MS023097 (gene) Bitter gourd (TR) v1

Overview


Name	MS023097
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	scaffold78: 93913 .. 95587 (-)
RNA-Seq Expression	MS023097
Synteny	MS023097

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCACAGTGCCTCAGGAAAGGAAGGTCATTGTGAATGACAAGTCTGGTGGAAAGCTTGTGGGAATCCTACATGAAGCTGGATCTGTGGACATTGTTGTCCTCTGTCATGGATTTATGTCATCAAAGGTTACAATCTTTGTTTCATGTGCATTCGTTTCTAAACCCAAAAGTGGCGTTCTATATTTTGTTTGGAAGGTTTGGCTCTTCGCGTCGTGCTGTAGAATTACTTGTTAATGATTGAACTTCGATACGTGCTTGTTGTGTTTTGTTGTTTTCAGGATGATGAAGTTGTGGTGAATCTTGCTGAAGCATTGAACAGAGAAGGAATCAGTTCCTTTCGGTTCGACTTTTCTGGCAATGGGTACGCGTTGTGTAGAATTTGTTAATCAACGGACAGTAATTTGTATAGTTTGAAAATTTCTATCTCAGTGATCATATGTATGGTAAGTCTCTTGGTTCATACAAGGATATGTCAACTATGGGAAAAAACTAATGAGATGTTTGTATTCTTCTGTTTTGACTTTGAAAATTGTCTAATGTTCTATGTTCTAAACTTGAGCTCATTCACTCTTGGATATTTACTCGTTAGAGAAAGCGATGGTTCGTTTCAACTTGGTAATTATGAGAGCGAGGTCGATGATCTACATGCAGTTGTCGAATATTTCAATGTAATAGGTCGTGCAGTACGCTCAATCATTGGCCATAGCAAAGGTCACCCTTCTCCCCCAAAATATACACATATATACATATTTGACGAAGAAACGAAGAGGACTTCTTCTCAAACAACTGGCTTATCTTCTGTATTTGTATGTCTTTATTTTGACTTCTTCTCAAACAACTGGCTTATCTTCTGTATTTGTATGTCTTTATTTTGACTTCTTCTCAAACAACTGGCTTATCTTCTGTATTTGTATGTCTTTATTTTGTACTTTCAGGTGGTGATGTCGTGCTTTTATATGCTTCCAAGTATAAGAATGTTAACATCGTTATCAACGTTTGCGGACGTTATGATATGACGAAAGGCATTGAGAAAAGCTTGGGGGAAAATTACCAGGAAGAGATGGACAAGAAAGGTTTTGTTGATATCAAAAATCCAACAGGTATTTGTTACTTTGATCTCAAATCCTTTACTTTGATTACCTGAATAAAAATCAGACGAACCATTTTCCAGGAACAGTCAGTTATCAAGTGACAGCGGAAAGTTTGATGGAGCGTCTAAATACCGATATGCATCAAGTGTGTCTTCGCATCGATAAAGAATGCAGGCAAGAACACCCATAAATTTCTTCATCTAGAGTATCATTTCTGATGTTATGGGAAGGATTTTTTACTTGTGATCATTGTACAAGTCACCAATAGGGATGGACCGATGGCAATATATGTTCTTATGATTATCAATCATCAGTTAAAGATCTATGTCACCATCATCATCTCGGTTCTCGCATTTACAGCTTCGCCGATGAATAAATGACAATGTTTGTACAGGGTGTTGACAGTCCACGGAACTGACGATGAAATAATCCCTGTCGAAGACGCTCTCGAGTTTGACAAAATCATTCCTAATCACACAATATACATTTTAGAAGGAGCTGACCATAACTACGCCAGAAAGTCGCATCAAGCGGAGTTAGCCACAGTAGTTTTGGAGTTCATCAAAACATCTCTGCAGCAGGAA

mRNA sequence

GCCACAGTGCCTCAGGAAAGGAAGGTCATTGTGAATGACAAGTCTGGTGGAAAGCTTGTGGGAATCCTACATGAAGCTGGATCTGTGGACATTGTTGTCCTCTGTCATGGATTTATGTCATCAAAGGATGATGAAGTTGTGGTGAATCTTGCTGAAGCATTGAACAGAGAAGGAATCAGTTCCTTTCGGTTCGACTTTTCTGGCAATGGAGAAAGCGATGGTTCGTTTCAACTTGGTAATTATGAGAGCGAGGTCGATGATCTACATGCAGTTGTCGAATATTTCAATGTAATAGGTCGTGCAGTACGCTCAATCATTGGCCATAGCAAAGGTGGTGATGTCGTGCTTTTATATGCTTCCAAGTATAAGAATGTTAACATCGTTATCAACGTTTGCGGACGTTATGATATGACGAAAGGCATTGAGAAAAGCTTGGGGGAAAATTACCAGGAAGAGATGGACAAGAAAGGTTTTGTTGATATCAAAAATCCAACAGGAACAGTCAGTTATCAAGTGACAGCGGAAAGTTTGATGGAGCGTCTAAATACCGATATGCATCAAGTGGTGTTGACAGTCCACGGAACTGACGATGAAATAATCCCTGTCGAAGACGCTCTCGAGTTTGACAAAATCATTCCTAATCACACAATATACATTTTAGAAGGAGCTGACCATAACTACGCCAGAAAGTCGCATCAAGCGGAGTTAGCCACAGTAGTTTTGGAGTTCATCAAAACATCTCTGCAGCAGGAA

Coding sequence (CDS)

GCCACAGTGCCTCAGGAAAGGAAGGTCATTGTGAATGACAAGTCTGGTGGAAAGCTTGTGGGAATCCTACATGAAGCTGGATCTGTGGACATTGTTGTCCTCTGTCATGGATTTATGTCATCAAAGGATGATGAAGTTGTGGTGAATCTTGCTGAAGCATTGAACAGAGAAGGAATCAGTTCCTTTCGGTTCGACTTTTCTGGCAATGGAGAAAGCGATGGTTCGTTTCAACTTGGTAATTATGAGAGCGAGGTCGATGATCTACATGCAGTTGTCGAATATTTCAATGTAATAGGTCGTGCAGTACGCTCAATCATTGGCCATAGCAAAGGTGGTGATGTCGTGCTTTTATATGCTTCCAAGTATAAGAATGTTAACATCGTTATCAACGTTTGCGGACGTTATGATATGACGAAAGGCATTGAGAAAAGCTTGGGGGAAAATTACCAGGAAGAGATGGACAAGAAAGGTTTTGTTGATATCAAAAATCCAACAGGAACAGTCAGTTATCAAGTGACAGCGGAAAGTTTGATGGAGCGTCTAAATACCGATATGCATCAAGTGGTGTTGACAGTCCACGGAACTGACGATGAAATAATCCCTGTCGAAGACGCTCTCGAGTTTGACAAAATCATTCCTAATCACACAATATACATTTTAGAAGGAGCTGACCATAACTACGCCAGAAAGTCGCATCAAGCGGAGTTAGCCACAGTAGTTTTGGAGTTCATCAAAACATCTCTGCAGCAGGAA

Protein sequence

ATVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISSFRFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYASKYKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMERLNTDMHQVVLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSHQAELATVVLEFIKTSLQQE

Homology

[Close]

BLAST of MS023097 vs. NCBI nr
Match: XP_022147611.1 (LOW QUALITY PROTEIN: mycophenolic acid acyl-glucuronide esterase, mitochondrial-like [Momordica charantia])

HSP 1 Score: 471.1 bits (1211), Expect = 6.0e-129
Identity = 244/259 (94.21%), Postives = 246/259 (94.98%), Query Frame = 0


Query: 2   TVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISS 61
           +VPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISS
Sbjct: 8   SVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISS 67

Query: 62  FRFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYASK 121
           FRFDFSGNGESDGSFQLGNY SEVDDLHAVV+YFNVIGRAVRSIIGHSKGGDVVLLYASK
Sbjct: 68  FRFDFSGNGESDGSFQLGNYGSEVDDLHAVVQYFNVIGRAVRSIIGHSKGGDVVLLYASK 127

Query: 122 YKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMERL 181
           YKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNP  TVSYQVTAESLMERL
Sbjct: 128 YKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNP--TVSYQVTAESLMERL 187

Query: 182 NTDMHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSH 241
           NTDMHQ          VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSH
Sbjct: 188 NTDMHQXCLRIDKECRVLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSH 247

Query: 242 QAELATVVLEFIKTSLQQE 252
           QAELATVVLEFIKTSLQQE
Sbjct: 248 QAELATVVLEFIKTSLQQE 264

[Close]

BLAST of MS023097 vs. NCBI nr
Match: XP_008450863.1 (PREDICTED: uncharacterized protein LOC103492331 isoform X1 [Cucumis melo])

HSP 1 Score: 397.9 bits (1021), Expect = 6.5e-107
Identity = 196/257 (76.26%), Postives = 229/257 (89.11%), Query Frame = 0


Query: 3   VPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISSF 62
           V QE+KVIVN+K+GGKLVGILHEAGS+ IV++CHG+MSSKDDEV++NLA + ++EGISSF
Sbjct: 15  VSQEKKVIVNEKTGGKLVGILHEAGSLQIVIICHGYMSSKDDEVLLNLATSFDKEGISSF 74

Query: 63  RFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYASKY 122
           RFDFSGNGESDGSFQLGNYESE DDLHA+V+YFN   R+V +IIGHSKGGDVVL+YASKY
Sbjct: 75  RFDFSGNGESDGSFQLGNYESEADDLHAIVQYFNGASRSVCTIIGHSKGGDVVLVYASKY 134

Query: 123 KNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMERLN 182
           K+V+IVINVCGR+DMTKGIEKSLGENY+E M+K+GFVDIK+PTGT++Y+VT ESLMERLN
Sbjct: 135 KDVDIVINVCGRFDMTKGIEKSLGENYEEAMEKQGFVDIKDPTGTLNYKVTQESLMERLN 194

Query: 183 TDMHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSHQ 242
           T+M QV         VLTVHGT+DEIIPV+DA EFDKII NH +YILEGADHNY  + HQ
Sbjct: 195 TNMRQVCLHINQECRVLTVHGTEDEIIPVDDAKEFDKIISNHKLYILEGADHNYTAELHQ 254

Query: 243 AELATVVLEFIKTSLQQ 251
            ELATVVL+FIKTSLQQ
Sbjct: 255 VELATVVLDFIKTSLQQ 271

[Close]

BLAST of MS023097 vs. NCBI nr
Match: XP_011659985.1 (uncharacterized protein LOC101222957 [Cucumis sativus] >KGN66253.1 hypothetical protein Csa_007290 [Cucumis sativus])

HSP 1 Score: 394.8 bits (1013), Expect = 5.5e-106
Identity = 199/261 (76.25%), Postives = 230/261 (88.12%), Query Frame = 0


Query: 1   ATVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGIS 60
           A VPQE+KVIVN+KSGGKLVGILHEAGS+ IV++CHG+MSSKDDEVV+NLA + +REGIS
Sbjct: 15  AMVPQEKKVIVNEKSGGKLVGILHEAGSLRIVIICHGYMSSKDDEVVLNLATSFDREGIS 74

Query: 61  SFRFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYAS 120
           SFRFDFSGNGESDGSFQLGNYESE DDLHA+V+YFN   R V +IIGHSKGGDVVL+YAS
Sbjct: 75  SFRFDFSGNGESDGSFQLGNYESEADDLHAIVQYFNGATRPVCTIIGHSKGGDVVLVYAS 134

Query: 121 KYKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVS-YQVTAESLME 180
           KYK+V+IVINV GR+DMTKGIEKSLGENY+E M+K+GFVDIK+PTGT++ Y+VT ESLME
Sbjct: 135 KYKDVDIVINVSGRFDMTKGIEKSLGENYEEAMEKQGFVDIKDPTGTMNYYRVTRESLME 194

Query: 181 RLNTDMHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARK 240
           RLNT+MHQV         VLTVHGT+D IIPV+DA EFDKII NH +YILEGADHNY  +
Sbjct: 195 RLNTNMHQVCLHINQECRVLTVHGTEDGIIPVDDAKEFDKIISNHKLYILEGADHNYTAE 254

Query: 241 SHQAELATVVLEFIKTSLQQE 252
            HQ +LATVVL+FIKTSLQQ+
Sbjct: 255 LHQVDLATVVLDFIKTSLQQD 275

[Close]

BLAST of MS023097 vs. NCBI nr
Match: XP_038880483.1 (uncharacterized protein LOC120072143 [Benincasa hispida])

HSP 1 Score: 393.7 bits (1010), Expect = 1.2e-105
Identity = 193/260 (74.23%), Postives = 227/260 (87.31%), Query Frame = 0


Query: 1   ATVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGIS 60
           ++V Q +KVIVN+KSGGKLVGILHEAGS+ IV++CHG+MSSKDDEV++NLA + ++EGIS
Sbjct: 10  SSVLQVKKVIVNEKSGGKLVGILHEAGSLQIVIICHGYMSSKDDEVMLNLAASFDKEGIS 69

Query: 61  SFRFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYAS 120
           SFRFDFSGNGESDGSFQLGNYESE DDLHA+V+YFN     V +IIGHSKGGDVVL+YAS
Sbjct: 70  SFRFDFSGNGESDGSFQLGNYESEADDLHAIVQYFNGASHLVCTIIGHSKGGDVVLVYAS 129

Query: 121 KYKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMER 180
           KYK+V I++NVCGRYDMTKGIEKSLGENY+E M+K+GFVDIK+PTG ++Y+VTAESLME 
Sbjct: 130 KYKDVKIIVNVCGRYDMTKGIEKSLGENYEERMEKQGFVDIKDPTGMLNYRVTAESLMEH 189

Query: 181 LNTDMHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKS 240
           LNT+MHQV         VLTVHGT+DEIIPV+DA EFDKII NH +YILEGADHNY  + 
Sbjct: 190 LNTNMHQVCLHIDQQCRVLTVHGTEDEIIPVDDAKEFDKIISNHKLYILEGADHNYTSQL 249

Query: 241 HQAELATVVLEFIKTSLQQE 252
           HQ ELATVVL+FIK SLQQ+
Sbjct: 250 HQVELATVVLDFIKISLQQD 269

[Close]

BLAST of MS023097 vs. NCBI nr
Match: XP_022987794.1 (uncharacterized protein LOC111485234 isoform X1 [Cucurbita maxima])

HSP 1 Score: 382.1 bits (980), Expect = 3.7e-102
Identity = 191/260 (73.46%), Postives = 223/260 (85.77%), Query Frame = 0


Query: 1   ATVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGIS 60
           ATVPQE+KVIVN KSGGKLVGILHEAGS+ IV+LCHG+MS+KDDEVV+NLA A +REGIS
Sbjct: 11  ATVPQEKKVIVNGKSGGKLVGILHEAGSLHIVILCHGYMSTKDDEVVLNLAAAFDREGIS 70

Query: 61  SFRFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYAS 120
           SFRFDFSGNGESDGSFQLGNYE E D+LHA+V+YFN   R+V +IIGHSKG DVVL+YAS
Sbjct: 71  SFRFDFSGNGESDGSFQLGNYEGEADELHAIVQYFNEANRSVCAIIGHSKGADVVLVYAS 130

Query: 121 KYKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMER 180
           KYK+V IV+NV GR+D+T GIEKSLGENY++ MDK+GFVD+K+ TGT  Y+VTAESLMER
Sbjct: 131 KYKDVGIVVNVSGRFDLTTGIEKSLGENYEQGMDKEGFVDVKDSTGTY-YRVTAESLMER 190

Query: 181 LNTDMHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKS 240
           LNTDM Q          VLTVHGT+D +IPV+DA EFDKII NH ++ILEGADHNY  +S
Sbjct: 191 LNTDMSQQCLQIDKECRVLTVHGTEDAVIPVDDAREFDKIISNHKLHILEGADHNYTAES 250

Query: 241 HQAELATVVLEFIKTSLQQE 252
           HQ ELATV+L FIKT+LQQ+
Sbjct: 251 HQVELATVILNFIKTNLQQD 269

[Close]

BLAST of MS023097 vs. ExPASy TrEMBL
Match: A0A6J1D2W5 (LOW QUALITY PROTEIN: mycophenolic acid acyl-glucuronide esterase, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111016493 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.9e-129
Identity = 244/259 (94.21%), Postives = 246/259 (94.98%), Query Frame = 0


Query: 2   TVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISS 61
           +VPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISS
Sbjct: 8   SVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISS 67

Query: 62  FRFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYASK 121
           FRFDFSGNGESDGSFQLGNY SEVDDLHAVV+YFNVIGRAVRSIIGHSKGGDVVLLYASK
Sbjct: 68  FRFDFSGNGESDGSFQLGNYGSEVDDLHAVVQYFNVIGRAVRSIIGHSKGGDVVLLYASK 127

Query: 122 YKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMERL 181
           YKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNP  TVSYQVTAESLMERL
Sbjct: 128 YKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNP--TVSYQVTAESLMERL 187

Query: 182 NTDMHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSH 241
           NTDMHQ          VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSH
Sbjct: 188 NTDMHQXCLRIDKECRVLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSH 247

Query: 242 QAELATVVLEFIKTSLQQE 252
           QAELATVVLEFIKTSLQQE
Sbjct: 248 QAELATVVLEFIKTSLQQE 264

[Close]

BLAST of MS023097 vs. ExPASy TrEMBL
Match: A0A1S3BQW7 (uncharacterized protein LOC103492331 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492331 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 3.1e-107
Identity = 196/257 (76.26%), Postives = 229/257 (89.11%), Query Frame = 0


Query: 3   VPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISSF 62
           V QE+KVIVN+K+GGKLVGILHEAGS+ IV++CHG+MSSKDDEV++NLA + ++EGISSF
Sbjct: 15  VSQEKKVIVNEKTGGKLVGILHEAGSLQIVIICHGYMSSKDDEVLLNLATSFDKEGISSF 74

Query: 63  RFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYASKY 122
           RFDFSGNGESDGSFQLGNYESE DDLHA+V+YFN   R+V +IIGHSKGGDVVL+YASKY
Sbjct: 75  RFDFSGNGESDGSFQLGNYESEADDLHAIVQYFNGASRSVCTIIGHSKGGDVVLVYASKY 134

Query: 123 KNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMERLN 182
           K+V+IVINVCGR+DMTKGIEKSLGENY+E M+K+GFVDIK+PTGT++Y+VT ESLMERLN
Sbjct: 135 KDVDIVINVCGRFDMTKGIEKSLGENYEEAMEKQGFVDIKDPTGTLNYKVTQESLMERLN 194

Query: 183 TDMHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSHQ 242
           T+M QV         VLTVHGT+DEIIPV+DA EFDKII NH +YILEGADHNY  + HQ
Sbjct: 195 TNMRQVCLHINQECRVLTVHGTEDEIIPVDDAKEFDKIISNHKLYILEGADHNYTAELHQ 254

Query: 243 AELATVVLEFIKTSLQQ 251
            ELATVVL+FIKTSLQQ
Sbjct: 255 VELATVVLDFIKTSLQQ 271

[Close]

BLAST of MS023097 vs. ExPASy TrEMBL
Match: A0A0A0LWV0 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589110 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.7e-106
Identity = 199/261 (76.25%), Postives = 230/261 (88.12%), Query Frame = 0


Query: 1   ATVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGIS 60
           A VPQE+KVIVN+KSGGKLVGILHEAGS+ IV++CHG+MSSKDDEVV+NLA + +REGIS
Sbjct: 15  AMVPQEKKVIVNEKSGGKLVGILHEAGSLRIVIICHGYMSSKDDEVVLNLATSFDREGIS 74

Query: 61  SFRFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYAS 120
           SFRFDFSGNGESDGSFQLGNYESE DDLHA+V+YFN   R V +IIGHSKGGDVVL+YAS
Sbjct: 75  SFRFDFSGNGESDGSFQLGNYESEADDLHAIVQYFNGATRPVCTIIGHSKGGDVVLVYAS 134

Query: 121 KYKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVS-YQVTAESLME 180
           KYK+V+IVINV GR+DMTKGIEKSLGENY+E M+K+GFVDIK+PTGT++ Y+VT ESLME
Sbjct: 135 KYKDVDIVINVSGRFDMTKGIEKSLGENYEEAMEKQGFVDIKDPTGTMNYYRVTRESLME 194

Query: 181 RLNTDMHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARK 240
           RLNT+MHQV         VLTVHGT+D IIPV+DA EFDKII NH +YILEGADHNY  +
Sbjct: 195 RLNTNMHQVCLHINQECRVLTVHGTEDGIIPVDDAKEFDKIISNHKLYILEGADHNYTAE 254

Query: 241 SHQAELATVVLEFIKTSLQQE 252
            HQ +LATVVL+FIKTSLQQ+
Sbjct: 255 LHQVDLATVVLDFIKTSLQQD 275

[Close]

BLAST of MS023097 vs. ExPASy TrEMBL
Match: A0A6J1JJU8 (uncharacterized protein LOC111485234 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485234 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.8e-102
Identity = 191/260 (73.46%), Postives = 223/260 (85.77%), Query Frame = 0


Query: 1   ATVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGIS 60
           ATVPQE+KVIVN KSGGKLVGILHEAGS+ IV+LCHG+MS+KDDEVV+NLA A +REGIS
Sbjct: 11  ATVPQEKKVIVNGKSGGKLVGILHEAGSLHIVILCHGYMSTKDDEVVLNLAAAFDREGIS 70

Query: 61  SFRFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYAS 120
           SFRFDFSGNGESDGSFQLGNYE E D+LHA+V+YFN   R+V +IIGHSKG DVVL+YAS
Sbjct: 71  SFRFDFSGNGESDGSFQLGNYEGEADELHAIVQYFNEANRSVCAIIGHSKGADVVLVYAS 130

Query: 121 KYKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMER 180
           KYK+V IV+NV GR+D+T GIEKSLGENY++ MDK+GFVD+K+ TGT  Y+VTAESLMER
Sbjct: 131 KYKDVGIVVNVSGRFDLTTGIEKSLGENYEQGMDKEGFVDVKDSTGTY-YRVTAESLMER 190

Query: 181 LNTDMHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKS 240
           LNTDM Q          VLTVHGT+D +IPV+DA EFDKII NH ++ILEGADHNY  +S
Sbjct: 191 LNTDMSQQCLQIDKECRVLTVHGTEDAVIPVDDAREFDKIISNHKLHILEGADHNYTAES 250

Query: 241 HQAELATVVLEFIKTSLQQE 252
           HQ ELATV+L FIKT+LQQ+
Sbjct: 251 HQVELATVILNFIKTNLQQD 269

[Close]

BLAST of MS023097 vs. ExPASy TrEMBL
Match: A0A6J1JHU7 (uncharacterized protein LOC111485234 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485234 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.8e-102
Identity = 191/260 (73.46%), Postives = 223/260 (85.77%), Query Frame = 0


Query: 1   ATVPQERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGIS 60
           ATVPQE+KVIVN KSGGKLVGILHEAGS+ IV+LCHG+MS+KDDEVV+NLA A +REGIS
Sbjct: 11  ATVPQEKKVIVNGKSGGKLVGILHEAGSLHIVILCHGYMSTKDDEVVLNLAAAFDREGIS 70

Query: 61  SFRFDFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYAS 120
           SFRFDFSGNGESDGSFQLGNYE E D+LHA+V+YFN   R+V +IIGHSKG DVVL+YAS
Sbjct: 71  SFRFDFSGNGESDGSFQLGNYEGEADELHAIVQYFNEANRSVCAIIGHSKGADVVLVYAS 130

Query: 121 KYKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMER 180
           KYK+V IV+NV GR+D+T GIEKSLGENY++ MDK+GFVD+K+ TGT  Y+VTAESLMER
Sbjct: 131 KYKDVGIVVNVSGRFDLTTGIEKSLGENYEQGMDKEGFVDVKDSTGTY-YRVTAESLMER 190

Query: 181 LNTDMHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKS 240
           LNTDM Q          VLTVHGT+D +IPV+DA EFDKII NH ++ILEGADHNY  +S
Sbjct: 191 LNTDMSQQCLQIDKECRVLTVHGTEDAVIPVDDAREFDKIISNHKLHILEGADHNYTAES 250

Query: 241 HQAELATVVLEFIKTSLQQE 252
           HQ ELATV+L FIKT+LQQ+
Sbjct: 251 HQVELATVILNFIKTNLQQD 269

[Close]

BLAST of MS023097 vs. TAIR 10
Match: AT1G29840.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 291.6 bits (745), Expect = 6.1e-79
Identity = 145/250 (58.00%), Postives = 190/250 (76.00%), Query Frame = 0


Query: 6   ERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISSFRFD 65
           ++K+++ + +  KLVG+LHE GS +IVVLCHGF S+K+D+V+ N+A A+ +EGIS+FRFD
Sbjct: 12  QQKIVILNSNNEKLVGLLHETGSTEIVVLCHGFRSTKNDQVMKNVAAAIEKEGISAFRFD 71

Query: 66  FSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYASKYKNV 125
           FSGNGES GSF  GNY  E DDLH+V+ YF  + R V  IIGHSKGGDVVL+YASKY+++
Sbjct: 72  FSGNGESKGSFYFGNYNYEADDLHSVIRYFTNMNRVVPIIIGHSKGGDVVLVYASKYQDI 131

Query: 126 NIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMERLNTDM 185
             VIN+ GRYD+ +GI + LGE+Y E + ++GF+DIK   G   ++VT ESLMERLNTDM
Sbjct: 132 RNVINLSGRYDLKRGIGERLGEDYLERIKQQGFIDIKE--GNAGFRVTEESLMERLNTDM 191

Query: 186 HQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSHQAEL 245
           H+          VLTVHG+ DE+IP+EDA EF KIIPNH + I+EGADH Y +  HQ++L
Sbjct: 192 HEACLKIDKECRVLTVHGSADEVIPLEDAKEFAKIIPNHKLEIVEGADHCYTK--HQSQL 251

Query: 246 ATVVLEFIKT 247
            T V+EFIKT
Sbjct: 252 ITNVMEFIKT 257

[Close]

BLAST of MS023097 vs. TAIR 10
Match: AT3G47590.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 1.5e-77
Identity = 141/251 (56.18%), Postives = 191/251 (76.10%), Query Frame = 0


Query: 5   QERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISSFRF 64
           QE+++++ ++   KLVG+LHE GS DIVVLCHGF S+K ++++ N+A A+ +EGIS+FRF
Sbjct: 57  QEQRIVIPNRHNEKLVGLLHETGSTDIVVLCHGFRSNKSNQIMNNVAAAIQKEGISAFRF 116

Query: 65  DFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYASKYKN 124
           DFSGNGES+GSF  GNY  E DDLH+VV+YF+   R V  I+GHSKGGDVVLLYASKY +
Sbjct: 117 DFSGNGESEGSFYYGNYNHEADDLHSVVQYFSNKNRVVPIILGHSKGGDVVLLYASKYHD 176

Query: 125 VNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMERLNTD 184
           V  VIN+ GRYD+ KGI + LGE++ E + ++GF+D+ +  G   Y+VT +SLM+RL+TD
Sbjct: 177 VRNVINLSGRYDLKKGIRERLGEDFLERIKQQGFIDVGD--GKSGYRVTEKSLMDRLSTD 236

Query: 185 MHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSHQAE 244
           +H+          VLTVHG++DE+IPVEDA EF KIIPNH + I+EGA+H Y    HQ++
Sbjct: 237 IHEACLKIDKECRVLTVHGSEDEVIPVEDAKEFAKIIPNHKLEIVEGANHGYT--EHQSQ 296

Query: 245 LATVVLEFIKT 247
           L + V+EFIKT
Sbjct: 297 LVSTVMEFIKT 303

[Close]

BLAST of MS023097 vs. TAIR 10
Match: AT3G47560.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 4.8e-76
Identity = 139/255 (54.51%), Postives = 191/255 (74.90%), Query Frame = 0


Query: 6   ERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISSFRFD 65
           ++K+++ +     LVG+LHE GS +IVVLCHGF S+K+ E++ N+A A+ REGIS+FRFD
Sbjct: 9   QQKIVILNSHNENLVGLLHETGSTEIVVLCHGFRSNKNFEIMKNVAVAIEREGISAFRFD 68

Query: 66  FSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYASKYKNV 125
           FSGNGES+GSF  GNY  E DDLH+V++YF+ + R V  I+GHSKGGDVVLLYASKY ++
Sbjct: 69  FSGNGESEGSFYYGNYNYEADDLHSVIQYFSNLNRVVTIILGHSKGGDVVLLYASKYHDI 128

Query: 126 NIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMERLNTDM 185
             VIN+ GRYD+ KGI + LGE++ E + ++G++D+K+  G   Y+VT ESLM+RLNTDM
Sbjct: 129 PNVINLSGRYDLKKGIGERLGEDFLERIKQQGYIDVKD--GDSGYRVTEESLMDRLNTDM 188

Query: 186 HQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSHQAEL 245
           H+          VLTVHG+ DE +PVEDA EF KIIPNH + I+EGADH Y   ++Q++L
Sbjct: 189 HEACLKIDKECRVLTVHGSGDETVPVEDAKEFAKIIPNHELQIVEGADHCYT--NYQSQL 248

Query: 246 ATVVLEFIKTSLQQE 252
              V+EFIK+  +++
Sbjct: 249 VLTVMEFIKSHCEEK 259

[Close]

BLAST of MS023097 vs. TAIR 10
Match: AT3G47560.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.0e-69
Identity = 140/306 (45.75%), Postives = 192/306 (62.75%), Query Frame = 0


Query: 6   ERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISSFRFD 65
           ++K+++ +     LVG+LHE GS +IVVLCHGF S+K+ E++ N+A A+ REGIS+FRFD
Sbjct: 9   QQKIVILNSHNENLVGLLHETGSTEIVVLCHGFRSNKNFEIMKNVAVAIEREGISAFRFD 68

Query: 66  FSGNG---------------------------ESDGSFQLGNYESEVDDLHAVVEYFNVI 125
           FSGNG                           ES+GSF  GNY  E DDLH+V++YF+ +
Sbjct: 69  FSGNGYVFLSCIILNKLVFVLTLGLWCFHSFRESEGSFYYGNYNYEADDLHSVIQYFSNL 128

Query: 126 GRAVRSIIGHSKGGDVVLLYASKYKNVNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGF 185
            R V  I+GHSKGGDVVLLYASKY ++  VIN+ GRYD+ KGI + LGE++ E + ++G+
Sbjct: 129 NRVVTIILGHSKGGDVVLLYASKYHDIPNVINLSGRYDLKKGIGERLGEDFLERIKQQGY 188

Query: 186 VDIKN------------------------PTGTVSYQVTAESLMERLNTDMHQV------ 245
           +D+K+                         TG   Y+VT ESLM+RLNTDMH+       
Sbjct: 189 IDVKDGIVIALRFSCTVIRTLVLLSMYWFVTGDSGYRVTEESLMDRLNTDMHEACLKIDK 248

Query: 246 ---VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSHQAELATVVLEFIK 252
              VLTVHG+ DE +PVEDA EF KIIPNH + I+EGADH Y   ++Q++L   V+EFIK
Sbjct: 249 ECRVLTVHGSGDETVPVEDAKEFAKIIPNHELQIVEGADHCYT--NYQSQLVLTVMEFIK 308

[Close]

BLAST of MS023097 vs. TAIR 10
Match: AT5G11910.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 1.6e-66
Identity = 122/250 (48.80%), Postives = 173/250 (69.20%), Query Frame = 0


Query: 5   QERKVIVNDKSGGKLVGILHEAGSVDIVVLCHGFMSSKDDEVVVNLAEALNREGISSFRF 64
           Q ++V++ +  G KLVG+LH+ GS + VV+CHGF SSK+   ++ +A    R  ISSFRF
Sbjct: 20  QHQRVVIENSHGEKLVGVLHDTGSTETVVICHGFRSSKNRIPMLTIASFFERAMISSFRF 79

Query: 65  DFSGNGESDGSFQLGNYESEVDDLHAVVEYFNVIGRAVRSIIGHSKGGDVVLLYASKYKN 124
           DF+GNGES GSFQ GNY  EV+DL +V+++   + R + +IIGHSKGG+VVLLYA+KY +
Sbjct: 80  DFAGNGESQGSFQYGNYRREVEDLRSVLQHLRGVNRVISAIIGHSKGGNVVLLYAAKYND 139

Query: 125 VNIVINVCGRYDMTKGIEKSLGENYQEEMDKKGFVDIKNPTGTVSYQVTAESLMERLNTD 184
           V  V+N+ GR+ + +GIE  LG++Y + +   GF+D+ N  G   Y+VT ESLM+RL T+
Sbjct: 140 VQTVVNISGRFFLDRGIEFRLGKDYFKRIKDNGFIDVSNRKGKFEYRVTEESLMDRLTTN 199

Query: 185 MHQV---------VLTVHGTDDEIIPVEDALEFDKIIPNHTIYILEGADHNYARKSHQAE 244
            H+          VLTVHG++D I+ V +A EF K I NH +Y++EGADH +   SHQ +
Sbjct: 200 AHEACLSIRENCRVLTVHGSNDRIVHVTEASEFAKQIKNHKLYVIEGADHEFT--SHQHQ 259

Query: 245 LATVVLEFIK 246
           LA++VL F K
Sbjct: 260 LASIVLSFFK 267

The following BLAST results are available for this feature:

BLAST of MS023097 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147611.1	6.0e-129	94.21	LOW QUALITY PROTEIN: mycophenolic acid acyl-glucuronide esterase, mitochondrial-...	[more]
XP_008450863.1	6.5e-107	76.26	PREDICTED: uncharacterized protein LOC103492331 isoform X1 [Cucumis melo]	[more]
XP_011659985.1	5.5e-106	76.25	uncharacterized protein LOC101222957 [Cucumis sativus] >KGN66253.1 hypothetical ...	[more]
XP_038880483.1	1.2e-105	74.23	uncharacterized protein LOC120072143 [Benincasa hispida]	[more]
XP_022987794.1	3.7e-102	73.46	uncharacterized protein LOC111485234 isoform X1 [Cucurbita maxima]	[more]

BLAST of MS023097 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS023097 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2W5	2.9e-129	94.21	LOW QUALITY PROTEIN: mycophenolic acid acyl-glucuronide esterase, mitochondrial-...	[more]
A0A1S3BQW7	3.1e-107	76.26	uncharacterized protein LOC103492331 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0LWV0	2.7e-106	76.25	Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589110...	[more]
A0A6J1JJU8	1.8e-102	73.46	uncharacterized protein LOC111485234 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1JHU7	1.8e-102	73.46	uncharacterized protein LOC111485234 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of MS023097 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G29840.1	6.1e-79	58.00	alpha/beta-Hydrolases superfamily protein	[more]
AT3G47590.1	1.5e-77	56.18	alpha/beta-Hydrolases superfamily protein	[more]
AT3G47560.1	4.8e-76	54.51	alpha/beta-Hydrolases superfamily protein	[more]
AT3G47560.2	2.0e-69	45.75	alpha/beta-Hydrolases superfamily protein	[more]
AT5G11910.1	1.6e-66	48.80	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022742	Serine aminopeptidase, S33	PFAM	PF12146	Hydrolase_4	coord: 31..131 e-value: 6.4E-13 score: 48.5
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 3..251 e-value: 8.9E-49 score: 168.1
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 6..248
None	No IPR available	PANTHER	PTHR42886:SF53	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 5..250
None	No IPR available	PANTHER	PTHR42886	RE40534P-RELATED	coord: 5..250

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS023097.1	MS023097.1	mRNA