MS022004 (gene) Bitter gourd (TR) v1

BLAST of MS022004 vs. NCBI nr
Match: XP_022149825.1 (stromal processing peptidase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 2451.8 bits (6353), Expect = 0.0e+00
Identity = 1257/1285 (97.82%), Postives = 1257/1285 (97.82%), Query Frame = 0

Query: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRSCTPLARFNVDSRFVVSLRRH 60
            MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRSCTPLARFNVDSRFVVSLRRH
Sbjct: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRSCTPLARFNVDSRFVVSLRRH 60

Query: 61   SHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRFTPRF 120
            SHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRFTPRF
Sbjct: 61   SHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRFTPRF 120

Query: 121  IFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPEFGR 180
            IFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAW DGILEKQDLDISYPEFGR
Sbjct: 121  IFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWSDGILEKQDLDISYPEFGR 180

Query: 181  AELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGI 240
            AELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGI
Sbjct: 181  AELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGI 240

Query: 241  AHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDALN 300
            AHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDALN
Sbjct: 241  AHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDALN 300

Query: 301  EIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQI 360
            EIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQI
Sbjct: 301  EIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQI 360

Query: 361  KKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPSA 420
            KKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPSA
Sbjct: 361  KKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPSA 420

Query: 421  FGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVDTNP 480
            FGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERH IRPPVKHNWSLPGSNV TNP
Sbjct: 421  FGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHAIRPPVKHNWSLPGSNVYTNP 480

Query: 481  PQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPFT 540
            PQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPFT
Sbjct: 481  PQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPFT 540

Query: 541  SIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDALL 600
            SIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDALL
Sbjct: 541  SIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDALL 600

Query: 601  KDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGAE 660
            KDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGAE
Sbjct: 601  KDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGAE 660

Query: 661  VLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAEP 720
            VLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAEP
Sbjct: 661  VLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAEP 720

Query: 721  ELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKIS 780
            ELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKIS
Sbjct: 721  ELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKIS 780

Query: 781  KSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINC 840
            KSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINC
Sbjct: 781  KSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINC 840

Query: 841  SLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQLYM 900
            SLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE         HSVWLEDAFDRAKQLYM
Sbjct: 841  SLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE---------HSVWLEDAFDRAKQLYM 900

Query: 901  SYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNMEV 960
            SYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNMEV
Sbjct: 901  SYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNMEV 960

Query: 961  SLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTDERA 1020
            SLVGDFSEEEVES ILDYLGTVTATTNSERAPPSVPIVFR SPSELQFQQVFLKDTDERA
Sbjct: 961  SLVGDFSEEEVESSILDYLGTVTATTNSERAPPSVPIVFRTSPSELQFQQVFLKDTDERA 1020

Query: 1021 CAYISGPAPNRWGVTVDGLELLETVSHISRTD----VNDAFDNDIEKGLQRKLRSHPLFF 1080
            CAYISGPAPNRWGVTVDGLELLETVSHISRTD    VNDAFDNDIEKGLQRKLRSHPLFF
Sbjct: 1021 CAYISGPAPNRWGVTVDGLELLETVSHISRTDATTPVNDAFDNDIEKGLQRKLRSHPLFF 1080

Query: 1081 GITMGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTS 1140
            GITMGLLAEIINSR          LFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTS
Sbjct: 1081 GITMGLLAEIINSR----------LFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTS 1140

Query: 1141 TPGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQAS 1200
            TPGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQAS
Sbjct: 1141 TPGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQAS 1200

Query: 1201 SVPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEE 1260
            SVPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEE
Sbjct: 1201 SVPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEE 1260

Query: 1261 EGSDQDFQGVVPTGRGLSTMTRPTT 1282
            EGSDQDFQGVVPTGRGLSTMTRPTT
Sbjct: 1261 EGSDQDFQGVVPTGRGLSTMTRPTT 1266

BLAST of MS022004 vs. NCBI nr
Match: XP_038889835.1 (stromal processing peptidase, chloroplastic [Benincasa hispida])

HSP 1 Score: 2302.3 bits (5965), Expect = 0.0e+00
Identity = 1180/1282 (92.04%), Postives = 1210/1282 (94.38%), Query Frame = 0

Query: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRS-CTPLARFNVDSRFVVSLRR 60
            MAVA+SSTVSNLTQRRPLLSLKD   PNRR+NS++LP RS C  L RF+V+ RF V L R
Sbjct: 1    MAVATSSTVSNLTQRRPLLSLKDPSTPNRRVNSVQLPSRSICAHLPRFDVEPRFFVPLTR 60

Query: 61   HSHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRFTPR 120
            +S DDGIGRYK RRNKDN RRP A K+ ERGNET GT NCISC LNQKR   SIKR TPR
Sbjct: 61   YSRDDGIGRYKIRRNKDNTRRPCAYKMGERGNETLGTTNCISCFLNQKRRCPSIKRPTPR 120

Query: 121  FIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPEFG 180
            FI DKSA +LSKNE DD+VVKH  IVC TVGPDEPHAA TAWPDGILEKQDLD SYPEFG
Sbjct: 121  FILDKSAFQLSKNERDDRVVKHTRIVCGTVGPDEPHAATTAWPDGILEKQDLDTSYPEFG 180

Query: 181  RAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG 240
            RAELEAFLSSELPSHPKLYRGQLKNGL+YLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG
Sbjct: 181  RAELEAFLSSELPSHPKLYRGQLKNGLKYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG 240

Query: 241  IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDAL 300
            IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDAL
Sbjct: 241  IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDAL 300

Query: 301  NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQ 360
            NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLS+RFPIGLEEQ
Sbjct: 301  NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQ 360

Query: 361  IKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPS 420
            IKKWDADKIRKFHERWYFPANATLYIVGDIDNI+K VNQIEAVF ETG+ENEAVSTPNPS
Sbjct: 361  IKKWDADKIRKFHERWYFPANATLYIVGDIDNISKAVNQIEAVFGETGLENEAVSTPNPS 420

Query: 421  AFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVDTN 480
            AFGAMASFLVPKISVGL GSLSNERSNSVDQSKIIKKERH IRPPVKHNWSLPGSNVD N
Sbjct: 421  AFGAMASFLVPKISVGLGGSLSNERSNSVDQSKIIKKERHAIRPPVKHNWSLPGSNVDAN 480

Query: 481  PPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF 540
            PPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF
Sbjct: 481  PPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF 540

Query: 541  TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL 600
            TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL
Sbjct: 541  TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL 600

Query: 601  LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA 660
            LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA
Sbjct: 601  LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA 660

Query: 661  EVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAE 720
            EVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEI TAIEAGL EPIEAE
Sbjct: 661  EVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEITTAIEAGLGEPIEAE 720

Query: 721  PELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKI 780
            PELEVPKELISSSQI+ELRMQ +PSF+PLNPET+VTKFHD ETGITQCRLSNGIPVNYKI
Sbjct: 721  PELEVPKELISSSQISELRMQHQPSFVPLNPETNVTKFHDNETGITQCRLSNGIPVNYKI 780

Query: 781  SKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLIN 840
            SKSENKAGVMRLIVGGGRAAESPD+QGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLIN
Sbjct: 781  SKSENKAGVMRLIVGGGRAAESPDAQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLIN 840

Query: 841  CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQLY 900
            CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE         HSVWLEDAFDRAKQLY
Sbjct: 841  CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE---------HSVWLEDAFDRAKQLY 900

Query: 901  MSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNME 960
            MSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVK+AVMNQFVGNNME
Sbjct: 901  MSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKDAVMNQFVGNNME 960

Query: 961  VSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTDER 1020
            VSLVGDFSEEE+ESCILDYLGTVTATT SE AP SVPIVFRPSPSELQFQQVFLKDTDER
Sbjct: 961  VSLVGDFSEEEIESCILDYLGTVTATTTSETAPASVPIVFRPSPSELQFQQVFLKDTDER 1020

Query: 1021 ACAYISGPAPNRWGVTVDGLELLETVSHISRTDVNDAFDNDIEKGLQRKLRSHPLFFGIT 1080
            ACAYISGPAPNRWGVT +GLELLE+VS ISRTD +D  DNDI+KGLQRKLRSHPLFFGIT
Sbjct: 1021 ACAYISGPAPNRWGVTFEGLELLESVSQISRTDESDKSDNDIKKGLQRKLRSHPLFFGIT 1080

Query: 1081 MGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTSTPG 1140
            MGLLAEIINSR          LFTSVRDSLGLTYDVSFE+SLFDRLKLGWYVISVTSTP 
Sbjct: 1081 MGLLAEIINSR----------LFTSVRDSLGLTYDVSFELSLFDRLKLGWYVISVTSTPA 1140

Query: 1141 KVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP 1200
            KVYKAVDACK+VLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP
Sbjct: 1141 KVYKAVDACKSVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP 1200

Query: 1201 RKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEEEGS 1260
            RKDLSCIKDLTSLYEAATIDD+YIAYDQLKVDADSLYTCIGIAGAQAGEE+I SFEEEGS
Sbjct: 1201 RKDLSCIKDLTSLYEAATIDDVYIAYDQLKVDADSLYTCIGIAGAQAGEESIVSFEEEGS 1260

Query: 1261 DQDFQGVVPTGRGLSTMTRPTT 1282
            DQDFQGVV +GRGLSTMTRPTT
Sbjct: 1261 DQDFQGVVSSGRGLSTMTRPTT 1263

BLAST of MS022004 vs. NCBI nr
Match: XP_023537547.1 (stromal processing peptidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2297.3 bits (5952), Expect = 0.0e+00
Identity = 1175/1282 (91.65%), Postives = 1213/1282 (94.62%), Query Frame = 0

Query: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRS-CTPLARFNVDSRFVVSLRR 60
            MAVA+SSTVSNLTQRRPLLSL+D G PNRR+NS++LP RS C+ LARF+V+SRFVV LRR
Sbjct: 1    MAVATSSTVSNLTQRRPLLSLRDPGTPNRRVNSVQLPSRSICSNLARFDVESRFVVPLRR 60

Query: 61   HSHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRFTPR 120
            +SHDDG GRYKFRRNKDNARRP A KI ERG+ TS T NCISC LNQKR   SIKRFTPR
Sbjct: 61   YSHDDGTGRYKFRRNKDNARRPHAYKIGERGSGTSETTNCISCFLNQKRRGPSIKRFTPR 120

Query: 121  FIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPEFG 180
            FIFDKSA +LSKNE D KVVKHA IVC TVGPDEPHAA T WPDGILEKQDLD SYPEFG
Sbjct: 121  FIFDKSAFQLSKNERDVKVVKHARIVCGTVGPDEPHAATTDWPDGILEKQDLDASYPEFG 180

Query: 181  RAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG 240
            RAELEAFLSSELPSHPKL+RGQLKNGL+YLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG
Sbjct: 181  RAELEAFLSSELPSHPKLHRGQLKNGLKYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG 240

Query: 241  IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDAL 300
            IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKD+DGDLLPSVLDAL
Sbjct: 241  IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDTDGDLLPSVLDAL 300

Query: 301  NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQ 360
            NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQ
Sbjct: 301  NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQ 360

Query: 361  IKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPS 420
            IKKWDADKIRKFHERWYFPANATLYIVGDIDNI+KTVNQIEAVF +TG+E EAVSTPNPS
Sbjct: 361  IKKWDADKIRKFHERWYFPANATLYIVGDIDNISKTVNQIEAVFDDTGLEKEAVSTPNPS 420

Query: 421  AFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVDTN 480
            AFGAMASFLVPKISVGL GSLSNERSNSVDQ KIIKKERH IRPPVKHNWSLPGSNVD N
Sbjct: 421  AFGAMASFLVPKISVGLGGSLSNERSNSVDQYKIIKKERHAIRPPVKHNWSLPGSNVDAN 480

Query: 481  PPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF 540
            PPQIFQHELLQNFSI MFCK+PVNKV+TFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF
Sbjct: 481  PPQIFQHELLQNFSIYMFCKVPVNKVQTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF 540

Query: 541  TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL 600
            TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL
Sbjct: 541  TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL 600

Query: 601  LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA 660
            LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA
Sbjct: 601  LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA 660

Query: 661  EVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAE 720
            EVLEFISDYGKPT+PLPAAIVACVPKKAH DGLGETEFK+TASEIITAIEAGL+EPIEAE
Sbjct: 661  EVLEFISDYGKPTSPLPAAIVACVPKKAHTDGLGETEFKLTASEIITAIEAGLEEPIEAE 720

