MS022198 (gene) Bitter gourd (TR) v1

BLAST of MS022198 vs. NCBI nr
Match: XP_022135685.1 (desmethylxanthohumol 6'-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 722.6 bits (1864), Expect = 1.7e-204
Identity = 354/355 (99.72%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 11  MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 70
           MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL
Sbjct: 1   MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 60

Query: 71  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 130
           SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE
Sbjct: 61  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 120

Query: 131 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 190
           NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV
Sbjct: 121 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 180

Query: 191 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQH 250
           PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENV+H
Sbjct: 181 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVRH 240

Query: 251 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 310
           VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS
Sbjct: 241 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 300

Query: 311 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
           KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF
Sbjct: 301 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 355

BLAST of MS022198 vs. NCBI nr
Match: XP_022136173.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 621.7 bits (1602), Expect = 4.0e-174
Identity = 303/355 (85.35%), Postives = 329/355 (92.68%), Query Frame = 0

Query: 11  MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 70
           ME+SHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL
Sbjct: 1   MENSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 60

Query: 71  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 130
           SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESS+APLFLLE
Sbjct: 61  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSVAPLFLLE 120

Query: 131 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 190
           NGP MLAPWHNL A IK  S+ LPFEAAHG+DLW YAAANPT+N LF+EA+AC  R+I V
Sbjct: 121 NGPAMLAPWHNLCAKIKGVSDVLPFEAAHGSDLWGYAAANPTYNTLFHEAMACGARLIHV 180

Query: 191 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQH 250
           PAILE+CGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRG+NFDLPHVV+ SPEYENVQH
Sbjct: 181 PAILETCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVAASPEYENVQH 240

Query: 251 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 310
           VGG+MF+YVPKADAAF  WILH WDDEECIKILKNCKEAIPK  GKVIIVE VIDEKE+S
Sbjct: 241 VGGSMFNYVPKADAAFLKWILHDWDDEECIKILKNCKEAIPKDTGKVIIVEAVIDEKEES 300

Query: 311 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
           + SD+R+M+DM +MA  K G+ERTA+E A++LN+AGFSRHTI  IGG+PS+IQAF
Sbjct: 301 EVSDVRVMVDMVVMAHTKNGRERTAEELAHVLNKAGFSRHTIISIGGVPSVIQAF 355

BLAST of MS022198 vs. NCBI nr
Match: XP_022952490.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 520.8 bits (1340), Expect = 9.7e-144
Identity = 251/355 (70.70%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 11  MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 70
           M+ + K+S  KKEE +  A +Q+W++IFGF E+A++KCAIELKIGD I+ +G  MTL +L
Sbjct: 1   MDFALKQSISKKEEHE-HARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 71  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 130
           SS+LNCSS LLYRI+RFL+ RGIF+EE T ENLT Y+ TPLSRLL +S +SSMAPL LLE
Sbjct: 61  SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120

Query: 131 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 190
           + PVMLAPWH LSA IK N E +PFEAAHG D+WSYAAANPT NA+FN+A+AC  RV+TV
Sbjct: 121 SNPVMLAPWHGLSARIKGN-EAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTV 180

Query: 191 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQH 250
           PAILE CGE+FEGV CLVDVGGGNGTSLSMIVKACPWI+G+NFDLPHVV++SP Y  VQH
Sbjct: 181 PAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQH 240

Query: 251 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 310
           VGGNMFD +PKADAAF MW+LH WDDE CIKILK C+EAIPK  GKVIIVE V++EKE++
Sbjct: 241 VGGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEEN 300

Query: 311 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
             SD+ LMLDM MMA    GKERTA+EWAY+L++AGF+RHTITPI  L S+IQAF
Sbjct: 301 NLSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353

BLAST of MS022198 vs. NCBI nr
Match: KAG6572140.1 (Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 517.3 bits (1331), Expect = 1.1e-142
Identity = 250/355 (70.42%), Postives = 296/355 (83.38%), Query Frame = 0

Query: 11  MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 70
           M+ + K+S  KKEE +  A +Q+W++IFGF E+A++KCAIELKIGD I+ +G  MTL +L
Sbjct: 1   MDFALKQSISKKEEHE-HARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 71  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 130
           SS+LNCSS LLYRI+RFL+ RGIF+EE T ENLT Y+ TPLSRLL +S +SSMAPL LLE
Sbjct: 61  SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120

Query: 131 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 190
           + PVMLAPWH LSA IK N E +PFEAAHG D+WSYAAANPT NA+FN+A+AC  RV+TV
Sbjct: 121 SNPVMLAPWHGLSARIKGN-EAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTV 180

Query: 191 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQH 250
           PAILE CGE+FEGV CLVDVGGGNGTSLSMIVKACPWI+ +NFDLPHVV++SP Y  VQH
Sbjct: 181 PAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVVASSPPYIGVQH 240

Query: 251 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 310
           VGGNMFD +PKADAAF MW+LH WDDE CIKILK C+EAIPK  GKVIIVE V++EKE++
Sbjct: 241 VGGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEEN 300

Query: 311 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
             SD+ LMLDM MMA    GKERTA EWAY+L++AGF+RHTITPI  L S+IQAF
Sbjct: 301 NLSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 353

BLAST of MS022198 vs. NCBI nr
Match: XP_022136086.1 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica charantia])

HSP 1 Score: 511.1 bits (1315), Expect = 7.7e-141
Identity = 249/355 (70.14%), Postives = 293/355 (82.54%), Query Frame = 0

Query: 11  MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 70
           M+ + KES  KKEE   +A +Q+W +IFGF+++A++KCAIELKI D IE +GSPMTLSQL
Sbjct: 1   MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60

Query: 71  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 130
           SSALNCS PLLYRILRFL  RGIF+EETT +NLT YAQTPLSRLL ++G +SMAPL LLE
Sbjct: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120

Query: 131 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 190
           + P+MLA WHNLSA IK  S+  PFEA HG DLWSY AA+PT N +FN+A+AC  RVITV
Sbjct: 121 SSPIMLASWHNLSARIK-ASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVITV 180

Query: 191 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQH 250
           PAILE CGE+FEGVG LVDVGGGNGTSLSMIVKACPWIRG+NFDLPHVVS + E   VQH
Sbjct: 181 PAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQH 240

Query: 251 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 310
           V GNMFD +PKADAAF M +LH W+DE+CIKILKNCK+A+PK  GKVIIVE VID  E+ 
Sbjct: 241 VEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEEK 300

Query: 311 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
             SD+ LMLDM MMA+  KGKERT +EWA++L+E+GFSR+TITPI  + S+IQAF
Sbjct: 301 NLSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAF 354

BLAST of MS022198 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.9e-90
Identity = 174/366 (47.54%), Postives = 244/366 (66.67%), Query Frame = 0

Query: 16  KESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGS--PMTLSQLSSA 75
           +++K K  + +++A++ IWK++FGF++IA  KCAI+LKI +AIE + S  P+TL++LSSA
Sbjct: 16  EDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSA 75

Query: 76  LNCSSPLLYRILRFLIRRGIFREETTPENL-TAYAQTPLSR--LLTTSGESSMAPLFLLE 135
           ++ S   L RI+RFL+ +GIF+E  T + L T Y  TPLSR  ++T     S+AP  L E
Sbjct: 76  VSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFE 135

Query: 136 NGPVMLAPWHNLSANIK---NNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRV 195
             P MLAPW  LS+ +    N S   PF+A HG D+WS+A  NP  + + NEA+AC  R 
Sbjct: 136 TTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARR 195

Query: 196 ITVPAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYEN 255
           + VP +  +C  +F+GV  +VDVGGG G ++ M+VK  PWI+G NFDLPHV+  +   + 
Sbjct: 196 V-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDG 255

Query: 256 VQHVGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEK 315
           V++V G+MFD +P  DA F  W+LH W D++CIKILKNCKEA+P + GKV+IVE VI E 
Sbjct: 256 VENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGEN 315

Query: 316 EKS--------KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLP 366
           +K+        K   +RLMLDM MMA    GKERT  EW ++L EAGF+R+ +  I  + 
Sbjct: 316 KKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQ 375

BLAST of MS022198 vs. ExPASy Swiss-Prot
Match: B0ZB55 (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=2)

HSP 1 Score: 276.2 bits (705), Expect = 5.4e-73
Identity = 148/349 (42.41%), Postives = 223/349 (63.90%), Query Frame = 0

Query: 32  QIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQLSSALNCSS--------PLLYR 91
           QIW+ +F F +   +KCAIEL+I D I  +G P+TLSQ++S +  +S        P L R
Sbjct: 9   QIWQLMFSFVDSMALKCAIELRIADIIHSHGKPITLSQIASGIRSNSNSSISPNIPYLSR 68

Query: 92  ILRFLIRRGIFRE--ETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLENGPVMLAPWHN 151
           I+RFL+R+ IF E  E   E ++ Y  +  SR L    +SS+AP+ L++  P+ +A WH 
Sbjct: 69  IMRFLVRKNIFTEHQEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPLSMAVWHF 128

Query: 152 LSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITVPAILESCGEVF 211
           L   ++N+S    FE AHG ++W +++ANP FN +FN A+A     I + A+L S     
Sbjct: 129 LEDYVRNSSN--TFEKAHGCNIWEFSSANPDFNKIFNNAMASI-VPIYMGAVLSS---YK 188

Query: 212 EGVGCL----VDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQHVGGNMFD 271
           +G+GC+    VDVGGG G S+S ++K  P I+G+NFDLPHV++T+P  + V H+ G++F+
Sbjct: 189 DGLGCIKGTVVDVGGGTGGSISELMKYYPNIKGINFDLPHVIATAPALDGVTHISGDIFE 248

Query: 272 YVPKADAAFFMWILHCWDDEECIKILKNCKEAI-PKSRGKVIIVEIVIDEKEKSKTSDMR 331
            +P ADA     +LHC+ DE+C+K+L+NC++AI  K  GK+II+EIV+D        + R
Sbjct: 249 SIPSADAVLMKGVLHCFSDEKCVKVLRNCRKAITDKKNGKIIILEIVLDPTSNQIFDETR 308

Query: 332 LMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
           ++ D+ ++  F  GKERT  EW  LLNEAGF+   IT I  +P++I+AF
Sbjct: 309 MVYDL-LIPLFSGGKERTELEWKRLLNEAGFTSIKITKIPIIPAIIEAF 350

BLAST of MS022198 vs. ExPASy Swiss-Prot
Match: Q6WUC1 ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 6.6e-71
Identity = 145/345 (42.03%), Postives = 210/345 (60.87%), Query Frame = 0

Query: 26  DSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQLSSALNCSSPL----L 85
           D +   +IWK I+GF+E  V+KCA++L+I + +     PM+LS+L+S L  + P+    L
Sbjct: 8   DQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNEDRL 67

Query: 86  YRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLENGPVMLAPWHN 145
           +RI+R+L+   +F+ + T +    Y+  P ++ L    E SM    L  N    LAPWH+
Sbjct: 68  FRIMRYLVHMELFKIDATTQK---YSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWHH 127

Query: 146 LSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITVPAILESCGEVF 205
           L   +  N +   FE A G  +W Y + NP  N LFN A+AC  R++T  A+   C  +F
Sbjct: 128 LGDGLTGNCD--AFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVT-SALANECKSIF 187

Query: 206 -EGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQHVGGNMFDYVP 265
            +G+  LVDVGGG GT++  I KA P I+   +DLPHV++ SPE  N+  + G+MF  +P
Sbjct: 188 SDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIP 247

Query: 266 KADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKSKTSDMRLMLD 325
            ADA F   ILH W+D+ECI+ILK CKEA+PK  GKVIIV++VID       + +RL LD
Sbjct: 248 SADAIFMKCILHDWNDDECIQILKRCKEALPKG-GKVIIVDVVIDMDSTHPYAKIRLTLD 307

Query: 326 MAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
           + MM     GKERT +EW  L + AGF+ H +T I  + S+I+A+
Sbjct: 308 LDMMLN-TGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAY 344

BLAST of MS022198 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.5e-70
Identity = 149/344 (43.31%), Postives = 212/344 (61.63%), Query Frame = 0

Query: 33  IWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQLSSALNCSSPL----LYRILRFL 92
           +WK I G ++  V+KCA+EL+I D +  + +P+TL+Q++S++  S  L    L RI+R L
Sbjct: 19  VWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSVPDSPSLNLSYLSRIMRLL 78

Query: 93  IRRGIFREETTPE-NLTAYAQTPLSRLL----TTSGESSMAPLFLLENGPVMLAPWHNLS 152
           +RR IF +  + +     Y  T  SRLL    T   + ++AP       P + APW  L+
Sbjct: 79  VRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVAFMTHPYLSAPWSCLA 138

Query: 153 ANIKNNSEWLPFEAAH-GNDLWSYAAANPTFNALFNEAIACFGRVITVPAILESCGEVFE 212
             +K       FE  H G  LW  +  NP FN +FN+ +A   R+ T+ AIL    +VF 
Sbjct: 139 RCVKEGGN--GFEMVHGGRQLWDLSPGNPEFNKVFNDGMASTARITTM-AILSEYRDVFC 198

Query: 213 GVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYEN-VQHVGGNMFDYVPK 272
           G+  LVDVGG  G S+S IVK+ P I+G+N+DLPHVV+T+P Y   V HVGGNMF+++P 
Sbjct: 199 GICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVATAPTYTGLVSHVGGNMFEWIPT 258

Query: 273 ADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKSKTSDMRLMLDM 332
           A A F  WILH W DE+C+KILKNC+ A+P+  GK+IIV+IV++ +      D  +MLD+
Sbjct: 259 AVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIVDIVLEPEGNGLFDDAAVMLDI 318

Query: 333 AMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
           A+MA   +GKERT  EW  +L E GF R+ I  I  L S+I+A+
Sbjct: 319 ALMA-LTRGKERTEKEWKRVLEEGGFPRYQILKIPALTSVIEAY 358

BLAST of MS022198 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 3.6e-69
Identity = 146/344 (42.44%), Postives = 201/344 (58.43%), Query Frame = 0

Query: 32  QIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQLSSALNCSSP-------LLYRI 91
           +IW+H+F F +   +KCA+EL I D I  +G P+T+S++  +L  ++P        L RI
Sbjct: 13  EIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSPNIDYLTRI 72

Query: 92  LRFLIRRGIFREETTPE-NLTAYAQTPLSRLLTTSGESSMAPLFLLENGPVMLAPWHNLS 151
           +R L+ + +F  E   E N   Y  T  S+ L    + +++PL L E  P++L PW  L 
Sbjct: 73  MRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPILLKPWQYLG 132

Query: 152 ANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITVPAILESCGEVFEG 211
              +  S   PFE AHG ++W  A A+P FN   N A+ C    I    +LE   + F G
Sbjct: 133 KCAQEKSS--PFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLE-YKDGFSG 192

Query: 212 V-GCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQHVGGNMFDYVPKA 271
           + G LVDVGGG G+ ++ IVKA P I+G+NFDLPHVV+T+ E+  V+HVGG+MF  +P+A
Sbjct: 193 IAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDMFVDIPEA 252

Query: 272 DAAFFMWILHCWDDEECIKILKNCKEAIPKSR-GKVIIVEIVIDEKEKSKTSDMRLMLDM 331
           DA    WILH W DE+C  ILKNC  AI K + GKVIIV+ V+          M L+ D+
Sbjct: 253 DAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDKMGLIFDV 312

Query: 332 AMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
            MMA    GKERT  EW  LLN AGF R+ +      P +I+AF
Sbjct: 313 LMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAF 353

BLAST of MS022198 vs. ExPASy TrEMBL
Match: A0A6J1C1Q8 (desmethylxanthohumol 6'-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007583 PE=3 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 8.1e-205
Identity = 354/355 (99.72%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 11  MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 70
           MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL
Sbjct: 1   MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 60

Query: 71  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 130
           SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE
Sbjct: 61  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 120

Query: 131 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 190
           NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV
Sbjct: 121 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 180

Query: 191 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQH 250
           PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENV+H
Sbjct: 181 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVRH 240

Query: 251 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 310
           VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS
Sbjct: 241 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 300

Query: 311 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
           KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF
Sbjct: 301 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 355

BLAST of MS022198 vs. ExPASy TrEMBL
Match: A0A6J1C6X0 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007930 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.9e-174
Identity = 303/355 (85.35%), Postives = 329/355 (92.68%), Query Frame = 0

Query: 11  MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 70
           ME+SHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL
Sbjct: 1   MENSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 60

Query: 71  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 130
           SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESS+APLFLLE
Sbjct: 61  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSVAPLFLLE 120

Query: 131 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 190
           NGP MLAPWHNL A IK  S+ LPFEAAHG+DLW YAAANPT+N LF+EA+AC  R+I V
Sbjct: 121 NGPAMLAPWHNLCAKIKGVSDVLPFEAAHGSDLWGYAAANPTYNTLFHEAMACGARLIHV 180

Query: 191 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQH 250
           PAILE+CGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRG+NFDLPHVV+ SPEYENVQH
Sbjct: 181 PAILETCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVAASPEYENVQH 240

Query: 251 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 310
           VGG+MF+YVPKADAAF  WILH WDDEECIKILKNCKEAIPK  GKVIIVE VIDEKE+S
Sbjct: 241 VGGSMFNYVPKADAAFLKWILHDWDDEECIKILKNCKEAIPKDTGKVIIVEAVIDEKEES 300

Query: 311 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
           + SD+R+M+DM +MA  K G+ERTA+E A++LN+AGFSRHTI  IGG+PS+IQAF
Sbjct: 301 EVSDVRVMVDMVVMAHTKNGRERTAEELAHVLNKAGFSRHTIISIGGVPSVIQAF 355

BLAST of MS022198 vs. ExPASy TrEMBL
Match: A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 4.7e-144
Identity = 251/355 (70.70%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 11  MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 70
           M+ + K+S  KKEE +  A +Q+W++IFGF E+A++KCAIELKIGD I+ +G  MTL +L
Sbjct: 1   MDFALKQSISKKEEHE-HARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 71  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 130
           SS+LNCSS LLYRI+RFL+ RGIF+EE T ENLT Y+ TPLSRLL +S +SSMAPL LLE
Sbjct: 61  SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120

Query: 131 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 190
           + PVMLAPWH LSA IK N E +PFEAAHG D+WSYAAANPT NA+FN+A+AC  RV+TV
Sbjct: 121 SNPVMLAPWHGLSARIKGN-EAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTV 180

Query: 191 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQH 250
           PAILE CGE+FEGV CLVDVGGGNGTSLSMIVKACPWI+G+NFDLPHVV++SP Y  VQH
Sbjct: 181 PAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQH 240

Query: 251 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 310
           VGGNMFD +PKADAAF MW+LH WDDE CIKILK C+EAIPK  GKVIIVE V++EKE++
Sbjct: 241 VGGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEEN 300

Query: 311 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
             SD+ LMLDM MMA    GKERTA+EWAY+L++AGF+RHTITPI  L S+IQAF
Sbjct: 301 NLSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353

BLAST of MS022198 vs. ExPASy TrEMBL
Match: A0A6J1C2I8 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111007866 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.7e-141
Identity = 249/355 (70.14%), Postives = 293/355 (82.54%), Query Frame = 0

Query: 11  MESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSPMTLSQL 70
           M+ + KES  KKEE   +A +Q+W +IFGF+++A++KCAIELKI D IE +GSPMTLSQL
Sbjct: 1   MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60

Query: 71  SSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMAPLFLLE 130
           SSALNCS PLLYRILRFL  RGIF+EETT +NLT YAQTPLSRLL ++G +SMAPL LLE
Sbjct: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120

Query: 131 NGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITV 190
           + P+MLA WHNLSA IK  S+  PFEA HG DLWSY AA+PT N +FN+A+AC  RVITV
Sbjct: 121 SSPIMLASWHNLSARIK-ASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVITV 180

Query: 191 PAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQH 250
           PAILE CGE+FEGVG LVDVGGGNGTSLSMIVKACPWIRG+NFDLPHVVS + E   VQH
Sbjct: 181 PAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQH 240

Query: 251 VGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS 310
           V GNMFD +PKADAAF M +LH W+DE+CIKILKNCK+A+PK  GKVIIVE VID  E+ 
Sbjct: 241 VEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEEK 300

Query: 311 KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQAF 366
             SD+ LMLDM MMA+  KGKERT +EWA++L+E+GFSR+TITPI  + S+IQAF
Sbjct: 301 NLSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAF 354

BLAST of MS022198 vs. ExPASy TrEMBL
Match: A0A6J1C5N1 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007607 PE=3 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 4.1e-140
Identity = 246/361 (68.14%), Postives = 294/361 (81.44%), Query Frame = 0

Query: 5   KIHNITMESSHKESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGSP 64
           K+  + M+ + KES  KKEE    A +Q+W +IFGF+E+A++KCAIELKI D IE +GSP
Sbjct: 5   KLIALIMDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSP 64

Query: 65  MTLSQLSSALNCSSPLLYRILRFLIRRGIFREETTPENLTAYAQTPLSRLLTTSGESSMA 124
           MTLS+LSSALNCS PLLYRILRFL  RGIF+EETT +NLT YAQTPLSRLL ++  +SMA
Sbjct: 65  MTLSKLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMA 124

Query: 125 PLFLLENGPVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACF 184
           P  LLE+ PVMLAPWHNLSA IK   +  PFEA HG DLWSY AA+PT N +FN+A+AC 
Sbjct: 125 PFLLLESNPVMLAPWHNLSALIK-AKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACA 184

Query: 185 GRVITVPAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPE 244
            RVITVPAILE CGE+FEGVG LVDVGGGNGTSLSMIVKACPWI+G+NFDLPHVVS + E
Sbjct: 185 ARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQE 244

Query: 245 YENVQHVGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVI 304
              VQHV GNMFD +PKADAAF MW+LH W+DE+CIKILKNC++AIPK  GKVIIVE VI
Sbjct: 245 CVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVI 304

Query: 305 DEKEKSKTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQA 364
           D  E++   D+ L+LDM MMA   KGKERT++EWAY+L++AGF+R+TITP+  + S+IQA
Sbjct: 305 DGNEENNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQA 364

Query: 365 F 366
           F
Sbjct: 365 F 364

BLAST of MS022198 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 333.6 bits (854), Expect = 2.0e-91
Identity = 174/366 (47.54%), Postives = 244/366 (66.67%), Query Frame = 0

Query: 16  KESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGS--PMTLSQLSSA 75
           +++K K  + +++A++ IWK++FGF++IA  KCAI+LKI +AIE + S  P+TL++LSSA
Sbjct: 16  EDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSA 75

Query: 76  LNCSSPLLYRILRFLIRRGIFREETTPENL-TAYAQTPLSR--LLTTSGESSMAPLFLLE 135
           ++ S   L RI+RFL+ +GIF+E  T + L T Y  TPLSR  ++T     S+AP  L E
Sbjct: 76  VSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFE 135

Query: 136 NGPVMLAPWHNLSANIK---NNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRV 195
             P MLAPW  LS+ +    N S   PF+A HG D+WS+A  NP  + + NEA+AC  R 
Sbjct: 136 TTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARR 195

Query: 196 ITVPAILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYEN 255
           + VP +  +C  +F+GV  +VDVGGG G ++ M+VK  PWI+G NFDLPHV+  +   + 
Sbjct: 196 V-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDG 255

Query: 256 VQHVGGNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEK 315
           V++V G+MFD +P  DA F  W+LH W D++CIKILKNCKEA+P + GKV+IVE VI E 
Sbjct: 256 VENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGEN 315

Query: 316 EKS--------KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLP 366
           +K+        K   +RLMLDM MMA    GKERT  EW ++L EAGF+R+ +  I  + 
Sbjct: 316 KKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQ 375

BLAST of MS022198 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 275.0 bits (702), Expect = 8.6e-74
Identity = 149/361 (41.27%), Postives = 215/361 (59.56%), Query Frame = 0

Query: 16  KESKLKKEEGDSEATIQIWKHIFGFSEIAVVKCAIELKIGDAIECYGS--PMTLSQLSSA 75
           +ESK    + +++A++ IW+++FGF++IA  KCAI+LKI +AIE + S  P+TLS+LSSA
Sbjct: 2   EESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSA 61

Query: 76  LNCSSPLLYRILRFLIRRGIFREETTPENL-TAYAQTPLSRLLTTSGESSMAPLFLLENG 135
           ++ S   L RI+RFL+ +G+F+E  T + L T Y  TPLSR +  +              
Sbjct: 62  VSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMMIT-------------- 121

Query: 136 PVMLAPWHNLSANIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITVPA 195
                                     HG DLW++A  N   + L NEA+AC  R + VP 
Sbjct: 122 ------------------------KLHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPR 181

Query: 196 ILESCGEVFEGVGCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQHVG 255
           +  +C  +F+GV  +VDVGGG G ++ ++VK  PWI+G NFDLPHV+  +   + V++V 
Sbjct: 182 VAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVE 241

Query: 256 GNMFDYVPKADAAFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKS-- 315
           G+MFD +P +DA    W+LH W D++CIKILKNCKEA+  + GKV+IVE VI EK+ +  
Sbjct: 242 GDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMI 301

Query: 316 ------KTSDMRLMLDMAMMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQA 366
                 K   +RL LDM MM     GKERT  EW ++L EAGF+R+ +     + SLI A
Sbjct: 302 AEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIA 323

BLAST of MS022198 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-48
Identity = 116/312 (37.18%), Postives = 177/312 (56.73%), Query Frame = 0

Query: 46  VKCAIELKIGDAIECYGSPMTLSQLSSALNCSSP----LLYRILRFLIRRGIF----REE 105
           +K A+EL + + +   GSPM+ ++++S L   +P    +L RILR L    +     R+ 
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95

Query: 106 TTPENLTAYAQTPLSRLLTTSGES-SMAPLFLLENGPVMLAPWHNLSANIKNNSEWLPFE 165
           +       Y   P+ + LT + +  S+A L L+    V++  W++L   I +    +PF 
Sbjct: 96  SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGG--IPFN 155

Query: 166 AAHGNDLWSYAAANPTFNALFNEAIACFGRVITVPAILESCGEVFEGVGCLVDVGGGNGT 225
            A+G   + Y   +P FN +FN  ++     IT+  ILE+  + FEG+  LVDVGGG G 
Sbjct: 156 KAYGMSAFEYHGTDPRFNKVFNNGMSNHS-TITMKKILETY-KGFEGLTSLVDVGGGIGA 215

Query: 226 SLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQHVGGNMFDYVPKADAAFFMWILHCWDD 285
           +L MIV   P ++G+NFDLPHV+  +P +  ++HVGG+MF  VPK DA F  WI H W D
Sbjct: 216 TLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSD 275

Query: 286 EECIKILKNCKEAIPKSRGKVIIVEIVIDEKEKSKTSDMRLM-LDMAMMARFKKGKERTA 345
           E C+K LKNC E++P+  GKVI+ E ++ E   S  S  +++ +D  M+A    GKERT 
Sbjct: 276 EHCVKFLKNCYESLPED-GKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTE 335

Query: 346 DEWAYLLNEAGF 348
            E+  L   +GF
Sbjct: 336 KEFEALAKASGF 342

BLAST of MS022198 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.4e-44
Identity = 125/341 (36.66%), Postives = 177/341 (51.91%), Query Frame = 0

Query: 45  VVKCAIELKIGDAIECYGSPMTLSQLSSALNCSSP----------LLYRILRFLIRRGIF 104
           V+K A EL + D I   G+   LS    A  CS P          LL R+L  L+   I 
Sbjct: 45  VLKAAFELGVIDTIAAAGNDTWLSPCEIA--CSLPTKPTNPEAPVLLDRMLSLLVSHSIL 104

Query: 105 --REETTPEN------LTAYAQTPLSR--LLTTSGESSMAPLFLLENGPVMLAPWHNLSA 164
             R   T EN         YA  P+ +  L  + G  S+ PLF+L +  V    W NL  
Sbjct: 105 KCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQVFFKTWTNLKD 164

Query: 165 NIKNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITVPAILESCGEVFEGV 224
            I    +   F +AHG  ++ Y  ++  F  LFN A++    +I    +    G  FE V
Sbjct: 165 VILEGRD--AFNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVLDVYRG--FEDV 224

Query: 225 GCLVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQHVGGNMFDYVPKADA 284
             LVDVGGGNGT L ++    P I+G+NFDL  V++ +P Y  V+HV G+MF  VPK DA
Sbjct: 225 NTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDA 284

Query: 285 AFFMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIVIDEKEK--SKTSDMRLMLDMA 344
            F  WILH W DE+CIKILKNC +++P+ +GK+IIVE V  ++ K    +S+    +D+ 
Sbjct: 285 VFMKWILHDWGDEDCIKILKNCWKSLPE-KGKIIIVEFVTPKEPKGGDLSSNTVFAMDLL 344

Query: 345 MMARFKKGKERTADEWAYLLNEAGFSRHTITPIGGLPSLIQ 364
           M+ +   GKER+  ++  L   +GF R  I  +    S+I+
Sbjct: 345 MLTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVIE 378

BLAST of MS022198 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.3e-42
Identity = 116/323 (35.91%), Postives = 179/323 (55.42%), Query Frame = 0

Query: 45  VVKCAIELKIGDAIEC--YGSPMTLSQLSSAL-----NCSSP-LLYRILRFLIRRGIFR- 104
           V+K A+EL + D I     G+ ++ S+++ +L     N  +P LL R+LR L+   I + 
Sbjct: 45  VLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILKC 104

Query: 105 -------EETTPENLTAYAQTPLSR--LLTTSGESSMAPLFLLENGPVMLAPWHNLSANI 164
                     T +    YA  P+ +  L  + G  S++ L LL +  V+L  W NL   I
Sbjct: 105 CMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSSLLLLLHSQVILKTWTNLKDVI 164

Query: 165 KNNSEWLPFEAAHGNDLWSYAAANPTFNALFNEAIACFGRVITVPAILESCGEVFEGVGC 224
               +   F +AH   L+ Y +++  F+ LF+ A++    ++    + E  G  FE V  
Sbjct: 165 LEGKD--AFSSAHDMRLFEYISSDDQFSKLFHRAMSESSTMVMKKVLEEYRG--FEDVNT 224

Query: 225 LVDVGGGNGTSLSMIVKACPWIRGLNFDLPHVVSTSPEYENVQHVGGNMFDYVPKADAAF 284
           LVDVGGG GT L +I    P I+G+NFDL  V++ +P Y  V+HV G+MF  VPK DA F
Sbjct: 225 LVDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQAPFYPGVKHVSGDMFIEVPKGDAIF 284

Query: 285 FMWILHCWDDEECIKILKNCKEAIPKSRGKVIIVEIV--IDEKEKSKTSDMRLMLDMAMM 344
             WILH W DE+CIKILKNC +++P+ +GKVIIVE++  ++ K    + +  L +D+ M+
Sbjct: 285 MKWILHDWGDEDCIKILKNCWKSLPE-KGKVIIVEMITPMEPKPNDFSCNTVLGMDLLML 344

Query: 345 ARFKKGKERTADEWAYLLNEAGF 348
            +   GKER+  ++  L   +GF
Sbjct: 345 TQCSGGKERSLSQFENLAFASGF 362