MS022144 (gene) Bitter gourd (TR) v1

Overview
NameMS022144
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein of unknown function (DUF803)
Locationscaffold47: 1210912 .. 1213967 (+)
RNA-Seq ExpressionMS022144
SyntenyMS022144
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGATTTCGGAAAATACGAAGGGTCTGGTGTTGGCAATGGCGTCGAGTGCATTTATTGGATCGAGCTTTATCTTGAAGAAGAAAGGGCTTAAGCGTGCCGGTGCAACAGGGGCGAGAGCAGGTCTGTGATTACGATTCATGAATTATTTTTGGCTCGTTGGCTTTGCTTTTGCCTTCTGATTGAATTATTCTATCGATTGTATTTGTAGGTTGGATGCAATCTGATTAATTATGAATTTATTCCTTCTGCATTTGCTTTATGTCAGAACTTTGATTAATTTTGGGTTTTTTGACCTGGGATTGTTGTAAATGTTCTCATAATCGAATGTAAGAGATGAGGTCTTTGATTAGACTCGGATTTTGCAGTAGTTAGATCCGTATGGACTTTTCTTCTTGATCTTTCGAACGGAAGGAAGCTTTAAGGGTTGGGATTGGTTTCTCCAATAGGTCATTGATTCAGAACACTATCAACCATCTCTCGGATCTCAAACGTTTTGGGGGTGGAAGCTTTAAGACGGGGCATGCTTAACACATGCAAGTCGGACGGGAAGTGGTGTTTCAGTGGCGGACGTGTGAGTAACGCATAAGAACCTGCCTTTGGGAGGGGAACAACAGGAAACGACCGCTAATACCCCGTGTGCTGAGGAGAAAAAGTAGCTAGTTGGGTTGGGATTTGAGGTTCTTGATGAACTGGTATTTCTGAGCAAGCGTTAAGCTGTAAATAGAGTTAAAATCCTCTTAATGAACCAAAAAATTAAAGATCTTAAGCTGTATCATTGATTGATTTATTTTTTCACTTCTCTGTCATTGCTTCTAGTAGACGTTTAAATCAAGAGTCTCCCAGGCAATGTTTCATGCTCTCTAACTGGTTGATGTTTCTAATCTTTTTCTTTAAACAGGTGTTGGCGGTTATACGTACCTGTTAGAACCACTTTGGTGGGCTGGCATGGTTACAAGTATGTATAATAATACTATTATGTCTGATCATTATTGTCAAAACTCAAAAGTATGATCTTGATTCTGAGTTTTCTTCTTGGTAACTAGTGATTATTGGTGAGATTGCAAATTTTGTTGCATACATTTATGCCCCAGCAGTTCTAGTCACACCCCTCGGCGCACTGAGTATTATTATCAGGTAAGTTCCTTACTTTTGAGATTCTTGTATCATTTTTGTCTAGTTTAAGAATTGGTCAACTAGTTCATGGGGAACCTCACATTGTTTCTGGATCTATGCAGTGCTGTTTTAGCCCACTTATTGTTGAAGGAGAGGCTACAGAAAATGGGTGTTGTAGGGTGTTTATCCTGTATTGTAGGATCAGTTATAATAGTTATCCACGCACCTCAGGAACGTACTCCTGATTCCGTTCAAGAAATTTGGGATTTAGCAACCCAACCAGGTTAGAGCTTGTTCTTATAACTTTCATTTAATGAAATGTTATCGAATTTCGTACTTATTTCTTTTTTCTTTTTAATCCTTGGACAGCTTTTCTAGTCTACGTTGCTGCTACAATTTCATTAGTATTGGCTCTTATGTTGTATTTCGAACCTCGCTACGGGCATGTAAACATACTAGTCTACTTGGGAATCTGTTCTTTGATGGGCTCATTGACGGTAACTTTGACATTTCTGATACATCAATAGATGTATATATATATTATTTGAAATTTGAATCAAAATTTTCTAAACGTTTGTGATTTGTTCAGGTTATGAGTATTAAAGCTATTGGAATTGCGATAAGGCTTACACTTGAAGGGGTAAGCCAGGTAGCTTATCCTCAGACTTGGCTTTTTCTCACAGTTGCAGTAGTTTGCGTCGTCACACAATTAAATTACTTGAATAAGGTTGGTGTCATGTCAATGGTGGGGACAGTTATTTCTTGTTTCTTCGTGGTGACCGTTCTAGTGTTGGGGTGAGAACCGGTGGCTGCTCACCCTTCGAATTGAATGGTCAGCACGAAGGTTCAAGACTTAGCCGCGCCTTAGCAAAGCTAGAGCTTAACCAAATGCTTGGTACGGTCTTTTTTTTGTTTCAGGCATTGGATACATTTAACGCAGCACTCGTATCTCCAGTATATTATGCTATGTTCACGACATTGACTATTGTTGCTAGTGCTATCATGTTTAAGGTAAATTACTTGACCCCCTATAGATTCTTTGATCTTGTGTATTATATCCTATCCATACAAGTGAAACAAATGAAACGTCTAAAAAAAACTTGAATGTAGCTTGATTAAGCGGGTTTATGCTACAGGACTGGTTGGGCCAGGACACGAGCACAATAGTATCTGAATTATGTGGATTCATAACTGTCCTCTCTGGAACTATTATACTTCATTCAACCAGAGAACAGCCGCCAGTGTCCACACAAGGTATGACATCTAAACATCTCTTTTACATTTTCATGTTTATGATCAATTGAGAAATCCTAGTATGGTTTGATATCCACTCTTTTATTGCGGAATCCCGTACTACGAACGAATTGGTTGTGTATGTATGCTCATATAAACTGCATAGCGTTAAGAAGTGTAGTTATGGTTATGACATCTATCTAGCGTATCTGAATACTGCGATTGGTTCTTCTCTTGTATATGGTATCGTTTGGCAAAATATGGTCCTACACCCACCCCTCCCATAACAAAAAGGGGTCACATAAGAAAAACATAACTTTAATTTCTATCGGACATGGGATGATCTAGTGGTCAATAAGGGCCAATGTGAATAGCAAAGAGCTTAGAAGGAATGGGTTCAAACCATGGTGGCTACGAGTTTCCTTGTCAACCAAATGTGGTAGGGTCAAGCGGTTGAACCGTGATTAGTTGAGGTGCACACGAGTTGGCCCGGATACATGGATATCAGGGGAAAGAAAAATCAACTTCATTTAGTTGTTTGTTTAATGTGGCAGTACTGCAAATTGGTAGTAGAACTTGCACAATGCTAGAGACATCATGAGTTATACTTTCCTTTGCAGGATCTGTGGCATGGTATATTGGGGATTCAATGAAAGGTATTGAAGAACATTTGATCACCATAAGCAATTCACATTACACTGAAGAA

