MS023004 (gene) Bitter gourd (TR) v1

Overview
NameMS023004
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionATP synthase subunit b, chloroplastic
Locationscaffold357: 471367 .. 471589 (-)
RNA-Seq ExpressionMS023004
SyntenyMS023004
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCATGAGGAGGCAATTGACAAACAACTAGAAAAAGCTCGGTCTCGTTCACGAAAAGTCGAGATGGATGCTGATCAATTTCAAGAGAAAGGACGATACTCTGAAATAGAACGAGAAAAAACGAATTTGATTAACTCATCAACTTCTAAAAATTTGCAACAATTAGAAAATTAAAAAAATGGAACTATTCGTTTTGAATAACACAGAGCGATTAATCAAGTAT

mRNA sequence

CTTCATGAGGAGGCAATTGACAAACAACTAGAAAAAGCTCGGTCTCGTTCACGAAAAGTCGAGATGGATGCTGATCAATTTCAAGAGAAAGGACGATACTCTGAAATAGAACGAGAAAAAACGAATTTGATTAACTCATCAACTTCTAAAAATTTGCAACAATTAGAAAATAAAAAAATGGAACTATTCGTTTTGAATAACACAGAGCGATTAATCAAGTAT

Coding sequence (CDS)

CTTCATGAGGAGGCAATTGACAAACAACTAGAAAAAGCTCGGTCTCGTTCACGAAAAGTCGAGATGGATGCTGATCAATTTCAAGAGAAAGGACGATACTCTGAAATAGAACGAGAAAAAACGAATTTGATTAACTCATCAACTTCTAAAAATTTGCAACAATTAGAAAATAAAAAAATGGAACTATTCGTTTTGAATAACACAGAGCGATTAATCAAGTAT

Protein sequence

LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKMELFVLNNTERLIKY
Homology
BLAST of MS023004 vs. NCBI nr
Match: CAA9893319.1 (unnamed protein product [Spirodela intermedia])

HSP 1 Score: 69.7 bits (169), Expect = 1.2e-08
Identity = 44/71 (61.97%), Postives = 56/71 (78.87%), Query Frame = 0

Query: 4   EAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKMELF 63
           +A  +QLEKAR+R RKVE +A++++  G YSEIEREK NLIN +TS NL++LEN K E  
Sbjct: 75  QAAIEQLEKARARLRKVETEANEYRVNG-YSEIEREKENLIN-ATSDNLERLENYKNETL 134

Query: 64  VLNNTERLIKY 75
           +LNN ERLIKY
Sbjct: 135 LLNNKERLIKY 143

BLAST of MS023004 vs. NCBI nr
Match: EFH63128.1 (ATP synthase CF0 B subunit [Arabidopsis lyrata subsp. lyrata])

HSP 1 Score: 66.2 bits (160), Expect = 1.3e-07
Identity = 47/73 (64.38%), Postives = 55/73 (75.34%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E AI +QLE AR+R RKVEM+ADQF+  G YSEIEREK NLIN S  K L+QLEN K 
Sbjct: 75  LREGAI-QQLENARARLRKVEMEADQFRVNG-YSEIEREKLNLIN-SIYKTLKQLENYKN 134

Query: 61  ELFVLNNTERLIK 74
           E  +L+N E+LIK
Sbjct: 135 ETILLSNKEQLIK 144

BLAST of MS023004 vs. NCBI nr
Match: PIA34348.1 (hypothetical protein AQUCO_03800145v1 [Aquilegia coerulea])

HSP 1 Score: 65.5 bits (158), Expect = 2.3e-07
Identity = 45/73 (61.64%), Postives = 55/73 (75.34%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E AI+K LEKA++R RK +M+AD+F+    YSEIEREK NLINSS  +NL++LEN K 
Sbjct: 50  LREGAIEK-LEKAKARLRKAKMEADEFR-VNEYSEIEREKRNLINSS-YQNLERLENSKN 109

Query: 61  ELFVLNNTERLIK 74
           E   LNN ERLIK
Sbjct: 110 ETIQLNNKERLIK 119

BLAST of MS023004 vs. NCBI nr
Match: YP_009440143.1 (ATP synthase CF0 B subunit [Gynostemma longipes] >ANI25171.1 ATP synthase CF0 B subunit [Gynostemma pentaphyllum] >ART64986.1 ATP synthase CF0 B subunit [Gynostemma pentaphyllum] >ATG86914.1 ATP synthase CF0 B subunit [Gynostemma longipes])

HSP 1 Score: 65.1 bits (157), Expect = 2.9e-07
Identity = 45/61 (73.77%), Postives = 50/61 (81.97%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E AI+ QLEKARSR RKVEM+ADQF+  G YSEIEREK NLIN STSK+L+QLEN K 
Sbjct: 70  LREGAIE-QLEKARSRLRKVEMEADQFRVNG-YSEIEREKLNLIN-STSKSLEQLENYKN 127

Query: 61  E 62
           E
Sbjct: 130 E 127

BLAST of MS023004 vs. NCBI nr
Match: YP_009439877.1 (ATP synthase CF0 B subunit [Gynostemma caulopterum] >YP_009468847.1 ATP synthase CF0 B subunit [Gynostemma compressum] >ATG86740.1 ATP synthase CF0 B subunit [Gynostemma caulopterum] >AVA29831.1 ATP synthase CF0 B subunit [Gynostemma compressum])

HSP 1 Score: 65.1 bits (157), Expect = 2.9e-07
Identity = 44/61 (72.13%), Postives = 50/61 (81.97%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E A++ QLEKARSR RKVEM+ADQF+  G YSEIEREK NLIN STSK+L+QLEN K 
Sbjct: 70  LREGAVE-QLEKARSRLRKVEMEADQFRVNG-YSEIEREKLNLIN-STSKSLEQLENYKK 127

Query: 61  E 62
           E
Sbjct: 130 E 127

BLAST of MS023004 vs. ExPASy Swiss-Prot
Match: Q4VZP7 (ATP synthase subunit b, chloroplastic OS=Cucumis sativus OX=3659 GN=atpF PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.9e-09
Identity = 40/54 (74.07%), Postives = 46/54 (85.19%), Query Frame = 0

Query: 8   KQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKME 62
           +QLEKAR+R RKVEM+ADQF+  G YSEIEREK NLIN STSK+L+QLEN K E
Sbjct: 76  EQLEKARARLRKVEMEADQFRVNG-YSEIEREKLNLIN-STSKSLEQLENYKNE 127

BLAST of MS023004 vs. ExPASy Swiss-Prot
Match: Q09X31 (ATP synthase subunit b, chloroplastic OS=Morus indica OX=248361 GN=atpF PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 4.3e-09
Identity = 43/61 (70.49%), Postives = 48/61 (78.69%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E A++ QLEKARSR RKVE +ADQF+  G YSEIEREK NLIN STSK L+QLEN K 
Sbjct: 70  LREGALE-QLEKARSRLRKVEKEADQFRVNG-YSEIEREKLNLIN-STSKTLEQLENYKN 127

Query: 61  E 62
           E
Sbjct: 130 E 127

BLAST of MS023004 vs. ExPASy Swiss-Prot
Match: Q68S20 (ATP synthase subunit b, chloroplastic OS=Panax ginseng OX=4054 GN=atpF PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 7.3e-09
Identity = 43/61 (70.49%), Postives = 47/61 (77.05%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E AI+ QLEKAR+R RKVEM+ADQF+  G YSEIEREK N IN STSK L QLEN K 
Sbjct: 70  LREGAIE-QLEKARARLRKVEMEADQFRVNG-YSEIEREKWNFIN-STSKTLDQLENYKN 127

Query: 61  E 62
           E
Sbjct: 130 E 127

BLAST of MS023004 vs. ExPASy Swiss-Prot
Match: B1A921 (ATP synthase subunit b, chloroplastic OS=Carica papaya OX=3649 GN=atpF PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 9.5e-09
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L + AI+ QLEKAR+R RKVEM+A+QF+  G YSEIEREK NLIN STSK L+QLEN K 
Sbjct: 70  LRDGAIE-QLEKARARLRKVEMEAEQFRVNG-YSEIEREKWNLIN-STSKTLEQLENYKN 127

Query: 61  E 62
           E
Sbjct: 130 E 127

BLAST of MS023004 vs. ExPASy Swiss-Prot
Match: B1NWD6 (ATP synthase subunit b, chloroplastic OS=Manihot esculenta OX=3983 GN=atpF PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.2e-08
Identity = 42/61 (68.85%), Postives = 48/61 (78.69%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E AI+ QLEKAR+R RKVE++ADQF+  G YSEIEREK NLIN ST K L+QLEN K 
Sbjct: 70  LREGAIE-QLEKARARLRKVEIEADQFRTNG-YSEIEREKLNLIN-STYKTLEQLENYKN 127

Query: 61  E 62
           E
Sbjct: 130 E 127

BLAST of MS023004 vs. ExPASy TrEMBL
Match: D7KRC8 (ATP synthase CF0 B subunit OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_475583 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 6.4e-08
Identity = 47/73 (64.38%), Postives = 55/73 (75.34%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E AI +QLE AR+R RKVEM+ADQF+  G YSEIEREK NLIN S  K L+QLEN K 
Sbjct: 75  LREGAI-QQLENARARLRKVEMEADQFRVNG-YSEIEREKLNLIN-SIYKTLKQLENYKN 134

Query: 61  ELFVLNNTERLIK 74
           E  +L+N E+LIK
Sbjct: 135 ETILLSNKEQLIK 144

BLAST of MS023004 vs. ExPASy TrEMBL
Match: A0A2G5CSU5 (Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_03800145v1 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.1e-07
Identity = 45/73 (61.64%), Postives = 55/73 (75.34%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E AI+K LEKA++R RK +M+AD+F+    YSEIEREK NLINSS  +NL++LEN K 
Sbjct: 50  LREGAIEK-LEKAKARLRKAKMEADEFR-VNEYSEIEREKRNLINSS-YQNLERLENSKN 109

Query: 61  ELFVLNNTERLIK 74
           E   LNN ERLIK
Sbjct: 110 ETIQLNNKERLIK 119

BLAST of MS023004 vs. ExPASy TrEMBL
Match: A0A191T450 (ATP synthase subunit b, chloroplastic OS=Gynostemma pentaphyllum OX=182084 GN=atpF PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.4e-07
Identity = 45/61 (73.77%), Postives = 50/61 (81.97%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E AI+ QLEKARSR RKVEM+ADQF+  G YSEIEREK NLIN STSK+L+QLEN K 
Sbjct: 70  LREGAIE-QLEKARSRLRKVEMEADQFRVNG-YSEIEREKLNLIN-STSKSLEQLENYKN 127

Query: 61  E 62
           E
Sbjct: 130 E 127

BLAST of MS023004 vs. ExPASy TrEMBL
Match: A0A291IB21 (ATP synthase subunit b, chloroplastic OS=Gynostemma longipes OX=555431 GN=atpF PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.4e-07
Identity = 45/61 (73.77%), Postives = 50/61 (81.97%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E AI+ QLEKARSR RKVEM+ADQF+  G YSEIEREK NLIN STSK+L+QLEN K 
Sbjct: 70  LREGAIE-QLEKARSRLRKVEMEADQFRVNG-YSEIEREKLNLIN-STSKSLEQLENYKN 127

Query: 61  E 62
           E
Sbjct: 130 E 127

BLAST of MS023004 vs. ExPASy TrEMBL
Match: A0A2L0WR24 (ATP synthase subunit b, chloroplastic OS=Gynostemma compressum OX=1348125 GN=atpF PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.4e-07
Identity = 44/61 (72.13%), Postives = 50/61 (81.97%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E A++ QLEKARSR RKVEM+ADQF+  G YSEIEREK NLIN STSK+L+QLEN K 
Sbjct: 70  LREGAVE-QLEKARSRLRKVEMEADQFRVNG-YSEIEREKLNLIN-STSKSLEQLENYKK 127

Query: 61  E 62
           E
Sbjct: 130 E 127

BLAST of MS023004 vs. TAIR 10
Match: ATCG00130.1 (ATPase, F0 complex, subunit B/B', bacterial/chloroplast )

HSP 1 Score: 52.8 bits (125), Expect = 1.4e-07
Identity = 39/61 (63.93%), Postives = 45/61 (73.77%), Query Frame = 0

Query: 1   LHEEAIDKQLEKARSRSRKVEMDADQFQEKGRYSEIEREKTNLINSSTSKNLQQLENKKM 60
           L E AI +QLE AR+R R VE +AD+F+  G YSEIEREK NLIN ST K L+QLEN K 
Sbjct: 70  LREGAI-QQLENARARLRNVETEADKFRVNG-YSEIEREKLNLIN-STYKTLKQLENYKN 127

Query: 61  E 62
           E
Sbjct: 130 E 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CAA9893319.11.2e-0861.97unnamed protein product [Spirodela intermedia][more]
EFH63128.11.3e-0764.38ATP synthase CF0 B subunit [Arabidopsis lyrata subsp. lyrata][more]
PIA34348.12.3e-0761.64hypothetical protein AQUCO_03800145v1 [Aquilegia coerulea][more]
YP_009440143.12.9e-0773.77ATP synthase CF0 B subunit [Gynostemma longipes] >ANI25171.1 ATP synthase CF0 B ... [more]
YP_009439877.12.9e-0772.13ATP synthase CF0 B subunit [Gynostemma caulopterum] >YP_009468847.1 ATP synthase... [more]
Match NameE-valueIdentityDescription
Q4VZP71.9e-0974.07ATP synthase subunit b, chloroplastic OS=Cucumis sativus OX=3659 GN=atpF PE=3 SV... [more]
Q09X314.3e-0970.49ATP synthase subunit b, chloroplastic OS=Morus indica OX=248361 GN=atpF PE=3 SV=... [more]
Q68S207.3e-0970.49ATP synthase subunit b, chloroplastic OS=Panax ginseng OX=4054 GN=atpF PE=3 SV=1[more]
B1A9219.5e-0968.85ATP synthase subunit b, chloroplastic OS=Carica papaya OX=3649 GN=atpF PE=3 SV=1[more]
B1NWD61.2e-0868.85ATP synthase subunit b, chloroplastic OS=Manihot esculenta OX=3983 GN=atpF PE=3 ... [more]
Match NameE-valueIdentityDescription
D7KRC86.4e-0864.38ATP synthase CF0 B subunit OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALY... [more]
A0A2G5CSU51.1e-0761.64Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_03800145v1 PE=3... [more]
A0A191T4501.4e-0773.77ATP synthase subunit b, chloroplastic OS=Gynostemma pentaphyllum OX=182084 GN=at... [more]
A0A291IB211.4e-0773.77ATP synthase subunit b, chloroplastic OS=Gynostemma longipes OX=555431 GN=atpF P... [more]
A0A2L0WR241.4e-0772.13ATP synthase subunit b, chloroplastic OS=Gynostemma compressum OX=1348125 GN=atp... [more]
Match NameE-valueIdentityDescription
ATCG00130.11.4e-0763.93ATPase, F0 complex, subunit B/B', bacterial/chloroplast [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 10..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS023004.1MS023004.1mRNA