MS022231 (gene) Bitter gourd (TR) v1

Overview
NameMS022231
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionF-box domain-containing protein
Locationscaffold47: 1875169 .. 1875642 (-)
RNA-Seq ExpressionMS022231
SyntenyMS022231
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCAAAAAAATGAGGATGAAAATATACCATATGATCCATGGAATAGTGTGCCAAAAGATATCATGGAGGGAATTGCTTTGCATTTTAGTTCAACTGATTATATTCATAGCTCTATGGTTTGCAAATCATGGCAAGCACTTCTTCTGCAATCTGATATTGTTAGGCCAAAAGTGCTTTGGCTTTTGCTTCCACATCGCTCAACTAGAGACACCATGCAATTCTACAATGTTTTGGAGGAAAAATTTCACACCATTGTGTTGCCAAAGGTCATTGATGGGAGTAGGGTTTTTGTTGGGGGGTGTTCCAAAGGTTGGGTTATTTTCATGGATAAAGTTACGTTCGAGATCTCTCTTTTTAATCCCATCACTCGGGTCGAGTACTTCCTTCCATCGTCACCAATCATGTCGCCTTTACAGCGCGTTGAATTATCTTCATCGAATATTTCAGAGTGTACTGTGGCTGCATTCTTTGAC

mRNA sequence

TTTCAAAAAAATGAGGATGAAAATATACCATATGATCCATGGAATAGTGTGCCAAAAGATATCATGGAGGGAATTGCTTTGCATTTTAGTTCAACTGATTATATTCATAGCTCTATGGTTTGCAAATCATGGCAAGCACTTCTTCTGCAATCTGATATTGTTAGGCCAAAAGTGCTTTGGCTTTTGCTTCCACATCGCTCAACTAGAGACACCATGCAATTCTACAATGTTTTGGAGGAAAAATTTCACACCATTGTGTTGCCAAAGGTCATTGATGGGAGTAGGGTTTTTGTTGGGGGGTGTTCCAAAGGTTGGGTTATTTTCATGGATAAAGTTACGTTCGAGATCTCTCTTTTTAATCCCATCACTCGGGTCGAGTACTTCCTTCCATCGTCACCAATCATGTCGCCTTTACAGCGCGTTGAATTATCTTCATCGAATATTTCAGAGTGTACTGTGGCTGCATTCTTTGAC

Coding sequence (CDS)

TTTCAAAAAAATGAGGATGAAAATATACCATATGATCCATGGAATAGTGTGCCAAAAGATATCATGGAGGGAATTGCTTTGCATTTTAGTTCAACTGATTATATTCATAGCTCTATGGTTTGCAAATCATGGCAAGCACTTCTTCTGCAATCTGATATTGTTAGGCCAAAAGTGCTTTGGCTTTTGCTTCCACATCGCTCAACTAGAGACACCATGCAATTCTACAATGTTTTGGAGGAAAAATTTCACACCATTGTGTTGCCAAAGGTCATTGATGGGAGTAGGGTTTTTGTTGGGGGGTGTTCCAAAGGTTGGGTTATTTTCATGGATAAAGTTACGTTCGAGATCTCTCTTTTTAATCCCATCACTCGGGTCGAGTACTTCCTTCCATCGTCACCAATCATGTCGCCTTTACAGCGCGTTGAATTATCTTCATCGAATATTTCAGAGTGTACTGTGGCTGCATTCTTTGAC

Protein sequence

FQKNEDENIPYDPWNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDIVRPKVLWLLLPHRSTRDTMQFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFMDKVTFEISLFNPITRVEYFLPSSPIMSPLQRVELSSSNISECTVAAFFD
Homology
BLAST of MS022231 vs. NCBI nr
Match: XP_022155350.1 (uncharacterized protein LOC111022492 [Momordica charantia])

HSP 1 Score: 160.6 bits (405), Expect = 1.1e-35
Identity = 86/145 (59.31%), Postives = 103/145 (71.03%), Query Frame = 0

Query: 11  YDPWNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDIVRPKVLWLLLPHRSTRD 70
           Y PW ++P D+ME IAL  +STDYI +SMVCKSWQ+LLLQSDI+RP+  WLLLPH S++ 
Sbjct: 25  YGPWKNLPGDLMEKIALKLNSTDYIRNSMVCKSWQSLLLQSDILRPQFPWLLLPHHSSKH 84