mRNA sequence

ATGAGGATTTCGGAAAATACGAAGGGTCTGGTGTTGGCAATGGCGTCGAGTGCATTTATTGGATCGAGCTTTATCTTGAAGAAGAAAGGGCTTAAGCGTGCCGGTGCAACAGGGGCGAGAGCAGGTGTTGGCGGTTATACGTACCTGTTAGAACCACTTTGGTGGGCTGGCATGGTTACAATGATTATTGGTGAGATTGCAAATTTTGTTGCATACATTTATGCCCCAGCAGTTCTAGTCACACCCCTCGGCGCACTGAGTATTATTATCAGTGCTGTTTTAGCCCACTTATTGTTGAAGGAGAGGCTACAGAAAATGGGTGTTGTAGGGTGTTTATCCTGTATTGTAGGATCAGTTATAATAGTTATCCACGCACCTCAGGAACGTACTCCTGATTCCGTTCAAGAAATTTGGGATTTAGCAACCCAACCAGCTTTTCTAGTCTACGTTGCTGCTACAATTTCATTAGTATTGGCTCTTATGTTGTATTTCGAACCTCGCTACGGGCATGTAAACATACTAGTCTACTTGGGAATCTGTTCTTTGATGGGCTCATTGACGGTTATGAGTATTAAAGCTATTGGAATTGCGATAAGGCTTACACTTGAAGGGGTAAGCCAGGTAGCTTATCCTCAGACTTGGCTTTTTCTCACAGTTGCAGTAGTTTGCGTCGTCACACAATTAAATTACTTGAATAAGGCATTGGATACATTTAACGCAGCACTCGTATCTCCAGTATATTATGCTATGTTCACGACATTGACTATTGTTGCTAGTGCTATCATGTTTAAGGACTGGTTGGGCCAGGACACGAGCACAATAGTATCTGAATTATGTGGATTCATAACTGTCCTCTCTGGAACTATTATACTTCATTCAACCAGAGAACAGCCGCCAGTGTCCACACAAGGATCTGTGGCATGGTATATTGGGGATTCAATGAAAGGTATTGAAGAACATTTGATCACCATAAGCAATTCACATTACACTGAAGAA

Coding sequence (CDS)

ATGAGGATTTCGGAAAATACGAAGGGTCTGGTGTTGGCAATGGCGTCGAGTGCATTTATTGGATCGAGCTTTATCTTGAAGAAGAAAGGGCTTAAGCGTGCCGGTGCAACAGGGGCGAGAGCAGGTGTTGGCGGTTATACGTACCTGTTAGAACCACTTTGGTGGGCTGGCATGGTTACAATGATTATTGGTGAGATTGCAAATTTTGTTGCATACATTTATGCCCCAGCAGTTCTAGTCACACCCCTCGGCGCACTGAGTATTATTATCAGTGCTGTTTTAGCCCACTTATTGTTGAAGGAGAGGCTACAGAAAATGGGTGTTGTAGGGTGTTTATCCTGTATTGTAGGATCAGTTATAATAGTTATCCACGCACCTCAGGAACGTACTCCTGATTCCGTTCAAGAAATTTGGGATTTAGCAACCCAACCAGCTTTTCTAGTCTACGTTGCTGCTACAATTTCATTAGTATTGGCTCTTATGTTGTATTTCGAACCTCGCTACGGGCATGTAAACATACTAGTCTACTTGGGAATCTGTTCTTTGATGGGCTCATTGACGGTTATGAGTATTAAAGCTATTGGAATTGCGATAAGGCTTACACTTGAAGGGGTAAGCCAGGTAGCTTATCCTCAGACTTGGCTTTTTCTCACAGTTGCAGTAGTTTGCGTCGTCACACAATTAAATTACTTGAATAAGGCATTGGATACATTTAACGCAGCACTCGTATCTCCAGTATATTATGCTATGTTCACGACATTGACTATTGTTGCTAGTGCTATCATGTTTAAGGACTGGTTGGGCCAGGACACGAGCACAATAGTATCTGAATTATGTGGATTCATAACTGTCCTCTCTGGAACTATTATACTTCATTCAACCAGAGAACAGCCGCCAGTGTCCACACAAGGATCTGTGGCATGGTATATTGGGGATTCAATGAAAGGTATTGAAGAACATTTGATCACCATAAGCAATTCACATTACACTGAAGAA

Protein sequence

MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPVSTQGSVAWYIGDSMKGIEEHLITISNSHYTEE
Homology
BLAST of MS022144 vs. NCBI nr
Match: XP_022136343.1 (probable magnesium transporter NIPA6 isoform X1 [Momordica charantia])

HSP 1 Score: 625.5 bits (1612), Expect = 2.5e-175
Identity = 330/332 (99.40%), Postives = 330/332 (99.40%), Query Frame = 0

Query: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT
Sbjct: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVI 120
           MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAH LLKERLQKMGVVGCLSCIVGSVI
Sbjct: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180
           IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPV 300
           ALVSPVYYAMFTTLTIVASAIMFKDWLGQD STIVSELCGFITVLSGTIILHSTREQPPV
Sbjct: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDASTIVSELCGFITVLSGTIILHSTREQPPV 300

Query: 301 STQGSVAWYIGDSMKGIEEHLITISNSHYTEE 333
           STQGSVAWYIGDSMKGIEEHLITISNSHYTEE
Sbjct: 301 STQGSVAWYIGDSMKGIEEHLITISNSHYTEE 332

BLAST of MS022144 vs. NCBI nr
Match: XP_038886953.1 (probable magnesium transporter NIPA6 isoform X1 [Benincasa hispida])

HSP 1 Score: 599.0 bits (1543), Expect = 2.5e-167
Identity = 314/333 (94.29%), Postives = 325/333 (97.60%), Query Frame = 0

Query: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGM+T
Sbjct: 1   MTISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMIT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVI 120
           MIIGEIANFVAYIYAPAVLVTPLGALSII+SAVLAH LL+ERLQKMGVVGCLSCIVGSVI
Sbjct: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLRERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180
           IVIHAPQERTPDSV+EIWDLATQPAFLVY+AA  SLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQERTPDSVEEIWDLATQPAFLVYIAAIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAIRLTL+GVSQVAYPQTWLF+TVAVVCV+TQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLDGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPV 300
           ALVSPVYYAMFTTLTI+ASAIMFKDW GQ+ STIVSELCGFITVLSGTIILHSTREQ PV
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNASTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 STQGSVAWYI-GDSMKGIEEHLITISNSHYTEE 333
           S+QGSVAWYI GDSMKGIEEHLITISNSHYTEE
Sbjct: 301 SSQGSVAWYISGDSMKGIEEHLITISNSHYTEE 333

BLAST of MS022144 vs. NCBI nr
Match: XP_023554276.1 (probable magnesium transporter NIPA6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 597.0 bits (1538), Expect = 9.6e-167
Identity = 314/332 (94.58%), Postives = 322/332 (96.99%), Query Frame = 0

Query: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT
Sbjct: 1   MTISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVI 120
           MIIGEIANF+AYIYAPAVLVTPLGALSIIISAVLAH LLKERLQKMGV+GCLSCIVGSVI
Sbjct: 61  MIIGEIANFIAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGVLGCLSCIVGSVI 120

Query: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180
           IVIHAPQE TPDSVQEIWDLATQPAFLVY+A   SLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVQEIWDLATQPAFLVYIATIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLF+TVAVVCV+TQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPV 300
           ALVSPVYYAMFTTLTI+ASAIMFKDW GQ+ +TIVSELCGFITVLSGTIILHSTREQ PV
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNANTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 STQGSVAWYI-GDSMKGIEEHLITISNSHYTE 332
           STQGSVAWYI GDSMKGIEEHLITISNSHYTE
Sbjct: 301 STQGSVAWYITGDSMKGIEEHLITISNSHYTE 332

BLAST of MS022144 vs. NCBI nr
Match: XP_008447976.1 (PREDICTED: probable magnesium transporter NIPA6 isoform X1 [Cucumis melo] >XP_008447977.1 PREDICTED: probable magnesium transporter NIPA6 isoform X1 [Cucumis melo])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 312/333 (93.69%), Postives = 323/333 (97.00%), Query Frame = 0

Query: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGL+LAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGM+T
Sbjct: 1   MTISENTKGLILAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMIT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVI 120
           MIIGEIANFVAYIYAPAVLVTPLGALSII+SAVLAH LLKERLQKMGVVGCLSCIVGSVI
Sbjct: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180
           IVIHAPQE TPDSV+EIWDLATQPAFLVY+AA  SLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVEEIWDLATQPAFLVYIAAIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAIRLTLEG+SQVAYPQTWLF+TVAVVCV+TQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGISQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPV 300
           ALVSPVYYAMFTTLTI+ASAIMFKDW GQ+ STIVSELCGFITVLSGTIILHSTREQ PV
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNASTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 STQGSVAWYI-GDSMKGIEEHLITISNSHYTEE 333
           S+QGSVAWYI GDSMK IEEHLITISNSHYTEE
Sbjct: 301 SSQGSVAWYISGDSMKSIEEHLITISNSHYTEE 333

BLAST of MS022144 vs. NCBI nr
Match: XP_022952466.1 (probable magnesium transporter NIPA6 isoform X1 [Cucurbita moschata] >KAG6572100.1 putative magnesium transporter NIPA6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 595.5 bits (1534), Expect = 2.8e-166
Identity = 313/332 (94.28%), Postives = 322/332 (96.99%), Query Frame = 0

