MS022232 (gene) Bitter gourd (TR) v1

Overview
NameMS022232
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionF-box domain-containing protein
Locationscaffold47: 1884798 .. 1885187 (-)
RNA-Seq ExpressionMS022232
SyntenyMS022232
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCGTCCATGGGAAGATCTGCCAAAGGATGTTATGGAGAAAATTGGTTTGAATCTTAATTCAACTGATTACATTCATGCCTCTGTTGTTTGCAAATCATGGCTATCACTTTTACAATCTGATATTTCAAGACCTCAAATTCCTTGGATTTTGCTTCCTCGTCAATCAGATGACCATAGCCTAAAATTCTTCAACTTTTTGGAAGCCAAATATCACATCTTAAGGTTGCCTAAGGGTCTTGAAGGGGGATATATTTTTGGGAGTTCCAAAGGATGGCTTGTTGTTGTGTTTAGGAGAAGAAGAAGATCAGGATGGTACCATGTCTATCTTTTGAATCCAATCTCAGGTGTCAAGTGTAGGCTTCCGCCGCTTCCAAATTATATATCTCTT

mRNA sequence

TCTCGTCCATGGGAAGATCTGCCAAAGGATGTTATGGAGAAAATTGGTTTGAATCTTAATTCAACTGATTACATTCATGCCTCTGTTGTTTGCAAATCATGGCTATCACTTTTACAATCTGATATTTCAAGACCTCAAATTCCTTGGATTTTGCTTCCTCGTCAATCAGATGACCATAGCCTAAAATTCTTCAACTTTTTGGAAGCCAAATATCACATCTTAAGGTTGCCTAAGGGTCTTGAAGGGGGATATATTTTTGGGAGTTCCAAAGGATGGCTTGTTGTTGTGTTTAGGAGAAGAAGAAGATCAGGATGGTACCATGTCTATCTTTTGAATCCAATCTCAGGTGTCAAGTGTAGGCTTCCGCCGCTTCCAAATTATATATCTCTT

Coding sequence (CDS)

TCTCGTCCATGGGAAGATCTGCCAAAGGATGTTATGGAGAAAATTGGTTTGAATCTTAATTCAACTGATTACATTCATGCCTCTGTTGTTTGCAAATCATGGCTATCACTTTTACAATCTGATATTTCAAGACCTCAAATTCCTTGGATTTTGCTTCCTCGTCAATCAGATGACCATAGCCTAAAATTCTTCAACTTTTTGGAAGCCAAATATCACATCTTAAGGTTGCCTAAGGGTCTTGAAGGGGGATATATTTTTGGGAGTTCCAAAGGATGGCTTGTTGTTGTGTTTAGGAGAAGAAGAAGATCAGGATGGTACCATGTCTATCTTTTGAATCCAATCTCAGGTGTCAAGTGTAGGCTTCCGCCGCTTCCAAATTATATATCTCTT

Protein sequence

SRPWEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISRPQIPWILLPRQSDDHSLKFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRLPPLPNYISL
Homology
BLAST of MS022232 vs. NCBI nr
Match: XP_022155350.1 (uncharacterized protein LOC111022492 [Momordica charantia])

HSP 1 Score: 125.2 bits (313), Expect = 4.2e-25
Identity = 63/129 (48.84%), Postives = 81/129 (62.79%), Query Frame = 0

Query: 3   PWEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLS-LLQSDISRPQIPWILLPRQSDDHSL 62
           PW++LP D+MEKI L LNSTDYI  S+VCKSW S LLQSDI RPQ PW+LLP  S  H++
Sbjct: 27  PWKNLPGDLMEKIALKLNSTDYIRNSMVCKSWQSLLLQSDILRPQFPWLLLPHHSSKHTI 86

Query: 63  KFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 122
           K ++  E K+H   +     G  + G SKGW++       ++    + L NPISG KC L
Sbjct: 87  KLYSVSENKFHTFHVD---GGNNVVGCSKGWVIF------KTVGSKISLFNPISGAKCIL 146

Query: 123 PPLPNYISL 131
             LP  +SL
Sbjct: 147 SSLPTALSL 146

BLAST of MS022232 vs. NCBI nr
Match: KAE8055702.1 (hypothetical protein FH972_012527 [Carpinus fangiana])

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-20
Identity = 54/124 (43.55%), Postives = 75/124 (60.48%), Query Frame = 0

Query: 4   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISR--PQIPWILLPRQSDDHSL 63
           W++LP  +ME I   LN  D +H+S VCKSW SL+     R  PQ+PW++LP +   +SL
Sbjct: 40  WDELPDGIMENIFGRLNMIDLVHSSGVCKSWRSLVMQKQVRAAPQLPWLVLPHRQSCNSL 99

Query: 64  KFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWY-HVYLLNPISGVKCR 123
            F++  EA  + ++LPK  +GG+ FG SK WL++     R + W   VYL NP SG K  
Sbjct: 100 SFYSMAEATVYNIKLPKPAQGGWCFGCSKSWLMMA----RGTEWEPEVYLFNPFSGFKLN 159

Query: 124 LPPL 125
           LP L
Sbjct: 160 LPSL 159

BLAST of MS022232 vs. NCBI nr
Match: KAE8008804.1 (hypothetical protein FH972_005279 [Carpinus fangiana])

HSP 1 Score: 109.8 bits (273), Expect = 1.8e-20
Identity = 57/123 (46.34%), Postives = 76/123 (61.79%), Query Frame = 0

Query: 4   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPRQSDDHSL 63
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLP   + +SL
Sbjct: 60  WDEVPYDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNCNSL 119

Query: 64  KFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 123
            FF+  E   H ++LPK  +GG+ FGSSKGWL +    R       V+LLNPISGV+  L
Sbjct: 120 SFFSMSEGIVHSIKLPKAAQGGWCFGSSKGWLTIA---RGTQDDPQVFLLNPISGVQRHL 179

Query: 124 PPL 125
           P L
Sbjct: 180 PSL 179

BLAST of MS022232 vs. NCBI nr
Match: KAE8008846.1 (hypothetical protein FH972_005319 [Carpinus fangiana])

HSP 1 Score: 107.1 bits (266), Expect = 1.2e-19
Identity = 55/123 (44.72%), Postives = 76/123 (61.79%), Query Frame = 0

Query: 4   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPRQSDDHSL 63
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLP   + +SL
Sbjct: 52  WDEVPDDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNCNSL 111

Query: 64  KFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 123
            FF+  E   H ++LP+  +GG+ FGSSKGWL +    R       V+LL+PISGV+  L
Sbjct: 112 SFFSMSEGIVHTIKLPEAAQGGWCFGSSKGWLTIA---RGTQDDPQVFLLSPISGVQRHL 171

Query: 124 PPL 125
           P L
Sbjct: 172 PSL 171

BLAST of MS022232 vs. NCBI nr
Match: XP_004297987.1 (PREDICTED: uncharacterized protein LOC101310695 [Fragaria vesca subsp. vesca])

HSP 1 Score: 106.3 bits (264), Expect = 2.0e-19
Identity = 53/123 (43.09%), Postives = 77/123 (62.60%), Query Frame = 0

Query: 4   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDI--SRPQIPWILLPRQSDDHSL 63
           WE LP D+M  I  +L+  D I  S+VCK W S+       S PQ+PW+LLP+ S +H L
Sbjct: 31  WETLPDDIMGMIVSHLSVRDRIRLSIVCKPWSSVSTRPYIPSAPQLPWLLLPQSSPNH-L 90

Query: 64  KFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 123
            +F   E K   L LP+ ++GG+  GSSKGW++++  +   S    ++L+NPISGV+ +L
Sbjct: 91  SYFELSEGKIDRLELPEPVQGGWFHGSSKGWMIMIMEKDLDS---TMFLVNPISGVQHQL 149

Query: 124 PPL 125
           PPL
Sbjct: 151 PPL 149

BLAST of MS022232 vs. ExPASy Swiss-Prot
Match: Q9FVS1 (F-box/kelch-repeat protein At1g57790 OS=Arabidopsis thaliana OX=3702 GN=At1g57790 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 6.3e-08
Identity = 34/119 (28.57%), Postives = 56/119 (47.06%), Query Frame = 0

Query: 4   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISRPQIPWILLPRQSDDHSLKF 63
           W+DLP +++  +   L   D + ASVVCKSW     S     + PW++   ++  ++  F
Sbjct: 13  WKDLPLELLSSVMTFLEIKDNVRASVVCKSWFEAAVSVRVIDKSPWLMYFPET-KNTYDF 72

Query: 64  FNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRLP 123
           ++    K + + LPK L G  +  S  GWL++      +    H  L NP +     LP
Sbjct: 73  YDPSNCKKYTMELPKSLVGFIVRYSKDGWLLM-----SQEDSSHFVLFNPFTMDVVALP 125

BLAST of MS022232 vs. ExPASy Swiss-Prot
Match: Q9LSA5 (F-box/kelch-repeat protein At3g18720 OS=Arabidopsis thaliana OX=3702 GN=At3g18720 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 5.4e-07
Identity = 38/126 (30.16%), Postives = 61/126 (48.41%), Query Frame = 0

Query: 6   DLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISRPQIPWILLPRQS----DDHSL 65
           DL ++++ ++GL  N    IHAS+VCK+WL    S       PW+  P+       +   
Sbjct: 55  DLLQEILSRLGLKAN----IHASLVCKTWLKEAVSVRKFQSRPWLFYPQSQRGGPKEGDY 114

Query: 66  KFFNFLEAKYHILRLPKGLEG--GYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKC 125
             FN   ++ H L+ P+ L G    +  +  GWL+VV     +     V+ LNP +G + 
Sbjct: 115 VLFNPSRSQTHHLKFPE-LTGYRNKLACAKDGWLLVV-----KDNPDVVFFLNPFTGERI 170

BLAST of MS022232 vs. ExPASy TrEMBL
Match: A0A6J1DM79 (uncharacterized protein LOC111022492 OS=Momordica charantia OX=3673 GN=LOC111022492 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.0e-25
Identity = 63/129 (48.84%), Postives = 81/129 (62.79%), Query Frame = 0

Query: 3   PWEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLS-LLQSDISRPQIPWILLPRQSDDHSL 62
           PW++LP D+MEKI L LNSTDYI  S+VCKSW S LLQSDI RPQ PW+LLP  S  H++
Sbjct: 27  PWKNLPGDLMEKIALKLNSTDYIRNSMVCKSWQSLLLQSDILRPQFPWLLLPHHSSKHTI 86

Query: 63  KFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 122
           K ++  E K+H   +     G  + G SKGW++       ++    + L NPISG KC L
Sbjct: 87  KLYSVSENKFHTFHVD---GGNNVVGCSKGWVIF------KTVGSKISLFNPISGAKCIL 146

Query: 123 PPLPNYISL 131
             LP  +SL
Sbjct: 147 SSLPTALSL 146

BLAST of MS022232 vs. ExPASy TrEMBL
Match: A0A5N6R432 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_012527 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 6.8e-21
Identity = 54/124 (43.55%), Postives = 75/124 (60.48%), Query Frame = 0

Query: 4   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISR--PQIPWILLPRQSDDHSL 63
           W++LP  +ME I   LN  D +H+S VCKSW SL+     R  PQ+PW++LP +   +SL
Sbjct: 40  WDELPDGIMENIFGRLNMIDLVHSSGVCKSWRSLVMQKQVRAAPQLPWLVLPHRQSCNSL 99

Query: 64  KFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWY-HVYLLNPISGVKCR 123
            F++  EA  + ++LPK  +GG+ FG SK WL++     R + W   VYL NP SG K  
Sbjct: 100 SFYSMAEATVYNIKLPKPAQGGWCFGCSKSWLMMA----RGTEWEPEVYLFNPFSGFKLN 159

Query: 124 LPPL 125
           LP L
Sbjct: 160 LPSL 159

BLAST of MS022232 vs. ExPASy TrEMBL
Match: A0A5N6QP56 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005279 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 8.9e-21
Identity = 57/123 (46.34%), Postives = 76/123 (61.79%), Query Frame = 0

Query: 4   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPRQSDDHSL 63
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLP   + +SL
Sbjct: 60  WDEVPYDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNCNSL 119

Query: 64  KFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 123
            FF+  E   H ++LPK  +GG+ FGSSKGWL +    R       V+LLNPISGV+  L
Sbjct: 120 SFFSMSEGIVHSIKLPKAAQGGWCFGSSKGWLTIA---RGTQDDPQVFLLNPISGVQRHL 179

Query: 124 PPL 125
           P L
Sbjct: 180 PSL 179

BLAST of MS022232 vs. ExPASy TrEMBL
Match: A0A5N6QPU6 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005319 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 5.7e-20
Identity = 55/123 (44.72%), Postives = 76/123 (61.79%), Query Frame = 0

Query: 4   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPRQSDDHSL 63
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLP   + +SL
Sbjct: 52  WDEVPDDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNCNSL 111

Query: 64  KFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 123
            FF+  E   H ++LP+  +GG+ FGSSKGWL +    R       V+LL+PISGV+  L
Sbjct: 112 SFFSMSEGIVHTIKLPEAAQGGWCFGSSKGWLTIA---RGTQDDPQVFLLSPISGVQRHL 171

Query: 124 PPL 125
           P L
Sbjct: 172 PSL 171

BLAST of MS022232 vs. ExPASy TrEMBL
Match: A0A5N6QRN4 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005278 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.8e-19
Identity = 56/123 (45.53%), Postives = 73/123 (59.35%), Query Frame = 0

Query: 4   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPRQSDDHSL 63
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLP     +SL
Sbjct: 63  WDEVPDDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAPICNSL 122

Query: 64  KFFNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 123
            FF+  E   H ++LPK   GG+ FGSSKGWL +    R       V+LLNPISG +  L
Sbjct: 123 SFFSMSEGIVHNIKLPKAAHGGWCFGSSKGWLTIA---RGTHDDPQVFLLNPISGFQRHL 182

Query: 124 PPL 125
           P L
Sbjct: 183 PSL 182

BLAST of MS022232 vs. TAIR 10
Match: AT1G57790.1 (F-box family protein )

HSP 1 Score: 58.5 bits (140), Expect = 4.5e-09
Identity = 34/119 (28.57%), Postives = 56/119 (47.06%), Query Frame = 0

Query: 4   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISRPQIPWILLPRQSDDHSLKF 63
           W+DLP +++  +   L   D + ASVVCKSW     S     + PW++   ++  ++  F
Sbjct: 13  WKDLPLELLSSVMTFLEIKDNVRASVVCKSWFEAAVSVRVIDKSPWLMYFPET-KNTYDF 72

Query: 64  FNFLEAKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRLP 123
           ++    K + + LPK L G  +  S  GWL++      +    H  L NP +     LP
Sbjct: 73  YDPSNCKKYTMELPKSLVGFIVRYSKDGWLLM-----SQEDSSHFVLFNPFTMDVVALP 125

BLAST of MS022232 vs. TAIR 10
Match: AT3G18720.1 (F-box family protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.8e-08
Identity = 38/126 (30.16%), Postives = 61/126 (48.41%), Query Frame = 0

Query: 6   DLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISRPQIPWILLPRQS----DDHSL 65
           DL ++++ ++GL  N    IHAS+VCK+WL    S       PW+  P+       +   
Sbjct: 55  DLLQEILSRLGLKAN----IHASLVCKTWLKEAVSVRKFQSRPWLFYPQSQRGGPKEGDY 114

Query: 66  KFFNFLEAKYHILRLPKGLEG--GYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKC 125
             FN   ++ H L+ P+ L G    +  +  GWL+VV     +     V+ LNP +G + 
Sbjct: 115 VLFNPSRSQTHHLKFPE-LTGYRNKLACAKDGWLLVV-----KDNPDVVFFLNPFTGERI 170

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155350.14.2e-2548.84uncharacterized protein LOC111022492 [Momordica charantia][more]
KAE8055702.11.4e-2043.55hypothetical protein FH972_012527 [Carpinus fangiana][more]
KAE8008804.11.8e-2046.34hypothetical protein FH972_005279 [Carpinus fangiana][more]
KAE8008846.11.2e-1944.72hypothetical protein FH972_005319 [Carpinus fangiana][more]
XP_004297987.12.0e-1943.09PREDICTED: uncharacterized protein LOC101310695 [Fragaria vesca subsp. vesca][more]
Match NameE-valueIdentityDescription
Q9FVS16.3e-0828.57F-box/kelch-repeat protein At1g57790 OS=Arabidopsis thaliana OX=3702 GN=At1g5779... [more]
Q9LSA55.4e-0730.16F-box/kelch-repeat protein At3g18720 OS=Arabidopsis thaliana OX=3702 GN=At3g1872... [more]
Match NameE-valueIdentityDescription
A0A6J1DM792.0e-2548.84uncharacterized protein LOC111022492 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A5N6R4326.8e-2143.55F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_012527 P... [more]
A0A5N6QP568.9e-2146.34F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005279 P... [more]
A0A5N6QPU65.7e-2044.72F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005319 P... [more]
A0A5N6QRN42.8e-1945.53F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005278 P... [more]
Match NameE-valueIdentityDescription
AT1G57790.14.5e-0928.57F-box family protein [more]
AT3G18720.13.8e-0830.16F-box family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 7..47
e-value: 6.2E-4
score: 29.1
IPR001810F-box domainPFAMPF00646F-boxcoord: 3..43
e-value: 1.7E-6
score: 27.8
IPR001810F-box domainPROSITEPS50181FBOXcoord: 1..47
score: 9.153042
NoneNo IPR availableGENE3D1.20.1280.50coord: 3..41
e-value: 7.3E-7
score: 30.9
NoneNo IPR availablePANTHERPTHR44259OS07G0183000 PROTEIN-RELATEDcoord: 3..124
NoneNo IPR availablePANTHERPTHR44259:SF65BNAC03G14360D PROTEINcoord: 3..124
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 3..42

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS022232.1MS022232.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding