MS022712 (gene) Bitter gourd (TR) v1

BLAST of MS022712 vs. NCBI nr
Match: XP_022156968.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Momordica charantia])

HSP 1 Score: 1263.4 bits (3268), Expect = 0.0e+00
Identity = 611/619 (98.71%), Postives = 614/619 (99.19%), Query Frame = 0

Query: 1   MHSAKPLPNLPSPLQFPSPVTLLQATRILFPASPIIPRAATSISGRRHNQTSLPPAEGDD 60
           MHSAKPLPN PSPLQFPSPVTLLQATRILFPASPIIPRAATSISGRRHNQTSLPPAEGDD
Sbjct: 1   MHSAKPLPNSPSPLQFPSPVTLLQATRILFPASPIIPRAATSISGRRHNQTSLPPAEGDD 60

Query: 61  DSVAAFWDYQFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHP 120
           DSVAAFWDYQFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHP
Sbjct: 61  DSVAAFWDYQFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHP 120

Query: 121 LDGHGYLRAFGFENKEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNT 180
           LDGHGYLRAFGFENKEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNT
Sbjct: 121 LDGHGYLRAFGFENKEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNT 180

Query: 181 KVMKNVANTSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG 240
           KVMKNVANTSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG
Sbjct: 181 KVMKNVANTSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG 240

Query: 241 SPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLPTSN 300
           SPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSC AEDMLLPTSN
Sbjct: 241 SPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCKAEDMLLPTSN 300

Query: 301 FTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPM 360
           FTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDT YV+FANRIKLDVIGAMGAVSGISPM
Sbjct: 301 FTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTHYVIFANRIKLDVIGAMGAVSGISPM 360

Query: 361 ISALSVNGSKATCPVYLLPRFAEDGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQ 420
           ISALSVNGSKATCPVYLLPRFAEDGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQ
Sbjct: 361 ISALSVNGSKATCPVYLLPRFAEDGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQ 420

Query: 421 IHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKC 480
           IHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKC
Sbjct: 421 IHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKC 480

Query: 481 SVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVH 540
           SVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKL+TVTNSV 
Sbjct: 481 SVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLNTVTNSVR 540

Query: 541 SWSAGNRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLE 600
           SW AGNRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVA+LE
Sbjct: 541 SWYAGNRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVAKLE 600

Query: 601 VPKHLNFPLGFHGFWAANS 620
           VPKHLNFPLGFHGFWAANS
Sbjct: 601 VPKHLNFPLGFHGFWAANS 619

BLAST of MS022712 vs. NCBI nr
Match: XP_038876141.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Benincasa hispida])

HSP 1 Score: 985.3 bits (2546), Expect = 2.3e-283
Identity = 486/614 (79.15%), Postives = 535/614 (87.13%), Query Frame = 0

Query: 13  PLQFPSPVTLLQATRILFPASPIIPRAATSISGRRHNQTSLPPAEGDDDSVAAFWDYQFL 72
           PLQFPSP+ L   T I FP+S +IP+A   IS    N T +  +  DDDS+AAFWDYQFL
Sbjct: 11  PLQFPSPIKLPPVTVIRFPSS-LIPKA---ISIPTPNTTRI--SINDDDSIAAFWDYQFL 70

Query: 73  FVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGHGYLRAFGF 132
           FVSQR+ET +P VLR VDGA+P DFP GTYYLAGPG+F+DDHGSTVHPLDGHGYLRAF F
Sbjct: 71  FVSQRTETTDPTVLRLVDGAIPPDFPSGTYYLAGPGMFSDDHGSTVHPLDGHGYLRAFVF 130

Query: 133 E--NKEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKVMKNVANTS 192
           E   KEV F AKYV+TEA+MEEH+  TDTWRFTHRGPFSVLKGGK+LGNTKVMKNVANTS
Sbjct: 131 ERKKKEVVFMAKYVKTEAKMEEHDLVTDTWRFTHRGPFSVLKGGKKLGNTKVMKNVANTS 190

Query: 193 VLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGGSPRH----GA 252
           VLRW GRLLCLWEGGDPYEI+P +LDT+GKFSAV         GGC    SP H    GA
Sbjct: 191 VLRWGGRLLCLWEGGDPYEIRPENLDTVGKFSAVH--------GGCD-CDSPSHGGPYGA 250

Query: 253 DLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLPTSNFTFYEF 312
             WRF A LLKP+L+GVFKMPPKRLLSHYKLDAQRNRLLI+SCNAEDMLLPTS+FTFYEF
Sbjct: 251 GFWRFAAELLKPVLYGVFKMPPKRLLSHYKLDAQRNRLLIMSCNAEDMLLPTSHFTFYEF 310

Query: 313 DSNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPMISALSV 372
           DSNFKLLQK+D VIDDHLMIHDWAFTDT Y+LFANRIKLDVIGAMGAVSGISPMISALSV
Sbjct: 311 DSNFKLLQKKDLVIDDHLMIHDWAFTDTHYILFANRIKLDVIGAMGAVSGISPMISALSV 370

Query: 373 NGSKATCPVYLLPRFAEDGNRDWREA-VEVPSRLWLLHVGNAFEVRDDDGNLDIQIHASA 432
           N SK T P+YL+PRF ED NR+ R+A VEVPSRLWLLHVGNAFEV  +DGNLD QIHASA
Sbjct: 371 NTSKPTSPIYLIPRFGEDSNRNLRKAVVEVPSRLWLLHVGNAFEVIHEDGNLDFQIHASA 430

Query: 433 CSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKCSVEPL 492
           CSYQWFNFK+LFGYNWQ+GKLDPSIMNL+ +K++LLPRLVK+SISL+KN KCEKCSVEPL
Sbjct: 431 CSYQWFNFKKLFGYNWQTGKLDPSIMNLNESKSELLPRLVKISISLNKNEKCEKCSVEPL 490

Query: 493 NQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSAG 552
           NQWTK+SDFP INP FSGLK+NYLYAATSSG RRSLPSFPFD +VKLD V N+V +WSAG
Sbjct: 491 NQWTKSSDFPVINPKFSGLKHNYLYAATSSGYRRSLPSFPFDMIVKLDMVMNTVRTWSAG 550

Query: 553 NRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKHL 612
           NRRFVGEPVFVPKG+EEDDGY+LVVEYAVSIQRCYLIILEA++FGEAD +VARLEVPKHL
Sbjct: 551 NRRFVGEPVFVPKGNEEDDGYLLVVEYAVSIQRCYLIILEAQRFGEADGIVARLEVPKHL 609

Query: 613 NFPLGFHGFWAANS 620
           NFPLGFHGFWAAN+
Sbjct: 611 NFPLGFHGFWAANA 609

BLAST of MS022712 vs. NCBI nr
Match: XP_022929872.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita moschata] >KAG6575282.1 Carotenoid cleavage dioxygenase 7, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 979.9 bits (2532), Expect = 9.9e-282
Identity = 476/612 (77.78%), Postives = 529/612 (86.44%), Query Frame = 0

Query: 15  QFPSPVTLLQATRILFPASPIIPRAAT--SISGRRHNQTSLPPAEGDDDSVAAFWDYQFL 74
           QFPSP+ L  AT I FP S +IP+A +  S++  + +  S P     DDS+AAFWDYQFL
Sbjct: 13  QFPSPIKLPPATSIRFPPS-LIPKAISIPSLNTGQISINSPPSIPTVDDSIAAFWDYQFL 72

Query: 75  FVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGHGYLRAFGF 134
           FVSQR+ETA+P VL  V+GA+P +FP GTYYLAGPGLF+DDHGSTVHPLDGHGYLRAF F
Sbjct: 73  FVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRAFIF 132

Query: 135 EN-KEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKVMKNVANTSV 194
           EN KEV F AKYVRTEA+MEEH+ ATDTWRFTHRGPFSVLKGG++LGNTKVMKNVANTSV
Sbjct: 133 ENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVANTSV 192

Query: 195 LRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG---SPRHGADL 254
           L+W G LLCLWEGGDPYEIQP +LDT+GKFS VD        GGC R       RHGA  
Sbjct: 193 LQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVD--------GGCDRDSPSQGGRHGAGF 252