Query: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT
Sbjct: 1   MTISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVI 120
           MIIGEIANF+AYIYAPAVLVTPLGALSIIISAVLAH LLKERLQKMGV+GCLSCIVGSVI
Sbjct: 61  MIIGEIANFIAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGVLGCLSCIVGSVI 120

Query: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180
           IVIHAPQE TPDSVQEIWDLATQPAFLVY+A   SLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVQEIWDLATQPAFLVYIATIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLF+TVAVVCV+TQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPV 300
           ALVSPVYYAMFTTLTI+ASAIMFKDW GQ+ +TIVSELCGFITVLSGTIILHSTREQ PV
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNANTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 STQGSVAWYI-GDSMKGIEEHLITISNSHYTE 332
           S+QGSVAWYI GDSMKGIEEHLITISNSHYTE
Sbjct: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTE 332

BLAST of MS022144 vs. ExPASy Swiss-Prot
Match: Q8GWX2 (Probable magnesium transporter NIPA6 OS=Arabidopsis thaliana OX=3702 GN=At2g21120 PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.5e-135
Identity = 251/328 (76.52%), Postives = 289/328 (88.11%), Query Frame = 0

Query: 4   SENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           ++N KGL+LA+ASS FIGSSFILKKKGLKRAGA G RAG GGYTYLLEPLWWAGMVTMI+
Sbjct: 3   TDNGKGLILAVASSVFIGSSFILKKKGLKRAGAIGTRAGYGGYTYLLEPLWWAGMVTMIV 62

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIVI 123
           GE ANFVAYIYAPAVLVTPLGALSIIISAVLAH LLKE+L+KMGV+GC+SCIVGSV+IVI
Sbjct: 63  GEAANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKEKLKKMGVLGCVSCIVGSVVIVI 122

Query: 124 HAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAP+E+TP+SV+EIW+LATQPAFL+YVA T+S+VLAL+L+FEP  G  NILVY+GICSLM
Sbjct: 123 HAPKEQTPNSVEEIWNLATQPAFLIYVAITMSIVLALILHFEPLCGQTNILVYIGICSLM 182

Query: 184 GSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAALV 243
           G+LTVMSIKAIGIAI+LT+EGVSQ+ YPQTWLF+ VAV CVVTQL YLNKALDTFNAA+V
Sbjct: 183 GALTVMSIKAIGIAIKLTMEGVSQIGYPQTWLFVMVAVTCVVTQLIYLNKALDTFNAAIV 242

Query: 244 SPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPVSTQ 303
           SPVYY MFTTLTIVASAIMFKDW GQD +++ SELCGFITVL+GT+ILH TRE+      
Sbjct: 243 SPVYYVMFTTLTIVASAIMFKDWSGQDAASVASELCGFITVLTGTMILHGTREEEQQQAS 302

Query: 304 GS-VAWYIGDSMKGI-EEHLITISNSHY 330
              V WY  DS K + EEHL+++ +  Y
Sbjct: 303 SEHVRWY--DSRKSMNEEHLVSLYSPEY 328

BLAST of MS022144 vs. ExPASy Swiss-Prot
Match: Q8GYS1 (Probable magnesium transporter NIPA7 OS=Arabidopsis thaliana OX=3702 GN=At4g38730 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.1e-125
Identity = 236/328 (71.95%), Postives = 276/328 (84.15%), Query Frame = 0

Query: 3   ISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMI 62
           +S+N  GLVLA++SS FIGSSFILKKKGLKRA A G RAG GGYTYLLEPLWW G+VTM 
Sbjct: 2   VSDNEMGLVLAVSSSVFIGSSFILKKKGLKRAAANGTRAGFGGYTYLLEPLWWVGLVTMT 61

Query: 63  IGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIV 122
            GEIANFVAY+YAPAVLVTPLGALSIIISAVLAH LL E+L+KMGV GC+ CIVGSV+IV
Sbjct: 62  FGEIANFVAYVYAPAVLVTPLGALSIIISAVLAHFLLDEKLRKMGVWGCVCCIVGSVMIV 121

Query: 123 IHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSL 182
           IHAPQE+TP+SV+EIW LA QPAFL+YVA ++S+VLAL+LY EP  G  NILVY+GICSL
Sbjct: 122 IHAPQEQTPNSVEEIWKLAMQPAFLIYVAISMSIVLALILYCEPLCGQTNILVYIGICSL 181

Query: 183 MGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAAL 242
           MGSLTVMSIKA+GIAI+LT EG++Q+ YP+TW F  VA +CVV Q+ YLNKALDTFNAA+
Sbjct: 182 MGSLTVMSIKAVGIAIKLTFEGINQIWYPETWFFAMVAAICVVMQMIYLNKALDTFNAAI 241

Query: 243 VSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPVST 302
           VSP+YY MFTTLTIVASAIMFKDW GQ+T +I SE+CGFITVL+GT+ILHSTRE+   S 
Sbjct: 242 VSPIYYVMFTTLTIVASAIMFKDWNGQNTDSIASEICGFITVLTGTVILHSTREEEQASP 301

Query: 303 QGSVAWYIGDSMKGI-EEHLITISNSHY 330
           +  + W   DS K   EEHL ++ +  Y
Sbjct: 302 R-RMRWQ--DSGKSFDEEHLTSLYSPEY 326

BLAST of MS022144 vs. ExPASy Swiss-Prot
Match: Q9LNK7 (Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana OX=3702 GN=At1g34470 PE=2 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 5.1e-110
Identity = 189/293 (64.51%), Postives = 250/293 (85.32%), Query Frame = 0

