MS023025 (gene) Bitter gourd (TR) v1

Overview
NameMS023025
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontranscription factor bHLH130
Locationscaffold357: 698339 .. 700114 (-)
RNA-Seq ExpressionMS023025
SyntenyMS023025
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCGAATACCCACCAGAGTTTTCAGCAACCCAATTCGGCGTTGCTCCGATTTCGGTCTGCTCCCAGTTCTCTGTTCGCTGATTTCGCTCATGGGGTTGATTCGAAGCGGTTGAATCACTTCGAGGGCTCGGAATCCGAGCGATTTGTTTCCAGATTTCGCGGCGGCGGTGGCGGTGGAAATAGTAATGACTCCGAATCTACGGCGGCCGGAAACTACTCCTCTGCCCTGCCGCCGCATTACCCCCGCCACTCCTCCGCCGTGAGTTCTTCTTCTTGTTCCTCGTCTTCGGCTTCTTCTGCTACAACTTCTTCTTCTTCTTCTTCAATGAGTTGCTCTTATGGGTTGCTGGGTTCTAATCTTGCTCGCCAGAATAGCTCTCCTCCTGGAGTTTTCTCTCACCTCAATCAAAACGGTAATTCACTTTTCTCCTCCATTACTCTGTGAAGCTTTGAGTGCATTAATGGCAGAAGTCAACTTCCCCTGTGAATTTCCATTTCATATCGTCAATAATATGATGGGAAAATTCGATCTGATATATATACTGATATGTAGGGACTCTGTTTCCTCTGTTTCTTGTGACAGAACTGGAAAATCTTCTGTGTAGAATTGAATTATCATGTTTGGATTGTTTTGTGATCAGAATTGAGTGTACTTTGCAGGCTATGGTGGGAGAAATTTCAGTAGGTTGAGTGGGAATAGTAATGGAGGAGAAGTTAGTCCACCATCAAACAGATTGAACAACTCTCAAATTAGCTTCTCCTCTTTGCTACCTTCTTCTCTGGGGATGCTTTCTCAGATATCTGAACAAGTTGGGAGTGAAAAGCTTGCTAATACCAATGGGGAGGCTCAGTTCTTCGGCTCCTCCGGGTTCCCGTTCGCTTCCTGGAATGAATCGTCTCAGTTCTCCGAAGCTTTCCCCTGCGTCAAACGCGATCCCGATCCCGAAAGTAACAGGAAGTTGTTTTCTAGTAATCATCAGGTATATATGGATCAATTACTTAGCTGAGAAGTTCAAAAGCAGTATTTTGTTTTGTGCATATATATTGATGTTAACATGAGGAAAATTTGGTGTTGATCAGAACGGCGAGATCAGTAACCGAGTTCATTTGTTATCGCACCACTTGAGCTTGCCCAAGAACGCGTCCGACATAGCTTCCATCGAGAAGTTGTTGCAGCTCCAAGATTGCGTGCCTTGTCGAATTCGAGCGAAACGAGGCTGCGCAACTCATCCTCGCAGCATTGCAGAACGGGTAAGAGAATAATTAATTATCGAGTCAACAAGCTATCGACTTTTTCTCAATCACCAGCTAGAAATCTTTAAATCACACTGCAAATTACTAAAATTAGAGCCAATTTATTCGCAGGTGCGACGAACCCGAATCAGCGAGCGTATGAGGAAGCTACAAGAACTTGTACCAAACATGGACAAGGTATAAATACATACAGAAACTTCCCCACATCCAATCCACATATTAATCTCACTACAAAACTTCAAAACCCCATTTCCACTCTGACGTTTTTCTTCGTGTAGCAAACGAACACAGCGGACATGTTGGACTTAGCGGTCGAGTACATCAAAGAGCTTCAGAAACAGTTCAAGGTACAAAGAAACTAAACGATTCAGACTCCGAAAGATATATCTATAAATCCAACAGTTTTACAGAGGATTATCATACATATGTTTATTCGTTTGTTTTGCAGACGCTAAGTGACAACCGAACGAAATGCGTGTGTTTGAATATGCAGAAGCCGGTATCGAATCAGATAATG

mRNA sequence

ATGGGTTCGAATACCCACCAGAGTTTTCAGCAACCCAATTCGGCGTTGCTCCGATTTCGGTCTGCTCCCAGTTCTCTGTTCGCTGATTTCGCTCATGGGGTTGATTCGAAGCGGTTGAATCACTTCGAGGGCTCGGAATCCGAGCGATTTGTTTCCAGATTTCGCGGCGGCGGTGGCGGTGGAAATAGTAATGACTCCGAATCTACGGCGGCCGGAAACTACTCCTCTGCCCTGCCGCCGCATTACCCCCGCCACTCCTCCGCCGTGAGTTCTTCTTCTTGTTCCTCGTCTTCGGCTTCTTCTGCTACAACTTCTTCTTCTTCTTCTTCAATGAGTTGCTCTTATGGGTTGCTGGGTTCTAATCTTGCTCGCCAGAATAGCTCTCCTCCTGGAGTTTTCTCTCACCTCAATCAAAACGGCTATGGTGGGAGAAATTTCAGTAGGTTGAGTGGGAATAGTAATGGAGGAGAAGTTAGTCCACCATCAAACAGATTGAACAACTCTCAAATTAGCTTCTCCTCTTTGCTACCTTCTTCTCTGGGGATGCTTTCTCAGATATCTGAACAAGTTGGGAGTGAAAAGCTTGCTAATACCAATGGGGAGGCTCAGTTCTTCGGCTCCTCCGGGTTCCCGTTCGCTTCCTGGAATGAATCGTCTCAGTTCTCCGAAGCTTTCCCCTGCGTCAAACGCGATCCCGATCCCGAAAGTAACAGGAAGTTGTTTTCTAGTAATCATCAGAACGGCGAGATCAGTAACCGAGTTCATTTGTTATCGCACCACTTGAGCTTGCCCAAGAACGCGTCCGACATAGCTTCCATCGAGAAGTTGTTGCAGCTCCAAGATTGCGTGCCTTGTCGAATTCGAGCGAAACGAGGCTGCGCAACTCATCCTCGCAGCATTGCAGAACGGGTGCGACGAACCCGAATCAGCGAGCGTATGAGGAAGCTACAAGAACTTGTACCAAACATGGACAAGCAAACGAACACAGCGGACATGTTGGACTTAGCGGTCGAGTACATCAAAGAGCTTCAGAAACAGTTCAAGACGCTAAGTGACAACCGAACGAAATGCGTGTGTTTGAATATGCAGAAGCCGGTATCGAATCAGATAATG

Coding sequence (CDS)

ATGGGTTCGAATACCCACCAGAGTTTTCAGCAACCCAATTCGGCGTTGCTCCGATTTCGGTCTGCTCCCAGTTCTCTGTTCGCTGATTTCGCTCATGGGGTTGATTCGAAGCGGTTGAATCACTTCGAGGGCTCGGAATCCGAGCGATTTGTTTCCAGATTTCGCGGCGGCGGTGGCGGTGGAAATAGTAATGACTCCGAATCTACGGCGGCCGGAAACTACTCCTCTGCCCTGCCGCCGCATTACCCCCGCCACTCCTCCGCCGTGAGTTCTTCTTCTTGTTCCTCGTCTTCGGCTTCTTCTGCTACAACTTCTTCTTCTTCTTCTTCAATGAGTTGCTCTTATGGGTTGCTGGGTTCTAATCTTGCTCGCCAGAATAGCTCTCCTCCTGGAGTTTTCTCTCACCTCAATCAAAACGGCTATGGTGGGAGAAATTTCAGTAGGTTGAGTGGGAATAGTAATGGAGGAGAAGTTAGTCCACCATCAAACAGATTGAACAACTCTCAAATTAGCTTCTCCTCTTTGCTACCTTCTTCTCTGGGGATGCTTTCTCAGATATCTGAACAAGTTGGGAGTGAAAAGCTTGCTAATACCAATGGGGAGGCTCAGTTCTTCGGCTCCTCCGGGTTCCCGTTCGCTTCCTGGAATGAATCGTCTCAGTTCTCCGAAGCTTTCCCCTGCGTCAAACGCGATCCCGATCCCGAAAGTAACAGGAAGTTGTTTTCTAGTAATCATCAGAACGGCGAGATCAGTAACCGAGTTCATTTGTTATCGCACCACTTGAGCTTGCCCAAGAACGCGTCCGACATAGCTTCCATCGAGAAGTTGTTGCAGCTCCAAGATTGCGTGCCTTGTCGAATTCGAGCGAAACGAGGCTGCGCAACTCATCCTCGCAGCATTGCAGAACGGGTGCGACGAACCCGAATCAGCGAGCGTATGAGGAAGCTACAAGAACTTGTACCAAACATGGACAAGCAAACGAACACAGCGGACATGTTGGACTTAGCGGTCGAGTACATCAAAGAGCTTCAGAAACAGTTCAAGACGCTAAGTGACAACCGAACGAAATGCGTGTGTTTGAATATGCAGAAGCCGGTATCGAATCAGATAATG

Protein sequence

MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGGGNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGSNLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGEVSPPSNRLNNSQISFSSLLPSSLGMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPESNRKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKCVCLNMQKPVSNQIM
Homology
BLAST of MS023025 vs. NCBI nr
Match: XP_022154225.1 (transcription factor bHLH130 [Momordica charantia])

HSP 1 Score: 685.3 bits (1767), Expect = 3.0e-193
Identity = 366/371 (98.65%), Postives = 368/371 (99.19%), Query Frame = 0

Query: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60
           MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG
Sbjct: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60

Query: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS 120
           GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS
Sbjct: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS 120

Query: 121 NLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGEVSPPSNRLNNSQISFSSLLPSSL 180
           NLARQNSSPPGVFSHLNQNGYGGRNFSRLSG+SNGGEVSPPSNRLNNSQISFSSLLPSSL
Sbjct: 121 NLARQNSSPPGVFSHLNQNGYGGRNFSRLSGSSNGGEVSPPSNRLNNSQISFSSLLPSSL 180

Query: 181 GMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPESNRKL 240
           GMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFS+AFPCVK DPDPESNRKL
Sbjct: 181 GMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFSQAFPCVKCDPDPESNRKL 240

Query: 241 FSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATHPRSI 300
           FSSNHQNGEISNRVHLLSHHLS PKNASDIASIEKLLQLQD VPCRIRAKRGCATHPRSI
Sbjct: 241 FSSNHQNGEISNRVHLLSHHLSFPKNASDIASIEKLLQLQDSVPCRIRAKRGCATHPRSI 300

Query: 301 AERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKCVCL 360
           AERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKCVCL
Sbjct: 301 AERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKCVCL 360

Query: 361 NMQKPVSNQIM 372
           NMQKPVSNQIM
Sbjct: 361 NMQKPVSNQIM 371

BLAST of MS023025 vs. NCBI nr
Match: XP_038906316.1 (transcription factor bHLH130 [Benincasa hispida])

HSP 1 Score: 542.0 bits (1395), Expect = 4.1e-150
Identity = 308/373 (82.57%), Postives = 326/373 (87.40%), Query Frame = 0

Query: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60
           MGSNTHQSFQQPNSALLRFRSAPSSLFADFA G+DSKR N FEGSESER VSRF   GGG
Sbjct: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAQGIDSKRSNPFEGSESERLVSRFGSRGGG 60

Query: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS 120
           GNSNDSES  AGNY+S LPPHYPR +SAV   +CSSSS+SS   SSSSSSM  S G LG 
Sbjct: 61  GNSNDSESPVAGNYTSGLPPHYPRLNSAV---NCSSSSSSSC--SSSSSSMCSSLGFLGP 120

Query: 121 NLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGEVSPPSNRLNNSQISFSSLLPSSL 180
           NL RQ+SSP GVFS LNQNG+GG NFSRLSGN+NGGEVSP SNRL  SQISFSSLLPSSL
Sbjct: 121 NLVRQSSSPAGVFSQLNQNGFGGGNFSRLSGNNNGGEVSPSSNRL-TSQISFSSLLPSSL 180

Query: 181 GMLSQISEQ-VGSEKLANT-NGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPESNR 240
           GM  QISEQ VG+EKLAN+ NGE QFF  SGFPFASWNESSQF E F  +KRDPD  SN+
Sbjct: 181 GMFPQISEQVVGNEKLANSNNGETQFFTPSGFPFASWNESSQFPETFTSIKRDPD--SNK 240

Query: 241 KLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATHPR 300
           KLFSS+ QNGEI NRVHLLSHHLSLPKNA+D+ASIEKLLQLQD VPCRIRAKRGCATHPR
Sbjct: 241 KLFSSSPQNGEIGNRVHLLSHHLSLPKNATDVASIEKLLQLQDAVPCRIRAKRGCATHPR 300

Query: 301 SIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKCV 360
           SIAERVRRTRISERMRKLQ+LVPNMDKQTNTADMLDLAV+YIKELQKQFKTLSDNR  CV
Sbjct: 301 SIAERVRRTRISERMRKLQDLVPNMDKQTNTADMLDLAVDYIKELQKQFKTLSDNRANCV 360

Query: 361 CLNMQKPVSNQIM 372
           CLNMQKPVS+QIM
Sbjct: 361 CLNMQKPVSSQIM 365

BLAST of MS023025 vs. NCBI nr
Match: XP_008443086.1 (PREDICTED: transcription factor bHLH130 [Cucumis melo] >KAA0043711.1 transcription factor bHLH130 [Cucumis melo var. makuwa] >TYK25423.1 transcription factor bHLH130 [Cucumis melo var. makuwa])

HSP 1 Score: 539.7 bits (1389), Expect = 2.0e-149
Identity = 310/374 (82.89%), Postives = 330/374 (88.24%), Query Frame = 0

Query: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60
           MG+NTHQSFQQPNSALLRFRSAPSSLFADFAHG+DSKRLN FE SESER VSRF   GGG
Sbjct: 1   MGTNTHQSFQQPNSALLRFRSAPSSLFADFAHGIDSKRLNPFESSESERLVSRFGSRGGG 60

Query: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS 120
            NSNDSES  AGNYSS LPPHYPR SSAV+ SS SSSS+SS++ SSSSSSM  S G LGS
Sbjct: 61  CNSNDSESPVAGNYSSGLPPHYPRLSSAVNCSS-SSSSSSSSSCSSSSSSMCSSLGFLGS 120

