MS022377 (gene) Bitter gourd (TR) v1

Overview


Name	MS022377
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	mitogen-activated protein kinase kinase kinase 18-like
Location	scaffold47: 2875452 .. 2875892 (+)
RNA-Seq Expression	MS022377
Synteny	MS022377

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAACAGGGGATTCTTTCTACTCTGTTCCACCCATCCGTTGTGGGCTACAGGGGATTCGATTTAACGAGAGAGAACGGCAAGGTAATGTATAATCTCTTCATGGAGTACGTCGCCGGCGGTTCCCTCGGCGACGAAATCGTCCGGCGTGGCGGTCGGATTAAGGAGGCGGCGGCGGGGTTCTACACGCGTCAAATTGTCAGGGGATTGGAGTATTTGCACGCGCGAGGATTGGTGCATTGCGATATTAAAGCTAGGAATATTTTGGTCGGTGAAGATGGGGTGAAGATTGCTGATTTCGGCTGCTCCAAATGGGTCTCTCAGCCGGCGGCGATCGGCGGGACACCGATGTTCATGGCGCCGGAGGTGGCGCGTGGGGAAGAACAGGGGTTTTCCTCTGACATTTGGGCGCTTGGGTGTACCTTAATCGAAATGGTCACCGGC

mRNA sequence

GAACAGGGGATTCTTTCTACTCTGTTCCACCCATCCGTTGTGGGCTACAGGGGATTCGATTTAACGAGAGAGAACGGCAAGGTAATGTATAATCTCTTCATGGAGTACGTCGCCGGCGGTTCCCTCGGCGACGAAATCGTCCGGCGTGGCGGTCGGATTAAGGAGGCGGCGGCGGGGTTCTACACGCGTCAAATTGTCAGGGGATTGGAGTATTTGCACGCGCGAGGATTGGTGCATTGCGATATTAAAGCTAGGAATATTTTGGTCGGTGAAGATGGGGTGAAGATTGCTGATTTCGGCTGCTCCAAATGGGTCTCTCAGCCGGCGGCGATCGGCGGGACACCGATGTTCATGGCGCCGGAGGTGGCGCGTGGGGAAGAACAGGGGTTTTCCTCTGACATTTGGGCGCTTGGGTGTACCTTAATCGAAATGGTCACCGGC

Coding sequence (CDS)

GAACAGGGGATTCTTTCTACTCTGTTCCACCCATCCGTTGTGGGCTACAGGGGATTCGATTTAACGAGAGAGAACGGCAAGGTAATGTATAATCTCTTCATGGAGTACGTCGCCGGCGGTTCCCTCGGCGACGAAATCGTCCGGCGTGGCGGTCGGATTAAGGAGGCGGCGGCGGGGTTCTACACGCGTCAAATTGTCAGGGGATTGGAGTATTTGCACGCGCGAGGATTGGTGCATTGCGATATTAAAGCTAGGAATATTTTGGTCGGTGAAGATGGGGTGAAGATTGCTGATTTCGGCTGCTCCAAATGGGTCTCTCAGCCGGCGGCGATCGGCGGGACACCGATGTTCATGGCGCCGGAGGTGGCGCGTGGGGAAGAACAGGGGTTTTCCTCTGACATTTGGGCGCTTGGGTGTACCTTAATCGAAATGGTCACCGGC

Protein sequence

EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGFYTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAPEVARGEEQGFSSDIWALGCTLIEMVTG

Homology

[Close]

BLAST of MS022377 vs. NCBI nr
Match: XP_022135898.1 (mitogen-activated protein kinase kinase kinase 18-like [Momordica charantia])

HSP 1 Score: 304.3 bits (778), Expect = 5.7e-79
Identity = 146/147 (99.32%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF
Sbjct: 44  EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 103

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP 120
           YT QIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP
Sbjct: 104 YTCQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP 163

Query: 121 EVARGEEQGFSSDIWALGCTLIEMVTG 148
           EVARGEEQGFSSDIWALGCTLIEMVTG
Sbjct: 164 EVARGEEQGFSSDIWALGCTLIEMVTG 190

[Close]

BLAST of MS022377 vs. NCBI nr
Match: XP_022930728.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata])

HSP 1 Score: 263.1 bits (671), Expect = 1.5e-66
Identity = 120/147 (81.63%), Postives = 135/147 (91.84%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQ ILS+L HPS+V YRGFD+TRENGK+MYNLFMEY  GG+L DEI+R GG IKE AAGF
Sbjct: 45  EQRILSSLSHPSIVSYRGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGF 104

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP 120
           YTR+IV+GLEYLH RGLVHCDIKA+NIL+GEDG+KIADFGCSKWV++PAAIGGTPMFMAP
Sbjct: 105 YTREIVKGLEYLHKRGLVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAP 164

Query: 121 EVARGEEQGFSSDIWALGCTLIEMVTG 148
           EVARGE+QG SSDIW+LGCTLIEMV G
Sbjct: 165 EVARGEKQGISSDIWSLGCTLIEMVNG 191

[Close]

BLAST of MS022377 vs. NCBI nr
Match: KAG7022856.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 263.1 bits (671), Expect = 1.5e-66
Identity = 120/147 (81.63%), Postives = 135/147 (91.84%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQ ILS+L HPS+V YRGFD+TRENGK+MYNLFMEY  GG+L DEI+R GG IKE AAGF
Sbjct: 45  EQRILSSLSHPSIVSYRGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGF 104

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP 120
           YTR+IV+GLEYLH RGLVHCDIKA+NIL+GEDG+KIADFGCSKWV++PAAIGGTPMFMAP
Sbjct: 105 YTREIVKGLEYLHKRGLVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAP 164

Query: 121 EVARGEEQGFSSDIWALGCTLIEMVTG 148
           EVARGE+QG SSDIW+LGCTLIEMV G
Sbjct: 165 EVARGEKQGISSDIWSLGCTLIEMVNG 191

[Close]

BLAST of MS022377 vs. NCBI nr
Match: XP_023531740.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 263.1 bits (671), Expect = 1.5e-66
Identity = 120/147 (81.63%), Postives = 135/147 (91.84%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQ ILS+L HPS+V YRGFD+TRENGK+MYNLFMEY  GG+L DEI+R GG IKE AAGF
Sbjct: 45  EQRILSSLSHPSIVSYRGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGF 104

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP 120
           YTR+IV+GLEYLH RGLVHCDIKA+NIL+GEDG+KIADFGCSKWV++PAAIGGTPMFMAP
Sbjct: 105 YTREIVKGLEYLHKRGLVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAP 164

Query: 121 EVARGEEQGFSSDIWALGCTLIEMVTG 148
           EVARGE+QG SSDIW+LGCTLIEMV G
Sbjct: 165 EVARGEKQGISSDIWSLGCTLIEMVNG 191

[Close]

BLAST of MS022377 vs. NCBI nr
Match: KAG6589157.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 261.9 bits (668), Expect = 3.3e-66
Identity = 119/147 (80.95%), Postives = 135/147 (91.84%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQ ILS+L HPS+V YRGFD+TRENG++MYNLFMEY  GG+L DEI+R GG IKE AAGF
Sbjct: 45  EQRILSSLSHPSIVSYRGFDVTRENGRLMYNLFMEYATGGTLADEILRCGGWIKETAAGF 104

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP 120
           YTR+IV+GLEYLH RGLVHCDIKA+NIL+GEDG+KIADFGCSKWV++PAAIGGTPMFMAP
Sbjct: 105 YTREIVKGLEYLHKRGLVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAP 164

Query: 121 EVARGEEQGFSSDIWALGCTLIEMVTG 148
           EVARGE+QG SSDIW+LGCTLIEMV G
Sbjct: 165 EVARGEKQGISSDIWSLGCTLIEMVNG 191

[Close]

BLAST of MS022377 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 4.0e-43
Identity = 86/151 (56.95%), Postives = 108/151 (71.52%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRE-NGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAG 60
           E  ILS+L  P V+GYRG +  RE NG VMYNL MEY   G+L D   + GGR+ E    
Sbjct: 45  EAKILSSLSSPYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVV 104

Query: 61  FYTRQIVRGLEYLHARGLVHCDIKARNILVGEDG-VKIADFGCSKWVSQ--PAAIGGTPM 120
            YTR I++GLEY+H++G+VHCD+K  N+++ E G  KIADFGC+K V     + + GTP 
Sbjct: 105 KYTRDILKGLEYIHSKGIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPA 164

Query: 121 FMAPEVARGEEQGFSSDIWALGCTLIEMVTG 148
           FMAPEVARGE+QG  SDIWA+GCT+IEMVTG
Sbjct: 165 FMAPEVARGEKQGKESDIWAVGCTMIEMVTG 195

[Close]

BLAST of MS022377 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.2e-41
Identity = 89/156 (57.05%), Postives = 108/156 (69.23%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRE----NGK-VMYNLFMEYVAGGSLGDEIVRRGGRIKE 60
           E  ILS+L  P V+GYRG ++TRE    NG+   Y+L MEY   G+L D   + GG I E
Sbjct: 45  EAKILSSLNSPYVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDE 104

Query: 61  AAAGFYTRQIVRGLEYLH-ARGLVHCDIKARNILVGEDG-VKIADFGCSKWVSQPAA--I 120
           A    YTRQI+ GLEY+H ++G+ HCDIK  N+LVGE+G  KIADFGC+KWV       +
Sbjct: 105 ARVVKYTRQILLGLEYIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPV 164

Query: 121 GGTPMFMAPEVARGEEQGFSSDIWALGCTLIEMVTG 148
            GTP FMAPE ARGE QG  SDIWA+GCT+IEMVTG
Sbjct: 165 RGTPAFMAPEAARGERQGKESDIWAVGCTVIEMVTG 200

[Close]

BLAST of MS022377 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.7e-28
Identity = 65/157 (41.40%), Postives = 95/157 (60.51%), Query Frame = 0


Query: 1   EQGILSTLFH-PSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGR-IKEAAA 60
           E+ +L +L   P ++   G D T ENG+ M+NL +EY + GSL   + + GG  + E+  
Sbjct: 49  EKSVLDSLGDCPEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTV 108

Query: 61  GFYTRQIVRGLEYLHARGLVHCDIKARNILVGEDG-VKIADFGCSKWVS-------QPAA 120
             +T  ++RGL ++HA+G  HCDIK  NIL+  DG VKIADFG +  V        +   
Sbjct: 109 RRHTGSVLRGLRHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVE 168

Query: 121 IGGTPMFMAPEVARGEEQGFSSDIWALGCTLIEMVTG 148
           I GTP++MAPE     E G ++D+WALGC ++EM +G
Sbjct: 169 IRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSG 205

[Close]

BLAST of MS022377 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 6.2e-28
Identity = 68/154 (44.16%), Postives = 95/154 (61.69%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           E  +L  L HP++V Y G    RE G +  N+ +E+V GGS+   ++ + G   E+    
Sbjct: 133 EVNLLKNLSHPNIVRYLG--TAREAGSL--NILLEFVPGGSI-SSLLGKFGSFPESVIRM 192

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDG-VKIADFGCSKWVSQPA------AIGG 120
           YT+Q++ GLEYLH  G++H DIK  NILV   G +K+ADFG SK V + A      ++ G
Sbjct: 193 YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKG 252

Query: 121 TPMFMAPEVARGEEQGFSSDIWALGCTLIEMVTG 148
           TP +MAPEV       FS+DIW++GCT+IEM TG
Sbjct: 253 TPYWMAPEVILQTGHSFSADIWSVGCTIIEMATG 281

[Close]

BLAST of MS022377 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 1.8e-27
Identity = 68/154 (44.16%), Postives = 96/154 (62.34%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           E  +L  L HP++V Y G    RE+  +  N+ +E+V GGS+   ++ + G   E+    
Sbjct: 122 EVKLLKNLSHPNIVRYLG--TVREDDTL--NILLEFVPGGSI-SSLLEKFGPFPESVVRT 181

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDG-VKIADFGCSKWVSQPA------AIGG 120
           YTRQ++ GLEYLH   ++H DIK  NILV   G +K+ADFG SK V++ A      ++ G
Sbjct: 182 YTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKG 241

Query: 121 TPMFMAPEVARGEEQGFSSDIWALGCTLIEMVTG 148
           TP +MAPEV       FS+DIW++GCT+IEMVTG
Sbjct: 242 TPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTG 270

[Close]

BLAST of MS022377 vs. ExPASy TrEMBL
Match: A0A6J1C655 (mitogen-activated protein kinase kinase kinase 18-like OS=Momordica charantia OX=3673 GN=LOC111007735 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.8e-79
Identity = 146/147 (99.32%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF
Sbjct: 44  EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 103

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP 120
           YT QIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP
Sbjct: 104 YTCQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP 163

Query: 121 EVARGEEQGFSSDIWALGCTLIEMVTG 148
           EVARGEEQGFSSDIWALGCTLIEMVTG
Sbjct: 164 EVARGEEQGFSSDIWALGCTLIEMVTG 190

[Close]

BLAST of MS022377 vs. ExPASy TrEMBL
Match: A0A6J1EW68 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111437119 PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 7.1e-67
Identity = 120/147 (81.63%), Postives = 135/147 (91.84%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQ ILS+L HPS+V YRGFD+TRENGK+MYNLFMEY  GG+L DEI+R GG IKE AAGF
Sbjct: 45  EQRILSSLSHPSIVSYRGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGF 104

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP 120
           YTR+IV+GLEYLH RGLVHCDIKA+NIL+GEDG+KIADFGCSKWV++PAAIGGTPMFMAP
Sbjct: 105 YTREIVKGLEYLHKRGLVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAP 164

Query: 121 EVARGEEQGFSSDIWALGCTLIEMVTG 148
           EVARGE+QG SSDIW+LGCTLIEMV G
Sbjct: 165 EVARGEKQGISSDIWSLGCTLIEMVNG 191

[Close]

BLAST of MS022377 vs. ExPASy TrEMBL
Match: A0A6J1GLZ4 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111455196 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.6e-66
Identity = 122/148 (82.43%), Postives = 133/148 (89.86%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQ ILS+L HPS+VGY+GFD+T ENGK+MYNLFMEY AGGSL DEIVRRGGRIKE  A F
Sbjct: 45  EQRILSSLSHPSIVGYKGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAF 104

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQ-PAAIGGTPMFMA 120
           YTRQIVRGLEYLH RGLVHCD+KARNIL+G DG+KIADFGCSKWV + PAA+ GTPMFMA
Sbjct: 105 YTRQIVRGLEYLHKRGLVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMA 164

Query: 121 PEVARGEEQGFSSDIWALGCTLIEMVTG 148
           PEVARGE QG SSDIWALGCTLIEMV+G
Sbjct: 165 PEVARGETQGISSDIWALGCTLIEMVSG 192

[Close]

BLAST of MS022377 vs. ExPASy TrEMBL
Match: A0A6J1JKQ2 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111486056 PE=3 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.0e-66
Identity = 119/147 (80.95%), Postives = 133/147 (90.48%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQ ILS+L HPS+V YRGFD+TRENGK+MYNLFMEY  GG+L DEI+R  G IKE AAGF
Sbjct: 45  EQRILSSLSHPSIVSYRGFDVTRENGKLMYNLFMEYATGGTLADEILRCDGWIKETAAGF 104

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWVSQPAAIGGTPMFMAP 120
           YTRQIV+GLEYLH RGLVHCDIKA+NIL+GEDG+KI DFGCSKWV++PAAIGGTPMFMAP
Sbjct: 105 YTRQIVKGLEYLHKRGLVHCDIKAKNILIGEDGLKITDFGCSKWVNEPAAIGGTPMFMAP 164

Query: 121 EVARGEEQGFSSDIWALGCTLIEMVTG 148
           EVARGE+QG SSDIW+LGCTLIEMV G
Sbjct: 165 EVARGEKQGISSDIWSLGCTLIEMVNG 191

[Close]

BLAST of MS022377 vs. ExPASy TrEMBL
Match: A0A6J1HZ76 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111468247 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.9e-64
Identity = 120/148 (81.08%), Postives = 131/148 (88.51%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQ ILS+L HPS+VGY+GFD+T ENGK+MYNLFMEY A GSL DEIVRRGGRIKE  A F
Sbjct: 45  EQRILSSLSHPSIVGYKGFDVTTENGKLMYNLFMEYAACGSLADEIVRRGGRIKEVTAAF 104

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWV-SQPAAIGGTPMFMA 120
           YTRQIVRGLEYLH RGLVHCD+KA+NIL+G DG+KIADFGCSKWV   PAA+ GTPMFMA
Sbjct: 105 YTRQIVRGLEYLHKRGLVHCDVKAKNILIGGDGLKIADFGCSKWVLESPAAVCGTPMFMA 164

Query: 121 PEVARGEEQGFSSDIWALGCTLIEMVTG 148
           PEVARGE QG SSDIWALGCTLIEMV+G
Sbjct: 165 PEVARGETQGISSDIWALGCTLIEMVSG 192

[Close]

BLAST of MS022377 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 180.3 bits (456), Expect = 1.2e-45
Identity = 88/148 (59.46%), Postives = 110/148 (74.32%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQ ILSTL  P +V Y G  LTRE+  ++YN+ MEYV+GG+L D I   GG++ E     
Sbjct: 46  EQSILSTLSSPHMVKYIGTGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRS 105

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGV-KIADFGCSKWVSQPAAIGGTPMFMA 120
           YTRQI+ GL YLH RG+VHCD+K+ N+LV E+GV KIAD GC+K V + +   GTP FMA
Sbjct: 106 YTRQILNGLVYLHERGIVHCDLKSHNVLVEENGVLKIADMGCAKSVDK-SEFSGTPAFMA 165

Query: 121 PEVARGEEQGFSSDIWALGCTLIEMVTG 148
           PEVARGEEQ F +D+WALGCT+IEM+TG
Sbjct: 166 PEVARGEEQRFPADVWALGCTMIEMMTG 192

[Close]

BLAST of MS022377 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 179.5 bits (454), Expect = 2.0e-45
Identity = 83/149 (55.70%), Postives = 109/149 (73.15%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAGF 60
           EQ ILS L  P +V Y G ++T+EN K+MYNL MEYV+GGSL D I   GG++ E     
Sbjct: 47  EQSILSKLSSPYIVKYIGSNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRS 106

Query: 61  YTRQIVRGLEYLHARGLVHCDIKARNILVGEDGVKIADFGCSKWV--SQPAAIGGTPMFM 120
           YTRQI++GL YLH +G+VHCD+K++N+++G +  KI D GC+K V  ++     GTP FM
Sbjct: 107 YTRQILKGLMYLHDQGIVHCDVKSQNVMIGGEIAKIVDLGCAKTVEENENLEFSGTPAFM 166

Query: 121 APEVARGEEQGFSSDIWALGCTLIEMVTG 148
           +PEVARGEEQ F +D+WALGCT+IEM TG
Sbjct: 167 SPEVARGEEQSFPADVWALGCTVIEMATG 195

[Close]

BLAST of MS022377 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 175.6 bits (444), Expect = 2.9e-44
Identity = 86/151 (56.95%), Postives = 108/151 (71.52%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRE-NGKVMYNLFMEYVAGGSLGDEIVRRGGRIKEAAAG 60
           E  ILS+L  P V+GYRG +  RE NG VMYNL MEY   G+L D   + GGR+ E    
Sbjct: 45  EAKILSSLSSPYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVV 104

Query: 61  FYTRQIVRGLEYLHARGLVHCDIKARNILVGEDG-VKIADFGCSKWVSQ--PAAIGGTPM 120
            YTR I++GLEY+H++G+VHCD+K  N+++ E G  KIADFGC+K V     + + GTP 
Sbjct: 105 KYTRDILKGLEYIHSKGIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPA 164

Query: 121 FMAPEVARGEEQGFSSDIWALGCTLIEMVTG 148
           FMAPEVARGE+QG  SDIWA+GCT+IEMVTG
Sbjct: 165 FMAPEVARGEKQGKESDIWAVGCTMIEMVTG 195

[Close]

BLAST of MS022377 vs. TAIR 10
Match: AT1G05100.1 (mitogen-activated protein kinase kinase kinase 18 )

HSP 1 Score: 169.9 bits (429), Expect = 1.6e-42
Identity = 89/156 (57.05%), Postives = 108/156 (69.23%), Query Frame = 0


Query: 1   EQGILSTLFHPSVVGYRGFDLTRE----NGK-VMYNLFMEYVAGGSLGDEIVRRGGRIKE 60
           E  ILS+L  P V+GYRG ++TRE    NG+   Y+L MEY   G+L D   + GG I E
Sbjct: 45  EAKILSSLNSPYVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDE 104

Query: 61  AAAGFYTRQIVRGLEYLH-ARGLVHCDIKARNILVGEDG-VKIADFGCSKWVSQPAA--I 120
           A    YTRQI+ GLEY+H ++G+ HCDIK  N+LVGE+G  KIADFGC+KWV       +
Sbjct: 105 ARVVKYTRQILLGLEYIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPV 164

Query: 121 GGTPMFMAPEVARGEEQGFSSDIWALGCTLIEMVTG 148
            GTP FMAPE ARGE QG  SDIWA+GCT+IEMVTG
Sbjct: 165 RGTPAFMAPEAARGERQGKESDIWAVGCTVIEMVTG 200

[Close]

BLAST of MS022377 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 125.9 bits (315), Expect = 2.6e-29
Identity = 65/157 (41.40%), Postives = 95/157 (60.51%), Query Frame = 0


Query: 1   EQGILSTLFH-PSVVGYRGFDLTRENGKVMYNLFMEYVAGGSLGDEIVRRGGR-IKEAAA 60
           E+ +L +L   P ++   G D T ENG+ M+NL +EY + GSL   + + GG  + E+  
Sbjct: 49  EKSVLDSLGDCPEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTV 108

Query: 61  GFYTRQIVRGLEYLHARGLVHCDIKARNILVGEDG-VKIADFGCSKWVS-------QPAA 120
             +T  ++RGL ++HA+G  HCDIK  NIL+  DG VKIADFG +  V        +   
Sbjct: 109 RRHTGSVLRGLRHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVE 168

Query: 121 IGGTPMFMAPEVARGEEQGFSSDIWALGCTLIEMVTG 148
           I GTP++MAPE     E G ++D+WALGC ++EM +G
Sbjct: 169 IRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSG 205

The following BLAST results are available for this feature:

BLAST of MS022377 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135898.1	5.7e-79	99.32	mitogen-activated protein kinase kinase kinase 18-like [Momordica charantia]	[more]
XP_022930728.1	1.5e-66	81.63	mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata]	[more]
KAG7022856.1	1.5e-66	81.63	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]
XP_023531740.1	1.5e-66	81.63	mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pe...	[more]
KAG6589157.1	3.3e-66	80.95	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]

BLAST of MS022377 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80888	4.0e-43	56.95	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9ZVP5	2.2e-41	57.05	Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370...	[more]
Q9SND6	3.7e-28	41.40	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
Q40541	6.2e-28	44.16	Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097...	[more]
O22040	1.8e-27	44.16	Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of MS022377 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C655	2.8e-79	99.32	mitogen-activated protein kinase kinase kinase 18-like OS=Momordica charantia OX...	[more]
A0A6J1EW68	7.1e-67	81.63	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=...	[more]
A0A6J1GLZ4	4.6e-66	82.43	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=...	[more]
A0A6J1JKQ2	6.0e-66	80.95	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1HZ76	1.9e-64	81.08	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36...	[more]

BLAST of MS022377 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G26890.1	1.2e-45	59.46	mitogen-activated protein kinase kinase kinase 16	[more]
AT5G55090.1	2.0e-45	55.70	mitogen-activated protein kinase kinase kinase 15	[more]
AT2G32510.1	2.9e-44	56.95	mitogen-activated protein kinase kinase kinase 17	[more]
AT1G05100.1	1.6e-42	57.05	mitogen-activated protein kinase kinase kinase 18	[more]
AT3G50310.1	2.6e-29	41.40	mitogen-activated protein kinase kinase kinase 20	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PRINTS	PR00109	TYRKINASE	coord: 34..47 score: 32.87 coord: 71..89 score: 49.16 coord: 132..147 score: 29.08
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 1..147 e-value: 1.5E-10 score: 16.3
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 1..147 e-value: 5.0E-37 score: 127.7
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..147 score: 32.018471
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 1..147 e-value: 6.9E-46 score: 158.7
None	No IPR available	PANTHER	PTHR48011:SF6	MAP KINASE KINASE KINASE	coord: 1..147
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 1..147
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 77..89
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 2..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS022377.1	MS022377.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity