGGCTTGGTT

Coding sequence (CDS)

TCTTCTTCTTCTTCTTCAACCCCTTTTACTCAGCCAACGCCCCACCCTGCAAAGTCAAGCTTGCCCATGTTGTATTACGGTCTCGTCGTCGTCGGGACGGCGGCGATTGTACTGGTGGTTTACAATCTCATCGTAATCAGGTGGTGCTCCGACCGCCGTGGCCGAGGATCGCCGGGGATGGCCAGAAGTTATGCGGAGATGATGAGTTCAAGCGGCAGCTTCCAGAGCTCGAGCAGGAATTTGCTGTCCAGCTTCAGGTTCAAGAAGGGAGCCAAGGCGGCGGCGGCAACGGCGGAAGAAGAAGGAAATGGGCTAGAATGTGCGGTTTGTTTGTCGGCGTTTGAGGATGGGGAAGAGGTGAAGAAACTGCCGAGGTGCACCCACATGTTCCACGCCCCCTGCATAGACATGTGGCTTTACTCTCACTCCGATTGCCCCCTCTGCCGTGCGCCGGTGGCGCTCGCAGCGCCGGGGCTGTGTCAGCGCCACACCGCGGCGGAGCAACCGGAAAATTCAGGACATGGGTTGTTGGAATCCGGCGGCTTGGTT

Protein sequence

SSSSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSDRRGRGSPGMARSYAEMMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQPENSGHGLLESGGLV

Homology

[Close]

BLAST of MS023117 vs. NCBI nr
Match: XP_022147740.1 (E3 ubiquitin-protein ligase EL5-like [Momordica charantia])

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 177/182 (97.25%), Postives = 177/182 (97.25%), Query Frame = 0


Query: 2   SSSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSDRRGRGSPGMA 61
           S SSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYN IVIRWCSDRRGRGSPGMA
Sbjct: 4   SYSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNFIVIRWCSDRRGRGSPGMA 63

Query: 62  RSYAEMMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEVK 121
           RSYAEMMSSSGSFQSSSRNLLSSFRFKKG KAAAATAEEEGNGLECAVCLSAFEDGEEVK
Sbjct: 64  RSYAEMMSSSGSFQSSSRNLLSSFRFKKGTKAAAATAEEEGNGLECAVCLSAFEDGEEVK 123

Query: 122 KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQPENSGHGLLESGG 181
           KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAL APGLCQ HTAAEQPENSGHGLLESGG
Sbjct: 124 KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAL-APGLCQHHTAAEQPENSGHGLLESGG 183

Query: 182 LV 184
           LV
Sbjct: 184 LV 184

[Close]

BLAST of MS023117 vs. NCBI nr
Match: XP_022968369.1 (RING-H2 finger protein ATL52-like [Cucurbita maxima])

HSP 1 Score: 269.6 bits (688), Expect = 1.9e-68
Identity = 143/185 (77.30%), Postives = 155/185 (83.78%), Query Frame = 0


Query: 4   SSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSDR-RGRGSPGMAR 63
           S+ST   QPT HPAKSSLPMLYYGLVV+GTA IVLV+YNLI+I+WCSD   G+ SP +A 
Sbjct: 4   SASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDNSHGQASPALAG 63

Query: 64  SYAEMMSSSGSFQSSSRNLLSSFRFKKGAKA-AAATAEEEGNGLECAVCLSAFEDGEEVK 123
           SYAEM++SS SFQSS+RNLLSSF+FKKG+ A A AT EEEG G ECAVCLSAFEDGEEVK
Sbjct: 64  SYAEMVNSSRSFQSSNRNLLSSFKFKKGSTAKATATIEEEGIGNECAVCLSAFEDGEEVK 123

Query: 124 KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHT---AAEQPENSGHGLLE 183
           KLPRCTHMFHAPCIDMWLYSHSDCPLCRAP    AP LCQRHT   AAEQ  NSGHGLLE
Sbjct: 124 KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPA--MAPALCQRHTAEDAAEQQVNSGHGLLE 183

[Close]

BLAST of MS023117 vs. NCBI nr
Match: XP_022960602.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita moschata])

HSP 1 Score: 267.7 bits (683), Expect = 7.4e-68
Identity = 142/183 (77.60%), Postives = 155/183 (84.70%), Query Frame = 0


Query: 2   SSSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSD-RRGRGSPGM 61
           S S+S  F QPT HPAKSSLPMLYYGLVV+GTAAIVLV+YN IVI WCSD RRGR SP +
Sbjct: 2   SDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPAL 61

Query: 62  ARSYAEMMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEV 121
           A SYAEM++SS S QSS+RNLL SF+FKKG+ AAAA AEEEGNG EC VCLSAFE+GEEV
Sbjct: 62  AGSYAEMVNSSRSLQSSNRNLLCSFKFKKGS-AAAAAAEEEGNGNECVVCLSAFEEGEEV 121

Query: 122 KKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQPENSGHGLLESG 181
           KKLPRC HMFHA CIDMWL+SH DCPLCRAPVA+  PGL +R TAAEQ  NSGHGL ESG
Sbjct: 122 KKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAV--PGLSKRQTAAEQQGNSGHGLPESG 181

Query: 182 GLV 184
           GLV
Sbjct: 182 GLV 181

[Close]

BLAST of MS023117 vs. NCBI nr
Match: KAG6590024.1 (RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023686.1 RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 267.3 bits (682), Expect = 9.6e-68
Identity = 142/184 (77.17%), Postives = 155/184 (84.24%), Query Frame = 0


Query: 2   SSSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSD-RRGRGSPGM 61
           S S+S  F QPT HPAKSSLPMLYYGLVV+GTAAIVLV+YN IVI WCSD RRGR SP +
Sbjct: 2   SDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPAL 61

Query: 62  ARSYAEMMSSSGSFQSSSRNLLSSFRFKKGAKAAA-ATAEEEGNGLECAVCLSAFEDGEE 121
           A SYAEM++SS S QSS+RNLL SF+FKKG+ AAA A AEEEGNG EC VCLSAFE+GEE
Sbjct: 62  AGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGEE 121

Query: 122 VKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQPENSGHGLLES 181
           VKKLPRC HMFHA CIDMWL+SH DCPLCRAPVA+  PGL +R TAAEQ  NSGHGL ES
Sbjct: 122 VKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAV--PGLSKRQTAAEQQGNSGHGLPES 181

Query: 182 GGLV 184
           GGLV
Sbjct: 182 GGLV 183

[Close]

BLAST of MS023117 vs. NCBI nr
Match: XP_022987479.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita maxima])

HSP 1 Score: 265.4 bits (677), Expect = 3.7e-67
Identity = 140/183 (76.50%), Postives = 154/183 (84.15%), Query Frame = 0


Query: 2   SSSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSD-RRGRGSPGM 61
           S S+S  F+QPT HPAKSSLPMLYYGLVV+GTAAIVLV+YN IVI WCSD RRGR SP +
Sbjct: 2   SDSASNHFSQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPAL 61

Query: 62  ARSYAEMMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEV 121
           A SYAEM++SS S QSS+RNLL SF+FKKG  +AAA  EEEGNG EC VCLSAFE+GEEV
Sbjct: 62  AGSYAEMVNSSRSLQSSNRNLLCSFKFKKG--SAAAAVEEEGNGNECVVCLSAFEEGEEV 121

Query: 122 KKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQPENSGHGLLESG 181
           KKLPRC HMFHA CIDMWL+SH DCPLCRAPVA+  PGL +R TAAEQ  NSGHGL ESG
Sbjct: 122 KKLPRCLHMFHARCIDMWLFSHCDCPLCRAPVAV--PGLSKRQTAAEQQGNSGHGLPESG 180

Query: 182 GLV 184
           GLV
Sbjct: 182 GLV 180

[Close]

BLAST of MS023117 vs. ExPASy Swiss-Prot
Match: Q9LRB7 (E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.1 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.8e-17
Identity = 65/173 (37.57%), Postives = 89/173 (51.45%), Query Frame = 0


Query: 1   SSSSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSDRR------- 60
           SSSSS +P + P    A ++  +     V++  AA+ L    +++  +C +RR       
Sbjct: 16  SSSSSPSPVSAPAGQAAMTAGGIATVAAVLIVFAALTLAF--VLLQCYCDERRRAVTTTS 75

Query: 61  --GRGSPGMARSYAEMMSSSGSFQSSSRNLLSSFR---FKKGAKAAAATAEEE--GNGLE 120
             GRG     R  +      G+       +L S     + +   AAAA  EEE   +G+E
Sbjct: 76  TSGRGRRPRPRRRSGSGGDGGTGGGVDPEVLRSLPVTVYSRSTAAAAAKEEEEEDDDGVE 135

Query: 121 CAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGL 160
           CAVCL+  EDGEE + LPRC H FHA C+DMWL SHS CPLCR  V +  P L
Sbjct: 136 CAVCLAELEDGEEARFLPRCGHGFHAECVDMWLGSHSTCPLCRLTVVVPPPPL 186

[Close]

BLAST of MS023117 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 7.5e-15
Identity = 46/144 (31.94%), Postives = 73/144 (50.69%), Query Frame = 0


Query: 17  AKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSDRRGRGS----------PGMARSYAE 76
           + SS   L   L+ + T+A++LV Y  ++ ++C       S           G   S   
Sbjct: 50  SSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQR 109

Query: 77  MMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEVKKLPRC 136
           + ++      S    ++ +++K G        +   +G +C+VCLS FE+ E ++ LP+C
Sbjct: 110 ISTNGDGLNESMIKSITVYKYKSG--------DGFVDGSDCSVCLSEFEENESLRLLPKC 169

Query: 137 THMFHAPCIDMWLYSHSDCPLCRA 151
            H FH PCID WL SHS+CPLCRA
Sbjct: 170 NHAFHLPCIDTWLKSHSNCPLCRA 185

[Close]

BLAST of MS023117 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-14
Identity = 57/181 (31.49%), Postives = 88/181 (48.62%), Query Frame = 0


Query: 6   STPFTQPT-PHPAKS--SLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSD--------RRG 65
           ST FT P  P  + S  + P+L   ++ +   A +LV Y + VI+ C +        RR 
Sbjct: 23  STTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQIDIFRRRR 82

Query: 66  RGSPGMARSYAEMMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAF 125
           R S             +     S+   +  F+FKK     A   ++  N  EC+VCL+ F
Sbjct: 83  RSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKK-RDVVAGEEDQSKNSQECSVCLNEF 142

Query: 126 EDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQP-ENSG 175
           ++ E+++ +P C H+FH  CID+WL  +++CPLCR  V+  A       +A   P ENS 
Sbjct: 143 QEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSSPRENSP 202

[Close]

BLAST of MS023117 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-14
Identity = 41/95 (43.16%), Postives = 55/95 (57.89%), Query Frame = 0


Query: 77  SSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEVKKLPRCTHMFHAPCID 136
           S+ N ++   FKKG        E   +G EC+VCL+ FE+ E ++ LP+C+H FH  CID
Sbjct: 113 SAINSITVVGFKKG--------EGIIDGTECSVCLNEFEEDESLRLLPKCSHAFHLNCID 172

Query: 137 MWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQPEN 172
            WL SH +CPLCRAPV L      Q      QP++
Sbjct: 173 TWLLSHKNCPLCRAPVLLITEPPHQETETNHQPDS 199

[Close]

BLAST of MS023117 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.7e-14
Identity = 55/171 (32.16%), Postives = 83/171 (48.54%), Query Frame = 0


Query: 9   FTQPTPHP-------AKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSD---------- 68
           F  P P P         +S P+L   ++ +   A +LV Y + VI+ C +          
Sbjct: 15  FPPPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRF 74

Query: 69  ---RRGRGSPGMARSYAEMMSSSGSFQSSSRNLLSSFRFKK------GAKAAAATAEEEG 128
              RR R        Y+  + S G  +S  R  +  F+FKK      G        EEE 
Sbjct: 75  SLSRRRRNDQDPLMVYSPELRSRGLDESVIR-AIPIFKFKKRYDQNDGVFTGEGEEEEEK 134

Query: 129 NGLECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVA 154
              EC+VCLS F+D E+++ +P C+H+FH  CID+WL ++++CPLCR  V+
Sbjct: 135 RSQECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVS 184

[Close]

BLAST of MS023117 vs. ExPASy TrEMBL
Match: A0A6J1D0Z7 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016600 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 7.2e-93
Identity = 177/182 (97.25%), Postives = 177/182 (97.25%), Query Frame = 0


Query: 2   SSSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSDRRGRGSPGMA 61
           S SSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYN IVIRWCSDRRGRGSPGMA
Sbjct: 4   SYSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNFIVIRWCSDRRGRGSPGMA 63

Query: 62  RSYAEMMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEVK 121
           RSYAEMMSSSGSFQSSSRNLLSSFRFKKG KAAAATAEEEGNGLECAVCLSAFEDGEEVK
Sbjct: 64  RSYAEMMSSSGSFQSSSRNLLSSFRFKKGTKAAAATAEEEGNGLECAVCLSAFEDGEEVK 123

Query: 122 KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQPENSGHGLLESGG 181
           KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAL APGLCQ HTAAEQPENSGHGLLESGG
Sbjct: 124 KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAL-APGLCQHHTAAEQPENSGHGLLESGG 183

Query: 182 LV 184
           LV
Sbjct: 184 LV 184

[Close]

BLAST of MS023117 vs. ExPASy TrEMBL
Match: A0A6J1HZG2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467629 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 9.4e-69
Identity = 143/185 (77.30%), Postives = 155/185 (83.78%), Query Frame = 0


Query: 4   SSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSDR-RGRGSPGMAR 63
           S+ST   QPT HPAKSSLPMLYYGLVV+GTA IVLV+YNLI+I+WCSD   G+ SP +A 
Sbjct: 4   SASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDNSHGQASPALAG 63

Query: 64  SYAEMMSSSGSFQSSSRNLLSSFRFKKGAKA-AAATAEEEGNGLECAVCLSAFEDGEEVK 123
           SYAEM++SS SFQSS+RNLLSSF+FKKG+ A A AT EEEG G ECAVCLSAFEDGEEVK
Sbjct: 64  SYAEMVNSSRSFQSSNRNLLSSFKFKKGSTAKATATIEEEGIGNECAVCLSAFEDGEEVK 123

Query: 124 KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHT---AAEQPENSGHGLLE 183
           KLPRCTHMFHAPCIDMWLYSHSDCPLCRAP    AP LCQRHT   AAEQ  NSGHGLLE
Sbjct: 124 KLPRCTHMFHAPCIDMWLYSHSDCPLCRAPA--MAPALCQRHTAEDAAEQQVNSGHGLLE 183

[Close]

BLAST of MS023117 vs. ExPASy TrEMBL
Match: A0A6J1H7W2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461337 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 3.6e-68
Identity = 142/183 (77.60%), Postives = 155/183 (84.70%), Query Frame = 0


Query: 2   SSSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSD-RRGRGSPGM 61
           S S+S  F QPT HPAKSSLPMLYYGLVV+GTAAIVLV+YN IVI WCSD RRGR SP +
Sbjct: 2   SDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPAL 61

Query: 62  ARSYAEMMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEV 121
           A SYAEM++SS S QSS+RNLL SF+FKKG+ AAAA AEEEGNG EC VCLSAFE+GEEV
Sbjct: 62  AGSYAEMVNSSRSLQSSNRNLLCSFKFKKGS-AAAAAAEEEGNGNECVVCLSAFEEGEEV 121

Query: 122 KKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQPENSGHGLLESG 181
           KKLPRC HMFHA CIDMWL+SH DCPLCRAPVA+  PGL +R TAAEQ  NSGHGL ESG
Sbjct: 122 KKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAV--PGLSKRQTAAEQQGNSGHGLPESG 181

Query: 182 GLV 184
           GLV
Sbjct: 182 GLV 181

[Close]

BLAST of MS023117 vs. ExPASy TrEMBL
Match: A0A6J1JJ05 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485020 PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.8e-67
Identity = 140/183 (76.50%), Postives = 154/183 (84.15%), Query Frame = 0


Query: 2   SSSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSD-RRGRGSPGM 61
           S S+S  F+QPT HPAKSSLPMLYYGLVV+GTAAIVLV+YN IVI WCSD RRGR SP +
Sbjct: 2   SDSASNHFSQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPAL 61

Query: 62  ARSYAEMMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEV 121
           A SYAEM++SS S QSS+RNLL SF+FKKG  +AAA  EEEGNG EC VCLSAFE+GEEV
Sbjct: 62  AGSYAEMVNSSRSLQSSNRNLLCSFKFKKG--SAAAAVEEEGNGNECVVCLSAFEEGEEV 121

Query: 122 KKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQPENSGHGLLESG 181
           KKLPRC HMFHA CIDMWL+SH DCPLCRAPVA+  PGL +R TAAEQ  NSGHGL ESG
Sbjct: 122 KKLPRCLHMFHARCIDMWLFSHCDCPLCRAPVAV--PGLSKRQTAAEQQGNSGHGLPESG 180

Query: 182 GLV 184
           GLV
Sbjct: 182 GLV 180

[Close]

BLAST of MS023117 vs. ExPASy TrEMBL
Match: A0A6J1EGS5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434176 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 6.5e-62
Identity = 129/166 (77.71%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 4   SSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCS-DRRGRGSPGMAR 63
           S+ST   QPT HPAKSSLPMLYYGLVV+GTA IVLV+YNLI+I+WCS D  G+ S  +A 
Sbjct: 4   SASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASTALAG 63

Query: 64  SYAEMMSSSGSFQSSSRNLLSSFRFKKGA---KAAAATAEEEGNGLECAVCLSAFEDGEE 123
           SYAEM++SS SFQSS+RNLLSSF+FKKG+    AA AT EEEG G ECAVCLSAFEDGEE
Sbjct: 64  SYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFEDGEE 123

Query: 124 VKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTA 166
           VKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPV   AP LCQRHTA
Sbjct: 124 VKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPV--MAPALCQRHTA 167

[Close]

BLAST of MS023117 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 5.4e-16
Identity = 46/144 (31.94%), Postives = 73/144 (50.69%), Query Frame = 0


Query: 17  AKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSDRRGRGS----------PGMARSYAE 76
           + SS   L   L+ + T+A++LV Y  ++ ++C       S           G   S   
Sbjct: 50  SSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQR 109

Query: 77  MMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEVKKLPRC 136
           + ++      S    ++ +++K G        +   +G +C+VCLS FE+ E ++ LP+C
Sbjct: 110 ISTNGDGLNESMIKSITVYKYKSG--------DGFVDGSDCSVCLSEFEENESLRLLPKC 169

Query: 137 THMFHAPCIDMWLYSHSDCPLCRA 151
            H FH PCID WL SHS+CPLCRA
Sbjct: 170 NHAFHLPCIDTWLKSHSNCPLCRA 185

[Close]

BLAST of MS023117 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 9.1e-16
Identity = 57/181 (31.49%), Postives = 88/181 (48.62%), Query Frame = 0


Query: 6   STPFTQPT-PHPAKS--SLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSD--------RRG 65
           ST FT P  P  + S  + P+L   ++ +   A +LV Y + VI+ C +        RR 
Sbjct: 23  STTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQIDIFRRRR 82

Query: 66  RGSPGMARSYAEMMSSSGSFQSSSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAF 125
           R S             +     S+   +  F+FKK     A   ++  N  EC+VCL+ F
Sbjct: 83  RSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKK-RDVVAGEEDQSKNSQECSVCLNEF 142

Query: 126 EDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQP-ENSG 175
           ++ E+++ +P C H+FH  CID+WL  +++CPLCR  V+  A       +A   P ENS 
Sbjct: 143 QEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSSPRENSP 202

[Close]

BLAST of MS023117 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 9.1e-16
Identity = 41/95 (43.16%), Postives = 55/95 (57.89%), Query Frame = 0


Query: 77  SSRNLLSSFRFKKGAKAAAATAEEEGNGLECAVCLSAFEDGEEVKKLPRCTHMFHAPCID 136
           S+ N ++   FKKG        E   +G EC+VCL+ FE+ E ++ LP+C+H FH  CID
Sbjct: 113 SAINSITVVGFKKG--------EGIIDGTECSVCLNEFEEDESLRLLPKCSHAFHLNCID 172

Query: 137 MWLYSHSDCPLCRAPVALAAPGLCQRHTAAEQPEN 172
            WL SH +CPLCRAPV L      Q      QP++
Sbjct: 173 TWLLSHKNCPLCRAPVLLITEPPHQETETNHQPDS 199

[Close]

BLAST of MS023117 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.2e-15
Identity = 55/171 (32.16%), Postives = 83/171 (48.54%), Query Frame = 0


Query: 9   FTQPTPHP-------AKSSLPMLYYGLVVVGTAAIVLVVYNLIVIRWCSD---------- 68
           F  P P P         +S P+L   ++ +   A +LV Y + VI+ C +          
Sbjct: 15  FPPPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRF 74

Query: 69  ---RRGRGSPGMARSYAEMMSSSGSFQSSSRNLLSSFRFKK------GAKAAAATAEEEG 128
              RR R        Y+  + S G  +S  R  +  F+FKK      G        EEE 
Sbjct: 75  SLSRRRRNDQDPLMVYSPELRSRGLDESVIR-AIPIFKFKKRYDQNDGVFTGEGEEEEEK 134

Query: 129 NGLECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVA 154
              EC+VCLS F+D E+++ +P C+H+FH  CID+WL ++++CPLCR  V+
Sbjct: 135 RSQECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVS 184

[Close]

BLAST of MS023117 vs. TAIR 10
Match: AT2G17450.1 (RING-H2 finger A3A )

HSP 1 Score: 80.5 bits (197), Expect = 1.6e-15
Identity = 37/86 (43.02%), Postives = 50/86 (58.14%), Query Frame = 0


Query: 91  AKAAAATAEEEGNGLECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRA 150
           A++ +  A EEG+  ECA+CL+ F DGEE++ LP C H FH  CID WL S S CP CR 
Sbjct: 86  AESTSGAAAEEGDSTECAICLTDFADGEEIRVLPLCGHSFHVECIDKWLVSRSSCPSCR- 145

Query: 151 PVALAAPGLCQR--HTAAEQPENSGH 175
              +  P  C R  H +  + ++  H
Sbjct: 146 --RILTPVRCDRCGHASTAEMKDQAH 168

The following BLAST results are available for this feature:

BLAST of MS023117 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147740.1	1.5e-92	97.25	E3 ubiquitin-protein ligase EL5-like [Momordica charantia]	[more]
XP_022968369.1	1.9e-68	77.30	RING-H2 finger protein ATL52-like [Cucurbita maxima]	[more]
XP_022960602.1	7.4e-68	77.60	E3 ubiquitin-protein ligase EL5-like [Cucurbita moschata]	[more]
KAG6590024.1	9.6e-68	77.17	RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. sororia] >KA...	[more]
XP_022987479.1	3.7e-67	76.50	E3 ubiquitin-protein ligase EL5-like [Cucurbita maxima]	[more]

BLAST of MS023117 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LRB7	2.8e-17	37.57	E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5....	[more]
Q9LF64	7.5e-15	31.94	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
P93823	1.3e-14	31.49	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]
Q9LX93	1.3e-14	43.16	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
Q9LSW9	1.7e-14	32.16	RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1	[more]

BLAST of MS023117 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D0Z7	7.2e-93	97.25	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101660...	[more]
A0A6J1HZG2	9.4e-69	77.30	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467629 P...	[more]
A0A6J1H7W2	3.6e-68	77.60	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461337...	[more]
A0A6J1JJ05	1.8e-67	76.50	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485020 P...	[more]
A0A6J1EGS5	6.5e-62	77.71	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434176...	[more]

BLAST of MS023117 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17600.1	5.4e-16	31.94	RING/U-box superfamily protein	[more]
AT1G04360.1	9.1e-16	31.49	RING/U-box superfamily protein	[more]
AT5G10380.1	9.1e-16	43.16	RING/U-box superfamily protein	[more]
AT5G43420.1	1.2e-15	32.16	RING/U-box superfamily protein	[more]
AT2G17450.1	1.6e-15	43.02	RING-H2 finger A3A	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 107..148 e-value: 8.7E-6 score: 35.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 106..149 e-value: 6.6E-13 score: 48.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 107..149 score: 12.076194
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 82..155 e-value: 4.6E-21 score: 75.8
None	No IPR available	PANTHER	PTHR45676:SF84	RING-H2 FINGER PROTEIN ATL52-LIKE	coord: 6..178
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 98..155
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 6..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS023117.1	MS023117.1	mRNA