MS022277 (gene) Bitter gourd (TR) v1

BLAST of MS022277 vs. NCBI nr
Match: XP_022135592.1 (LOW QUALITY PROTEIN: subtilisin-like protease SBT5.3 [Momordica charantia])

HSP 1 Score: 1449.1 bits (3750), Expect = 0.0e+00
Identity = 740/783 (94.51%), Postives = 747/783 (95.40%), Query Frame = 0

Query: 1   TMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSH 60
           TMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSH
Sbjct: 14  TMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSH 73

Query: 61  HEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLH 120
           HEFLQPFLA SDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLH
Sbjct: 74  HEFLQPFLA-SDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLH 133

Query: 121 TTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDL-------- 180
           TTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTG++  S+      +        
Sbjct: 134 TTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGIYLISRRXVXSSIVVLACLNY 193

Query: 181 GPIPSRWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTL 240
             + S  R        P     RKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTL
Sbjct: 194 AKLISLDRXPXNLCKFPVIXTRRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTL 253

Query: 241 STAGGNFVAGASVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHD 300
           STAGGNFVAGASVFGLGNGT+KGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHD
Sbjct: 254 STAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHD 313

Query: 301 GVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVG 360
           GVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVG
Sbjct: 314 GVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVG 373

Query: 361 ASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAG 420
           ASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAG
Sbjct: 374 ASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAG 433

Query: 421 TLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHI 480
           TLDPMKAKGKILVCLRGDNARVDKGEQAMLAGA GMILANSEASGNEILADPHVLPASHI
Sbjct: 434 TLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHI 493

Query: 481 NFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAP 540
           NFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAP
Sbjct: 494 NFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAP 553

Query: 541 GVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRS 600
           GVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRS
Sbjct: 554 GVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRS 613

Query: 601 AIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCAL 660
           AIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCAL
Sbjct: 614 AIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCAL 673

Query: 661 GYNQTQISQFADGPFNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIR 720
           GYNQTQISQFADGPFNC+EPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIR
Sbjct: 674 GYNQTQISQFADGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIR 733

Query: 721 KPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 776
           KPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV
Sbjct: 734 KPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 793

BLAST of MS022277 vs. NCBI nr
Match: XP_038888965.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 1368.2 bits (3540), Expect = 0.0e+00
Identity = 683/773 (88.36%), Postives = 720/773 (93.14%), Query Frame = 0

Query: 5   TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFL 64
           T SS+ F    LLP FLLLSS LL+PTSAIKRSYVVYMGAHSHGGR+PAD+VA+SHH+FL
Sbjct: 5   TISSATF----LLP-FLLLSSSLLTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHKFL 64

Query: 65  QPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRS 124
           +PFL   D+F EDVI YSYTR+INGFAAMLED+VA +LAKH  VVS+FLN+GRKLHTTRS
Sbjct: 65  RPFL-KRDDFTEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSIFLNKGRKLHTTRS 124

Query: 125 WEFMGLE-KNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSRWRGIC 184
           WEFMG+E  NGVI SESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIP RWRGIC
Sbjct: 125 WEFMGMENNNGVITSESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPLRWRGIC 184

Query: 185 QNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 244
           QNQ+DPSFHCNRKLIGARYFNKGYESVVG LNSSFNSPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 185 QNQNDPSFHCNRKLIGARYFNKGYESVVGPLNSSFNSPRDKEGHGSHTLSTAGGNFVAGA 244

Query: 245 SVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGG 304
           SVFGLGNGT+KGGSPRARVAAYKVCWPPAAGNECFDADILAAFD AIHDGVDVLS+SLGG
Sbjct: 245 SVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDSAIHDGVDVLSVSLGG 304

Query: 305 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSR 364
           DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGP AG+V+NIAPW+ITVGAS+MDRKFPS 
Sbjct: 305 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPAAGTVTNIAPWQITVGASTMDRKFPSL 364

Query: 365 VVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 424
           VVLG+ KQIEGESLS  ALPSR FYPL SAADV+LANAS HEAQLCKAGTLDPMKAKGKI
Sbjct: 365 VVLGNKKQIEGESLSQNALPSRKFYPLVSAADVRLANASAHEAQLCKAGTLDPMKAKGKI 424

Query: 425 LVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 484
           LVCLRGDNARVDKGEQA+LAGAVGMILAN+E SGNEILADPHVLPASHINFTDG AV  Y
Sbjct: 425 LVCLRGDNARVDKGEQALLAGAVGMILANNEPSGNEILADPHVLPASHINFTDGTAVLTY 484

Query: 485 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 544
           INSTK P AYITRATTQLGIRPAPFMAAFSSVGPNTI PEILKPDVTAPG+SVIAAYTEA
Sbjct: 485 INSTKFPKAYITRATTQLGIRPAPFMAAFSSVGPNTIIPEILKPDVTAPGLSVIAAYTEA 544

Query: 545 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 604
           EGPTNQEYD RR+ FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTTA TLDN
Sbjct: 545 EGPTNQEYDNRRLPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTANTLDN 604

Query: 605 NLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFA 664
           N EP+LNASYS ATP NYGAGHVHPNGA DPGL+YDIEV EYLLFLCALGYNQ QISQF+
Sbjct: 605 NFEPLLNASYSVATPLNYGAGHVHPNGATDPGLIYDIEVNEYLLFLCALGYNQAQISQFS 664

Query: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKP 724
            GPFNCSEPI+LTNLNYPSITVP+LSRSIT+TRRLKNVGSPGTYKAQIRKP GISVWVKP
Sbjct: 665 SGPFNCSEPINLTNLNYPSITVPELSRSITITRRLKNVGSPGTYKAQIRKPAGISVWVKP 724

Query: 725 KKLNFTRVGEEQSFKVFMKVEKQNVA-KNYVYGDLIWSDGKHHVRSPIVVKAV 776
           KKLNFTR+GEEQSFKV MKV++QN A KNYVYGDLIWSDGKHHVRSPIVVK V
Sbjct: 725 KKLNFTRLGEEQSFKVLMKVKEQNAAKKNYVYGDLIWSDGKHHVRSPIVVKVV 771

BLAST of MS022277 vs. NCBI nr
Match: TYK03381.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 669/773 (86.55%), Postives = 717/773 (92.76%), Query Frame = 0

Query: 5   TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFL 64
           T SSS F    LL LFLLLSS L +PTSAIKRSYVVYMGAHSHGGR+PAD+VA+SHH+FL
Sbjct: 5   TLSSSIF----LLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 64

Query: 65  QPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRS 124
           QPFL S +EF +DVI YSYTR+INGFAAMLED+VA +LAKH  VVSVFLNRGRKLHTTRS
Sbjct: 65  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 124

Query: 125 WEFMGLE-KNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSRWRGIC 184
           WEFMGLE +NGVI SESIWKKARFGEDTIIGNLDTGVW ESKSFSDD++GPIP RW+GIC
Sbjct: 125 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 184

Query: 185 QNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 244
           QNQ+DPSFHCNRKLIGARYFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 185 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 244

Query: 245 SVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGG 304
           SVFGLG GT+KGGSPRARVAAYKVCWPP AGNECFDADILAAFDLAIHDGVDVLS+SLGG
Sbjct: 245 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 304

Query: 305 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSR 364
           DPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+V+N+APW+ITVGAS+MDRKFPS 
Sbjct: 305 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 364

Query: 365 VVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 424
           VVLG+ KQIEGESLS EALPS+  YPL +AADV+LANAS HEAQLCKAGTLDPMKAKGKI
Sbjct: 365 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 424

Query: 425 LVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 484
           LVCLRGDNARVDKGEQA+LAGA GMILAN+E SGNEILADPHVLPA HIN+TDG AVFAY
Sbjct: 425 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 484

Query: 485 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 544
           INSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNT+TPEILKPD+TAPG+SVIAAYTEA
Sbjct: 485 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 544

Query: 545 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 604
           EGPTNQE+D RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTTA+TLDN
Sbjct: 545 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 604

Query: 605 NLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFA 664
           N EP+LNASY  ATPFNYGAGHVHPNGA DPGLVYDI+V EYL FLCALGY++ QISQF+
Sbjct: 605 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 664

Query: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKP 724
           DGPFNCSEPISLTNLNYPSITVPKLSRSIT+TRRLKNVGSPGTY+A+IRKP GISVWVKP
Sbjct: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 724

Query: 725 KKLNFTRVGEEQSFKVFMKVEKQNVA-KNYVYGDLIWSDGKHHVRSPIVVKAV 776
           KKLNFT++GEE SFKVFMKV+   VA KNYVYGDLIWSDGKHHVRSPIVVK V
Sbjct: 725 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of MS022277 vs. NCBI nr
Match: XP_008462019.1 (PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo])

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 669/773 (86.55%), Postives = 717/773 (92.76%), Query Frame = 0

Query: 5   TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFL 64
           T SSS F    LL LFLLLSS L +PTSAIKRSYVVYMGAHSHGGR+PAD+VA+SHH+FL
Sbjct: 5   TLSSSIF----LLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 64

Query: 65  QPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRS 124
           QPFL S +EF +DVI YSYTR+INGFAAMLED+VA +LAKH  VVSVFLNRGRKLHTTRS
Sbjct: 65  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 124

Query: 125 WEFMGLE-KNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSRWRGIC 184
           WEFMGLE +NGVI SESIWKKARFGEDTIIGNLDTGVW ESKSFSDD++GPIP RW+GIC
Sbjct: 125 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 184

Query: 185 QNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 244
           QNQ+DPSFHCNRKLIGARYFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 185 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 244

Query: 245 SVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGG 304
           SVFGLG GT+KGGSPRARVAAYKVCWPP AGNECFDADILAAFDLAIHDGVDVLS+SLGG
Sbjct: 245 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 304

Query: 305 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSR 364
           DPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+V+N+APW+ITVGAS+MDRKFPS 
Sbjct: 305 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 364

Query: 365 VVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 424
           VVLG+ KQIEGESLS EALPS+  YPL +AADV+LANAS HEAQLCKAGTLDPMKAKGKI
Sbjct: 365 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 424

Query: 425 LVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 484
           LVCLRGDNARVDKGEQA+LAGA GMILAN+E SGNEILADPHVLPA HIN+TDG AVFAY
Sbjct: 425 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 484

Query: 485 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 544
           INSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNT+TPEILKPD+TAPG+SVIAAYTEA
Sbjct: 485 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 544

Query: 545 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 604
           EGPTNQE+D RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTTA+TLDN
Sbjct: 545 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 604

Query: 605 NLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFA 664
           N EP+LNASY  ATPFNYGAGHVHPNGA DPGLVYDI+V EYL FLCALGY++ QISQF+
Sbjct: 605 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 664

Query: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKP 724
           DGPFNCSEPISLTNLNYPSITVPKLSRSIT+TRRLKNVGSPGTY+A+IRKP GISVWVKP
Sbjct: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 724

Query: 725 KKLNFTRVGEEQSFKVFMKVEKQNVA-KNYVYGDLIWSDGKHHVRSPIVVKAV 776
           KKLNFT++GEE SFKVFMKV+   VA KNYVYGDLIWSDGKHHVRSPIVVK V
Sbjct: 725 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of MS022277 vs. NCBI nr
Match: XP_022968976.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1345.9 bits (3482), Expect = 0.0e+00
Identity = 674/780 (86.41%), Postives = 720/780 (92.31%), Query Frame = 0

Query: 1   TMKK-TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANS 60
           TMK+ TY S+ F       L LLLSSLLL+PT+AIKRSYVVYMGAHSHGGR+PADIVA+S
Sbjct: 22  TMKQPTYPSTTF-------LLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPADIVADS 81

Query: 61  HHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKL 120
           H E L+P+L S +EF EDVI YSYTR+INGFAAMLED+VA +LAKH  VVSVF NRGR+L
Sbjct: 82  HRELLRPYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAKHPEVVSVFPNRGRRL 141

Query: 121 HTTRSWEFMGLEKN-GVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSD-DDLGPIPS 180
           HTTRSWEFM LE N GVI SESIWKKARFGEDTIIGNLDTGVWPESKSFSD DDLGPIP 
Sbjct: 142 HTTRSWEFMELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQ 201

Query: 181 RWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGG 240
           RWRGICQNQ+DPSFHCNRKLIGARYFNKGY+SVVGHLNSSFNSPRDNEGHGSHTLSTAGG
Sbjct: 202 RWRGICQNQNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGG 261

Query: 241 NFVAGASVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVL 300
           NFVAGASVFGLG G +KGGSPRARVAAYKVCWPPAAGNECFDADILAAFD+AIHDGVDVL
Sbjct: 262 NFVAGASVFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVL 321

Query: 301 SISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMD 360
           S+SLGGDPNPLFNDSVAIGSFHAV+HGIVVICSAGNSGPTAGSV+NIAPW+ITVGAS+MD
Sbjct: 322 SVSLGGDPNPLFNDSVAIGSFHAVRHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMD 381

Query: 361 RKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPM 420
           RKFPSRVVLG+ KQIEGESL+PE LPSR FYPL SAADV+LANAS HEAQLCKAGTLDP 
Sbjct: 382 RKFPSRVVLGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDPK 441

Query: 421 KAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDG 480
           KAKGKILVCLRGDNARVDKGEQAMLAGAVGMILAN+E SGNEILADPHVLPASHINFTDG
Sbjct: 442 KAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDG 501

Query: 481 VAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVI 540
           VAVFAYINSTK P AYIT ATTQLG RPAPFMAAFSSVGP+TITPEILKPDVTAPG+SVI
Sbjct: 502 VAVFAYINSTKFPQAYITGATTQLGTRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVI 561

Query: 541 AAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTT 600
           AAYTEAEGPTNQEYD RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTT
Sbjct: 562 AAYTEAEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT 621

Query: 601 ATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQT 660
           A+TLDNN +P+LNASYS ATPFNYGAGH+HPN A DPGLVYDI + EYL FLCALGYN+ 
Sbjct: 622 ASTLDNNFKPLLNASYSVATPFNYGAGHIHPNKATDPGLVYDITITEYLSFLCALGYNKA 681

Query: 661 QISQFADGPFNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGI 720
           Q+SQF++GPFNCSEPISLTNLNYPSITVP LSRSIT+TRRLKNVGSPGTYKA+IR+P GI
Sbjct: 682 QLSQFSNGPFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRRPAGI 741

Query: 721 SVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVA--KNYVYGDLIWSDGKHHVRSPIVVKAV 776
           SV VKPKKL+FTR+GEE SFKV MKV++ NV   KNYVYGDLIWSDGKHHVRSPIVV A+
Sbjct: 742 SVSVKPKKLSFTRLGEELSFKVLMKVKEGNVGREKNYVYGDLIWSDGKHHVRSPIVVMAI 794

BLAST of MS022277 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 1.2e-287
Identity = 493/777 (63.45%), Postives = 601/777 (77.35%), Query Frame = 0

Query: 2   MKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGG---REPADIVAN 61
           MK T++   F  LLLL L  + S  +L+  S    SYVVY GAHSH G    +  D V  
Sbjct: 1   MKLTHN---FSFLLLLLLVHMSSKHILA--SKDSSSYVVYFGAHSHVGEITEDAMDRVKE 60

Query: 62  SHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRK 121
           +H++FL  F   S E   D I YSYT++INGFAA L+ D+A +++KH  VVSVF N+  K
Sbjct: 61  THYDFLGSF-TGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALK 120

Query: 122 LHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSR 181
           LHTTRSW+F+GLE N  + S SIW+KARFGEDTII NLDTGVWPESKSF D+ LGPIPSR
Sbjct: 121 LHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSR 180

Query: 182 WRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGN 241
           W+GICQNQ D +FHCNRKLIGARYFNKGY + VGHLNSSF+SPRD +GHGSHTLSTA G+
Sbjct: 181 WKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGD 240

Query: 242 FVAGASVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLS 301
           FV G S+FG GNGT+KGGSPRARVAAYKVCWPP  GNEC+DAD+LAAFD AIHDG DV+S
Sbjct: 241 FVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVIS 300

Query: 302 ISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDR 361
           +SLGG+P   FNDSVAIGSFHA K  IVV+CSAGNSGP   +VSN+APW+ITVGAS+MDR
Sbjct: 301 VSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDR 360

Query: 362 KFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMK 421
           +F S +VLG+ K  +G+SLS  ALP   FYP+ ++ + K  NAS  +AQLCK G+LDP+K
Sbjct: 361 EFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIK 420

Query: 422 AKGKILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGV 481
            KGKILVCLRG N RV+KG    L G +GM+L N+  +GN++LADPHVLPA+ +   D  
Sbjct: 421 TKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSF 480

Query: 482 AVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIA 541
           AV  YI+ TK P A+IT + T LG++PAP MA+FSS GP+ + P+ILKPD+TAPGVSVIA
Sbjct: 481 AVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIA 540

Query: 542 AYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTA 601
           AYT A  PTN+++D RR+LFN++SGTSMSCPH+SGIAGLL+T YP WSPAAIRSAIMTTA
Sbjct: 541 AYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTA 600

Query: 602 TTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQ 661
           T +D+   PI NA+  +ATPF++GAGHV PN A++PGLVYD+ +K+YL FLC+LGYN +Q
Sbjct: 601 TIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQ 660

Query: 662 ISQFADGPFNCSEP-ISLTNLNYPSITVPKLSRS-ITVTRRLKNVGSPGTYKAQIRKPLG 721
           IS F+   F CS P ISL NLNYPSITVP L+ S +TV+R +KNVG P  Y  ++  P G
Sbjct: 661 ISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQG 720

Query: 722 ISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVVK 774
           + V VKP  LNFT+VGE+++FKV +   K NVAK YV+G+L+WSD KH VRSPIVVK
Sbjct: 721 VYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVK 771

BLAST of MS022277 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 3.9e-262
Identity = 458/781 (58.64%), Postives = 581/781 (74.39%), Query Frame = 0

Query: 1   TMKKTYSSSAFHH---LLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPA---D 60
           +M + YSS+ + +   L  L   LLL +L  SP  A+K+SY+VY+G+H+H  +  +   D
Sbjct: 2   SMTRRYSSTQYSNKMSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLD 61

Query: 61  IVANSHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLN 120
            VA+SH  FL  F+  S E  ++ I YSY R+INGFAA+L+++ A ++AKH  VVSVF N
Sbjct: 62  GVAHSHRTFLASFV-GSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPN 121

Query: 121 RGRKLHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGP 180
           +GRKLHTT SW FM L KNGV+   S+W KA +GEDTII NLDTGVWPESKSFSD+  G 
Sbjct: 122 KGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGA 181

Query: 181 IPSRWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVG-HLNSSFNSPRDNEGHGSHTLS 240
           +P+RW+G C         CNRKLIGARYFNKGY +  G   N+S+ + RD++GHGSHTLS
Sbjct: 182 VPARWKGRCHK----DVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLS 241

Query: 241 TAGGNFVAGASVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDG 300
           TA GNFV GA+VFG+GNGT+ GGSP+ARVAAYKVCWPP  G ECFDADILAA + AI DG
Sbjct: 242 TAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDG 301

Query: 301 VDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGA 360
           VDVLS S+GGD     +D +AIGSFHAVK+G+ V+CSAGNSGP +G+VSN+APW ITVGA
Sbjct: 302 VDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGA 361

Query: 361 SSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGT 420
           SSMDR+F + V L + +  +G SLS + LP    Y L SAAD  +AN +V +A LCK G+
Sbjct: 362 SSMDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGS 421

Query: 421 LDPMKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHIN 480
           LDP K KGKILVCLRGDNARVDKG QA  AGA GM+L N +ASGNEI++D HVLPAS I+
Sbjct: 422 LDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQID 481

Query: 481 FTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPG 540
           + DG  +F+Y++STK P  YI   T  L  +PAPFMA+FSS GPNTITP ILKPD+TAPG
Sbjct: 482 YKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPG 541

Query: 541 VSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSA 600
           V++IAA+TEA GPT+ + D RR  FN+ SGTSMSCPH+SG+ GLL+TL+PHWSPAAIRSA
Sbjct: 542 VNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSA 601

Query: 601 IMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALG 660
           IMTT+ T +N  +P+++ S+ +A PF+YG+GHV PN A  PGLVYD+   +YL FLCA+G
Sbjct: 602 IMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVG 661

Query: 661 YNQTQISQFADGP-FNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIR 720
           YN T +  FA+ P + C +  +L + NYPSITVP L+ SITVTR+LKNVG P TY A+ R
Sbjct: 662 YNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFR 721

Query: 721 KPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 774
           +PLG+ V V+PK+L F + GE + F++ ++         YV+G+L W+D  H+VRSPIVV
Sbjct: 722 EPLGVRVSVEPKQLTFNKTGEVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVV 775

BLAST of MS022277 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 711.4 bits (1835), Expect = 1.1e-203
Identity = 395/788 (50.13%), Postives = 517/788 (65.61%), Query Frame = 0

Query: 13  HLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPA---DIVANSHHEFLQPFLA 72
           HL+L   FL   + LL+  +  K+ Y+VYMGAHSHG    +   ++  +SH++ L     
Sbjct: 9   HLILSSFFLF--TFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFG 68

Query: 73  SSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRSWEFMG 132
           S ++  ++ I+YSY R+INGFAA+LE++ A  +AK+ +VVSVFL++  KLHTTRSWEF+G
Sbjct: 69  SREK-AKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLG 128

Query: 133 LEKNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSRWR-GICQNQSD 192
           L + G     S W+K RFGE+TIIGN+DTGVWPES+SFSD   G +PS+WR G+CQ    
Sbjct: 129 LHRRG---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKL 188

Query: 193 PSF---HCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 252
           P      CNRKLIGARY+NK +E+  G L+   ++ RD  GHG+HTLSTAGGNFV GA V
Sbjct: 189 PGSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARV 248

Query: 253 FGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGGD- 312
           F +GNGT+KGGSPRARVAAYKVCW       C+ AD+LAA D AI DGVDV+++S G   
Sbjct: 249 FAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSY 308

Query: 313 ---PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFP 372
                 +F D ++IG+FHA+   I+++ SAGN GPT G+V+N+APW  T+ AS++DR F 
Sbjct: 309 VVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFS 368

Query: 373 SRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKG 432
           S + + +N+ IEG SL    LP    + L  + D KLANA+  +AQLC+ GTLD  K  G
Sbjct: 369 SNLTI-NNQLIEGASLFVN-LPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNG 428

Query: 433 KILVCLR-GDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVL------PASHINF 492
           KI++C R G    V +G +A+ AGA GMIL N   +G  + A+PHV       P    + 
Sbjct: 429 KIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSR 488

Query: 493 TDGVAVFAYINSTKSP-----TAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDV 552
             GV   A I     P     T  ++RA T  G +PAP MA+FSS GPN I P ILKPDV
Sbjct: 489 PHGVKTTA-IGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDV 548

Query: 553 TAPGVSVIAAYTEAEGPTNQEYDTRR-VLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPA 612
           TAPGV+++AAY+E    ++   D RR   FN + GTSMSCPH SGIAGLL+T +P WSPA
Sbjct: 549 TAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPA 608

Query: 613 AIRSAIMTTATTLDNNLEPILNA-SYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLL 672
           AI+SAIMTTATTLDN   PI +A   + A  F YG+GHV P+ AI+PGLVYD+ + +YL 
Sbjct: 609 AIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLN 668

Query: 673 FLCALGYNQTQISQF-ADGPFNCSEPISLTNLNYPSITVPKLS-RSITVTRRLKNVGSPG 732
           FLCA GY+Q  IS    +  F CS   S+ +LNYPSIT+P L  + +T+ R + NVG P 
Sbjct: 669 FLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPS 728

Query: 733 TYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHH 774
           TY    R P G S+ V P  L FT++GE ++FKV ++       + Y +GDL W+DGKH 
Sbjct: 729 TYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHI 787

BLAST of MS022277 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 1.7e-172
Identity = 346/774 (44.70%), Postives = 492/774 (63.57%), Query Frame = 0

Query: 8   SSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFLQPF 67
           SS+F       L L L    +S +S+ + +Y+V+M       + P+    + H  +    
Sbjct: 2   SSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAK----SQMPSSF--DLHSNWYDSS 61

Query: 68  LASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRSWEF 127
           L S  +  E  +LY+Y   I+GF+  L  + A  L     V+SV      +LHTTR+  F
Sbjct: 62  LRSISDSAE--LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLF 121

Query: 128 MGLEKNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSRWRGICQNQS 187
           +GL+++    +  ++ +A    D ++G LDTGVWPESKS+SD+  GPIPS W+G C+  +
Sbjct: 122 LGLDEH----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGT 181

Query: 188 D-PSFHCNRKLIGARYFNKGYESVVGHLNSS--FNSPRDNEGHGSHTLSTAGGNFVAGAS 247
           +  +  CNRKLIGAR+F +GYES +G ++ S    SPRD++GHG+HT STA G+ V GAS
Sbjct: 182 NFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGAS 241

Query: 248 VFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGGD 307
           + G  +GT++G +PRARVA YKVCW       CF +DILAA D AI D V+VLS+SLGG 
Sbjct: 242 LLGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGG 301

Query: 308 PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSRV 367
            +  + D VAIG+F A++ GI+V CSAGN+GP++ S+SN+APW  TVGA ++DR FP+  
Sbjct: 302 MSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALA 361

Query: 368 VLGSNKQIEGESL-SPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 427
           +LG+ K   G SL   EALP +   P   A +   +NA+     LC  GTL P K KGKI
Sbjct: 362 ILGNGKNFTGVSLFKGEALPDK-LLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKI 421

Query: 428 LVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 487
           ++C RG NARV KG+    AG VGMILAN+ A+G E++AD H+LPA+ +    G  +  Y
Sbjct: 422 VMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHY 481

Query: 488 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 547
           + +  +PTA I+   T +G++P+P +AAFSS GPN+ITP ILKPD+ APGV+++AA+T A
Sbjct: 482 VTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGA 541

Query: 548 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 607
            GPT    D+RRV FN +SGTSMSCPHVSG+A LL++++P WSPAAIRSA+MTTA     
Sbjct: 542 AGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYK 601

Query: 608 NLEPILN-ASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQF 667
           + +P+L+ A+   +TPF++GAGHV P  A +PGL+YD+  ++YL FLCAL Y   QI   
Sbjct: 602 DGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSV 661

Query: 668 ADGPFNC--SEPISLTNLNYPSITVPKLS-RSITVTRRLKNVGSPGTYKAQI-RKPLGIS 727
           +   + C  S+  S+ +LNYPS  V      +   TR + +VG  GTY  ++  +  G+ 
Sbjct: 662 SRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVK 721

Query: 728 VWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 773
           + V+P  LNF    E++S+ V   V+    + +  +G + WSDGKH V SP+ +
Sbjct: 722 ISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of MS022277 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 5.0e-169
Identity = 350/795 (44.03%), Postives = 468/795 (58.87%), Query Frame = 0

Query: 16  LLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFLQPFLASSDEFV 75
           L PL  L+   LL+  +  K+ Y+VY G H   G +    +   HH +LQ  +  S+E  
Sbjct: 7   LFPLLFLIP--LLASCAEEKQVYIVYFGEHK--GDKAFHEIEEHHHSYLQS-VKESEEDA 66

Query: 76  EDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRK--LHTTRSWEFMGLEK- 135
              +LYSY   INGFAA L  D A+KL K   VVSVF +  RK   HTTRSWEF+GLE+ 
Sbjct: 67  RASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEE 126

Query: 136 --------------NGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSR 195
                         +   +  +  KKA+ G+  I+G LD+GVWPESKSF+D  +GP+P  
Sbjct: 127 ETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKS 186

Query: 196 WRGICQ-NQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFN----SPRDNEGHGSHTLS 255
           W+GICQ   +  S HCNRK+IGARY+ KGYE   G  N++ N    SPRD +GHGSHT S
Sbjct: 187 WKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTAS 246

Query: 256 TAGGNFVAGASVF-GLGNGTSKGGSPRARVAAYKVCW-----PPAAGNECFDADILAAFD 315
           TA G  V GAS   G   G++ GG+P AR+A YK CW         GN C + D+LAA D
Sbjct: 247 TAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAID 306

Query: 316 LAIHDGVDVLSISLG-GDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAP 375
            AI DGV V+SIS+G  +P P   D +A+G+ HAVK  IVV  SAGNSGP  G++SN+AP
Sbjct: 307 DAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAP 366

Query: 376 WEITVGASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEA 435
           W ITVGAS++DR F   +VLG+   I+ +S++  A     F PL  A++V +   +++E 
Sbjct: 367 WIITVGASTLDRAFVGGLVLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNET 426

Query: 436 QLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHV 495
             C   +L P    GK+++CLRG  +R+ KG +   AG  GMIL N  A+GNE+ +D H 
Sbjct: 427 SQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHF 486

Query: 496 LPASHINFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILK 555
           +P + +  T    +  YI + K+P A+I    T    + AP M  FSS GPN + P ILK
Sbjct: 487 VPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILK 546

Query: 556 PDVTAPGVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWS 615
           PD+TAPG+ ++AA++ A+ P+    D R   +N  SGTSMSCPHV+G   LL+ ++P WS
Sbjct: 547 PDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWS 606

Query: 616 PAAIRSAIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYL 675
            AAIRSA+MTTA   ++  +PI + +   A PF  G+GH  P  A DPGLVYD   + YL
Sbjct: 607 SAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYL 666

Query: 676 LFLCALGYNQTQISQFADGPFNCSEPISL-TNLNYPSITVPKLSRSITVTRRLKNVG--- 735
           L+ C++  N T I    D  F C   I    N NYPSI VP L +++TV R + NVG   
Sbjct: 667 LYGCSV--NITNI----DPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGN 726

Query: 736 SPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAK-----NYVYGDL 773
           S  TY   ++ P GISV   P  L+F R+G++Q FK+ +K  K  V        Y +G  
Sbjct: 727 STSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWF 786

BLAST of MS022277 vs. ExPASy TrEMBL
Match: A0A6J1C351 (LOW QUALITY PROTEIN: subtilisin-like protease SBT5.3 OS=Momordica charantia OX=3673 GN=LOC111007513 PE=3 SV=1)

HSP 1 Score: 1449.1 bits (3750), Expect = 0.0e+00
Identity = 740/783 (94.51%), Postives = 747/783 (95.40%), Query Frame = 0

Query: 1   TMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSH 60
           TMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSH
Sbjct: 14  TMKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSH 73

Query: 61  HEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLH 120
           HEFLQPFLA SDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLH
Sbjct: 74  HEFLQPFLA-SDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLH 133

Query: 121 TTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDL-------- 180
           TTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTG++  S+      +        
Sbjct: 134 TTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGIYLISRRXVXSSIVVLACLNY 193

Query: 181 GPIPSRWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTL 240
             + S  R        P     RKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTL
Sbjct: 194 AKLISLDRXPXNLCKFPVIXTRRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTL 253

Query: 241 STAGGNFVAGASVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHD 300
           STAGGNFVAGASVFGLGNGT+KGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHD
Sbjct: 254 STAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHD 313

Query: 301 GVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVG 360
           GVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVG
Sbjct: 314 GVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVG 373

Query: 361 ASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAG 420
           ASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAG
Sbjct: 374 ASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAG 433

Query: 421 TLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHI 480
           TLDPMKAKGKILVCLRGDNARVDKGEQAMLAGA GMILANSEASGNEILADPHVLPASHI
Sbjct: 434 TLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAAGMILANSEASGNEILADPHVLPASHI 493

Query: 481 NFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAP 540
           NFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAP
Sbjct: 494 NFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAP 553

Query: 541 GVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRS 600
           GVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRS
Sbjct: 554 GVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRS 613

Query: 601 AIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCAL 660
           AIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCAL
Sbjct: 614 AIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCAL 673

Query: 661 GYNQTQISQFADGPFNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIR 720
           GYNQTQISQFADGPFNC+EPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIR
Sbjct: 674 GYNQTQISQFADGPFNCAEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIR 733

Query: 721 KPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 776
           KPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV
Sbjct: 734 KPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 793

BLAST of MS022277 vs. ExPASy TrEMBL
Match: A0A5D3BUG6 (Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84663G00160 PE=3 SV=1)

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 669/773 (86.55%), Postives = 717/773 (92.76%), Query Frame = 0

Query: 5   TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFL 64
           T SSS F    LL LFLLLSS L +PTSAIKRSYVVYMGAHSHGGR+PAD+VA+SHH+FL
Sbjct: 5   TLSSSIF----LLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 64

Query: 65  QPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRS 124
           QPFL S +EF +DVI YSYTR+INGFAAMLED+VA +LAKH  VVSVFLNRGRKLHTTRS
Sbjct: 65  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 124

Query: 125 WEFMGLE-KNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSRWRGIC 184
           WEFMGLE +NGVI SESIWKKARFGEDTIIGNLDTGVW ESKSFSDD++GPIP RW+GIC
Sbjct: 125 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 184

Query: 185 QNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 244
           QNQ+DPSFHCNRKLIGARYFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 185 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 244

Query: 245 SVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGG 304
           SVFGLG GT+KGGSPRARVAAYKVCWPP AGNECFDADILAAFDLAIHDGVDVLS+SLGG
Sbjct: 245 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 304

Query: 305 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSR 364
           DPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+V+N+APW+ITVGAS+MDRKFPS 
Sbjct: 305 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 364

Query: 365 VVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 424
           VVLG+ KQIEGESLS EALPS+  YPL +AADV+LANAS HEAQLCKAGTLDPMKAKGKI
Sbjct: 365 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 424

Query: 425 LVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 484
           LVCLRGDNARVDKGEQA+LAGA GMILAN+E SGNEILADPHVLPA HIN+TDG AVFAY
Sbjct: 425 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 484

Query: 485 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 544
           INSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNT+TPEILKPD+TAPG+SVIAAYTEA
Sbjct: 485 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 544

Query: 545 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 604
           EGPTNQE+D RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTTA+TLDN
Sbjct: 545 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 604

Query: 605 NLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFA 664
           N EP+LNASY  ATPFNYGAGHVHPNGA DPGLVYDI+V EYL FLCALGY++ QISQF+
Sbjct: 605 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 664

Query: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKP 724
           DGPFNCSEPISLTNLNYPSITVPKLSRSIT+TRRLKNVGSPGTY+A+IRKP GISVWVKP
Sbjct: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 724

Query: 725 KKLNFTRVGEEQSFKVFMKVEKQNVA-KNYVYGDLIWSDGKHHVRSPIVVKAV 776
           KKLNFT++GEE SFKVFMKV+   VA KNYVYGDLIWSDGKHHVRSPIVVK V
Sbjct: 725 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of MS022277 vs. ExPASy TrEMBL
Match: A0A1S3CG03 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500491 PE=3 SV=1)

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 669/773 (86.55%), Postives = 717/773 (92.76%), Query Frame = 0

Query: 5   TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFL 64
           T SSS F    LL LFLLLSS L +PTSAIKRSYVVYMGAHSHGGR+PAD+VA+SHH+FL
Sbjct: 5   TLSSSIF----LLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 64

Query: 65  QPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRS 124
           QPFL S +EF +DVI YSYTR+INGFAAMLED+VA +LAKH  VVSVFLNRGRKLHTTRS
Sbjct: 65  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 124

Query: 125 WEFMGLE-KNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSRWRGIC 184
           WEFMGLE +NGVI SESIWKKARFGEDTIIGNLDTGVW ESKSFSDD++GPIP RW+GIC
Sbjct: 125 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 184

Query: 185 QNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 244
           QNQ+DPSFHCNRKLIGARYFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 185 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 244

Query: 245 SVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGG 304
           SVFGLG GT+KGGSPRARVAAYKVCWPP AGNECFDADILAAFDLAIHDGVDVLS+SLGG
Sbjct: 245 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 304

Query: 305 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSR 364
           DPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+V+N+APW+ITVGAS+MDRKFPS 
Sbjct: 305 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 364

Query: 365 VVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 424
           VVLG+ KQIEGESLS EALPS+  YPL +AADV+LANAS HEAQLCKAGTLDPMKAKGKI
Sbjct: 365 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 424

Query: 425 LVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 484
           LVCLRGDNARVDKGEQA+LAGA GMILAN+E SGNEILADPHVLPA HIN+TDG AVFAY
Sbjct: 425 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 484

Query: 485 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 544
           INSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNT+TPEILKPD+TAPG+SVIAAYTEA
Sbjct: 485 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 544

Query: 545 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 604
           EGPTNQE+D RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTTA+TLDN
Sbjct: 545 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 604

Query: 605 NLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFA 664
           N EP+LNASY  ATPFNYGAGHVHPNGA DPGLVYDI+V EYL FLCALGY++ QISQF+
Sbjct: 605 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 664

Query: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKP 724
           DGPFNCSEPISLTNLNYPSITVPKLSRSIT+TRRLKNVGSPGTY+A+IRKP GISVWVKP
Sbjct: 665 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 724

Query: 725 KKLNFTRVGEEQSFKVFMKVEKQNVA-KNYVYGDLIWSDGKHHVRSPIVVKAV 776
           KKLNFT++GEE SFKVFMKV+   VA KNYVYGDLIWSDGKHHVRSPIVVK V
Sbjct: 725 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of MS022277 vs. ExPASy TrEMBL
Match: A0A6J1HWD2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468122 PE=3 SV=1)

HSP 1 Score: 1345.9 bits (3482), Expect = 0.0e+00
Identity = 674/780 (86.41%), Postives = 720/780 (92.31%), Query Frame = 0

Query: 1   TMKK-TYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANS 60
           TMK+ TY S+ F       L LLLSSLLL+PT+AIKRSYVVYMGAHSHGGR+PADIVA+S
Sbjct: 22  TMKQPTYPSTTF-------LLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPADIVADS 81

Query: 61  HHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKL 120
           H E L+P+L S +EF EDVI YSYTR+INGFAAMLED+VA +LAKH  VVSVF NRGR+L
Sbjct: 82  HRELLRPYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAKHPEVVSVFPNRGRRL 141

Query: 121 HTTRSWEFMGLEKN-GVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSD-DDLGPIPS 180
           HTTRSWEFM LE N GVI SESIWKKARFGEDTIIGNLDTGVWPESKSFSD DDLGPIP 
Sbjct: 142 HTTRSWEFMELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQ 201

Query: 181 RWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGG 240
           RWRGICQNQ+DPSFHCNRKLIGARYFNKGY+SVVGHLNSSFNSPRDNEGHGSHTLSTAGG
Sbjct: 202 RWRGICQNQNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGG 261

Query: 241 NFVAGASVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVL 300
           NFVAGASVFGLG G +KGGSPRARVAAYKVCWPPAAGNECFDADILAAFD+AIHDGVDVL
Sbjct: 262 NFVAGASVFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVL 321

Query: 301 SISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMD 360
           S+SLGGDPNPLFNDSVAIGSFHAV+HGIVVICSAGNSGPTAGSV+NIAPW+ITVGAS+MD
Sbjct: 322 SVSLGGDPNPLFNDSVAIGSFHAVRHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMD 381

Query: 361 RKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPM 420
           RKFPSRVVLG+ KQIEGESL+PE LPSR FYPL SAADV+LANAS HEAQLCKAGTLDP 
Sbjct: 382 RKFPSRVVLGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDPK 441

Query: 421 KAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDG 480
           KAKGKILVCLRGDNARVDKGEQAMLAGAVGMILAN+E SGNEILADPHVLPASHINFTDG
Sbjct: 442 KAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDG 501

Query: 481 VAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVI 540
           VAVFAYINSTK P AYIT ATTQLG RPAPFMAAFSSVGP+TITPEILKPDVTAPG+SVI
Sbjct: 502 VAVFAYINSTKFPQAYITGATTQLGTRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVI 561

Query: 541 AAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTT 600
           AAYTEAEGPTNQEYD RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAIMTT
Sbjct: 562 AAYTEAEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT 621

Query: 601 ATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQT 660
           A+TLDNN +P+LNASYS ATPFNYGAGH+HPN A DPGLVYDI + EYL FLCALGYN+ 
Sbjct: 622 ASTLDNNFKPLLNASYSVATPFNYGAGHIHPNKATDPGLVYDITITEYLSFLCALGYNKA 681

Query: 661 QISQFADGPFNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGI 720
           Q+SQF++GPFNCSEPISLTNLNYPSITVP LSRSIT+TRRLKNVGSPGTYKA+IR+P GI
Sbjct: 682 QLSQFSNGPFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRRPAGI 741

Query: 721 SVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVA--KNYVYGDLIWSDGKHHVRSPIVVKAV 776
           SV VKPKKL+FTR+GEE SFKV MKV++ NV   KNYVYGDLIWSDGKHHVRSPIVV A+
Sbjct: 742 SVSVKPKKLSFTRLGEELSFKVLMKVKEGNVGREKNYVYGDLIWSDGKHHVRSPIVVMAI 794

BLAST of MS022277 vs. ExPASy TrEMBL
Match: A0A6J1GL32 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111455314 PE=3 SV=1)

HSP 1 Score: 1335.9 bits (3456), Expect = 0.0e+00
Identity = 665/761 (87.39%), Postives = 710/761 (93.30%), Query Frame = 0

Query: 19  LFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFLQPFLASSDEFVEDV 78
           L LLLSSLLL+PT+AIKRSYVVYMGAHSHGGR+PA+IVA+SH + L+P+L S +EF EDV
Sbjct: 12  LLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVADSHRDLLRPYLESGNEFAEDV 71

Query: 79  ILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRSWEFMGLEKN-GVII 138
           I YSYTR+INGFAAMLED VA +LA+H  VVSVF NRGR+LHTTRSWEFM LE N GVI 
Sbjct: 72  IFYSYTRHINGFAAMLEDQVAARLAQHPEVVSVFPNRGRRLHTTRSWEFMELENNHGVIS 131

Query: 139 SESIWKKARFGEDTIIGNLDTGVWPESKSFSD-DDLGPIPSRWRGICQNQSDPSFHCNRK 198
           SESIWKKARFGEDTIIGNLDTGVWPESKSFSD DDLGPIP RWRGICQNQ+DPSFHCNRK
Sbjct: 132 SESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQRWRGICQNQNDPSFHCNRK 191

Query: 199 LIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGNGTSKGG 258
           LIGARYFNKGY+SVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLG G +KGG
Sbjct: 192 LIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGG 251

Query: 259 SPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGGDPNPLFNDSVAIG 318
           SPRARVAAYKVCWPPAAGNECFDADILAAFD+AIHDGVDVLS+SLGGDPNPLFNDSVAIG
Sbjct: 252 SPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSVAIG 311

Query: 319 SFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSRVVLGSNKQIEGES 378
           SFHAVKHGIVVICSAGNSGPTAGSV+NIAPW+ITVGAS+MDRKFPSRVVLG+ KQIEGES
Sbjct: 312 SFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQIEGES 371

Query: 379 LSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDK 438
           L+PE LPSR FYPL SAA+V+LANAS HEAQLCKAGTLDP KAKGKILVCLRGDNARVDK
Sbjct: 372 LTPEGLPSRKFYPLMSAANVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARVDK 431

Query: 439 GEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGVAVFAYINSTKSPTAYITR 498
           GEQAMLAGAVGMILAN+E SGNEILADPHVLPASHINFTDGVAVFAY+NSTK P AYIT 
Sbjct: 432 GEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYVNSTKFPQAYITG 491

Query: 499 ATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEAEGPTNQEYDTRRV 558
           ATTQLGIRPAPFMAAFSSVGP+TITPEILKPDVTAPG+SVIAAYT AEGPTNQEYD RRV
Sbjct: 492 ATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKRRV 551

Query: 559 LFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDNNLEPILNASYSEA 618
            FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAI+SAI+TTA+TLDNN +P+LNASYS A
Sbjct: 552 AFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIVTTASTLDNNFKPLLNASYSVA 611

Query: 619 TPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQFADGPFNCSEPISLT 678
           TPFNYGAGH+HPN A DPGLVYDI V EYL FLCALGYN+ Q+SQF++GPFNCSEPISLT
Sbjct: 612 TPFNYGAGHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNGPFNCSEPISLT 671

Query: 679 NLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQS 738
           NLNYPSITVP LSRSIT+TRRLKNVGSPGTYKA+IRKP GISV VKPKKL+FTR+GEE S
Sbjct: 672 NLNYPSITVPNLSRSITITRRLKNVGSPGTYKAKIRKPAGISVSVKPKKLSFTRLGEELS 731

Query: 739 FKVFMKVEKQNVA--KNYVYGDLIWSDGKHHVRSPIVVKAV 776
           FKV MKV++ NV   KNYVYGDLIWSDGKHHVRSPIVV AV
Sbjct: 732 FKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 772

BLAST of MS022277 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 990.3 bits (2559), Expect = 8.5e-289
Identity = 493/777 (63.45%), Postives = 601/777 (77.35%), Query Frame = 0

Query: 2   MKKTYSSSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGG---REPADIVAN 61
           MK T++   F  LLLL L  + S  +L+  S    SYVVY GAHSH G    +  D V  
Sbjct: 1   MKLTHN---FSFLLLLLLVHMSSKHILA--SKDSSSYVVYFGAHSHVGEITEDAMDRVKE 60

Query: 62  SHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRK 121
           +H++FL  F   S E   D I YSYT++INGFAA L+ D+A +++KH  VVSVF N+  K
Sbjct: 61  THYDFLGSF-TGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALK 120

Query: 122 LHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSR 181
           LHTTRSW+F+GLE N  + S SIW+KARFGEDTII NLDTGVWPESKSF D+ LGPIPSR
Sbjct: 121 LHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSR 180

Query: 182 WRGICQNQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFNSPRDNEGHGSHTLSTAGGN 241
           W+GICQNQ D +FHCNRKLIGARYFNKGY + VGHLNSSF+SPRD +GHGSHTLSTA G+
Sbjct: 181 WKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGD 240

Query: 242 FVAGASVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLS 301
           FV G S+FG GNGT+KGGSPRARVAAYKVCWPP  GNEC+DAD+LAAFD AIHDG DV+S
Sbjct: 241 FVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVIS 300

Query: 302 ISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDR 361
           +SLGG+P   FNDSVAIGSFHA K  IVV+CSAGNSGP   +VSN+APW+ITVGAS+MDR
Sbjct: 301 VSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDR 360

Query: 362 KFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMK 421
           +F S +VLG+ K  +G+SLS  ALP   FYP+ ++ + K  NAS  +AQLCK G+LDP+K
Sbjct: 361 EFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIK 420

Query: 422 AKGKILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGV 481
            KGKILVCLRG N RV+KG    L G +GM+L N+  +GN++LADPHVLPA+ +   D  
Sbjct: 421 TKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSF 480

Query: 482 AVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIA 541
           AV  YI+ TK P A+IT + T LG++PAP MA+FSS GP+ + P+ILKPD+TAPGVSVIA
Sbjct: 481 AVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIA 540

Query: 542 AYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTA 601
           AYT A  PTN+++D RR+LFN++SGTSMSCPH+SGIAGLL+T YP WSPAAIRSAIMTTA
Sbjct: 541 AYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTA 600

Query: 602 TTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQ 661
           T +D+   PI NA+  +ATPF++GAGHV PN A++PGLVYD+ +K+YL FLC+LGYN +Q
Sbjct: 601 TIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQ 660

Query: 662 ISQFADGPFNCSEP-ISLTNLNYPSITVPKLSRS-ITVTRRLKNVGSPGTYKAQIRKPLG 721
           IS F+   F CS P ISL NLNYPSITVP L+ S +TV+R +KNVG P  Y  ++  P G
Sbjct: 661 ISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQG 720

Query: 722 ISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVVK 774
           + V VKP  LNFT+VGE+++FKV +   K NVAK YV+G+L+WSD KH VRSPIVVK
Sbjct: 721 VYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVK 771

BLAST of MS022277 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 905.6 bits (2339), Expect = 2.8e-263
Identity = 458/781 (58.64%), Postives = 581/781 (74.39%), Query Frame = 0

Query: 1   TMKKTYSSSAFHH---LLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPA---D 60
           +M + YSS+ + +   L  L   LLL +L  SP  A+K+SY+VY+G+H+H  +  +   D
Sbjct: 2   SMTRRYSSTQYSNKMSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLD 61

Query: 61  IVANSHHEFLQPFLASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLN 120
            VA+SH  FL  F+  S E  ++ I YSY R+INGFAA+L+++ A ++AKH  VVSVF N
Sbjct: 62  GVAHSHRTFLASFV-GSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPN 121

Query: 121 RGRKLHTTRSWEFMGLEKNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGP 180
           +GRKLHTT SW FM L KNGV+   S+W KA +GEDTII NLDTGVWPESKSFSD+  G 
Sbjct: 122 KGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGA 181

Query: 181 IPSRWRGICQNQSDPSFHCNRKLIGARYFNKGYESVVG-HLNSSFNSPRDNEGHGSHTLS 240
           +P+RW+G C         CNRKLIGARYFNKGY +  G   N+S+ + RD++GHGSHTLS
Sbjct: 182 VPARWKGRCHK----DVPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLS 241

Query: 241 TAGGNFVAGASVFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDG 300
           TA GNFV GA+VFG+GNGT+ GGSP+ARVAAYKVCWPP  G ECFDADILAA + AI DG
Sbjct: 242 TAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDG 301

Query: 301 VDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGA 360
           VDVLS S+GGD     +D +AIGSFHAVK+G+ V+CSAGNSGP +G+VSN+APW ITVGA
Sbjct: 302 VDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGA 361

Query: 361 SSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGT 420
           SSMDR+F + V L + +  +G SLS + LP    Y L SAAD  +AN +V +A LCK G+
Sbjct: 362 SSMDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGS 421

Query: 421 LDPMKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHIN 480
           LDP K KGKILVCLRGDNARVDKG QA  AGA GM+L N +ASGNEI++D HVLPAS I+
Sbjct: 422 LDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQID 481

Query: 481 FTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPG 540
           + DG  +F+Y++STK P  YI   T  L  +PAPFMA+FSS GPNTITP ILKPD+TAPG
Sbjct: 482 YKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPG 541

Query: 541 VSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSA 600
           V++IAA+TEA GPT+ + D RR  FN+ SGTSMSCPH+SG+ GLL+TL+PHWSPAAIRSA
Sbjct: 542 VNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSA 601

Query: 601 IMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALG 660
           IMTT+ T +N  +P+++ S+ +A PF+YG+GHV PN A  PGLVYD+   +YL FLCA+G
Sbjct: 602 IMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVG 661

Query: 661 YNQTQISQFADGP-FNCSEPISLTNLNYPSITVPKLSRSITVTRRLKNVGSPGTYKAQIR 720
           YN T +  FA+ P + C +  +L + NYPSITVP L+ SITVTR+LKNVG P TY A+ R
Sbjct: 662 YNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFR 721

Query: 721 KPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 774
           +PLG+ V V+PK+L F + GE + F++ ++         YV+G+L W+D  H+VRSPIVV
Sbjct: 722 EPLGVRVSVEPKQLTFNKTGEVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVV 775

BLAST of MS022277 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 607.8 bits (1566), Expect = 1.2e-173
Identity = 346/774 (44.70%), Postives = 492/774 (63.57%), Query Frame = 0

Query: 8   SSAFHHLLLLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFLQPF 67
           SS+F       L L L    +S +S+ + +Y+V+M       + P+    + H  +    
Sbjct: 2   SSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAK----SQMPSSF--DLHSNWYDSS 61

Query: 68  LASSDEFVEDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRSWEF 127
           L S  +  E  +LY+Y   I+GF+  L  + A  L     V+SV      +LHTTR+  F
Sbjct: 62  LRSISDSAE--LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLF 121

Query: 128 MGLEKNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSRWRGICQNQS 187
           +GL+++    +  ++ +A    D ++G LDTGVWPESKS+SD+  GPIPS W+G C+  +
Sbjct: 122 LGLDEH----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGT 181

Query: 188 D-PSFHCNRKLIGARYFNKGYESVVGHLNSS--FNSPRDNEGHGSHTLSTAGGNFVAGAS 247
           +  +  CNRKLIGAR+F +GYES +G ++ S    SPRD++GHG+HT STA G+ V GAS
Sbjct: 182 NFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGAS 241

Query: 248 VFGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGGD 307
           + G  +GT++G +PRARVA YKVCW       CF +DILAA D AI D V+VLS+SLGG 
Sbjct: 242 LLGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGG 301

Query: 308 PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPSRV 367
            +  + D VAIG+F A++ GI+V CSAGN+GP++ S+SN+APW  TVGA ++DR FP+  
Sbjct: 302 MSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALA 361

Query: 368 VLGSNKQIEGESL-SPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGKI 427
           +LG+ K   G SL   EALP +   P   A +   +NA+     LC  GTL P K KGKI
Sbjct: 362 ILGNGKNFTGVSLFKGEALPDK-LLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKI 421

Query: 428 LVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGVAVFAY 487
           ++C RG NARV KG+    AG VGMILAN+ A+G E++AD H+LPA+ +    G  +  Y
Sbjct: 422 VMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHY 481

Query: 488 INSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTEA 547
           + +  +PTA I+   T +G++P+P +AAFSS GPN+ITP ILKPD+ APGV+++AA+T A
Sbjct: 482 VTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGA 541

Query: 548 EGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLDN 607
            GPT    D+RRV FN +SGTSMSCPHVSG+A LL++++P WSPAAIRSA+MTTA     
Sbjct: 542 AGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYK 601

Query: 608 NLEPILN-ASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQF 667
           + +P+L+ A+   +TPF++GAGHV P  A +PGL+YD+  ++YL FLCAL Y   QI   
Sbjct: 602 DGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSV 661

Query: 668 ADGPFNC--SEPISLTNLNYPSITVPKLS-RSITVTRRLKNVGSPGTYKAQI-RKPLGIS 727
           +   + C  S+  S+ +LNYPS  V      +   TR + +VG  GTY  ++  +  G+ 
Sbjct: 662 SRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVK 721

Query: 728 VWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAKNYVYGDLIWSDGKHHVRSPIVV 773
           + V+P  LNF    E++S+ V   V+    + +  +G + WSDGKH V SP+ +
Sbjct: 722 ISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of MS022277 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 596.3 bits (1536), Expect = 3.6e-170
Identity = 350/795 (44.03%), Postives = 468/795 (58.87%), Query Frame = 0

Query: 16  LLPLFLLLSSLLLSPTSAIKRSYVVYMGAHSHGGREPADIVANSHHEFLQPFLASSDEFV 75
           L PL  L+   LL+  +  K+ Y+VY G H   G +    +   HH +LQ  +  S+E  
Sbjct: 7   LFPLLFLIP--LLASCAEEKQVYIVYFGEHK--GDKAFHEIEEHHHSYLQS-VKESEEDA 66

Query: 76  EDVILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRK--LHTTRSWEFMGLEK- 135
              +LYSY   INGFAA L  D A+KL K   VVSVF +  RK   HTTRSWEF+GLE+ 
Sbjct: 67  RASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEE 126

Query: 136 --------------NGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSR 195
                         +   +  +  KKA+ G+  I+G LD+GVWPESKSF+D  +GP+P  
Sbjct: 127 ETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKS 186

Query: 196 WRGICQ-NQSDPSFHCNRKLIGARYFNKGYESVVGHLNSSFN----SPRDNEGHGSHTLS 255
           W+GICQ   +  S HCNRK+IGARY+ KGYE   G  N++ N    SPRD +GHGSHT S
Sbjct: 187 WKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTAS 246

Query: 256 TAGGNFVAGASVF-GLGNGTSKGGSPRARVAAYKVCW-----PPAAGNECFDADILAAFD 315
           TA G  V GAS   G   G++ GG+P AR+A YK CW         GN C + D+LAA D
Sbjct: 247 TAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAID 306

Query: 316 LAIHDGVDVLSISLG-GDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAP 375
            AI DGV V+SIS+G  +P P   D +A+G+ HAVK  IVV  SAGNSGP  G++SN+AP
Sbjct: 307 DAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAP 366

Query: 376 WEITVGASSMDRKFPSRVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEA 435
           W ITVGAS++DR F   +VLG+   I+ +S++  A     F PL  A++V +   +++E 
Sbjct: 367 WIITVGASTLDRAFVGGLVLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNET 426

Query: 436 QLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHV 495
             C   +L P    GK+++CLRG  +R+ KG +   AG  GMIL N  A+GNE+ +D H 
Sbjct: 427 SQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHF 486

Query: 496 LPASHINFTDGVAVFAYINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILK 555
           +P + +  T    +  YI + K+P A+I    T    + AP M  FSS GPN + P ILK
Sbjct: 487 VPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILK 546

Query: 556 PDVTAPGVSVIAAYTEAEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWS 615
           PD+TAPG+ ++AA++ A+ P+    D R   +N  SGTSMSCPHV+G   LL+ ++P WS
Sbjct: 547 PDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWS 606

Query: 616 PAAIRSAIMTTATTLDNNLEPILNASYSEATPFNYGAGHVHPNGAIDPGLVYDIEVKEYL 675
            AAIRSA+MTTA   ++  +PI + +   A PF  G+GH  P  A DPGLVYD   + YL
Sbjct: 607 SAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYL 666

Query: 676 LFLCALGYNQTQISQFADGPFNCSEPISL-TNLNYPSITVPKLSRSITVTRRLKNVG--- 735
           L+ C++  N T I    D  F C   I    N NYPSI VP L +++TV R + NVG   
Sbjct: 667 LYGCSV--NITNI----DPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGN 726

Query: 736 SPGTYKAQIRKPLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVAK-----NYVYGDL 773
           S  TY   ++ P GISV   P  L+F R+G++Q FK+ +K  K  V        Y +G  
Sbjct: 727 STSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWF 786

BLAST of MS022277 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 590.1 bits (1520), Expect = 2.6e-168
Identity = 329/723 (45.50%), Postives = 461/723 (63.76%), Query Frame = 0

Query: 71  SDEFVEDV-ILYSYTRYINGFAAMLEDDVATKLAKHRSVVSVFLNRGRKLHTTRSWEFMG 130
           S EF E+  I++ Y    +GF+A++  D A  L  H +V++VF +R R+LHTTRS +F+G
Sbjct: 49  STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLG 108

Query: 131 LEKNGVIISESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPSRWRGICQNQSDP 190
           L+       + +W ++ +G D IIG  DTG+WPE +SFSD +LGPIP RWRG+C++ +  
Sbjct: 109 LQN-----QKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARF 168

Query: 191 S-FHCNRKLIGARYFNKGYE-SVVGHLNSS--FNSPRDNEGHGSHTLSTAGGNFVAGASV 250
           S  +CNRK+IGAR+F KG + +V+G +N +  F SPRD +GHG+HT STA G     AS+
Sbjct: 169 SPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASM 228

Query: 251 FGLGNGTSKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDLAIHDGVDVLSISLGGD- 310
            G  +G +KG +P+AR+AAYKVCW  +    C D+DILAAFD A+ DGVDV+SIS+GG  
Sbjct: 229 SGYASGVAKGVAPKARIAAYKVCWKDSG---CLDSDILAAFDAAVRDGVDVISISIGGGD 288

Query: 311 --PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVSNIAPWEITVGASSMDRKFPS 370
              +P + D +AIGS+ A   GI V  SAGN GP   SV+N+APW  TVGAS++DR FP+
Sbjct: 289 GITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPA 348

Query: 371 RVVLGSNKQIEGESLSPEALPSRNFYPLTSAADVKLANASVHEAQLCKAGTLDPMKAKGK 430
             +LG   ++ G SL      +   +P+     V    + +  A LC   TLDP + +GK
Sbjct: 349 DAILGDGHRLRGVSLYAGVPLNGRMFPV-----VYPGKSGMSSASLCMENTLDPKQVRGK 408

Query: 431 ILVCLRGDNARVDKGEQAMLAGAVGMILANSEASGNEILADPHVLPASHINFTDGVAVFA 490
           I++C RG + RV KG     AG VGMILAN  ++G  ++ D H++PA  +   +G  + A
Sbjct: 409 IVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKA 468

Query: 491 YINSTKSPTAYITRATTQLGIRPAPFMAAFSSVGPNTITPEILKPDVTAPGVSVIAAYTE 550
           Y +S  +P A I    T +GI+PAP +A+FS  GPN ++PEILKPD+ APGV+++AA+T+
Sbjct: 469 YASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTD 528

Query: 551 AEGPTNQEYDTRRVLFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIRSAIMTTATTLD 610
           A GPT    D R+  FN +SGTSM+CPHVSG A LL++ +P WSPA IRSA+MTT   +D
Sbjct: 529 AVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVD 588

Query: 611 NNLEPILNASYSE-ATPFNYGAGHVHPNGAIDPGLVYDIEVKEYLLFLCALGYNQTQISQ 670
           N+   +++ S  + ATP++YG+GH++   A++PGLVYDI   +Y+ FLC++GY    I  
Sbjct: 589 NSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQV 648

Query: 671 FADGPFNC--SEPISLTNLNYPSITV--PKLSRSI---TVTRRLKNVG-SPGTYKAQIRK 730
               P  C  +   S  NLNYPSIT   P   R +   TV R   NVG +   Y+A+I  
Sbjct: 649 ITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIES 708

Query: 731 PLGISVWVKPKKLNFTRVGEEQSFKVFMKVEKQNVA---KNYVYGDLIWSD-GKHHVRSP 773
           P G++V VKP +L FT   + +S+ V + V  +NV       V+G + W D GKH VRSP
Sbjct: 709 PRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSP 758