Query: 255 WRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLPTSNFTFYEFDS 314
           WRF A LLKP+L+GVFKMPP+RLLSHYK+DAQRNRLL+ SCNAEDMLLPTS+FTFYEFDS
Sbjct: 253 WRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYEFDS 312

Query: 315 NFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPMISALSVNG 374
           NFKLL+K++FVIDDHLMIHDWAFTDT Y+LFANRIKLD+ GAMGAVSG SPMISAL VN 
Sbjct: 313 NFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGAMGAVSGASPMISALGVNT 372

Query: 375 SKATCPVYLLPRFAE-DGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQIHASACS 434
           SK+T PVYLLPRF E    RDWR+ +++PS LWLLHVGNAFE+ +  GNLD QIHASACS
Sbjct: 373 SKSTSPVYLLPRFGEHHPTRDWRQPIQIPSSLWLLHVGNAFELTNPQGNLDFQIHASACS 432

Query: 435 YQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKCSVEPLNQ 494
           YQWFNF +LFGYNWQ+ KLDPSIMNL+GTK++LLPRLVKVSISLSKNG+CEKCSVEPLNQ
Sbjct: 433 YQWFNFDKLFGYNWQTEKLDPSIMNLNGTKSELLPRLVKVSISLSKNGECEKCSVEPLNQ 492

Query: 495 WTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSAGNR 554
           WTKASDFPAINPTFSG+KNNYLYAATSSG R SLPSFPFDT+VKLDTVT +  +WSAGNR
Sbjct: 493 WTKASDFPAINPTFSGMKNNYLYAATSSGYRESLPSFPFDTIVKLDTVTYTARTWSAGNR 552

Query: 555 RFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKHLNF 614
           RFVGEPVFVPKGD EDDGYVLVVEYAVSIQRCYL+ILEAKK GE DA+VARLEVPKHLNF
Sbjct: 553 RFVGEPVFVPKGDGEDDGYVLVVEYAVSIQRCYLMILEAKKIGEGDAVVARLEVPKHLNF 612

Query: 615 PLGFHGFWAANS 620
           PLGFHGFWAAN+
Sbjct: 613 PLGFHGFWAANT 615

BLAST of MS022712 vs. NCBI nr
Match: XP_023548386.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 976.9 bits (2524), Expect = 8.3e-281
Identity = 476/612 (77.78%), Postives = 527/612 (86.11%), Query Frame = 0

Query: 15  QFPSPVTLLQATRILFPASPIIPRAATSISGRRHNQTSL---PPAEGDDDSVAAFWDYQF 74
           QFPSP+ L  AT I FP S +IP+ A SI      Q S+   P     +DS+AAFWDYQF
Sbjct: 13  QFPSPIKLPPATGIRFPPS-LIPK-AISIPSPNTGQISINSPPSIPTVEDSIAAFWDYQF 72

Query: 75  LFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGHGYLRAFG 134
           LFVSQR+ETA+P VLR V+GA+P +FP GTYYLAGPGLF+DDHGSTVHPLDGHGYLRAF 
Sbjct: 73  LFVSQRTETADPTVLRLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRAFI 132

Query: 135 FEN-KEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKVMKNVANTS 194
           FEN KEV F AKYVRTEA+MEEH+ ATDTWRFTHRGPFSVLKGG++LGNTKVMKNVANTS
Sbjct: 133 FENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVANTS 192

Query: 195 VLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGGSPR---HGAD 254
           VL+W GRLLCLWEGGDPYEIQP +LDT+GKFS VD        GGC R    +   HGA 
Sbjct: 193 VLQWGGRLLCLWEGGDPYEIQPETLDTVGKFSGVD--------GGCDRDSPSQGGLHGAG 252

Query: 255 LWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLPTSNFTFYEFD 314
            WRF A LLKP+L+GVFKMPP+RLLSHYK+DAQRNRLL+ SCNAEDMLLPTS+FTFYEFD
Sbjct: 253 FWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYEFD 312

Query: 315 SNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPMISALSVN 374
           SNFKLL+K++FVIDDHLMIHDWAFTDT Y+LFANRIKLD+ GAMGAVSG SPMISAL VN
Sbjct: 313 SNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGAMGAVSGASPMISALGVN 372

Query: 375 GSKATCPVYLLPRFAE-DGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQIHASAC 434
            SK+T PVYLLPRF E    RDWR+ +++PS LWLLHVGNAFE+ +  GNLD QIHASAC
Sbjct: 373 TSKSTSPVYLLPRFGEHHPTRDWRQPIQIPSSLWLLHVGNAFELTNPQGNLDFQIHASAC 432

Query: 435 SYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKCSVEPLN 494
           SYQWFNF +LFGYNWQ+ KLDPSIMNL+GTK++LLP LVKVSISLSKNG+CEKCSVEPLN
Sbjct: 433 SYQWFNFDKLFGYNWQTEKLDPSIMNLNGTKSELLPHLVKVSISLSKNGECEKCSVEPLN 492

Query: 495 QWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSAGN 554
           QWTKASDFPAINPTFSG+KNNYLYAATSSG R SLPSFPFDT+VKLDTV N+  +WSAGN
Sbjct: 493 QWTKASDFPAINPTFSGMKNNYLYAATSSGYRESLPSFPFDTIVKLDTVMNTARTWSAGN 552

Query: 555 RRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKHLN 614
           RRFVGEPVFVPKGD E+DGYVLVVEYAVSIQRCYL+ILEAKK GE DA+VARLEVPKHLN
Sbjct: 553 RRFVGEPVFVPKGDGEEDGYVLVVEYAVSIQRCYLMILEAKKIGEGDAVVARLEVPKHLN 612

Query: 615 FPLGFHGFWAAN 619
           FPLGFHGFWAAN
Sbjct: 613 FPLGFHGFWAAN 614

BLAST of MS022712 vs. NCBI nr
Match: XP_022973887.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita maxima])

HSP 1 Score: 973.8 bits (2516), Expect = 7.1e-280
Identity = 473/614 (77.04%), Postives = 525/614 (85.50%), Query Frame = 0

Query: 15  QFPSPVTLLQATRILFPASPIIPRAATSISGRR-----HNQTSLPPAEGDDDSVAAFWDY 74
           QFPSP+ L  A  I FP S +IP+A +  S        ++  S+P     DDS+AAFWDY
Sbjct: 13  QFPSPIKLPPAAGIRFPLS-LIPKAISIPSPNTSQISINSPLSIPTV---DDSIAAFWDY 72

Query: 75  QFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGHGYLRA 134
           QFLFVSQR+ETA+P VLR V+GA+P +FP GTYYLAGPGLF+DDHGSTVHPLDGHGYLRA
Sbjct: 73  QFLFVSQRTETADPTVLRLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRA 132

Query: 135 FGFEN-KEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKVMKNVAN 194
           F FEN KEV F A+YVRTEA+MEEH+ ATDTWRFTHRGPFSVLKGG++LGNTKVMKNVAN
Sbjct: 133 FIFENKKEVSFMARYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVAN 192

Query: 195 TSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG---SPRHG 254
           TSVL+W GRLLCLWEGGDPYEIQP +LDT+GKFS VD        GGC R       RHG
Sbjct: 193 TSVLQWGGRLLCLWEGGDPYEIQPETLDTVGKFSGVD--------GGCDRDSPSQGGRHG 252

Query: 255 ADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLPTSNFTFYE 314
           A  WRF A LLKP+L+GVFKMPP+RLLSHYK+DAQRNRLL+ SCNAEDMLLPTS+FTFYE
Sbjct: 253 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 312

Query: 315 FDSNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPMISALS 374
           FDSNF+LL+K++FVIDDHLMIHDWAFTDT Y+LFANRIKLD+ GAMGAVSG SPMIS+L 
Sbjct: 313 FDSNFRLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGAMGAVSGASPMISSLG 372

Query: 375 VNGSKATCPVYLLPRFAE-DGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQIHAS 434
           VN SK+T PVYLLPRF E    RDWR+ +++PS LWLLHVGNAFE+ D  GNLD QIHAS
Sbjct: 373 VNTSKSTSPVYLLPRFGEHHPTRDWRQPIQIPSSLWLLHVGNAFELTDPQGNLDFQIHAS 432

Query: 435 ACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKCSVEP 494
           ACSYQWFNF +LFGYNWQ+GKLDPSIMNL+GTK++LLPRLVKVS  LSKNG+CEKCSVEP
Sbjct: 433 ACSYQWFNFDKLFGYNWQTGKLDPSIMNLNGTKSELLPRLVKVSFRLSKNGECEKCSVEP 492

Query: 495 LNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSA 554
           LNQWTKASDFP INPTFSGLKNNYLYAATSSG R SLPSFPFD +VKLD VTN+  +WSA
Sbjct: 493 LNQWTKASDFPTINPTFSGLKNNYLYAATSSGYRESLPSFPFDMIVKLDMVTNTARTWSA 552

Query: 555 GNRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKH 614
           GNRRFVGEPVFVPKGD EDDGYVLVVEYAVSIQRCYL+ILEAKK GE DA+VARLEVPKH
Sbjct: 553 GNRRFVGEPVFVPKGDGEDDGYVLVVEYAVSIQRCYLMILEAKKIGEGDAVVARLEVPKH 612

Query: 615 LNFPLGFHGFWAAN 619
           LNFPLGFHGFWAAN
Sbjct: 613 LNFPLGFHGFWAAN 614

BLAST of MS022712 vs. ExPASy Swiss-Prot
Match: Q7XJM2 (Carotenoid cleavage dioxygenase 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD7 PE=1 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 1.1e-227
Identity = 385/629 (61.21%), Postives = 474/629 (75.36%), Query Frame = 0

Query: 9   NLPSPLQFPSPVTLLQATRILFPASPIIPRAATSIS----GRRHNQTSLPPAEGDDDSVA 68
           +LP P +F  P+          P     PRAA SIS    G     T L     D+ + +
Sbjct: 2   SLPIPPKFLPPLKSPPIHHHQTPPPLAPPRAAISISIPDTGLGRTGTIL-----DESTSS 61

Query: 69  AFWDYQFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGH 128
           AF DYQ LFVSQRSET EP V++ ++G++P +FP GTYYLAGPGLF DDHGSTVHPLDGH
Sbjct: 62  AFRDYQSLFVSQRSETIEPVVIKPIEGSIPVNFPSGTYYLAGPGLFTDDHGSTVHPLDGH 121

Query: 129 GYLRAFGFENKEVK--FTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKV 188
           GYLRAF  +  + K  FTAKYV+TEA+ EEH+  TDTWRFTHRGPFSVLKGGKR GNTKV
Sbjct: 122 GYLRAFHIDGNKRKATFTAKYVKTEAKKEEHDPVTDTWRFTHRGPFSVLKGGKRFGNTKV 181

Query: 189 MKNVANTSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVD-----VDDDDDGGGGCG 248
           MKNVANTSVL+WAGRLLCLWEGG+PYEI+ GSLDT+G+F+  +      DDDD       
Sbjct: 182 MKNVANTSVLKWAGRLLCLWEGGEPYEIESGSLDTVGRFNVENNGCESCDDDDSSDRDLS 241

Query: 249 RGGSPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLP 308
                  G D+W   A LLKP+L GVFKMPPKR LSHYK+D +R RLL ++CNAEDMLLP
Sbjct: 242 -------GHDIWDTAADLLKPILQGVFKMPPKRFLSHYKVDGRRKRLLTVTCNAEDMLLP 301

Query: 309 TSNFTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGI 368
            SNFTF E+DS FKL+Q ++F IDDH+MIHDWAFTDT Y+LFANR+KL+ IG++ A+ G+
Sbjct: 302 RSNFTFCEYDSEFKLIQTKEFKIDDHMMIHDWAFTDTHYILFANRVKLNPIGSIAAMCGM 361

Query: 369 SPMISALSVNGSKATCPVYLLPRFAED---GNRDWREAVEVPSRLWLLHVGNAFEVRDDD 428
           SPM+SALS+N S  + P+Y+LPRF++    G RDWR  VEV S+LWL+H GNA+E R+D+
Sbjct: 362 SPMVSALSLNPSNESSPIYILPRFSDKYSRGGRDWRVPVEVSSQLWLIHSGNAYETREDN 421

Query: 429 GNLDIQIHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKN 488
           G+L IQI ASACSY+WF+F+++FGY+WQS KLDPS+MNL+    +LLP LVKVS++L   
Sbjct: 422 GDLKIQIQASACSYRWFDFQKMFGYDWQSNKLDPSVMNLNRGDDKLLPHLVKVSMTLDST 481

Query: 489 GKCEKCSVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDT 548
           G C  C VEPLN W K SDFP IN ++SG KN Y+Y+A SSG R  LP FPFD VVK D 
Sbjct: 482 GNCNSCDVEPLNGWNKPSDFPVINSSWSGKKNKYMYSAASSGTRSELPHFPFDMVVKFDL 541

Query: 549 VTNSVHSWSAGNRRFVGEPVFVPK-----GDEEDDGYVLVVEYAVSIQRCYLIILEAKKF 608
            +N V +WS G RRFVGEP+FVPK     G+EEDDGY++VVEYAVS++RCYL+IL+AKK 
Sbjct: 542 DSNLVRTWSTGARRFVGEPMFVPKNSVEEGEEEDDGYIVVVEYAVSVERCYLVILDAKKI 601

Query: 609 GEADAMVARLEVPKHLNFPLGFHGFWAAN 619
           GE+DA+V+RLEVP++L FP+GFHG WA++
Sbjct: 602 GESDAVVSRLEVPRNLTFPMGFHGLWASD 618

BLAST of MS022712 vs. ExPASy Swiss-Prot
Match: Q7XU29 (Carotenoid cleavage dioxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD7 PE=1 SV=2)

HSP 1 Score: 679.5 bits (1752), Expect = 3.6e-194
Identity = 327/578 (56.57%), Postives = 420/578 (72.66%), Query Frame = 0

Query: 47  RHNQTSLPPAEGDDDSVAAFWDYQFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAG 106
           R +  +   A   D   AAFWDY  LF SQR E  +   LR  +GA+P DFP GTYYLAG
Sbjct: 34  RASAAAAAAAAETDTLSAAFWDYNLLFRSQRDECLDSIPLRVTEGAIPPDFPAGTYYLAG 93

Query: 107 PGLFADDHGSTVHPLDGHGYLRAFGFE--NKEVKFTAKYVRTEAQMEEHEAATDTWRFTH 166
           PG+F+DDHGSTVHPLDGHGYLR+F F   ++ + ++A++V T A+ EE      +WRFTH
Sbjct: 94  PGIFSDDHGSTVHPLDGHGYLRSFRFRPGDRTIHYSARFVETAAKREESRDGA-SWRFTH 153

Query: 167 RGPFSVLKGGKRLGNTKVMKNVANTSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAV 226
           RGPFSVL+GGK++GN KVMKNVANTSVLRW GRLLCLWEGG PYE+ P +L+T+G F  +
Sbjct: 154 RGPFSVLQGGKKVGNVKVMKNVANTSVLRWGGRLLCLWEGGQPYEVDPRTLETVGPFDLL 213

Query: 227 DVDDDDD----GGGGCGRGGSPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQ 286
            +   DD          R      G D     ARLL+P+L GVF MP KRLL+HYK+D +
Sbjct: 214 GLAAADDNKATNASAARRPWLQEAGLDA---AARLLRPVLSGVFDMPGKRLLAHYKIDPR 273

Query: 287 RNRLLILSCNAEDMLLPTSNFTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFA 346
           R RLL+++CNAEDMLLP S+FTFYEFD++F L+QK++FV+ DHLMIHDWAFTDT Y+L  
Sbjct: 274 RGRLLMVACNAEDMLLPRSHFTFYEFDAHFDLVQKREFVVPDHLMIHDWAFTDTHYILLG 333

Query: 347 NRIKLDVIGAMGAVSGISPMISALSVNGSKATCPVYLLPRF--AEDGNRDWREAVEVPSR 406
           NRIKLD+ G++ A++G  PMI+AL+V+  + + PVYLLPR    E G RDW   +E PS+
Sbjct: 334 NRIKLDIPGSLLALTGTHPMIAALAVDPRRQSTPVYLLPRSPETEAGGRDWSVPIEAPSQ 393

Query: 407 LWLLHVGNAFEVRDDDGNLDIQIHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKT 466
           +W +HVGNAFE  +  G LD+++H S+CSYQWF+F R+FGYNW   KLDPS MN    K 
Sbjct: 394 MWSVHVGNAFEEANRRGGLDVRLHMSSCSYQWFHFHRMFGYNWHHKKLDPSFMNAAKGK- 453

Query: 467 QLLPRLVKVSISLSKNGKCEKCSVEPL-NQWTKASDFPAINPTFSGLKNNYLYAATSSGN 526
           + LPRLV+V+I L + G+C +CSV  L +Q  + +DFPAINP+++  +N ++YA  +SG+
Sbjct: 454 EWLPRLVQVAIELDRTGECRRCSVRRLSDQHARPADFPAINPSYANQRNRFVYAGAASGS 513

Query: 527 RRSLPSFPFDTVVKLDTVTNSVHSWSAGNRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQ 586
           RR LP FPFD+VVK+D    S   WS   R+FVGEPVFVP G  ED GYVL+VEYAVS  
Sbjct: 514 RRFLPYFPFDSVVKVDVSDGSARWWSTDGRKFVGEPVFVPTGGGEDGGYVLLVEYAVSKH 573

Query: 587 RCYLIILEAKKFGEADAMVARLEVPKHLNFPLGFHGFW 616
           RC+L++L+AKK G  +A+VA+LEVPK+L FP+GFHGFW
Sbjct: 574 RCHLVVLDAKKIGTENALVAKLEVPKNLTFPMGFHGFW 606

BLAST of MS022712 vs. ExPASy Swiss-Prot
Match: P74334 (Apocarotenoid-15,15'-oxygenase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1541 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 1.0e-31
Identity = 140/538 (26.02%), Postives = 227/538 (42.19%), Query Frame = 0

Query: 89  VDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGHGYLRAFGFE-NKEVKFTAKYVRTE 148
           V+G++P D   GT Y  GPGL         HP DG G + AF F  +  V F +K+VRT+
Sbjct: 38  VEGSIPPDLQ-GTLYRNGPGLLEIGDRPLKHPFDGDGMVTAFKFPGDGRVHFQSKFVRTQ 97

Query: 149 AQMEEHEAATDTWRFTHRGPFSVLKGG---KRLGNTKVMKNVANTSVLRWAGRLLCLWEG 208
             +EE +A     +  +RG F     G   K + + + +KN+ANT++  W  RLL LWEG
Sbjct: 98  GYVEEQKAG----KMIYRGVFGSQPAGGWLKTIFDLR-LKNIANTNITYWGDRLLALWEG 157

Query: 209 GDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGGSPRHGADLWRFVARLLKPLLHGVF 268
           G P+ ++P +L TIG          DD GG    G                         
Sbjct: 158 GQPHRLEPSNLATIGL---------DDLGGILAEG------------------------- 217

Query: 269 KMPPKRLLSHYKLDA----QRNRLLILSCNAEDMLLPTSNFTFYEFDSNFKLLQKQDFVI 328
               + L +H ++D        +   ++ + +  L  +S  T  E D   KLL+++    
Sbjct: 218 ----QPLSAHPRIDPASTFDGGQPCYVTFSIKSSL--SSTLTLLELDPQGKLLRQKTETF 277

Query: 329 DDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPMISALSVNGSKATCPVYLLPR 388
                IHD+A T    +   N + L+ +  +  + G    +             + L+PR
Sbjct: 278 PGFAFIHDFAITPHYAIFLQNNVTLNGLPYLFGLRGAGECVQF----HPDKPAQIILVPR 337

Query: 389 FAEDGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQIHASACSYQWFNFKRLFGYN 448
              DG    R  V+     ++ H  NAFE   ++G +   I  S C      +  L   +
Sbjct: 338 ---DGGEIKRIPVQAG---FVFHHANAFE---ENGKI---ILDSIC------YNSLPQVD 397

Query: 449 WQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPAINPT 508
                   +  NLD        +L + +I  +        +VE     ++  +FP ++P 
Sbjct: 398 TDGDFRSTNFDNLDP------GQLWRFTIDPA------AATVEKQLMVSRCCEFPVVHPQ 457

Query: 509 FSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSAGNRRFVGEPVFVPK-- 568
             G    Y+Y   +     S  + P   ++K+D  + +    S     F GEP+FVP+  
Sbjct: 458 QVGRPYRYVYMGAA---HHSTGNAPLQAILKVDLESGTETLRSFAPHGFAGEPIFVPRPG 488

Query: 569 GDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKHLNFPLGFHGFWA 617
           G  EDDG++L + Y   + R  L+IL+A+        +A L++  H+ +PL  HG WA
Sbjct: 518 GVAEDDGWLLCLIYKADLHRSELVILDAQDI--TAPAIATLKLKHHIPYPL--HGSWA 488

BLAST of MS022712 vs. ExPASy Swiss-Prot
Match: Q9LRM7 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED6 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.1e-16
Identity = 120/524 (22.90%), Postives = 213/524 (40.65%), Query Frame = 0

Query: 119 HPLDGHGYLRA--FGFENKEVKFTAKYVRTEAQMEE-----------------HEAATDT 178
           H  DG G + A   GF+N +V ++ +Y +T   ++E                 H      
Sbjct: 147 HLFDGDGMIHAVSIGFDN-QVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARL 206

Query: 179 WRFTHRGPFSVLKGGKRLGNTKVMKNVANTSVLRWAGRLLCLWEGGDPYEIQ---PGSLD 238
             FT R    ++ G + +G       VAN  V+ + GRLL + E   PY+++    G L+
Sbjct: 207 ALFTARAGIGLVDGTRGMG-------VANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLE 266

Query: 239 TIGKFSAVDVDDDDDGGGGCGRGGSPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYK 298
           TIG+F     DD  D                                       +++H K
Sbjct: 267 TIGRFG---FDDQIDSS-------------------------------------VIAHPK 326

Query: 299 LDAQRNRLLILSCNAEDMLLPTSNFTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTRY 358
           +DA    L  LS N    +L   +  + +F++  K  +  +  + +  MIHD+A T+  +
Sbjct: 327 VDATTGDLHTLSYN----VLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITE-NF 386

Query: 359 VLFANRIKLDVIGAMGAVSGISPMISALSVNGSKATCPVYLLPRFAEDGNRDWREAVEVP 418
           V+  ++  +  +  M  + G SP+I    V    A   V L  +     + +W   V+VP
Sbjct: 387 VVIPDQQMVFKLSEM--IRGGSPVI---YVKEKMARFGV-LSKQDLTGSDINW---VDVP 446

Query: 419 SRLWLLHVGNAFEVRDDDGNLDIQIHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGT 478
              +  H+ NA+E R ++G+  I +  S  S                     +I +  G 
Sbjct: 447 D-CFCFHLWNAWEERTEEGDPVIVVIGSCMS------------------PPDTIFSESGE 506

Query: 479 KTQLLPRLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSG 538
            T++   L ++ +++       K  V  +N      +   IN ++ G K+ ++Y A +  
Sbjct: 507 PTRV--ELSEIRLNMRTKESNRKVIVTGVN-----LEAGHINRSYVGRKSQFVYIAIAD- 566

Query: 539 NRRSLPSFPFDTVVKLDTVTNSVHSWSAGNRRFVGEPVFVPKGD-EEDDGYVLVVEYAVS 598
                P      + K+D    +V  ++ G  RF GEP FVP+G+ EED GYV+       
Sbjct: 567 -----PWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPEGEGEEDKGYVMGFVRDEE 570

Query: 599 IQRCYLIILEAKKFGEADAMVARLEVPKHLNFPLGFHGFWAANS 620
                 ++++A    +    VA + +P+ +  P GFHG + + +
Sbjct: 627 KDESEFVVVDATDMKQ----VAAVRLPERV--PYGFHGTFVSEN 570