Query: 4   SENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGLVLA++SS FIG+SFI+KKKGLKRAGA+G RAG GGY+YLLEPLWW GM+TMI+
Sbjct: 17  SDNIKGLVLALSSSLFIGASFIVKKKGLKRAGASGLRAGSGGYSYLLEPLWWVGMITMIV 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIVI 123
           GEIANF AY +APA+LVTPLGALSIIISA LAH++L E+L   G++GC+ C+VGS+ IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVLCVVGSITIVL 136

Query: 124 HAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQE+  DSV ++W+LAT+PAFL+Y AA +   + L++ F P+YG  +++VY+G+CSL+
Sbjct: 137 HAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQSHVMVYIGVCSLV 196

Query: 184 GSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA++LT  G++Q+ YPQTW+F  + + CV+TQ+NYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFTLIVLTCVITQMNYLNKALDTFNTAVV 256

Query: 244 SPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTRE 297
           SP+YY MFT+LTI+AS IMFKDW  QD + IV+ELCGF+T+LSGT +LH T++
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTELCGFVTILSGTFLLHKTKD 309

BLAST of MS022144 vs. ExPASy Swiss-Prot
Match: Q94AH3 (Probable magnesium transporter NIPA4 OS=Arabidopsis thaliana OX=3702 GN=At1g71900 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 3.6e-108
Identity = 189/293 (64.51%), Postives = 245/293 (83.62%), Query Frame = 0

Query: 4   SENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGLVLA++SS FIG+SFI+KKKGLK+A +TG RAGVGGY+YL EPLWW GM TM++
Sbjct: 17  SDNIKGLVLALSSSLFIGASFIVKKKGLKKAASTGTRAGVGGYSYLYEPLWWIGMTTMLL 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIVI 123
           GEIANF AY +APA+LVTPLGA+SIIISAVLAH++L+E+L   G++GC  C+VGS  IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGAVSIIISAVLAHIILREKLHIFGILGCALCVVGSTTIVL 136

Query: 124 HAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQER  DSV E+W+LAT+PAF+ Y +  I   + L++ F P+YG  N++VY+GICSL+
Sbjct: 137 HAPQEREIDSVIEVWNLATEPAFMFYASLVIGAAVFLIIRFVPQYGQTNVMVYIGICSLV 196

Query: 184 GSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA++LT  G +Q+ YPQTW+F  V + CVVTQLNYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGTNQLFYPQTWIFTLVVLTCVVTQLNYLNKALDTFNTAIV 256

Query: 244 SPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTRE 297
           SP+YY MFT+LTI+AS IMFKDW  Q+ + IV+E+CGF+T+LSGT +LH T++
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQNGTQIVTEICGFVTILSGTFLLHRTKD 309

BLAST of MS022144 vs. ExPASy Swiss-Prot
Match: F4JKQ7 (Probable magnesium transporter NIPA5 OS=Arabidopsis thaliana OX=3702 GN=At4g09640 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 3.6e-108
Identity = 189/304 (62.17%), Postives = 251/304 (82.57%), Query Frame = 0

Query: 4   SENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGLVLA++SS FIG+SFI+KKKGLK+AGA+G RAG GGY+YLLEPLWW GM+TMI+
Sbjct: 17  SDNVKGLVLALSSSIFIGASFIVKKKGLKKAGASGLRAGSGGYSYLLEPLWWIGMITMIV 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIVI 123
           GEIANF AY +APA+LVTPLGALSIIISA LAH++L+E+L   G++GC  CIVGSV IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGALSIIISASLAHIILQEKLHTFGILGCALCIVGSVTIVL 136

Query: 124 HAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQE+   SV E+W+LAT+PAFL Y AA +   + L++ F P YG  +++VY+G+CSL+
Sbjct: 137 HAPQEQDIVSVLEVWNLATEPAFLFYAAAVVGAAIVLIVQFIPLYGQSHVMVYIGVCSLI 196

Query: 184 GSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA++LT  G +Q+ YPQTW+F  + + CV+TQ+NYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGTNQLGYPQTWVFTVIVLFCVITQMNYLNKALDTFNTAVV 256

Query: 244 SPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPVSTQ 303
           SP+YY MFT+LTI+AS IMFKDW  Q  + I++ELCGF+T+LSGT +LH+T +     ++
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQSGTQIMTELCGFVTILSGTFLLHTTTDMVDGESK 316

Query: 304 GSVA 308
           G+++
Sbjct: 317 GNLS 320

BLAST of MS022144 vs. ExPASy TrEMBL
Match: A0A6J1C7D2 (Probable magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111008053 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 330/332 (99.40%), Postives = 330/332 (99.40%), Query Frame = 0

Query: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT
Sbjct: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVI 120
           MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAH LLKERLQKMGVVGCLSCIVGSVI
Sbjct: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180
           IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPV 300
           ALVSPVYYAMFTTLTIVASAIMFKDWLGQD STIVSELCGFITVLSGTIILHSTREQPPV
Sbjct: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDASTIVSELCGFITVLSGTIILHSTREQPPV 300

Query: 301 STQGSVAWYIGDSMKGIEEHLITISNSHYTEE 333
           STQGSVAWYIGDSMKGIEEHLITISNSHYTEE
Sbjct: 301 STQGSVAWYIGDSMKGIEEHLITISNSHYTEE 332

BLAST of MS022144 vs. ExPASy TrEMBL
Match: A0A1S3BJK8 (Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103490304 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 8.0e-167
Identity = 312/333 (93.69%), Postives = 323/333 (97.00%), Query Frame = 0

