MS022341 (gene) Bitter gourd (TR) v1

Overview


Name	MS022341
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold47: 2663643 .. 2663880 (-)
RNA-Seq Expression	MS022341
Synteny	MS022341

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGTGCCTGTGCCCGATTTCCAACTCATTAATGGCGGACCTAGTTGTGGTCTCCTCTGGCCACACCTTCGACCGCCTCTGAGTCCAAGTATGAGCTTGAAGATGGGTCAGCCCCATATCTCTCCTCCGTTATTACCAACATAAACATAAGATTCACCATTCTCAAGTGGCGCGATAACAACGGAATCGAACACCTGCGAGAGCCCGATTACAGGTCCATTGAACACCGGATAAAGCGA

mRNA sequence

GAGTGCCTGTGCCCGATTTCCAACTCATTAATGGCGGACCTAGTTGTGGTCTCCTCTGGCCACACCTTCGACGCCTCTGAGTCCAAGTATGAGCTTGAAGATGGGTCAGCCCCATATCTCTCCTCCGTTATTACCAACATAAACATAAGATTCACCATTCTCAAGTGGCGCGATAACAACGGAATCGAACACCTGCGAGAGCCCGATTACAGGTCCATTGAACACCGGATAAAGCGA

Coding sequence (CDS)

GAGTGCCTGTGCCCGATTTCCAACTCATTAATGGCGGACCTAGTTGTGGTCTCCTCTGGCCACACCTTCGACGCCTCTGAGTCCAAGTATGAGCTTGAAGATGGGTCAGCCCCATATCTCTCCTCCGTTATTACCAACATAAACATAAGATTCACCATTCTCAAGTGGCGCGATAACAACGGAATCGAACACCTGCGAGAGCCCGATTACAGGTCCATTGAACACCGGATAAAGCGA

Protein sequence

ECLCPISNSLMADLVVVSSGHTFDASESKYELEDGSAPYLSSVITNINIRFTILKWRDNNGIEHLREPDYRSIEHRIKR

Homology

[Close]

BLAST of MS022341 vs. NCBI nr
Match: XP_038895164.1 (U-box domain-containing protein 38-like [Benincasa hispida])

HSP 1 Score: 86.7 bits (213), Expect = 1.0e-13
Identity = 51/83 (61.45%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTF---------DASESKYELEDGSAPYLSSVITNINIRF 60
           E LC IS SLMAD VVVSSG TF         D   S    EDGS P  SSVITN N+R 
Sbjct: 33  EYLCAISKSLMADPVVVSSGQTFERLSVQVCQDLGFSPELEEDGSRPDFSSVITNRNMRS 92

Query: 61  TILKWRDNNGIEHLREPDYRSIE 75
           TILKW DNNGIEH + PDY SIE
Sbjct: 93  TILKWCDNNGIEHPQPPDYSSIE 115

[Close]

BLAST of MS022341 vs. NCBI nr
Match: KAA0055206.1 (U-box domain-containing protein 38-like [Cucumis melo var. makuwa])

HSP 1 Score: 85.9 bits (211), Expect = 1.7e-13
Identity = 51/83 (61.45%), Postives = 52/83 (62.65%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTF---------DASESKYELEDGSAPYLSSVITNINIRF 60
           E LCPIS SLMAD VVVSSG TF         D   S    ED S P  SSVITN NIR 
Sbjct: 33  EYLCPISKSLMADPVVVSSGQTFERLSVKVCQDLGFSPMLEEDDSRPDFSSVITNRNIRS 92

Query: 61  TILKWRDNNGIEHLREPDYRSIE 75
           TILKW DNNGIEH + P Y SIE
Sbjct: 93  TILKWCDNNGIEHPQPPPYTSIE 115

[Close]

BLAST of MS022341 vs. NCBI nr
Match: XP_008438058.1 (PREDICTED: U-box domain-containing protein 38-like [Cucumis melo] >TYJ99136.1 U-box domain-containing protein 38-like [Cucumis melo var. makuwa])

HSP 1 Score: 83.6 bits (205), Expect = 8.5e-13
Identity = 50/83 (60.24%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTF---------DASESKYELEDGSAPYLSSVITNINIRF 60
           E LCPIS SLMAD VVVSSG TF         D   S    ED S P  SSVI N NIR 
Sbjct: 33  EYLCPISKSLMADPVVVSSGQTFERLSVQVCQDLGFSPMLEEDDSRPDFSSVIINRNIRS 92

Query: 61  TILKWRDNNGIEHLREPDYRSIE 75
           TILKW DNNGIEH + P Y SIE
Sbjct: 93  TILKWCDNNGIEHPQPPPYTSIE 115

[Close]

BLAST of MS022341 vs. NCBI nr
Match: XP_023519818.1 (U-box domain-containing protein 38-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 83.2 bits (204), Expect = 1.1e-12
Identity = 49/82 (59.76%), Postives = 55/82 (67.07%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTFD------ASESKY--ELEDGSAPYLSSVITNINIRFT 60
           E LC IS SLMAD VVVSSG TF+        E  +  +LEDGS    +SVITN N+RFT
Sbjct: 33  EYLCEISKSLMADPVVVSSGQTFERLCVQVCQELGFSPKLEDGSRLDCTSVITNRNMRFT 92

Query: 61  ILKWRDNNGIEHLREPDYRSIE 75
           ILKW D+NGIEH R P Y SIE
Sbjct: 93  ILKWCDDNGIEHPRPPQYTSIE 114

[Close]

BLAST of MS022341 vs. NCBI nr
Match: XP_004152547.1 (U-box domain-containing protein 38 [Cucumis sativus] >KGN64288.1 hypothetical protein Csa_013227 [Cucumis sativus])

HSP 1 Score: 82.8 bits (203), Expect = 1.5e-12
Identity = 50/83 (60.24%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTF---------DASESKYELEDGSAPYLSSVITNINIRF 60
           E LCPIS SLMAD VVVSSG TF         D   S    ED S    SSVITN NIR 
Sbjct: 33  EYLCPISKSLMADPVVVSSGQTFERLSVEVCQDLGFSPRLEEDDSRSDFSSVITNRNIRS 92

Query: 61  TILKWRDNNGIEHLREPDYRSIE 75
           TILKW DNNGIEH + P Y SIE
Sbjct: 93  TILKWCDNNGIEHPQPPSYTSIE 115

[Close]

BLAST of MS022341 vs. ExPASy Swiss-Prot
Match: Q9STT1 (U-box domain-containing protein 39 OS=Arabidopsis thaliana OX=3702 GN=PUB39 PE=1 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 2.2e-11
Identity = 37/82 (45.12%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTFDASESKY--------ELEDGSAPYLSSVITNINIRFT 60
           E LCPI+  LM+D VVV+SG TF+    +         +L DG+ P LS+VI N+ ++ T
Sbjct: 35  EFLCPITGFLMSDPVVVASGQTFERISVQVCRNLSFAPKLHDGTQPDLSTVIPNLAMKST 94

Query: 61  ILKWRDNNGIEHLREPDYRSIE 75
           IL W D N +EH R PDY  +E
Sbjct: 95  ILSWCDRNKMEHPRPPDYAYVE 116

[Close]

BLAST of MS022341 vs. ExPASy Swiss-Prot
Match: Q0WUF6 (U-box domain-containing protein 41 OS=Arabidopsis thaliana OX=3702 GN=PUB41 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 3.5e-09
Identity = 34/82 (41.46%), Postives = 47/82 (57.32%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTFDASESKY--------ELEDGSAPYLSSVITNINIRFT 60
           E LCPI+  LM+D VVVSSG TF+    +         +L DG+ P LS+VI N+ ++ T
Sbjct: 34  EFLCPITGFLMSDPVVVSSGQTFERLSVQVCRNLGYIPDLLDGTRPDLSTVIPNLAMKST 93

Query: 61  ILKWRDNNGIEHLREPDYRSIE 75
           I  W D   ++H R PD   +E
Sbjct: 94  IFSWCDRQKVDHPRPPDAAYVE 115

[Close]