BLAST of MS022712 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.8e-10
Identity = 105/510 (20.59%), Postives = 189/510 (37.06%), Query Frame = 0

Query: 119 HPLDGHGYLRAFGFENKEVKFTAKYVRTEAQMEEHEAATDTW------RFTHRGPFSVL- 178
           H  DG G + A  FE+    +  ++ +T   ++E +     +         H G   ++ 
Sbjct: 169 HFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLML 228

Query: 179 ---KGGKRLGNTKVMKNVANTSVLRWAGRLLCLWEGGDPYEIQ---PGSLDTIGKFSAVD 238
              +    + +      VAN  ++ + GRLL + E   PY++Q    G L T+G+F    
Sbjct: 229 FYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRF---- 288

Query: 239 VDDDDDGGGGCGRGGSPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLL 298
              D DG                                      +++H K+D +   L 
Sbjct: 289 ---DFDG---------------------------------QLESTMIAHPKVDPESGELF 348

Query: 299 ILSCNAEDMLLPTSNFTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKL 358
            LS +    ++      ++ F  +       +  +D   M+HD+A T+   V+   ++  
Sbjct: 349 ALSYD----VVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQV-- 408

Query: 359 DVIGAMGAVSGISPMISALSVNGSKATCPVYLLPRFAED-GNRDWREAVEVPSRLWLLHV 418
            V      + G SP+     V          +L ++AED  N  W +A +     +  H+
Sbjct: 409 -VFKLPEMIRGGSPV-----VYDKNKVARFGILDKYAEDSSNIKWIDAPD----CFCFHL 468

Query: 419 GNAFEVRDDDGNLDIQIHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRL 478
            NA+E  + D  + I     +C                   + P     + +   L   L
Sbjct: 469 WNAWEEPETDEVVVI----GSC-------------------MTPPDSIFNESDENLKSVL 528

Query: 479 VKVSISLSKNGKCEKCSVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSF 538
            ++ ++L K G+  +  +          +   +N    G K  + Y A +       P  
Sbjct: 529 SEIRLNL-KTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAE------PWP 586

Query: 539 PFDTVVKLDTVTNSVHSWSAGNRRFVGEPVFVP-KGDEEDDGYVLVVEYAVSIQRCYLII 598
                 K+D  T  V     G+ R+ GEP+F+P +G EED+GY+L   +     +  L I
Sbjct: 589 KVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGEEDEGYILCFVHDEKTWKSELQI 586

Query: 599 LEAKKFGEADAMVARLEVPKHLNFPLGFHG 614
           + A    E +A V   ++P  +  P GFHG
Sbjct: 649 VNAVSL-EVEATV---KLPSRV--PYGFHG 586

BLAST of MS022712 vs. ExPASy TrEMBL
Match: A0A6J1DS65 (carotenoid cleavage dioxygenase 7, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023796 PE=3 SV=1)

HSP 1 Score: 1263.4 bits (3268), Expect = 0.0e+00
Identity = 611/619 (98.71%), Postives = 614/619 (99.19%), Query Frame = 0

Query: 1   MHSAKPLPNLPSPLQFPSPVTLLQATRILFPASPIIPRAATSISGRRHNQTSLPPAEGDD 60
           MHSAKPLPN PSPLQFPSPVTLLQATRILFPASPIIPRAATSISGRRHNQTSLPPAEGDD
Sbjct: 1   MHSAKPLPNSPSPLQFPSPVTLLQATRILFPASPIIPRAATSISGRRHNQTSLPPAEGDD 60

Query: 61  DSVAAFWDYQFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHP 120
           DSVAAFWDYQFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHP
Sbjct: 61  DSVAAFWDYQFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHP 120

Query: 121 LDGHGYLRAFGFENKEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNT 180
           LDGHGYLRAFGFENKEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNT
Sbjct: 121 LDGHGYLRAFGFENKEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNT 180

Query: 181 KVMKNVANTSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG 240
           KVMKNVANTSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG
Sbjct: 181 KVMKNVANTSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG 240

Query: 241 SPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLPTSN 300
           SPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSC AEDMLLPTSN
Sbjct: 241 SPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCKAEDMLLPTSN 300

Query: 301 FTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPM 360
           FTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDT YV+FANRIKLDVIGAMGAVSGISPM
Sbjct: 301 FTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTHYVIFANRIKLDVIGAMGAVSGISPM 360

Query: 361 ISALSVNGSKATCPVYLLPRFAEDGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQ 420
           ISALSVNGSKATCPVYLLPRFAEDGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQ
Sbjct: 361 ISALSVNGSKATCPVYLLPRFAEDGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQ 420

Query: 421 IHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKC 480
           IHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKC
Sbjct: 421 IHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKC 480

Query: 481 SVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVH 540
           SVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKL+TVTNSV 
Sbjct: 481 SVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLNTVTNSVR 540

Query: 541 SWSAGNRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLE 600
           SW AGNRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVA+LE
Sbjct: 541 SWYAGNRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVAKLE 600

Query: 601 VPKHLNFPLGFHGFWAANS 620
           VPKHLNFPLGFHGFWAANS
Sbjct: 601 VPKHLNFPLGFHGFWAANS 619

BLAST of MS022712 vs. ExPASy TrEMBL
Match: A0A6J1ENV6 (carotenoid cleavage dioxygenase 7, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111436349 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 4.8e-282
Identity = 476/612 (77.78%), Postives = 529/612 (86.44%), Query Frame = 0

Query: 15  QFPSPVTLLQATRILFPASPIIPRAAT--SISGRRHNQTSLPPAEGDDDSVAAFWDYQFL 74
           QFPSP+ L  AT I FP S +IP+A +  S++  + +  S P     DDS+AAFWDYQFL
Sbjct: 13  QFPSPIKLPPATSIRFPPS-LIPKAISIPSLNTGQISINSPPSIPTVDDSIAAFWDYQFL 72

Query: 75  FVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGHGYLRAFGF 134
           FVSQR+ETA+P VL  V+GA+P +FP GTYYLAGPGLF+DDHGSTVHPLDGHGYLRAF F
Sbjct: 73  FVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRAFIF 132

Query: 135 EN-KEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKVMKNVANTSV 194
           EN KEV F AKYVRTEA+MEEH+ ATDTWRFTHRGPFSVLKGG++LGNTKVMKNVANTSV
Sbjct: 133 ENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVANTSV 192

Query: 195 LRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG---SPRHGADL 254
           L+W G LLCLWEGGDPYEIQP +LDT+GKFS VD        GGC R       RHGA  
Sbjct: 193 LQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVD--------GGCDRDSPSQGGRHGAGF 252

Query: 255 WRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLPTSNFTFYEFDS 314
           WRF A LLKP+L+GVFKMPP+RLLSHYK+DAQRNRLL+ SCNAEDMLLPTS+FTFYEFDS
Sbjct: 253 WRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYEFDS 312

Query: 315 NFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPMISALSVNG 374
           NFKLL+K++FVIDDHLMIHDWAFTDT Y+LFANRIKLD+ GAMGAVSG SPMISAL VN 
Sbjct: 313 NFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGAMGAVSGASPMISALGVNT 372

Query: 375 SKATCPVYLLPRFAE-DGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQIHASACS 434
           SK+T PVYLLPRF E    RDWR+ +++PS LWLLHVGNAFE+ +  GNLD QIHASACS
Sbjct: 373 SKSTSPVYLLPRFGEHHPTRDWRQPIQIPSSLWLLHVGNAFELTNPQGNLDFQIHASACS 432

Query: 435 YQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKCSVEPLNQ 494
           YQWFNF +LFGYNWQ+ KLDPSIMNL+GTK++LLPRLVKVSISLSKNG+CEKCSVEPLNQ
Sbjct: 433 YQWFNFDKLFGYNWQTEKLDPSIMNLNGTKSELLPRLVKVSISLSKNGECEKCSVEPLNQ 492

Query: 495 WTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSAGNR 554
           WTKASDFPAINPTFSG+KNNYLYAATSSG R SLPSFPFDT+VKLDTVT +  +WSAGNR
Sbjct: 493 WTKASDFPAINPTFSGMKNNYLYAATSSGYRESLPSFPFDTIVKLDTVTYTARTWSAGNR 552

Query: 555 RFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKHLNF 614
           RFVGEPVFVPKGD EDDGYVLVVEYAVSIQRCYL+ILEAKK GE DA+VARLEVPKHLNF
Sbjct: 553 RFVGEPVFVPKGDGEDDGYVLVVEYAVSIQRCYLMILEAKKIGEGDAVVARLEVPKHLNF 612

Query: 615 PLGFHGFWAANS 620
           PLGFHGFWAAN+
Sbjct: 613 PLGFHGFWAANT 615

BLAST of MS022712 vs. ExPASy TrEMBL
Match: A0A6J1I8R3 (carotenoid cleavage dioxygenase 7, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472478 PE=3 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 3.4e-280
Identity = 473/614 (77.04%), Postives = 525/614 (85.50%), Query Frame = 0

Query: 15  QFPSPVTLLQATRILFPASPIIPRAATSISGRR-----HNQTSLPPAEGDDDSVAAFWDY 74
           QFPSP+ L  A  I FP S +IP+A +  S        ++  S+P     DDS+AAFWDY
Sbjct: 13  QFPSPIKLPPAAGIRFPLS-LIPKAISIPSPNTSQISINSPLSIPTV---DDSIAAFWDY 72

Query: 75  QFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGHGYLRA 134
           QFLFVSQR+ETA+P VLR V+GA+P +FP GTYYLAGPGLF+DDHGSTVHPLDGHGYLRA
Sbjct: 73  QFLFVSQRTETADPTVLRLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRA 132

Query: 135 FGFEN-KEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKVMKNVAN 194
           F FEN KEV F A+YVRTEA+MEEH+ ATDTWRFTHRGPFSVLKGG++LGNTKVMKNVAN
Sbjct: 133 FIFENKKEVSFMARYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVAN 192

Query: 195 TSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG---SPRHG 254
           TSVL+W GRLLCLWEGGDPYEIQP +LDT+GKFS VD        GGC R       RHG
Sbjct: 193 TSVLQWGGRLLCLWEGGDPYEIQPETLDTVGKFSGVD--------GGCDRDSPSQGGRHG 252

Query: 255 ADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLPTSNFTFYE 314
           A  WRF A LLKP+L+GVFKMPP+RLLSHYK+DAQRNRLL+ SCNAEDMLLPTS+FTFYE
Sbjct: 253 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 312

Query: 315 FDSNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPMISALS 374
           FDSNF+LL+K++FVIDDHLMIHDWAFTDT Y+LFANRIKLD+ GAMGAVSG SPMIS+L 
Sbjct: 313 FDSNFRLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGAMGAVSGASPMISSLG 372

Query: 375 VNGSKATCPVYLLPRFAE-DGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQIHAS 434
           VN SK+T PVYLLPRF E    RDWR+ +++PS LWLLHVGNAFE+ D  GNLD QIHAS
Sbjct: 373 VNTSKSTSPVYLLPRFGEHHPTRDWRQPIQIPSSLWLLHVGNAFELTDPQGNLDFQIHAS 432

Query: 435 ACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKCSVEP 494
           ACSYQWFNF +LFGYNWQ+GKLDPSIMNL+GTK++LLPRLVKVS  LSKNG+CEKCSVEP
Sbjct: 433 ACSYQWFNFDKLFGYNWQTGKLDPSIMNLNGTKSELLPRLVKVSFRLSKNGECEKCSVEP 492

Query: 495 LNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSA 554
           LNQWTKASDFP INPTFSGLKNNYLYAATSSG R SLPSFPFD +VKLD VTN+  +WSA
Sbjct: 493 LNQWTKASDFPTINPTFSGLKNNYLYAATSSGYRESLPSFPFDMIVKLDMVTNTARTWSA 552

Query: 555 GNRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKH 614
           GNRRFVGEPVFVPKGD EDDGYVLVVEYAVSIQRCYL+ILEAKK GE DA+VARLEVPKH
Sbjct: 553 GNRRFVGEPVFVPKGDGEDDGYVLVVEYAVSIQRCYLMILEAKKIGEGDAVVARLEVPKH 612

Query: 615 LNFPLGFHGFWAAN 619
           LNFPLGFHGFWAAN
Sbjct: 613 LNFPLGFHGFWAAN 614

BLAST of MS022712 vs. ExPASy TrEMBL
Match: T1UMH0 (Carotenoid cleavage dioxygenase OS=Cucumis melo OX=3656 GN=CCD7 PE=2 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 1.5e-275
Identity = 463/609 (76.03%), Postives = 523/609 (85.88%), Query Frame = 0

Query: 13  PLQFPSPVTLLQATRILFPASPIIPRAATSISGRRHNQTSLPPAEGDDDSVAAFWDYQFL 72
           PLQFPSPV L   T I FP+S + P+ A SIS    N+ S+   + DDDS+AAFWDYQFL
Sbjct: 11  PLQFPSPVKLPPPTGIRFPSS-LTPK-AISISTPNTNRISI--NKNDDDSIAAFWDYQFL 70

Query: 73  FVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGHGYLRAFGF 132
           F+SQR+ET  PAVLR VDGA+P DFP GTYYL GPG+F+DDHGSTVHPLDGHGYLRAF F
Sbjct: 71  FISQRTETENPAVLRLVDGAIPPDFPSGTYYLTGPGMFSDDHGSTVHPLDGHGYLRAFAF 130

Query: 133 ENKEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKVMKNVANTSVL 192
           E  EV F AKYV+TEA+MEEH+  TD WRFTHRGPFSVLKGGK+LGNTKVMKNVANTSVL
Sbjct: 131 EKDEVAFMAKYVKTEAKMEEHDPETDRWRFTHRGPFSVLKGGKKLGNTKVMKNVANTSVL 190

Query: 193 RWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGGSPRHGADLWRFV 252
           RW GRLLCLWEGGDPYEI+P  LDT+GKFSA D   D D      RGG+  +    WRF 
Sbjct: 191 RWGGRLLCLWEGGDPYEIRPEDLDTVGKFSAFDGGGDHDSP---SRGGT--YDGGFWRFA 250

Query: 253 ARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLPTSNFTFYEFDSNFKL 312
           A LLKP+L+GVFKMPPKRLLSHYK+DAQRNRLLI+SCNAEDMLLPTS+FTFYEFDSNFKL
Sbjct: 251 AELLKPVLYGVFKMPPKRLLSHYKVDAQRNRLLIMSCNAEDMLLPTSHFTFYEFDSNFKL 310

Query: 313 LQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPMISALSVNGSKAT 372
           LQK+D VIDDHLMIHDWAFTD  Y+LFANRIKLDVIGAMGA++G+SPMISALSVN +K+T
Sbjct: 311 LQKKDLVIDDHLMIHDWAFTDNHYILFANRIKLDVIGAMGAITGVSPMISALSVNTNKST 370

Query: 373 CPVYLLPRFAEDGNRDWREA-VEVPSRLWLLHVGNAFE-VRDDDGNLDIQIHASACSYQW 432
            P+YL+PRF ED N+D +E  VEVPSRLW+LHVGNAFE + ++DG LD QIHAS+CSYQW
Sbjct: 371 SPIYLIPRFGEDSNKDCKETIVEVPSRLWVLHVGNAFEIIHEEDGTLDFQIHASSCSYQW 430

Query: 433 FNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKCSVEPLNQWTK 492
           FNFK+LFGYNWQ+GKLDPS+MN + TK++  P LVKV ISLSKNGKCEKCSVEPL+QW K
Sbjct: 431 FNFKKLFGYNWQTGKLDPSVMNPNETKSKQFPHLVKVCISLSKNGKCEKCSVEPLSQWPK 490

Query: 493 ASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSAGNRRFV 552
           +SDFP INP FSGLK+NYLYAATSSGNRRSLPSFPFD +VKLD V ++V +W AGNRRFV
Sbjct: 491 SSDFPIINPKFSGLKHNYLYAATSSGNRRSLPSFPFDMIVKLDAVPSTVRTWFAGNRRFV 550

Query: 553 GEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKHLNFPLG 612
           GEPVFVPKGD EDDGY+LVVEYAVSIQRCYLIIL+A++FGEAD +VAR EVPKHLNFPLG
Sbjct: 551 GEPVFVPKGDGEDDGYLLVVEYAVSIQRCYLIILDAQRFGEADGVVARFEVPKHLNFPLG 610

Query: 613 FHGFWAANS 620
           FHGFWAAN+
Sbjct: 611 FHGFWAANT 610

BLAST of MS022712 vs. ExPASy TrEMBL
Match: A0A0A0KAN6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106700 PE=3 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 7.4e-275
Identity = 464/611 (75.94%), Postives = 524/611 (85.76%), Query Frame = 0

Query: 13  PLQFPSPVTL-LQATRILFPASPIIPRAATSISGRRHNQTSLPPAEGDDDSVAAFWDYQF 72
           PLQFPSP+ L   +T I FP+S + P+ A SIS    ++ S+   + DDDS+AAFWDYQF
Sbjct: 11  PLQFPSPIKLPPPSTGIRFPSS-LTPK-AISISTPNTHRISINKNDDDDDSIAAFWDYQF 70

Query: 73  LFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGHGYLRAFG 132
           LF+SQR+ET  PAVLR VDGA+P+DFP GTYYL GPG+F+DDHGSTVHPLDGHGYLRAF 
Sbjct: 71  LFISQRTETENPAVLRLVDGAIPSDFPSGTYYLTGPGMFSDDHGSTVHPLDGHGYLRAFV 130

Query: 133 FE-NKEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKVMKNVANTS 192
           FE + EV F AKYV+TEA+MEEH+  TD WRFTHRGPFSVLKGGK+LGNTKVMKNVANTS
Sbjct: 131 FEKDHEVTFMAKYVKTEAKMEEHDPETDRWRFTHRGPFSVLKGGKKLGNTKVMKNVANTS 190

Query: 193 VLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGGSPRHGADLWR 252
           VLRW GRLLCLWEGGDPYEI+   LDT+GKF A    DD D      RGG   +    WR
Sbjct: 191 VLRWGGRLLCLWEGGDPYEIRAEDLDTVGKFCAFHGGDDHDSP---SRGGG--YDGGFWR 250

Query: 253 FVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLPTSNFTFYEFDSNF 312
           F A LLKP+L+GVFKMPPKRLLSHYK+DAQRNRLL++SCNAEDMLLPTS+FTFYEFDSNF
Sbjct: 251 FAAELLKPVLYGVFKMPPKRLLSHYKVDAQRNRLLVMSCNAEDMLLPTSHFTFYEFDSNF 310

Query: 313 KLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGISPMISALSVNGSK 372
           KLLQK+D VIDDHLMIHDWAFTD  Y+LFANRIKLDVIGAM A+SGISPMISALSVN +K
Sbjct: 311 KLLQKKDMVIDDHLMIHDWAFTDNHYILFANRIKLDVIGAMSAISGISPMISALSVNTNK 370

Query: 373 ATCPVYLLPRFAEDGNR-DWREA-VEVPSRLWLLHVGNAFEVRDDDGNLDIQIHASACSY 432
           +T P+YL+PRF ED  + DW+E  VEVPSRLWLLHVGNAFE   +DG LD +IHAS+CSY
Sbjct: 371 STSPIYLIPRFGEDSKKDDWKETIVEVPSRLWLLHVGNAFETIHEDGTLDFEIHASSCSY 430

Query: 433 QWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKNGKCEKCSVEPLNQW 492
           QWFNFK+LFGYNWQ+GKLDPS+MN + TK++  P LVKVSISLSKNGKCEKCSVEPLNQW
Sbjct: 431 QWFNFKKLFGYNWQTGKLDPSVMNPNETKSKQFPHLVKVSISLSKNGKCEKCSVEPLNQW 490

Query: 493 TKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSAGNRR 552
            K+SDFP INP FSGLK+NYLYAATSSGNRRSLPSFPFD +VKLDTVTN+V +W AGNRR
Sbjct: 491 PKSSDFPVINPKFSGLKHNYLYAATSSGNRRSLPSFPFDMIVKLDTVTNTVRTWFAGNRR 550

Query: 553 FVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKHLNFP 612
           FVGEPVFVPKGD+EDDGY+LVVEYAVSIQRCYLIILEA++FGEAD +VAR EVPKHLNFP
Sbjct: 551 FVGEPVFVPKGDKEDDGYLLVVEYAVSIQRCYLIILEAQRFGEADGVVARFEVPKHLNFP 610

Query: 613 LGFHGFWAANS 620
           +GFHGFWAAN+
Sbjct: 611 IGFHGFWAANT 614

BLAST of MS022712 vs. TAIR 10
Match: AT2G44990.1 (carotenoid cleavage dioxygenase 7 )

HSP 1 Score: 757.3 bits (1954), Expect = 9.7e-219
Identity = 375/629 (59.62%), Postives = 466/629 (74.09%), Query Frame = 0

Query: 9   NLPSPLQFPSPVTLLQATRILFPASPIIPRAATSIS----GRRHNQTSLPPAEGDDDSVA 68
           +LP P +F  P+          P     PRAA SIS    G     T L     D+ + +
Sbjct: 2   SLPIPPKFLPPLKSPPIHHHQTPPPLAPPRAAISISIPDTGLGRTGTIL-----DESTSS 61

Query: 69  AFWDYQFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGH 128
           AF DYQ LFVSQRSET EP V++ ++G++P +FP GTYYLAGPGLF DDHGSTVHPLDGH
Sbjct: 62  AFRDYQSLFVSQRSETIEPVVIKPIEGSIPVNFPSGTYYLAGPGLFTDDHGSTVHPLDGH 121

Query: 129 GYLRAFGFENKEVK--FTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKV 188
           GYLRAF  +  + K  FTAKYV+TEA+ EEH+  TDTWRFTHRGPFSVLKGGKR GNTKV
Sbjct: 122 GYLRAFHIDGNKRKATFTAKYVKTEAKKEEHDPVTDTWRFTHRGPFSVLKGGKRFGNTKV 181

Query: 189 MKNVANTSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVD-----VDDDDDGGGGCG 248
           MKNVANTSVL+WAGRLLCLWEGG+PYEI+ GSLDT+G+F+  +      DDDD       
Sbjct: 182 MKNVANTSVLKWAGRLLCLWEGGEPYEIESGSLDTVGRFNVENNGCESCDDDDSSDRDLS 241

Query: 249 RGGSPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCNAEDMLLP 308
                  G D+W   A LLKP+L GVFKMPPKR LSHYK+D +R RLL ++CNAEDMLLP
Sbjct: 242 -------GHDIWDTAADLLKPILQGVFKMPPKRFLSHYKVDGRRKRLLTVTCNAEDMLLP 301

Query: 309 TSNFTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKLDVIGAMGAVSGI 368
            SNFTF E+DS FKL+Q ++F IDDH+MIHDWAFTDT Y+LFANR+KL+ IG++ A+ G+
Sbjct: 302 RSNFTFCEYDSEFKLIQTKEFKIDDHMMIHDWAFTDTHYILFANRVKLNPIGSIAAMCGM 361

Query: 369 SPMISALSVNGSKATCPVYLLPRFAED---GNRDWREAVEVPSRLWLLHVGNAFEVRDDD 428
           SPM+SALS+N S  + P+Y+LPRF++    G RDWR  VEV S+LWL+H GNA+E R+D+
Sbjct: 362 SPMVSALSLNPSNESSPIYILPRFSDKYSRGGRDWRVPVEVSSQLWLIHSGNAYETREDN 421

Query: 429 GNLDIQIHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSISLSKN 488
           G+L IQI ASACSY+WF+F+++FGY+WQS KLDPS+MNL+    +LLP LVKVS++L   
Sbjct: 422 GDLKIQIQASACSYRWFDFQKMFGYDWQSNKLDPSVMNLNRGDDKLLPHLVKVSMTLDST 481

Query: 489 GKCEKCSVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDT 548
           G C  C VEPLN W K SDFP IN ++SG KN Y+Y+A SSG R  LP FPFD VVK D 
Sbjct: 482 GNCNSCDVEPLNGWNKPSDFPVINSSWSGKKNKYMYSAASSGTRSELPHFPFDMVVKFDL 541