Query: 71  TMQFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFMDKVTFEISLFNPITRVEYFLP 130
           T++ Y+V E KFHT      +DG    V GCSKGWVIF   V  +ISLFNPI+  +  L 
Sbjct: 85  TIKLYSVSENKFHTF----HVDGGNNVV-GCSKGWVIF-KTVGSKISLFNPISGAKCILS 144

Query: 131 SSPIMSPLQRVELSSSNISECTVAA 156
           S P    LQRVELSSS ISECTV A
Sbjct: 145 SLPTALSLQRVELSSSIISECTVVA 163

BLAST of MS022231 vs. NCBI nr
Match: VVA31268.1 (PREDICTED: F-box/kelch-repeat [Prunus dulcis])

HSP 1 Score: 95.5 bits (236), Expect = 4.3e-16
Identity = 50/121 (41.32%), Postives = 76/121 (62.81%), Query Frame = 0

Query: 14  WNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDIVR-PKVLWLLLPHRSTRDTM 73
           W +VP DIME I  H +  + I   ++CKSW +L ++ D    P++ WLLLP  S +  +
Sbjct: 3   WQTVPDDIMEIILKHLNLRERIRLRIICKSWNSLAMRRDFSNAPQLPWLLLPQASNK-YL 62

Query: 74  QFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFMDK--VTFEISLFNPITRVEYFLP 132
            F +V + KFHT++LPK + G RV   GCSKGW++ + +  +  ++ L NPI+R +Y LP
Sbjct: 63  SFSSVSDIKFHTLMLPKPVRGGRVC--GCSKGWLVMIKEKVLNSKLYLVNPISRDQYQLP 120

BLAST of MS022231 vs. NCBI nr
Match: KAE8008804.1 (hypothetical protein FH972_005279 [Carpinus fangiana])

HSP 1 Score: 94.7 bits (234), Expect = 7.4e-16
Identity = 60/167 (35.93%), Postives = 88/167 (52.69%), Query Frame = 0

Query: 11  YDPWNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDI-VRPKVLWLLLPHRSTR 70
           Y  W+ VP D+M  I    +  D I    VCKSW++++ Q DI   P V WLLLPH    
Sbjct: 57  YGRWDEVPYDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNC 116

Query: 71  DTMQFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFM--DKVTFEISLFNPITRVEY 130
           +++ F+++ E   H+I LPK   G   F  G SKGW+      +   ++ L NPI+ V+ 
Sbjct: 117 NSLSFFSMSEGIVHSIKLPKAAQGGWCF--GSSKGWLTIARGTQDDPQVFLLNPISGVQR 176

Query: 131 FLPS-----------------SPIMSPLQRVELSSSNISECTVAAFF 158
            LPS                 + I S ++++ELSS++IS+C VAA F
Sbjct: 177 HLPSLTTIPSFKDFVDVAWDPNRITSFIRKIELSSTDISKCIVAAIF 221

BLAST of MS022231 vs. NCBI nr
Match: XP_021818908.1 (uncharacterized protein LOC110760869 [Prunus avium])

HSP 1 Score: 92.4 bits (228), Expect = 3.7e-15
Identity = 48/121 (39.67%), Postives = 76/121 (62.81%), Query Frame = 0

Query: 14  WNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDIVR-PKVLWLLLPHRSTRDTM 73
           W ++P DIME I  H +  + I  +++CKSW +L ++ D    P++ WLLLP  S +  +
Sbjct: 42  WQTLPDDIMEIILKHLNLRERIRLTIICKSWNSLAMRRDFSNAPQLPWLLLPQASNK-YL 101

Query: 74  QFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFMDK--VTFEISLFNPITRVEYFLP 132
            F +V + KFHT+ LPK + G RV   GCSKGW++ + +  +  ++ L NPI+R ++ LP
Sbjct: 102 SFSSVSDGKFHTLRLPKPVRGGRVC--GCSKGWLVMIKEKVLNSKLYLINPISRDQHQLP 159

BLAST of MS022231 vs. NCBI nr
Match: KAE8008846.1 (hypothetical protein FH972_005319 [Carpinus fangiana])

HSP 1 Score: 92.0 bits (227), Expect = 4.8e-15
Identity = 58/167 (34.73%), Postives = 88/167 (52.69%), Query Frame = 0

Query: 11  YDPWNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDI-VRPKVLWLLLPHRSTR 70
           Y  W+ VP D+M  I    +  D I    VCKSW++++ Q DI   P V WLLLPH    
Sbjct: 49  YGRWDEVPDDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNC 108

Query: 71  DTMQFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFM--DKVTFEISLFNPITRVEY 130
           +++ F+++ E   HTI LP+   G   F  G SKGW+      +   ++ L +PI+ V+ 
Sbjct: 109 NSLSFFSMSEGIVHTIKLPEAAQGGWCF--GSSKGWLTIARGTQDDPQVFLLSPISGVQR 168

Query: 131 FLPS-----------------SPIMSPLQRVELSSSNISECTVAAFF 158
            LPS                 + I S ++++ELSS+++S+C VAA F
Sbjct: 169 HLPSLTAIPYFKDFVDLAWDPNRITSFIRKIELSSTDLSKCVVAATF 213

BLAST of MS022231 vs. ExPASy TrEMBL
Match: A0A6J1DM79 (uncharacterized protein LOC111022492 OS=Momordica charantia OX=3673 GN=LOC111022492 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 5.3e-36
Identity = 86/145 (59.31%), Postives = 103/145 (71.03%), Query Frame = 0

Query: 11  YDPWNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDIVRPKVLWLLLPHRSTRD 70
           Y PW ++P D+ME IAL  +STDYI +SMVCKSWQ+LLLQSDI+RP+  WLLLPH S++ 
Sbjct: 25  YGPWKNLPGDLMEKIALKLNSTDYIRNSMVCKSWQSLLLQSDILRPQFPWLLLPHHSSKH 84

Query: 71  TMQFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFMDKVTFEISLFNPITRVEYFLP 130
           T++ Y+V E KFHT      +DG    V GCSKGWVIF   V  +ISLFNPI+  +  L 
Sbjct: 85  TIKLYSVSENKFHTF----HVDGGNNVV-GCSKGWVIF-KTVGSKISLFNPISGAKCILS 144

Query: 131 SSPIMSPLQRVELSSSNISECTVAA 156
           S P    LQRVELSSS ISECTV A
Sbjct: 145 SLPTALSLQRVELSSSIISECTVVA 163

BLAST of MS022231 vs. ExPASy TrEMBL
Match: A0A5E4FUV8 (PREDICTED: F-box/kelch-repeat OS=Prunus dulcis OX=3755 GN=ALMOND_2B015541 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.1e-16
Identity = 50/121 (41.32%), Postives = 76/121 (62.81%), Query Frame = 0

Query: 14  WNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDIVR-PKVLWLLLPHRSTRDTM 73
           W +VP DIME I  H +  + I   ++CKSW +L ++ D    P++ WLLLP  S +  +
Sbjct: 3   WQTVPDDIMEIILKHLNLRERIRLRIICKSWNSLAMRRDFSNAPQLPWLLLPQASNK-YL 62

Query: 74  QFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFMDK--VTFEISLFNPITRVEYFLP 132
            F +V + KFHT++LPK + G RV   GCSKGW++ + +  +  ++ L NPI+R +Y LP
Sbjct: 63  SFSSVSDIKFHTLMLPKPVRGGRVC--GCSKGWLVMIKEKVLNSKLYLVNPISRDQYQLP 120

BLAST of MS022231 vs. ExPASy TrEMBL
Match: A0A5N6QP56 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005279 PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 3.6e-16
Identity = 60/167 (35.93%), Postives = 88/167 (52.69%), Query Frame = 0

Query: 11  YDPWNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDI-VRPKVLWLLLPHRSTR 70
           Y  W+ VP D+M  I    +  D I    VCKSW++++ Q DI   P V WLLLPH    
Sbjct: 57  YGRWDEVPYDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNC 116

Query: 71  DTMQFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFM--DKVTFEISLFNPITRVEY 130
           +++ F+++ E   H+I LPK   G   F  G SKGW+      +   ++ L NPI+ V+ 
Sbjct: 117 NSLSFFSMSEGIVHSIKLPKAAQGGWCF--GSSKGWLTIARGTQDDPQVFLLNPISGVQR 176

Query: 131 FLPS-----------------SPIMSPLQRVELSSSNISECTVAAFF 158
            LPS                 + I S ++++ELSS++IS+C VAA F
Sbjct: 177 HLPSLTTIPSFKDFVDVAWDPNRITSFIRKIELSSTDISKCIVAAIF 221

BLAST of MS022231 vs. ExPASy TrEMBL
Match: A0A6P5SZ44 (uncharacterized protein LOC110760869 OS=Prunus avium OX=42229 GN=LOC110760869 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.8e-15
Identity = 48/121 (39.67%), Postives = 76/121 (62.81%), Query Frame = 0

Query: 14  WNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDIVR-PKVLWLLLPHRSTRDTM 73
           W ++P DIME I  H +  + I  +++CKSW +L ++ D    P++ WLLLP  S +  +
Sbjct: 42  WQTLPDDIMEIILKHLNLRERIRLTIICKSWNSLAMRRDFSNAPQLPWLLLPQASNK-YL 101

Query: 74  QFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFMDK--VTFEISLFNPITRVEYFLP 132
            F +V + KFHT+ LPK + G RV   GCSKGW++ + +  +  ++ L NPI+R ++ LP
Sbjct: 102 SFSSVSDGKFHTLRLPKPVRGGRVC--GCSKGWLVMIKEKVLNSKLYLINPISRDQHQLP 159

BLAST of MS022231 vs. ExPASy TrEMBL
Match: A0A5N6R432 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_012527 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.3e-15
Identity = 47/134 (35.07%), Postives = 75/134 (55.97%), Query Frame = 0

Query: 1   FQKNEDENIPYDPWNSVPKDIMEGIALHFSSTDYIHSSMVCKSWQALLLQSDI-VRPKVL 60
           F    ++   +  W+ +P  IME I    +  D +HSS VCKSW++L++Q  +   P++ 
Sbjct: 27  FSNTTEKQHYFGRWDELPDGIMENIFGRLNMIDLVHSSGVCKSWRSLVMQKQVRAAPQLP 86

Query: 61  WLLLPHRSTRDTMQFYNVLEEKFHTIVLPKVIDGSRVFVGGCSKGWVIFMDKVTF--EIS 120
           WL+LPHR + +++ FY++ E   + I LPK   G   F  GCSK W++      +  E+ 
Sbjct: 87  WLVLPHRQSCNSLSFYSMAEATVYNIKLPKPAQGGWCF--GCSKSWLMMARGTEWEPEVY 146

Query: 121 LFNPITRVEYFLPS 132
           LFNP +  +  LPS
Sbjct: 147 LFNPFSGFKLNLPS 158

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155350.11.1e-3559.31uncharacterized protein LOC111022492 [Momordica charantia][more]
VVA31268.14.3e-1641.32PREDICTED: F-box/kelch-repeat [Prunus dulcis][more]
KAE8008804.17.4e-1635.93hypothetical protein FH972_005279 [Carpinus fangiana][more]
XP_021818908.13.7e-1539.67uncharacterized protein LOC110760869 [Prunus avium][more]
KAE8008846.14.8e-1534.73hypothetical protein FH972_005319 [Carpinus fangiana][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DM795.3e-3659.31uncharacterized protein LOC111022492 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A5E4FUV82.1e-1641.32PREDICTED: F-box/kelch-repeat OS=Prunus dulcis OX=3755 GN=ALMOND_2B015541 PE=4 S... [more]
A0A5N6QP563.6e-1635.93F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005279 P... [more]
A0A6P5SZ441.8e-1539.67uncharacterized protein LOC110760869 OS=Prunus avium OX=42229 GN=LOC110760869 PE... [more]
A0A5N6R4322.3e-1535.07F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_012527 P... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR44259OS07G0183000 PROTEIN-RELATEDcoord: 13..144
NoneNo IPR availablePANTHERPTHR44259:SF65BNAC03G14360D PROTEINcoord: 13..144
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 7..84

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS022231.1MS022231.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding