Homology
BLAST of MS022200 vs. NCBI nr
Match:
XP_022135713.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])
HSP 1 Score: 698.7 bits (1802), Expect = 2.5e-197
Identity = 344/355 (96.90%), Postives = 348/355 (98.03%), Query Frame = 0
Query: 1 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
MDLALKESTSKKEEDHEHARVQMWNYIFGFTE+AIIKCAIELKIADTIESHGSPMTLS+L
Sbjct: 11 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70
Query: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLT YAQTPLSRLLASNSNNSMAP LLLE
Sbjct: 71 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130
Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
SNPVMLAPWHNLSA IKAKDGTPFEATHGKDLWSYTAADP+HNKIFNDAMACAARVITVP
Sbjct: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190
Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV
Sbjct: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250
Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
EGNMFDCIPKA AAFIMWVLHDWNDEDCIKILKNCRDAIPKK GKVIIVEAVID NEENN
Sbjct: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310
Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAFI 356
L DVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITP+KAVQSVIQAFI
Sbjct: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365
BLAST of MS022200 vs. NCBI nr
Match:
XP_022136086.1 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica charantia])
HSP 1 Score: 667.2 bits (1720), Expect = 8.1e-188
Identity = 325/355 (91.55%), Postives = 342/355 (96.34%), Query Frame = 0
Query: 1 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
MDLALKESTSKKEEDHE A VQMWNYIFGFT+MAIIKCAIELKIADTIESHGSPMTLSQL
Sbjct: 1 MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
Query: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLT YAQTPLSRLLASN NNSMAPLLLLE
Sbjct: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
S+P+MLA WHNLSARIKA DGTPFEATHGKDLWSYTAADP+HN+IFNDAMACAARVITVP
Sbjct: 121 SSPIMLASWHNLSARIKASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVITVP 180
Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWI+GINFDLPHVVSVAQEC+GVQHV
Sbjct: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQHV 240
Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
EGNMFDCIPKA AAF+M VLHDWNDEDCIKILKNC+DA+PKKTGKVIIVE VID NEE N
Sbjct: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEEKN 300
Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAFI 356
LSDVGL+LDMVMMA TNKGKERT EEWA+VLH++GF+RYTITPI+A+QSVIQAFI
Sbjct: 301 LSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 355
BLAST of MS022200 vs. NCBI nr
Match:
XP_038888665.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])
HSP 1 Score: 580.5 bits (1495), Expect = 1.0e-161
Identity = 281/355 (79.15%), Postives = 313/355 (88.17%), Query Frame = 0
Query: 1 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
MD A KES KKEE+ E ARVQ+WNYIFGF E+AI+KCAIELKIADTIE+HGS MTLSQL
Sbjct: 1 MDFAFKESIPKKEEEDEQARVQIWNYIFGFVEIAIVKCAIELKIADTIETHGSSMTLSQL 60
Query: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
S ALNCS LLYRILRFL +RGIFK+E T +NLTSY+QTPLSRLLAS++NNSM PL+LLE
Sbjct: 61 SLALNCSSSLLYRILRFLVNRGIFKQEITEENLTSYSQTPLSRLLASSNNNSMVPLVLLE 120
Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
S+PVMLAPWH LS RIKA +GTPFEA HGKDLWS+ AADP HN I N+AM+C ARV+TVP
Sbjct: 121 SSPVMLAPWHRLSDRIKANEGTPFEAAHGKDLWSFAAADPIHNTIINEAMSCTARVLTVP 180
Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
AILE C EIFEG+GSLVDVGGGNGT LSMIVKACPWI+GIN DLPHV+S +QE GVQHV
Sbjct: 181 AILEDCPEIFEGIGSLVDVGGGNGTCLSMIVKACPWIKGINLDLPHVISSSQEYTGVQHV 240
Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
G+M D IPKA AAF MWVLHDW+DE CIKILKNCRDAIP+KTGKVIIVEAVI+ EENN
Sbjct: 241 SGSMLDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEENN 300
Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAFI 356
LSDVGL+LDMVMMAHTN GKERT++EW YVLH+AGFTRYTITPI+AVQSVIQ F+
Sbjct: 301 LSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITPIRAVQSVIQGFL 355
BLAST of MS022200 vs. NCBI nr
Match:
XP_022952490.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita moschata])
HSP 1 Score: 577.8 bits (1488), Expect = 6.5e-161
Identity = 281/354 (79.38%), Postives = 315/354 (88.98%), Query Frame = 0
Query: 1 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
MD ALK+S SKKEE HEHAR+Q+W YIFGF EMAIIKCAIELKI DTI+SHG MTL +L
Sbjct: 1 MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60
Query: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
SS+LNCS LLYRI+RFL HRGIFKEE T +NLT Y+ TPLSRLLAS+S++SMAPLLLLE
Sbjct: 61 SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120
Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
SNPVMLAPWH LSARIK + PFEA HGKD+WSY AA+P+HN +FNDAMAC+ARV+TVP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTVP 180
Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
AILE CGEIFEGV LVDVGGGNGTSLSMIVKACPWI+GINFDLPHVV+ + +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQHV 240
Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
GNMFDCIPKA AAF+MWVLH W+DE CIKILK CR+AIPKKTGKVIIVEAV++ EENN
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEENN 300
Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
LSDVGL+LDMVMMAHTN GKERT+EEWAYVLH+AGFTR+TITPI+A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353
BLAST of MS022200 vs. NCBI nr
Match:
XP_023554119.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 575.1 bits (1481), Expect = 4.2e-160
Identity = 277/354 (78.25%), Postives = 312/354 (88.14%), Query Frame = 0
Query: 1 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
MD ALK+S SKKEE HEHAR+Q+W YIFGF EMAIIKCAIELKI DTI+SHG MTL +L
Sbjct: 1 MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60
Query: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
SS+LNCS LL+RI+RFL HRGIFK+E T +NLT Y+ TPLSRLL S S NSMAP +LLE
Sbjct: 61 SSSLNCSSSLLHRIMRFLVHRGIFKQEITGENLTRYSHTPLSRLLTSGSENSMAPFVLLE 120
Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
SNPVMLAPWH+LSARIK + PFEA HGKD+WSY AADP HN +FNDAMAC+ARV++VP
Sbjct: 121 SNPVMLAPWHSLSARIKGNEAIPFEAAHGKDIWSYAAADPIHNAMFNDAMACSARVMSVP 180
Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
AILE CGEIFEGV LVDVGGGNGTSLSMIVKACPWI+GINFDLPHV++ +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVIASLPADIGVQHV 240
Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
GNMFDCIPKA AAF+MWVLHDW+DE CIKILKNCRDAIPKKTGKVIIVEAV++ +ENN
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQENN 300
Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
LSDVGL+LDMVMMAHTN GKERT++EWAYVLH+AGFTR+TITPI+A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 353
BLAST of MS022200 vs. ExPASy Swiss-Prot
Match:
Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)
HSP 1 Score: 351.3 bits (900), Expect = 1.3e-95
Identity = 180/367 (49.05%), Postives = 246/367 (67.03%), Query Frame = 0
Query: 5 LKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGS--PMTLSQLSS 64
++++ K ++ A + +W Y+FGF ++A KCAI+LKI + IE+H S P+TL++LSS
Sbjct: 15 MEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSS 74
Query: 65 ALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TSYAQTPLSR--LLASNSNNSMAPLLLL 124
A++ SP L RI+RFL H+GIFKE T L T Y TPLSR ++ S+AP +L
Sbjct: 75 AVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLF 134
Query: 125 ESNPVMLAPWHNL----SARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAAR 184
E+ P MLAPW L S+ + PF+A HGKD+WS+ +P + + N+AMAC AR
Sbjct: 135 ETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR 194
Query: 185 VITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECV 244
+ VP + C +F+GV ++VDVGGG G ++ M+VK PWI+G NFDLPHV+ VA+
Sbjct: 195 RV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLD 254
Query: 245 GVQHVEGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDV 304
GV++VEG+MFD IP A FI WVLHDW D+DCIKILKNC++A+P GKV+IVE+VI
Sbjct: 255 GVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGE 314
Query: 305 NE--------ENNLSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAV 355
N+ + L V L+LDMVMMAHT+ GKERT +EW +VL +AGF RY + I V
Sbjct: 315 NKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDV 374
BLAST of MS022200 vs. ExPASy Swiss-Prot
Match:
Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)
HSP 1 Score: 285.4 bits (729), Expect = 8.7e-76
Identity = 154/351 (43.87%), Postives = 213/351 (60.68%), Query Frame = 0
Query: 14 EDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQLSSALNCSPP---- 73
E+ + ++W ++F F + +KCA+EL I D I SHG P+T+S++ +L + P
Sbjct: 5 EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64
Query: 74 ---LLYRILRFLTHRGIFKEETTAQ-NLTSYAQTPLSRLLASNSNNSMAPLLLLESNPVM 133
L RI+R L H+ +F E + N Y T S+ L +S +++PL+L E+NP++
Sbjct: 65 NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124
Query: 134 LAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVPAILEG 193
L PW L + K +PFE HG ++W ADP N N AM C+ I +LE
Sbjct: 125 LKPWQYLGKCAQEK-SSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLE- 184
Query: 194 CGEIFEGV-GSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNM 253
+ F G+ GSLVDVGGG G+ ++ IVKA P IQGINFDLPHVV+ A E GV+HV G+M
Sbjct: 185 YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDM 244
Query: 254 FDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAI-PKKTGKVIIVEAVIDVNEENNLSD 313
F IP+A A + W+LHDW+DEDC ILKNC AI KK GKVIIV+ V+ + +
Sbjct: 245 FVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDK 304
Query: 314 VGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
+GL+ D++MMAHT GKERT EW +L+ AGF RY + A +I+AF
Sbjct: 305 MGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAF 353
BLAST of MS022200 vs. ExPASy Swiss-Prot
Match:
B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)
HSP 1 Score: 279.3 bits (713), Expect = 6.2e-74
Identity = 161/365 (44.11%), Postives = 222/365 (60.82%), Query Frame = 0
Query: 1 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
M+LA + T E +W I G + ++KCA+EL+I D + SH +P+TL+Q+
Sbjct: 1 MELARNDQT----EAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQI 60
Query: 61 SSALNCSPPL----LYRILRFLTHRGIFKEETTAQ-NLTSYAQTPLSRLLASNS----NN 120
+S++ SP L L RI+R L R IF + + Y T SRLL S +
Sbjct: 61 ASSVPDSPSLNLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQV 120
Query: 121 SMAPLLLLESNPVMLAPWHNLSARIKAKDGTPFEATH-GKDLWSYTAADPSHNKIFNDAM 180
++AP + ++P + APW L AR + G FE H G+ LW + +P NK+FND M
Sbjct: 121 TLAPFVAFMTHPYLSAPWSCL-ARCVKEGGNGFEMVHGGRQLWDLSPGNPEFNKVFNDGM 180
Query: 181 ACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSV 240
A AR+ T+ AIL ++F G+ SLVDVGG G S+S IVK+ P I+GIN+DLPHVV+
Sbjct: 181 ASTARITTM-AILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVAT 240
Query: 241 AQECVG-VQHVEGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIV 300
A G V HV GNMF+ IP A A F+ W+LHDW DEDC+KILKNCR A+P+K GK+IIV
Sbjct: 241 APTYTGLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIV 300
Query: 301 EAVIDVNEENNLSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQS 355
+ V++ D ++LD+ +MA T +GKERT +EW VL + GF RY I I A+ S
Sbjct: 301 DIVLEPEGNGLFDDAAVMLDIALMALT-RGKERTEKEWKRVLEEGGFPRYQILKIPALTS 358
BLAST of MS022200 vs. ExPASy Swiss-Prot
Match:
Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)
HSP 1 Score: 273.5 bits (698), Expect = 3.4e-72
Identity = 142/345 (41.16%), Postives = 219/345 (63.48%), Query Frame = 0
Query: 13 EEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQLSSALNCSP---P 72
++D+ ++ ++WN+I+GF E ++KCA++L +A+ I + G+ MTLS+LSS L P
Sbjct: 4 KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNED 63
Query: 73 LLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLESNPVMLAPW 132
LYR++R+L H +F + + L Y P ++ L + M +L ++ +APW
Sbjct: 64 ALYRVMRYLVHMKLFTKASIDGEL-RYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPW 123
Query: 133 HNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVPAILEGCGEI 192
H L + + GT FE G ++W Y A P N++FN+AMA +R+I + A+++ CG I
Sbjct: 124 HYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLI-MSALVKECGNI 183
Query: 193 FEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNMFDCIP 252
F G+ +LVDVGGG GT++ I A P I+ +DLPHV++ + V V G+MF IP
Sbjct: 184 FNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIP 243
Query: 253 KAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENNLSDVGLLLD 312
KA A + +LHDW+D++CI+ILK C++A+P K GKVIIV+ V++V E+ + + L LD
Sbjct: 244 KADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLD 303
Query: 313 MVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
+ MM +T GKERT EEW ++H AG+ + IT I AVQSVI+A+
Sbjct: 304 LDMMLNTG-GKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAY 345
BLAST of MS022200 vs. ExPASy Swiss-Prot
Match:
Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)
HSP 1 Score: 271.6 bits (693), Expect = 1.3e-71
Identity = 131/339 (38.64%), Postives = 215/339 (63.42%), Query Frame = 0
Query: 20 RVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQLSSAL---NCSPPLLYRILR 79
+ Q+WN I+GF + +++CA+E+ IAD I+++ +TL+QL++ L N S LYR++R
Sbjct: 20 QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVR 79
Query: 80 FLTHRGIFKEETTAQNLTS-YAQTPLSRLLASNSNNSMAPLLLLESNPVMLAPWHNLSAR 139
+L H I ++ET + Y+ P+ LL ++ SM P++L + + WH +
Sbjct: 80 YLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEG 139
Query: 140 IKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVPAILEGCGEIFEGVGS 199
+ T FE G D+W Y +P +++FN+ MA R++T ++E C + F+G+ S
Sbjct: 140 LGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT-KTLIEDCRDTFQGLDS 199
Query: 200 LVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNMFDCIPKAHAAF 259
LVD+GGGNGT++ I +A P I+ +DLPHVV+ + + ++ V G+MF +P A A
Sbjct: 200 LVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAIL 259
Query: 260 IMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENNLSDVGLLLDMVMMAH 319
+ +LHDW DE+C+ ILK C++AIPK+TGKVIIV+ ++ + L+ L+LD+ M+ +
Sbjct: 260 LKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVN 319
Query: 320 TNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
T G+ERT+++W +L +AGF + I PI+A+QSVI+AF
Sbjct: 320 TG-GRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAF 356
BLAST of MS022200 vs. ExPASy TrEMBL
Match:
A0A6J1C5N1 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007607 PE=3 SV=1)
HSP 1 Score: 698.7 bits (1802), Expect = 1.2e-197
Identity = 344/355 (96.90%), Postives = 348/355 (98.03%), Query Frame = 0
Query: 1 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
MDLALKESTSKKEEDHEHARVQMWNYIFGFTE+AIIKCAIELKIADTIESHGSPMTLS+L
Sbjct: 11 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70
Query: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLT YAQTPLSRLLASNSNNSMAP LLLE
Sbjct: 71 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130
Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
SNPVMLAPWHNLSA IKAKDGTPFEATHGKDLWSYTAADP+HNKIFNDAMACAARVITVP
Sbjct: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190
Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV
Sbjct: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250
Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
EGNMFDCIPKA AAFIMWVLHDWNDEDCIKILKNCRDAIPKK GKVIIVEAVID NEENN
Sbjct: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310
Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAFI 356
L DVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITP+KAVQSVIQAFI
Sbjct: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365
BLAST of MS022200 vs. ExPASy TrEMBL
Match:
A0A6J1C2I8 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111007866 PE=3 SV=1)
HSP 1 Score: 667.2 bits (1720), Expect = 3.9e-188
Identity = 325/355 (91.55%), Postives = 342/355 (96.34%), Query Frame = 0
Query: 1 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
MDLALKESTSKKEEDHE A VQMWNYIFGFT+MAIIKCAIELKIADTIESHGSPMTLSQL
Sbjct: 1 MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
Query: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLT YAQTPLSRLLASN NNSMAPLLLLE
Sbjct: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
S+P+MLA WHNLSARIKA DGTPFEATHGKDLWSYTAADP+HN+IFNDAMACAARVITVP
Sbjct: 121 SSPIMLASWHNLSARIKASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVITVP 180
Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWI+GINFDLPHVVSVAQEC+GVQHV
Sbjct: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQHV 240
Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
EGNMFDCIPKA AAF+M VLHDWNDEDCIKILKNC+DA+PKKTGKVIIVE VID NEE N
Sbjct: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEEKN 300
Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAFI 356
LSDVGL+LDMVMMA TNKGKERT EEWA+VLH++GF+RYTITPI+A+QSVIQAFI
Sbjct: 301 LSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 355
BLAST of MS022200 vs. ExPASy TrEMBL
Match:
A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)
HSP 1 Score: 577.8 bits (1488), Expect = 3.1e-161
Identity = 281/354 (79.38%), Postives = 315/354 (88.98%), Query Frame = 0
Query: 1 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
MD ALK+S SKKEE HEHAR+Q+W YIFGF EMAIIKCAIELKI DTI+SHG MTL +L
Sbjct: 1 MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60
Query: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
SS+LNCS LLYRI+RFL HRGIFKEE T +NLT Y+ TPLSRLLAS+S++SMAPLLLLE
Sbjct: 61 SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120
Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
SNPVMLAPWH LSARIK + PFEA HGKD+WSY AA+P+HN +FNDAMAC+ARV+TVP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTVP 180
Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
AILE CGEIFEGV LVDVGGGNGTSLSMIVKACPWI+GINFDLPHVV+ + +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQHV 240
Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
GNMFDCIPKA AAF+MWVLH W+DE CIKILK CR+AIPKKTGKVIIVEAV++ EENN
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEENN 300
Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
LSDVGL+LDMVMMAHTN GKERT+EEWAYVLH+AGFTR+TITPI+A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353
BLAST of MS022200 vs. ExPASy TrEMBL
Match:
A0A6J1GLX9 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455166 PE=3 SV=1)
HSP 1 Score: 559.3 bits (1440), Expect = 1.2e-155
Identity = 274/354 (77.40%), Postives = 308/354 (87.01%), Query Frame = 0
Query: 1 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
MD ALK+S SKKEE HEHAR+Q+W YIFGF EMAIIKCAIELKI DTI+SHG MTL +L
Sbjct: 1 MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60
Query: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
SS+LNCS LLYRI+RFL HRGIFKEE T YAQTP+SRLLAS+S +SMAPLLL E
Sbjct: 61 SSSLNCSSSLLYRIMRFLVHRGIFKEEPT----KGYAQTPMSRLLASSSGSSMAPLLLFE 120
Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
SNPVMLAPWH LSARIK + PFEA HGKD+WSY AA+P+HN +FNDAMAC+ARV++VP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMSVP 180
Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
AILE CGEIFEGV LVDVGGGNGTSLSMIVKACPWI+ INFDLPHV++ + VQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVIASLPADIDVQHV 240
Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
GNMFD IPKA AAF+MWVLHDW+DE CIKILKNCR+AIPKKTGKVIIVEAV++ EENN
Sbjct: 241 GGNMFDSIPKADAAFLMWVLHDWDDETCIKILKNCREAIPKKTGKVIIVEAVLEEKEENN 300
Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
LSDVGL+LDMVMMAHTN GKERT++EWAYVLH+AGFTR+TITPI+A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 349
BLAST of MS022200 vs. ExPASy TrEMBL
Match:
A0A6J1GLV5 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455165 PE=3 SV=1)
HSP 1 Score: 551.6 bits (1420), Expect = 2.4e-153
Identity = 270/354 (76.27%), Postives = 307/354 (86.72%), Query Frame = 0
Query: 2 DLALKESTSK-KEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 61
D+ K+S K +EE++ +VQ+W YIFGF EMA+IKCAIELKI DTIE+HG MTL+ L
Sbjct: 3 DIGSKDSKLKIEEEENNDIKVQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTLTDL 62
Query: 62 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 121
SS+LNCSP LL+RILRFL HRGIFKEE T SYAQTP+SRLLAS+S +SMAPLLL E
Sbjct: 63 SSSLNCSPLLLHRILRFLVHRGIFKEEPT----KSYAQTPMSRLLASSSGSSMAPLLLFE 122
Query: 122 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 181
SNPVMLAPWH+LSARIK + PFEA HGKDLWSY AADP HN +FNDAMAC+ARV++VP
Sbjct: 123 SNPVMLAPWHSLSARIKGNEAIPFEAAHGKDLWSYAAADPIHNTMFNDAMACSARVMSVP 182
Query: 182 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 241
+LE CGEIFEGV LVDVGGGNGTSLSMIVKACPWI+ INFDLPHVV+ + +GVQHV
Sbjct: 183 TLLEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVVASSPPPIGVQHV 242
Query: 242 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 301
GNMFDCIPKA AAF+MWVLHDW+DE CIKILKNCRDAIPKKTGKVIIVEAV++ +ENN
Sbjct: 243 GGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQENN 302
Query: 302 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
LSDVGL+LDMVMMAHTN GKERT++EWAYVLH+AGFTR+TITPI+AVQSVIQAF
Sbjct: 303 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRAVQSVIQAF 352
BLAST of MS022200 vs. TAIR 10
Match:
AT4G35160.1 (O-methyltransferase family protein )
HSP 1 Score: 351.3 bits (900), Expect = 9.2e-97
Identity = 180/367 (49.05%), Postives = 246/367 (67.03%), Query Frame = 0
Query: 5 LKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGS--PMTLSQLSS 64
++++ K ++ A + +W Y+FGF ++A KCAI+LKI + IE+H S P+TL++LSS
Sbjct: 15 MEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSS 74
Query: 65 ALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TSYAQTPLSR--LLASNSNNSMAPLLLL 124
A++ SP L RI+RFL H+GIFKE T L T Y TPLSR ++ S+AP +L
Sbjct: 75 AVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLF 134
Query: 125 ESNPVMLAPWHNL----SARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAAR 184
E+ P MLAPW L S+ + PF+A HGKD+WS+ +P + + N+AMAC AR
Sbjct: 135 ETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR 194
Query: 185 VITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECV 244
+ VP + C +F+GV ++VDVGGG G ++ M+VK PWI+G NFDLPHV+ VA+
Sbjct: 195 RV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLD 254
Query: 245 GVQHVEGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDV 304
GV++VEG+MFD IP A FI WVLHDW D+DCIKILKNC++A+P GKV+IVE+VI
Sbjct: 255 GVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGE 314
Query: 305 NE--------ENNLSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAV 355
N+ + L V L+LDMVMMAHT+ GKERT +EW +VL +AGF RY + I V
Sbjct: 315 NKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDV 374
BLAST of MS022200 vs. TAIR 10
Match:
AT4G35150.1 (O-methyltransferase family protein )
HSP 1 Score: 299.3 bits (765), Expect = 4.1e-81
Identity = 160/361 (44.32%), Postives = 218/361 (60.39%), Query Frame = 0
Query: 5 LKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGS--PMTLSQLSS 64
++ES ++ A + +W Y+FGF ++A KCAI+LKI + IE+H S P+TLS+LSS
Sbjct: 1 MEESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSS 60
Query: 65 ALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TSYAQTPLSRLLASNSNNSMAPLLLLES 124
A++ SP L RI+RFL H+G+FKE T L T Y TPLSR +
Sbjct: 61 AVSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM--------------- 120
Query: 125 NPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVPA 184
HGKDLW++ + H+++ N+AMAC AR + VP
Sbjct: 121 ----------------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPR 180
Query: 185 ILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVE 244
+ C +F+GV ++VDVGGG G ++ ++VK PWI+G NFDLPHV+ VAQ GV++VE
Sbjct: 181 VAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVE 240
Query: 245 GNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNE---- 304
G+MFD IP + A I WVLHDW D+DCIKILKNC++A+ GKV+IVE VI +
Sbjct: 241 GDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMI 300
Query: 305 ----ENNLSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQA 355
++ L V L LDMVMM HT+ GKERT +EW +VL +AGF RY + VQS+I A
Sbjct: 301 AEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIA 323
BLAST of MS022200 vs. TAIR 10
Match:
AT5G54160.1 (O-methyltransferase 1 )
HSP 1 Score: 199.9 bits (507), Expect = 3.4e-51
Identity = 120/329 (36.47%), Postives = 189/329 (57.45%), Query Frame = 0
Query: 36 IKCAIELKIADTIESHGSPMTLSQLSSALNC----SPPLLYRILRFLTHRGIF---KEET 95
+K A+EL + + + +GSPM+ ++++S L +P +L RILR LT + +
Sbjct: 36 LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95
Query: 96 TAQNLTS-YAQTPLSRLLASNSNN-SMAPLLLLESNPVMLAPWHNLSARIKAKDGTPFEA 155
+ + Y P+ + L N + S+A L L+ + V++ W++L I G PF
Sbjct: 96 SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI-LDGGIPFNK 155
Query: 156 THGKDLWSYTAADPSHNKIFNDAMACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTS 215
+G + Y DP NK+FN+ M+ + IT+ ILE + FEG+ SLVDVGGG G +
Sbjct: 156 AYGMSAFEYHGTDPRFNKVFNNGMSNHS-TITMKKILE-TYKGFEGLTSLVDVGGGIGAT 215
Query: 216 LSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNMFDCIPKAHAAFIMWVLHDWNDE 275
L MIV P ++GINFDLPHV+ A G++HV G+MF +PK A F+ W+ HDW+DE
Sbjct: 216 LKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDE 275
Query: 276 DCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENNLSDVGLL-LDMVMMAHTNKGKERTSE 335
C+K LKNC +++P + GKVI+ E ++ +++LS ++ +D +M+AH GKERT +
Sbjct: 276 HCVKFLKNCYESLP-EDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEK 335
Query: 336 EWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
E+ + +GF K ++ V AF
Sbjct: 336 EFEALAKASGF--------KGIKVVCDAF 352
BLAST of MS022200 vs. TAIR 10
Match:
AT1G77530.1 (O-methyltransferase family protein )
HSP 1 Score: 180.3 bits (456), Expect = 2.8e-45
Identity = 125/363 (34.44%), Postives = 199/363 (54.82%), Query Frame = 0
Query: 11 KKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTI--ESHGSPMTLSQLSSALNC-- 70
++E D + +Q + + ++K A+EL + DTI S+G+ ++ S+++ +L
Sbjct: 21 EQEIDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKP 80
Query: 71 ----SPPLLYRILRFLTHRGIFK--------EETTAQNLTSYAQTPLSR--LLASNSNNS 130
+P LL R+LR L I K T + YA P+ + L S+ + S
Sbjct: 81 TNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGS 140
Query: 131 MAPLLLLESNPVMLAPWHNL-SARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMA 190
++ LLLL + V+L W NL ++ KD F + H L+ Y ++D +K+F+ AM+
Sbjct: 141 LSSLLLLLHSQVILKTWTNLKDVILEGKDA--FSSAHDMRLFEYISSDDQFSKLFHRAMS 200
Query: 191 CAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVA 250
++ ++ + E G FE V +LVDVGGG GT L +I P I+G+NFDL V++ A
Sbjct: 201 ESSTMVMKKVLEEYRG--FEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQA 260
Query: 251 QECVGVQHVEGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEA 310
GV+HV G+MF +PK A F+ W+LHDW DEDCIKILKNC ++P+K GKVIIVE
Sbjct: 261 PFYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEK-GKVIIVEM 320
Query: 311 VIDVNEENN--LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQS 353
+ + + N + L +D++M+ + GKER+ ++ + +GF I + S
Sbjct: 321 ITPMEPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYS 378
BLAST of MS022200 vs. TAIR 10
Match:
AT1G77520.1 (O-methyltransferase family protein )
HSP 1 Score: 176.8 bits (447), Expect = 3.1e-44
Identity = 123/363 (33.88%), Postives = 185/363 (50.96%), Query Frame = 0
Query: 11 KKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQLSSALNC---- 70
++E D + +Q + + ++K A EL + DTI + G+ LS A +
Sbjct: 21 EQEVDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKP 80
Query: 71 ----SPPLLYRILRFLTHRGIFK---EETTAQNLTS-----YAQTPLSR--LLASNSNNS 130
+P LL R+L L I K ET T YA P+ + L S+ S
Sbjct: 81 TNPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGS 140
Query: 131 MAPLLLLESNPVMLAPWHNL-SARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMA 190
+ PL +L V W NL ++ +D F + HG ++ Y +D ++FN AM+
Sbjct: 141 LVPLFMLLHTQVFFKTWTNLKDVILEGRDA--FNSAHGMKIFEYINSDQPFAELFNRAMS 200
Query: 191 CAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVA 250
+ +I + G FE V +LVDVGGGNGT L ++ P I+G+NFDL V++ A
Sbjct: 201 EPSTMIMKKVLDVYRG--FEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQA 260
Query: 251 QECVGVQHVEGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEA 310
GV+HV G+MF +PK A F+ W+LHDW DEDCIKILKNC ++P+K GK+IIVE
Sbjct: 261 PFYPGVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEF 320
Query: 311 VIDVNEENN--LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQS 353
V + S+ +D++M+ + GKER+ ++ + +GF R I + S
Sbjct: 321 VTPKEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYS 378
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022135713.1 | 2.5e-197 | 96.90 | (R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia] | [more] |
| XP_022136086.1 | 8.1e-188 | 91.55 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica char... | [more] |
| XP_038888665.1 | 1.0e-161 | 79.15 | acetylserotonin O-methyltransferase-like [Benincasa hispida] | [more] |
| XP_022952490.1 | 6.5e-161 | 79.38 | (RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita moschata] | [more] |
| XP_023554119.1 | 4.2e-160 | 78.25 | (RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9T003 | 1.3e-95 | 49.05 | Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1... | [more] |
| Q6WUC2 | 8.7e-76 | 43.87 | (R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=... | [more] |
| B0ZB56 | 6.2e-74 | 44.11 | Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1 | [more] |
| Q9LEL6 | 3.4e-72 | 41.16 | (RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1 | [more] |
| Q7XB10 | 1.3e-71 | 38.64 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferu... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1C5N1 | 1.2e-197 | 96.90 | (R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO... | [more] |
| A0A6J1C2I8 | 3.9e-188 | 91.55 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica ch... | [more] |
| A0A6J1GLX2 | 3.1e-161 | 79.38 | (RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L... | [more] |
| A0A6J1GLX9 | 1.2e-155 | 77.40 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Cucurbita mo... | [more] |
| A0A6J1GLV5 | 2.4e-153 | 76.27 | (RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L... | [more] |