BLAST of MS022341 vs. ExPASy Swiss-Prot
Match: Q9FJP6 (U-box domain-containing protein 38 OS=Arabidopsis thaliana OX=3702 GN=PUB38 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.3e-08
Identity = 33/89 (37.08%), Postives = 50/89 (56.18%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTFDA----------SESKYELEDGSAPYLSSVITNINIR 60
           E LCPIS S+M+D VVVSSG TF+              K   ++ S P  S++I N+N++
Sbjct: 36  EFLCPISKSVMSDPVVVSSGQTFERVCVQVCRDLNFIPKLNDDEESLPDFSNIIPNLNMK 95

Query: 61  FTILKWRDNNGIEHLREPDYRSIEHRIKR 80
            TI  W D  G+   + PDY ++E  +++
Sbjct: 96  STIDTWCDTVGVSRPQPPDYSTVERILRQ 124

[Close]

BLAST of MS022341 vs. ExPASy Swiss-Prot
Match: Q9ZV31 (U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 3.4e-04
Identity = 29/77 (37.66%), Postives = 40/77 (51.95%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTFDASESKYELEDG--SAPYLSSVIT------NINIRFT 60
           E  CPIS  LM D V+VSSG T++    K  LE G  + P     +T      N  +R  
Sbjct: 259 EFRCPISLELMTDPVIVSSGQTYERECIKKWLEGGHLTCPKTQETLTSDIMTPNYVLRSL 318

Query: 61  ILKWRDNNGIEHLREPD 70
           I +W ++NGIE  + P+
Sbjct: 319 IAQWCESNGIEPPKRPN 335

[Close]

BLAST of MS022341 vs. ExPASy TrEMBL
Match: A0A5A7ULP5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G00470 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 8.3e-14
Identity = 51/83 (61.45%), Postives = 52/83 (62.65%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTF---------DASESKYELEDGSAPYLSSVITNINIRF 60
           E LCPIS SLMAD VVVSSG TF         D   S    ED S P  SSVITN NIR 
Sbjct: 33  EYLCPISKSLMADPVVVSSGQTFERLSVKVCQDLGFSPMLEEDDSRPDFSSVITNRNIRS 92

Query: 61  TILKWRDNNGIEHLREPDYRSIE 75
           TILKW DNNGIEH + P Y SIE
Sbjct: 93  TILKWCDNNGIEHPQPPPYTSIE 115

[Close]

BLAST of MS022341 vs. ExPASy TrEMBL
Match: A0A5D3BJ60 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003420 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 4.1e-13
Identity = 50/83 (60.24%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTF---------DASESKYELEDGSAPYLSSVITNINIRF 60
           E LCPIS SLMAD VVVSSG TF         D   S    ED S P  SSVI N NIR 
Sbjct: 33  EYLCPISKSLMADPVVVSSGQTFERLSVQVCQDLGFSPMLEEDDSRPDFSSVIINRNIRS 92

Query: 61  TILKWRDNNGIEHLREPDYRSIE 75
           TILKW DNNGIEH + P Y SIE
Sbjct: 93  TILKWCDNNGIEHPQPPPYTSIE 115

[Close]

BLAST of MS022341 vs. ExPASy TrEMBL
Match: A0A1S3AW21 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483277 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 4.1e-13
Identity = 50/83 (60.24%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTF---------DASESKYELEDGSAPYLSSVITNINIRF 60
           E LCPIS SLMAD VVVSSG TF         D   S    ED S P  SSVI N NIR 
Sbjct: 33  EYLCPISKSLMADPVVVSSGQTFERLSVQVCQDLGFSPMLEEDDSRPDFSSVIINRNIRS 92

Query: 61  TILKWRDNNGIEHLREPDYRSIE 75
           TILKW DNNGIEH + P Y SIE
Sbjct: 93  TILKWCDNNGIEHPQPPPYTSIE 115

[Close]

BLAST of MS022341 vs. ExPASy TrEMBL
Match: A0A0A0LR64 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G045860 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 7.0e-13
Identity = 50/83 (60.24%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTF---------DASESKYELEDGSAPYLSSVITNINIRF 60
           E LCPIS SLMAD VVVSSG TF         D   S    ED S    SSVITN NIR 
Sbjct: 33  EYLCPISKSLMADPVVVSSGQTFERLSVEVCQDLGFSPRLEEDDSRSDFSSVITNRNIRS 92

Query: 61  TILKWRDNNGIEHLREPDYRSIE 75
           TILKW DNNGIEH + P Y SIE
Sbjct: 93  TILKWCDNNGIEHPQPPSYTSIE 115

[Close]

BLAST of MS022341 vs. ExPASy TrEMBL
Match: A0A6J1E734 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111431320 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-12
Identity = 49/82 (59.76%), Postives = 55/82 (67.07%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTFD------ASESKY--ELEDGSAPYLSSVITNINIRFT 60
           E LC IS SLMAD VVVSSG TF+        E  +  +LEDGS    +SVITN N+RFT
Sbjct: 33  EYLCEISKSLMADPVVVSSGQTFERLCIQVCQELGFSPKLEDGSRLDFTSVITNRNMRFT 92

Query: 61  ILKWRDNNGIEHLREPDYRSIE 75
           ILKW D+NGIEH R P Y SIE
Sbjct: 93  ILKWCDDNGIEHPRPPLYTSIE 114

[Close]

BLAST of MS022341 vs. TAIR 10
Match: AT3G47820.1 (PLANT U-BOX 39 )

HSP 1 Score: 69.3 bits (168), Expect = 1.6e-12
Identity = 37/82 (45.12%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 1  ECLCPISNSLMADLVVVSSGHTFDASESKY--------ELEDGSAPYLSSVITNINIRFT 60
          E LCPI+  LM+D VVV+SG TF+    +         +L DG+ P LS+VI N+ ++ T
Sbjct: 13 EFLCPITGFLMSDPVVVASGQTFERISVQVCRNLSFAPKLHDGTQPDLSTVIPNLAMKST 72

Query: 61 ILKWRDNNGIEHLREPDYRSIE 75
          IL W D N +EH R PDY  +E
Sbjct: 73 ILSWCDRNKMEHPRPPDYAYVE 94

[Close]

BLAST of MS022341 vs. TAIR 10
Match: AT5G62560.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 62.0 bits (149), Expect = 2.5e-10
Identity = 34/82 (41.46%), Postives = 47/82 (57.32%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTFDASESKY--------ELEDGSAPYLSSVITNINIRFT 60
           E LCPI+  LM+D VVVSSG TF+    +         +L DG+ P LS+VI N+ ++ T
Sbjct: 34  EFLCPITGFLMSDPVVVSSGQTFERLSVQVCRNLGYIPDLLDGTRPDLSTVIPNLAMKST 93

Query: 61  ILKWRDNNGIEHLREPDYRSIE 75
           I  W D   ++H R PD   +E
Sbjct: 94  IFSWCDRQKVDHPRPPDAAYVE 115

[Close]

BLAST of MS022341 vs. TAIR 10
Match: AT5G65200.1 (plant U-box 38 )

HSP 1 Score: 59.3 bits (142), Expect = 1.6e-09
Identity = 33/89 (37.08%), Postives = 50/89 (56.18%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTFDA----------SESKYELEDGSAPYLSSVITNINIR 60
           E LCPIS S+M+D VVVSSG TF+              K   ++ S P  S++I N+N++
Sbjct: 36  EFLCPISKSVMSDPVVVSSGQTFERVCVQVCRDLNFIPKLNDDEESLPDFSNIIPNLNMK 95

Query: 61  FTILKWRDNNGIEHLREPDYRSIEHRIKR 80
            TI  W D  G+   + PDY ++E  +++
Sbjct: 96  STIDTWCDTVGVSRPQPPDYSTVERILRQ 124

[Close]

BLAST of MS022341 vs. TAIR 10
Match: AT2G28830.1 (PLANT U-BOX 12 )

HSP 1 Score: 45.4 bits (106), Expect = 2.4e-05
Identity = 29/77 (37.66%), Postives = 40/77 (51.95%), Query Frame = 0


Query: 1   ECLCPISNSLMADLVVVSSGHTFDASESKYELEDG--SAPYLSSVIT------NINIRFT 60
           E  CPIS  LM D V+VSSG T++    K  LE G  + P     +T      N  +R  
Sbjct: 259 EFRCPISLELMTDPVIVSSGQTYERECIKKWLEGGHLTCPKTQETLTSDIMTPNYVLRSL 318

Query: 61  ILKWRDNNGIEHLREPD 70
           I +W ++NGIE  + P+
Sbjct: 319 IAQWCESNGIEPPKRPN 335

[Close]

BLAST of MS022341 vs. TAIR 10
Match: AT3G54790.1 (ARM repeat superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 2.0e-04
Identity = 25/67 (37.31%), Postives = 35/67 (52.24%), Query Frame = 0


Query: 4   CPISNSLMADLVVVSSGHTFDASESKYELEDGSA--PYLSSVIT------NINIRFTILK 63
           CP+S  LM D V+V+SG TFD +  K  L++G A  P    V+T      N  ++  I  
Sbjct: 244 CPLSTELMLDPVIVASGQTFDRTSIKKWLDNGLAVCPRTRQVLTHQELIPNYTVKAMIAS 303

The following BLAST results are available for this feature:

BLAST of MS022341 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895164.1	1.0e-13	61.45	U-box domain-containing protein 38-like [Benincasa hispida]	[more]
KAA0055206.1	1.7e-13	61.45	U-box domain-containing protein 38-like [Cucumis melo var. makuwa]	[more]
XP_008438058.1	8.5e-13	60.24	PREDICTED: U-box domain-containing protein 38-like [Cucumis melo] >TYJ99136.1 U-...	[more]
XP_023519818.1	1.1e-12	59.76	U-box domain-containing protein 38-like [Cucurbita pepo subsp. pepo]	[more]
XP_004152547.1	1.5e-12	60.24	U-box domain-containing protein 38 [Cucumis sativus] >KGN64288.1 hypothetical pr...	[more]

BLAST of MS022341 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9STT1	2.2e-11	45.12	U-box domain-containing protein 39 OS=Arabidopsis thaliana OX=3702 GN=PUB39 PE=1...	[more]
Q0WUF6	3.5e-09	41.46	U-box domain-containing protein 41 OS=Arabidopsis thaliana OX=3702 GN=PUB41 PE=2...	[more]
Q9FJP6	2.3e-08	37.08	U-box domain-containing protein 38 OS=Arabidopsis thaliana OX=3702 GN=PUB38 PE=1...	[more]
Q9ZV31	3.4e-04	37.66	U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2...	[more]

BLAST of MS022341 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7ULP5	8.3e-14	61.45	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A5D3BJ60	4.1e-13	60.24	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3AW21	4.1e-13	60.24	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483277 PE=4 ...	[more]
A0A0A0LR64	7.0e-13	60.24	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G045860 PE...	[more]
A0A6J1E734	1.2e-12	59.76	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111431320...	[more]

BLAST of MS022341 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47820.1	1.6e-12	45.12	PLANT U-BOX 39	[more]
AT5G62560.1	2.5e-10	41.46	RING/U-box superfamily protein with ARM repeat domain	[more]
AT5G65200.1	1.6e-09	37.08	plant U-box 38	[more]
AT2G28830.1	2.4e-05	37.66	PLANT U-BOX 12	[more]
AT3G54790.1	2.0e-04	37.31	ARM repeat superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..62 e-value: 8.6E-6 score: 27.5
None	No IPR available	PANTHER	PTHR23315:SF276	U-BOX DOMAIN-CONTAINING PROTEIN 38	coord: 1..78
None	No IPR available	PANTHER	PTHR23315	U BOX DOMAIN-CONTAINING	coord: 1..78
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 2..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS022341.1	MS022341.1	mRNA