MS022093 (gene) Bitter gourd (TR) v1

Overview


Name	MS022093
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cysteine Protease
Location	scaffold47: 815096 .. 815203 (+)
RNA-Seq Expression	MS022093
Synteny	MS022093

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATCTTGCGGGTCCTGTACAACTGGTGCTCTAGAAGGTGCCAATTTTCTTGCAGAAGGCGAACTTGTTAGCATAAGCGAACAGCGGCCGGTAGATTGCGATCACGAG

mRNA sequence

GGATCTTGCGGGTCCTGTACAACTGGTGCTCTAGAAGGTGCCAATTTTCTTGCAGAAGGCGAACTTGTTAGCATAAGCGAACAGCGGCCGGTAGATTGCGATCACGAG

Coding sequence (CDS)

GGATCTTGCGGGTCCTGTACAACTGGTGCTCTAGAAGGTGCCAATTTTCTTGCAGAAGGCGAACTTGTTAGCATAAGCGAACAGCGGCCGGTAGATTGCGATCACGAG

Protein sequence

GSCGSCTTGALEGANFLAEGELVSISEQRPVDCDHE

Homology

[Close]

BLAST of MS022093 vs. NCBI nr
Match: PON77411.1 (Cyseine protease [Trema orientale])

HSP 1 Score: 63.9 bits (154), Expect = 3.2e-07
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGANFLA GELVS+SEQ+ VDCDHE
Sbjct: 156 GSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHE 195

[Close]

BLAST of MS022093 vs. NCBI nr
Match: XP_004148554.2 (cysteine protease RD19A [Cucumis sativus] >KGN43159.1 hypothetical protein Csa_020172 [Cucumis sativus])

HSP 1 Score: 63.9 bits (154), Expect = 3.2e-07
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGANFLA GELVS+SEQ+ VDCDHE
Sbjct: 163 GSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHE 202

[Close]

BLAST of MS022093 vs. NCBI nr
Match: PON80790.1 (Cysteine Protease [Parasponia andersonii])

HSP 1 Score: 63.9 bits (154), Expect = 3.2e-07
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGANFLA GELVS+SEQ+ VDCDHE
Sbjct: 155 GSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHE 194

[Close]

BLAST of MS022093 vs. NCBI nr
Match: VFQ72182.1 (unnamed protein product [Cuscuta campestris])

HSP 1 Score: 63.9 bits (154), Expect = 3.2e-07
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGANFLA GELVS+SEQ+ VDCDHE
Sbjct: 155 GSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHE 194

[Close]

BLAST of MS022093 vs. NCBI nr
Match: VFQ71874.1 (unnamed protein product [Cuscuta campestris])

HSP 1 Score: 63.9 bits (154), Expect = 3.2e-07
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGANFLA GELVS+SEQ+ VDCDHE
Sbjct: 156 GSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHE 195

[Close]

BLAST of MS022093 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 4.6e-09
Identity = 30/40 (75.00%), Postives = 33/40 (82.50%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC     TGALEGANFLA G+LVS+SEQ+ VDCDHE
Sbjct: 154 GSCGSCWSFSATGALEGANFLATGKLVSLSEQQLVDCDHE 193

[Close]

BLAST of MS022093 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 4.6e-09
Identity = 30/40 (75.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGA+FLA G+LVS+SEQ+ VDCDHE
Sbjct: 151 GSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQLVDCDHE 190

[Close]

BLAST of MS022093 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.9e-08
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDH 36
           GSCGSC    TTGALEGA++LA G+LVS+SEQ+ VDCDH
Sbjct: 151 GSCGSCWAFSTTGALEGAHYLATGKLVSLSEQQLVDCDH 189

[Close]

BLAST of MS022093 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 9.6e-07
Identity = 27/40 (67.50%), Postives = 30/40 (75.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           G CGSC      GALEGA+FLA  ELVS+SEQ+ VDCDHE
Sbjct: 159 GMCGSCWSFSAIGALEGAHFLATKELVSLSEQQLVDCDHE 198

[Close]

BLAST of MS022093 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 3.7e-06
Identity = 25/40 (62.50%), Postives = 31/40 (77.50%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC     +GALEGA++LA G+L  +SEQ+ VDCDHE
Sbjct: 156 GSCGSCWSFSASGALEGAHYLATGKLEVLSEQQFVDCDHE 195

[Close]

BLAST of MS022093 vs. ExPASy TrEMBL
Match: A0A2P5E5L4 (Cysteine Protease OS=Parasponia andersonii OX=3476 GN=PanCP23 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-07
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGANFLA GELVS+SEQ+ VDCDHE
Sbjct: 155 GSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHE 194

[Close]

BLAST of MS022093 vs. ExPASy TrEMBL
Match: A0A0A0K2B5 (Papain-like cysteine proteinase isoform I OS=Cucumis sativus OX=3659 GN=Csa_7G004120 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-07
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGANFLA GELVS+SEQ+ VDCDHE
Sbjct: 163 GSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHE 202

[Close]

BLAST of MS022093 vs. ExPASy TrEMBL
Match: A0A7J6FFW6 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_021386 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-07
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGANFLA GELVS+SEQ+ VDCDHE
Sbjct: 152 GSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHE 191

[Close]

BLAST of MS022093 vs. ExPASy TrEMBL
Match: A0A7J6FXJ0 (Uncharacterized protein (Fragment) OS=Cannabis sativa OX=3483 GN=F8388_024125 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-07
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGANFLA GELVS+SEQ+ VDCDHE
Sbjct: 171 GSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHE 210

[Close]

BLAST of MS022093 vs. ExPASy TrEMBL
Match: A0A7J6GKM8 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_023991 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-07
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGANFLA GELVS+SEQ+ VDCDHE
Sbjct: 152 GSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHE 191

[Close]

BLAST of MS022093 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 60.5 bits (145), Expect = 3.3e-10
Identity = 30/40 (75.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC    TTGALEGA+FLA G+LVS+SEQ+ VDCDHE
Sbjct: 151 GSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQLVDCDHE 190

[Close]

BLAST of MS022093 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 60.5 bits (145), Expect = 3.3e-10
Identity = 30/40 (75.00%), Postives = 33/40 (82.50%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           GSCGSC     TGALEGANFLA G+LVS+SEQ+ VDCDHE
Sbjct: 154 GSCGSCWSFSATGALEGANFLATGKLVSLSEQQLVDCDHE 193

[Close]

BLAST of MS022093 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 52.8 bits (125), Expect = 6.8e-08
Identity = 27/40 (67.50%), Postives = 30/40 (75.00%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCDHE 37
           G CGSC      GALEGA+FLA  ELVS+SEQ+ VDCDHE
Sbjct: 159 GMCGSCWSFSAIGALEGAHFLATKELVSLSEQQLVDCDHE 198

[Close]

BLAST of MS022093 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 48.9 bits (115), Expect = 9.9e-07
Identity = 23/38 (60.53%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCD 35
           G+CGSC    TTGA EGA+F++ G+L+S+SEQ+ VDCD
Sbjct: 156 GACGSCWAFSTTGAAEGAHFVSTGKLLSLSEQQLVDCD 193

[Close]

BLAST of MS022093 vs. TAIR 10
Match: AT3G43960.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 8.4e-06
Identity = 22/38 (57.89%), Postives = 26/38 (68.42%), Query Frame = 0


Query: 1   GSCGSC----TTGALEGANFLAEGELVSISEQRPVDCD 35
           G CGSC     TGA+EG N +  GELVS+SEQ  +DCD
Sbjct: 147 GECGSCWAFAATGAVEGINQITTGELVSLSEQELIDCD 184

The following BLAST results are available for this feature:

BLAST of MS022093 vs. NCBI nr


Match Name	E-value	Identity	Description
PON77411.1	3.2e-07	80.00	Cyseine protease [Trema orientale]	[more]
XP_004148554.2	3.2e-07	80.00	cysteine protease RD19A [Cucumis sativus] >KGN43159.1 hypothetical protein Csa_0...	[more]
PON80790.1	3.2e-07	80.00	Cysteine Protease [Parasponia andersonii]	[more]
VFQ72182.1	3.2e-07	80.00	unnamed protein product [Cuscuta campestris]	[more]
VFQ71874.1	3.2e-07	80.00	unnamed protein product [Cuscuta campestris]	[more]

BLAST of MS022093 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43296	4.6e-09	75.00	Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1	[more]
P43295	4.6e-09	75.00	Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S...	[more]
P25804	3.9e-08	71.79	Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
Q9SUL1	9.6e-07	67.50	Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S...	[more]
Q10716	3.7e-06	62.50	Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1	[more]

BLAST of MS022093 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2P5E5L4	1.5e-07	80.00	Cysteine Protease OS=Parasponia andersonii OX=3476 GN=PanCP23 PE=3 SV=1	[more]
A0A0A0K2B5	1.5e-07	80.00	Papain-like cysteine proteinase isoform I OS=Cucumis sativus OX=3659 GN=Csa_7G00...	[more]
A0A7J6FFW6	1.5e-07	80.00	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_021386 PE=3 SV=1	[more]
A0A7J6FXJ0	1.5e-07	80.00	Uncharacterized protein (Fragment) OS=Cannabis sativa OX=3483 GN=F8388_024125 PE...	[more]
A0A7J6GKM8	1.5e-07	80.00	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_023991 PE=3 SV=1	[more]

BLAST of MS022093 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21430.1	3.3e-10	75.00	Papain family cysteine protease	[more]
AT4G39090.1	3.3e-10	75.00	Papain family cysteine protease	[more]
AT4G16190.1	6.8e-08	67.50	Papain family cysteine protease	[more]
AT3G54940.2	9.9e-07	60.53	Papain family cysteine protease	[more]
AT3G43960.1	8.4e-06	57.89	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 1..35 e-value: 5.1E-6 score: 26.7
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 1..36 e-value: 8.9E-7 score: 30.7
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 1..36

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS022093.1	MS022093.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008234	cysteine-type peptidase activity