Query: 721  PELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKI 780
            PELEVPKELISSSQIAELRMQ KPSFIP NPET+VTKFHDKETGITQCRLSNGIPVNYKI
Sbjct: 721  PELEVPKELISSSQIAELRMQHKPSFIPSNPETNVTKFHDKETGITQCRLSNGIPVNYKI 780

Query: 781  SKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLIN 840
            SKSENK+GVMRLIVGGGRAAESPD QGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLIN
Sbjct: 781  SKSENKSGVMRLIVGGGRAAESPDLQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLIN 840

Query: 841  CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQLY 900
            CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE         HSVWLEDAFDRAKQLY
Sbjct: 841  CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE---------HSVWLEDAFDRAKQLY 900

Query: 901  MSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNME 960
            MSYY SIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVK+AVMNQFVGNNME
Sbjct: 901  MSYYWSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKDAVMNQFVGNNME 960

Query: 961  VSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTDER 1020
            VS+VGDFSEEE+ESCILDYLGTVTA T  E AP SVPIVFRPSPSELQFQQVFLKDTDER
Sbjct: 961  VSVVGDFSEEEIESCILDYLGTVTAKTPCETAPASVPIVFRPSPSELQFQQVFLKDTDER 1020

Query: 1021 ACAYISGPAPNRWGVTVDGLELLETVSHISRTDVNDAFDNDIEKGLQRKLRSHPLFFGIT 1080
            ACAYISGPAPNRWGVTV+G+ELLE+VS ISRTD ++  DNDIEKGLQRKLRSHPLFFGIT
Sbjct: 1021 ACAYISGPAPNRWGVTVEGVELLESVSQISRTDESEESDNDIEKGLQRKLRSHPLFFGIT 1080

Query: 1081 MGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTSTPG 1140
            MGLLAEIINSR          LFT+VRDSLGLTYDVSFE+SLFDRLKLGWYVISVTSTP 
Sbjct: 1081 MGLLAEIINSR----------LFTNVRDSLGLTYDVSFELSLFDRLKLGWYVISVTSTPA 1140

Query: 1141 KVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP 1200
            KVYKAVDACKNVLRGLH NKI+QRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP
Sbjct: 1141 KVYKAVDACKNVLRGLHGNKISQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP 1200

Query: 1201 RKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEEEGS 1260
            RKDLSCIKDLTSLYEAATIDD+YIAYDQLKVDADSLYTCIG+AGAQAGEE+I  FEEEGS
Sbjct: 1201 RKDLSCIKDLTSLYEAATIDDVYIAYDQLKVDADSLYTCIGVAGAQAGEESIVPFEEEGS 1260

Query: 1261 DQDFQGVVPTGRGLSTMTRPTT 1282
            DQDFQGVVPTGRGLSTMTRPTT
Sbjct: 1261 DQDFQGVVPTGRGLSTMTRPTT 1263

BLAST of MS022004 vs. NCBI nr
Match: XP_022965458.1 (stromal processing peptidase, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022965459.1 stromal processing peptidase, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 2295.8 bits (5948), Expect = 0.0e+00
Identity = 1179/1284 (91.82%), Postives = 1214/1284 (94.55%), Query Frame = 0

Query: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRS-CTPLARFNVDSRFVVSLRR 60
            MAVASSSTVSNLTQRRPLLSL+D G PNRR NS++LP RS C+ LARF+V+SRFVV LRR
Sbjct: 1    MAVASSSTVSNLTQRRPLLSLRDPGTPNRRANSVQLPSRSICSNLARFDVESRFVVPLRR 60

Query: 61   HSHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRF--T 120
            +SHDDGIGRYKFRRNKDNARRP A KI ERG+ TS T NCISC LNQKR    IKRF  T
Sbjct: 61   YSHDDGIGRYKFRRNKDNARRPHAYKIGERGSGTSETTNCISCFLNQKRRGPGIKRFTPT 120

Query: 121  PRFIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPE 180
            PRFIFDKSA +LSKNE   KVVKHA IVC TVGPDEPHAA T WPDGILEKQDLD SYPE
Sbjct: 121  PRFIFDKSAFQLSKNECGVKVVKHARIVCGTVGPDEPHAATTDWPDGILEKQDLDASYPE 180

Query: 181  FGRAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDE 240
            FGRAELEAFLSSELPSHPKL+RGQLKNGL+YLILPNKVPPNRFEAHMEVHVGSIDEEDDE
Sbjct: 181  FGRAELEAFLSSELPSHPKLHRGQLKNGLKYLILPNKVPPNRFEAHMEVHVGSIDEEDDE 240

Query: 241  QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLD 300
            QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLD
Sbjct: 241  QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLD 300

Query: 301  ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE 360
            ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE
Sbjct: 301  ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE 360

Query: 361  EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPN 420
            EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNI+KTVNQIEAVF +TG+E EAVSTPN
Sbjct: 361  EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNISKTVNQIEAVFDDTGLEKEAVSTPN 420

Query: 421  PSAFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVD 480
            PSAFGAMASFLVPKISVGL GSLSNERSNSVDQSKIIKKERH IRPPVKHNWSLPGSNV+
Sbjct: 421  PSAFGAMASFLVPKISVGLGGSLSNERSNSVDQSKIIKKERHAIRPPVKHNWSLPGSNVN 480

Query: 481  TNPPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNP 540
             NPPQIFQHELLQNFSI MFCK+PVNKV+TFSDLRNVLMKRIFLSALHFRINTRYKSSNP
Sbjct: 481  ANPPQIFQHELLQNFSIYMFCKVPVNKVQTFSDLRNVLMKRIFLSALHFRINTRYKSSNP 540

Query: 541  PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD 600
            PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD
Sbjct: 541  PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD 600

Query: 601  ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI 660
            ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI
Sbjct: 601  ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI 660

Query: 661  GAEVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIE 720
            GAEVLEFISDYGKPT+PLPAAIVACVPKKAHIDGLGETEFK+TASEIITAIEAGL+EPIE
Sbjct: 661  GAEVLEFISDYGKPTSPLPAAIVACVPKKAHIDGLGETEFKLTASEIITAIEAGLEEPIE 720

Query: 721  AEPELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNY 780
            AEPELEVPKELISSSQIAELRMQ KPSFIP NPET+VTKFHDKETGITQCRLSNGIPVNY
Sbjct: 721  AEPELEVPKELISSSQIAELRMQHKPSFIPSNPETNVTKFHDKETGITQCRLSNGIPVNY 780

Query: 781  KISKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL 840
            KISKSENK+GVMRLIVGGGRAAESPD QGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL
Sbjct: 781  KISKSENKSGVMRLIVGGGRAAESPDLQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL 840

Query: 841  INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQ 900
            INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE         HSVWLEDAFDRAKQ
Sbjct: 841  INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE---------HSVWLEDAFDRAKQ 900

Query: 901  LYMSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNN 960
            LYMSYY SIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVK+AVMNQFVGNN
Sbjct: 901  LYMSYYWSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKDAVMNQFVGNN 960

Query: 961  MEVSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTD 1020
            MEVS+VGDFSEEE+ESCILDYLGTVTA T  E AP SVPIVFRPSPSELQFQQVFLKDTD
Sbjct: 961  MEVSVVGDFSEEEIESCILDYLGTVTAKTPCELAPASVPIVFRPSPSELQFQQVFLKDTD 1020

Query: 1021 ERACAYISGPAPNRWGVTVDGLELLETVSHISRTDVNDAFDNDIEKGLQRKLRSHPLFFG 1080
            ERACAYISGPAPNRWGVTV+G+ELLE+VS ISRTD +D  DNDIEKGLQRKLRSHPLFFG
Sbjct: 1021 ERACAYISGPAPNRWGVTVEGVELLESVSQISRTDESDESDNDIEKGLQRKLRSHPLFFG 1080

Query: 1081 ITMGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTST 1140
            ITMGLLAEIINSR          LFT+VRDSLGLTYDVSFE+SLFDRLKLGWYVISVTST
Sbjct: 1081 ITMGLLAEIINSR----------LFTNVRDSLGLTYDVSFELSLFDRLKLGWYVISVTST 1140

Query: 1141 PGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS 1200
            P KVYKAVDACKNVLRGLH NKI+QRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS
Sbjct: 1141 PAKVYKAVDACKNVLRGLHGNKISQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS 1200

Query: 1201 VPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEEE 1260
            VPRKDLSCIKDLTSLYEAATIDD+YIAYDQLKVDADSLYTCIG+AGAQAGEE+I SFEEE
Sbjct: 1201 VPRKDLSCIKDLTSLYEAATIDDVYIAYDQLKVDADSLYTCIGVAGAQAGEESIVSFEEE 1260

Query: 1261 GSDQDFQGVVPTGRGLSTMTRPTT 1282
            GSDQDFQGVVPTGRGLSTMTRPTT
Sbjct: 1261 GSDQDFQGVVPTGRGLSTMTRPTT 1265

BLAST of MS022004 vs. NCBI nr
Match: XP_022938034.1 (stromal processing peptidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 2294.2 bits (5944), Expect = 0.0e+00
Identity = 1177/1284 (91.67%), Postives = 1212/1284 (94.39%), Query Frame = 0

Query: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRS-CTPLARFNVDSRFVVSLRR 60
            MAVA+SSTVSNLTQRRPLLSL+D G PNRR NS++LP RS C+ LARF+V+SRFVV LRR
Sbjct: 1    MAVATSSTVSNLTQRRPLLSLRDPGTPNRRANSVQLPSRSICSNLARFDVESRFVVPLRR 60

Query: 61   HSHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRF--T 120
            +SHDDG GRYKFRRNKDNARRP A KI ERG+ TS T NCISC LNQKR    IKRF  T
Sbjct: 61   YSHDDGTGRYKFRRNKDNARRPHAYKIGERGSGTSETTNCISCFLNQKRRGPGIKRFTPT 120

Query: 121  PRFIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPE 180
            PRFIFDKS  +LSKNE D KVVKHA IVC TVGPDEPHAA T WPDGILEKQDLD SYPE
Sbjct: 121  PRFIFDKSVFQLSKNERDVKVVKHARIVCGTVGPDEPHAATTDWPDGILEKQDLDASYPE 180

Query: 181  FGRAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDE 240
            FGRAELEAFLSSELPSHPKL+RGQLKNGL+YLILPNKVPPNRFEAHMEVHVGSIDEEDDE
Sbjct: 181  FGRAELEAFLSSELPSHPKLHRGQLKNGLKYLILPNKVPPNRFEAHMEVHVGSIDEEDDE 240

Query: 241  QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLD 300
            QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPT TKDS+GDLLPSVLD
Sbjct: 241  QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTITKDSEGDLLPSVLD 300

Query: 301  ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE 360
            ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE
Sbjct: 301  ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE 360

Query: 361  EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPN 420
            EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNI+KTVNQIEAVF +TG+E EAVSTPN
Sbjct: 361  EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNISKTVNQIEAVFDDTGLEKEAVSTPN 420

Query: 421  PSAFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVD 480
            PSAFGAMASFLVPKISVGL GSLSNERSNSVDQSKIIKKERH IRPPVKHNWSLPGSNV+
Sbjct: 421  PSAFGAMASFLVPKISVGLGGSLSNERSNSVDQSKIIKKERHAIRPPVKHNWSLPGSNVN 480

Query: 481  TNPPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNP 540
             NPPQIFQHELLQNFSI MFCK+PVNKV+TFSDLRNVLMKRIFLSALHFRINTRYKSSNP
Sbjct: 481  ANPPQIFQHELLQNFSIYMFCKVPVNKVQTFSDLRNVLMKRIFLSALHFRINTRYKSSNP 540

Query: 541  PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD 600
            PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD
Sbjct: 541  PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD 600

Query: 601  ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI 660
            ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI
Sbjct: 601  ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI 660

Query: 661  GAEVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIE 720
            GAEVLEFISDYGKPT+PLPAAIVACVPKKAHIDGLGETEFK+TASEIITAIEAGL+EPIE
Sbjct: 661  GAEVLEFISDYGKPTSPLPAAIVACVPKKAHIDGLGETEFKLTASEIITAIEAGLEEPIE 720

Query: 721  AEPELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNY 780
            AEPELEVPKELISSSQIAELRMQ KPSFIP NPETSVTKFHDKETGITQCRLSNGIPVNY
Sbjct: 721  AEPELEVPKELISSSQIAELRMQHKPSFIPSNPETSVTKFHDKETGITQCRLSNGIPVNY 780

Query: 781  KISKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL 840
            KISKSENK+GVMRLIVGGGRAAESPD QGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL
Sbjct: 781  KISKSENKSGVMRLIVGGGRAAESPDLQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL 840

Query: 841  INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQ 900
            INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE         HSVWLEDAFDRAKQ
Sbjct: 841  INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE---------HSVWLEDAFDRAKQ 900

Query: 901  LYMSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNN 960
            LYMSYY SIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVK+AVMNQFVGNN
Sbjct: 901  LYMSYYWSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKDAVMNQFVGNN 960

Query: 961  MEVSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTD 1020
            MEVS+VGDFSEEE+ESCILDYLGTVTA T  E AP SVPIVFRPSPSELQFQQVFLKDTD
Sbjct: 961  MEVSVVGDFSEEEIESCILDYLGTVTAKTPCETAPASVPIVFRPSPSELQFQQVFLKDTD 1020

Query: 1021 ERACAYISGPAPNRWGVTVDGLELLETVSHISRTDVNDAFDNDIEKGLQRKLRSHPLFFG 1080
            ERACAYISGPAPNRWGVTVDG+ELLE+VS ISRTD +D  DNDIEKGLQRKLRSHPLFFG
Sbjct: 1021 ERACAYISGPAPNRWGVTVDGVELLESVSQISRTDESDESDNDIEKGLQRKLRSHPLFFG 1080

Query: 1081 ITMGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTST 1140
            ITMGLLAEIINSR          LFT+VRDSLGLTYDVSFE+SLFDRLKLGWYVISVTST
Sbjct: 1081 ITMGLLAEIINSR----------LFTNVRDSLGLTYDVSFELSLFDRLKLGWYVISVTST 1140

Query: 1141 PGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS 1200
            P KVYKAVDACKNVLRGLH NKI+QRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS
Sbjct: 1141 PAKVYKAVDACKNVLRGLHGNKISQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS 1200

Query: 1201 VPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEEE 1260
            VPRKDLSCIKDLTSLYEAATIDD+YIAYDQLKVDADSLYTCIG+AGAQAGEE+I SFEEE
Sbjct: 1201 VPRKDLSCIKDLTSLYEAATIDDVYIAYDQLKVDADSLYTCIGVAGAQAGEESIVSFEEE 1260

Query: 1261 GSDQDFQGVVPTGRGLSTMTRPTT 1282
            GSDQDFQGVVPTGRGLSTMTRPTT
Sbjct: 1261 GSDQDFQGVVPTGRGLSTMTRPTT 1265

BLAST of MS022004 vs. ExPASy Swiss-Prot
Match: Q9FIH8 (Stromal processing peptidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SPP PE=2 SV=1)

HSP 1 Score: 1813.5 bits (4696), Expect = 0.0e+00
Identity = 944/1293 (73.01%), Postives = 1081/1293 (83.60%), Query Frame = 0

Query: 4    ASSSTVSNLTQRRPLLSLKDSGAPNR------RLNSLRLPPRS--CTPLARFNVDSRFVV 63
            +SSS++    +  P+L+  +SG   R        N +R  P S   TP  R  V++  ++
Sbjct: 3    SSSSSIFTGVKFSPILAPFNSGDSRRSRYLKDSRNKVRFNPSSPRLTP-HRVRVEAPSLI 62

Query: 64   SLRRHSHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKR 123
                    +G+   +   +K   +R   S     G   S  RN    CL  KR ++ I+R
Sbjct: 63   PY------NGLWAAQPNSHKGRLKRNIVSGKEATGISLSQGRN---FCLTCKRNQAGIRR 122

Query: 124  FTPRFIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGIL-EKQDLDIS 183
              P    D++A  LS++     + KH+ IV AT+GPDEPHAA TAWPDGI+ E+QDLD+ 
Sbjct: 123  ALPSAFVDRTAFSLSRSSLTSSLRKHSQIVNATLGPDEPHAAGTAWPDGIVAERQDLDLL 182

Query: 184  YPEFGRAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEE 243
             PE   AELEAFL  ELPSHPKL+RGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEE
Sbjct: 183  PPEIDSAELEAFLGCELPSHPKLHRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEE 242

Query: 244  DDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPS 303
            +DEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPT TKDS+ DL PS
Sbjct: 243  EDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTHTKDSEDDLFPS 302

Query: 304  VLDALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPI 363
            VLDALNEIAFHPKFL+SRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKL RRFPI
Sbjct: 303  VLDALNEIAFHPKFLSSRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLGRRFPI 362

Query: 364  GLEEQIKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAV- 423
            GLEEQIKKWD DKIRKFHERWYFPANATLYIVGDIDNI + V+ IEAVF + G++NE+  
Sbjct: 363  GLEEQIKKWDVDKIRKFHERWYFPANATLYIVGDIDNIPRIVHNIEAVFGKNGLDNESTP 422

Query: 424  STPNPSAFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPG 483
            S+P+P AFGAMA+FLVPK+  GL G+ SNE++N+ DQSK+IK+ERH IRPPV+HNWSLPG
Sbjct: 423  SSPSPGAFGAMANFLVPKLPAGLGGTFSNEKTNTADQSKMIKRERHAIRPPVEHNWSLPG 482

Query: 484  SNVDTNPPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYK 543
            ++VD  PPQIF+HELLQNF+INMFCKIPV+KV+TF DLRNVLMKRIFLSALHFRINTRYK
Sbjct: 483  TSVDLKPPQIFKHELLQNFAINMFCKIPVSKVQTFGDLRNVLMKRIFLSALHFRINTRYK 542

Query: 544  SSNPPFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELT 603
            SSNPPFTS+ELDHSDSGREGCTVTTLTVTAEP+NWQ+A+KVAVQEVRRLKEFGVT+GELT
Sbjct: 543  SSNPPFTSVELDHSDSGREGCTVTTLTVTAEPQNWQNAVKVAVQEVRRLKEFGVTRGELT 602

Query: 604  RYMDALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEE 663
            RYMDALLKDSEHLAAMIDNVSSVDNLDFIMESDAL HTVMDQ QGHE+LVAVAGTVTLEE
Sbjct: 603  RYMDALLKDSEHLAAMIDNVSSVDNLDFIMESDALSHTVMDQTQGHETLVAVAGTVTLEE 662

Query: 664  VNSIGAEVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLD 723
            VN++GA+VLEFISD+G+PTAPLPAAIVACVP K H+DG+GE++F I+  EII ++++GL 
Sbjct: 663  VNTVGAKVLEFISDFGRPTAPLPAAIVACVPTKVHVDGVGESDFNISPDEIIESVKSGLL 722

Query: 724  EPIEAEPELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGI 783
             PIEAEPELEVPKELIS SQ+ EL +Q+ P F+P+ P + +TK HDKETGITQ RLSNGI
Sbjct: 723  APIEAEPELEVPKELISQSQLKELTLQRNPCFVPI-PGSGLTKLHDKETGITQLRLSNGI 782

Query: 784  PVNYKISKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFC 843
             VNYK S +E++AGVMRLIVGGGRAAE+ DS+GAVVVGVRTLSEGGRVG FSREQVELFC
Sbjct: 783  AVNYKKSTTESRAGVMRLIVGGGRAAETSDSKGAVVVGVRTLSEGGRVGDFSREQVELFC 842

Query: 844  VNHLINCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFD 903
            VNHLINCSLESTEEFIAMEFRFTLRDNGM+AAFQLLHMVLE          SVWLEDAFD
Sbjct: 843  VNHLINCSLESTEEFIAMEFRFTLRDNGMQAAFQLLHMVLE---------RSVWLEDAFD 902

Query: 904  RAKQLYMSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQF 963
            RA+QLY+SY+RSIPKSLER+TAHKLM+AMLNGDERFVEP+PKSLQ+L L++VK+AVM+ F
Sbjct: 903  RARQLYLSYFRSIPKSLERATAHKLMIAMLNGDERFVEPTPKSLQSLNLESVKDAVMSHF 962

Query: 964  VGNNMEVSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFL 1023
            VG+NMEVS+VGDFSEEE+E CILDYLGTV A+ +S + P S PI+FR   + LQFQQVFL
Sbjct: 963  VGDNMEVSIVGDFSEEEIERCILDYLGTVKASHDSAKPPGSEPILFRQPTAGLQFQQVFL 1022

Query: 1024 KDTDERACAYISGPAPNRWGVTVDGLELLETVSH--ISRTDVNDAFDNDIEKG---LQRK 1083
            KDTDERACAYI+GPAPNRWG TVDG +L ++VS   ++   +  + +  +E G   LQ+K
Sbjct: 1023 KDTDERACAYIAGPAPNRWGFTVDGDDLFQSVSKLPVAHDGLLKSEEQLLEGGDRELQKK 1082

Query: 1084 LRSHPLFFGITMGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLG 1143
            LR+HPLFFG+TMGLLAEIINSR          LFT+VRDSLGLTYDVSFE++LFDRL LG
Sbjct: 1083 LRAHPLFFGVTMGLLAEIINSR----------LFTTVRDSLGLTYDVSFELNLFDRLNLG 1142

Query: 1144 WYVISVTSTPGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLG 1203
            WYVISVTSTPGKVYKAVDACK+VLRGLHSN+IA RELDRAKRTLLMRHEAE+KSNAYWL 
Sbjct: 1143 WYVISVTSTPGKVYKAVDACKSVLRGLHSNQIAPRELDRAKRTLLMRHEAELKSNAYWLN 1202

Query: 1204 LLAHLQASSVPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGE 1263
            LLAHLQASSVPRK+LSCIK+L SLYEAA+I+DIY+AY+QL+VD DSLY+CIGIAGAQAGE
Sbjct: 1203 LLAHLQASSVPRKELSCIKELVSLYEAASIEDIYLAYNQLRVDEDSLYSCIGIAGAQAGE 1262

Query: 1264 ENIGSFEEEGSDQDFQGVVPTGRGLSTMTRPTT 1282
            E     EEE  +  F GVVP GRG S  TRPTT
Sbjct: 1263 EITVLSEEEEPEDVFSGVVPVGRGSSMTTRPTT 1265

BLAST of MS022004 vs. ExPASy Swiss-Prot
Match: Q40983 (Stromal processing peptidase, chloroplastic OS=Pisum sativum OX=3888 GN=SPP PE=1 SV=2)

HSP 1 Score: 1803.1 bits (4669), Expect = 0.0e+00
Identity = 935/1286 (72.71%), Postives = 1064/1286 (82.74%), Query Frame = 0

Query: 3    VASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRSCTPLA--RFNVDSRFVVSLRRH 62
            +A+S++ S+L+     LSL     P+R       P    T +   R  + S    S  R 
Sbjct: 1    MAASTSTSSLSVVGTNLSL----PPHRHHRHFHSPSSISTRIRTNRLFLSSSLAFSSPRD 60

Query: 63   SH--DDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCL-NQKRGRSSIKRFT 122
            +     G+G    RRN  +  +  +S + +        ++C SCCL + K+ RS++ RF 
Sbjct: 61   ARVVHAGLG---LRRNTPDVWKHYSSVLSQPTAPVPVRQSCTSCCLASAKKRRSNLPRFV 120

Query: 123  PRFIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPE 182
            P   FD S+  LSK++     VK   +  ATVGPDEPHAA+T W +G+ EKQDL +   E
Sbjct: 121  PGAFFDSSSFGLSKDKLRHASVKRVQLPHATVGPDEPHAASTTWQEGVAEKQDLSLFDSE 180

Query: 183  FGRAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDE 242
              R  LE FL SELPSHPKL+RGQLKNG+RYLILPNKVPP RFEAHMEVHVGSIDEEDDE
Sbjct: 181  LER--LEGFLGSELPSHPKLHRGQLKNGIRYLILPNKVPPTRFEAHMEVHVGSIDEEDDE 240

Query: 243  QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLD 302
            QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSD DLLPSVLD
Sbjct: 241  QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSD-DLLPSVLD 300

Query: 303  ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE 362
            ALNEI FHP FLASR+EKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLS+RFPIGLE
Sbjct: 301  ALNEITFHPNFLASRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLE 360

Query: 363  EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPN 422
            EQIKKWDADKIRKFHERWYFPANATLYIVGDI NI KTVNQIEAVF +TGV+NE  S   
Sbjct: 361  EQIKKWDADKIRKFHERWYFPANATLYIVGDIGNIPKTVNQIEAVFGQTGVDNEKGSVAT 420

Query: 423  PSAFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVD 482
             SAFGAMASFLVPK+SVGL G+     +N+ DQSK+ KKERH +RPPVKH WSLPGS+ +
Sbjct: 421  SSAFGAMASFLVPKLSVGLGGNSIERPTNTTDQSKVFKKERHAVRPPVKHTWSLPGSSAN 480

