MS022253 (gene) Bitter gourd (TR) v1

BLAST of MS022253 vs. NCBI nr
Match: XP_022136208.1 (uncharacterized protein LOC111007960 [Momordica charantia] >XP_022136209.1 uncharacterized protein LOC111007960 [Momordica charantia] >XP_022136210.1 uncharacterized protein LOC111007960 [Momordica charantia])

HSP 1 Score: 1216.8 bits (3147), Expect = 0.0e+00
Identity = 611/615 (99.35%), Postives = 612/615 (99.51%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA
Sbjct: 27  RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 86

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
           GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN
Sbjct: 87  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 146

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNFRRRCSVTLTSSSPSYHDAASQVLYRLGDLPTQ 180
           VLRRKSQDCHQLEVVKKLSQLLNDPNNFRRRCSVTLTSSSPSYHDAASQVLYRLGDLPTQ
Sbjct: 147 VLRRKSQDCHQLEVVKKLSQLLNDPNNFRRRCSVTLTSSSPSYHDAASQVLYRLGDLPTQ 206

Query: 181 GLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLAKAMA 240
           GLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLAKAMA
Sbjct: 207 GLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLAKAMA 266

Query: 241 VADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSLLDPD 300
           VADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSLLDPD
Sbjct: 267 VADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSLLDPD 326

Query: 301 AKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEEIEKE 360
           AKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEEIEKE
Sbjct: 327 AKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEEIEKE 386

Query: 361 VECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCDGLPLDDNG 420
           VECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCDGLPLDDNG
Sbjct: 387 VECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCDGLPLDDNG 446

Query: 421 SHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHAFTGECPLDSSFKYCPSFMESKFQKD 480
           SHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHAFTGECPLDSSFKYCPSFMESKFQKD
Sbjct: 447 SHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHAFTGECPLDSSFKYCPSFMESKFQKD 506

Query: 481 THHRDDSPSSKFTPNILLDKSTSDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQE 540
           THHRDDSPSSKFTPNILLDKS+SDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQE
Sbjct: 507 THHRDDSPSSKFTPNILLDKSSSDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQE 566

Query: 541 ACDETSLIAYNFIGRLLEEFAKSEGVELDWCANLYLTSVSSTEEDLP--EDEQTHIKGNG 600
           ACDETSLIAYN IGRLLEEFAKSEGVELDWCANLYLTSVSSTEEDLP  EDEQTHIKGNG
Sbjct: 567 ACDETSLIAYNVIGRLLEEFAKSEGVELDWCANLYLTSVSSTEEDLPESEDEQTHIKGNG 626

Query: 601 NFIQVCKELIPSLSK 614
           NFIQVCKELIPSLSK
Sbjct: 627 NFIQVCKELIPSLSK 641

BLAST of MS022253 vs. NCBI nr
Match: XP_023529626.1 (uncharacterized protein LOC111792415 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529627.1 uncharacterized protein LOC111792415 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529628.1 uncharacterized protein LOC111792415 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 906.0 bits (2340), Expect = 1.8e-259
Identity = 483/656 (73.63%), Postives = 531/656 (80.95%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLGLPTS    K  DHSDFLNK+NLPE LLREDDVFYETVKTRVEEAFGALNVET   
Sbjct: 27  RWLLGLPTSVPSLKHFDHSDFLNKKNLPESLLREDDVFYETVKTRVEEAFGALNVETSRL 86

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
           GI  D++ DTCK+ KLILSCL+DLST GLYLLA+I T+DSV+LEKTR KLKRV++EFIP 
Sbjct: 87  GIPVDQMLDTCKVRKLILSCLNDLSTNGLYLLAVIFTEDSVRLEKTRCKLKRVIKEFIPK 146

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNF----RRRCSVTLTSSSPSYHDAASQVLYRLGD 180
           VLRR+SQDCHQLE+VK+L+QLLNDP  F    RR C+ T TSS PS  DAASQVLYRLGD
Sbjct: 147 VLRRESQDCHQLEIVKRLAQLLNDPKTFRRGCRRGCAATSTSSLPSLQDAASQVLYRLGD 206

Query: 181 LPTQGLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLA 240
           LPTQGL+AM RKL GVRVMPQ+KRH+HGWGRDRLIN+LT+ S KMLSS  EGD+LQESLA
Sbjct: 207 LPTQGLIAMHRKLSGVRVMPQMKRHKHGWGRDRLINVLTKISKKMLSSLREGDKLQESLA 266

Query: 241 KAMAVADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSL 300
           KAMA+A+LSLKLV G HNSS IEFYPF PQIKTLHNEIVKAIW VRKK +F+KLKQLKSL
Sbjct: 267 KAMAMAELSLKLVSGHHNSSIIEFYPFSPQIKTLHNEIVKAIWFVRKKSDFRKLKQLKSL 326

Query: 301 LDPDAKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEE 360
           LDPD+KV NRCLRTAIK+MLIDYLFEC DMD VPKSLLKALA+INADSR+AS+SFFS +E
Sbjct: 327 LDPDSKVPNRCLRTAIKQMLIDYLFECCDMDAVPKSLLKALAMINADSRSASHSFFSQDE 386

Query: 361 IEKEVECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCD-GLP 420
            E+EVECVFSLSAQMKQVVWDLLPN +FEH+F DAYMEELEESDDDFDD+ + +CD GLP
Sbjct: 387 TEEEVECVFSLSAQMKQVVWDLLPNREFEHEFVDAYMEELEESDDDFDDDGSGSCDGGLP 446

Query: 421 LDDNGSHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHA-----------------FTG 480
            DDNGSHS     +VEGMGESMP NL +SSVGN LSPS A                 FT 
Sbjct: 447 RDDNGSHS----FYVEGMGESMPGNLGYSSVGNVLSPSQASMKSPDVRPLQCSELTHFTR 506

Query: 481 ECPLDSSFKYCPSFMESKFQKDT------------------HHRDDSPSSKFTPNILLDK 540
           E  LDSSF +C SFMESK Q DT                  HH DDSPSSKFTPNILLDK
Sbjct: 507 EGSLDSSFNFCSSFMESKVQIDTYNLSSNQQVGNKDTISFSHHIDDSPSSKFTPNILLDK 566

Query: 541 STSDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQEACDETSLIAYNFIGRLLEEF 600
           ST D A T CLSS NMSCAMPQM PSKPS FKNQYL+IQEACD TS+IAYNFIGRLLEEF
Sbjct: 567 STIDLAKTPCLSSFNMSCAMPQMEPSKPSPFKNQYLMIQEACDGTSMIAYNFIGRLLEEF 626

Query: 601 AKSEGVELDWCANLYLTSVSSTEEDLPEDEQTHIKGNGN---FIQVCKELIPSLSK 614
           AKSEGV+LDWCANLYL++  S EEDLPE EQTH K   +    IQVC+ELIPSLS+
Sbjct: 627 AKSEGVDLDWCANLYLSTHCSIEEDLPEVEQTHTKAKRSDSVIIQVCEELIPSLSE 678

BLAST of MS022253 vs. NCBI nr
Match: KAG7022776.1 (hypothetical protein SDJN02_16512, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 900.6 bits (2326), Expect = 7.5e-258
Identity = 482/657 (73.36%), Postives = 530/657 (80.67%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLGLPTS    K  DHSDFLNK+NLPE LLREDDVFYETVKTRVEEAFGALNVET   
Sbjct: 40  RWLLGLPTSVPSLKHFDHSDFLNKKNLPESLLREDDVFYETVKTRVEEAFGALNVETSRL 99

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
           GI  D++ DTCK+ KLILSCL+DLST GLYLLAII T+DSV LEKTR KLKRV++EFIP 
Sbjct: 100 GIPVDQMLDTCKVRKLILSCLNDLSTNGLYLLAIIFTEDSVGLEKTRCKLKRVIKEFIPK 159

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNF----RRRCSVTLTSSSPSYHDAASQVLYRLGD 180
           VLRR+SQDCHQLE+VK+L+QLLNDP NF    RR CS T TSS PS  DAASQVLYRLGD
Sbjct: 160 VLRRESQDCHQLEIVKRLAQLLNDPKNFRRGCRRGCSATSTSSLPSLQDAASQVLYRLGD 219

Query: 181 LPTQGLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLA 240
           LPTQGL+AM RKL GVRVMPQ+KRH+HGWGRDRLIN+LT+ S KMLSS  EGD+LQESLA
Sbjct: 220 LPTQGLIAMHRKLSGVRVMPQMKRHKHGWGRDRLINVLTKISKKMLSSLREGDKLQESLA 279

Query: 241 KAMAVADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSL 300
           KAMA+A+LSLKLV G HNSS IEFYPF PQIKTLHNEIVKAIW VRKK +F+KLKQLKSL
Sbjct: 280 KAMAMAELSLKLVSGHHNSSIIEFYPFSPQIKTLHNEIVKAIWFVRKKSDFRKLKQLKSL 339

Query: 301 LDPDAKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEE 360
           LDPD+KV NRCLRTAIK+MLIDYLFEC DMDTVPKSLLKALA+INADSR+AS+SFFS +E
Sbjct: 340 LDPDSKVPNRCLRTAIKQMLIDYLFECCDMDTVPKSLLKALAMINADSRSASHSFFSQDE 399

Query: 361 IEKEVECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCD-GLP 420
            E+EVEC+FSLSAQMKQVVWDLLPN +FEH+FADAYMEELEESDDDFDD+ + +CD GLP
Sbjct: 400 TEEEVECLFSLSAQMKQVVWDLLPNREFEHEFADAYMEELEESDDDFDDDGSGSCDGGLP 459

Query: 421 LDDNGSHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHA-----------------FTG 480
            +DNGSHS     +VEGMGESMP NL +SSVGN LSPS A                 FT 
Sbjct: 460 REDNGSHSV----YVEGMGESMPGNLGYSSVGNVLSPSQASMKSPDVRPPQRSELTHFTR 519

Query: 481 ECPLDSSFKYCPSFMESKFQKDT------------------HHRDDSPSSKFTPNILLDK 540
           E  LD SF +C SFMESK Q DT                  H  +DSPSSKFTP+ILLDK
Sbjct: 520 EGSLDCSFNFCSSFMESKVQIDTYNLSSNQQVVNKDTISFSHRMNDSPSSKFTPSILLDK 579

Query: 541 STSDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQEACDETSLIAYNFIGRLLEEF 600
           ST D A T CLSS NMSCAMPQM P KP+ FKNQYLVIQEACD TS+IAYNFIGRLLEEF
Sbjct: 580 STIDLAKTPCLSSFNMSCAMPQMEPCKPNPFKNQYLVIQEACDGTSMIAYNFIGRLLEEF 639

Query: 601 AKSEGVELDWCANLYLTSVSSTEEDLPEDEQTHIKGNGN---FIQVCKELIPSLSKR 615
           AKSEGV+LDWCANLYL++ SS EED PE EQTH K  G     I VC+ELIPSLS+R
Sbjct: 640 AKSEGVDLDWCANLYLSTHSSIEEDPPEVEQTHTKAKGGDSVIIHVCEELIPSLSER 692

BLAST of MS022253 vs. NCBI nr
Match: XP_038890245.1 (uncharacterized protein LOC120079870 isoform X1 [Benincasa hispida] >XP_038890246.1 uncharacterized protein LOC120079870 isoform X1 [Benincasa hispida])

HSP 1 Score: 900.2 bits (2325), Expect = 9.8e-258
Identity = 480/636 (75.47%), Postives = 527/636 (82.86%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLGLPTS S  K SDHSDFLNKRNLPE LLREDDVFYETVKTRVEEAFG LNVETRH 
Sbjct: 27  RWLLGLPTSVSRPKYSDHSDFLNKRNLPESLLREDDVFYETVKTRVEEAFGVLNVETRHL 86

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
           GI+ +RI DTCK+GKLI+SCL+DLS RGLYLLAIILT+DSV+LEKTRWKLKR ++EFIP 
Sbjct: 87  GIRANRILDTCKVGKLIMSCLNDLSIRGLYLLAIILTEDSVQLEKTRWKLKRAIKEFIPK 146

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNFRRRCSVTLTSSSPSYHDAASQVLYRLGDLPTQ 180
           VLRRKS+DC QLE+VK LSQL ND  NFRRRCS TLTSSS S HDA SQVLY LGDLPTQ
Sbjct: 147 VLRRKSEDCRQLEMVKGLSQLFNDSKNFRRRCSATLTSSSLSIHDAVSQVLYGLGDLPTQ 206

Query: 181 GLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLAKAMA 240
            LLAMRRKLEGVR MPQ+KRHRHGWGRDRLIN+LT+ S KMLSS GEGDELQESLAKAMA
Sbjct: 207 VLLAMRRKLEGVRFMPQMKRHRHGWGRDRLINLLTKISEKMLSSIGEGDELQESLAKAMA 266

Query: 241 VADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSLLDPD 300
           VADLS KLVPG HNSS IEFYPF PQIKTLHNEIVKAIW VR+KGNFQKLKQLKSLLDPD
Sbjct: 267 VADLSHKLVPGRHNSSLIEFYPFSPQIKTLHNEIVKAIWFVRRKGNFQKLKQLKSLLDPD 326

Query: 301 AKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEEIEKE 360
           AKVS+R LR +IK MLIDYLFECSDMDTVPKSLLKALA++NADSR+A++S FS +EIE++
Sbjct: 327 AKVSHRNLRHSIKAMLIDYLFECSDMDTVPKSLLKALALVNADSRSATDSVFSQDEIEED 386

Query: 361 VECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCDGLPLDDNG 420
            ECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDF++ ++D+CDG P +D  
Sbjct: 387 AECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFEEINDDSCDGWPQEDKE 446

Query: 421 SHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHAFTG-----------------ECPLD 480
             S     +VEGMGESMPANL+HSSVGN L+PS A                    E  LD
Sbjct: 447 PQSV----YVEGMGESMPANLDHSSVGNILAPSQASLNNADVESLQYSTPMHVKREGSLD 506

Query: 481 SSFKYCPSFMESKFQKDTHH--RDDSPSSKFTPNILLDK-STSDTATTACLSSLNMSCAM 540
           SSF    SFMESK Q D  +   +    ++ TPNILLDK STS T+ T CLSS NMS  M
Sbjct: 507 SSFSCRLSFMESKGQHDACNLSSNQQVGNEGTPNILLDKNSTSYTSKTGCLSSFNMSSPM 566

Query: 541 PQMGPSKPSTFKNQYLVIQEACDETSLIAYNFIGRLLEEFAKSEGVELDWCANLYLTSVS 600
           P+M PSKPSTFKNQYL++QEACDETS+IAYNFIGRLLEEFA+SEGVELDWCANLYL+S S
Sbjct: 567 PRMEPSKPSTFKNQYLMVQEACDETSMIAYNFIGRLLEEFARSEGVELDWCANLYLSSNS 626

Query: 601 STEEDLPEDEQTHIKGNGN---FIQVCKELIPSLSK 614
           S EEDL E EQTHIK  GN    I+VC+ELIP LSK
Sbjct: 627 SIEEDLSEVEQTHIKAKGNDSVIIKVCQELIPPLSK 658

BLAST of MS022253 vs. NCBI nr
Match: XP_022928418.1 (uncharacterized protein LOC111435237 isoform X1 [Cucurbita moschata] >XP_022928419.1 uncharacterized protein LOC111435237 isoform X1 [Cucurbita moschata])

HSP 1 Score: 900.2 bits (2325), Expect = 9.8e-258
Identity = 483/656 (73.63%), Postives = 529/656 (80.64%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLGLPTS    K  DHSDFLNK+NLPE LLREDDVFYETVKTRVEEAFGALNVET   
Sbjct: 27  RWLLGLPTSVPSLKHFDHSDFLNKKNLPESLLREDDVFYETVKTRVEEAFGALNVETSRL 86

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
           GI  D++ DTCK+ KLILSCL+DLST GLYLLAII T+DSV LEKTR KLKRV++EFIP 
Sbjct: 87  GIPVDQMLDTCKVRKLILSCLNDLSTNGLYLLAIIFTEDSVGLEKTRCKLKRVIKEFIPK 146

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNF----RRRCSVTLTSSSPSYHDAASQVLYRLGD 180
           VLRR+SQDCHQLE+VK+L+QLLNDP NF    RR CS T TSS PS  DAASQVLYRLGD
Sbjct: 147 VLRRESQDCHQLEIVKRLAQLLNDPKNFRRGCRRGCSATSTSSLPSLQDAASQVLYRLGD 206

Query: 181 LPTQGLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLA 240
           LPTQGL+AM RKL GVRVMPQ+KRH+HGWGRDRLIN+LT+ S KMLSS  EGD+LQESLA
Sbjct: 207 LPTQGLIAMHRKLSGVRVMPQMKRHKHGWGRDRLINVLTKISKKMLSSLREGDKLQESLA 266

Query: 241 KAMAVADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSL 300
           KAMA+A+LSLKLV G HNSS IEFYPF PQIKTLHNEIVKAIW VRKK +F+KLKQLKSL
Sbjct: 267 KAMAMAELSLKLVSGHHNSSIIEFYPFSPQIKTLHNEIVKAIWFVRKKSDFRKLKQLKSL 326

Query: 301 LDPDAKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEE 360
           LDPD+KV NRCLRTAIK+MLIDYLFEC DMDTVPKSLLKALA+INADSR+AS+SFFS +E
Sbjct: 327 LDPDSKVPNRCLRTAIKQMLIDYLFECCDMDTVPKSLLKALAMINADSRSASHSFFSQDE 386

Query: 361 IEKEVECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCD-GLP 420
            E+EVECVFSLSAQMKQVVWDLLPN +FEH+FADAYMEELEESDDDFDD+ + +CD GLP
Sbjct: 387 TEEEVECVFSLSAQMKQVVWDLLPNREFEHEFADAYMEELEESDDDFDDDGSGSCDGGLP 446

Query: 421 LDDNGSHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHA-----------------FTG 480
            +DNGSHS     +VEGMGESMP NL +SSVGN LSPS A                 FT 
Sbjct: 447 REDNGSHSV----YVEGMGESMPGNLGYSSVGNVLSPSQASMKSPDVRPLQRSELTHFTR 506

Query: 481 ECPLDSSFKYCPSFMESKFQKDT------------------HHRDDSPSSKFTPNILLDK 540
           E  LDSSF +C SFMESK Q DT                  H  +DSPSSKFTPNILLDK
Sbjct: 507 EGSLDSSFNFCSSFMESKVQIDTYNLSSNQQVVNKDTISFSHRMNDSPSSKFTPNILLDK 566

Query: 541 STSDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQEACDETSLIAYNFIGRLLEEF 600
           ST D A T CLSS NMSCAMPQM P KP+ FKNQYLVIQEACD TS+IAYNFIGRLLEEF
Sbjct: 567 STIDLAKTPCLSSFNMSCAMPQMEPCKPNPFKNQYLVIQEACDGTSMIAYNFIGRLLEEF 626

Query: 601 AKSEGVELDWCANLYLTSVSSTEEDLPEDEQTHIKGNGN---FIQVCKELIPSLSK 614
           AKSEGV+LDWCANLYL++ SS EED  E EQTH K  G     I VC+ELIPSLS+
Sbjct: 627 AKSEGVDLDWCANLYLSTHSSIEEDPREVEQTHTKAKGGDSVIIHVCEELIPSLSE 678

BLAST of MS022253 vs. ExPASy TrEMBL
Match: A0A6J1C3N7 (uncharacterized protein LOC111007960 OS=Momordica charantia OX=3673 GN=LOC111007960 PE=4 SV=1)

HSP 1 Score: 1216.8 bits (3147), Expect = 0.0e+00
Identity = 611/615 (99.35%), Postives = 612/615 (99.51%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA
Sbjct: 27  RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 86

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
           GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN
Sbjct: 87  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 146

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNFRRRCSVTLTSSSPSYHDAASQVLYRLGDLPTQ 180
           VLRRKSQDCHQLEVVKKLSQLLNDPNNFRRRCSVTLTSSSPSYHDAASQVLYRLGDLPTQ
Sbjct: 147 VLRRKSQDCHQLEVVKKLSQLLNDPNNFRRRCSVTLTSSSPSYHDAASQVLYRLGDLPTQ 206

Query: 181 GLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLAKAMA 240
           GLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLAKAMA
Sbjct: 207 GLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLAKAMA 266

Query: 241 VADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSLLDPD 300
           VADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSLLDPD
Sbjct: 267 VADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSLLDPD 326

Query: 301 AKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEEIEKE 360
           AKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEEIEKE
Sbjct: 327 AKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEEIEKE 386

Query: 361 VECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCDGLPLDDNG 420
           VECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCDGLPLDDNG
Sbjct: 387 VECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCDGLPLDDNG 446

Query: 421 SHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHAFTGECPLDSSFKYCPSFMESKFQKD 480
           SHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHAFTGECPLDSSFKYCPSFMESKFQKD
Sbjct: 447 SHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHAFTGECPLDSSFKYCPSFMESKFQKD 506

Query: 481 THHRDDSPSSKFTPNILLDKSTSDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQE 540
           THHRDDSPSSKFTPNILLDKS+SDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQE
Sbjct: 507 THHRDDSPSSKFTPNILLDKSSSDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQE 566

Query: 541 ACDETSLIAYNFIGRLLEEFAKSEGVELDWCANLYLTSVSSTEEDLP--EDEQTHIKGNG 600
           ACDETSLIAYN IGRLLEEFAKSEGVELDWCANLYLTSVSSTEEDLP  EDEQTHIKGNG
Sbjct: 567 ACDETSLIAYNVIGRLLEEFAKSEGVELDWCANLYLTSVSSTEEDLPESEDEQTHIKGNG 626

Query: 601 NFIQVCKELIPSLSK 614
           NFIQVCKELIPSLSK
Sbjct: 627 NFIQVCKELIPSLSK 641

BLAST of MS022253 vs. ExPASy TrEMBL
Match: A0A6J1EKS9 (uncharacterized protein LOC111435237 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435237 PE=4 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.8e-258
Identity = 483/656 (73.63%), Postives = 529/656 (80.64%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLGLPTS    K  DHSDFLNK+NLPE LLREDDVFYETVKTRVEEAFGALNVET   
Sbjct: 27  RWLLGLPTSVPSLKHFDHSDFLNKKNLPESLLREDDVFYETVKTRVEEAFGALNVETSRL 86

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
           GI  D++ DTCK+ KLILSCL+DLST GLYLLAII T+DSV LEKTR KLKRV++EFIP 
Sbjct: 87  GIPVDQMLDTCKVRKLILSCLNDLSTNGLYLLAIIFTEDSVGLEKTRCKLKRVIKEFIPK 146

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNF----RRRCSVTLTSSSPSYHDAASQVLYRLGD 180
           VLRR+SQDCHQLE+VK+L+QLLNDP NF    RR CS T TSS PS  DAASQVLYRLGD
Sbjct: 147 VLRRESQDCHQLEIVKRLAQLLNDPKNFRRGCRRGCSATSTSSLPSLQDAASQVLYRLGD 206

Query: 181 LPTQGLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLA 240
           LPTQGL+AM RKL GVRVMPQ+KRH+HGWGRDRLIN+LT+ S KMLSS  EGD+LQESLA
Sbjct: 207 LPTQGLIAMHRKLSGVRVMPQMKRHKHGWGRDRLINVLTKISKKMLSSLREGDKLQESLA 266

Query: 241 KAMAVADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSL 300
           KAMA+A+LSLKLV G HNSS IEFYPF PQIKTLHNEIVKAIW VRKK +F+KLKQLKSL
Sbjct: 267 KAMAMAELSLKLVSGHHNSSIIEFYPFSPQIKTLHNEIVKAIWFVRKKSDFRKLKQLKSL 326

Query: 301 LDPDAKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEE 360
           LDPD+KV NRCLRTAIK+MLIDYLFEC DMDTVPKSLLKALA+INADSR+AS+SFFS +E
Sbjct: 327 LDPDSKVPNRCLRTAIKQMLIDYLFECCDMDTVPKSLLKALAMINADSRSASHSFFSQDE 386

Query: 361 IEKEVECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCD-GLP 420
            E+EVECVFSLSAQMKQVVWDLLPN +FEH+FADAYMEELEESDDDFDD+ + +CD GLP
Sbjct: 387 TEEEVECVFSLSAQMKQVVWDLLPNREFEHEFADAYMEELEESDDDFDDDGSGSCDGGLP 446

Query: 421 LDDNGSHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHA-----------------FTG 480
            +DNGSHS     +VEGMGESMP NL +SSVGN LSPS A                 FT 
Sbjct: 447 REDNGSHSV----YVEGMGESMPGNLGYSSVGNVLSPSQASMKSPDVRPLQRSELTHFTR 506

Query: 481 ECPLDSSFKYCPSFMESKFQKDT------------------HHRDDSPSSKFTPNILLDK 540
           E  LDSSF +C SFMESK Q DT                  H  +DSPSSKFTPNILLDK
Sbjct: 507 EGSLDSSFNFCSSFMESKVQIDTYNLSSNQQVVNKDTISFSHRMNDSPSSKFTPNILLDK 566

Query: 541 STSDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQEACDETSLIAYNFIGRLLEEF 600
           ST D A T CLSS NMSCAMPQM P KP+ FKNQYLVIQEACD TS+IAYNFIGRLLEEF
Sbjct: 567 STIDLAKTPCLSSFNMSCAMPQMEPCKPNPFKNQYLVIQEACDGTSMIAYNFIGRLLEEF 626

Query: 601 AKSEGVELDWCANLYLTSVSSTEEDLPEDEQTHIKGNGN---FIQVCKELIPSLSK 614
           AKSEGV+LDWCANLYL++ SS EED  E EQTH K  G     I VC+ELIPSLS+
Sbjct: 627 AKSEGVDLDWCANLYLSTHSSIEEDPREVEQTHTKAKGGDSVIIHVCEELIPSLSE 678

BLAST of MS022253 vs. ExPASy TrEMBL
Match: A0A6J1ERM5 (uncharacterized protein LOC111435237 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435237 PE=4 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 1.8e-257
Identity = 483/656 (73.63%), Postives = 529/656 (80.64%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLGLPTS    K  DHSDFLNK+NLPE LLREDDVFYETVKTRVEEAFGALNVET   
Sbjct: 27  RWLLGLPTSVPSLKHFDHSDFLNKKNLPESLLREDDVFYETVKTRVEEAFGALNVETSRL 86

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
           GI  D++ DTCK+ KLILSCL+DLST GLYLLAII T+DSV LEKTR KLKRV++EFIP 
Sbjct: 87  GIPVDQMLDTCKVRKLILSCLNDLSTNGLYLLAIIFTEDSVGLEKTRCKLKRVIKEFIPK 146

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNF----RRRCSVTLTSSSPSYHDAASQVLYRLGD 180
           VLRR+SQDCHQLE+VK+L+QLLNDP NF    RR CS T TSS PS  DAASQVLYRLGD
Sbjct: 147 VLRRESQDCHQLEIVKRLAQLLNDPKNFRRGCRRGCSATSTSSLPSLQDAASQVLYRLGD 206

Query: 181 LPTQGLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLA 240
           LPTQGL+AM RKL GVRVMPQ+KRH+HGWGRDRLIN+LT+ S KMLSS  EGD+LQESLA
Sbjct: 207 LPTQGLIAMHRKLSGVRVMPQMKRHKHGWGRDRLINVLTKISKKMLSSLREGDKLQESLA 266

Query: 241 KAMAVADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSL 300
           KAMA+A+LSLKLV G HNSS IEFYPF PQIKTLHNEIVKAIW VRKK +F+KLKQLKSL
Sbjct: 267 KAMAMAELSLKLVSGHHNSSIIEFYPFSPQIKTLHNEIVKAIWFVRKKSDFRKLKQLKSL 326

Query: 301 LDPDAKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEE 360
           LDPD+KV NRCLRTAIK+MLIDYLFEC DMDTVPKSLLKALA+INADSR+AS+SFFS +E
Sbjct: 327 LDPDSKVPNRCLRTAIKQMLIDYLFECCDMDTVPKSLLKALAMINADSRSASHSFFSQDE 386

Query: 361 IEKEVECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCD-GLP 420
            E+EVECVFSLSAQMKQVVWDLLPN +FEH+FADAYMEELEESDDDFDD+ + +CD GLP
Sbjct: 387 TEEEVECVFSLSAQMKQVVWDLLPNREFEHEFADAYMEELEESDDDFDDDGSGSCDGGLP 446

Query: 421 LDDNGSHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHA-----------------FTG 480
            +DNGSHS     +VEGMGESMP NL +SSVGN LSPS A                 FT 
Sbjct: 447 REDNGSHSV----YVEGMGESMPGNLGYSSVGNVLSPSQASMKSPDVRPLQRSELTHFTR 506

Query: 481 ECPLDSSFKYCPSFMESKFQKDT------------------HHRDDSPSSKFTPNILLDK 540
           E  LDSSF +C SFMESK Q DT                  H  +DSPSSKFTPNILLDK
Sbjct: 507 EGSLDSSFNFCSSFMESKVQIDTYNLSSNQQVVNKDTISFSHRMNDSPSSKFTPNILLDK 566

Query: 541 STSDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQEACDETSLIAYNFIGRLLEEF 600
           ST D A T CLSS NMSCAMPQM P KP+ FKNQYLVIQEACD TS+IAYNFIGRLLEEF
Sbjct: 567 STIDLAKTPCLSSFNMSCAMPQMEPCKPNPFKNQYLVIQEACDGTSMIAYNFIGRLLEEF 626

Query: 601 AKSEGVELDWCANLYLTSVSSTEEDLPEDEQTHIKGNGN---FIQVCKELIPSLSK 614
           AKSEGV+LDWCANLYL++ SS EED P  EQTH K  G     I VC+ELIPSLS+
Sbjct: 627 AKSEGVDLDWCANLYLSTHSSIEED-PRVEQTHTKAKGGDSVIIHVCEELIPSLSE 677

BLAST of MS022253 vs. ExPASy TrEMBL
Match: A0A6J1JJT1 (uncharacterized protein LOC111486428 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111486428 PE=4 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 7.4e-251
Identity = 474/656 (72.26%), Postives = 522/656 (79.57%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLGLPTS    K  DHSDFLNK+NLPE LLREDDVFYETV+TRVE AFG LNVET   
Sbjct: 27  RWLLGLPTSVPSLKHFDHSDFLNKKNLPESLLREDDVFYETVRTRVEGAFGTLNVETSRV 86

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
           GI  D + DTCK+ KLILSCL+DLST GLYLLA I T+DSVKLEKTR KLKRV++EFIP 
Sbjct: 87  GIPVDLMLDTCKVRKLILSCLNDLSTNGLYLLARIFTEDSVKLEKTRCKLKRVIKEFIPK 146

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNF----RRRCSVTLTSSSPSYHDAASQVLYRLGD 180
           VLRR+SQDCHQLE+V +L+QLLNDP NF    RR C+ T T S PS  DAASQVLYRLGD
Sbjct: 147 VLRRESQDCHQLEIVIRLAQLLNDPKNFRRGCRRGCAATSTPSLPSLQDAASQVLYRLGD 206

Query: 181 LPTQGLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLA 240
           LPTQGL+AM RKL GVRVMPQ+KRH+HGWGRDRLIN+LT+ S KMLSS  EGD+LQESLA
Sbjct: 207 LPTQGLIAMHRKLSGVRVMPQMKRHKHGWGRDRLINVLTKISKKMLSSLREGDKLQESLA 266

Query: 241 KAMAVADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSL 300
           KAMA+A+LSLKLV G HNSS IEFYPF PQIKTLHNEIVKAIW VRKK +F+KLKQLKSL
Sbjct: 267 KAMAMAELSLKLVSGHHNSSIIEFYPFSPQIKTLHNEIVKAIWFVRKKSDFRKLKQLKSL 326

Query: 301 LDPDAKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEE 360
           LDPD++V NRCLRTAIK+MLIDYLFEC DMDTVPKSLLKALA+INADSR+AS+SFFS +E
Sbjct: 327 LDPDSEVPNRCLRTAIKQMLIDYLFECCDMDTVPKSLLKALAMINADSRSASHSFFSQDE 386

Query: 361 IEKEVECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCD-GLP 420
            E+EVECVFSLSAQMKQVVWDLLPN +FE +FADAYMEELEESDDDFDD+ +D+CD GLP
Sbjct: 387 TEEEVECVFSLSAQMKQVVWDLLPNREFEDEFADAYMEELEESDDDFDDDGSDSCDGGLP 446

Query: 421 LDDNGSHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHA-----------------FTG 480
            +DNGSHS     ++EGMGESMP NL +SSVGN LSPS A                 FT 
Sbjct: 447 REDNGSHSV----YIEGMGESMPGNLGYSSVGNVLSPSQASMKSPDVRPLQCSELTHFTR 506

Query: 481 ECPLDSSFKYCPSFMESKFQKDT------------------HHRDDSPSSKFTPNILLDK 540
           E  LDSSF +C SFMESK Q DT                  HH  DSPSSK TPNILLD 
Sbjct: 507 EGSLDSSFNFCSSFMESKVQVDTYNLSSNQQVGNKHTISFSHHIYDSPSSKLTPNILLD- 566

Query: 541 STSDTATTACLSSLNMSCAMPQMGPSKPSTFKNQYLVIQEACDETSLIAYNFIGRLLEEF 600
            T D A T  LSS NM CAMPQM PSKPS FKNQYLVIQEACD TS+IAYNFIGRLLEEF
Sbjct: 567 -TIDLAKTPFLSSFNMPCAMPQMEPSKPSPFKNQYLVIQEACDGTSMIAYNFIGRLLEEF 626

Query: 601 AKSEGVELDWCANLYLTSVSSTEEDLPEDEQTHIKGNGN---FIQVCKELIPSLSK 614
           AKSEGV+LDWC+NLYL+S  S EEDLPE EQTH K   +    IQVC+ELIPSLS+
Sbjct: 627 AKSEGVDLDWCSNLYLSSRCSIEEDLPEVEQTHTKAKRSDSVIIQVCEELIPSLSE 676

BLAST of MS022253 vs. ExPASy TrEMBL
Match: A0A6J1GJE0 (uncharacterized protein LOC111454828 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454828 PE=4 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 9.6e-251
Identity = 469/636 (73.74%), Postives = 517/636 (81.29%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLGLPTS SG K SDHSD LNKRNLPE LLREDDVF+ TVKTRVEEAFG LN+ETRH 
Sbjct: 27  RWLLGLPTSISGPKYSDHSDILNKRNLPESLLREDDVFFGTVKTRVEEAFGVLNIETRHL 86

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
           GI+ D+I DTCK+ KLILSCLDDLSTRGLY LA IL++DSVKLEKTRWKLKRV+REFIP 
Sbjct: 87  GIRADQILDTCKVRKLILSCLDDLSTRGLYGLAKILSEDSVKLEKTRWKLKRVIREFIPK 146

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNFRRRCSVTLTSSSPSYHDAASQVLYRLGDLPTQ 180
           VL RKSQDC+QLE  K+LSQLLND  NFRR  S T TSS+ S+HDAASQVLY LGD+PTQ
Sbjct: 147 VLGRKSQDCYQLETFKRLSQLLNDTTNFRRIRSATSTSSTTSFHDAASQVLYGLGDMPTQ 206

Query: 181 GLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLAKAMA 240
            LLAMRRKLEGVR +PQIK  + GWGRDRLIN+LT+ S KMLSS GEGDELQESLAKAMA
Sbjct: 207 VLLAMRRKLEGVRFLPQIKYRKPGWGRDRLINLLTKISKKMLSSLGEGDELQESLAKAMA 266

Query: 241 VADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSLLDPD 300
           VADLSLKLVPG HNSS IEFYPF PQIKTLHNEIVKAIW V K  N +KLK+LK LLDPD
Sbjct: 267 VADLSLKLVPGRHNSSVIEFYPFSPQIKTLHNEIVKAIWFVSKNCNIEKLKRLKFLLDPD 326

Query: 301 AKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEEIEKE 360
           A+VS+RCLR  IK+ML DYLFECSDMDTVPKSLLKALA+I  DSR+A +S    +EI  E
Sbjct: 327 AEVSHRCLRAGIKRMLTDYLFECSDMDTVPKSLLKALAMIKTDSRSAPHSVLPQDEIGDE 386

Query: 361 VECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDNCDGLPLDDNG 420
           VE VFSLSAQMKQVVWDLLPNCDFEHDF DAYMEELEESDDD+DDND+D  DGLP +D+G
Sbjct: 387 VENVFSLSAQMKQVVWDLLPNCDFEHDFVDAYMEELEESDDDYDDNDDDIFDGLPQEDHG 446

Query: 421 SHSSNMIHHVEGMGESMPANLEHSSVGNGLSPSHA-----------------FTGECPLD 480
            HS      VEGMGESMPANL+++SVGN LSPS A                 FT E  LD
Sbjct: 447 PHSV----FVEGMGESMPANLDNASVGNILSPSQASLKNADVDLLECSKPTPFTREGSLD 506

Query: 481 SSFKYCPSFMESKFQKDTHH--RDDSPSSKFTPNILLD-KSTSDTATTACLSSLNMSCAM 540
           SSF Y PSFMESK Q DT++   +    SK TPN LLD ++TS T+ + CL+SLNMSC +
Sbjct: 507 SSFIYHPSFMESKVQPDTYNLSSNQQVGSKDTPNTLLDNRTTSYTSKSTCLTSLNMSCPI 566

Query: 541 PQMGPSKPSTFKNQYLVIQEACDETSLIAYNFIGRLLEEFAKSEGVELDWCANLYLTSVS 600
           PQM PSKPSTFKNQYLV+QEACDETS+IAYNFIGRLLEE+AKSEG+EL+WCANLYLT  S
Sbjct: 567 PQMEPSKPSTFKNQYLVVQEACDETSMIAYNFIGRLLEEYAKSEGIELNWCANLYLTCNS 626

Query: 601 STEEDLPEDEQTHIKGNGN---FIQVCKELIPSLSK 614
           S EEDLPE EQT IK  G     IQVC+ELIPSLSK
Sbjct: 627 SVEEDLPEVEQTRIKAKGGDSVIIQVCEELIPSLSK 658

BLAST of MS022253 vs. TAIR 10
Match: AT5G40520.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 11 growth stages; Has 3696 Blast hits to 2485 proteins in 391 species: Archae - 10; Bacteria - 571; Metazoa - 1013; Fungi - 530; Plants - 217; Viruses - 74; Other Eukaryotes - 1281 (source: NCBI BLink). )

HSP 1 Score: 364.0 bits (933), Expect = 2.4e-100
Identity = 248/660 (37.58%), Postives = 361/660 (54.70%), Query Frame = 0

Query: 1   RWLLGLPTSTSGQKCSDHSDFLNKRNLPEFLLREDDVFYETVKTRVEEAFGALNVETRHA 60
           RWLLG   S S     DH+       +PE LLREDD+FYET+K+RVEEAFG    +    
Sbjct: 33  RWLLGCDMSDS---TKDHTPG-KTEFVPESLLREDDIFYETIKSRVEEAFGFCKNQQVVC 92

Query: 61  GIQGDRIFDTCKIGKLILSCLDDLSTRGLYLLAIILTQDSVKLEKTRWKLKRVVREFIPN 120
                +    C +   ++  LD L+ +GLYL+A+ILT  S   +KTR K+K ++R+ +  
Sbjct: 93  NDVQQKELKFCDLE--LVRKLDSLTNKGLYLIAMILTGGSTSFDKTRLKMKEIIRDSVRR 152

Query: 121 VLRRKSQDCHQLEVVKKLSQLLNDPNNFRRRCSVTL--TSSSPSYHDAASQVLYRLGDLP 180
              +  +   + E++ +L Q+L+DP NFR  C + L  T +  S+ DAA +VL  L  L 
Sbjct: 153 DFGKNKEGIGKEEIINQLHQVLSDPANFREDCRMNLGRTPTLHSHRDAAMKVLNELDSLS 212

Query: 181 TQGLLAMRRKLEGVRVMPQIKRHRHGWGRDRLINILTETSMKMLSSHGEGDELQESLAKA 240
           TQ L AM+RKL+G R++PQ+K  R G  R  LIN + + S KMLS    GD+LQE LAKA
Sbjct: 213 TQTLRAMKRKLKGSRMIPQLKNSRRGQRRSDLINQVRQASEKMLSELSAGDKLQEQLAKA 272

Query: 241 MAVADLSLKLVPGCHNSSTIEFYPFPPQIKTLHNEIVKAIWSVRKKGNFQKLKQLKSLLD 300
           ++V DLSLKL PG   ++  +F+ F P+ K L NEIVKA+W +RK   F++LK+L   LD
Sbjct: 273 LSVVDLSLKLSPGYKTAAATDFFRFSPETKNLQNEIVKAVWLLRKV-RFRELKRLHLCLD 332

Query: 301 PDAKVSNRCLRTAIKKMLIDYLFECSDMDTVPKSLLKALAIINADSRNASNSFFSLEEIE 360
           P+A+VSN  LR+A++K LI+YLFECSD+DT+PKSL++AL+++N+ + N  +     E IE
Sbjct: 333 PEAEVSNDSLRSAVRKTLIEYLFECSDLDTIPKSLMEALSLVNSRTGNVEHKVCPREAIE 392

Query: 361 KEVECVFSLSAQMKQVVWDLLPNCDFEHDFADAYMEELEESDDDFDDNDNDN-------- 420
           +E EC+ ++SAQ+KQ+    +PN + + DF DAYME+LE+SDD+ DD+D+D+        
Sbjct: 393 EETECILNVSAQVKQIFCQCIPNYELDQDFGDAYMEDLEDSDDNDDDDDDDDDDDENCNL 452

Query: 421 -------------CDGLPLD-----DNGSHSSNMIHHVEGMGESM---PANLEHSSVGNG 480
                        C  + L+     D+   SS+  H   G  E +   P +  H +  + 
Sbjct: 453 FDNEKLGNKEESRCRNIKLEVKDTQDDAMESSDSDHEESG-AECLVLDPTDSTHETNQHD 512

Query: 481 LSPS--HAFTGECP------LDSSFKYCPSFMESKFQKDTHHRDDSPSSKFTPNIL--LD 540
            S S       + P         S    P+  +S    D H   D   SK    +   ++
Sbjct: 513 FSSSVNRVVVRDLPESITRVYPRSLYVTPTSNKSIVISDRH---DIAMSKTRVKVERDIE 572

Query: 541 KSTSDTATTACLSSLNMSCAMPQMGPSKP-STFKNQYLVIQEACDETSLIAYNFIGRLLE 600
               +  ++  L S+  +      G  KP    KNQYL IQE  DETSL+A+N IGRLLE
Sbjct: 573 MEVDNQFSSRSLFSVE-NIKSDDHGEQKPRRRNKNQYLAIQEISDETSLVAHNLIGRLLE 632

Query: 601 EFAKSEGVELDWCANLYLTSVSSTEEDLPEDEQTHIKGNGN-----FIQVCKELIPSLSK 614
           +FA  + + L+     YL   S  +E +   E+              + V KE I SL +
Sbjct: 633 KFADRQRLNLETDERSYLGGESRLQEKVEVSEKKQASSQAKSDEAIILSVVKEQITSLEE 680

BLAST of MS022253 vs. TAIR 10
Match: AT5G40520.1 (unknown protein; Has 3679 Blast hits to 2474 proteins in 390 species: Archae - 10; Bacteria - 571; Metazoa - 1012; Fungi - 528; Plants - 214; Viruses - 74; Other Eukaryotes - 1270 (source: NCBI BLink). )

HSP 1 Score: 307.8 bits (787), Expect = 2.0e-83
Identity = 210/567 (37.04%), Postives = 310/567 (54.67%), Query Frame = 0

Query: 94  IILTQDSVKLEKTRWKLKRVVREFIPNVLRRKSQDCHQLEVVKKLSQLLNDPNNFRRRCS 153
           +ILT  S   +KTR K+K ++R+ +     +  +   + E++ +L Q+L+DP NFR  C 
Sbjct: 1   MILTGGSTSFDKTRLKMKEIIRDSVRRDFGKNKEGIGKEEIINQLHQVLSDPANFREDCR 60

Query: 154 VTL--TSSSPSYHDAASQVLYRLGDLPTQGLLAMRRKLEGVRVMPQIKRHRHGWGRDRLI 213
           + L  T +  S+ DAA +VL  L  L TQ L AM+RKL+G R++PQ+K  R G  R  LI
Sbjct: 61  MNLGRTPTLHSHRDAAMKVLNELDSLSTQTLRAMKRKLKGSRMIPQLKNSRRGQRRSDLI 120

Query: 214 NILTETSMKMLSSHGEGDELQESLAKAMAVADLSLKLVPGCHNSSTIEFYPFPPQIKTLH 273
           N + + S KMLS    GD+LQE LAKA++V DLSLKL PG   ++  +F+ F P+ K L 
Sbjct: 121 NQVRQASEKMLSELSAGDKLQEQLAKALSVVDLSLKLSPGYKTAAATDFFRFSPETKNLQ 180

Query: 274 NEIVKAIWSVRKKGNFQKLKQLKSLLDPDAKVSNRCLRTAIKKMLIDYLFECSDMDTVPK 333
           NEIVKA+W +RK   F++LK+L   LDP+A+VSN  LR+A++K LI+YLFECSD+DT+PK
Sbjct: 181 NEIVKAVWLLRKV-RFRELKRLHLCLDPEAEVSNDSLRSAVRKTLIEYLFECSDLDTIPK 240

Query: 334 SLLKALAIINADSRNASNSFFSLEEIEKEVECVFSLSAQMKQVVWDLLPNCDFEHDFADA 393
           SL++AL+++N+ + N  +     E IE+E EC+ ++SAQ+KQ+    +PN + + DF DA
Sbjct: 241 SLMEALSLVNSRTGNVEHKVCPREAIEEETECILNVSAQVKQIFCQCIPNYELDQDFGDA 300

Query: 394 YMEELEESDDDFDDNDNDN---------------------CDGLPLD-----DNGSHSSN 453
           YME+LE+SDD+ DD+D+D+                     C  + L+     D+   SS+
Sbjct: 301 YMEDLEDSDDNDDDDDDDDDDDENCNLFDNEKLGNKEESRCRNIKLEVKDTQDDAMESSD 360

Query: 454 MIHHVEGMGESM---PANLEHSSVGNGLSPS--HAFTGECP------LDSSFKYCPSFME 513
             H   G  E +   P +  H +  +  S S       + P         S    P+  +
Sbjct: 361 SDHEESG-AECLVLDPTDSTHETNQHDFSSSVNRVVVRDLPESITRVYPRSLYVTPTSNK 420

Query: 514 SKFQKDTHHRDDSPSSKFTPNIL--LDKSTSDTATTACLSSLNMSCAMPQMGPSKP-STF 573
           S    D H   D   SK    +   ++    +  ++  L S+  +      G  KP    
Sbjct: 421 SIVISDRH---DIAMSKTRVKVERDIEMEVDNQFSSRSLFSVE-NIKSDDHGEQKPRRRN 480

Query: 574 KNQYLVIQEACDETSLIAYNFIGRLLEEFAKSEGVELDWCANLYLTSVSSTEEDLPEDEQ 614
           KNQYL IQE  DETSL+A+N IGRLLE+FA  + + L+     YL   S  +E +   E+
Sbjct: 481 KNQYLAIQEISDETSLVAHNLIGRLLEKFADRQRLNLETDERSYLGGESRLQEKVEVSEK 540