MS023107 (gene) Bitter gourd (TR) v1

BLAST of MS023107 vs. NCBI nr
Match: XP_023529395.1 (protein ALP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 807.4 bits (2084), Expect = 6.8e-230
Identity = 406/490 (82.86%), Postives = 437/490 (89.18%), Query Frame = 0

Query: 1   MEITSFPFLNQEELLPIYSLFSDM-DNFSVNQSSKKRRRQDGDGDDQ----SQSSFDGND 60
           MEI+SFPFLNQ++LLPI++LFS+M DNFSVN S KKRRRQD D DD     +++SFD ++
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNHSPKKRRRQDNDDDDDQTQFNKTSFDDDE 60

Query: 61  LLKLPFWYDQEEKQQHWVMDSNQENANFEADFKREFGVSDESFPQFDIAAKKPRRSAVEN 120
           LLKLPFW+D ++KQQHW+M+            K EF VSDE+  QF    KKPRR+  EN
Sbjct: 61  LLKLPFWFD-DDKQQHWIMEQ-----------KPEFQVSDENLTQF--VPKKPRRATPEN 120

Query: 121 AASASPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKSTFDMICKE 180
             S SP KGGAG   Q RRLWVKDRSKDWWDQCNHP+FPD+EFRRAFRMSK+TFDMICKE
Sbjct: 121 THS-SPVKGGAGT--QHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICKE 180

Query: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240
           LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI
Sbjct: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240

Query: 241 RKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHT 300
           RKVLMPKFLQWP+DSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPK+NVAAYFNKRHT
Sbjct: 241 RKVLMPKFLQWPEDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHT 300

Query: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHIVG 360
           ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSAL+ERAN+GLL DV IVG
Sbjct: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVG 360

Query: 361 NSGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVKLQ 420
           NSG+PL DWLLVPY+ QNLTWTQHAFNEKV EIQ AAKAAF RLKGRW+CLQKRTEVKLQ
Sbjct: 361 NSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQ 420

Query: 421 ELPVVLGACCVLHNICEMRKERFDPELNFEIFDDEMAPE-NGLRSATAIQSRDHIAHNLL 480
           ELPVVLGACCVLHNICEMRKERFDPEL FE FDDEM PE NG+RSA+AIQ+RDHIAHNLL
Sbjct: 421 ELPVVLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLL 473

Query: 481 HHGLAGTGFL 485
           HHGLAGTGFL
Sbjct: 481 HHGLAGTGFL 473

BLAST of MS023107 vs. NCBI nr
Match: XP_022967341.1 (protein ALP1-like [Cucurbita maxima])

HSP 1 Score: 806.2 bits (2081), Expect = 1.5e-229
Identity = 406/489 (83.03%), Postives = 438/489 (89.57%), Query Frame = 0

Query: 1   MEITSFPFLNQEELLPIYSLFSDM-DNFSVNQSSKKRRRQDGDGDDQSQ---SSFDGNDL 60
           MEI+SFPFLNQ++LLPI++LFS+M DNFSVN S KKRRRQD D DDQ+Q   +SFD ++L
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNYSPKKRRRQDDDDDDQTQFNKTSFDDDEL 60

Query: 61  LKLPFWYDQEEKQQHWVMDSNQENANFEADFKREFGVSDESFPQFDIAAKKPRRSAVENA 120
           LKLPFW+D ++KQQHW+M+            K EF V DE+  QF   AKKPRR+  EN 
Sbjct: 61  LKLPFWFD-DDKQQHWIMEQ-----------KPEFQVFDENLTQF--VAKKPRRATPENT 120

Query: 121 ASASPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKSTFDMICKEL 180
            S SPAKGGAG   Q RRLWVKDRSKDWWDQCNHP+FPD+EFRRAFRMSK+TFDMIC+EL
Sbjct: 121 HS-SPAKGGAGT--QHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQEL 180

Query: 181 DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR 240
           DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR
Sbjct: 181 DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR 240

Query: 241 KVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHTE 300
           KVLMPKFLQWP+DSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPK+NVAAYFNKRHTE
Sbjct: 241 KVLMPKFLQWPEDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTE 300

Query: 301 RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHIVGN 360
           RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSAL+ERAN+GLL DV IVGN
Sbjct: 301 RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGN 360

Query: 361 SGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVKLQE 420
           SG+PL DWLLVPY+ QNLTWTQHAFNEKV EIQ AAKAAF RLKGRW+CLQKRTEVKLQE
Sbjct: 361 SGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQE 420

Query: 421 LPVVLGACCVLHNICEMRKERFDPELNFEIFDDEMAPE-NGLRSATAIQSRDHIAHNLLH 480
           LPVVLGACCVLHNICEMRKERFDPEL FE FDDEM PE NG+RSA+AI +RDHI+HNLLH
Sbjct: 421 LPVVLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAILARDHISHNLLH 472

Query: 481 HGLAGTGFL 485
           HGLAGTGFL
Sbjct: 481 HGLAGTGFL 472

BLAST of MS023107 vs. NCBI nr
Match: XP_022933888.1 (protein ALP1-like [Cucurbita moschata] >KAG7021950.1 Protein ALP1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 805.4 bits (2079), Expect = 2.6e-229
Identity = 405/490 (82.65%), Postives = 439/490 (89.59%), Query Frame = 0

Query: 1   MEITSFPFLNQEELLPIYSLFSDM-DNFSVNQSSKKRRRQDGDGDDQ----SQSSFDGND 60
           MEI+SFPFLNQ++LLPI++LFS+M DNFSVNQS KKRRRQ+ D DD     +++SFD ++
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPFWYDQEEKQQHWVMDSNQENANFEADFKREFGVSDESFPQFDIAAKKPRRSAVEN 120
           LLKLPFW+D ++KQQHW+M+            K EF VSDE+  QF    KKPRR+  EN
Sbjct: 61  LLKLPFWFD-DDKQQHWIMEQ-----------KPEFQVSDENLTQF--VPKKPRRATPEN 120

Query: 121 AASASPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKSTFDMICKE 180
             S SPAKGGAG   Q RRLWVKDRSKDWWDQCNHP+FPD+EFRRAFRMSK+TFDMIC+E
Sbjct: 121 THS-SPAKGGAGT--QHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQE 180

Query: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240
           LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI
Sbjct: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240

Query: 241 RKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHT 300
           RKVLMPKFLQWP++SKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPK+NVAAYFNKRHT
Sbjct: 241 RKVLMPKFLQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHT 300

Query: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHIVG 360
           ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSAL+ERAN+GLL DV IVG
Sbjct: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVG 360

Query: 361 NSGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVKLQ 420
           NSG+PL DWLLVPY+ QNLTWTQHAFNEKV EIQ AAKAAF RLKGRW+CLQKRTEVKLQ
Sbjct: 361 NSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQ 420

Query: 421 ELPVVLGACCVLHNICEMRKERFDPELNFEIFDDEMAPE-NGLRSATAIQSRDHIAHNLL 480
           ELPVVLGACCVLHNICEMRKERFDPEL FE FDDEM PE NG+RSA+AIQ+RDHIAHNLL
Sbjct: 421 ELPVVLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLL 473

Query: 481 HHGLAGTGFL 485
           HHGLAGTGFL
Sbjct: 481 HHGLAGTGFL 473

BLAST of MS023107 vs. NCBI nr
Match: KAG6588056.1 (Protein ALP1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 804.3 bits (2076), Expect = 5.8e-229
Identity = 404/490 (82.45%), Postives = 439/490 (89.59%), Query Frame = 0

Query: 1   MEITSFPFLNQEELLPIYSLFSDM-DNFSVNQSSKKRRRQDGDGDDQ----SQSSFDGND 60
           MEI+SFPFLNQ++L+PI++LFS+M DNFSVNQS KKRRRQ+ D DD     +++SFD ++
Sbjct: 1   MEISSFPFLNQDDLVPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPFWYDQEEKQQHWVMDSNQENANFEADFKREFGVSDESFPQFDIAAKKPRRSAVEN 120
           LLKLPFW+D ++KQQHW+M+            K EF VSDE+  QF    KKPRR+  EN
Sbjct: 61  LLKLPFWFD-DDKQQHWIMEQ-----------KPEFQVSDENLTQF--VPKKPRRATPEN 120

Query: 121 AASASPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKSTFDMICKE 180
             S SPAKGGAG   Q RRLWVKDRSKDWWDQCNHP+FPD+EFRRAFRMSK+TFDMIC+E
Sbjct: 121 THS-SPAKGGAGT--QHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQE 180

Query: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240
           LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI
Sbjct: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240

Query: 241 RKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHT 300
           RKVLMPKFLQWP++SKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPK+NVAAYFNKRHT
Sbjct: 241 RKVLMPKFLQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHT 300

Query: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHIVG 360
           ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSAL+ERAN+GLL DV IVG
Sbjct: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVG 360

Query: 361 NSGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVKLQ 420
           NSG+PL DWLLVPY+ QNLTWTQHAFNEKV EIQ AAKAAF RLKGRW+CLQKRTEVKLQ
Sbjct: 361 NSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQ 420

Query: 421 ELPVVLGACCVLHNICEMRKERFDPELNFEIFDDEMAPE-NGLRSATAIQSRDHIAHNLL 480
           ELPVVLGACCVLHNICEMRKERFDPEL FE FDDEM PE NG+RSA+AIQ+RDHIAHNLL
Sbjct: 421 ELPVVLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLL 473

Query: 481 HHGLAGTGFL 485
           HHGLAGTGFL
Sbjct: 481 HHGLAGTGFL 473

BLAST of MS023107 vs. NCBI nr
Match: XP_038880089.1 (protein ALP1-like [Benincasa hispida])

HSP 1 Score: 797.3 bits (2058), Expect = 7.1e-227
Identity = 404/498 (81.12%), Postives = 433/498 (86.95%), Query Frame = 0

Query: 1   MEITSFPFLNQEELLPIYSLFSDMDN--------FSVNQSSKKRRRQDGDGDDQSQ---S 60
           MEI+SFPFLNQEELLPI++LFSDMDN        FSVNQS KKRRR D +GDD SQ    
Sbjct: 1   MEISSFPFLNQEELLPIFNLFSDMDNNHNNTNATFSVNQSPKKRRRSDENGDDHSQFNNI 60