Query: 121 NLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGE-VSPPSNRLNNSQISFSSLLPSS 180
           NL RQ+SSP GV S  NQNGYGG +FSRLSGN+NGGE VSP SNRL NSQISFSSLLPSS
Sbjct: 121 NLVRQSSSPAGVLSQFNQNGYGGGSFSRLSGNNNGGEVVSPSSNRL-NSQISFSSLLPSS 180

Query: 181 LGMLSQISEQ-VGSEKLANT-NGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPESN 240
           LGM  QISEQ VG+EKL+N+ NGE Q+F  SGFPFASWNESSQFSE FP VKRDPD  SN
Sbjct: 181 LGMFPQISEQVVGNEKLSNSNNGETQYFTPSGFPFASWNESSQFSETFPGVKRDPD--SN 240

Query: 241 RKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATHP 300
           +K FSS+HQNGEI NRVHLLSHHLSLPKN SD+ASIEKLLQLQD VPCRIRAKRGCATHP
Sbjct: 241 KK-FSSSHQNGEIGNRVHLLSHHLSLPKNVSDVASIEKLLQLQDAVPCRIRAKRGCATHP 300

Query: 301 RSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKC 360
           RSIAERVRRTRISERMRKLQ+LVPNMDKQTNTADMLDLAV+YIKELQKQFKTLSDNR  C
Sbjct: 301 RSIAERVRRTRISERMRKLQDLVPNMDKQTNTADMLDLAVDYIKELQKQFKTLSDNRANC 360

Query: 361 VCLNMQKPVSNQIM 372
           VC+NMQKP+SNQIM
Sbjct: 361 VCVNMQKPLSNQIM 369

BLAST of MS023025 vs. NCBI nr
Match: XP_031737624.1 (transcription factor bHLH130 [Cucumis sativus] >KGN59326.1 hypothetical protein Csa_002059 [Cucumis sativus])

HSP 1 Score: 530.8 bits (1366), Expect = 9.5e-147
Identity = 306/374 (81.82%), Postives = 326/374 (87.17%), Query Frame = 0

Query: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60
           MG+NTHQSFQQPNSALLRFRSAPSSLFADF+HG+DSKRLN FEGSESER VSRF     G
Sbjct: 1   MGTNTHQSFQQPNSALLRFRSAPSSLFADFSHGIDSKRLNPFEGSESERLVSRFGSRADG 60

Query: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS 120
            NSNDSES  AGNYSS LPPHYPR SSAV   +CSSSS SS++ SSSSSSM  S G LGS
Sbjct: 61  CNSNDSESPVAGNYSSGLPPHYPRLSSAV---NCSSSS-SSSSCSSSSSSMCSSLGFLGS 120

Query: 121 NLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGE-VSPPSNRLNNSQISFSSLLPSS 180
           NL RQ+SSP GV S LNQNGYGG +FSRLSGN+NG E VSP SNRL NSQISFSSL+PSS
Sbjct: 121 NLVRQSSSPAGVLSQLNQNGYGGGSFSRLSGNNNGVEVVSPSSNRL-NSQISFSSLVPSS 180

Query: 181 LGMLSQISEQ-VGSEKLANT-NGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPESN 240
           LGM  QISEQ VG+EKL+N+ NGE QFF  SGFPFASWNESSQFSE FP +KRDPD  SN
Sbjct: 181 LGMFPQISEQVVGNEKLSNSNNGETQFFTPSGFPFASWNESSQFSETFPGIKRDPD--SN 240

Query: 241 RKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATHP 300
           +K FSS HQNGEI NRVHLLSHHLSLPKN SD+ASIEKLLQLQD VPCRIRAKRGCATHP
Sbjct: 241 KK-FSSGHQNGEIGNRVHLLSHHLSLPKNVSDVASIEKLLQLQDAVPCRIRAKRGCATHP 300

Query: 301 RSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKC 360
           RSIAERVRRTRISERMRKLQ+LVPNMDKQTNTADMLDLAV+YIKELQKQFKTLSDNR  C
Sbjct: 301 RSIAERVRRTRISERMRKLQDLVPNMDKQTNTADMLDLAVDYIKELQKQFKTLSDNRANC 360

Query: 361 VCLNMQKPVSNQIM 372
           VC+NMQKP+SNQIM
Sbjct: 361 VCVNMQKPLSNQIM 366

BLAST of MS023025 vs. NCBI nr
Match: XP_022935700.1 (transcription factor bHLH130-like [Cucurbita moschata])

HSP 1 Score: 508.1 bits (1307), Expect = 6.6e-140
Identity = 290/375 (77.33%), Postives = 315/375 (84.00%), Query Frame = 0

Query: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60
           MGSNTH SFQQPNSALLRFRSAPSSL AD+A G+DSKR N FE SESER VSRF      
Sbjct: 1   MGSNTHHSFQQPNSALLRFRSAPSSLLADYAQGIDSKRSNPFESSESERMVSRF------ 60

Query: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAV---SSSSCSSSSASSATTSSSSSSMSCSYGL 120
           G+ NDSES AAGNY S LPPHYPR S+AV   SSSS SSSS+SS+++SSSSSSMS S G 
Sbjct: 61  GSRNDSESPAAGNYCSGLPPHYPRPSTAVNCSSSSSSSSSSSSSSSSSSSSSSMSSSVGF 120

Query: 121 LGSNLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGEVSPPSNRLNNSQISFSSLLP 180
           LGSNL RQ+SSP GVFS LNQNGYGG +F+RLSG++N G            ++SFSSLLP
Sbjct: 121 LGSNLVRQSSSPAGVFSQLNQNGYGGGSFNRLSGSNNNG----------GGELSFSSLLP 180

Query: 181 SSLGMLSQISEQV-GSEKLANTNGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPES 240
           SSLGMLS ISEQV G+EKL NTNGE QFF  +GFPFASWNESSQFS+ FP VK DPD  S
Sbjct: 181 SSLGMLSHISEQVIGNEKLTNTNGEPQFFTPTGFPFASWNESSQFSDTFPVVKHDPD--S 240

Query: 241 NRKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATH 300
           NRKLFSS+HQNGEI NRVHLLSHHLSLPKN SDIASIEKLLQLQD VPCRIRAKRGCATH
Sbjct: 241 NRKLFSSSHQNGEIGNRVHLLSHHLSLPKNTSDIASIEKLLQLQDAVPCRIRAKRGCATH 300

Query: 301 PRSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTK 360
           PRSIAERVRRTRISERMRKLQ+LVPNMDKQTNTADMLDLAV+YIKELQ+QFKTLSDNR  
Sbjct: 301 PRSIAERVRRTRISERMRKLQDLVPNMDKQTNTADMLDLAVDYIKELQRQFKTLSDNRAN 357

Query: 361 CVCLNMQKPVSNQIM 372
           CVC+N QK VSNQ++
Sbjct: 361 CVCVNTQKAVSNQMI 357

BLAST of MS023025 vs. ExPASy Swiss-Prot
Match: Q66GR3 (Transcription factor bHLH130 OS=Arabidopsis thaliana OX=3702 GN=BHLH130 PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.4e-60
Identity = 182/394 (46.19%), Postives = 223/394 (56.60%), Query Frame = 0

Query: 1   MGSNTHQSFQQP-NSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRF----- 60
           M SN H     P  S LLRFRSAPSS+ A F   VD  ++    G +S+R +SRF     
Sbjct: 1   MDSNNHLYDPNPTGSGLLRFRSAPSSVLAAF---VDDDKI----GFDSDRLLSRFVTSNG 60

Query: 61  -RGGGGGGNSNDSESTAAGNYSSA------------------LPPHYPRHSSAVSSSSCS 120
             G  G     D    +  N S +                  LPPHYPR S  + +S   
Sbjct: 61  VNGDLGSPKFEDKSPVSLTNTSVSYAATLPPPPQLEPSSFLGLPPHYPRQSKGIMNSVGL 120

Query: 121 SSSASSATTSSSSSSMSCSYGLLGSNLARQNSSPPGVFSHL-NQNGYGG-RNFSRLSGNS 180
                     +           + SNL RQ+SSP G+F++L +QNGYG  RN      N 
Sbjct: 121 DQFLGINNHHTKP---------VESNLLRQSSSPAGMFTNLSDQNGYGSMRNLM----NY 180

Query: 181 NGGEVSPPSNRLNNSQISFSSLLPSSLGMLSQISEQVGSEKLANTNGEAQFFGSSGFPFA 240
              E SP ++       S SS  PSSLGMLSQI E                   + FP++
Sbjct: 181 EEDEESPSNSNGLRRHCSLSSRPPSSLGMLSQIPE---------------IAPETNFPYS 240

Query: 241 SWNESSQFSEAFPCVKRDPDPESNRKLFSSNHQNGEISNRVHLLSHHLSLPKN---ASDI 300
            WN+ S F +    +KR  + E + KLF    QNGE  NR+ LLSHHLSLPK+   ASD+
Sbjct: 241 HWNDPSSFIDNLSSLKR--EAEDDGKLF-LGAQNGESGNRMQLLSHHLSLPKSSSTASDM 300

Query: 301 ASIEKLLQLQDCVPCRIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTA 360
            S++K LQLQD VPC+IRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNT+
Sbjct: 301 VSVDKYLQLQDSVPCKIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTS 356

Query: 361 DMLDLAVEYIKELQKQFKTLSDNRTKCVCLNMQK 365
           DMLDLAV+YIK+LQ+Q+K L+DNR  C C+N +K
Sbjct: 361 DMLDLAVDYIKDLQRQYKILNDNRANCKCMNKEK 356

BLAST of MS023025 vs. ExPASy Swiss-Prot
Match: Q9C690 (Transcription factor bHLH122 OS=Arabidopsis thaliana OX=3702 GN=BHLH122 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 7.8e-35
Identity = 138/377 (36.60%), Postives = 191/377 (50.66%), Query Frame = 0

Query: 6   HQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGGGNSND 65
           HQ  +  NS L+R++SAPSS F+ F   ++ + L+     E+ER +S F       ++ D
Sbjct: 15  HQHQRPRNSGLIRYQSAPSSYFSSFGESIE-EFLDRPTSPETERILSGFLQTTDTSDNVD 74

Query: 66  S--ESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSS---SSSMSCSYGLLGS 125
           S    T   + +   PP              ++++A     S     S +   S G + S
Sbjct: 75  SFLHHTFNSDGTEKKPPEVKTEDEDAEIPVTATATAMEVVVSGDGEISVNPEVSIGYVAS 134

Query: 126 -----------------NLARQNSSPPGVFSHLN-QNGYGGRNFSRLSGNSNGGEVSPPS 185
                            NLAR NSSP G+FS ++ +  Y      +  G   G  V   S
Sbjct: 135 VSRNKRPREKDDRTPVNNLARHNSSPAGLFSSIDVETAYAA--VMKSMGGFGGSNVMSTS 194

Query: 186 NRLNNSQISFSSLLPSSLGMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFS 245
           N   +S    S LLP +   +S ISE       ++         S GF  +  NE S  S
Sbjct: 195 NTEASSLTPRSKLLPPTSRAMSPISEVDVKPGFSSRLPPRTL--SGGFNRSFGNEGSASS 254

Query: 246 EAFPCVKRDPDPESNRKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDC 305
           +     +         K      ++ + ++R   L+HH+SLPK+ SDI  +     L D 
Sbjct: 255 KLTALARTQSGGLDQYKT-----KDEDSASRRPPLAHHMSLPKSLSDIEQL-----LSDS 314

Query: 306 VPCRIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKE 360
           +PC+IRAKRGCATHPRSIAERVRRT+ISERMRKLQ+LVPNMD QTNTADMLDLAV+YIK+
Sbjct: 315 IPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQDLVPNMDTQTNTADMLDLAVQYIKD 374

BLAST of MS023025 vs. ExPASy Swiss-Prot
Match: Q9C8P8 (Transcription factor bHLH80 OS=Arabidopsis thaliana OX=3702 GN=BHLH80 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.7e-27
Identity = 59/80 (73.75%), Postives = 71/80 (88.75%), Query Frame = 0

Query: 280 QDCVPCRIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEY 339
           +D VPCR+RAKRGCATHPRSIAERVRRTRIS+R+R+LQELVPNMDKQTNTADML+ AVEY
Sbjct: 174 EDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRRLQELVPNMDKQTNTADMLEEAVEY 233

Query: 340 IKELQKQFKTLSDNRTKCVC 360
           +K LQ Q + L++ + +C C
Sbjct: 234 VKALQSQIQELTEQQKRCKC 253

BLAST of MS023025 vs. ExPASy Swiss-Prot
Match: Q9M0R0 (Transcription factor bHLH81 OS=Arabidopsis thaliana OX=3702 GN=BHLH81 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.5e-27
Identity = 85/203 (41.87%), Postives = 121/203 (59.61%), Query Frame = 0

Query: 162 SNRLNNSQISFSSLLPSSLGMLSQ----ISEQVGSEKLANTNGEAQFFGSSGFPFASWNE 221
           SN L  S+ SF   +P   G+  Q          ++ L+ ++G  Q FG      A+++ 
Sbjct: 66  SNDLPTSRGSFEFPIPVEQGLYQQGGFHRQNSTPADFLSGSDGFIQSFGIQ----ANYDY 125

Query: 222 SSQFSEAFPCVKRDPDPESNRKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLL 281
            S   +  P  KR  + E+   LFSS     E ++++        +P   S ++ +    
Sbjct: 126 LSGNIDVSPGSKRSREMEA---LFSS----PEFTSQMKGEQSSGQVPTGVSSMSDMNMEN 185

Query: 282 QLQDCVPCRIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAV 341
            ++D V  R+RAKRGCATHPRSIAERVRRTRIS+R+RKLQELVPNMDKQTNTADML+ AV
Sbjct: 186 LMEDSVAFRVRAKRGCATHPRSIAERVRRTRISDRIRKLQELVPNMDKQTNTADMLEEAV 245

Query: 342 EYIKELQKQFKTLSDNRTKCVCL 361
           EY+K LQ+Q + L++ + +C C+
Sbjct: 246 EYVKVLQRQIQELTEEQKRCTCI 257

BLAST of MS023025 vs. ExPASy Swiss-Prot
Match: Q8H102 (Transcription factor bHLH128 OS=Arabidopsis thaliana OX=3702 GN=BHLH128 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 7.6e-22
Identity = 130/382 (34.03%), Postives = 186/382 (48.69%), Query Frame = 0

Query: 3   SNTHQSFQQPNSALLRFRSAPSSLF---ADFAHGVDSKRLNHFEGSE--SERFVSRFRGG 62
           S++ +S       L+R+ SAP S      D   G  S     F G +  S+ F+  F  G
Sbjct: 10  SSSQRSSLPGGGGLIRYGSAPGSFLNSVVDEVIGGGSSNARDFTGYQPSSDNFIGNFFTG 69

Query: 63  GGGGNSNDSESTAAG-NYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYG 122
               +S  S+ST  G N SS        +++  S+       +       S    S   G
Sbjct: 70  AADSSSLRSDSTTCGVNNSSDGQKQLGNNNNNNSNKDIFLDRSYGGFNEISQQHKSNDIG 129

Query: 123 LLGS----NLARQNSSPPGVFSHLNQNGYGGRNFS--------RLSGNSNGGEVSPPSNR 182
              S    +LARQ SSP   F++L  +     NFS           G SNGG      +R
Sbjct: 130 GGNSSGSYSLARQRSSPADFFTYLASD---KNNFSLNQPTSDYSPQGGSNGGR---GHSR 189

Query: 183 LNNSQISFSSLLPSSLGMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFSEA 242
           L  SQ+SF++    SL  +++++E    +   ++   A F  ++   +   + S  F+  
Sbjct: 190 L-KSQLSFTN--HDSLARINEVNETPVHDGSGHSFSAASFGAATTDSWDDGSGSIGFTVT 249

Query: 243 FPCVKRDPDPESNRKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQL-QDCV 302
            P  KR  D +S   LFS                   SLP + S +  ++  +QL +D V
Sbjct: 250 RPS-KRSKDMDSG--LFS-----------------QYSLPSDTS-MNYMDNFMQLPEDSV 309

Query: 303 PCRIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKEL 362
           PC+IRAKRGCATHPRSIAER RRTRIS +++KLQ+LVPNMDKQT+ +DMLDLAV++IK L
Sbjct: 310 PCKIRAKRGCATHPRSIAERERRTRISGKLKKLQDLVPNMDKQTSYSDMLDLAVQHIKGL 361

Query: 363 QKQFKTLSDNRTKCVCLNMQKP 366
           Q Q + L  ++  C C   +KP
Sbjct: 370 QHQLQNLKKDQENCTCGCSEKP 361

BLAST of MS023025 vs. ExPASy TrEMBL
Match: A0A6J1DJ13 (transcription factor bHLH130 OS=Momordica charantia OX=3673 GN=LOC111021535 PE=4 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.5e-193
Identity = 366/371 (98.65%), Postives = 368/371 (99.19%), Query Frame = 0

Query: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60
           MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG
Sbjct: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60

Query: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS 120
           GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS
Sbjct: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS 120

Query: 121 NLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGEVSPPSNRLNNSQISFSSLLPSSL 180
           NLARQNSSPPGVFSHLNQNGYGGRNFSRLSG+SNGGEVSPPSNRLNNSQISFSSLLPSSL
Sbjct: 121 NLARQNSSPPGVFSHLNQNGYGGRNFSRLSGSSNGGEVSPPSNRLNNSQISFSSLLPSSL 180

Query: 181 GMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPESNRKL 240
           GMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFS+AFPCVK DPDPESNRKL
Sbjct: 181 GMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFSQAFPCVKCDPDPESNRKL 240

Query: 241 FSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATHPRSI 300
           FSSNHQNGEISNRVHLLSHHLS PKNASDIASIEKLLQLQD VPCRIRAKRGCATHPRSI
Sbjct: 241 FSSNHQNGEISNRVHLLSHHLSFPKNASDIASIEKLLQLQDSVPCRIRAKRGCATHPRSI 300

Query: 301 AERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKCVCL 360
           AERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKCVCL
Sbjct: 301 AERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKCVCL 360

Query: 361 NMQKPVSNQIM 372
           NMQKPVSNQIM
Sbjct: 361 NMQKPVSNQIM 371

BLAST of MS023025 vs. ExPASy TrEMBL
Match: A0A5D3DP51 (Transcription factor bHLH130 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005660 PE=4 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 9.9e-150
Identity = 310/374 (82.89%), Postives = 330/374 (88.24%), Query Frame = 0

Query: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60
           MG+NTHQSFQQPNSALLRFRSAPSSLFADFAHG+DSKRLN FE SESER VSRF   GGG
Sbjct: 1   MGTNTHQSFQQPNSALLRFRSAPSSLFADFAHGIDSKRLNPFESSESERLVSRFGSRGGG 60

Query: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS 120
            NSNDSES  AGNYSS LPPHYPR SSAV+ SS SSSS+SS++ SSSSSSM  S G LGS
Sbjct: 61  CNSNDSESPVAGNYSSGLPPHYPRLSSAVNCSS-SSSSSSSSSCSSSSSSMCSSLGFLGS 120

Query: 121 NLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGE-VSPPSNRLNNSQISFSSLLPSS 180
           NL RQ+SSP GV S  NQNGYGG +FSRLSGN+NGGE VSP SNRL NSQISFSSLLPSS
Sbjct: 121 NLVRQSSSPAGVLSQFNQNGYGGGSFSRLSGNNNGGEVVSPSSNRL-NSQISFSSLLPSS 180

Query: 181 LGMLSQISEQ-VGSEKLANT-NGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPESN 240
           LGM  QISEQ VG+EKL+N+ NGE Q+F  SGFPFASWNESSQFSE FP VKRDPD  SN
Sbjct: 181 LGMFPQISEQVVGNEKLSNSNNGETQYFTPSGFPFASWNESSQFSETFPGVKRDPD--SN 240

Query: 241 RKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATHP 300
           +K FSS+HQNGEI NRVHLLSHHLSLPKN SD+ASIEKLLQLQD VPCRIRAKRGCATHP
Sbjct: 241 KK-FSSSHQNGEIGNRVHLLSHHLSLPKNVSDVASIEKLLQLQDAVPCRIRAKRGCATHP 300

Query: 301 RSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKC 360
           RSIAERVRRTRISERMRKLQ+LVPNMDKQTNTADMLDLAV+YIKELQKQFKTLSDNR  C
Sbjct: 301 RSIAERVRRTRISERMRKLQDLVPNMDKQTNTADMLDLAVDYIKELQKQFKTLSDNRANC 360

Query: 361 VCLNMQKPVSNQIM 372
           VC+NMQKP+SNQIM
Sbjct: 361 VCVNMQKPLSNQIM 369

BLAST of MS023025 vs. ExPASy TrEMBL
Match: A0A1S3B7A4 (transcription factor bHLH130 OS=Cucumis melo OX=3656 GN=LOC103486790 PE=4 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 9.9e-150
Identity = 310/374 (82.89%), Postives = 330/374 (88.24%), Query Frame = 0

Query: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60
           MG+NTHQSFQQPNSALLRFRSAPSSLFADFAHG+DSKRLN FE SESER VSRF   GGG
Sbjct: 1   MGTNTHQSFQQPNSALLRFRSAPSSLFADFAHGIDSKRLNPFESSESERLVSRFGSRGGG 60

Query: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS 120
            NSNDSES  AGNYSS LPPHYPR SSAV+ SS SSSS+SS++ SSSSSSM  S G LGS
Sbjct: 61  CNSNDSESPVAGNYSSGLPPHYPRLSSAVNCSS-SSSSSSSSSCSSSSSSMCSSLGFLGS 120

Query: 121 NLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGE-VSPPSNRLNNSQISFSSLLPSS 180
           NL RQ+SSP GV S  NQNGYGG +FSRLSGN+NGGE VSP SNRL NSQISFSSLLPSS
Sbjct: 121 NLVRQSSSPAGVLSQFNQNGYGGGSFSRLSGNNNGGEVVSPSSNRL-NSQISFSSLLPSS 180

Query: 181 LGMLSQISEQ-VGSEKLANT-NGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPESN 240
           LGM  QISEQ VG+EKL+N+ NGE Q+F  SGFPFASWNESSQFSE FP VKRDPD  SN
Sbjct: 181 LGMFPQISEQVVGNEKLSNSNNGETQYFTPSGFPFASWNESSQFSETFPGVKRDPD--SN 240

Query: 241 RKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATHP 300
           +K FSS+HQNGEI NRVHLLSHHLSLPKN SD+ASIEKLLQLQD VPCRIRAKRGCATHP
Sbjct: 241 KK-FSSSHQNGEIGNRVHLLSHHLSLPKNVSDVASIEKLLQLQDAVPCRIRAKRGCATHP 300

Query: 301 RSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKC 360
           RSIAERVRRTRISERMRKLQ+LVPNMDKQTNTADMLDLAV+YIKELQKQFKTLSDNR  C
Sbjct: 301 RSIAERVRRTRISERMRKLQDLVPNMDKQTNTADMLDLAVDYIKELQKQFKTLSDNRANC 360

Query: 361 VCLNMQKPVSNQIM 372
           VC+NMQKP+SNQIM
Sbjct: 361 VCVNMQKPLSNQIM 369

BLAST of MS023025 vs. ExPASy TrEMBL
Match: A0A0A0LH01 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G810510 PE=4 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 4.6e-147
Identity = 306/374 (81.82%), Postives = 326/374 (87.17%), Query Frame = 0

Query: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60
           MG+NTHQSFQQPNSALLRFRSAPSSLFADF+HG+DSKRLN FEGSESER VSRF     G
Sbjct: 1   MGTNTHQSFQQPNSALLRFRSAPSSLFADFSHGIDSKRLNPFEGSESERLVSRFGSRADG 60

Query: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYGLLGS 120
            NSNDSES  AGNYSS LPPHYPR SSAV   +CSSSS SS++ SSSSSSM  S G LGS
Sbjct: 61  CNSNDSESPVAGNYSSGLPPHYPRLSSAV---NCSSSS-SSSSCSSSSSSMCSSLGFLGS 120

Query: 121 NLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGE-VSPPSNRLNNSQISFSSLLPSS 180
           NL RQ+SSP GV S LNQNGYGG +FSRLSGN+NG E VSP SNRL NSQISFSSL+PSS
Sbjct: 121 NLVRQSSSPAGVLSQLNQNGYGGGSFSRLSGNNNGVEVVSPSSNRL-NSQISFSSLVPSS 180

Query: 181 LGMLSQISEQ-VGSEKLANT-NGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPESN 240
           LGM  QISEQ VG+EKL+N+ NGE QFF  SGFPFASWNESSQFSE FP +KRDPD  SN
Sbjct: 181 LGMFPQISEQVVGNEKLSNSNNGETQFFTPSGFPFASWNESSQFSETFPGIKRDPD--SN 240

Query: 241 RKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATHP 300
           +K FSS HQNGEI NRVHLLSHHLSLPKN SD+ASIEKLLQLQD VPCRIRAKRGCATHP
Sbjct: 241 KK-FSSGHQNGEIGNRVHLLSHHLSLPKNVSDVASIEKLLQLQDAVPCRIRAKRGCATHP 300

Query: 301 RSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTKC 360
           RSIAERVRRTRISERMRKLQ+LVPNMDKQTNTADMLDLAV+YIKELQKQFKTLSDNR  C
Sbjct: 301 RSIAERVRRTRISERMRKLQDLVPNMDKQTNTADMLDLAVDYIKELQKQFKTLSDNRANC 360

Query: 361 VCLNMQKPVSNQIM 372
           VC+NMQKP+SNQIM
Sbjct: 361 VCVNMQKPLSNQIM 366

BLAST of MS023025 vs. ExPASy TrEMBL
Match: A0A6J1FBE1 (transcription factor bHLH130-like OS=Cucurbita moschata OX=3662 GN=LOC111442479 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 3.2e-140
Identity = 290/375 (77.33%), Postives = 315/375 (84.00%), Query Frame = 0

Query: 1   MGSNTHQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGG 60
           MGSNTH SFQQPNSALLRFRSAPSSL AD+A G+DSKR N FE SESER VSRF      
Sbjct: 1   MGSNTHHSFQQPNSALLRFRSAPSSLLADYAQGIDSKRSNPFESSESERMVSRF------ 60

Query: 61  GNSNDSESTAAGNYSSALPPHYPRHSSAV---SSSSCSSSSASSATTSSSSSSMSCSYGL 120
           G+ NDSES AAGNY S LPPHYPR S+AV   SSSS SSSS+SS+++SSSSSSMS S G 
Sbjct: 61  GSRNDSESPAAGNYCSGLPPHYPRPSTAVNCSSSSSSSSSSSSSSSSSSSSSSMSSSVGF 120

Query: 121 LGSNLARQNSSPPGVFSHLNQNGYGGRNFSRLSGNSNGGEVSPPSNRLNNSQISFSSLLP 180
           LGSNL RQ+SSP GVFS LNQNGYGG +F+RLSG++N G            ++SFSSLLP
Sbjct: 121 LGSNLVRQSSSPAGVFSQLNQNGYGGGSFNRLSGSNNNG----------GGELSFSSLLP 180

Query: 181 SSLGMLSQISEQV-GSEKLANTNGEAQFFGSSGFPFASWNESSQFSEAFPCVKRDPDPES 240
           SSLGMLS ISEQV G+EKL NTNGE QFF  +GFPFASWNESSQFS+ FP VK DPD  S
Sbjct: 181 SSLGMLSHISEQVIGNEKLTNTNGEPQFFTPTGFPFASWNESSQFSDTFPVVKHDPD--S 240

Query: 241 NRKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDCVPCRIRAKRGCATH 300
           NRKLFSS+HQNGEI NRVHLLSHHLSLPKN SDIASIEKLLQLQD VPCRIRAKRGCATH
Sbjct: 241 NRKLFSSSHQNGEIGNRVHLLSHHLSLPKNTSDIASIEKLLQLQDAVPCRIRAKRGCATH 300

Query: 301 PRSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKELQKQFKTLSDNRTK 360
           PRSIAERVRRTRISERMRKLQ+LVPNMDKQTNTADMLDLAV+YIKELQ+QFKTLSDNR  
Sbjct: 301 PRSIAERVRRTRISERMRKLQDLVPNMDKQTNTADMLDLAVDYIKELQRQFKTLSDNRAN 357

Query: 361 CVCLNMQKPVSNQIM 372
           CVC+N QK VSNQ++
Sbjct: 361 CVCVNTQKAVSNQMI 357

BLAST of MS023025 vs. TAIR 10
Match: AT2G42280.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 235.0 bits (598), Expect = 1.0e-61
Identity = 182/394 (46.19%), Postives = 223/394 (56.60%), Query Frame = 0

Query: 1   MGSNTHQSFQQP-NSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRF----- 60
           M SN H     P  S LLRFRSAPSS+ A F   VD  ++    G +S+R +SRF     
Sbjct: 1   MDSNNHLYDPNPTGSGLLRFRSAPSSVLAAF---VDDDKI----GFDSDRLLSRFVTSNG 60

Query: 61  -RGGGGGGNSNDSESTAAGNYSSA------------------LPPHYPRHSSAVSSSSCS 120
             G  G     D    +  N S +                  LPPHYPR S  + +S   
Sbjct: 61  VNGDLGSPKFEDKSPVSLTNTSVSYAATLPPPPQLEPSSFLGLPPHYPRQSKGIMNSVGL 120

Query: 121 SSSASSATTSSSSSSMSCSYGLLGSNLARQNSSPPGVFSHL-NQNGYGG-RNFSRLSGNS 180
                     +           + SNL RQ+SSP G+F++L +QNGYG  RN      N 
Sbjct: 121 DQFLGINNHHTKP---------VESNLLRQSSSPAGMFTNLSDQNGYGSMRNLM----NY 180

Query: 181 NGGEVSPPSNRLNNSQISFSSLLPSSLGMLSQISEQVGSEKLANTNGEAQFFGSSGFPFA 240
              E SP ++       S SS  PSSLGMLSQI E                   + FP++
Sbjct: 181 EEDEESPSNSNGLRRHCSLSSRPPSSLGMLSQIPE---------------IAPETNFPYS 240

Query: 241 SWNESSQFSEAFPCVKRDPDPESNRKLFSSNHQNGEISNRVHLLSHHLSLPKN---ASDI 300
            WN+ S F +    +KR  + E + KLF    QNGE  NR+ LLSHHLSLPK+   ASD+
Sbjct: 241 HWNDPSSFIDNLSSLKR--EAEDDGKLF-LGAQNGESGNRMQLLSHHLSLPKSSSTASDM 300

Query: 301 ASIEKLLQLQDCVPCRIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTA 360
            S++K LQLQD VPC+IRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNT+
Sbjct: 301 VSVDKYLQLQDSVPCKIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTS 356

Query: 361 DMLDLAVEYIKELQKQFKTLSDNRTKCVCLNMQK 365
           DMLDLAV+YIK+LQ+Q+K L+DNR  C C+N +K
Sbjct: 361 DMLDLAVDYIKDLQRQYKILNDNRANCKCMNKEK 356

BLAST of MS023025 vs. TAIR 10
Match: AT1G51140.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 5.5e-36
Identity = 138/377 (36.60%), Postives = 191/377 (50.66%), Query Frame = 0

Query: 6   HQSFQQPNSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRFRGGGGGGNSND 65
           HQ  +  NS L+R++SAPSS F+ F   ++ + L+     E+ER +S F       ++ D
Sbjct: 15  HQHQRPRNSGLIRYQSAPSSYFSSFGESIE-EFLDRPTSPETERILSGFLQTTDTSDNVD 74

Query: 66  S--ESTAAGNYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSS---SSSMSCSYGLLGS 125
           S    T   + +   PP              ++++A     S     S +   S G + S
Sbjct: 75  SFLHHTFNSDGTEKKPPEVKTEDEDAEIPVTATATAMEVVVSGDGEISVNPEVSIGYVAS 134

Query: 126 -----------------NLARQNSSPPGVFSHLN-QNGYGGRNFSRLSGNSNGGEVSPPS 185
                            NLAR NSSP G+FS ++ +  Y      +  G   G  V   S
Sbjct: 135 VSRNKRPREKDDRTPVNNLARHNSSPAGLFSSIDVETAYAA--VMKSMGGFGGSNVMSTS 194

Query: 186 NRLNNSQISFSSLLPSSLGMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFS 245
           N   +S    S LLP +   +S ISE       ++         S GF  +  NE S  S
Sbjct: 195 NTEASSLTPRSKLLPPTSRAMSPISEVDVKPGFSSRLPPRTL--SGGFNRSFGNEGSASS 254

Query: 246 EAFPCVKRDPDPESNRKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQLQDC 305
           +     +         K      ++ + ++R   L+HH+SLPK+ SDI  +     L D 
Sbjct: 255 KLTALARTQSGGLDQYKT-----KDEDSASRRPPLAHHMSLPKSLSDIEQL-----LSDS 314

Query: 306 VPCRIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKE 360
           +PC+IRAKRGCATHPRSIAERVRRT+ISERMRKLQ+LVPNMD QTNTADMLDLAV+YIK+
Sbjct: 315 IPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQDLVPNMDTQTNTADMLDLAVQYIKD 374

BLAST of MS023025 vs. TAIR 10
Match: AT2G42280.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.5e-33
Identity = 135/334 (40.42%), Postives = 168/334 (50.30%), Query Frame = 0

Query: 1   MGSNTHQSFQQP-NSALLRFRSAPSSLFADFAHGVDSKRLNHFEGSESERFVSRF----- 60
           M SN H     P  S LLRFRSAPSS+ A F   VD  ++    G +S+R +SRF     
Sbjct: 1   MDSNNHLYDPNPTGSGLLRFRSAPSSVLAAF---VDDDKI----GFDSDRLLSRFVTSNG 60

Query: 61  -RGGGGGGNSNDSESTAAGNYSSA------------------LPPHYPRHSSAVSSSSCS 120
             G  G     D    +  N S +                  LPPHYPR S  + +S   
Sbjct: 61  VNGDLGSPKFEDKSPVSLTNTSVSYAATLPPPPQLEPSSFLGLPPHYPRQSKGIMNSVGL 120

Query: 121 SSSASSATTSSSSSSMSCSYGLLGSNLARQNSSPPGVFSHL-NQNGYGG-RNFSRLSGNS 180
                     +           + SNL RQ+SSP G+F++L +QNGYG  RN      N 
Sbjct: 121 DQFLGINNHHTKP---------VESNLLRQSSSPAGMFTNLSDQNGYGSMRNLM----NY 180

Query: 181 NGGEVSPPSNRLNNSQISFSSLLPSSLGMLSQISEQVGSEKLANTNGEAQFFGSSGFPFA 240
              E SP ++       S SS  PSSLGMLSQI E                   + FP++
Sbjct: 181 EEDEESPSNSNGLRRHCSLSSRPPSSLGMLSQIPE---------------IAPETNFPYS 240

Query: 241 SWNESSQFSEAFPCVKRDPDPESNRKLFSSNHQNGEISNRVHLLSHHLSLPKN---ASDI 300
            WN+ S F +    +KR  + E + KLF    QNGE  NR+ LLSHHLSLPK+   ASD+
Sbjct: 241 HWNDPSSFIDNLSSLKR--EAEDDGKLF-LGAQNGESGNRMQLLSHHLSLPKSSSTASDM 296

Query: 301 ASIEKLLQLQDCVPCRIRAKRGCATHPRSIAERV 305
            S++K LQLQD VPC+IRAKRGCATHPRSIAERV
Sbjct: 301 VSVDKYLQLQDSVPCKIRAKRGCATHPRSIAERV 296

BLAST of MS023025 vs. TAIR 10
Match: AT4G09180.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 124.0 bits (310), Expect = 2.5e-28
Identity = 85/203 (41.87%), Postives = 121/203 (59.61%), Query Frame = 0

Query: 162 SNRLNNSQISFSSLLPSSLGMLSQ----ISEQVGSEKLANTNGEAQFFGSSGFPFASWNE 221
           SN L  S+ SF   +P   G+  Q          ++ L+ ++G  Q FG      A+++ 
Sbjct: 66  SNDLPTSRGSFEFPIPVEQGLYQQGGFHRQNSTPADFLSGSDGFIQSFGIQ----ANYDY 125

Query: 222 SSQFSEAFPCVKRDPDPESNRKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLL 281
            S   +  P  KR  + E+   LFSS     E ++++        +P   S ++ +    
Sbjct: 126 LSGNIDVSPGSKRSREMEA---LFSS----PEFTSQMKGEQSSGQVPTGVSSMSDMNMEN 185

Query: 282 QLQDCVPCRIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAV 341
            ++D V  R+RAKRGCATHPRSIAERVRRTRIS+R+RKLQELVPNMDKQTNTADML+ AV
Sbjct: 186 LMEDSVAFRVRAKRGCATHPRSIAERVRRTRISDRIRKLQELVPNMDKQTNTADMLEEAV 245

Query: 342 EYIKELQKQFKTLSDNRTKCVCL 361
           EY+K LQ+Q + L++ + +C C+
Sbjct: 246 EYVKVLQRQIQELTEEQKRCTCI 257

BLAST of MS023025 vs. TAIR 10
Match: AT1G05805.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 5.4e-23
Identity = 130/382 (34.03%), Postives = 186/382 (48.69%), Query Frame = 0

Query: 3   SNTHQSFQQPNSALLRFRSAPSSLF---ADFAHGVDSKRLNHFEGSE--SERFVSRFRGG 62
           S++ +S       L+R+ SAP S      D   G  S     F G +  S+ F+  F  G
Sbjct: 10  SSSQRSSLPGGGGLIRYGSAPGSFLNSVVDEVIGGGSSNARDFTGYQPSSDNFIGNFFTG 69

Query: 63  GGGGNSNDSESTAAG-NYSSALPPHYPRHSSAVSSSSCSSSSASSATTSSSSSSMSCSYG 122
               +S  S+ST  G N SS        +++  S+       +       S    S   G
Sbjct: 70  AADSSSLRSDSTTCGVNNSSDGQKQLGNNNNNNSNKDIFLDRSYGGFNEISQQHKSNDIG 129

Query: 123 LLGS----NLARQNSSPPGVFSHLNQNGYGGRNFS--------RLSGNSNGGEVSPPSNR 182
              S    +LARQ SSP   F++L  +     NFS           G SNGG      +R
Sbjct: 130 GGNSSGSYSLARQRSSPADFFTYLASD---KNNFSLNQPTSDYSPQGGSNGGR---GHSR 189

Query: 183 LNNSQISFSSLLPSSLGMLSQISEQVGSEKLANTNGEAQFFGSSGFPFASWNESSQFSEA 242
           L  SQ+SF++    SL  +++++E    +   ++   A F  ++   +   + S  F+  
Sbjct: 190 L-KSQLSFTN--HDSLARINEVNETPVHDGSGHSFSAASFGAATTDSWDDGSGSIGFTVT 249

Query: 243 FPCVKRDPDPESNRKLFSSNHQNGEISNRVHLLSHHLSLPKNASDIASIEKLLQL-QDCV 302
            P  KR  D +S   LFS                   SLP + S +  ++  +QL +D V
Sbjct: 250 RPS-KRSKDMDSG--LFS-----------------QYSLPSDTS-MNYMDNFMQLPEDSV 309

Query: 303 PCRIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTADMLDLAVEYIKEL 362
           PC+IRAKRGCATHPRSIAER RRTRIS +++KLQ+LVPNMDKQT+ +DMLDLAV++IK L
Sbjct: 310 PCKIRAKRGCATHPRSIAERERRTRISGKLKKLQDLVPNMDKQTSYSDMLDLAVQHIKGL 361

Query: 363 QKQFKTLSDNRTKCVCLNMQKP 366
           Q Q + L  ++  C C   +KP
Sbjct: 370 QHQLQNLKKDQENCTCGCSEKP 361

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154225.13.0e-19398.65transcription factor bHLH130 [Momordica charantia][more]
XP_038906316.14.1e-15082.57transcription factor bHLH130 [Benincasa hispida][more]
XP_008443086.12.0e-14982.89PREDICTED: transcription factor bHLH130 [Cucumis melo] >KAA0043711.1 transcripti... [more]
XP_031737624.19.5e-14781.82transcription factor bHLH130 [Cucumis sativus] >KGN59326.1 hypothetical protein ... [more]
XP_022935700.16.6e-14077.33transcription factor bHLH130-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q66GR31.4e-6046.19Transcription factor bHLH130 OS=Arabidopsis thaliana OX=3702 GN=BHLH130 PE=1 SV=... [more]
Q9C6907.8e-3536.60Transcription factor bHLH122 OS=Arabidopsis thaliana OX=3702 GN=BHLH122 PE=1 SV=... [more]
Q9C8P82.7e-2773.75Transcription factor bHLH80 OS=Arabidopsis thaliana OX=3702 GN=BHLH80 PE=2 SV=1[more]
Q9M0R03.5e-2741.87Transcription factor bHLH81 OS=Arabidopsis thaliana OX=3702 GN=BHLH81 PE=2 SV=1[more]
Q8H1027.6e-2234.03Transcription factor bHLH128 OS=Arabidopsis thaliana OX=3702 GN=BHLH128 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1DJ131.5e-19398.65transcription factor bHLH130 OS=Momordica charantia OX=3673 GN=LOC111021535 PE=4... [more]
A0A5D3DP519.9e-15082.89Transcription factor bHLH130 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3B7A49.9e-15082.89transcription factor bHLH130 OS=Cucumis melo OX=3656 GN=LOC103486790 PE=4 SV=1[more]
A0A0A0LH014.6e-14781.82BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G810510 PE=4 S... [more]
A0A6J1FBE13.2e-14077.33transcription factor bHLH130-like OS=Cucurbita moschata OX=3662 GN=LOC111442479 ... [more]
Match NameE-valueIdentityDescription
AT2G42280.11.0e-6146.19basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G51140.15.5e-3636.60basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G42280.21.5e-3340.42basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT4G09180.12.5e-2841.87basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G05805.15.4e-2334.03basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 299..349
e-value: 9.3E-12
score: 55.1
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 299..344
e-value: 4.0E-10
score: 39.5
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 293..343
score: 15.192548
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 294..363
e-value: 3.5E-14
score: 54.6
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 296..354
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 144..166
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..75
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..110
NoneNo IPR availablePANTHERPTHR16223TRANSCRIPTION FACTOR BHLH83-RELATEDcoord: 8..363
NoneNo IPR availablePANTHERPTHR16223:SF236TRANSCRIPTION FACTOR BHLH130coord: 8..363
NoneNo IPR availableCDDcd11393bHLH_AtbHLH_likecoord: 298..351
e-value: 1.72421E-21
score: 84.5403

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS023025.1MS023025.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046983 protein dimerization activity