Query: 549 VTNSVHSWSAGNRRFVGEPVFVPK-----GDEEDDGYVLVVEYAVSIQRCYLIILEAKKF 608
            +N V +WS G RRFVGEP+FVPK     G+EEDDGY++VVEYAVS++RCYL+IL+AKK 
Sbjct: 542 DSNLVRTWSTGARRFVGEPMFVPKNSVEEGEEEDDGYIVVVEYAVSVERCYLVILDAKKI 601

Query: 609 GEADAMVA---RLEVPKHLNFPLGFHGFW 616
           GE+DA+V+   ++ + K +N+ +  H F+
Sbjct: 602 GESDAVVSSVNKVYIAK-INYIICVHSFY 617

BLAST of MS022712 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 89.0 bits (219), Expect = 1.5e-17
Identity = 120/524 (22.90%), Postives = 213/524 (40.65%), Query Frame = 0

Query: 119 HPLDGHGYLRA--FGFENKEVKFTAKYVRTEAQMEE-----------------HEAATDT 178
           H  DG G + A   GF+N +V ++ +Y +T   ++E                 H      
Sbjct: 147 HLFDGDGMIHAVSIGFDN-QVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARL 206

Query: 179 WRFTHRGPFSVLKGGKRLGNTKVMKNVANTSVLRWAGRLLCLWEGGDPYEIQ---PGSLD 238
             FT R    ++ G + +G       VAN  V+ + GRLL + E   PY+++    G L+
Sbjct: 207 ALFTARAGIGLVDGTRGMG-------VANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLE 266

Query: 239 TIGKFSAVDVDDDDDGGGGCGRGGSPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYK 298
           TIG+F     DD  D                                       +++H K
Sbjct: 267 TIGRFG---FDDQIDSS-------------------------------------VIAHPK 326

Query: 299 LDAQRNRLLILSCNAEDMLLPTSNFTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTRY 358
           +DA    L  LS N    +L   +  + +F++  K  +  +  + +  MIHD+A T+  +
Sbjct: 327 VDATTGDLHTLSYN----VLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITE-NF 386

Query: 359 VLFANRIKLDVIGAMGAVSGISPMISALSVNGSKATCPVYLLPRFAEDGNRDWREAVEVP 418
           V+  ++  +  +  M  + G SP+I    V    A   V L  +     + +W   V+VP
Sbjct: 387 VVIPDQQMVFKLSEM--IRGGSPVI---YVKEKMARFGV-LSKQDLTGSDINW---VDVP 446

Query: 419 SRLWLLHVGNAFEVRDDDGNLDIQIHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGT 478
              +  H+ NA+E R ++G+  I +  S  S                     +I +  G 
Sbjct: 447 D-CFCFHLWNAWEERTEEGDPVIVVIGSCMS------------------PPDTIFSESGE 506

Query: 479 KTQLLPRLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSG 538
            T++   L ++ +++       K  V  +N      +   IN ++ G K+ ++Y A +  
Sbjct: 507 PTRV--ELSEIRLNMRTKESNRKVIVTGVN-----LEAGHINRSYVGRKSQFVYIAIAD- 566

Query: 539 NRRSLPSFPFDTVVKLDTVTNSVHSWSAGNRRFVGEPVFVPKGD-EEDDGYVLVVEYAVS 598
                P      + K+D    +V  ++ G  RF GEP FVP+G+ EED GYV+       
Sbjct: 567 -----PWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPEGEGEEDKGYVMGFVRDEE 570

Query: 599 IQRCYLIILEAKKFGEADAMVARLEVPKHLNFPLGFHGFWAANS 620
                 ++++A    +    VA + +P+ +  P GFHG + + +
Sbjct: 627 KDESEFVVVDATDMKQ----VAAVRLPERV--PYGFHGTFVSEN 570

BLAST of MS022712 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 68.2 bits (165), Expect = 2.7e-11
Identity = 105/510 (20.59%), Postives = 189/510 (37.06%), Query Frame = 0

Query: 119 HPLDGHGYLRAFGFENKEVKFTAKYVRTEAQMEEHEAATDTW------RFTHRGPFSVL- 178
           H  DG G + A  FE+    +  ++ +T   ++E +     +         H G   ++ 
Sbjct: 169 HFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLML 228

Query: 179 ---KGGKRLGNTKVMKNVANTSVLRWAGRLLCLWEGGDPYEIQ---PGSLDTIGKFSAVD 238
              +    + +      VAN  ++ + GRLL + E   PY++Q    G L T+G+F    
Sbjct: 229 FYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRF---- 288

Query: 239 VDDDDDGGGGCGRGGSPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLL 298
              D DG                                      +++H K+D +   L 
Sbjct: 289 ---DFDG---------------------------------QLESTMIAHPKVDPESGELF 348

Query: 299 ILSCNAEDMLLPTSNFTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTRYVLFANRIKL 358
            LS +    ++      ++ F  +       +  +D   M+HD+A T+   V+   ++  
Sbjct: 349 ALSYD----VVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQV-- 408

Query: 359 DVIGAMGAVSGISPMISALSVNGSKATCPVYLLPRFAED-GNRDWREAVEVPSRLWLLHV 418
            V      + G SP+     V          +L ++AED  N  W +A +     +  H+
Sbjct: 409 -VFKLPEMIRGGSPV-----VYDKNKVARFGILDKYAEDSSNIKWIDAPD----CFCFHL 468

Query: 419 GNAFEVRDDDGNLDIQIHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRL 478
            NA+E  + D  + I     +C                   + P     + +   L   L
Sbjct: 469 WNAWEEPETDEVVVI----GSC-------------------MTPPDSIFNESDENLKSVL 528

Query: 479 VKVSISLSKNGKCEKCSVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSF 538
            ++ ++L K G+  +  +          +   +N    G K  + Y A +       P  
Sbjct: 529 SEIRLNL-KTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAE------PWP 586

Query: 539 PFDTVVKLDTVTNSVHSWSAGNRRFVGEPVFVP-KGDEEDDGYVLVVEYAVSIQRCYLII 598
                 K+D  T  V     G+ R+ GEP+F+P +G EED+GY+L   +     +  L I
Sbjct: 589 KVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGEEDEGYILCFVHDEKTWKSELQI 586

Query: 599 LEAKKFGEADAMVARLEVPKHLNFPLGFHG 614
           + A    E +A V   ++P  +  P GFHG
Sbjct: 649 VNAVSL-EVEATV---KLPSRV--PYGFHG 586

BLAST of MS022712 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 48.5 bits (114), Expect = 2.2e-05
Identity = 33/118 (27.97%), Postives = 52/118 (44.07%), Query Frame = 0

Query: 497 INPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSAGNRRFVGEPVFV 556
           +N    G K  Y Y A +       P        K+D  T  +  +  G  ++ GEP+F+
Sbjct: 538 VNRNLLGRKTRYAYLALTE------PWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFL 597

Query: 557 PKGD-EEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKHLNFPLGFHG 614
           P GD EED GY++V  +     +  L ++ A        + A + +P  +  P GFHG
Sbjct: 598 PSGDGEEDGGYIMVFVHDEEKVKSELQLINAVNM----KLEATVTLPSRV--PYGFHG 643

BLAST of MS022712 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 46.2 bits (108), Expect = 1.1e-04
Identity = 34/120 (28.33%), Postives = 52/120 (43.33%), Query Frame = 0

Query: 497 INPTFSGLKNNYLYAATSSGNRRSLPSFPFDTVVKLDTVTNSVHSWSAGNRRFVGEPVFV 556
           +N    G K  Y Y A +       P        K+D  T  V +   G +++ GEP F+
Sbjct: 470 VNRNLLGRKTRYAYLAIAE------PWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFL 529

Query: 557 PKG---DEEDDGYVLVVEYAVSIQRCYLIILEAKKFGEADAMVARLEVPKHLNFPLGFHG 614
           P+G   D EDDGY++   +        L I+ A        + A +++P  +  P GFHG
Sbjct: 530 PRGLESDGEDDGYIMSFVHDEESWESELHIVNAVTL----ELEATVKLPSRV--PYGFHG 577