MS022509 (gene) Bitter gourd (TR) v1

BLAST of MS022509 vs. NCBI nr
Match: XP_038876374.1 (haloacid dehalogenase-like hydrolase domain-containing protein 3 [Benincasa hispida] >XP_038876375.1 haloacid dehalogenase-like hydrolase domain-containing protein 3 [Benincasa hispida] >XP_038876376.1 haloacid dehalogenase-like hydrolase domain-containing protein 3 [Benincasa hispida])

HSP 1 Score: 505.4 bits (1300), Expect = 2.9e-139
Identity = 235/249 (94.38%), Postives = 243/249 (97.59%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           MSLLSKLRCITIDVTGTL+AYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMA N
Sbjct: 1   MSLLSKLRCITIDVTGTLLAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMAKN 60

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG+AAKMPNI+WWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVF DSQ
Sbjct: 61  YPCFGYAAKMPNIIWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFEDSQ 120

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRWVR+QGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIY+I
Sbjct: 121 PFLRWVREQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYEI 180

Query: 181 ALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDLV 240
           A+ERAGNI PEEALHIGDSLRKDY+PAKSVGMH LLLDRFKT DAEEWRKSGAVVLPDLV
Sbjct: 181 AIERAGNIAPEEALHIGDSLRKDYIPAKSVGMHGLLLDRFKTLDAEEWRKSGAVVLPDLV 240

Query: 241 AAQHWLQNN 250
           AA+ WLQNN
Sbjct: 241 AARDWLQNN 249

BLAST of MS022509 vs. NCBI nr
Match: XP_031743322.1 (haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucumis sativus])

HSP 1 Score: 505.0 bits (1299), Expect = 3.7e-139
Identity = 234/249 (93.98%), Postives = 243/249 (97.59%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           MSLLSKLRCITIDVTGTL+AYKGELGDYYCMAAKSVGLPCPDYKRVHEGFK AYKEMA N
Sbjct: 1   MSLLSKLRCITIDVTGTLLAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKFAYKEMAKN 60

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG+AAKMPNI+WWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVF DSQ
Sbjct: 61  YPCFGYAAKMPNIIWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFEDSQ 120

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRWVR+QGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIY+I
Sbjct: 121 PFLRWVREQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYEI 180

Query: 181 ALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDLV 240
           A+ERAGNI PEEALHIGDSLRKDY+PAKSVGMH LLLDRFKT+DAEEWRKSGAVVLPDLV
Sbjct: 181 AIERAGNIAPEEALHIGDSLRKDYIPAKSVGMHGLLLDRFKTSDAEEWRKSGAVVLPDLV 240

Query: 241 AAQHWLQNN 250
           AA+ WLQNN
Sbjct: 241 AAREWLQNN 249

BLAST of MS022509 vs. NCBI nr
Match: KAA0052427.1 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 505.0 bits (1299), Expect = 3.7e-139
Identity = 234/249 (93.98%), Postives = 244/249 (97.99%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           MSLLSKLRCITIDVTGTL+AYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMA N
Sbjct: 1   MSLLSKLRCITIDVTGTLLAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMAKN 60

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG+AAKMPNI+WWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVF DSQ
Sbjct: 61  YPCFGYAAKMPNIIWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFEDSQ 120

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRWVR+QGLMVGI+SNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIY+I
Sbjct: 121 PFLRWVREQGLMVGIISNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYEI 180

Query: 181 ALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDLV 240
           A+ERAGNI PEEALHIGDSLRKDY+PAKSVGMH LLLDRFKT+DAEEWRKSGAVVLPDLV
Sbjct: 181 AIERAGNIAPEEALHIGDSLRKDYLPAKSVGMHGLLLDRFKTSDAEEWRKSGAVVLPDLV 240

Query: 241 AAQHWLQNN 250
           AA+ WLQNN
Sbjct: 241 AARDWLQNN 249

BLAST of MS022509 vs. NCBI nr
Match: XP_004134528.1 (haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucumis sativus] >KGN49528.1 hypothetical protein Csa_003539 [Cucumis sativus])

HSP 1 Score: 503.8 bits (1296), Expect = 8.3e-139
Identity = 233/249 (93.57%), Postives = 243/249 (97.59%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           MSLLSKLRCITIDVTGTL+AYKGELGDYYCMAAKSVGLPCPDY+RVHEGFK AYKEMA N
Sbjct: 1   MSLLSKLRCITIDVTGTLLAYKGELGDYYCMAAKSVGLPCPDYRRVHEGFKFAYKEMAKN 60

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG+AAKMPNI+WWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVF DSQ
Sbjct: 61  YPCFGYAAKMPNIIWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFEDSQ 120

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRWVR+QGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIY+I
Sbjct: 121 PFLRWVREQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYEI 180

Query: 181 ALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDLV 240
           A+ERAGNI PEEALHIGDSLRKDY+PAKSVGMH LLLDRFKT+DAEEWRKSGAVVLPDLV
Sbjct: 181 AIERAGNIAPEEALHIGDSLRKDYIPAKSVGMHGLLLDRFKTSDAEEWRKSGAVVLPDLV 240

Query: 241 AAQHWLQNN 250
           AA+ WLQNN
Sbjct: 241 AAREWLQNN 249

BLAST of MS022509 vs. NCBI nr
Match: XP_022926076.1 (haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucurbita moschata] >XP_023544714.1 haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucurbita pepo subsp. pepo] >XP_023544715.1 haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucurbita pepo subsp. pepo] >KAG6581523.1 Haloacid dehalogenase-like hydrolase domain-containing protein 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034816.1 Haloacid dehalogenase-like hydrolase domain-containing protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 500.7 bits (1288), Expect = 7.1e-138
Identity = 232/249 (93.17%), Postives = 243/249 (97.59%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           MSLLSKLRCITIDVTGTL+AYKGELGDYYCMAAKSVGLPCPDYKR+HEGFKLAYKEMA N
Sbjct: 1   MSLLSKLRCITIDVTGTLIAYKGELGDYYCMAAKSVGLPCPDYKRMHEGFKLAYKEMAKN 60

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG+AAKMPNIVWWKTCVRDSFIRAGYDYDEETF+KVFRRIYASFGSSAPYKVF DSQ
Sbjct: 61  YPCFGYAAKMPNIVWWKTCVRDSFIRAGYDYDEETFDKVFRRIYASFGSSAPYKVFEDSQ 120

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRWVR QGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIY+I
Sbjct: 121 PFLRWVRQQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYEI 180

Query: 181 ALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDLV 240
           A+ERAGNI PEEALHIGDSLRKDY+PAKSVGMH LLLDRF+T+DAEEWRKSGAVVLPDL+
Sbjct: 181 AIERAGNIAPEEALHIGDSLRKDYIPAKSVGMHGLLLDRFETSDAEEWRKSGAVVLPDLM 240

Query: 241 AAQHWLQNN 250
           AA+ WLQNN
Sbjct: 241 AAKEWLQNN 249

BLAST of MS022509 vs. ExPASy Swiss-Prot
Match: Q9CYW4 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Mus musculus OX=10090 GN=Hdhd3 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.8e-28
Identity = 73/212 (34.43%), Postives = 119/212 (56.13%), Query Frame = 0

Query: 6   KLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATNYPCFG 65
           ++R +T DV  TL+  +  +G+ Y   A++ G+   D   V + F+ AY+  + N+P +G
Sbjct: 6   QMRLLTWDVKDTLIKLRRPVGEEYASKARAHGVVVEDI-TVEQAFRQAYRAQSHNFPNYG 65

Query: 66  HAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQPFLRW 125
            +  + +  WWK  V  +F  AG   D +    V  ++Y  F S   ++V   ++  L+ 
Sbjct: 66  LSRGLTSRQWWKDVVLHTFRLAGVP-DAQAMTPVADQLYEDFSSPFTWQVLEGAEMTLKG 125

Query: 126 VRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKIALERA 185
            R +GL + +VSN + R +D IL  LGL +   +DF + S   G  KPDPRI++ AL+RA
Sbjct: 126 CRKRGLKLAVVSNFDRRLED-ILTGLGLRE--HFDFVLTSEAVGCPKPDPRIFREALQRA 185

Query: 186 GNIEPEEALHIGDSLRKDYVPAKSVGMHALLL 218
             +EP  A H+GDS   DY  +++VGMH+ L+
Sbjct: 186 -CVEPAVAAHVGDSYLCDYQGSQAVGMHSFLV 211

BLAST of MS022509 vs. ExPASy Swiss-Prot
Match: Q5E9D6 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Bos taurus OX=9913 GN=HDHD3 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.4e-27
Identity = 72/212 (33.96%), Postives = 117/212 (55.19%), Query Frame = 0

Query: 6   KLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATNYPCFG 65
           +LR +T DV  TL+  +  +G  Y   A++ GL   +   + + FK AYK  + ++P +G
Sbjct: 6   QLRLLTWDVKDTLLRLRHPVGVEYATKARAHGLEV-EATALGQAFKQAYKAQSQSFPNYG 65

Query: 66  HAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQPFLRW 125
               + +  WW   V+ +F +AG   D +    +  ++Y  F S + ++V   ++  LR 
Sbjct: 66  LGHGLTSHQWWLDLVQQTFHQAGV-RDAQAVAPIAEQLYKDFSSPSTWQVLEGAEATLRG 125

Query: 126 VRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKIALERA 185
            R +GL + +VSN + R +D IL  +GL +   +DF + S   G  KPDPRI+  AL  A
Sbjct: 126 CRKRGLKLAVVSNFDRRLED-ILEGVGLRE--HFDFVLTSEAAGWPKPDPRIFHEALHLA 185

Query: 186 GNIEPEEALHIGDSLRKDYVPAKSVGMHALLL 218
             +EP    HIGDS ++DY  A++VGMH+ L+
Sbjct: 186 -QVEPAVGAHIGDSYQRDYKGARAVGMHSFLV 211

BLAST of MS022509 vs. ExPASy Swiss-Prot
Match: Q5HZL9 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Xenopus laevis OX=8355 GN=hdhd3 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.9e-21
Identity = 65/222 (29.28%), Postives = 111/222 (50.00%), Query Frame = 0

Query: 7   LRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATNYPCFGH 66
           LR IT DV  TL+  +  +G  Y   AK  GL C +   +   F+ AY+  +  +P +G 
Sbjct: 3   LRLITWDVKDTLLRVRVPVGQQYYAEAKKRGL-CVNPGTLETSFRNAYRSHSRLFPNYGL 62

Query: 67  AAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQPFLRWV 126
           A  M +  WW   V  +F  +G + D +T + + +++Y  F ++  + + P ++  L   
Sbjct: 63  AQGMSSRQWWLDVVLQTFRLSGIE-DSDTVQSLAKQLYQDFSTAHNWALVPGAREALDSC 122

Query: 127 RDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKIALERAG 186
            + GL + ++SN + R ++++           +DF V +   GV KP   I+  AL  A 
Sbjct: 123 TNLGLRMAVISNFDRRLEELLRQCC---LERYFDFVVTAESAGVAKPHLGIFHKALSLA- 182

Query: 187 NIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEW 229
            + P +A+H+GD    DY  A+ VGMH+ L+   KT    +W
Sbjct: 183 KVPPHQAVHVGDDYVNDYCAARMVGMHSYLIHP-KTPPKLQW 217

BLAST of MS022509 vs. ExPASy Swiss-Prot
Match: Q94915 (Rhythmically expressed gene 2 protein OS=Drosophila melanogaster OX=7227 GN=Reg-2 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 5.6e-21
Identity = 68/227 (29.96%), Postives = 110/227 (48.46%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           M  LS+ R IT DVT TL+ ++   G  Y       G  C D   + + FK  + +M  +
Sbjct: 1   MRSLSRFRLITFDVTNTLLQFRTTPGKQYGEIGALFGARC-DNNELAKNFKANWYKMNRD 60

Query: 61  YPCFGHAA--KMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPD 120
           YP FG     +M    WW+  +  +F  +G    +E        +   + +S  ++    
Sbjct: 61  YPNFGRDTNPQMEWQQWWRKLIAGTFAESGAAIPDEKLHNFSNHLIELYKTSICWQPCNG 120

Query: 121 SQPFLRWVR----DQGLMVGIVSNAEYRYQDVILPALGLNQGSE--WDFGVFSGLEGVEK 180
           S   L+ +R     +   +G+++N + R     LP L  N   +   DF + S     EK
Sbjct: 121 SVELLQQLRKELKPEKCKLGVIANFDPR-----LPTLLQNTKLDQYLDFAINSYEVQAEK 180

Query: 181 PDPRIYKIALERAG--NIEPEEALHIGDSLRKDYVPAKSVGMHALLL 218
           PDP+I++ A+E++G  N++PEE LHIGD    DY+ AK +G H+ L+
Sbjct: 181 PDPQIFQKAMEKSGLKNLKPEECLHIGDGPTTDYLAAKELGWHSALV 221

BLAST of MS022509 vs. ExPASy Swiss-Prot
Match: Q08CY5 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Xenopus tropicalis OX=8364 GN=hdhd3 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.8e-12
Identity = 41/139 (29.50%), Postives = 71/139 (51.08%), Query Frame = 0

Query: 7   LRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATNYPCFGH 66
           LR IT D+  TL+  +  +G  Y   AK  GL C D   +   F+ AY+  +  +P +G 
Sbjct: 3   LRLITWDIKDTLLRVRVPVGQQYFAEAKRQGL-CMDPGSLETSFRNAYRTHSRLFPNYGL 62

Query: 67  AAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQPFLRWV 126
           A  M +  WW   V  +F  +G + D+ET   V +++Y  F ++  + V P ++  L   
Sbjct: 63  AQGMDSRQWWLDVVLQTFRLSGAE-DDETVRSVAQQLYQDFSTARNWAVVPGAREALDSC 122

Query: 127 RDQGLMVGIVSNAEYRYQD 146
           +  GL + ++SN + R ++
Sbjct: 123 KGLGLKMAVISNFDRRLEE 139

BLAST of MS022509 vs. ExPASy TrEMBL
Match: A0A5A7UD59 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G00220 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 1.8e-139
Identity = 234/249 (93.98%), Postives = 244/249 (97.99%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           MSLLSKLRCITIDVTGTL+AYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMA N
Sbjct: 1   MSLLSKLRCITIDVTGTLLAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMAKN 60

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG+AAKMPNI+WWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVF DSQ
Sbjct: 61  YPCFGYAAKMPNIIWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFEDSQ 120

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRWVR+QGLMVGI+SNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIY+I
Sbjct: 121 PFLRWVREQGLMVGIISNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYEI 180

Query: 181 ALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDLV 240
           A+ERAGNI PEEALHIGDSLRKDY+PAKSVGMH LLLDRFKT+DAEEWRKSGAVVLPDLV
Sbjct: 181 AIERAGNIAPEEALHIGDSLRKDYLPAKSVGMHGLLLDRFKTSDAEEWRKSGAVVLPDLV 240

Query: 241 AAQHWLQNN 250
           AA+ WLQNN
Sbjct: 241 AARDWLQNN 249

BLAST of MS022509 vs. ExPASy TrEMBL
Match: A0A0A0KJ36 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G532590 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.0e-139
Identity = 233/249 (93.57%), Postives = 243/249 (97.59%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           MSLLSKLRCITIDVTGTL+AYKGELGDYYCMAAKSVGLPCPDY+RVHEGFK AYKEMA N
Sbjct: 1   MSLLSKLRCITIDVTGTLLAYKGELGDYYCMAAKSVGLPCPDYRRVHEGFKFAYKEMAKN 60

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG+AAKMPNI+WWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVF DSQ
Sbjct: 61  YPCFGYAAKMPNIIWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFEDSQ 120

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRWVR+QGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIY+I
Sbjct: 121 PFLRWVREQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYEI 180

Query: 181 ALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDLV 240
           A+ERAGNI PEEALHIGDSLRKDY+PAKSVGMH LLLDRFKT+DAEEWRKSGAVVLPDLV
Sbjct: 181 AIERAGNIAPEEALHIGDSLRKDYIPAKSVGMHGLLLDRFKTSDAEEWRKSGAVVLPDLV 240

Query: 241 AAQHWLQNN 250
           AA+ WLQNN
Sbjct: 241 AAREWLQNN 249

BLAST of MS022509 vs. ExPASy TrEMBL
Match: A0A6J1EDV5 (haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111433300 PE=4 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 3.4e-138
Identity = 232/249 (93.17%), Postives = 243/249 (97.59%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           MSLLSKLRCITIDVTGTL+AYKGELGDYYCMAAKSVGLPCPDYKR+HEGFKLAYKEMA N
Sbjct: 1   MSLLSKLRCITIDVTGTLIAYKGELGDYYCMAAKSVGLPCPDYKRMHEGFKLAYKEMAKN 60

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG+AAKMPNIVWWKTCVRDSFIRAGYDYDEETF+KVFRRIYASFGSSAPYKVF DSQ
Sbjct: 61  YPCFGYAAKMPNIVWWKTCVRDSFIRAGYDYDEETFDKVFRRIYASFGSSAPYKVFEDSQ 120

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRWVR QGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIY+I
Sbjct: 121 PFLRWVRQQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYEI 180

Query: 181 ALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDLV 240
           A+ERAGNI PEEALHIGDSLRKDY+PAKSVGMH LLLDRF+T+DAEEWRKSGAVVLPDL+
Sbjct: 181 AIERAGNIAPEEALHIGDSLRKDYIPAKSVGMHGLLLDRFETSDAEEWRKSGAVVLPDLM 240

Query: 241 AAQHWLQNN 250
           AA+ WLQNN
Sbjct: 241 AAKEWLQNN 249

BLAST of MS022509 vs. ExPASy TrEMBL
Match: A0A6J1ISF9 (haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111478007 PE=4 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 7.6e-138
Identity = 231/249 (92.77%), Postives = 243/249 (97.59%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           MSLLSKLRCITIDVTGTL+AYKGELGDYYCMAAKSVGLPCPDYKR+HEGFKLAYKEMA N
Sbjct: 1   MSLLSKLRCITIDVTGTLIAYKGELGDYYCMAAKSVGLPCPDYKRMHEGFKLAYKEMAKN 60

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG+AAKMPNIVWWKTCVRDSFIRAGYDYDEETF+KVFRRIYASFGSSAPYKVF DSQ
Sbjct: 61  YPCFGYAAKMPNIVWWKTCVRDSFIRAGYDYDEETFDKVFRRIYASFGSSAPYKVFEDSQ 120

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRWVR QGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIY+I
Sbjct: 121 PFLRWVRQQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYEI 180

Query: 181 ALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDLV 240
           A+ERAGNI PEEALHIGDSLRKDY+PAKSVGMH LLLDRF+T+DAE+WRKSGAVVLPDL+
Sbjct: 181 AIERAGNIAPEEALHIGDSLRKDYIPAKSVGMHGLLLDRFETSDAEDWRKSGAVVLPDLM 240

Query: 241 AAQHWLQNN 250
           AA+ WLQNN
Sbjct: 241 AAKEWLQNN 249

BLAST of MS022509 vs. ExPASy TrEMBL
Match: W9RRW5 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Morus notabilis OX=981085 GN=L484_003872 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.6e-132
Identity = 219/249 (87.95%), Postives = 237/249 (95.18%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           MS+LS LRCIT+DVTGTL+AYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYK++A  
Sbjct: 1   MSILSTLRCITVDVTGTLLAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKDVAQK 60

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG+AAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPY VFPDSQ
Sbjct: 61  YPCFGYAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYTVFPDSQ 120

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRWVR+QGL VG++SNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKP+PRIY+I
Sbjct: 121 PFLRWVREQGLQVGLISNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPNPRIYEI 180

Query: 181 ALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDLV 240
           AL+RAGN+ P+E LHIGDS+RKDYVPAKSVGMHALLLDRFKT DAEEWRKSGA VLPD V
Sbjct: 181 ALQRAGNVAPQEVLHIGDSMRKDYVPAKSVGMHALLLDRFKTKDAEEWRKSGATVLPDFV 240

Query: 241 AAQHWLQNN 250
           AA+ WL ++
Sbjct: 241 AAKEWLTSD 249

BLAST of MS022509 vs. TAIR 10
Match: AT5G44730.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 429.5 bits (1103), Expect = 1.9e-120
Identity = 193/250 (77.20%), Postives = 229/250 (91.60%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           +SLLSKLRCIT+DVTGTL+AYKGELGDYYCMAAK++GLPCPDYKRVHEGFKLAY +MA  
Sbjct: 3   VSLLSKLRCITVDVTGTLIAYKGELGDYYCMAAKAIGLPCPDYKRVHEGFKLAYTDMAQK 62

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG  AKMPNIVWWKTCVRDSF++AGY+YDEETFEK+FRRIY++FGS+APY VF DSQ
Sbjct: 63  YPCFGFHAKMPNIVWWKTCVRDSFVKAGYEYDEETFEKIFRRIYSTFGSAAPYSVFQDSQ 122

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRW R +GL+VG+VSNAEYRYQ+VILP+ GL++ +EWDFGVFSG+EG+EKPDPRI+ +
Sbjct: 123 PFLRWARRKGLIVGLVSNAEYRYQEVILPSFGLSK-AEWDFGVFSGIEGIEKPDPRIFTL 182

Query: 181 ALERAG-NIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDL 240
           ALERAG NI PEE LHIGDS+RKDYVPAKS+GMHALL+DRFKT  A++W ++GA+VLPDL
Sbjct: 183 ALERAGNNIAPEEVLHIGDSMRKDYVPAKSIGMHALLVDRFKTEAAKDWIEAGAIVLPDL 242

Query: 241 VAAQHWLQNN 250
           VA Q  L+++
Sbjct: 243 VAVQQLLESD 251

BLAST of MS022509 vs. TAIR 10
Match: AT5G44730.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 429.5 bits (1103), Expect = 1.9e-120
Identity = 193/250 (77.20%), Postives = 229/250 (91.60%), Query Frame = 0

Query: 1   MSLLSKLRCITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYKEMATN 60
           +SLLSKLRCIT+DVTGTL+AYKGELGDYYCMAAK++GLPCPDYKRVHEGFKLAY +MA  
Sbjct: 3   VSLLSKLRCITVDVTGTLIAYKGELGDYYCMAAKAIGLPCPDYKRVHEGFKLAYTDMAQK 62

Query: 61  YPCFGHAAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQ 120
           YPCFG  AKMPNIVWWKTCVRDSF++AGY+YDEETFEK+FRRIY++FGS+APY VF DSQ
Sbjct: 63  YPCFGFHAKMPNIVWWKTCVRDSFVKAGYEYDEETFEKIFRRIYSTFGSAAPYSVFQDSQ 122

Query: 121 PFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKI 180
           PFLRW R +GL+VG+VSNAEYRYQ+VILP+ GL++ +EWDFGVFSG+EG+EKPDPRI+ +
Sbjct: 123 PFLRWARRKGLIVGLVSNAEYRYQEVILPSFGLSK-AEWDFGVFSGIEGIEKPDPRIFTL 182

Query: 181 ALERAG-NIEPEEALHIGDSLRKDYVPAKSVGMHALLLDRFKTADAEEWRKSGAVVLPDL 240
           ALERAG NI PEE LHIGDS+RKDYVPAKS+GMHALL+DRFKT  A++W ++GA+VLPDL
Sbjct: 183 ALERAGNNIAPEEVLHIGDSMRKDYVPAKSIGMHALLVDRFKTEAAKDWIEAGAIVLPDL 242

Query: 241 VAAQHWLQNN 250
           VA Q  L+++
Sbjct: 243 VAVQQLLESD 251

BLAST of MS022509 vs. TAIR 10
Match: AT1G14310.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.7e-09
Identity = 48/206 (23.30%), Postives = 88/206 (42.72%), Query Frame = 0

Query: 10  ITIDVTGTLMAYKGELGDYYCMAAKSVGLPCPDYKRVHEGFKLAYK---EMATNYPCFGH 69
           + +D  GTL+     + + Y    +  GL       + EGFK  +         Y   G 
Sbjct: 45  LLLDAGGTLLQLSKPVHETYASLGQKYGLKTTP-AEIKEGFKRVFSAPWPEKLRYQGDGR 104

Query: 70  AAKMPNIVWWKTCVRDSFIRAGYDYDEETFEKVFRRIYASFGSSAPYKVFPDSQPFLRWV 129
                   +WK  V ++   +  DY        F  +Y  + +   + +   +   +  +
Sbjct: 105 P-------FWKLVVSEATGCSDNDY--------FEDVYQYYANGEAWHLPEGAYETMSLL 164

Query: 130 RDQGLMVGIVSNAEYRYQDVILPALGLNQGSEWDFGVFSGLEGVEKPDPRIYKIALERAG 189
           +D G+ + +VSN + R + ++     LN    +D  + S   G EKPD RI+K ALE+  
Sbjct: 165 KDAGVKMAVVSNFDTRLRKLL---KDLNVIDMFDAVIVSAEVGYEKPDERIFKSALEQI- 224

Query: 190 NIEPEEALHIGDSLRKDYVPAKSVGM 213
           +++   A+H+GD    D   A ++G+
Sbjct: 225 SVDVNRAVHVGDDEGADKGGANAIGI 230

BLAST of MS022509 vs. TAIR 10
Match: AT2G41250.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-08
Identity = 37/119 (31.09%), Postives = 59/119 (49.58%), Query Frame = 0

Query: 100 FRRIYASFGSSAPYKVF-PDSQPFLRWVRDQGLMVGIVSNAEYRYQDVILPALGLNQGSE 159
           F  +Y+ F +   +K+  PD+    + +++ G+ V IVSN + R    + P L   +  +
Sbjct: 158 FEELYSYFTTEQAWKLCDPDAGKVFKAIKEAGVKVAIVSNFDTR----LRPLLRALRCED 217

Query: 160 W-DFGVFSGLEGVEKPDPRIYKIALERAGNIEPEEALHIGDSLRKDYVPAKSVGMHALL 217
           W D    S     EKP+P I+  A E    + PE+A+H+GD  R D   A+  G  A L
Sbjct: 218 WFDAVAVSAEVEAEKPNPTIFLKACELL-EVNPEDAVHVGDDRRNDVWGARDAGCDAWL 271