Query: 61  SFDGND--LLKLPFWYDQEEKQQHWVMDSNQENANFEADFKREFGVSDESFPQFDIAAKK 120
           SF  ND  L KLP W+   E Q++W+MDS +         + EF +SD++  QF   +KK
Sbjct: 61  SFTENDEALQKLPCWF---ESQENWIMDSEEPKP------RNEFHLSDQNPTQF---SKK 120

Query: 121 PRRSAVENAASASPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKS 180
           PRR+  EN    SPAK   G G QQRRLWVKDRSKDWWDQCNHP+FPD+EFRRAFRMSKS
Sbjct: 121 PRRTTAEN---GSPAKNPTGGGAQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKS 180

Query: 181 TFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKL 240
           TFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKL
Sbjct: 181 TFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKL 240

Query: 241 VLEVCSAIRKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVA 300
           VLEVCSAIRKVLMPKFLQWPD+SKL KIKQEFESISGIPKVGGSIYTTHIPIIAPK+NVA
Sbjct: 241 VLEVCSAIRKVLMPKFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVA 300

Query: 301 AYFNKRHTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGL 360
           AYFNKRHTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKS L+ERAN+GL
Sbjct: 301 AYFNKRHTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMGL 360

Query: 361 LNDVHIVGNSGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQ 420
           LNDV IVGNSG+PLMDWLLVPY VQNLTWTQH FNEKVGEIQAAAK AF RLKGRWSCLQ
Sbjct: 361 LNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKTAFGRLKGRWSCLQ 420

Query: 421 KRTEVKLQELPVVLGACCVLHNICEMRKERFDPELNFEIFDDEMAPE-NGLRSATAIQSR 480
           KRTEVKLQELPVVLGACCVLHNICE+RKE+FDP+L FE++DDEM PE NGLRS +AIQ+R
Sbjct: 421 KRTEVKLQELPVVLGACCVLHNICEIRKEKFDPDLKFELYDDEMVPENNGLRSVSAIQAR 480

Query: 481 DHIAHNLLHHGLAGTGFL 485
           DHIAHNLLHHGLAGTGFL
Sbjct: 481 DHIAHNLLHHGLAGTGFL 483

BLAST of MS023107 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.4e-36
Identity = 115/362 (31.77%), Postives = 175/362 (48.34%), Query Frame = 0

Query: 104 AAKKPRRSAVENA-ASASPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEF----PDDEF 163
           A KK  R+ +  A A+A+ A   A             +S DWWD  +   +        F
Sbjct: 13  AEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSRRIYGGSTDPKTF 72

Query: 164 RRAFRMSKSTFDMICKELDSTVMKKDTMLRVA----IPVRQRVAVCIWRLATGEPLRLVS 223
              F++S+ TFD IC  + +    K      +    + +  RVAV + RL +GE L ++ 
Sbjct: 73  ESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAVALRRLGSGESLSVIG 132

Query: 224 KRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYT 283
           + FG+  ST  ++      ++ +  +   L WP  SKL +IK +FE ISG+P   G+I  
Sbjct: 133 ETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SKLDEIKSKFEKISGLPNCCGAIDI 192

Query: 284 THIPIIAPKSNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVL 343
           THI +     N+ A         + + ++S+T+Q VVDP   F DV  GWPGS+ DD VL
Sbjct: 193 THIVM-----NLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGSLNDDVVL 252

Query: 344 EKSALYERANLG-LLND------------VHIVGNSGFPLMDWLLVPYAVQNLTWTQHAF 403
           + S  Y+    G  LN              +IVG+SGFPL+ WLL PY  +  +  Q  F
Sbjct: 253 KNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGKPTSLPQTEF 312

Query: 404 NEKVGEIQAAAKAAFARLKGRWSCLQKRTEV-KLQELPVVLGACCVLHN-ICEMRKERFD 442
           N++  E   AA+ A ++LK RW  +     +     LP ++  CC+LHN I +M  +  D
Sbjct: 313 NKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNIIIDMEDQTLD 366

BLAST of MS023107 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 8.6e-34
Identity = 93/314 (29.62%), Postives = 157/314 (50.00%), Query Frame = 0

Query: 143 DWWD----QCNHPEFPDDE---FRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI---- 202
           DWWD    + + P  P DE   F+  FR SK+TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 203 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDS 262
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L+WPD  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 263 KLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHTERNQKTSYSITVQGV 322
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 323 VDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLND-------------VHIVGNS 382
            D    F ++  GWPG M   ++L+ S  ++      + D              ++VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 383 GFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQK-RTEVKLQE 431
            +PL+ WL+ P+   + + +  AFNE+  ++++ A  AF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of MS023107 vs. ExPASy Swiss-Prot
Match: Q96MB7 (Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.1e-17
Identity = 69/261 (26.44%), Postives = 120/261 (45.98%), Query Frame = 0

Query: 175 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 234
           EL    + + T    AI    +V   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGANLSRPTQRSRAISPETQVLAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 235 IRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKR 294
           + +    +F+++P D++ +  +K EF  ++G+P V G +   H+ I AP +   +Y N+ 
Sbjct: 111 LVE-RASQFIRFPADEASIQALKDEFYGLAGMPGVMGVVDCIHVAIKAPNAEDLSYVNR- 170

Query: 295 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHI 354
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+  D  +
Sbjct: 171 ------KGLHSLNCLMVCDIRGTLMTVETNWPGSLQDCAVLQQSSLSSQFEAGMHKDSWL 230

Query: 355 VGNSGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVK 414
           +G+S F L  WL+ P  +   T  ++ +N       +  +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 415 LQELPV----VLGACCVLHNI 431
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of MS023107 vs. ExPASy Swiss-Prot
Match: Q17QR8 (Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.3e-17
Identity = 68/261 (26.05%), Postives = 119/261 (45.59%), Query Frame = 0

Query: 175 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 234
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 235 IRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKR 294
           + +    +F+ +P D++ +  +K EF  ++GIP V G +   H+ I AP +   +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEASVQALKDEFYGLAGIPGVIGVVDCMHVAIKAPNAEDLSYVNR- 170

Query: 295 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHI 354
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+  +  +
Sbjct: 171 ------KGLHSLNCLMVCDIRGALMTVETSWPGSLQDCVVLQQSSLSSQFEAGMHKESWL 230

Query: 355 VGNSGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVK 414
           +G+S F L  WL+ P  +   T  ++ +N       +  +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 415 LQELPV----VLGACCVLHNI 431
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of MS023107 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.3e-17
Identity = 68/261 (26.05%), Postives = 119/261 (45.59%), Query Frame = 0

Query: 175 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 234
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 235 IRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKR 294
           + +    +F+ +P D++ +  +K EF  ++G+P V G++   H+ I AP +   +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYVNR- 170

Query: 295 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHI 354
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+  D  +
Sbjct: 171 ------KGLHSLNCLVVCDIRGALMTVETSWPGSLQDCAVLQQSSLSSQFETGMPKDSWL 230

Query: 355 VGNSGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVK 414
           +G+S F L  WLL P  +   T  ++ +N       +  +     L  R+ CL   ++  
Sbjct: 231 LGDSSFFLHTWLLTPLHIPE-TPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLD-GSKGA 290

Query: 415 LQELPV----VLGACCVLHNI 431
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of MS023107 vs. ExPASy TrEMBL
Match: A0A6J1HRQ5 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111466889 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 7.4e-230
Identity = 406/489 (83.03%), Postives = 438/489 (89.57%), Query Frame = 0

Query: 1   MEITSFPFLNQEELLPIYSLFSDM-DNFSVNQSSKKRRRQDGDGDDQSQ---SSFDGNDL 60
           MEI+SFPFLNQ++LLPI++LFS+M DNFSVN S KKRRRQD D DDQ+Q   +SFD ++L
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNYSPKKRRRQDDDDDDQTQFNKTSFDDDEL 60

Query: 61  LKLPFWYDQEEKQQHWVMDSNQENANFEADFKREFGVSDESFPQFDIAAKKPRRSAVENA 120
           LKLPFW+D ++KQQHW+M+            K EF V DE+  QF   AKKPRR+  EN 
Sbjct: 61  LKLPFWFD-DDKQQHWIMEQ-----------KPEFQVFDENLTQF--VAKKPRRATPENT 120

Query: 121 ASASPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKSTFDMICKEL 180
            S SPAKGGAG   Q RRLWVKDRSKDWWDQCNHP+FPD+EFRRAFRMSK+TFDMIC+EL
Sbjct: 121 HS-SPAKGGAGT--QHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQEL 180

Query: 181 DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR 240
           DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR
Sbjct: 181 DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR 240

Query: 241 KVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHTE 300
           KVLMPKFLQWP+DSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPK+NVAAYFNKRHTE
Sbjct: 241 KVLMPKFLQWPEDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTE 300

Query: 301 RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHIVGN 360
           RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSAL+ERAN+GLL DV IVGN
Sbjct: 301 RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGN 360

Query: 361 SGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVKLQE 420
           SG+PL DWLLVPY+ QNLTWTQHAFNEKV EIQ AAKAAF RLKGRW+CLQKRTEVKLQE
Sbjct: 361 SGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQE 420

Query: 421 LPVVLGACCVLHNICEMRKERFDPELNFEIFDDEMAPE-NGLRSATAIQSRDHIAHNLLH 480
           LPVVLGACCVLHNICEMRKERFDPEL FE FDDEM PE NG+RSA+AI +RDHI+HNLLH
Sbjct: 421 LPVVLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAILARDHISHNLLH 472

Query: 481 HGLAGTGFL 485
           HGLAGTGFL
Sbjct: 481 HGLAGTGFL 472

BLAST of MS023107 vs. ExPASy TrEMBL
Match: A0A6J1F642 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111441167 PE=3 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.3e-229
Identity = 405/490 (82.65%), Postives = 439/490 (89.59%), Query Frame = 0

Query: 1   MEITSFPFLNQEELLPIYSLFSDM-DNFSVNQSSKKRRRQDGDGDDQ----SQSSFDGND 60
           MEI+SFPFLNQ++LLPI++LFS+M DNFSVNQS KKRRRQ+ D DD     +++SFD ++
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPFWYDQEEKQQHWVMDSNQENANFEADFKREFGVSDESFPQFDIAAKKPRRSAVEN 120
           LLKLPFW+D ++KQQHW+M+            K EF VSDE+  QF    KKPRR+  EN
Sbjct: 61  LLKLPFWFD-DDKQQHWIMEQ-----------KPEFQVSDENLTQF--VPKKPRRATPEN 120

Query: 121 AASASPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKSTFDMICKE 180
             S SPAKGGAG   Q RRLWVKDRSKDWWDQCNHP+FPD+EFRRAFRMSK+TFDMIC+E
Sbjct: 121 THS-SPAKGGAGT--QHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQE 180

Query: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240
           LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI
Sbjct: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240

Query: 241 RKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHT 300
           RKVLMPKFLQWP++SKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPK+NVAAYFNKRHT
Sbjct: 241 RKVLMPKFLQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHT 300

Query: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHIVG 360
           ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSAL+ERAN+GLL DV IVG
Sbjct: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVG 360

Query: 361 NSGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVKLQ 420
           NSG+PL DWLLVPY+ QNLTWTQHAFNEKV EIQ AAKAAF RLKGRW+CLQKRTEVKLQ
Sbjct: 361 NSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQ 420

Query: 421 ELPVVLGACCVLHNICEMRKERFDPELNFEIFDDEMAPE-NGLRSATAIQSRDHIAHNLL 480
           ELPVVLGACCVLHNICEMRKERFDPEL FE FDDEM PE NG+RSA+AIQ+RDHIAHNLL
Sbjct: 421 ELPVVLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLL 473

Query: 481 HHGLAGTGFL 485
           HHGLAGTGFL
Sbjct: 481 HHGLAGTGFL 473

BLAST of MS023107 vs. ExPASy TrEMBL
Match: A0A6J1JHN7 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111485185 PE=3 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 7.9e-224
Identity = 392/486 (80.66%), Postives = 428/486 (88.07%), Query Frame = 0

Query: 1   MEITSFPFLNQEELLPIYSLFSDMDNFSVNQSSKKRRRQDGDGDDQSQSSFDGNDLLKLP 60
           MEITS PFLNQ+E LPI + FS++DNFSVNQS KKRRR   +GDD S +   GNDLL LP
Sbjct: 1   MEITSVPFLNQDEFLPISNFFSEIDNFSVNQSLKKRRRPTDNGDDGSGNG--GNDLLNLP 60

Query: 61  FWYD-QEEKQQHWVMDSNQENANFEADFKREFGVSDESFPQFDIAAKKPRRSAVENAASA 120
           FW + ++E+Q+HWVMDS+                      +F + +KKPRR   +N  ++
Sbjct: 61  FWVENRDEQQEHWVMDSD----------------------EFQVLSKKPRRGTPQN-DNS 120

Query: 121 SPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKSTFDMICKELDST 180
           SPAKGGAGA   QRRLWVK+RSKDWWDQCNHP+FPD+EFRRAFRMSK+TFDMIC+ELDST
Sbjct: 121 SPAKGGAGA--HQRRLWVKNRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICRELDST 180

Query: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240
           VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL
Sbjct: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240

Query: 241 MPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHTERNQ 300
           MPKFLQWP++SKL KIKQEFESISGIPKVGG+IYTTHIPIIAPK++VAAYFNKRHTERNQ
Sbjct: 241 MPKFLQWPEESKLTKIKQEFESISGIPKVGGAIYTTHIPIIAPKNSVAAYFNKRHTERNQ 300

Query: 301 KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHIVGNSGF 360
           KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSAL+ERAN+GLLNDV IVGNSG+
Sbjct: 301 KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGY 360

Query: 361 PLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVKLQELPV 420
           PLMDWLLVPY +QNLTWTQHAFNEKVGEIQAAAKAAF RLKGRW+CLQKRTEVKLQELPV
Sbjct: 361 PLMDWLLVPYTLQNLTWTQHAFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPV 420

Query: 421 VLGACCVLHNICEMRKERFDPELNFEIFDDEMAPE-NGLRSATAIQSRDHIAHNLLHHGL 480
           VLGACCVLHNICEMRKERFD EL FE+FDDEM PE NG+RS +AIQ RDHIAHNLLHHGL
Sbjct: 421 VLGACCVLHNICEMRKERFDSELKFEVFDDEMVPESNGVRSVSAIQGRDHIAHNLLHHGL 459

Query: 481 AGTGFL 485
           AGTGFL
Sbjct: 481 AGTGFL 459

BLAST of MS023107 vs. ExPASy TrEMBL
Match: A0A6J1HAH8 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111461585 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.3e-223
Identity = 391/486 (80.45%), Postives = 427/486 (87.86%), Query Frame = 0

Query: 1   MEITSFPFLNQEELLPIYSLFSDMDNFSVNQSSKKRRRQDGDGDDQSQSSFDGNDLLKLP 60
           MEITSFPFLNQ+E LPI + FS+MDNFSVNQS KKRRR   +GDD S +   GNDLL  P
Sbjct: 1   MEITSFPFLNQDEFLPISNFFSEMDNFSVNQSPKKRRRPTDNGDDGSGNG--GNDLLNHP 60

Query: 61  FWYD-QEEKQQHWVMDSNQENANFEADFKREFGVSDESFPQFDIAAKKPRRSAVENAASA 120
           FW + ++E+++HWVMDS+                      +F + +KKPRR   +N  ++
Sbjct: 61  FWVENRDEQREHWVMDSD----------------------EFQVLSKKPRRGTPQN-DNS 120

Query: 121 SPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKSTFDMICKELDST 180
           SPAKGGAGA   QRRLWVK+RSKDWWDQCNHP+FPD+EFRRAFRMSK+TFDMIC+ELDST
Sbjct: 121 SPAKGGAGA--HQRRLWVKNRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICRELDST 180

Query: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240
           VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL
Sbjct: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240

Query: 241 MPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHTERNQ 300
           MPKFLQWP++SKL KIKQEFESISGIPKVGG+IYTTHIPIIAPK++VAAYFNKRHTERNQ
Sbjct: 241 MPKFLQWPEESKLTKIKQEFESISGIPKVGGAIYTTHIPIIAPKNSVAAYFNKRHTERNQ 300

Query: 301 KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLNDVHIVGNSGF 360
           KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDD VLEKSAL+ERAN+GLLNDV IVGNSG+
Sbjct: 301 KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDHVLEKSALFERANMGLLNDVFIVGNSGY 360

Query: 361 PLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVKLQELPV 420
           PLMDWLLVPY +QNLTWTQHAFNEKVGEIQAAAKAAF RLKGRW+CLQKRTEVKLQELPV
Sbjct: 361 PLMDWLLVPYTLQNLTWTQHAFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPV 420

Query: 421 VLGACCVLHNICEMRKERFDPELNFEIFDDEMAPE-NGLRSATAIQSRDHIAHNLLHHGL 480
           VLGACCVLHNICEMRKERFD EL FE+FDDEM PE NG+RS +AIQ RDHIAHNLLHHGL
Sbjct: 421 VLGACCVLHNICEMRKERFDSELKFEVFDDEMVPESNGVRSVSAIQGRDHIAHNLLHHGL 459

Query: 481 AGTGFL 485
           AGTGFL
Sbjct: 481 AGTGFL 459

BLAST of MS023107 vs. ExPASy TrEMBL
Match: A0A0A0LYY2 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589710 PE=3 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 1.8e-220
Identity = 393/497 (79.07%), Postives = 427/497 (85.92%), Query Frame = 0

Query: 1   MEITSFPFLNQEELLPIYSLFSDMDN----FSVNQSSKKRRRQDGDGDDQSQSSF-DGND 60
           MEI+SFPFLNQEE LPI++LFS+MDN    F+VN +SKKRRR D + DD +  SF D ND
Sbjct: 1   MEISSFPFLNQEEFLPIFNLFSEMDNPTATFNVNPTSKKRRRSDPNSDDFNSFSFTDEND 60

Query: 61  ------LLKLPFWYD-QEEKQQHWVMDSNQENANFEADFKREFGVSDESFPQFDIAAKKP 120
                 LLKLP W+D Q E QQ+W+MD+ +           +F +SD+         KKP
Sbjct: 61  DPTADPLLKLPCWFDPQPESQQNWLMDAQKPKPT------NDFHLSDQ-------IPKKP 120

Query: 121 RRSAVENAASASPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKST 180
           RR++ EN +       G G G QQRRLWVKDRSKDWWDQCNHP+FPD+EFRRAFRMSKST
Sbjct: 121 RRASPENPSPVKNTPAG-GGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKST 180

Query: 181 FDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLV 240
           FDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLV
Sbjct: 181 FDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLV 240

Query: 241 LEVCSAIRKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAA 300
           LEVCSAIRKVLMPKFL WPD+SKL KIKQEFESISGIPKVGGSIYTTHIPIIAPK+NVAA
Sbjct: 241 LEVCSAIRKVLMPKFLNWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAA 300

Query: 301 YFNKRHTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLL 360
           YFNKRHTERNQKTSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKS LYERA++G L
Sbjct: 301 YFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGSL 360

Query: 361 NDVHIVGNSGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQK 420
           NDV IVGNSG+PLMDWLLVPY VQNLTWTQH FNEKVGEIQAAAKAAF RLKGRW+CLQK
Sbjct: 361 NDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQK 420

Query: 421 RTEVKLQELPVVLGACCVLHNICEMRKERFDPELNFEIFDDEMAPE-NGLRSATAIQSRD 480
           RTEVKLQELPVVLGACCVLHNICEMRKE+FDPEL FE++DDEM PE NGLRS +AIQ+RD
Sbjct: 421 RTEVKLQELPVVLGACCVLHNICEMRKEKFDPELKFEVYDDEMMPENNGLRSVSAIQARD 480

Query: 481 HIAHNLLHHGLAGTGFL 485
           HIAHNLLHHG+AGTGFL
Sbjct: 481 HIAHNLLHHGIAGTGFL 483

BLAST of MS023107 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 562.0 bits (1447), Expect = 4.7e-160
Identity = 289/496 (58.27%), Postives = 363/496 (73.19%), Query Frame = 0

Query: 37  RRQDGDGDDQSQSS------FDG--------NDLLKLPFWY------DQEEKQQHWVMD- 96
           R +DGD +++ +        FDG        N+   L  ++      ++ EKQ     + 
Sbjct: 7   RNEDGDEEEEEEEEEEVCNVFDGGEVESKRNNETKNLKGFFTSLLLMEEHEKQDQEARNA 66

Query: 97  -SNQENANFEADFKREFGVSDESFPQF-----------DIAAKKPRRS-AVENAASASPA 156
            S +E ++F++++++      + +              DI  KK R S AV + A A+ +
Sbjct: 67  ASRREMSDFQSNYRKRARTMSDYYSDLNDYYADAEESGDINLKKSRVSRAVASVAVAAAS 126

Query: 157 K--------GGAGA-----GGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKSTF 216
           +         G+G+      GQQRRLWVKDRS+ WW++C+  ++P+++F++AFRMSKSTF
Sbjct: 127 EIEAESSEITGSGSVRGTGSGQQRRLWVKDRSRAWWEECSRLDYPEEDFKKAFRMSKSTF 186

Query: 217 DMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVL 276
           ++IC EL+S V K+DT LR AIPVRQRVAVCIWRLATGEPLRLVSK+FGLGISTCHKLVL
Sbjct: 187 ELICDELNSAVAKEDTALRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVL 246

Query: 277 EVCSAIRKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAY 336
           EVC AI+ VLMPK+LQWPDD  L  I++ FES+SGIP V GS+YTTHIPIIAPK +VA+Y
Sbjct: 247 EVCKAIKDVLMPKYLQWPDDESLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASY 306

Query: 337 FNKRHTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANL-GLL 396
           FNKRHTERNQKTSYSIT+Q VV+P GVFTD+CIGWPGSMPDD+VLEKS LY+RAN  GLL
Sbjct: 307 FNKRHTERNQKTSYSITIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLL 366

Query: 397 NDVHIVGNSGFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQK 456
             + + G  G PL+DW+LVPY  QNLTWTQHAFNEK+ E+Q  AK AF RLKGRW+CLQK
Sbjct: 367 KGMWVAGGPGHPLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQK 426

Query: 457 RTEVKLQELPVVLGACCVLHNICEMRKERFDPELNFEIFDDEMAPENGLRSATAIQSRDH 485
           RTEVKLQ+LP VLGACCVLHNICEMR+E+ +PEL  E+ DDE+ PEN LRS  A+++RD 
Sbjct: 427 RTEVKLQDLPTVLGACCVLHNICEMREEKMEPELMVEVIDDEVLPENVLRSVNAMKARDT 486

BLAST of MS023107 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 560.8 bits (1444), Expect = 1.0e-159
Identity = 305/537 (56.80%), Postives = 368/537 (68.53%), Query Frame = 0

Query: 1   MEITSFPF--LNQEELLPIYSLFSDMDN----FSV--------NQSSKKRRRQD------ 60
           MEI+SFPF  L  +E      LF DMD+    F +        N + KKR R+D      
Sbjct: 1   MEISSFPFPYLQDDECSHFLGLFQDMDSSPSTFGLEGFNSNDNNTNQKKRPRKDDEGGGG 60

Query: 61  GDGDDQSQSSFDGN------DLLKLPFWYDQEEKQQ--HWVMDSNQENANFEADFKREFG 120
           G G  +   + +GN      D+L      D+E KQQ   W  +  +E +  EA+ K++  
Sbjct: 61  GGGGTEVLGAVNGNNKAAFGDILATLLLLDEEAKQQQEQWDFEFIKEKSLLEANHKKKVK 120

Query: 121 VSDESFPQFD-----------IAAKKPRRSAVENAASA----------------SPAKGG 180
             D  + Q               +K+ R++AV    SA                +    G
Sbjct: 121 TMDGYYNQMQDHYSAAGETDGSRSKRARKTAVAAVVSAVASGADTTGLAAPVPTADIASG 180

Query: 181 AGAGGQQRRLWVKDRSKDWWDQCNHPEFPDDEFRRAFRMSKSTFDMICKELDSTVMKKDT 240
           +G+G   RRLWVK+R+ DWWD+ + P+FP+DEFRR FRMSKSTF++IC+ELD+TV KK+T
Sbjct: 181 SGSGPSHRRLWVKERTTDWWDRVSRPDFPEDEFRREFRMSKSTFNLICEELDTTVTKKNT 240

Query: 241 MLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQ 300
           MLR AIP  +RV VC+WRLATG PLR VS+RFGLGISTCHKLV+EVC AI  VLMPK+L 
Sbjct: 241 MLRDAIPAPKRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLL 300

Query: 301 WPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHTERNQKTSYSI 360
           WP DS++   K +FES+  IP V GSIYTTHIPIIAPK +VAAYFNKRHTERNQKTSYSI
Sbjct: 301 WPSDSEINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSYSI 360

Query: 361 TVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSAL-YERANLGLLNDVHIVGNSGFPLMDW 420
           TVQGVV+  G+FTDVCIG PGS+ DDQ+LEKS+L  +RA  G+L D  IVGNSGFPL D+
Sbjct: 361 TVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSLSRQRAARGMLRDSWIVGNSGFPLTDY 420

Query: 421 LLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVKLQELPVVLGAC 480
           LLVPY  QNLTWTQHAFNE +GEIQ  A AAF RLKGRW+CLQKRTEVKLQ+LP VLGAC
Sbjct: 421 LLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQKRTEVKLQDLPYVLGAC 480

Query: 481 CVLHNICEMRKERFDPELNFEIFDDEMAPENGLRSATAIQSRDHIAHNLLHHGLAGT 482
           CVLHNICEMRKE   PEL FE+FDD   PEN +RSA+A+ +RDHI+HNLLH GLAGT
Sbjct: 481 CVLHNICEMRKEEMLPELKFEVFDDVAVPENNIRSASAVNTRDHISHNLLHRGLAGT 537

BLAST of MS023107 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 155.6 bits (392), Expect = 1.0e-37
Identity = 115/362 (31.77%), Postives = 175/362 (48.34%), Query Frame = 0

Query: 104 AAKKPRRSAVENA-ASASPAKGGAGAGGQQRRLWVKDRSKDWWDQCNHPEF----PDDEF 163
           A KK  R+ +  A A+A+ A   A             +S DWWD  +   +        F
Sbjct: 13  AEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSRRIYGGSTDPKTF 72

Query: 164 RRAFRMSKSTFDMICKELDSTVMKKDTMLRVA----IPVRQRVAVCIWRLATGEPLRLVS 223
              F++S+ TFD IC  + +    K      +    + +  RVAV + RL +GE L ++ 
Sbjct: 73  ESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAVALRRLGSGESLSVIG 132

Query: 224 KRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYT 283
           + FG+  ST  ++      ++ +  +   L WP  SKL +IK +FE ISG+P   G+I  
Sbjct: 133 ETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SKLDEIKSKFEKISGLPNCCGAIDI 192

Query: 284 THIPIIAPKSNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVL 343
           THI +     N+ A         + + ++S+T+Q VVDP   F DV  GWPGS+ DD VL
Sbjct: 193 THIVM-----NLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGSLNDDVVL 252

Query: 344 EKSALYERANLG-LLND------------VHIVGNSGFPLMDWLLVPYAVQNLTWTQHAF 403
           + S  Y+    G  LN              +IVG+SGFPL+ WLL PY  +  +  Q  F
Sbjct: 253 KNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGKPTSLPQTEF 312

Query: 404 NEKVGEIQAAAKAAFARLKGRWSCLQKRTEV-KLQELPVVLGACCVLHN-ICEMRKERFD 442
           N++  E   AA+ A ++LK RW  +     +     LP ++  CC+LHN I +M  +  D
Sbjct: 313 NKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNIIIDMEDQTLD 366

BLAST of MS023107 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 146.4 bits (368), Expect = 6.1e-35
Identity = 93/314 (29.62%), Postives = 157/314 (50.00%), Query Frame = 0

Query: 143 DWWD----QCNHPEFPDDE---FRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI---- 202
           DWWD    + + P  P DE   F+  FR SK+TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 203 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDS 262
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L+WPD  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 263 KLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKSNVAAYFNKRHTERNQKTSYSITVQGV 322
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 323 VDPAGVFTDVCIGWPGSMPDDQVLEKSALYERANLGLLND-------------VHIVGNS 382
            D    F ++  GWPG M   ++L+ S  ++      + D              ++VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 383 GFPLMDWLLVPYAVQNLTWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQK-RTEVKLQE 431
            +PL+ WL+ P+   + + +  AFNE+  ++++ A  AF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of MS023107 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 100.1 bits (248), Expect = 5.0e-21
Identity = 78/309 (25.24%), Postives = 142/309 (45.95%), Query Frame = 0

Query: 155 DDEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVS 214
           D  +R  + +S   F  +  +L   +    T   +++P    VA+ + RLA G   + ++
Sbjct: 114 DARWRSLYGLSYPVFITVVDKLKPFI----TASNLSLPADYAVAMVLSRLAHGCSAKTLA 173

Query: 215 KRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIY 274
            R+ L      K+   V   +   L P+F++ P    +L +  Q FE ++ +P + G+I 
Sbjct: 174 SRYSLDPYLISKITNMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELTSLPNICGAID 233

Query: 275 TTHIPIIAPKSNVAAYFNKRHTERNQKTSY-------SITVQGVVDPAGVFTDVCIGWPG 334
           +T + +            +R T+ N +  Y       ++ +Q V D   +F DVC+  PG
Sbjct: 234 STPVKL------------RRRTKLNPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPG 293

Query: 335 SMPDDQVLEKSALYERANLG------LLN------DVHIVGNSGFPLMDWLLVPYAVQNL 394
              D      S LY+R   G      ++N        +IVG+  +PL+ +L+ P++    
Sbjct: 294 GEDDSSHFRDSLLYKRLTSGDIVWEKVINIRGHHVRPYIVGDWCYPLLSFLMTPFSPNGS 353

Query: 395 -TWTQHAFNEKVGEIQAAAKAAFARLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEM 443
            T  ++ F+  + + ++    A   LK RW  LQ    V +   P  + ACCVLHN+C++
Sbjct: 354 GTPPENLFDGMLMKGRSVVVEAIGLLKARWKILQS-LNVGVNHAPQTIVACCVLHNLCQI 404