Query: 483  TNPPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNP 542
              PPQIFQHELLQNFSINMFCKIPVNKV+T+ DLR VLMKRIFLSALHFRINTRYKSSNP
Sbjct: 481  LKPPQIFQHELLQNFSINMFCKIPVNKVQTYRDLRIVLMKRIFLSALHFRINTRYKSSNP 540

Query: 543  PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD 602
            PFTS+ELDHSDSGREGCTVTTLT+TAEPKNWQ+AI+VAV EVRRLKEFGVT+GELTRY+D
Sbjct: 541  PFTSVELDHSDSGREGCTVTTLTITAEPKNWQNAIRVAVHEVRRLKEFGVTQGELTRYLD 600

Query: 603  ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI 662
            ALL+DSEHLAAMIDNVSSVDNLDFIMESDALGH VMDQ QGHESL+AVAGTVTL+EVNS+
Sbjct: 601  ALLRDSEHLAAMIDNVSSVDNLDFIMESDALGHKVMDQSQGHESLIAVAGTVTLDEVNSV 660

Query: 663  GAEVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIE 722
            GA+VLEFI+D+GK +APLPAAIVACVPKK HI+G GETEFKI+++EI  A++AGLDEPIE
Sbjct: 661  GAQVLEFIADFGKLSAPLPAAIVACVPKKVHIEGAGETEFKISSTEITDAMKAGLDEPIE 720

Query: 723  AEPELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNY 782
             EPELEVPKEL+ SS + EL+ Q+KP+FIP++PE    K HD+ETGIT+ RL+NGIPVNY
Sbjct: 721  PEPELEVPKELVQSSTLQELKNQRKPAFIPVSPEIEAKKLHDEETGITRLRLANGIPVNY 780

Query: 783  KISKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL 842
            KISKSE ++GVMRLIVGGGRAAE  DS+G+V+VGVRTLSEGGRVG+FSREQVELFCVN+ 
Sbjct: 781  KISKSETQSGVMRLIVGGGRAAEGSDSRGSVIVGVRTLSEGGRVGNFSREQVELFCVNNQ 840

Query: 843  INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQ 902
            INCSLESTEEFI++EFRFTLR+NGMRAAFQLLHMVLE         HSVW +DA DRA+Q
Sbjct: 841  INCSLESTEEFISLEFRFTLRNNGMRAAFQLLHMVLE---------HSVWSDDALDRARQ 900

Query: 903  LYMSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNN 962
            +Y+SYYRSIPKSLERSTAHKLM+AML+GDERF EP+P SL+NLTLQ+VK+AVMNQFVGNN
Sbjct: 901  VYLSYYRSIPKSLERSTAHKLMVAMLDGDERFTEPTPSSLENLTLQSVKDAVMNQFVGNN 960

Query: 963  MEVSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTD 1022
            MEVS+VGDF+EEE+ESCILDYLGT  AT N +     +P  FR SPS LQ Q+VFL DTD
Sbjct: 961  MEVSIVGDFTEEEIESCILDYLGTAQATGNFKNQQQIIPPTFRLSPSSLQSQEVFLNDTD 1020

Query: 1023 ERACAYISGPAPNRWGVTVDGLELLETVSHISRTDVNDAFDNDI--EKGLQRKLRSHPLF 1082
            ERACAYI+GPAPNRWG T DG +LLET+ + S  + N    + +  E   +R LRSHPLF
Sbjct: 1021 ERACAYIAGPAPNRWGFTADGNDLLETIDNASSVNNNGTKSDALQTEGAPRRSLRSHPLF 1080

Query: 1083 FGITMGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVT 1142
            FGITMGLL+EIINSR          LFT+VRDSLGLTYDVSFE++LFDRLKLGWYV+SVT
Sbjct: 1081 FGITMGLLSEIINSR----------LFTTVRDSLGLTYDVSFELNLFDRLKLGWYVVSVT 1140

Query: 1143 STPGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQA 1202
            STP KV+KAVDACKNVLRGLHSN I  RELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQ+
Sbjct: 1141 STPSKVHKAVDACKNVLRGLHSNGITVRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQS 1200

Query: 1203 SSVPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFE 1262
            SSVPRKDLSCIKDLTSLYEAATI+D  +AY+QLKVD DSLY+CIG++GAQA ++     E
Sbjct: 1201 SSVPRKDLSCIKDLTSLYEAATIEDTCLAYEQLKVDEDSLYSCIGVSGAQAAQDIAAPVE 1257

Query: 1263 EEGSDQDFQGVVPTGRGLSTMTRPTT 1282
            EE + + + GV+P GRGLSTMTRPTT
Sbjct: 1261 EEEAGEGYPGVLPMGRGLSTMTRPTT 1257

BLAST of MS022004 vs. ExPASy Swiss-Prot
Match: Q69TY5 (Stromal processing peptidase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SPP PE=2 SV=2)

HSP 1 Score: 1635.9 bits (4235), Expect = 0.0e+00
Identity = 845/1212 (69.72%), Postives = 976/1212 (80.53%), Query Frame = 0

Query: 73   RNKDNARRPGASKIVERGNETSGTRNCISCC-LNQKRGRSSIKRFTPRFIFDKSALRLSK 132
            R +  A    ++ I   G E  G   C+SC    ++RGR  + RF P  +     L    
Sbjct: 65   RRRTEAVGAASAAIGSLGEEREG---CLSCFPRGRRRGRPGLARFAPCALPHTYGLSSLH 124

Query: 133  NEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPEFGRAELEAFLSSEL 192
            +      ++   ++ A  GPDEPH A+  W +  L+K  +D      G+ ELE FL++ L
Sbjct: 125  SGLTGAKIRRRHVLHA-AGPDEPHVASPTWSETALDKHYVD---QPIGKEELEGFLNTPL 184

Query: 193  PSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLG 252
            PSHPKL RGQLKNGLRYLILPNKVP NRFEAHMEVHVGSIDEE+DEQGIAHMIEHVAFLG
Sbjct: 185  PSHPKLVRGQLKNGLRYLILPNKVPANRFEAHMEVHVGSIDEEEDEQGIAHMIEHVAFLG 244

Query: 253  SKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDALNEIAFHPKFLAS 312
            SKKREKLLGTGARSNAYTDFHHTVFHIHSPT TK+   DLLPSVLDALNEIAFHPKF +S
Sbjct: 245  SKKREKLLGTGARSNAYTDFHHTVFHIHSPTKTKEYGEDLLPSVLDALNEIAFHPKFSSS 304

Query: 313  RVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQIKKWDADKIRKF 372
            RVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLS RFPIGLEEQI KWD DKIR+F
Sbjct: 305  RVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSERFPIGLEEQIHKWDPDKIRRF 364

Query: 373  HERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPSAFGAMASFLVPK 432
            HERWY+PANATLY+VG+ID+I + + +IEAVF  T  E EA      S FGAMAS   PK
Sbjct: 365  HERWYYPANATLYLVGEIDDIPRAIREIEAVFEHTLPEGEAAPMSTASPFGAMASLFAPK 424

Query: 433  ISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVDTNPPQIFQHELLQN 492
            +  GL  SL+ ERS + D+ K +K+ER  IRPPV+H WSLPG   D  PP IFQHEL+Q+
Sbjct: 425  LPGGLAASLTGERSPAADKIKPVKRERQAIRPPVEHKWSLPGVAQDAKPPAIFQHELIQS 484

Query: 493  FSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPFTSIELDHSDSGR 552
            FSINMFCKIPVN+V+T+ DLR+VLMKRIFLSALHFRINTRYKSSNPPFTS+ELDHSDSGR
Sbjct: 485  FSINMFCKIPVNQVQTYKDLRSVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGR 544

Query: 553  EGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDALLKDSEHLAAMID 612
            EGCTVTTLTVTAEP+NW+SAIKVAV EVRRLKEFGVT GE+TRYMDAL+KDSE LA MID
Sbjct: 545  EGCTVTTLTVTAEPQNWRSAIKVAVHEVRRLKEFGVTMGEMTRYMDALIKDSEQLAMMID 604

Query: 613  NVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGAEVLEFISDYGKP 672
            +V SVDNLDFIMESDAL HTVMDQ QGHESL+AVA TVTLEEVN++GAEVLEFISDYGKP
Sbjct: 605  SVPSVDNLDFIMESDALRHTVMDQLQGHESLLAVAETVTLEEVNTVGAEVLEFISDYGKP 664

Query: 673  TAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAEPELEVPKELISS 732
             APLPAAIVACVPKK H+DG+GET+F+I   EI  +I+AGL+EPI  EPELEVPKELI+ 
Sbjct: 665  DAPLPAAIVACVPKKVHMDGVGETDFEIHPEEITDSIKAGLEEPIYPEPELEVPKELITR 724

Query: 733  SQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKISKSENKAGVMRL 792
            S++ +L++Q+KPSF  L+ E +V K  D ETGI Q RLSNGI +NYKI+++E + GVMRL
Sbjct: 725  SELEDLKLQRKPSFASLSKEENVVKIFDDETGIAQRRLSNGISINYKITQNEARVGVMRL 784

Query: 793  IVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINCSLESTEEFIAM 852
            IVGGGRA E  +S+G+V+VGVRTLSEGG VG+FSREQVELFCVN+LINCSLES EEFI M
Sbjct: 785  IVGGGRATEDSESKGSVIVGVRTLSEGGCVGNFSREQVELFCVNNLINCSLESNEEFIFM 844

Query: 853  EFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQLYMSYYRSIPKSLE 912
            EFRF LRDNGMRAAFQLLHMVLE         H+VWLEDAFDRA QLY+SYYRSIPKSLE
Sbjct: 845  EFRFALRDNGMRAAFQLLHMVLE---------HNVWLEDAFDRATQLYLSYYRSIPKSLE 904

Query: 913  RSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNMEVSLVGDFSEEEV 972
            RSTAHKLMLAMLN DERFVEPSP SLQ LTLQ+VK+AVMNQFVG+NMEVS+VGDF+EEEV
Sbjct: 905  RSTAHKLMLAMLNHDERFVEPSPHSLQKLTLQSVKDAVMNQFVGDNMEVSIVGDFTEEEV 964

Query: 973  ESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTDERACAYISGPAPNR 1032
            ESC+LDYLGTV+A  +S+       I F P PS+L FQQV++KDTDERACAYI+GPAPNR
Sbjct: 965  ESCVLDYLGTVSAPKSSKTQEHIEKISFLPFPSDLHFQQVYIKDTDERACAYIAGPAPNR 1024

Query: 1033 WGVTVDGLELLETV-SHISRTDVNDAFDNDIEKGLQRKLRSHPLFFGITMGLLAEIINSR 1092
            WG   +G +L   + S      V+++ + D+ +     +RSH LFFGIT+ LLAEIINSR
Sbjct: 1025 WGFATEGNDLFNVIRSSSGDAQVSESANTDLTERKHNDVRSHSLFFGITLSLLAEIINSR 1084

Query: 1093 QVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTSTPGKVYKAVDACKN 1152
                      LFT+VRDS+GLTYDVSFE++LFD+L LGWYVI+VTSTP KV+KAVDACK 
Sbjct: 1085 ----------LFTTVRDSMGLTYDVSFELNLFDKLDLGWYVIAVTSTPSKVHKAVDACKG 1144

Query: 1153 VLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDLSCIKDLT 1212
            VLRGLHSNKI +RELDRAKRTLLM+HEAE K+NAYWLGLLAHLQ+SSVPRK++SCIK+LT
Sbjct: 1145 VLRGLHSNKIVERELDRAKRTLLMKHEAETKTNAYWLGLLAHLQSSSVPRKEISCIKELT 1204

Query: 1213 SLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEEEGSDQDFQGVVPT- 1272
             LYE+ATI+D+Y+AY+ LKVD  SL+ CIGIAGA++GEE      ++  D    G+ P  
Sbjct: 1205 MLYESATIEDLYLAYEHLKVDESSLFACIGIAGAESGEET----TDDELDMGLHGMGPIG 1246

Query: 1273 GRGLSTMTRPTT 1282
            GRGLSTMTRPTT
Sbjct: 1265 GRGLSTMTRPTT 1246

BLAST of MS022004 vs. ExPASy Swiss-Prot
Match: B8B0E2 (Stromal processing peptidase, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_23765 PE=1 SV=2)

HSP 1 Score: 1634.4 bits (4231), Expect = 0.0e+00
Identity = 844/1212 (69.64%), Postives = 976/1212 (80.53%), Query Frame = 0

Query: 73   RNKDNARRPGASKIVERGNETSGTRNCISCC-LNQKRGRSSIKRFTPRFIFDKSALRLSK 132
            R +  A    ++ I   G E  G   C+SC    ++RGR  + RF P  +     L    
Sbjct: 65   RRRTEAVGAASAAIGSLGEEREG---CLSCFPRGRRRGRPGLARFAPCALPHTYGLSSLH 124

Query: 133  NEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPEFGRAELEAFLSSEL 192
            +      ++   ++ A  GPDEPH A+  W +  L+K  +D      G+ ELE FL++ L
Sbjct: 125  SGLTGAKIRRRHVLHA-AGPDEPHVASPTWSETALDKHYVD---QPIGKEELEGFLNTPL 184

Query: 193  PSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLG 252
            PSHPKL RGQLKNGLRYLILPNKVP NRFEAHMEVHVGSIDEE+DEQGIAHMIEHVAFLG
Sbjct: 185  PSHPKLVRGQLKNGLRYLILPNKVPANRFEAHMEVHVGSIDEEEDEQGIAHMIEHVAFLG 244

Query: 253  SKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDALNEIAFHPKFLAS 312
            SKKREKLLGTGARSNAYTDFHHTVFHIHSPT TK+   DLLPSVLDALNEIAFHPKF +S
Sbjct: 245  SKKREKLLGTGARSNAYTDFHHTVFHIHSPTKTKEYGEDLLPSVLDALNEIAFHPKFSSS 304

Query: 313  RVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQIKKWDADKIRKF 372
            RVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLS RFPIGLEEQI KWD DKIR+F
Sbjct: 305  RVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSERFPIGLEEQIHKWDPDKIRRF 364

Query: 373  HERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPSAFGAMASFLVPK 432
            HERWY+PANATLY+VG+I++I + + +IEAVF  T  E EA      S FGAMAS   PK
Sbjct: 365  HERWYYPANATLYLVGEINDIPRAIREIEAVFEHTLPEGEAAPMSTASPFGAMASLFAPK 424

Query: 433  ISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVDTNPPQIFQHELLQN 492
            +  GL  SL+ ERS + D+ K +K+ER  IRPPV+H WSLPG   D  PP IFQHEL+Q+
Sbjct: 425  LPGGLAASLTGERSPAADKIKPVKRERQAIRPPVEHKWSLPGVAQDAKPPAIFQHELIQS 484

Query: 493  FSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPFTSIELDHSDSGR 552
            FSINMFCKIPVN+V+T+ DLR+VLMKRIFLSALHFRINTRYKSSNPPFTS+ELDHSDSGR
Sbjct: 485  FSINMFCKIPVNQVQTYKDLRSVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGR 544

Query: 553  EGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDALLKDSEHLAAMID 612
            EGCTVTTLTVTAEP+NW+SAIKVAV EVRRLKEFGVT GE+TRYMDAL+KDSE LA MID
Sbjct: 545  EGCTVTTLTVTAEPQNWRSAIKVAVHEVRRLKEFGVTMGEMTRYMDALIKDSEQLAMMID 604

Query: 613  NVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGAEVLEFISDYGKP 672
            +V SVDNLDFIMESDAL HTVMDQ QGHESL+AVA TVTLEEVN++GAEVLEFISDYGKP
Sbjct: 605  SVPSVDNLDFIMESDALRHTVMDQLQGHESLLAVAETVTLEEVNTVGAEVLEFISDYGKP 664

Query: 673  TAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAEPELEVPKELISS 732
             APLPAAIVACVPKK H+DG+GET+F+I   EI  +I+AGL+EPI  EPELEVPKELI+ 
Sbjct: 665  DAPLPAAIVACVPKKVHMDGVGETDFEIHPEEITDSIKAGLEEPIYPEPELEVPKELITQ 724

Query: 733  SQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKISKSENKAGVMRL 792
            S++ +L++Q+KPSF  L+ E +V K  D ETGI Q RLSNGI +NYKI+++E + GVMRL
Sbjct: 725  SELEDLKLQRKPSFASLSKEENVVKIFDDETGIAQRRLSNGISINYKITQNEARVGVMRL 784

Query: 793  IVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINCSLESTEEFIAM 852
            IVGGGRA E  +S+G+V+VGVRTLSEGG VG+FSREQVELFCVN+LINCSLES EEFI M
Sbjct: 785  IVGGGRATEDSESKGSVIVGVRTLSEGGCVGNFSREQVELFCVNNLINCSLESNEEFIFM 844

Query: 853  EFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQLYMSYYRSIPKSLE 912
            EFRF LRDNGMRAAFQLLHMVLE         H+VWLEDAFDRA QLY+SYYRSIPKSLE
Sbjct: 845  EFRFALRDNGMRAAFQLLHMVLE---------HNVWLEDAFDRATQLYLSYYRSIPKSLE 904

Query: 913  RSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNMEVSLVGDFSEEEV 972
            RSTAHKLMLAMLN DERFVEPSP SLQ LTLQ+VK+AVMNQFVG+NMEVS+VGDF+EEEV
Sbjct: 905  RSTAHKLMLAMLNHDERFVEPSPHSLQKLTLQSVKDAVMNQFVGDNMEVSIVGDFTEEEV 964

Query: 973  ESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTDERACAYISGPAPNR 1032
            ESC+LDYLGTV+A  +S+       I F P PS+L FQQV++KDTDERACAYI+GPAPNR
Sbjct: 965  ESCVLDYLGTVSAPKSSKTQEHIEKISFLPFPSDLHFQQVYIKDTDERACAYIAGPAPNR 1024

Query: 1033 WGVTVDGLELLETV-SHISRTDVNDAFDNDIEKGLQRKLRSHPLFFGITMGLLAEIINSR 1092
            WG   +G +L   + S      V+++ + D+ +     +RSH LFFGIT+ LLAEIINSR
Sbjct: 1025 WGFATEGNDLFNVIRSSSGDAQVSESANTDLTERKHNDVRSHSLFFGITLSLLAEIINSR 1084

Query: 1093 QVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTSTPGKVYKAVDACKN 1152
                      LFT+VRDS+GLTYDVSFE++LFD+L LGWYVI+VTSTP KV+KAVDACK 
Sbjct: 1085 ----------LFTTVRDSMGLTYDVSFELNLFDKLDLGWYVIAVTSTPSKVHKAVDACKG 1144

Query: 1153 VLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDLSCIKDLT 1212
            VLRGLHSNKI +RELDRAKRTLLM+HEAE K+NAYWLGLLAHLQ+SSVPRK++SCIK+LT
Sbjct: 1145 VLRGLHSNKIVERELDRAKRTLLMKHEAETKTNAYWLGLLAHLQSSSVPRKEISCIKELT 1204

Query: 1213 SLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEEEGSDQDFQGVVPT- 1272
             LYE+ATI+D+Y+AY+ LKVD  SL+ CIGIAGA++GEE      ++  D    G+ P  
Sbjct: 1205 MLYESATIEDLYLAYEHLKVDESSLFACIGIAGAESGEET----TDDELDMGLHGMGPIG 1246

Query: 1273 GRGLSTMTRPTT 1282
            GRGLSTMTRPTT
Sbjct: 1265 GRGLSTMTRPTT 1246

BLAST of MS022004 vs. ExPASy Swiss-Prot
Match: P31828 (Probable zinc protease PqqL OS=Escherichia coli (strain K12) OX=83333 GN=pqqL PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 1.3e-25
Identity = 71/215 (33.02%), Postives = 112/215 (52.09%), Query Frame = 0

Query: 187 LSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGIAHMIEH 246
           +++ LP   KL  GQL NGLRY+I P+  P ++    +++H GS+ EED+E G+AH +EH
Sbjct: 25  IAAALPQDEKLITGQLDNGLRYMIYPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEH 84

Query: 247 VAFLGSK--KREKLLGT--------GARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVL 306
           + F G+K     K++ T        G   NAYT +  TV+ +  PT+ K +    L  V+
Sbjct: 85  MMFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQN----LQQVM 144

Query: 307 DALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGL 366
              +E +    F    V+ ER  I  E +     ++R        L +  +   R PIGL
Sbjct: 145 AIFSEWSNAATFEKLEVDAERGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGL 204

Query: 367 EEQIKKWDADKIRKFHERWYFPANATLYIVGDIDN 392
            + +      ++R+F++RWY P N T  +VGDID+
Sbjct: 205 MDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDS 235

BLAST of MS022004 vs. ExPASy TrEMBL
Match: A0A6J1D6U5 (stromal processing peptidase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018166 PE=4 SV=1)

HSP 1 Score: 2451.8 bits (6353), Expect = 0.0e+00
Identity = 1257/1285 (97.82%), Postives = 1257/1285 (97.82%), Query Frame = 0

Query: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRSCTPLARFNVDSRFVVSLRRH 60
            MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRSCTPLARFNVDSRFVVSLRRH
Sbjct: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRSCTPLARFNVDSRFVVSLRRH 60

Query: 61   SHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRFTPRF 120
            SHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRFTPRF
Sbjct: 61   SHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRFTPRF 120

Query: 121  IFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPEFGR 180
            IFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAW DGILEKQDLDISYPEFGR
Sbjct: 121  IFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWSDGILEKQDLDISYPEFGR 180

Query: 181  AELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGI 240
            AELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGI
Sbjct: 181  AELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGI 240

Query: 241  AHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDALN 300
            AHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDALN
Sbjct: 241  AHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDALN 300

Query: 301  EIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQI 360
            EIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQI
Sbjct: 301  EIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQI 360

Query: 361  KKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPSA 420
            KKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPSA
Sbjct: 361  KKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPSA 420

Query: 421  FGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVDTNP 480
            FGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERH IRPPVKHNWSLPGSNV TNP
Sbjct: 421  FGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHAIRPPVKHNWSLPGSNVYTNP 480

Query: 481  PQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPFT 540
            PQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPFT
Sbjct: 481  PQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPFT 540

Query: 541  SIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDALL 600
            SIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDALL
Sbjct: 541  SIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDALL 600

Query: 601  KDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGAE 660
            KDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGAE
Sbjct: 601  KDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGAE 660

Query: 661  VLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAEP 720
            VLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAEP
Sbjct: 661  VLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAEP 720

Query: 721  ELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKIS 780
            ELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKIS
Sbjct: 721  ELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKIS 780

Query: 781  KSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINC 840
            KSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINC
Sbjct: 781  KSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINC 840

Query: 841  SLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQLYM 900
            SLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE         HSVWLEDAFDRAKQLYM
Sbjct: 841  SLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE---------HSVWLEDAFDRAKQLYM 900

Query: 901  SYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNMEV 960
            SYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNMEV
Sbjct: 901  SYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNMEV 960

Query: 961  SLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTDERA 1020
            SLVGDFSEEEVES ILDYLGTVTATTNSERAPPSVPIVFR SPSELQFQQVFLKDTDERA
Sbjct: 961  SLVGDFSEEEVESSILDYLGTVTATTNSERAPPSVPIVFRTSPSELQFQQVFLKDTDERA 1020

Query: 1021 CAYISGPAPNRWGVTVDGLELLETVSHISRTD----VNDAFDNDIEKGLQRKLRSHPLFF 1080
            CAYISGPAPNRWGVTVDGLELLETVSHISRTD    VNDAFDNDIEKGLQRKLRSHPLFF
Sbjct: 1021 CAYISGPAPNRWGVTVDGLELLETVSHISRTDATTPVNDAFDNDIEKGLQRKLRSHPLFF 1080

Query: 1081 GITMGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTS 1140
            GITMGLLAEIINSR          LFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTS
Sbjct: 1081 GITMGLLAEIINSR----------LFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTS 1140

Query: 1141 TPGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQAS 1200
            TPGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQAS
Sbjct: 1141 TPGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQAS 1200

Query: 1201 SVPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEE 1260
            SVPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEE
Sbjct: 1201 SVPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEE 1260

Query: 1261 EGSDQDFQGVVPTGRGLSTMTRPTT 1282
            EGSDQDFQGVVPTGRGLSTMTRPTT
Sbjct: 1261 EGSDQDFQGVVPTGRGLSTMTRPTT 1266

BLAST of MS022004 vs. ExPASy TrEMBL
Match: A0A6J1HNZ8 (stromal processing peptidase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465360 PE=4 SV=1)

HSP 1 Score: 2295.8 bits (5948), Expect = 0.0e+00
Identity = 1179/1284 (91.82%), Postives = 1214/1284 (94.55%), Query Frame = 0

Query: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRS-CTPLARFNVDSRFVVSLRR 60
            MAVASSSTVSNLTQRRPLLSL+D G PNRR NS++LP RS C+ LARF+V+SRFVV LRR
Sbjct: 1    MAVASSSTVSNLTQRRPLLSLRDPGTPNRRANSVQLPSRSICSNLARFDVESRFVVPLRR 60

Query: 61   HSHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRF--T 120
            +SHDDGIGRYKFRRNKDNARRP A KI ERG+ TS T NCISC LNQKR    IKRF  T
Sbjct: 61   YSHDDGIGRYKFRRNKDNARRPHAYKIGERGSGTSETTNCISCFLNQKRRGPGIKRFTPT 120

Query: 121  PRFIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPE 180
            PRFIFDKSA +LSKNE   KVVKHA IVC TVGPDEPHAA T WPDGILEKQDLD SYPE
Sbjct: 121  PRFIFDKSAFQLSKNECGVKVVKHARIVCGTVGPDEPHAATTDWPDGILEKQDLDASYPE 180

Query: 181  FGRAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDE 240
            FGRAELEAFLSSELPSHPKL+RGQLKNGL+YLILPNKVPPNRFEAHMEVHVGSIDEEDDE
Sbjct: 181  FGRAELEAFLSSELPSHPKLHRGQLKNGLKYLILPNKVPPNRFEAHMEVHVGSIDEEDDE 240

Query: 241  QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLD 300
            QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLD
Sbjct: 241  QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLD 300

Query: 301  ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE 360
            ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE
Sbjct: 301  ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE 360

Query: 361  EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPN 420
            EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNI+KTVNQIEAVF +TG+E EAVSTPN
Sbjct: 361  EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNISKTVNQIEAVFDDTGLEKEAVSTPN 420

Query: 421  PSAFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVD 480
            PSAFGAMASFLVPKISVGL GSLSNERSNSVDQSKIIKKERH IRPPVKHNWSLPGSNV+
Sbjct: 421  PSAFGAMASFLVPKISVGLGGSLSNERSNSVDQSKIIKKERHAIRPPVKHNWSLPGSNVN 480

Query: 481  TNPPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNP 540
             NPPQIFQHELLQNFSI MFCK+PVNKV+TFSDLRNVLMKRIFLSALHFRINTRYKSSNP
Sbjct: 481  ANPPQIFQHELLQNFSIYMFCKVPVNKVQTFSDLRNVLMKRIFLSALHFRINTRYKSSNP 540

Query: 541  PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD 600
            PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD
Sbjct: 541  PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD 600

Query: 601  ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI 660
            ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI
Sbjct: 601  ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI 660

Query: 661  GAEVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIE 720
            GAEVLEFISDYGKPT+PLPAAIVACVPKKAHIDGLGETEFK+TASEIITAIEAGL+EPIE
Sbjct: 661  GAEVLEFISDYGKPTSPLPAAIVACVPKKAHIDGLGETEFKLTASEIITAIEAGLEEPIE 720

Query: 721  AEPELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNY 780
            AEPELEVPKELISSSQIAELRMQ KPSFIP NPET+VTKFHDKETGITQCRLSNGIPVNY
Sbjct: 721  AEPELEVPKELISSSQIAELRMQHKPSFIPSNPETNVTKFHDKETGITQCRLSNGIPVNY 780

Query: 781  KISKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL 840
            KISKSENK+GVMRLIVGGGRAAESPD QGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL
Sbjct: 781  KISKSENKSGVMRLIVGGGRAAESPDLQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL 840

Query: 841  INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQ 900
            INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE         HSVWLEDAFDRAKQ
Sbjct: 841  INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE---------HSVWLEDAFDRAKQ 900

Query: 901  LYMSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNN 960
            LYMSYY SIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVK+AVMNQFVGNN
Sbjct: 901  LYMSYYWSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKDAVMNQFVGNN 960

Query: 961  MEVSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTD 1020
            MEVS+VGDFSEEE+ESCILDYLGTVTA T  E AP SVPIVFRPSPSELQFQQVFLKDTD
Sbjct: 961  MEVSVVGDFSEEEIESCILDYLGTVTAKTPCELAPASVPIVFRPSPSELQFQQVFLKDTD 1020

Query: 1021 ERACAYISGPAPNRWGVTVDGLELLETVSHISRTDVNDAFDNDIEKGLQRKLRSHPLFFG 1080
            ERACAYISGPAPNRWGVTV+G+ELLE+VS ISRTD +D  DNDIEKGLQRKLRSHPLFFG
Sbjct: 1021 ERACAYISGPAPNRWGVTVEGVELLESVSQISRTDESDESDNDIEKGLQRKLRSHPLFFG 1080

Query: 1081 ITMGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTST 1140
            ITMGLLAEIINSR          LFT+VRDSLGLTYDVSFE+SLFDRLKLGWYVISVTST
Sbjct: 1081 ITMGLLAEIINSR----------LFTNVRDSLGLTYDVSFELSLFDRLKLGWYVISVTST 1140

Query: 1141 PGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS 1200
            P KVYKAVDACKNVLRGLH NKI+QRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS
Sbjct: 1141 PAKVYKAVDACKNVLRGLHGNKISQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS 1200

Query: 1201 VPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEEE 1260
            VPRKDLSCIKDLTSLYEAATIDD+YIAYDQLKVDADSLYTCIG+AGAQAGEE+I SFEEE
Sbjct: 1201 VPRKDLSCIKDLTSLYEAATIDDVYIAYDQLKVDADSLYTCIGVAGAQAGEESIVSFEEE 1260

Query: 1261 GSDQDFQGVVPTGRGLSTMTRPTT 1282
            GSDQDFQGVVPTGRGLSTMTRPTT
Sbjct: 1261 GSDQDFQGVVPTGRGLSTMTRPTT 1265

BLAST of MS022004 vs. ExPASy TrEMBL
Match: A0A6J1FCW6 (stromal processing peptidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111444241 PE=4 SV=1)

HSP 1 Score: 2294.2 bits (5944), Expect = 0.0e+00
Identity = 1177/1284 (91.67%), Postives = 1212/1284 (94.39%), Query Frame = 0

Query: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRS-CTPLARFNVDSRFVVSLRR 60
            MAVA+SSTVSNLTQRRPLLSL+D G PNRR NS++LP RS C+ LARF+V+SRFVV LRR
Sbjct: 1    MAVATSSTVSNLTQRRPLLSLRDPGTPNRRANSVQLPSRSICSNLARFDVESRFVVPLRR 60

Query: 61   HSHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRF--T 120
            +SHDDG GRYKFRRNKDNARRP A KI ERG+ TS T NCISC LNQKR    IKRF  T
Sbjct: 61   YSHDDGTGRYKFRRNKDNARRPHAYKIGERGSGTSETTNCISCFLNQKRRGPGIKRFTPT 120

Query: 121  PRFIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPE 180
            PRFIFDKS  +LSKNE D KVVKHA IVC TVGPDEPHAA T WPDGILEKQDLD SYPE
Sbjct: 121  PRFIFDKSVFQLSKNERDVKVVKHARIVCGTVGPDEPHAATTDWPDGILEKQDLDASYPE 180

Query: 181  FGRAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDE 240
            FGRAELEAFLSSELPSHPKL+RGQLKNGL+YLILPNKVPPNRFEAHMEVHVGSIDEEDDE
Sbjct: 181  FGRAELEAFLSSELPSHPKLHRGQLKNGLKYLILPNKVPPNRFEAHMEVHVGSIDEEDDE 240

Query: 241  QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLD 300
            QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPT TKDS+GDLLPSVLD
Sbjct: 241  QGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTITKDSEGDLLPSVLD 300

Query: 301  ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE 360
            ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE
Sbjct: 301  ALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLE 360

Query: 361  EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPN 420
            EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNI+KTVNQIEAVF +TG+E EAVSTPN
Sbjct: 361  EQIKKWDADKIRKFHERWYFPANATLYIVGDIDNISKTVNQIEAVFDDTGLEKEAVSTPN 420

Query: 421  PSAFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVD 480
            PSAFGAMASFLVPKISVGL GSLSNERSNSVDQSKIIKKERH IRPPVKHNWSLPGSNV+
Sbjct: 421  PSAFGAMASFLVPKISVGLGGSLSNERSNSVDQSKIIKKERHAIRPPVKHNWSLPGSNVN 480

Query: 481  TNPPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNP 540
             NPPQIFQHELLQNFSI MFCK+PVNKV+TFSDLRNVLMKRIFLSALHFRINTRYKSSNP
Sbjct: 481  ANPPQIFQHELLQNFSIYMFCKVPVNKVQTFSDLRNVLMKRIFLSALHFRINTRYKSSNP 540

Query: 541  PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD 600
            PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD
Sbjct: 541  PFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMD 600

Query: 601  ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI 660
            ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI
Sbjct: 601  ALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSI 660

Query: 661  GAEVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIE 720
            GAEVLEFISDYGKPT+PLPAAIVACVPKKAHIDGLGETEFK+TASEIITAIEAGL+EPIE
Sbjct: 661  GAEVLEFISDYGKPTSPLPAAIVACVPKKAHIDGLGETEFKLTASEIITAIEAGLEEPIE 720

Query: 721  AEPELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNY 780
            AEPELEVPKELISSSQIAELRMQ KPSFIP NPETSVTKFHDKETGITQCRLSNGIPVNY
Sbjct: 721  AEPELEVPKELISSSQIAELRMQHKPSFIPSNPETSVTKFHDKETGITQCRLSNGIPVNY 780

Query: 781  KISKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL 840
            KISKSENK+GVMRLIVGGGRAAESPD QGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL
Sbjct: 781  KISKSENKSGVMRLIVGGGRAAESPDLQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHL 840

Query: 841  INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQ 900
            INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE         HSVWLEDAFDRAKQ
Sbjct: 841  INCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE---------HSVWLEDAFDRAKQ 900

Query: 901  LYMSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNN 960
            LYMSYY SIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVK+AVMNQFVGNN
Sbjct: 901  LYMSYYWSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKDAVMNQFVGNN 960

Query: 961  MEVSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTD 1020
            MEVS+VGDFSEEE+ESCILDYLGTVTA T  E AP SVPIVFRPSPSELQFQQVFLKDTD
Sbjct: 961  MEVSVVGDFSEEEIESCILDYLGTVTAKTPCETAPASVPIVFRPSPSELQFQQVFLKDTD 1020

Query: 1021 ERACAYISGPAPNRWGVTVDGLELLETVSHISRTDVNDAFDNDIEKGLQRKLRSHPLFFG 1080
            ERACAYISGPAPNRWGVTVDG+ELLE+VS ISRTD +D  DNDIEKGLQRKLRSHPLFFG
Sbjct: 1021 ERACAYISGPAPNRWGVTVDGVELLESVSQISRTDESDESDNDIEKGLQRKLRSHPLFFG 1080

Query: 1081 ITMGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTST 1140
            ITMGLLAEIINSR          LFT+VRDSLGLTYDVSFE+SLFDRLKLGWYVISVTST
Sbjct: 1081 ITMGLLAEIINSR----------LFTNVRDSLGLTYDVSFELSLFDRLKLGWYVISVTST 1140

Query: 1141 PGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS 1200
            P KVYKAVDACKNVLRGLH NKI+QRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS
Sbjct: 1141 PAKVYKAVDACKNVLRGLHGNKISQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASS 1200

Query: 1201 VPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEEE 1260
            VPRKDLSCIKDLTSLYEAATIDD+YIAYDQLKVDADSLYTCIG+AGAQAGEE+I SFEEE
Sbjct: 1201 VPRKDLSCIKDLTSLYEAATIDDVYIAYDQLKVDADSLYTCIGVAGAQAGEESIVSFEEE 1260

Query: 1261 GSDQDFQGVVPTGRGLSTMTRPTT 1282
            GSDQDFQGVVPTGRGLSTMTRPTT
Sbjct: 1261 GSDQDFQGVVPTGRGLSTMTRPTT 1265

BLAST of MS022004 vs. ExPASy TrEMBL
Match: A0A1S3B556 (stromal processing peptidase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485911 PE=4 SV=1)

HSP 1 Score: 2283.1 bits (5915), Expect = 0.0e+00
Identity = 1176/1282 (91.73%), Postives = 1209/1282 (94.31%), Query Frame = 0

Query: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRS-CTPLARFNVDSRFVVSLRR 60
            MAVA+SSTVSNLT RRPLLSLKD   P +R+NS++LP RS C  L+RF+V+SRFVV LRR
Sbjct: 1    MAVATSSTVSNLTHRRPLLSLKDQTTPIKRVNSVQLPSRSICAHLSRFDVESRFVVPLRR 60

Query: 61   HSHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRFTPR 120
            +S +DGIGR+KFRRNKDNARRP A KI ERGNET    NCISC LNQKR   SIKR T R
Sbjct: 61   YSREDGIGRHKFRRNKDNARRPCAYKIGERGNET--LTNCISCFLNQKRRCPSIKRPTSR 120

Query: 121  FIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPEFG 180
            FI DKSA +LSKNE D KVVKHA IVC TVGPDEPHAA TAWPDGILEKQDLD+SYPEFG
Sbjct: 121  FILDKSAFQLSKNERDAKVVKHARIVCGTVGPDEPHAAPTAWPDGILEKQDLDVSYPEFG 180

Query: 181  RAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG 240
            RAELEAFLSSELPSHPKLYRGQLKNGL+YLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG
Sbjct: 181  RAELEAFLSSELPSHPKLYRGQLKNGLKYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG 240

Query: 241  IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDAL 300
            IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDAL
Sbjct: 241  IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDAL 300

Query: 301  NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQ 360
            NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLS+RFPIGLEEQ
Sbjct: 301  NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQ 360

Query: 361  IKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPS 420
            IKKWDADKIRKFHERWYFPANATLYIVGDIDNI+K VNQIEAVF ETG+ENEAVSTPNPS
Sbjct: 361  IKKWDADKIRKFHERWYFPANATLYIVGDIDNISKAVNQIEAVFGETGLENEAVSTPNPS 420

Query: 421  AFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVDTN 480
            AFGAMASFLVPKISVGL GSLSNERSNSVDQSKIIKKERH IRPPVKH WSLPGSNVD N
Sbjct: 421  AFGAMASFLVPKISVGLGGSLSNERSNSVDQSKIIKKERHAIRPPVKHYWSLPGSNVDAN 480

Query: 481  PPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF 540
            PPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF
Sbjct: 481  PPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF 540

Query: 541  TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL 600
            TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL
Sbjct: 541  TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL 600

Query: 601  LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA 660
            LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA
Sbjct: 601  LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA 660

Query: 661  EVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAE 720
            EVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGL EPIEAE
Sbjct: 661  EVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLREPIEAE 720

Query: 721  PELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKI 780
            PELEVPKELISSSQI ELRMQ +PSF+PLNPET+VTKFHDKETGITQCRLSNGIPVNYKI
Sbjct: 721  PELEVPKELISSSQIVELRMQHQPSFVPLNPETNVTKFHDKETGITQCRLSNGIPVNYKI 780

Query: 781  SKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLIN 840
            SKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVG+FSREQVELFCVNHLIN
Sbjct: 781  SKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGTFSREQVELFCVNHLIN 840

Query: 841  CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQLY 900
            CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE         HSVWLEDAFDRAKQLY
Sbjct: 841  CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE---------HSVWLEDAFDRAKQLY 900

Query: 901  MSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNME 960
            MSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVK+AVMNQFVGNNME
Sbjct: 901  MSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKDAVMNQFVGNNME 960

Query: 961  VSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTDER 1020
            VSLVGDFSEEE+ESCILDYLGTVTAT  SE A  SVPIVFRPS SELQFQQVFLKDTDER
Sbjct: 961  VSLVGDFSEEEIESCILDYLGTVTATKTSEAALASVPIVFRPSASELQFQQVFLKDTDER 1020

Query: 1021 ACAYISGPAPNRWGVTVDGLELLETVSHISRTDVNDAFDNDIEKGLQRKLRSHPLFFGIT 1080
            ACAYISGPAPNRWGVT +GLELLE+VS ISRT  +D  D+DIEKGLQRKLRSHPLFFGIT
Sbjct: 1021 ACAYISGPAPNRWGVTFEGLELLESVSQISRTGESDESDSDIEKGLQRKLRSHPLFFGIT 1080

Query: 1081 MGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTSTPG 1140
            MGLLAEIINSR          LFTSVRDSLGLTYDVSFE+SLFDRLKLGWYVISVTSTP 
Sbjct: 1081 MGLLAEIINSR----------LFTSVRDSLGLTYDVSFELSLFDRLKLGWYVISVTSTPA 1140

Query: 1141 KVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP 1200
            KVYKAVDACK+VLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP
Sbjct: 1141 KVYKAVDACKSVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP 1200

Query: 1201 RKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEEEGS 1260
            RKDLSCIKDLTSLYEAATIDD+YIAYDQLKVDADSLYTCIGIAGAQAGEE+I SFEEEGS
Sbjct: 1201 RKDLSCIKDLTSLYEAATIDDVYIAYDQLKVDADSLYTCIGIAGAQAGEESIVSFEEEGS 1260

Query: 1261 DQDFQGVVPTGRGLSTMTRPTT 1282
            DQDFQGV+P+GRGLSTMTRPTT
Sbjct: 1261 DQDFQGVIPSGRGLSTMTRPTT 1261

BLAST of MS022004 vs. ExPASy TrEMBL
Match: A0A0A0LH02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G072490 PE=4 SV=1)

HSP 1 Score: 2282.3 bits (5913), Expect = 0.0e+00
Identity = 1175/1282 (91.65%), Postives = 1209/1282 (94.31%), Query Frame = 0

Query: 1    MAVASSSTVSNLTQRRPLLSLKDSGAPNRRLNSLRLPPRSCTP-LARFNVDSRFVVSLRR 60
            MAVA+SSTVSNLTQRRPLLSLKD   P +R+NS++LP RS +  L+RF+VDSRFVV LRR
Sbjct: 1    MAVATSSTVSNLTQRRPLLSLKDQTTPIKRVNSVQLPSRSISAHLSRFDVDSRFVVPLRR 60

Query: 61   HSHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKRFTPR 120
            HS DDGIGR+KFRRNKDNARRP A KI E GNET    NCISC LNQKR   SIKR T R
Sbjct: 61   HSRDDGIGRHKFRRNKDNARRPCAYKIGEHGNET--LTNCISCFLNQKRRCPSIKRPTSR 120

Query: 121  FIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGILEKQDLDISYPEFG 180
            FI DKSA +LSKNE DD+VVKHA IVC TVGPDEPHAA TAWPDGILEKQDLD+SYPEFG
Sbjct: 121  FILDKSAFQLSKNERDDRVVKHARIVCGTVGPDEPHAAPTAWPDGILEKQDLDVSYPEFG 180

Query: 181  RAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG 240
            RAELEAFLSSELPSHPKLYRGQLKNGL+YLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG
Sbjct: 181  RAELEAFLSSELPSHPKLYRGQLKNGLKYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQG 240

Query: 241  IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDAL 300
            IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDAL
Sbjct: 241  IAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDAL 300

Query: 301  NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPIGLEEQ 360
            NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLS+RFPIGLEEQ
Sbjct: 301  NEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQ 360

Query: 361  IKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAVSTPNPS 420
            IKKWDADKIRKFHERWYFPANATLYIVGDIDNI+K VNQIEAVF E+G+ENEAVSTPNPS
Sbjct: 361  IKKWDADKIRKFHERWYFPANATLYIVGDIDNISKAVNQIEAVFGESGLENEAVSTPNPS 420

Query: 421  AFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPGSNVDTN 480
            AFGAMASFLVPKISVGL GSLSNERSNSVDQSKI+KKERH IRPPV HNWSLPGSNV  N
Sbjct: 421  AFGAMASFLVPKISVGLGGSLSNERSNSVDQSKIVKKERHAIRPPVMHNWSLPGSNVHAN 480

Query: 481  PPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF 540
            PPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF
Sbjct: 481  PPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYKSSNPPF 540

Query: 541  TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL 600
            TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL
Sbjct: 541  TSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELTRYMDAL 600

Query: 601  LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA 660
            LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA
Sbjct: 601  LKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEEVNSIGA 660

Query: 661  EVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLDEPIEAE 720
            EVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEI TAIEAGL EPIEAE
Sbjct: 661  EVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEITTAIEAGLREPIEAE 720

Query: 721  PELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGIPVNYKI 780
            PELEVPKELISSSQIAELR+Q +PSFI LNPET+VTKFHDKETGITQCRLSNGIPVNYKI
Sbjct: 721  PELEVPKELISSSQIAELRIQHQPSFIRLNPETNVTKFHDKETGITQCRLSNGIPVNYKI 780

Query: 781  SKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLIN 840
            SKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVG+FSREQVELFCVNHLIN
Sbjct: 781  SKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGTFSREQVELFCVNHLIN 840

Query: 841  CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFDRAKQLY 900
            CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE         HSVWLEDAFDRAKQLY
Sbjct: 841  CSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLE---------HSVWLEDAFDRAKQLY 900

Query: 901  MSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQFVGNNME 960
            MSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVK+AVMNQFVGNNME
Sbjct: 901  MSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKDAVMNQFVGNNME 960

Query: 961  VSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFLKDTDER 1020
            VSLVGDFSEEE+ESCILDYLGTVTATT SE A  SVPIVFRPS SELQFQQVFLKDTDER
Sbjct: 961  VSLVGDFSEEEIESCILDYLGTVTATTTSEAALASVPIVFRPSASELQFQQVFLKDTDER 1020

Query: 1021 ACAYISGPAPNRWGVTVDGLELLETVSHISRTDVNDAFDNDIEKGLQRKLRSHPLFFGIT 1080
            ACAYISGPAPNRWGVT +GLELLE++S ISRT  +D  DNDIEKGLQRKLRSHPLFFGIT
Sbjct: 1021 ACAYISGPAPNRWGVTFEGLELLESISQISRTGESDESDNDIEKGLQRKLRSHPLFFGIT 1080

Query: 1081 MGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLGWYVISVTSTPG 1140
            MGLLAEIINSR          LFTSVRDSLGLTYDVSFE+SLFDRLKLGWYVISVTSTP 
Sbjct: 1081 MGLLAEIINSR----------LFTSVRDSLGLTYDVSFELSLFDRLKLGWYVISVTSTPA 1140

Query: 1141 KVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP 1200
            KVYKAVDACK+VLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP
Sbjct: 1141 KVYKAVDACKSVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVP 1200

Query: 1201 RKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGEENIGSFEEEGS 1260
            RKDLSCIKDLTSLYEAATIDD+YIAYDQLKVDADSLYTCIGIAGAQAGEE+I SFEEEGS
Sbjct: 1201 RKDLSCIKDLTSLYEAATIDDVYIAYDQLKVDADSLYTCIGIAGAQAGEESIVSFEEEGS 1260

Query: 1261 DQDFQGVVPTGRGLSTMTRPTT 1282
            DQDFQGV+P+GRGLSTMTRPTT
Sbjct: 1261 DQDFQGVIPSGRGLSTMTRPTT 1261

BLAST of MS022004 vs. TAIR 10
Match: AT5G42390.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 1813.5 bits (4696), Expect = 0.0e+00
Identity = 944/1293 (73.01%), Postives = 1081/1293 (83.60%), Query Frame = 0

Query: 4    ASSSTVSNLTQRRPLLSLKDSGAPNR------RLNSLRLPPRS--CTPLARFNVDSRFVV 63
            +SSS++    +  P+L+  +SG   R        N +R  P S   TP  R  V++  ++
Sbjct: 3    SSSSSIFTGVKFSPILAPFNSGDSRRSRYLKDSRNKVRFNPSSPRLTP-HRVRVEAPSLI 62

Query: 64   SLRRHSHDDGIGRYKFRRNKDNARRPGASKIVERGNETSGTRNCISCCLNQKRGRSSIKR 123
                    +G+   +   +K   +R   S     G   S  RN    CL  KR ++ I+R
Sbjct: 63   PY------NGLWAAQPNSHKGRLKRNIVSGKEATGISLSQGRN---FCLTCKRNQAGIRR 122

Query: 124  FTPRFIFDKSALRLSKNEHDDKVVKHAPIVCATVGPDEPHAANTAWPDGIL-EKQDLDIS 183
              P    D++A  LS++     + KH+ IV AT+GPDEPHAA TAWPDGI+ E+QDLD+ 
Sbjct: 123  ALPSAFVDRTAFSLSRSSLTSSLRKHSQIVNATLGPDEPHAAGTAWPDGIVAERQDLDLL 182

Query: 184  YPEFGRAELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEE 243
             PE   AELEAFL  ELPSHPKL+RGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEE
Sbjct: 183  PPEIDSAELEAFLGCELPSHPKLHRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEE 242

Query: 244  DDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPS 303
            +DEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPT TKDS+ DL PS
Sbjct: 243  EDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTHTKDSEDDLFPS 302

Query: 304  VLDALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRRFPI 363
            VLDALNEIAFHPKFL+SRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKL RRFPI
Sbjct: 303  VLDALNEIAFHPKFLSSRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLGRRFPI 362

Query: 364  GLEEQIKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENEAV- 423
            GLEEQIKKWD DKIRKFHERWYFPANATLYIVGDIDNI + V+ IEAVF + G++NE+  
Sbjct: 363  GLEEQIKKWDVDKIRKFHERWYFPANATLYIVGDIDNIPRIVHNIEAVFGKNGLDNESTP 422

Query: 424  STPNPSAFGAMASFLVPKISVGLTGSLSNERSNSVDQSKIIKKERHVIRPPVKHNWSLPG 483
            S+P+P AFGAMA+FLVPK+  GL G+ SNE++N+ DQSK+IK+ERH IRPPV+HNWSLPG
Sbjct: 423  SSPSPGAFGAMANFLVPKLPAGLGGTFSNEKTNTADQSKMIKRERHAIRPPVEHNWSLPG 482

Query: 484  SNVDTNPPQIFQHELLQNFSINMFCKIPVNKVRTFSDLRNVLMKRIFLSALHFRINTRYK 543
            ++VD  PPQIF+HELLQNF+INMFCKIPV+KV+TF DLRNVLMKRIFLSALHFRINTRYK
Sbjct: 483  TSVDLKPPQIFKHELLQNFAINMFCKIPVSKVQTFGDLRNVLMKRIFLSALHFRINTRYK 542

Query: 544  SSNPPFTSIELDHSDSGREGCTVTTLTVTAEPKNWQSAIKVAVQEVRRLKEFGVTKGELT 603
            SSNPPFTS+ELDHSDSGREGCTVTTLTVTAEP+NWQ+A+KVAVQEVRRLKEFGVT+GELT
Sbjct: 543  SSNPPFTSVELDHSDSGREGCTVTTLTVTAEPQNWQNAVKVAVQEVRRLKEFGVTRGELT 602

Query: 604  RYMDALLKDSEHLAAMIDNVSSVDNLDFIMESDALGHTVMDQRQGHESLVAVAGTVTLEE 663
            RYMDALLKDSEHLAAMIDNVSSVDNLDFIMESDAL HTVMDQ QGHE+LVAVAGTVTLEE
Sbjct: 603  RYMDALLKDSEHLAAMIDNVSSVDNLDFIMESDALSHTVMDQTQGHETLVAVAGTVTLEE 662

Query: 664  VNSIGAEVLEFISDYGKPTAPLPAAIVACVPKKAHIDGLGETEFKITASEIITAIEAGLD 723
            VN++GA+VLEFISD+G+PTAPLPAAIVACVP K H+DG+GE++F I+  EII ++++GL 
Sbjct: 663  VNTVGAKVLEFISDFGRPTAPLPAAIVACVPTKVHVDGVGESDFNISPDEIIESVKSGLL 722

Query: 724  EPIEAEPELEVPKELISSSQIAELRMQQKPSFIPLNPETSVTKFHDKETGITQCRLSNGI 783
             PIEAEPELEVPKELIS SQ+ EL +Q+ P F+P+ P + +TK HDKETGITQ RLSNGI
Sbjct: 723  APIEAEPELEVPKELISQSQLKELTLQRNPCFVPI-PGSGLTKLHDKETGITQLRLSNGI 782

Query: 784  PVNYKISKSENKAGVMRLIVGGGRAAESPDSQGAVVVGVRTLSEGGRVGSFSREQVELFC 843
             VNYK S +E++AGVMRLIVGGGRAAE+ DS+GAVVVGVRTLSEGGRVG FSREQVELFC
Sbjct: 783  AVNYKKSTTESRAGVMRLIVGGGRAAETSDSKGAVVVGVRTLSEGGRVGDFSREQVELFC 842

Query: 844  VNHLINCSLESTEEFIAMEFRFTLRDNGMRAAFQLLHMVLEAFMVITYLQHSVWLEDAFD 903
            VNHLINCSLESTEEFIAMEFRFTLRDNGM+AAFQLLHMVLE          SVWLEDAFD
Sbjct: 843  VNHLINCSLESTEEFIAMEFRFTLRDNGMQAAFQLLHMVLE---------RSVWLEDAFD 902

Query: 904  RAKQLYMSYYRSIPKSLERSTAHKLMLAMLNGDERFVEPSPKSLQNLTLQTVKNAVMNQF 963
            RA+QLY+SY+RSIPKSLER+TAHKLM+AMLNGDERFVEP+PKSLQ+L L++VK+AVM+ F
Sbjct: 903  RARQLYLSYFRSIPKSLERATAHKLMIAMLNGDERFVEPTPKSLQSLNLESVKDAVMSHF 962

Query: 964  VGNNMEVSLVGDFSEEEVESCILDYLGTVTATTNSERAPPSVPIVFRPSPSELQFQQVFL 1023
            VG+NMEVS+VGDFSEEE+E CILDYLGTV A+ +S + P S PI+FR   + LQFQQVFL
Sbjct: 963  VGDNMEVSIVGDFSEEEIERCILDYLGTVKASHDSAKPPGSEPILFRQPTAGLQFQQVFL 1022

Query: 1024 KDTDERACAYISGPAPNRWGVTVDGLELLETVSH--ISRTDVNDAFDNDIEKG---LQRK 1083
            KDTDERACAYI+GPAPNRWG TVDG +L ++VS   ++   +  + +  +E G   LQ+K
Sbjct: 1023 KDTDERACAYIAGPAPNRWGFTVDGDDLFQSVSKLPVAHDGLLKSEEQLLEGGDRELQKK 1082

Query: 1084 LRSHPLFFGITMGLLAEIINSRQVFSANCIFLLFTSVRDSLGLTYDVSFEVSLFDRLKLG 1143
            LR+HPLFFG+TMGLLAEIINSR          LFT+VRDSLGLTYDVSFE++LFDRL LG
Sbjct: 1083 LRAHPLFFGVTMGLLAEIINSR----------LFTTVRDSLGLTYDVSFELNLFDRLNLG 1142

Query: 1144 WYVISVTSTPGKVYKAVDACKNVLRGLHSNKIAQRELDRAKRTLLMRHEAEIKSNAYWLG 1203
            WYVISVTSTPGKVYKAVDACK+VLRGLHSN+IA RELDRAKRTLLMRHEAE+KSNAYWL 
Sbjct: 1143 WYVISVTSTPGKVYKAVDACKSVLRGLHSNQIAPRELDRAKRTLLMRHEAELKSNAYWLN 1202

Query: 1204 LLAHLQASSVPRKDLSCIKDLTSLYEAATIDDIYIAYDQLKVDADSLYTCIGIAGAQAGE 1263
            LLAHLQASSVPRK+LSCIK+L SLYEAA+I+DIY+AY+QL+VD DSLY+CIGIAGAQAGE
Sbjct: 1203 LLAHLQASSVPRKELSCIKELVSLYEAASIEDIYLAYNQLRVDEDSLYSCIGIAGAQAGE 1262

Query: 1264 ENIGSFEEEGSDQDFQGVVPTGRGLSTMTRPTT 1282
            E     EEE  +  F GVVP GRG S  TRPTT
Sbjct: 1263 EITVLSEEEEPEDVFSGVVPVGRGSSMTTRPTT 1265

BLAST of MS022004 vs. TAIR 10
Match: AT5G56730.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 98.6 bits (244), Expect = 3.9e-20
Identity = 69/245 (28.16%), Postives = 115/245 (46.94%), Query Frame = 0

Query: 182 ELEAFLSSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGIA 241
           ++E  L +EL      Y G+L NGL Y +  N  P  R    + V VGS+ EE+D++G+A
Sbjct: 26  DMEQELGNELEPFGADY-GRLDNGLIYYVRRNSKPRMRAALALAVKVGSVLEEEDQRGVA 85

Query: 242 HMIEHVAFLGSKKRE-----KLLGT-----GARSNAYTDFHHTVFHIHSPTSTKDSDGDL 301
           H++EH+AF  + +       K L +     G   NA T    T++ +  P        +L
Sbjct: 86  HIVEHLAFSATTRYTNHDIVKFLESIGAEFGPCQNAMTTADETIYELFVPVDKP----EL 145

Query: 302 LPSVLDALNEIAFHPKFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSRR 361
           L   +  L E +   +     +EKER A++ E +       R+     Q +   +K + R
Sbjct: 146 LSQAISILAEFSSEIRVSKEDLEKERGAVMEEYRGNRNATGRMQDSHWQLMMEGSKYAER 205

Query: 362 FPIGLEEQIKKWDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSETGVENE 417
            PIGLE+ I+   A  +++F+++WY   N  +  VGD  +    V+ I+  F +    +E
Sbjct: 206 LPIGLEKVIRSVPAATVKQFYQKWYHLCNMAVVAVGDFPDTKTVVDLIKTHFEDKRSSSE 265

BLAST of MS022004 vs. TAIR 10
Match: AT3G02090.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 63.2 bits (152), Expect = 1.8e-09
Identity = 56/223 (25.11%), Postives = 100/223 (44.84%), Query Frame = 0

Query: 193 SHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLGS 252
           S P+     L NGLR +   + +        + +  GS  E D+  G AH +EH+ F G+
Sbjct: 93  SAPETRVTTLPNGLR-VATESNLSAKTATVGVWIDAGSRFESDETNGTAHFLEHMIFKGT 152

Query: 253 KKR------EKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDALNEIAFHP 312
            +R      E++   G   NAYT    T ++        DS+   +   LD L +I  + 
Sbjct: 153 DRRTVRALEEEIEDIGGHLNAYTSREQTTYY----AKVLDSN---VNQALDVLADILQNS 212

Query: 313 KFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSE----NKLSRRFPIGLEEQIKK 372
           KF   R+ +ER  IL E+Q    +E + D  +L HLH+       L R   +G  + +K 
Sbjct: 213 KFEEQRINRERDVILREMQ---EVEGQTDEVVLDHLHATAFQYTPLGRTI-LGPAQNVKS 272

Query: 373 WDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSE 406
              + ++ + +  Y  +   +   G + +  + V Q++ +F++
Sbjct: 273 ITREDLQNYIKTHYTASRMVIAAAGAVKH-EEVVEQVKKLFTK 302

BLAST of MS022004 vs. TAIR 10
Match: AT3G02090.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 63.2 bits (152), Expect = 1.8e-09
Identity = 56/223 (25.11%), Postives = 100/223 (44.84%), Query Frame = 0

Query: 193 SHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLGS 252
           S P+     L NGLR +   + +        + +  GS  E D+  G AH +EH+ F G+
Sbjct: 93  SAPETRVTTLPNGLR-VATESNLSAKTATVGVWIDAGSRFESDETNGTAHFLEHMIFKGT 152

Query: 253 KKR------EKLLGTGARSNAYTDFHHTVFHIHSPTSTKDSDGDLLPSVLDALNEIAFHP 312
            +R      E++   G   NAYT    T ++        DS+   +   LD L +I  + 
Sbjct: 153 DRRTVRALEEEIEDIGGHLNAYTSREQTTYY----AKVLDSN---VNQALDVLADILQNS 212

Query: 313 KFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSE----NKLSRRFPIGLEEQIKK 372
           KF   R+ +ER  IL E+Q    +E + D  +L HLH+       L R   +G  + +K 
Sbjct: 213 KFEEQRINRERDVILREMQ---EVEGQTDEVVLDHLHATAFQYTPLGRTI-LGPAQNVKS 272

Query: 373 WDADKIRKFHERWYFPANATLYIVGDIDNIAKTVNQIEAVFSE 406
              + ++ + +  Y  +   +   G + +  + V Q++ +F++
Sbjct: 273 ITREDLQNYIKTHYTASRMVIAAAGAVKH-EEVVEQVKKLFTK 302

BLAST of MS022004 vs. TAIR 10
Match: AT1G06900.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.3e-04
Identity = 32/109 (29.36%), Postives = 49/109 (44.95%), Query Frame = 0

Query: 222 AHMEVHVGSIDEEDDEQGIAHMIEHVAFLGSKK-------REKLLGTGARSNAYTDFHHT 281
           A M V +GS  +  + QG+AH +EH+ F+GS +          L   G  SNAYT+  HT
Sbjct: 109 AAMCVSMGSFLDPPEAQGLAHFLEHMLFMGSTEFPDENEYDSYLSKHGGSSNAYTEMEHT 168

Query: 282 VFHIHSPTSTKDSDGDLLPSVLDALNEIAFHPKFLASRVEKERRAILSE 324
            +H        +   + L   L   ++    P      +E+E  A+ SE
Sbjct: 169 CYHF-------EVKREFLQGALKRFSQFFVAPLMKTEAMEREVLAVDSE 210