Query: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGL+LAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGM+T
Sbjct: 1   MTISENTKGLILAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMIT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVI 120
           MIIGEIANFVAYIYAPAVLVTPLGALSII+SAVLAH LLKERLQKMGVVGCLSCIVGSVI
Sbjct: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180
           IVIHAPQE TPDSV+EIWDLATQPAFLVY+AA  SLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVEEIWDLATQPAFLVYIAAIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAIRLTLEG+SQVAYPQTWLF+TVAVVCV+TQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGISQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPV 300
           ALVSPVYYAMFTTLTI+ASAIMFKDW GQ+ STIVSELCGFITVLSGTIILHSTREQ PV
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNASTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 STQGSVAWYI-GDSMKGIEEHLITISNSHYTEE 333
           S+QGSVAWYI GDSMK IEEHLITISNSHYTEE
Sbjct: 301 SSQGSVAWYISGDSMKSIEEHLITISNSHYTEE 333

BLAST of MS022144 vs. ExPASy TrEMBL
Match: A0A6J1GKB0 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111455145 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.4e-166
Identity = 313/332 (94.28%), Postives = 322/332 (96.99%), Query Frame = 0

Query: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT
Sbjct: 1   MTISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVI 120
           MIIGEIANF+AYIYAPAVLVTPLGALSIIISAVLAH LLKERLQKMGV+GCLSCIVGSVI
Sbjct: 61  MIIGEIANFIAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGVLGCLSCIVGSVI 120

Query: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180
           IVIHAPQE TPDSVQEIWDLATQPAFLVY+A   SLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVQEIWDLATQPAFLVYIATIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLF+TVAVVCV+TQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPV 300
           ALVSPVYYAMFTTLTI+ASAIMFKDW GQ+ +TIVSELCGFITVLSGTIILHSTREQ PV
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNANTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 STQGSVAWYI-GDSMKGIEEHLITISNSHYTE 332
           S+QGSVAWYI GDSMKGIEEHLITISNSHYTE
Sbjct: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTE 332

BLAST of MS022144 vs. ExPASy TrEMBL
Match: A0A6J1EK14 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111435194 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.4e-166
Identity = 313/333 (93.99%), Postives = 324/333 (97.30%), Query Frame = 0

Query: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGL+LAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMV 
Sbjct: 1   MTISENTKGLILAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVI 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVI 120
           MIIGE+ANFVAYIYAPAVLVTPLGALSII+SAVLAH LLKERLQKMGVVGCLSCIVGSVI
Sbjct: 61  MIIGEVANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180
           IVIHAPQERTPDSV+EIW+LATQPAFLVY+AAT SLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQERTPDSVEEIWNLATQPAFLVYLAATASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLF+TVAVVCVVTQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFVTVAVVCVVTQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPV 300
           ALVSPVYYAMFTTLTI+ASAIMFKDW GQ+ STIVSELCGFITVLSGT+ILHSTREQ PV
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNASTIVSELCGFITVLSGTMILHSTREQQPV 300

Query: 301 STQGSVAWYI-GDSMKGIEEHLITISNSHYTEE 333
            +QGSVAWYI GDSMKGIEEHLITISNSHYTEE
Sbjct: 301 PSQGSVAWYISGDSMKGIEEHLITISNSHYTEE 333

BLAST of MS022144 vs. ExPASy TrEMBL
Match: A0A6J1JLB2 (Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111486241 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.0e-166
Identity = 312/333 (93.69%), Postives = 323/333 (97.00%), Query Frame = 0

Query: 1   MRISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGL+LAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMV 
Sbjct: 1   MSISENTKGLILAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVI 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVI 120
           MIIGE+ANFVAYIYAPAVLVTPLGALSII+SAVLAH LLKERLQKMGVVGCLSCIVGSVI
Sbjct: 61  MIIGEVANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVIHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGIC 180
           IVIHAPQERTPDSV+EIW+LATQPAFLVY+AAT SLVL LMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQERTPDSVEEIWNLATQPAFLVYLAATASLVLVLMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLF+TVAVVCVVTQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFVTVAVVCVVTQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPV 300
           ALVSPVYYAMFTTLTI+ASAIMFKDW GQ+ STIVSELCGFITVLSGT+ILHSTREQ PV
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNVSTIVSELCGFITVLSGTMILHSTREQQPV 300

Query: 301 STQGSVAWYI-GDSMKGIEEHLITISNSHYTEE 333
            +QGSVAWYI GDSMKGIEEHLITISNSHYTEE
Sbjct: 301 PSQGSVAWYISGDSMKGIEEHLITISNSHYTEE 333

BLAST of MS022144 vs. TAIR 10
Match: AT2G21120.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 482.3 bits (1240), Expect = 3.2e-136
Identity = 251/328 (76.52%), Postives = 289/328 (88.11%), Query Frame = 0

Query: 4   SENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           ++N KGL+LA+ASS FIGSSFILKKKGLKRAGA G RAG GGYTYLLEPLWWAGMVTMI+
Sbjct: 3   TDNGKGLILAVASSVFIGSSFILKKKGLKRAGAIGTRAGYGGYTYLLEPLWWAGMVTMIV 62

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIVI 123
           GE ANFVAYIYAPAVLVTPLGALSIIISAVLAH LLKE+L+KMGV+GC+SCIVGSV+IVI
Sbjct: 63  GEAANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKEKLKKMGVLGCVSCIVGSVVIVI 122

Query: 124 HAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAP+E+TP+SV+EIW+LATQPAFL+YVA T+S+VLAL+L+FEP  G  NILVY+GICSLM
Sbjct: 123 HAPKEQTPNSVEEIWNLATQPAFLIYVAITMSIVLALILHFEPLCGQTNILVYIGICSLM 182

Query: 184 GSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAALV 243
           G+LTVMSIKAIGIAI+LT+EGVSQ+ YPQTWLF+ VAV CVVTQL YLNKALDTFNAA+V
Sbjct: 183 GALTVMSIKAIGIAIKLTMEGVSQIGYPQTWLFVMVAVTCVVTQLIYLNKALDTFNAAIV 242

Query: 244 SPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPVSTQ 303
           SPVYY MFTTLTIVASAIMFKDW GQD +++ SELCGFITVL+GT+ILH TRE+      
Sbjct: 243 SPVYYVMFTTLTIVASAIMFKDWSGQDAASVASELCGFITVLTGTMILHGTREEEQQQAS 302

Query: 304 GS-VAWYIGDSMKGI-EEHLITISNSHY 330
              V WY  DS K + EEHL+++ +  Y
Sbjct: 303 SEHVRWY--DSRKSMNEEHLVSLYSPEY 328

BLAST of MS022144 vs. TAIR 10
Match: AT4G38730.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 451.1 bits (1159), Expect = 8.0e-127
Identity = 236/328 (71.95%), Postives = 276/328 (84.15%), Query Frame = 0

Query: 3   ISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMI 62
           +S+N  GLVLA++SS FIGSSFILKKKGLKRA A G RAG GGYTYLLEPLWW G+VTM 
Sbjct: 2   VSDNEMGLVLAVSSSVFIGSSFILKKKGLKRAAANGTRAGFGGYTYLLEPLWWVGLVTMT 61

Query: 63  IGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIV 122
            GEIANFVAY+YAPAVLVTPLGALSIIISAVLAH LL E+L+KMGV GC+ CIVGSV+IV
Sbjct: 62  FGEIANFVAYVYAPAVLVTPLGALSIIISAVLAHFLLDEKLRKMGVWGCVCCIVGSVMIV 121

Query: 123 IHAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSL 182
           IHAPQE+TP+SV+EIW LA QPAFL+YVA ++S+VLAL+LY EP  G  NILVY+GICSL
Sbjct: 122 IHAPQEQTPNSVEEIWKLAMQPAFLIYVAISMSIVLALILYCEPLCGQTNILVYIGICSL 181

Query: 183 MGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAAL 242
           MGSLTVMSIKA+GIAI+LT EG++Q+ YP+TW F  VA +CVV Q+ YLNKALDTFNAA+
Sbjct: 182 MGSLTVMSIKAVGIAIKLTFEGINQIWYPETWFFAMVAAICVVMQMIYLNKALDTFNAAI 241

Query: 243 VSPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPVST 302
           VSP+YY MFTTLTIVASAIMFKDW GQ+T +I SE+CGFITVL+GT+ILHSTRE+   S 
Sbjct: 242 VSPIYYVMFTTLTIVASAIMFKDWNGQNTDSIASEICGFITVLTGTVILHSTREEEQASP 301

Query: 303 QGSVAWYIGDSMKGI-EEHLITISNSHY 330
           +  + W   DS K   EEHL ++ +  Y
Sbjct: 302 R-RMRWQ--DSGKSFDEEHLTSLYSPEY 326

BLAST of MS022144 vs. TAIR 10
Match: AT1G34470.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 399.1 bits (1024), Expect = 3.6e-111
Identity = 189/293 (64.51%), Postives = 250/293 (85.32%), Query Frame = 0

Query: 4   SENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGLVLA++SS FIG+SFI+KKKGLKRAGA+G RAG GGY+YLLEPLWW GM+TMI+
Sbjct: 17  SDNIKGLVLALSSSLFIGASFIVKKKGLKRAGASGLRAGSGGYSYLLEPLWWVGMITMIV 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIVI 123
           GEIANF AY +APA+LVTPLGALSIIISA LAH++L E+L   G++GC+ C+VGS+ IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVLCVVGSITIVL 136

Query: 124 HAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQE+  DSV ++W+LAT+PAFL+Y AA +   + L++ F P+YG  +++VY+G+CSL+
Sbjct: 137 HAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQSHVMVYIGVCSLV 196

Query: 184 GSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA++LT  G++Q+ YPQTW+F  + + CV+TQ+NYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFTLIVLTCVITQMNYLNKALDTFNTAVV 256

Query: 244 SPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTRE 297
           SP+YY MFT+LTI+AS IMFKDW  QD + IV+ELCGF+T+LSGT +LH T++
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTELCGFVTILSGTFLLHKTKD 309

BLAST of MS022144 vs. TAIR 10
Match: AT1G71900.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 392.9 bits (1008), Expect = 2.6e-109
Identity = 189/293 (64.51%), Postives = 245/293 (83.62%), Query Frame = 0

Query: 4   SENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGLVLA++SS FIG+SFI+KKKGLK+A +TG RAGVGGY+YL EPLWW GM TM++
Sbjct: 17  SDNIKGLVLALSSSLFIGASFIVKKKGLKKAASTGTRAGVGGYSYLYEPLWWIGMTTMLL 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIVI 123
           GEIANF AY +APA+LVTPLGA+SIIISAVLAH++L+E+L   G++GC  C+VGS  IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGAVSIIISAVLAHIILREKLHIFGILGCALCVVGSTTIVL 136

Query: 124 HAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQER  DSV E+W+LAT+PAF+ Y +  I   + L++ F P+YG  N++VY+GICSL+
Sbjct: 137 HAPQEREIDSVIEVWNLATEPAFMFYASLVIGAAVFLIIRFVPQYGQTNVMVYIGICSLV 196

Query: 184 GSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA++LT  G +Q+ YPQTW+F  V + CVVTQLNYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGTNQLFYPQTWIFTLVVLTCVVTQLNYLNKALDTFNTAIV 256

Query: 244 SPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTRE 297
           SP+YY MFT+LTI+AS IMFKDW  Q+ + IV+E+CGF+T+LSGT +LH T++
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQNGTQIVTEICGFVTILSGTFLLHRTKD 309

BLAST of MS022144 vs. TAIR 10
Match: AT4G09640.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 392.9 bits (1008), Expect = 2.6e-109
Identity = 189/304 (62.17%), Postives = 251/304 (82.57%), Query Frame = 0

Query: 4   SENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGLVLA++SS FIG+SFI+KKKGLK+AGA+G RAG GGY+YLLEPLWW GM+TMI+
Sbjct: 17  SDNVKGLVLALSSSIFIGASFIVKKKGLKKAGASGLRAGSGGYSYLLEPLWWIGMITMIV 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHLLLKERLQKMGVVGCLSCIVGSVIIVI 123
           GEIANF AY +APA+LVTPLGALSIIISA LAH++L+E+L   G++GC  CIVGSV IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGALSIIISASLAHIILQEKLHTFGILGCALCIVGSVTIVL 136

Query: 124 HAPQERTPDSVQEIWDLATQPAFLVYVAATISLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQE+   SV E+W+LAT+PAFL Y AA +   + L++ F P YG  +++VY+G+CSL+
Sbjct: 137 HAPQEQDIVSVLEVWNLATEPAFLFYAAAVVGAAIVLIVQFIPLYGQSHVMVYIGVCSLI 196

Query: 184 GSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFLTVAVVCVVTQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA++LT  G +Q+ YPQTW+F  + + CV+TQ+NYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGTNQLGYPQTWVFTVIVLFCVITQMNYLNKALDTFNTAVV 256

Query: 244 SPVYYAMFTTLTIVASAIMFKDWLGQDTSTIVSELCGFITVLSGTIILHSTREQPPVSTQ 303
           SP+YY MFT+LTI+AS IMFKDW  Q  + I++ELCGF+T+LSGT +LH+T +     ++
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQSGTQIMTELCGFVTILSGTFLLHTTTDMVDGESK 316

Query: 304 GSVA 308
           G+++
Sbjct: 317 GNLS 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136343.12.5e-17599.40probable magnesium transporter NIPA6 isoform X1 [Momordica charantia][more]
XP_038886953.12.5e-16794.29probable magnesium transporter NIPA6 isoform X1 [Benincasa hispida][more]
XP_023554276.19.6e-16794.58probable magnesium transporter NIPA6 [Cucurbita pepo subsp. pepo][more]
XP_008447976.11.6e-16693.69PREDICTED: probable magnesium transporter NIPA6 isoform X1 [Cucumis melo] >XP_00... [more]
XP_022952466.12.8e-16694.28probable magnesium transporter NIPA6 isoform X1 [Cucurbita moschata] >KAG6572100... [more]
Match NameE-valueIdentityDescription
Q8GWX24.5e-13576.52Probable magnesium transporter NIPA6 OS=Arabidopsis thaliana OX=3702 GN=At2g2112... [more]
Q8GYS11.1e-12571.95Probable magnesium transporter NIPA7 OS=Arabidopsis thaliana OX=3702 GN=At4g3873... [more]
Q9LNK75.1e-11064.51Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana OX=3702 GN=At1g3447... [more]
Q94AH33.6e-10864.51Probable magnesium transporter NIPA4 OS=Arabidopsis thaliana OX=3702 GN=At1g7190... [more]
F4JKQ73.6e-10862.17Probable magnesium transporter NIPA5 OS=Arabidopsis thaliana OX=3702 GN=At4g0964... [more]
Match NameE-valueIdentityDescription
A0A6J1C7D21.2e-17599.40Probable magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111008053 PE... [more]
A0A1S3BJK88.0e-16793.69Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103490304 PE=3 SV=1[more]
A0A6J1GKB01.4e-16694.28Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111455145 PE=... [more]
A0A6J1EK141.4e-16693.99Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111435194 PE=... [more]
A0A6J1JLB24.0e-16693.69Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111486241 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT2G21120.13.2e-13676.52Protein of unknown function (DUF803) [more]
AT4G38730.18.0e-12771.95Protein of unknown function (DUF803) [more]
AT1G34470.13.6e-11164.51Protein of unknown function (DUF803) [more]
AT1G71900.12.6e-10964.51Protein of unknown function (DUF803) [more]
AT4G09640.12.6e-10962.17Protein of unknown function (DUF803) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008521Magnesium transporter NIPAPFAMPF05653Mg_trans_NIPAcoord: 6..296
e-value: 1.3E-138
score: 461.1
IPR008521Magnesium transporter NIPAPANTHERPTHR12570UNCHARACTERIZEDcoord: 3..331
NoneNo IPR availablePANTHERPTHR12570:SF11MAGNESIUM TRANSPORTER NIPA6-RELATEDcoord: 3..331
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 52..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS022144.1MS022144.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015693 magnesium ion transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity