MS021966 (gene) Bitter gourd (TR) v1

BLAST of MS021966 vs. NCBI nr
Match: XP_022149925.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 2613.2 bits (6772), Expect = 0.0e+00
Identity = 1334/1346 (99.11%), Postives = 1338/1346 (99.41%), Query Frame = 0

Query: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
            MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD
Sbjct: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60

Query: 61   SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
            SAKSNGDEIEGMVVEWLS AEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE
Sbjct: 61   SAKSNGDEIEGMVVEWLSIAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120

Query: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180
            VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG
Sbjct: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180

Query: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240
            MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR
Sbjct: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240

Query: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300
            ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN
Sbjct: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300

Query: 301  IKINVLSKKEARNFFNKMACDFVDYNDSDPEMEAVATELADECAGLPLALATVAQALKGK 360
            IKINVLSKKEARNFFNKMACDFVD NDSDPEMEAVATELADECAGLPLALATVAQALKGK
Sbjct: 301  IKINVLSKKEARNFFNKMACDFVDCNDSDPEMEAVATELADECAGLPLALATVAQALKGK 360

Query: 361  GRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQIN 420
            GRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQIN
Sbjct: 361  GRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQIN 420

Query: 421  IKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTAI 480
            IKYLLMYAMGLGLLNGMSSL+VARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTAI
Sbjct: 421  IKYLLMYAMGLGLLNGMSSLVVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTAI 480

Query: 481  LVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGKR 540
            LVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGKR
Sbjct: 481  LVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGKR 540

Query: 541  TPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELKK 600
            TPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDM IVGELKK
Sbjct: 541  TPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMXIVGELKK 600

Query: 601  LEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFKL 660
            LEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFKL
Sbjct: 601  LEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFKL 660

Query: 661  WNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDWS 720
            WNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDWS
Sbjct: 661  WNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDWS 720

Query: 721  GKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDELRSAKNILFSLDHKGFPKLKRLR 780
            GKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDELRSAKNILFSLDHKGFPKLKRLR
Sbjct: 721  GKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDELRSAKNILFSLDHKGFPKLKRLR 780

Query: 781  VHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVKVES 840
            VHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVKVES
Sbjct: 781  VHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVKVES 840

Query: 841  CSRLKFVFPPSMGGGLVLQKLEISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLP 900
            CSRLKFVFPPSMGGGLVLQKLEISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLP
Sbjct: 841  CSRLKFVFPPSMGGGLVLQKLEISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLP 900

Query: 901  ELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETLKLHALNSGKIWQ 960
            ELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETLKLHALNSGKIWQ
Sbjct: 901  ELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETLKLHALNSGKIWQ 960

Query: 961  DQLPASFSGVKNLTSLSVEGCASIKYLMTITMAKSLVHLERLEISNCKLMKDITISEARD 1020
            DQLPASFSGVKNLTSLSVEGCASIKYL TITMAKSLVHLERLEISNCKLMKDITISEARD
Sbjct: 961  DQLPASFSGVKNLTSLSVEGCASIKYLTTITMAKSLVHLERLEISNCKLMKDITISEARD 1020

Query: 1021 LGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLKKVDIRHCRRLET 1080
            LGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLKKVDIRHCRRLET
Sbjct: 1021 LGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLKKVDIRHCRRLET 1080

Query: 1081 IFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHLKELKLLRLPKLK 1140
            IFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHLKELKLLRLPKLK
Sbjct: 1081 IFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHLKELKLLRLPKLK 1140

Query: 1141 HIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQFCGVEEIVSKGG 1200
            HIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQFCGVEEIVSKGG
Sbjct: 1141 HIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQFCGVEEIVSKGG 1200

Query: 1201 GEDEDAAFVLTGLISLHLWNLFEFKRFYPGKYTLDCPSLQLLDVRHCKSFKLLEGALEKL 1260
            GEDED AF+LTGL SLHLWNLFEFKRFYPGKYTLDCPSLQLLDVRHCKSFKLLEGALEK 
Sbjct: 1201 GEDEDVAFLLTGLTSLHLWNLFEFKRFYPGKYTLDCPSLQLLDVRHCKSFKLLEGALEKS 1260

Query: 1261 SPPADDKVEDDQSSKKEQLERRESDVLHEMSTSKKEERTTTTAQEDKVEAELTEIRAQLQ 1320
            SPPAD+KVEDDQSSKKEQLERRESDVL+EMSTSKKEERTTTTAQED VEAELTEIRAQLQ
Sbjct: 1261 SPPADEKVEDDQSSKKEQLERRESDVLNEMSTSKKEERTTTTAQEDTVEAELTEIRAQLQ 1320

Query: 1321 TLVMGQNQMMEHLAQLTAIARQTASR 1347
            TLVMGQNQMMEHLAQLTAIARQTASR
Sbjct: 1321 TLVMGQNQMMEHLAQLTAIARQTASR 1346

BLAST of MS021966 vs. NCBI nr
Match: XP_023546944.1 (disease resistance protein RPS2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2137.5 bits (5537), Expect = 0.0e+00
Identity = 1104/1364 (80.94%), Postives = 1215/1364 (89.08%), Query Frame = 0

Query: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
            MEICS AIVGPIGE+ITNC VDPVFRQLDYL+HFRSNV NL DQ KKLVETRDFVQH+VD
Sbjct: 1    MEICSAAIVGPIGEKITNCTVDPVFRQLDYLVHFRSNVNNLSDQRKKLVETRDFVQHAVD 60

Query: 61   SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
            SAKSNGDEIEGMVVEWLS  +K+S++VDRFFNEANGRSLRWWNM SRHRFSRR+ K+A+E
Sbjct: 61   SAKSNGDEIEGMVVEWLSITKKYSDEVDRFFNEANGRSLRWWNMLSRHRFSRRSTKMAME 120

Query: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180
            VVKAIEEGRFERVAYR+PP GI  + NSKKFEAFESRV I KEIIEALADA ASVIVVHG
Sbjct: 121  VVKAIEEGRFERVAYRIPPQGIMMLNNSKKFEAFESRVLILKEIIEALADANASVIVVHG 180

Query: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240
            MGGVGKTTL EE+ARL IE KLFDA+AM TVTQ P+VKRIQGEIADQLGLKFEEE +RVR
Sbjct: 181  MGGVGKTTLVEEIARLVIEGKLFDALAMTTVTQIPNVKRIQGEIADQLGLKFEEEKDRVR 240

Query: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300
            A+RLRRRLEMEKKVLVILDDVWAKLDLE +G S HHKGCKILVTSRKDDL+F DFGTQKN
Sbjct: 241  ADRLRRRLEMEKKVLVILDDVWAKLDLEDVGISSHHKGCKILVTSRKDDLYFGDFGTQKN 300

Query: 301  IKINVLSKKEARNFFNKMACDFVD-YNDSDPEMEAVATELADECAGLPLALATVAQALKG 360
            IKI+VL+K EARNFFNKMACDFV+  NDSDPEMEAVATELADECAGLPLALATVAQALKG
Sbjct: 301  IKIDVLAKNEARNFFNKMACDFVESSNDSDPEMEAVATELADECAGLPLALATVAQALKG 360

Query: 361  KGRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQI 420
            KGRPIWNDAL ELKFP QPNNYGVNKE YSSLKLSYKSL+RDEARSLFLLCSLFP+DYQI
Sbjct: 361  KGRPIWNDALHELKFPSQPNNYGVNKEAYSSLKLSYKSLNRDEARSLFLLCSLFPQDYQI 420

Query: 421  NIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA 480
            NIKYLLMYAMGLGLLNGM+SL  A+ RILSLVDELKTSYLLLDG DSDFVKMHDIVRDTA
Sbjct: 421  NIKYLLMYAMGLGLLNGMNSLAAAKWRILSLVDELKTSYLLLDGVDSDFVKMHDIVRDTA 480

Query: 481  ILVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGK 540
            IL+ASKMKSKYLVRHG GE +WPP+DEFKDYTAISLGCSD +ELPEF CPQL+FLLLVGK
Sbjct: 481  ILIASKMKSKYLVRHGAGESLWPPMDEFKDYTAISLGCSDHSELPEFICPQLRFLLLVGK 540

Query: 541  RTPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELK 600
            RT LRLPE FFAGMQELRVLDLT LCI QLPSSI+QLVNL TLCLDDCVLPDMSIVGELK
Sbjct: 541  RTSLRLPEKFFAGMQELRVLDLTSLCIQQLPSSIDQLVNLHTLCLDDCVLPDMSIVGELK 600

Query: 601  KLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFK 660
            KLEILSLRASDI+ALPRVIG+LTNLK+LNLSNC KLKVIPANLLS+LTGL ELY+DNSFK
Sbjct: 601  KLEILSLRASDIIALPRVIGELTNLKVLNLSNCFKLKVIPANLLSKLTGLSELYMDNSFK 660

Query: 661  LWNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDW 720
             WNVGH NEGNINA I+ELN LPRLTTLHVHI +  +LP AS+F NL GYRILIGDGWDW
Sbjct: 661  HWNVGHMNEGNINARISELNYLPRLTTLHVHIPNPTLLPNASVFGNLSGYRILIGDGWDW 720

Query: 721  SGKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDEL--RSAKNILFSLDHKGFPKLK 780
            SGK+ETSKTLKLKLE   QREDAIQALLENTE+LYLDEL   SA++IL+SLDHKGFPKLK
Sbjct: 721  SGKFETSKTLKLKLERGIQREDAIQALLENTENLYLDELGSASARDILYSLDHKGFPKLK 780

Query: 781  RLRVHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVK 840
             LRV NN+EIV+VVNS+ MQH HSAFPLLESL LKNL  LG ICRG LPQMSFRNLK VK
Sbjct: 781  CLRVKNNSEIVSVVNSDGMQHLHSAFPLLESLFLKNLDELGSICRGRLPQMSFRNLKIVK 840

Query: 841  VESCSRLKFVFPPSMGGGLV-LQKLEISECPVMETVVS--------IHDDKMDEKNMIEF 900
            VESC+RLKFVFP  MG GL+ LQKLEISEC  MET+VS        I+ DK DEKN IEF
Sbjct: 841  VESCNRLKFVFPSFMGRGLIHLQKLEISECRAMETIVSKGIETQMQINGDKWDEKNKIEF 900

Query: 901  PELRSLILQHLPELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETL 960
            PELRSLILQHLPELM FY ++ S AVPSTK D+   IS +K  FHPLLN++VSFPKLETL
Sbjct: 901  PELRSLILQHLPELMSFYSYD-SLAVPSTKRDSDQIISAIKTGFHPLLNQQVSFPKLETL 960

Query: 961  KLHALNSGKIWQDQLPASFSGVKNLTSLSVEGCASIKYLMTITMAKSLVHLERLEISNCK 1020
            KLHALN+GKIWQDQ+P+SFSGVKNLTSLSV  CASIKYLMTIT+A+SLV+LERLEI+NCK
Sbjct: 961  KLHALNAGKIWQDQVPSSFSGVKNLTSLSVVDCASIKYLMTITVAQSLVNLERLEINNCK 1020

Query: 1021 LMKDITISEARDLGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLK 1080
            +M+ I ISE +DL +++ ++SI QN+NVFPSLESLV+SRMD+LETLWDKEATSG+F+KLK
Sbjct: 1021 MMQAIIISEDKDLDNNHPSKSILQNQNVFPSLESLVVSRMDALETLWDKEATSGSFTKLK 1080

Query: 1081 KVDIRHCRRLETIFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHL 1140
            KVDIRHC++LET+FP+YMLNRLTNLERL VTDCSS+VEIF+VK  V NG QVAA+AANHL
Sbjct: 1081 KVDIRHCKKLETVFPNYMLNRLTNLERLNVTDCSSVVEIFEVKVPVINGKQVAAIAANHL 1140

Query: 1141 KELKLLRLPKLKHIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQ 1200
            KELKLLRLPKLKHIWS+DP R LSYPSLQ+VHTIHCQSL+N+FP SIAKDL QLEELKIQ
Sbjct: 1141 KELKLLRLPKLKHIWSTDPQRSLSYPSLQLVHTIHCQSLLNIFPVSIAKDLFQLEELKIQ 1200

Query: 1201 FCGVEEIVSKGGGED-----EDAAFVLTGLISLHLWNLFEFKRFYPGKYTLDCPSLQLLD 1260
            FCGVEEIV+KGG E      EDA+FVL+GL SL LWNLFEFKRFYPGKYTLDCPSL LLD
Sbjct: 1201 FCGVEEIVAKGGAEGDGDEYEDASFVLSGLTSLSLWNLFEFKRFYPGKYTLDCPSLTLLD 1260

Query: 1261 VRHCKSFKLLEGALEKLS-PPADDKVEDDQSSKKEQLERRESDVLHEMSTSKKEERTTTT 1320
            VRHCKSFKL+EG  E  +   A +KVEDD+S+++ ++ERRE+DVL E ++++KEE TTT 
Sbjct: 1261 VRHCKSFKLMEGTSESSTLSSALEKVEDDESTQRGKIERREADVLKEETSTRKEEITTTV 1320

Query: 1321 AQEDKVEAELTEIRAQLQTLVMGQNQMMEHLAQLTAIARQTASR 1347
              +D VEAEL EIRAQLQ LV+GQNQMMEHLAQLT IAR+  S+
Sbjct: 1321 --QDNVEAELIEIRAQLQALVLGQNQMMEHLAQLTTIAREGISK 1361

BLAST of MS021966 vs. NCBI nr
Match: QDO73364.1 (disease resistance protein PRS2 [Cucurbita ficifolia])

HSP 1 Score: 2136.3 bits (5534), Expect = 0.0e+00
Identity = 1113/1367 (81.42%), Postives = 1217/1367 (89.03%), Query Frame = 0

Query: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
            MEICS AIVGPIGE+ITNC VDPVFRQLDYL+HFRSNV NL DQ KKLVETRDFVQH+VD
Sbjct: 1    MEICSAAIVGPIGEKITNCAVDPVFRQLDYLVHFRSNVNNLSDQRKKLVETRDFVQHAVD 60

Query: 61   SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
            SAKSNGDEIEGMVVEWLS  +KFS++VDRFFNEANGRSLRWWNM SRHRFSRRA K+A+E
Sbjct: 61   SAKSNGDEIEGMVVEWLSITKKFSDEVDRFFNEANGRSLRWWNMLSRHRFSRRATKMAME 120

Query: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180
            VVKAIEEGRFERVAYR+PP GI  + NSKKFEAFESRV I KEIIEALADA ASVIVVHG
Sbjct: 121  VVKAIEEGRFERVAYRIPPQGIMMLNNSKKFEAFESRVLILKEIIEALADANASVIVVHG 180

Query: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240
            MGGVGKTTL EE+ARL IE KLFDA+AM TVTQ P+VKRIQGEIADQLGLKFEEE +RVR
Sbjct: 181  MGGVGKTTLVEEIARLVIEGKLFDALAMTTVTQIPNVKRIQGEIADQLGLKFEEEKDRVR 240

Query: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300
            A+RLRRRLEMEKKVLVILDDVWAKLDLE +G S HHKGCKILVTSRKDDL+F DFGTQKN
Sbjct: 241  ADRLRRRLEMEKKVLVILDDVWAKLDLEDVGISSHHKGCKILVTSRKDDLYFGDFGTQKN 300

Query: 301  IKINVLSKKEARNFFNKMACDFVD-YNDSDPEMEAVATELADECAGLPLALATVAQALKG 360
            IKI+VL+KKEARNFFNKMACDFV+  NDSDPEMEAVATELADECAGLPLALATVAQALKG
Sbjct: 301  IKIDVLAKKEARNFFNKMACDFVESSNDSDPEMEAVATELADECAGLPLALATVAQALKG 360

Query: 361  KGRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQI 420
            KGRPIWNDAL ELKFP QPNNYGVNKE YSSLKLSYKSL+RDEARSLFLLCSLFP+DYQI
Sbjct: 361  KGRPIWNDALHELKFPSQPNNYGVNKEAYSSLKLSYKSLNRDEARSLFLLCSLFPQDYQI 420

Query: 421  NIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA 480
            NIKYLLMYAMGLGLLNGM+SL  A+ RILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA
Sbjct: 421  NIKYLLMYAMGLGLLNGMNSLAAAKWRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA 480

Query: 481  ILVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGK 540
            IL+ASKMKSKYLVRHG GE +WP +DEFKDYTAISLGCSD +ELPEF CPQL+FLLLVGK
Sbjct: 481  ILIASKMKSKYLVRHGSGESLWPSMDEFKDYTAISLGCSDHSELPEFICPQLRFLLLVGK 540

Query: 541  RTPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELK 600
            RT LRLPE FFAGMQELRVLDLT LCI +LPSSI++LVNL TLCLDDCVLPDMSIVGELK
Sbjct: 541  RTSLRLPEKFFAGMQELRVLDLTSLCIQRLPSSIDELVNLHTLCLDDCVLPDMSIVGELK 600

Query: 601  KLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFK 660
            KLEILSLRASDI+ALPRVIG+LTNLK+LNLSNC KLKVIPANLLS+LTGL ELY+DNSFK
Sbjct: 601  KLEILSLRASDIIALPRVIGELTNLKVLNLSNCFKLKVIPANLLSKLTGLSELYMDNSFK 660

Query: 661  LWNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDW 720
             WNVGH NEGNINA I+ELNNLPRLTTLHVHI +  +LP AS+F NL GYRILIGDGWDW
Sbjct: 661  HWNVGHMNEGNINARISELNNLPRLTTLHVHIPNPTLLPNASVFGNLSGYRILIGDGWDW 720

Query: 721  SGKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDEL--RSAKNILFSLDHKGFPKLK 780
            SGK+ETSKTLKLKLE   QREDAIQALLENTE+LYLDEL   SA++ILFSLDHKGFPKLK
Sbjct: 721  SGKFETSKTLKLKLERGIQREDAIQALLENTENLYLDELGSASARDILFSLDHKGFPKLK 780

Query: 781  RLRVHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVK 840
             LRV NN+EIV+VVNS++MQH HSAFPLLESL LKNL  LG ICRG LPQMSFRNLK VK
Sbjct: 781  CLRVKNNSEIVSVVNSDSMQHLHSAFPLLESLFLKNLDELGSICRGRLPQMSFRNLKIVK 840

Query: 841  VESCSRLKFVFPPSMG-GGLV-LQKLEISECPVMETVVS--------IHDDKMDEKNMIE 900
            VESC+RLKFVFP  MG GGL+ LQKLEISEC  MET+VS        I+ DK DEKN IE
Sbjct: 841  VESCNRLKFVFPSFMGRGGLIHLQKLEISECRAMETIVSKGIETQMQINGDKWDEKNKIE 900

Query: 901  FPELRSLILQHLPELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLET 960
            FPELRSLILQHLPELM FYC++ S AVPSTK D+   IS +K  FHPLLN++VSFPKLET
Sbjct: 901  FPELRSLILQHLPELMSFYCYD-SLAVPSTKRDSDQIISAIKTGFHPLLNQQVSFPKLET 960

Query: 961  LKLHALNSGKIWQDQLPASFSGVKNLTSLSVEGCASIKYLMTITMAKSLVHLERLEISNC 1020
            LKLHALN+GKIWQDQ+P+SFSGVKNLTSLSV  CASIKYLMTIT+A+SLV+LERLEI+NC
Sbjct: 961  LKLHALNAGKIWQDQVPSSFSGVKNLTSLSVVDCASIKYLMTITVAQSLVNLERLEINNC 1020

Query: 1021 KLMKDITISEARDLGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKL 1080
            K+M+ I ISE +DL +++ ++SI QN+NVFPSLESLVISRMD+LETLWDKEATSG+F+KL
Sbjct: 1021 KMMQAIIISEDKDLDNNHPSKSILQNQNVFPSLESLVISRMDALETLWDKEATSGSFTKL 1080

Query: 1081 KKVDIRHCRRLETIFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANH 1140
            KKVDI  C++LET+FP+YMLNRLTNLERL VTDCSS+VEIFQVK  V NG QVAA+AANH
Sbjct: 1081 KKVDIGQCKKLETVFPNYMLNRLTNLERLNVTDCSSVVEIFQVKVPVINGKQVAAIAANH 1140

Query: 1141 LKELKLLRLPKLKHIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKI 1200
            LKELKLLRLPKLKHIWS+DP RFLSYPSLQ+VHTIHCQSL+N+FP SIAKDL QLEELKI
Sbjct: 1141 LKELKLLRLPKLKHIWSTDPQRFLSYPSLQLVHTIHCQSLLNIFPVSIAKDLFQLEELKI 1200

Query: 1201 QFCGVEEIVSKGGGED-----EDAAFVLTGLISLHLWNLFEFKRFYPGKYTLDCPSLQLL 1260
            QFCGVEEIV+KGG E      EDA+FVL+GL SL LWNLFEFKRFYPGKYTLDCPSL LL
Sbjct: 1201 QFCGVEEIVAKGGAEGDGDEYEDASFVLSGLTSLSLWNLFEFKRFYPGKYTLDCPSLTLL 1260

Query: 1261 DVRHCKSFKLLEGALEKLS-PPADDKVEDDQSSKKEQLERRE--SDVLHEMSTSKKEERT 1320
            DVRHCKSFKL+EG  E  +   A +KVEDDQS+++ ++ERRE  +DVL E ST +KEE T
Sbjct: 1261 DVRHCKSFKLMEGTSENSTLSSALEKVEDDQSTQRGKIERREADADVLKETST-RKEEIT 1320

Query: 1321 TTTAQEDKVEAELTEIRAQLQTLVMGQNQMMEHLAQLTAIARQTASR 1347
            TT   +D VEAEL EIRAQLQ LV+GQNQMMEHLAQLT  A QT S+
Sbjct: 1321 TTV--QDNVEAELIEIRAQLQALVVGQNQMMEHLAQLTTTAHQTISK 1363

BLAST of MS021966 vs. NCBI nr
Match: XP_022997552.1 (disease resistance protein RPS2-like [Cucurbita maxima])

HSP 1 Score: 2125.9 bits (5507), Expect = 0.0e+00
Identity = 1099/1364 (80.57%), Postives = 1210/1364 (88.71%), Query Frame = 0

Query: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
            MEICS AIVGPIGE+ITNC VDPVFRQLDYL+HFRSNV NL DQ KKLVETRDFVQH+VD
Sbjct: 1    MEICSAAIVGPIGEKITNCTVDPVFRQLDYLVHFRSNVNNLSDQRKKLVETRDFVQHAVD 60

Query: 61   SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
            SAKSNGD+IEGMVVEWLS  +KF ++VD FFNEANGRSLRWWNM SRHRFSRRA K+A E
Sbjct: 61   SAKSNGDQIEGMVVEWLSITKKFCDEVDSFFNEANGRSLRWWNMLSRHRFSRRATKMATE 120

Query: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180
            VVK IEEGRFERVAYR+PP GI  + NSKKFEAFESR+ I KEIIEALADA ASVIVVHG
Sbjct: 121  VVKTIEEGRFERVAYRIPPQGIMMLNNSKKFEAFESRILILKEIIEALADANASVIVVHG 180

Query: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240
            MGGVGKTTL EE+ARL IE KLFDA+AM TVTQ P+VKRIQGEIADQLGLKFEEE +RVR
Sbjct: 181  MGGVGKTTLVEEIARLVIEGKLFDALAMTTVTQIPNVKRIQGEIADQLGLKFEEEKDRVR 240

Query: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300
            A+RLRRRLEMEKKVLVILDDVWA LDLE IG S HHKGCKILVTSRKDDL+F DFGTQKN
Sbjct: 241  ADRLRRRLEMEKKVLVILDDVWANLDLEDIGISSHHKGCKILVTSRKDDLYFGDFGTQKN 300

Query: 301  IKINVLSKKEARNFFNKMACDFVD-YNDSDPEMEAVATELADECAGLPLALATVAQALKG 360
            IKI+VL+KKEARNFFNKMACDFV+  NDSDPEMEAVATELADECAGLPLALATVAQALKG
Sbjct: 301  IKIDVLAKKEARNFFNKMACDFVESSNDSDPEMEAVATELADECAGLPLALATVAQALKG 360

Query: 361  KGRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQI 420
            KGRPIWNDAL ELKFP QPNNYGVNKE YSSLKLSYKSL+RDEARSLFLLCSLFP+DYQI
Sbjct: 361  KGRPIWNDALHELKFPSQPNNYGVNKEAYSSLKLSYKSLNRDEARSLFLLCSLFPQDYQI 420

Query: 421  NIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA 480
            NIKYLLMYAMGLGLLNGM+SL  A+ RILSLVDELKTSYLLLDG DSDFVKMHDIVRDTA
Sbjct: 421  NIKYLLMYAMGLGLLNGMNSLAAAKWRILSLVDELKTSYLLLDGVDSDFVKMHDIVRDTA 480

Query: 481  ILVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGK 540
            IL+ASKMKSKYLVRHG GE +WPP+DEFKDYTAISLGCSD +ELPEF CPQL+FLLLVGK
Sbjct: 481  ILIASKMKSKYLVRHGAGESLWPPMDEFKDYTAISLGCSDHSELPEFICPQLRFLLLVGK 540

Query: 541  RTPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELK 600
            RT LRLPE FFAGMQELRVLDLT LCI QLPSSI+QLVNL TLCLDDCVLPDMSIVGELK
Sbjct: 541  RTSLRLPEKFFAGMQELRVLDLTSLCIQQLPSSIDQLVNLHTLCLDDCVLPDMSIVGELK 600

Query: 601  KLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFK 660
            KLEILSLRASDI+ALPRVIG+LTNLK+LNLSNC KLKVIPANLLS+LTGL ELY+DNSFK
Sbjct: 601  KLEILSLRASDIIALPRVIGELTNLKVLNLSNCFKLKVIPANLLSKLTGLSELYMDNSFK 660

Query: 661  LWNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDW 720
             WNVGH NEGNINA I+ELN LPRLTTLHVHI +  +LP AS+F NL GYRILIGDGWDW
Sbjct: 661  HWNVGHMNEGNINARISELNYLPRLTTLHVHIPNPILLPNASVFGNLTGYRILIGDGWDW 720

Query: 721  SGKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDEL--RSAKNILFSLDHKGFPKLK 780
            SGK+ETSKTLKLKLE   QREDAIQALLENTE+LYLDEL   SA++IL+SLDHKGFPKLK
Sbjct: 721  SGKFETSKTLKLKLERGNQREDAIQALLENTENLYLDELGSASARDILYSLDHKGFPKLK 780

Query: 781  RLRVHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVK 840
             LRV NN+EIV+VVNS++MQH HSAFPLLESL LKNL  LG ICRG LPQMSFRNLK VK
Sbjct: 781  CLRVKNNSEIVSVVNSDSMQHLHSAFPLLESLFLKNLDELGSICRGRLPQMSFRNLKIVK 840

Query: 841  VESCSRLKFVFPPSMGGGLV-LQKLEISECPVMETVVS--------IHDDKMDEKNMIEF 900
            VESC+RLKFVFP  MG GL+ LQKLEISEC  MET+VS        I+ DK  EKN IEF
Sbjct: 841  VESCNRLKFVFPSFMGRGLIHLQKLEISECRAMETIVSKGIETQMQINGDKWVEKNKIEF 900

Query: 901  PELRSLILQHLPELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETL 960
            PELRSLILQHLPELM FYC++ S AVPSTK D+   IS +K  FHPLLN++VSFPKLETL
Sbjct: 901  PELRSLILQHLPELMSFYCYD-SLAVPSTKRDSDQIISAIKTGFHPLLNQQVSFPKLETL 960

Query: 961  KLHALNSGKIWQDQLPASFSGVKNLTSLSVEGCASIKYLMTITMAKSLVHLERLEISNCK 1020
            KLHALN+GKIWQDQ+P+SFSGVKNLTSLSV  CASIKYLMTIT+A+SLV+LERLEI+NCK
Sbjct: 961  KLHALNAGKIWQDQVPSSFSGVKNLTSLSVVDCASIKYLMTITVAQSLVNLERLEINNCK 1020

Query: 1021 LMKDITISEARDLGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLK 1080
            +M+ I ISE +DL +++ ++SI QN+NVFPSLESLV+SRMD+LETLWDKEATSG+FSKLK
Sbjct: 1021 MMQAIIISEDKDLDNNHPSKSILQNQNVFPSLESLVVSRMDALETLWDKEATSGSFSKLK 1080

Query: 1081 KVDIRHCRRLETIFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHL 1140
            KVDIRHC++LET+FP++MLNRLTNLERL V DCSS+VEIF+VK  V NG QVAA+AANHL
Sbjct: 1081 KVDIRHCKKLETVFPNFMLNRLTNLERLNVIDCSSVVEIFEVKVPVINGKQVAAIAANHL 1140

Query: 1141 KELKLLRLPKLKHIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQ 1200
            KELKLLRLPKLKHIWS+DP RFL+YPSLQ+VHTIHC+SL+N+FP SIAKDL QLEELKIQ
Sbjct: 1141 KELKLLRLPKLKHIWSTDPQRFLNYPSLQLVHTIHCRSLLNIFPLSIAKDLFQLEELKIQ 1200

Query: 1201 FCGVEEIVSKGGGED-----EDAAFVLTGLISLHLWNLFEFKRFYPGKYTLDCPSLQLLD 1260
            FCGVEEIV+KGG E      EDA+FVL+GL SL LWNLFEFKRFYPGKYTLDCPSL LLD
Sbjct: 1201 FCGVEEIVAKGGAEGDGDEYEDASFVLSGLTSLSLWNLFEFKRFYPGKYTLDCPSLTLLD 1260

Query: 1261 VRHCKSFKLLEGALEKLS-PPADDKVEDDQSSKKEQLERRESDVLHEMSTSKKEERTTTT 1320
            VRHCKSFKL+EG  E  +   A +K EDD+S+++ ++ERRE+DVL E + ++KEE TTT 
Sbjct: 1261 VRHCKSFKLMEGTSESSTLSSALEKAEDDESTQRGKIERREADVLKEETFTRKEEITTTV 1320

Query: 1321 AQEDKVEAELTEIRAQLQTLVMGQNQMMEHLAQLTAIARQTASR 1347
              +D VEAEL EIRAQLQ LV+GQNQMMEHLAQLT IAR+  S+
Sbjct: 1321 --QDNVEAELIEIRAQLQALVVGQNQMMEHLAQLTTIAREGISK 1361

BLAST of MS021966 vs. NCBI nr
Match: KAG6598945.1 (Disease resistance protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2122.1 bits (5497), Expect = 0.0e+00
Identity = 1100/1364 (80.65%), Postives = 1210/1364 (88.71%), Query Frame = 0

Query: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
            MEICS AIVGPIGE+ITNC VDPVFRQLDYL+HFRSNV NL DQ KKLVETRDFVQH+VD
Sbjct: 1    MEICSAAIVGPIGEKITNCTVDPVFRQLDYLVHFRSNVNNLSDQRKKLVETRDFVQHAVD 60

Query: 61   SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
            SAKSNGDEIEGMVVEWLS  +KFS++VDRFFNEAN RSLRWWNM SRHRFSRRA K+A+E
Sbjct: 61   SAKSNGDEIEGMVVEWLSITKKFSDEVDRFFNEANVRSLRWWNMLSRHRFSRRATKMAME 120

Query: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180
            VVKAIEEGRFERVAYR+PP GI  + NSKKFEAFESRV I KEIIEALADA ASVIVVHG
Sbjct: 121  VVKAIEEGRFERVAYRIPPQGIMMLNNSKKFEAFESRVLILKEIIEALADANASVIVVHG 180

Query: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240
            MGGVGKTTL EE+ARL  E KLFDA+AM TVTQ P+VKRIQGEIADQLGLKFEEE +RVR
Sbjct: 181  MGGVGKTTLVEEIARLVTEGKLFDALAMTTVTQIPNVKRIQGEIADQLGLKFEEEKDRVR 240

Query: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300
            A+RLRRRLEMEKKVLVILDDVWAKLDLE +G S HHKGCKILVTSRKDDL+F DFGTQKN
Sbjct: 241  ADRLRRRLEMEKKVLVILDDVWAKLDLEDVGISSHHKGCKILVTSRKDDLYFGDFGTQKN 300

Query: 301  IKINVLSKKEARNFFNKMACDFVD-YNDSDPEMEAVATELADECAGLPLALATVAQALKG 360
            IKI+VL+KKEARNFFNKMACDFV+  NDSDPEMEAVATELADECAGLPLALATVAQALKG
Sbjct: 301  IKIDVLAKKEARNFFNKMACDFVESSNDSDPEMEAVATELADECAGLPLALATVAQALKG 360

Query: 361  KGRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQI 420
            KGRPIWNDAL ELKFP QPNNYGVNKE YSSLKLSYKSL+RDEARSLFLLCSLFP+DYQI
Sbjct: 361  KGRPIWNDALHELKFPSQPNNYGVNKEAYSSLKLSYKSLNRDEARSLFLLCSLFPQDYQI 420

Query: 421  NIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA 480
            NIKYLLMYAMGLGLLNGM+SL  A+ RILSLVDELKTSYLLLDG DSDFVKMHDIVRDTA
Sbjct: 421  NIKYLLMYAMGLGLLNGMNSLAAAKWRILSLVDELKTSYLLLDGVDSDFVKMHDIVRDTA 480

Query: 481  ILVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGK 540
            IL+ASKMKSKYLVRHG GE +WPP+DEFKDYTAISLGCSD TELPEF CPQL+FLLLVGK
Sbjct: 481  ILIASKMKSKYLVRHGAGESLWPPMDEFKDYTAISLGCSDHTELPEFICPQLRFLLLVGK 540

Query: 541  RTPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELK 600
            RT LRLPE+FFAGMQELRVLDLT LCI QLPSSI+QLVNL TLCLDDCVLPDMSIVGELK
Sbjct: 541  RTSLRLPENFFAGMQELRVLDLTSLCIQQLPSSIDQLVNLHTLCLDDCVLPDMSIVGELK 600

Query: 601  KLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFK 660
            KLEILSLRASDI+ALPRVIG+LTNLK+LNLSNC KLKVIPANLLS+LTGL ELY+DNSFK
Sbjct: 601  KLEILSLRASDIIALPRVIGELTNLKVLNLSNCFKLKVIPANLLSKLTGLSELYMDNSFK 660

Query: 661  LWNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDW 720
             WNVGH NEGNINA I+ELN LPRLTTLHVHI +  +LP AS+F NL GYRILIGDGWDW
Sbjct: 661  HWNVGHMNEGNINARISELNYLPRLTTLHVHIPNPTLLPNASVFGNLSGYRILIGDGWDW 720

Query: 721  SGKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDEL--RSAKNILFSLDHKGFPKLK 780
            SGK+ETSKTLKLKLE   QREDAIQALLENTE+LYLDEL   SA++IL+SLDHKGFPKLK
Sbjct: 721  SGKFETSKTLKLKLERGIQREDAIQALLENTENLYLDELGSASARDILYSLDHKGFPKLK 780

Query: 781  RLRVHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVK 840
             LRV NN+EIV+VV S+ MQH HSAFPLLESL LKNL  L  ICRG LPQMSFRNLK VK
Sbjct: 781  CLRVKNNSEIVSVVKSDGMQHLHSAFPLLESLFLKNLDELASICRGRLPQMSFRNLKIVK 840

Query: 841  VESCSRLKFVFPPSMGGGLV-LQKLEISECPVMETVVS--------IHDDKMDEKNMIEF 900
            VESC+RLKFVFP  MG GL+ LQKLEISEC  MET+VS        I+ DK DEKN IEF
Sbjct: 841  VESCNRLKFVFPSFMGRGLIHLQKLEISECRAMETIVSKGIETQMQINGDKWDEKNKIEF 900

Query: 901  PELRSLILQHLPELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETL 960
            PELRSLILQHLPELM FY ++ S AVPSTK D+   IS +K  FHPLLN++VSFPKLETL
Sbjct: 901  PELRSLILQHLPELMSFYSYD-SLAVPSTKRDSDQIISAIKTGFHPLLNQQVSFPKLETL 960

Query: 961  KLHALNSGKIWQDQLPASFSGVKNLTSLSVEGCASIKYLMTITMAKSLVHLERLEISNCK 1020
            KLH+LN+GKIWQDQ+P   SGVKNLTSLSV  CASIKYLMTIT+A+SLV+LERLEI+NCK
Sbjct: 961  KLHSLNAGKIWQDQVP---SGVKNLTSLSVVDCASIKYLMTITVAQSLVNLERLEINNCK 1020

Query: 1021 LMKDITISEARDLGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLK 1080
            +M+ I ISE +DL ++  ++SI QN+NVFPSLESLV+SRMD+LETLWDKEATSG+F+KLK
Sbjct: 1021 MMQAIIISEDKDLDNNRPSKSILQNQNVFPSLESLVVSRMDALETLWDKEATSGSFTKLK 1080

Query: 1081 KVDIRHCRRLETIFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHL 1140
            KVDIRHC++LET+FP+YMLNRLTNLERL VTDCSS+VEIF+VK  V NG QVAA+AANHL
Sbjct: 1081 KVDIRHCKKLETVFPNYMLNRLTNLERLNVTDCSSVVEIFEVKVPVINGKQVAAIAANHL 1140

Query: 1141 KELKLLRLPKLKHIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQ 1200
            +ELKLLRLPKLKHIWS+DP RFLSYPSLQ+VHTIHCQSL+N+FP SIAKDL QLEELKIQ
Sbjct: 1141 RELKLLRLPKLKHIWSTDPQRFLSYPSLQLVHTIHCQSLLNIFPVSIAKDLFQLEELKIQ 1200

Query: 1201 FCGVEEIVSKGGGED-----EDAAFVLTGLISLHLWNLFEFKRFYPGKYTLDCPSLQLLD 1260
            FCGVEEIV+KGG E      EDA+FVL+GL SL LWNLFEFKRFYPGKYTLDCPSL LLD
Sbjct: 1201 FCGVEEIVAKGGAEGDGDEYEDASFVLSGLTSLSLWNLFEFKRFYPGKYTLDCPSLTLLD 1260

Query: 1261 VRHCKSFKLLEGALEKLS-PPADDKVEDDQSSKKEQLERRESDVLHEMSTSKKEERTTTT 1320
            VRHCKSFKL+EG  E  +   A +KVEDD+S+++ ++ERRE+DVL E ++++KEE TTT 
Sbjct: 1261 VRHCKSFKLMEGTSESSTLSSALEKVEDDESTQRGKIERREADVLKEETSTRKEEITTTV 1320

Query: 1321 AQEDKVEAELTEIRAQLQTLVMGQNQMMEHLAQLTAIARQTASR 1347
              +D VEAEL EIRAQLQ LV+GQNQMMEHLAQLT +AR+  S+
Sbjct: 1321 --QDNVEAELIEIRAQLQALVLGQNQMMEHLAQLTTMAREGISK 1358

BLAST of MS021966 vs. ExPASy Swiss-Prot
Match: Q9T048 (Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g27190 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 1.2e-72
Identity = 265/941 (28.16%), Postives = 442/941 (46.97%), Query Frame = 0

Query: 1   MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
           ME C+     P+   I   M +  F ++   + F+SNV  L + L++L E +  +    +
Sbjct: 1   MECCA-----PVIGEILRLMYESTFSRVANAIKFKSNVKALNESLERLTELKGNMSEDHE 60

Query: 61  SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
           +  +    +   ++ W   AE+          E          M  R R SR+ VK+ ++
Sbjct: 61  TLLTKDKPLRLKLMRWQREAEEVISKARLKLEERVS-----CGMSLRPRMSRKLVKI-LD 120

Query: 121 VVKAIEEGRFERV---AYRLPP------PGIFTVKNSKKFEAFESRVPIQKEIIEALADA 180
            VK +E+   E V   +    P      PG+  V  +           +  +I + L   
Sbjct: 121 EVKMLEKDGIEFVDMLSVESTPERVEHVPGVSVVHQTMASN-------MLAKIRDGLTSE 180

Query: 181 YASVIVVHGMGGVGKTTLAEEVARLAIE---CKLFDAVAMATVTQTPDVKRIQGEIADQL 240
            A  I V GMGGVGKTTL   +     E    + F  V    V++  D + +Q +IA++L
Sbjct: 181 KAQKIGVWGMGGVGKTTLVRTLNNKLREEGATQPFGLVIFVIVSKEFDPREVQKQIAERL 240

Query: 241 GLKFE-EESERVRANRLRRRLEMEKKVLVILDDVWAKLDLEAIG--TSGHHKGCKILVTS 300
            +  + EESE   A R+   L  E+K L+ILDDVW  +DL+ +G   +  +KG K+++TS
Sbjct: 241 DIDTQMEESEEKLARRIYVGLMKERKFLLILDDVWKPIDLDLLGIPRTEENKGSKVILTS 300

Query: 301 RKDDLFFDDFGTQKNIKINVLSKKEARNFFNKMACDFVDYNDSDPEMEAVATELADECAG 360
           R  ++      T  +++++ L +++A   F K A D V        +  +A  ++ EC G
Sbjct: 301 RFLEV-CRSMKTDLDVRVDCLLEEDAWELFCKNAGDVV----RSDHVRKIAKAVSQECGG 360

Query: 361 LPLALATVAQALKGKGR-PIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEAR 420
           LPLA+ TV  A++GK    +WN  L +L     P    + ++++  LKLSY  L+ D+A+
Sbjct: 361 LPLAIITVGTAMRGKKNVKLWNHVLSKLS-KSVPWIKSIEEKIFQPLKLSYDFLE-DKAK 420

Query: 421 SLFLLCSLFPEDYQINIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGA 480
             FLLC+LFPEDY I +  ++ Y M  G +  + S   +    ++ V+ LK   LL DG 
Sbjct: 421 FCFLLCALFPEDYSIEVTEVVRYWMAEGFMEELGSQEDSMNEGITTVESLKDYCLLEDGD 480

Query: 481 DSDFVKMHDIVRDTAILVASKMK--SKYLVRHGVG------EKMWPPIDEFKDYTAISLG 540
             D VKMHD+VRD AI + S  +  S  LV  G G      +K+ P +   +  + ++  
Sbjct: 481 RRDTVKMHDVVRDFAIWIMSSSQDDSHSLVMSGTGLQDIRQDKLAPSL---RRVSLMNNK 540

Query: 541 CSDFTELPEFTCPQLKFLLLVGKRTPLRLPESFFAGMQELRVLDLTGLCIVQLPS-SINQ 600
                +L E  C +   LLL G      +P  F      LR+L+L+G  I   PS S+ +
Sbjct: 541 LESLPDLVEEFCVKTSVLLLQGNFLLKEVPIGFLQAFPTLRILNLSGTRIKSFPSCSLLR 600

Query: 601 LVNLQTLCLDDCV-LPDMSIVGELKKLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSK 660
           L +L +L L DC  L  +  +  L KLE+L L  + I+  PR + +L   + L+LS    
Sbjct: 601 LFSLHSLFLRDCFKLVKLPSLETLAKLELLDLCGTHILEFPRGLEELKRFRHLDLSRTLH 660

Query: 661 LKVIPANLLSRLTGLCELYLDNSFKLWNV-GHANEGNINAGIAELNNLPRLTTLHV--HI 720
           L+ IPA ++SRL+ L  L + +S   W+V G   +G   A + E+  L RL  L +  H 
Sbjct: 661 LESIPARVVSRLSSLETLDMTSSHYRWSVQGETQKG--QATVEEIGCLQRLQVLSIRLHS 720

Query: 721 SDARILPRASLFRNLIGYRILIGDGWDWSGKYETSKTLKLKLESSFQREDAIQALLENTE 780
           S   +  R +  + L  +++++G  +    +++  +     L  S   + +I  LL  T 
Sbjct: 721 SPFLLNKRNTWIKRLKKFQLVVGSRYILRTRHDKRRLTISHLNVS---QVSIGWLLAYTT 780

Query: 781 DLYLDELRSAKNILFSL--DHKGFPKLKRLRVHNN-------AEIVTVVNSENMQHPHSA 840
            L L+  +  + ++  L  D+KGF  LK L + N         E+V+   S+        
Sbjct: 781 SLALNHCQGIEAMMKKLVSDNKGFKNLKSLTIENVIINTNSWVEMVSTNTSKQSSDILDL 840

Query: 841 FPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVKVESCSRLKFVFPPSMGGGLVLQKLE 900
            P LE L L+ +         T   +    LK +++  C +L+ +        L +  LE
Sbjct: 841 LPNLEELHLRRVDLETFSELQTHLGLKLETLKIIEITMCRKLRTLL--DKRNFLTIPNLE 900

Query: 901 ISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLPELM 904
             E    +++ ++H+  +  +  +  P LR L L++LP L+
Sbjct: 901 EIEISYCDSLQNLHEALLYHQPFV--PNLRVLKLRNLPNLV 904

BLAST of MS021966 vs. ExPASy Swiss-Prot
Match: O81825 (Probable disease resistance protein At4g27220 OS=Arabidopsis thaliana OX=3702 GN=At4g27220 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.9e-67
Identity = 255/895 (28.49%), Postives = 411/895 (45.92%), Query Frame = 0

Query: 34  FRSNVTNLRDQLKKLVETRDFVQHSVDSAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNE 93
           FRSN   L   L++L   +  V  ++  +      +E  +  WL   E+ +  +     E
Sbjct: 2   FRSNARALNRALERLKNVQTKVNEALKRSGIQEKSLERKLRIWLRKVEE-NVPLGELILE 61

Query: 94  ANGRSLRWWNMPSRHRFSRRAVKLAVEVVKAIEEGR--FERVAYRLPPPGIFTVKNSKKF 153
                  W         S + V++  +V +  E+G+   ++++       I        F
Sbjct: 62  KRSSCAIW--------LSDKDVEILEKVKRLEEQGQDLIKKISVNKSSREIVERVLGPSF 121

Query: 154 EAFESRVPIQKEIIEALADAYASVIVVHGMGGVGKTTLAEEVARLAIE---CKLFDAVAM 213
              ++ + +  ++ + L       I V GMGGVGKTTL   +    ++    + F  V  
Sbjct: 122 HPQKTALEMLDKLKDCLKKKNVQKIGVWGMGGVGKTTLVRTLNNDLLKYAATQQFALVIW 181

Query: 214 ATVTQTPDVKRIQGEIADQLGLKFEEESERVRANRLRRRLEMEKKVLVILDDVWAKLDLE 273
            TV++  D+KR+Q +IA +LG +F  E        +  RL   K  L+ILDDVW  +DL+
Sbjct: 182 VTVSKDFDLKRVQMDIAKRLGKRFTREQMNQLGLTICERLIDLKNFLLILDDVWHPIDLD 241

Query: 274 AIG---TSGHHKGCKILVTSRKDDLFFDDFGTQKNIKINVLSKKEARNFFNKMACDFVDY 333
            +G        K  K+++TSR+ ++      T +NIK+  L +KEA   F    C  V  
Sbjct: 242 QLGIPLALERSKDSKVVLTSRRLEV-CQQMMTNENIKVACLQEKEAWELF----CHNVGE 301

Query: 334 NDSDPEMEAVATELADECAGLPLALATVAQALKGKGR-PIWNDALQELKFPGQPNNYGVN 393
             +   ++ +A +++ EC GLPLA+ T+ + L+GK +  +W   L  LK      +    
Sbjct: 302 VANSDNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLK--RSAPSIDTE 361

Query: 394 KEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQINIKYLLMYAMGLGLLNGMSSLMVAR 453
           ++++ +LKLSY  L +D  +S FL C+LFPEDY I +  L+MY +  GLL+G        
Sbjct: 362 EKIFGTLKLSYDFL-QDNMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQHHYEDMM 421

Query: 454 GRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTAILVASKMKSKY--LVRHGVGEKMWP 513
              ++LV+ LK S LL DG   D VKMHD+VRD AI   S     +  LV  G G   +P
Sbjct: 422 NEGVTLVERLKDSCLLEDGDSCDTVKMHDVVRDFAIWFMSSQGEGFHSLVMAGRGLIEFP 481

Query: 514 PIDEFKDYTAISLGCSDFTELPEFTCPQLK--FLLLVGKRTPLRLPESFFAGMQELRVLD 573
                     +SL  +    LP      ++   LLL G      +P  F      LR+LD
Sbjct: 482 QDKFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGNSHVKEVPNGFLQAFPNLRILD 541

Query: 574 LTGLCIVQLPSSINQLVNLQTLCLDDC-VLPDMSIVGELKKLEILSLRASDIVALPRVIG 633
           L+G+ I  LP S + L +L++L L +C  L ++  +  L KL+ L L  S I  LPR + 
Sbjct: 542 LSGVRIRTLPDSFSNLHSLRSLVLRNCKKLRNLPSLESLVKLQFLDLHESAIRELPRGLE 601

Query: 634 QLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFKLWNV-GHANEGNINAGIAEL 693
            L++L+ + +SN  +L+ IPA  + +L+ L  L +  S   W + G   EG   A + E+
Sbjct: 602 ALSSLRYICVSNTYQLQSIPAGTILQLSSLEVLDMAGSAYSWGIKGEEREG--QATLDEV 661

Query: 694 NNLPRLTTLHVHISDARILPRA--SLFRNLIGYRILIGDGWDWS--GKYETSKTLKLKLE 753
             LP L  L + + D         SL + L  ++ L       S  G  E      L + 
Sbjct: 662 TCLPHLQFLAIKLLDVLSFSYEFDSLTKRLTKFQFLFSPIRSVSPPGTGEGC----LAIS 721

Query: 754 SSFQREDAIQALLENTEDL---YLDELRSAKNILFSLDHKGFPKLKRLRVHNNAEIVTVV 813
                  +I  LL++   L   Y + L      L +     F  +K L +H    +    
Sbjct: 722 DVNVSNASIGWLLQHVTSLDLNYCEGLNGMFENLVTKSKSSFVAMKALSIHYFPSLSLAS 781

Query: 814 NSENMQHPHSAFPLLESLSLK--NLANLGLICRGTLPQMSFRNLKRVKVESCSRLKFVFP 873
             E+       FP LE LSL   NL ++G +  G L  M  + LK ++V  C +LK +F 
Sbjct: 782 GCESQL---DLFPNLEELSLDNVNLESIGEL-NGFL-GMRLQKLKLLQVSGCRQLKRLFS 841

Query: 874 PSMGGGLV--LQKLEISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLPEL 903
             +  G +  LQ++++  C  +E + +     +D       P+L  + L++LP+L
Sbjct: 842 DQILAGTLPNLQEIKVVSCLRLEELFNFSSVPVDFCAESLLPKLTVIKLKYLPQL 868

BLAST of MS021966 vs. ExPASy Swiss-Prot
Match: Q42484 (Disease resistance protein RPS2 OS=Arabidopsis thaliana OX=3702 GN=RPS2 PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 9.0e-57
Identity = 247/892 (27.69%), Postives = 398/892 (44.62%), Query Frame = 0

Query: 35  RSNVTNLRDQLKKLVETRDFVQHSVDSAKSNGDEIEGMVVEWLSFAE----KFSEDVDRF 94
           R  +T+L   +  L   RD +   +      G        EWLS  +    K +  + RF
Sbjct: 32  RQAITDLETAIGDLKAIRDDLTLRIQQDGLEGRSCSNRAREWLSAVQVTETKTALLLVRF 91

Query: 95  FNEANGRSLRWWNMPSRHRFSRRAVKLAVEVVKAIEEGRFERVAYRLPPPGIFTVKNSKK 154
                   +R   +        +  K    ++K+I E R    A +     I        
Sbjct: 92  RRREQRTRMRRRYLSCFGCADYKLCKKVSAILKSIGELRERSEAIKTDGGSIQVTCREIP 151

Query: 155 FEAFESRVPIQKEIIEALA-DAYASVIVVHGMGGVGKTTLAEEV-ARLAIECKLFDAVAM 214
            ++      + ++++E L+ +    +I V+G GGVGKTTL + +   L  +   +D +  
Sbjct: 152 IKSVVGNTTMMEQVLEFLSEEEERGIIGVYGPGGVGKTTLMQSINNELITKGHQYDVLIW 211

Query: 215 ATVTQTPDVKRIQGEIADQLGLKFEE-ESERVRANRLRRRLEMEKKVLVILDDVWAKLDL 274
             +++      IQ  +  +LGL ++E E+   RA ++ R L  +K+ L++LDDVW ++DL
Sbjct: 212 VQMSREFGECTIQQAVGARLGLSWDEKETGENRALKIYRALR-QKRFLLLLDDVWEEIDL 271

Query: 275 EAIGT--SGHHKGCKILVTSRKDDLFFDDFGTQKNIKINVLSKKEARNFFNKMACDFVDY 334
           E  G         CK++ T+R   L  ++ G +  +++  L KK A   F    C  V  
Sbjct: 272 EKTGVPRPDRENKCKVMFTTRSIAL-CNNMGAEYKLRVEFLEKKHAWELF----CSKVWR 331

Query: 335 ND--SDPEMEAVATELADECAGLPLALATVAQALKGK-GRPIWNDALQEL-KFPGQPNNY 394
            D      +  +A  +  +C GLPLAL T+  A+  +     W  A + L +FP +    
Sbjct: 332 KDLLESSSIRRLAEIIVSKCGGLPLALITLGGAMAHRETEEEWIHASEVLTRFPAEMK-- 391

Query: 395 GVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQINIKYLLMYAMGLGLLNGMSSL- 454
           G+N  V++ LK SY +L+ D  RS FL C+LFPE++ I I+ L+ Y +G G L     + 
Sbjct: 392 GMN-YVFALLKFSYDNLESDLLRSCFLYCALFPEEHSIEIEQLVEYWVGEGFLTSSHGVN 451

Query: 455 MVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTAILVASKM---KSKYLVRHGVG 514
            + +G    L+ +LK + LL  G +   VKMH++VR  A+ +AS+    K   LV   +G
Sbjct: 452 TIYKGYF--LIGDLKAACLLETGDEKTQVKMHNVVRSFALWMASEQGTYKELILVEPSMG 511

Query: 515 EKMWPPIDEFKDYTAISLGCSDFTELPE-FTCPQLKFLLLVGKRTPLRLPESFFAGMQEL 574
               P  + ++    ISL  +    LPE   CP+L  L+L    +  ++P  FF  M  L
Sbjct: 512 HTEAPKAENWRQALVISLLDNRIQTLPEKLICPKLTTLMLQQNSSLKKIPTGFFMHMPVL 571

Query: 575 RVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELKKLEILSLRASDIVALPR 634
           RVLDL+   I ++P SI  LV L                        LS+  + I  LP+
Sbjct: 572 RVLDLSFTSITEIPLSIKYLVELYH----------------------LSMSGTKISVLPQ 631

Query: 635 VIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFKLWNVGHANEGNI-NAGI 694
            +G L  LK L+L     L+ IP + +  L+ L  L L  S+  W +    E      G 
Sbjct: 632 ELGNLRKLKHLDLQRTQFLQTIPRDAICWLSKLEVLNLYYSYAGWELQSFGEDEAEELGF 691

Query: 695 AELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDWSGKYETSKTLKLKLES 754
           A+L  L  LTTL                    G  +L           ET KTL      
Sbjct: 692 ADLEYLENLTTL--------------------GITVL---------SLETLKTL------ 751

Query: 755 SFQREDAIQALLENTEDLYLDELRSAKNI-LFSLDHKGFPKLKRLRV---HNNAEIVTVV 814
            F+      AL ++ + L+++E        L SL + G   L+RL +   H+   +VT  
Sbjct: 752 -FE----FGALHKHIQHLHVEECNELLYFNLPSLTNHG-RNLRRLSIKSCHDLEYLVTPA 811

Query: 815 NSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVKVESCSRLKFVFPPS 874
           + EN        P LE L+L +L NL  +   ++ Q   RN++ + +  C++LK V   S
Sbjct: 812 DFEN-----DWLPSLEVLTLHSLHNLTRVWGNSVSQDCLRNIRCINISHCNKLKNV---S 840

Query: 875 MGGGL-VLQKLEISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLPEL 903
               L  L+ +E+ +C  +E ++S H+    E   + FP L++L  + LPEL
Sbjct: 872 WVQKLPKLEVIELFDCREIEELISEHESPSVEDPTL-FPSLKTLRTRDLPEL 840

BLAST of MS021966 vs. ExPASy Swiss-Prot
Match: P60838 (Disease resistance protein SUMM2 OS=Arabidopsis thaliana OX=3702 GN=SUMM2 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 5.3e-49
Identity = 219/874 (25.06%), Postives = 402/874 (46.00%), Query Frame = 0

Query: 14  ERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD-----SAKSNGDE 73
           + + N +   +   + Y+     NV  ++  ++ L + RD V+  VD       +    +
Sbjct: 12  DEVVNQISQGLCINVGYICELSKNVVAMKKDMEVLKKKRDDVKRRVDIEEFTRRRERLSQ 71

Query: 74  IEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWW-----NMPSRHRFSRRAVKLAVEVVK 133
           ++G +    +   KF+E +    N+A  + L  +     N+   + + +R V +  E+  
Sbjct: 72  VQGWLTNVSTVENKFNELLTT--NDAELQRLCLFGFCSKNVKMSYLYGKRVVLMLKEIES 131

Query: 134 AIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHGMGG 193
              +G F+ V    P   I  ++          +  + + +   L +    ++ ++GMGG
Sbjct: 132 LSSQGDFDTVTLATP---IARIEEMPIQPTIVGQETMLERVWTRLTEDGDEIVGLYGMGG 191

Query: 194 VGKTTLAEEV-ARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVRAN 253
           VGKTTL   +  + + +C  F  V    V+++PD+ RIQG+I  +L L  EE       N
Sbjct: 192 VGKTTLLTRINNKFSEKCSGFGVVIWVVVSKSPDIHRIQGDIGKRLDLGGEEWD---NVN 251

Query: 254 RLRRRLEM-----EKKVLVILDDVWAKLDLEAIGT--SGHHKGCKILVTSRKDDLFFDDF 313
             +R L++     ++K +++LDD+W K++LE +G        GCK++ T+R  D+     
Sbjct: 252 ENQRALDIYNVLGKQKFVLLLDDIWEKVNLEVLGVPYPSRQNGCKVVFTTRSRDV-CGRM 311

Query: 314 GTQKNIKINVLSKKEARNFFNKMACDFVDYNDSDPEMEAVATELADECAGLPLALATVAQ 373
                ++++ L   EA   F     +  +     P++  +A ++A +C GLPLAL  + +
Sbjct: 312 RVDDPMEVSCLEPNEAWELFQMKVGE--NTLKGHPDIPELARKVAGKCCGLPLALNVIGE 371

Query: 374 ALKGKGR-PIWNDALQEL-----KFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLL 433
            +  K     W +A+  L     +FPG        +++   LK SY +L++++ +  FL 
Sbjct: 372 TMACKRMVQEWRNAIDVLSSYAAEFPGM-------EQILPILKYSYDNLNKEQVKPCFLY 431

Query: 434 CSLFPEDYQINIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGA-DSDF 493
           CSLFPEDY++  + L+ Y +  G ++   S   A  +   ++  L  + LLL+ A + + 
Sbjct: 432 CSLFPEDYRMEKERLIDYWICEGFIDENESRERALSQGYEIIGILVRACLLLEEAINKEQ 491

Query: 494 VKMHDIVRDTAILVASKM---KSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTEL-- 553
           VKMHD+VR+ A+ +AS +   K + +V+ GVG +  P +  +     +SL  ++   L  
Sbjct: 492 VKMHDVVREMALWIASDLGEHKERCIVQVGVGLREVPKVKNWSSVRRMSLMENEIEILSG 551

Query: 554 -PEFTCPQLKFLLLVGKRTPLRLPESFFAGMQELRVLDLTG-LCIVQLPSSINQLVNLQT 613
            PE  C +L  L L    + L + + FF  +  L VLDL+G   + +LP+ I++LV+L+ 
Sbjct: 552 SPE--CLELTTLFLQKNDSLLHISDEFFRCIPMLVVLDLSGNSSLRKLPNQISKLVSLRY 611

Query: 614 LCLDDCVLPDMSI-VGELKKLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPA 673
           L L    +  + + + ELKKL  L L   D +   + I  ++     N+S+  KL+++ +
Sbjct: 612 LDLSWTYIKRLPVGLQELKKLRYLRL---DYMKRLKSISGIS-----NISSLRKLQLLQS 671

Query: 674 NLLSRLTGLCELYLDNSFKLWNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRA 733
            +   ++ + EL L    ++ N+   +    +  + +L N PRL      +   +IL   
Sbjct: 672 KMSLDMSLVEELQLLEHLEVLNISIKS----SLVVEKLLNAPRL------VKCLQILVLR 731

Query: 734 SLFRNLIGYRILIGDGWDWSGKYETSK--TLKLKLES-----SFQREDAIQALLENTEDL 793
            +     G  +L     D   K    K    ++K+E      S  R    Q  L N   +
Sbjct: 732 GVQEESSG--VLTLPDMDNLNKVIIRKCGMCEIKIERKTLSLSSNRSPKTQ-FLHNLSTV 791

Query: 794 YLDELRSAKNILFSLDHKGFPKLKRLRVHNNAEIVTVVNSENMQHPHSAFPL--LESLSL 846
           ++      K++ + L     P L  L V ++  +  ++N E         P   LESL L
Sbjct: 792 HISSCDGLKDLTWLL---FAPNLTSLEVLDSELVEGIINQEKAMTMSGIIPFQKLESLRL 838

BLAST of MS021966 vs. ExPASy Swiss-Prot
Match: Q940K0 (Disease resistance protein UNI OS=Arabidopsis thaliana OX=3702 GN=UNI PE=1 SV=2)

HSP 1 Score: 193.4 bits (490), Expect = 1.7e-47
Identity = 223/800 (27.88%), Postives = 368/800 (46.00%), Query Frame = 0

Query: 106 SRHRFSRRAVKLAVEVVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEII 165
           S +++ ++   L  EV K   EG F+ V+   PPP       S+  E        Q++++
Sbjct: 109 SSYKYGKKVFLLLEEVKKLNSEGNFDEVSQ--PPP------RSEVEERPTQPTIGQEDML 168

Query: 166 E----ALADAYASVIVVHGMGGVGKTTLAEEVARLAIEC-KLFDAVAMATVTQTPDVKRI 225
           E     L +    ++ +HGMGGVGKTTL +++     E    FD V    V++   + ++
Sbjct: 169 EKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAEIGGTFDIVIWIVVSKGVMISKL 228

Query: 226 QGEIADQLGL---KFEEESERVRANRLRRRLEMEKKVLVILDDVWAKLDLEAIGT--SGH 285
           Q +IA++L L    ++ ++E  +A  + R L+  K+ +++LDD+W K+DLEAIG      
Sbjct: 229 QEDIAEKLHLCDDLWKNKNESDKATDIHRVLK-GKRFVLMLDDIWEKVDLEAIGIPYPSE 288

Query: 286 HKGCKILVTSRKDDLFFDDFGTQKNIKINVLSKKEARNFFNKMACDFVDYNDSDPEMEAV 345
              CK+  T+R  ++   + G  K +++N L  ++A   F     D  +   SDP +  +
Sbjct: 289 VNKCKVAFTTRSREV-CGEMGDHKPMQVNCLEPEDAWELFKNKVGD--NTLSSDPVIVEL 348

Query: 346 ATELADECAGLPLALATVAQALKGKGR-PIWNDALQELKFPGQPNNYGVNKEVYSSLKLS 405
           A E+A +C GLPLAL  + + +  K     W  A+          +   NK +   LK S
Sbjct: 349 AREVAQKCRGLPLALNVIGETMSSKTMVQEWEHAIHVFNTSAAEFSDMQNK-ILPILKYS 408

Query: 406 YKSLDRDEARSLFLLCSLFPEDYQINIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDEL 465
           Y SL  +  +S FL C+LFPED +I  + L+ Y +  G +     +  AR +  +++  L
Sbjct: 409 YDSLGDEHIKSCFLYCALFPEDGEIYNEKLIDYWICEGFIGEDQVIKRARNKGYAMLGTL 468

Query: 466 KTSYLLLDGADSDFVKMHDIVRDTAILVAS---KMKSKYLVRHGVGEKMWPPIDEFKDYT 525
            T   LL    + +  MHD+VR+ A+ +AS   K K  ++V+ GVG    P + ++    
Sbjct: 469 -TRANLLTKVGTYYCVMHDVVREMALWIASDFGKQKENFVVQAGVGLHEIPKVKDWGAVR 528

Query: 526 AISLGCSDFTELP-EFTCPQLKFLLLVGKRTPLRLPESFFAGMQELRVLDLT-GLCIVQL 585
            +SL  +D  E+  E  C +L  L L   +    LP +F   MQ+L VLDL+      +L
Sbjct: 529 KMSLMDNDIEEITCESKCSELTTLFLQSNKLK-NLPGAFIRYMQKLVVLDLSYNRDFNKL 588

Query: 586 PSSINQLVNLQTLCLDDCVLPDMSI-VGELKKLEILSLRASDIVALPRVIGQLTNLKMLN 645
           P  I+ LV+LQ L L +  +  M I + ELKKL  L L  +D +     I +L +L++L 
Sbjct: 589 PEQISGLVSLQFLDLSNTSIEHMPIGLKELKKLTFLDLTYTDRLCSISGISRLLSLRLLR 648

Query: 646 LSNCSKLKVIPANL--LSRLTGLCELYLDNSFKLWNVGHANEGNINAGIAELNNLPRLTT 705
           L   SK+    + L  L +L  L EL +  S +L ++              L  L     
Sbjct: 649 LLG-SKVHGDASVLKELQQLQNLQELAITVSAELISLDQ-----------RLAKLISNLC 708

Query: 706 LHVHISDARILPRASLFRNLIGYRILIGDGWDWSGKYETSKTLKLKLESSFQREDAIQAL 765
           +   +     L   +   NL   R+        +  +   K  + + ESS+ R +     
Sbjct: 709 IEGFLQKPFDLSFLASMENLSSLRV-------ENSYFSEIKCRESETESSYLRINPKIPC 768

Query: 766 LENTEDLYLDELRSAKNILFSLDHKGFPKLKRLRVHNNAEIVTVVNSENMQHPHSAFPL- 825
             N   L + +  S K++ + L     P L  L + ++ E+  ++N E   +  S  P  
Sbjct: 769 FTNLSRLEIMKCHSMKDLTWIL---FAPNLVVLLIEDSREVGEIINKEKATNLTSITPFL 828

Query: 826 -LESLSLKNLANLGLICRGTLPQMSFRNLKRVKVESCSRLKFVFPPSMGGGLVLQKLEIS 885
            LE L L NL  L  I    LP   F  L  + V +C +L+   P +      +++ EI 
Sbjct: 829 KLEWLILYNLPKLESIYWSPLP---FPVLLTMDVSNCPKLR-KLPLNATSVSKVEEFEIH 867

BLAST of MS021966 vs. ExPASy TrEMBL
Match: A0A6J1D734 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111018222 PE=4 SV=1)

HSP 1 Score: 2613.2 bits (6772), Expect = 0.0e+00
Identity = 1334/1346 (99.11%), Postives = 1338/1346 (99.41%), Query Frame = 0

Query: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
            MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD
Sbjct: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60

Query: 61   SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
            SAKSNGDEIEGMVVEWLS AEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE
Sbjct: 61   SAKSNGDEIEGMVVEWLSIAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120

Query: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180
            VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG
Sbjct: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180

Query: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240
            MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR
Sbjct: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240

Query: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300
            ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN
Sbjct: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300

Query: 301  IKINVLSKKEARNFFNKMACDFVDYNDSDPEMEAVATELADECAGLPLALATVAQALKGK 360
            IKINVLSKKEARNFFNKMACDFVD NDSDPEMEAVATELADECAGLPLALATVAQALKGK
Sbjct: 301  IKINVLSKKEARNFFNKMACDFVDCNDSDPEMEAVATELADECAGLPLALATVAQALKGK 360

Query: 361  GRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQIN 420
            GRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQIN
Sbjct: 361  GRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQIN 420

Query: 421  IKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTAI 480
            IKYLLMYAMGLGLLNGMSSL+VARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTAI
Sbjct: 421  IKYLLMYAMGLGLLNGMSSLVVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTAI 480

Query: 481  LVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGKR 540
            LVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGKR
Sbjct: 481  LVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGKR 540

Query: 541  TPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELKK 600
            TPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDM IVGELKK
Sbjct: 541  TPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMXIVGELKK 600

Query: 601  LEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFKL 660
            LEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFKL
Sbjct: 601  LEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFKL 660

Query: 661  WNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDWS 720
            WNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDWS
Sbjct: 661  WNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDWS 720

Query: 721  GKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDELRSAKNILFSLDHKGFPKLKRLR 780
            GKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDELRSAKNILFSLDHKGFPKLKRLR
Sbjct: 721  GKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDELRSAKNILFSLDHKGFPKLKRLR 780

Query: 781  VHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVKVES 840
            VHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVKVES
Sbjct: 781  VHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVKVES 840

Query: 841  CSRLKFVFPPSMGGGLVLQKLEISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLP 900
            CSRLKFVFPPSMGGGLVLQKLEISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLP
Sbjct: 841  CSRLKFVFPPSMGGGLVLQKLEISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLP 900

Query: 901  ELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETLKLHALNSGKIWQ 960
            ELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETLKLHALNSGKIWQ
Sbjct: 901  ELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETLKLHALNSGKIWQ 960

Query: 961  DQLPASFSGVKNLTSLSVEGCASIKYLMTITMAKSLVHLERLEISNCKLMKDITISEARD 1020
            DQLPASFSGVKNLTSLSVEGCASIKYL TITMAKSLVHLERLEISNCKLMKDITISEARD
Sbjct: 961  DQLPASFSGVKNLTSLSVEGCASIKYLTTITMAKSLVHLERLEISNCKLMKDITISEARD 1020

Query: 1021 LGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLKKVDIRHCRRLET 1080
            LGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLKKVDIRHCRRLET
Sbjct: 1021 LGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLKKVDIRHCRRLET 1080

Query: 1081 IFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHLKELKLLRLPKLK 1140
            IFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHLKELKLLRLPKLK
Sbjct: 1081 IFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHLKELKLLRLPKLK 1140

Query: 1141 HIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQFCGVEEIVSKGG 1200
            HIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQFCGVEEIVSKGG
Sbjct: 1141 HIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQFCGVEEIVSKGG 1200

Query: 1201 GEDEDAAFVLTGLISLHLWNLFEFKRFYPGKYTLDCPSLQLLDVRHCKSFKLLEGALEKL 1260
            GEDED AF+LTGL SLHLWNLFEFKRFYPGKYTLDCPSLQLLDVRHCKSFKLLEGALEK 
Sbjct: 1201 GEDEDVAFLLTGLTSLHLWNLFEFKRFYPGKYTLDCPSLQLLDVRHCKSFKLLEGALEKS 1260

Query: 1261 SPPADDKVEDDQSSKKEQLERRESDVLHEMSTSKKEERTTTTAQEDKVEAELTEIRAQLQ 1320
            SPPAD+KVEDDQSSKKEQLERRESDVL+EMSTSKKEERTTTTAQED VEAELTEIRAQLQ
Sbjct: 1261 SPPADEKVEDDQSSKKEQLERRESDVLNEMSTSKKEERTTTTAQEDTVEAELTEIRAQLQ 1320

Query: 1321 TLVMGQNQMMEHLAQLTAIARQTASR 1347
            TLVMGQNQMMEHLAQLTAIARQTASR
Sbjct: 1321 TLVMGQNQMMEHLAQLTAIARQTASR 1346

BLAST of MS021966 vs. ExPASy TrEMBL
Match: A0A516F3L5 (Disease resistance protein PRS2 OS=Cucurbita ficifolia OX=37645 GN=RFN2 PE=2 SV=1)

HSP 1 Score: 2136.3 bits (5534), Expect = 0.0e+00
Identity = 1113/1367 (81.42%), Postives = 1217/1367 (89.03%), Query Frame = 0

Query: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
            MEICS AIVGPIGE+ITNC VDPVFRQLDYL+HFRSNV NL DQ KKLVETRDFVQH+VD
Sbjct: 1    MEICSAAIVGPIGEKITNCAVDPVFRQLDYLVHFRSNVNNLSDQRKKLVETRDFVQHAVD 60

Query: 61   SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
            SAKSNGDEIEGMVVEWLS  +KFS++VDRFFNEANGRSLRWWNM SRHRFSRRA K+A+E
Sbjct: 61   SAKSNGDEIEGMVVEWLSITKKFSDEVDRFFNEANGRSLRWWNMLSRHRFSRRATKMAME 120

Query: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180
            VVKAIEEGRFERVAYR+PP GI  + NSKKFEAFESRV I KEIIEALADA ASVIVVHG
Sbjct: 121  VVKAIEEGRFERVAYRIPPQGIMMLNNSKKFEAFESRVLILKEIIEALADANASVIVVHG 180

Query: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240
            MGGVGKTTL EE+ARL IE KLFDA+AM TVTQ P+VKRIQGEIADQLGLKFEEE +RVR
Sbjct: 181  MGGVGKTTLVEEIARLVIEGKLFDALAMTTVTQIPNVKRIQGEIADQLGLKFEEEKDRVR 240

Query: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300
            A+RLRRRLEMEKKVLVILDDVWAKLDLE +G S HHKGCKILVTSRKDDL+F DFGTQKN
Sbjct: 241  ADRLRRRLEMEKKVLVILDDVWAKLDLEDVGISSHHKGCKILVTSRKDDLYFGDFGTQKN 300

Query: 301  IKINVLSKKEARNFFNKMACDFVD-YNDSDPEMEAVATELADECAGLPLALATVAQALKG 360
            IKI+VL+KKEARNFFNKMACDFV+  NDSDPEMEAVATELADECAGLPLALATVAQALKG
Sbjct: 301  IKIDVLAKKEARNFFNKMACDFVESSNDSDPEMEAVATELADECAGLPLALATVAQALKG 360

Query: 361  KGRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQI 420
            KGRPIWNDAL ELKFP QPNNYGVNKE YSSLKLSYKSL+RDEARSLFLLCSLFP+DYQI
Sbjct: 361  KGRPIWNDALHELKFPSQPNNYGVNKEAYSSLKLSYKSLNRDEARSLFLLCSLFPQDYQI 420

Query: 421  NIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA 480
            NIKYLLMYAMGLGLLNGM+SL  A+ RILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA
Sbjct: 421  NIKYLLMYAMGLGLLNGMNSLAAAKWRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA 480

Query: 481  ILVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGK 540
            IL+ASKMKSKYLVRHG GE +WP +DEFKDYTAISLGCSD +ELPEF CPQL+FLLLVGK
Sbjct: 481  ILIASKMKSKYLVRHGSGESLWPSMDEFKDYTAISLGCSDHSELPEFICPQLRFLLLVGK 540

Query: 541  RTPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELK 600
            RT LRLPE FFAGMQELRVLDLT LCI +LPSSI++LVNL TLCLDDCVLPDMSIVGELK
Sbjct: 541  RTSLRLPEKFFAGMQELRVLDLTSLCIQRLPSSIDELVNLHTLCLDDCVLPDMSIVGELK 600

Query: 601  KLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFK 660
            KLEILSLRASDI+ALPRVIG+LTNLK+LNLSNC KLKVIPANLLS+LTGL ELY+DNSFK
Sbjct: 601  KLEILSLRASDIIALPRVIGELTNLKVLNLSNCFKLKVIPANLLSKLTGLSELYMDNSFK 660

Query: 661  LWNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDW 720
             WNVGH NEGNINA I+ELNNLPRLTTLHVHI +  +LP AS+F NL GYRILIGDGWDW
Sbjct: 661  HWNVGHMNEGNINARISELNNLPRLTTLHVHIPNPTLLPNASVFGNLSGYRILIGDGWDW 720

Query: 721  SGKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDEL--RSAKNILFSLDHKGFPKLK 780
            SGK+ETSKTLKLKLE   QREDAIQALLENTE+LYLDEL   SA++ILFSLDHKGFPKLK
Sbjct: 721  SGKFETSKTLKLKLERGIQREDAIQALLENTENLYLDELGSASARDILFSLDHKGFPKLK 780

Query: 781  RLRVHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVK 840
             LRV NN+EIV+VVNS++MQH HSAFPLLESL LKNL  LG ICRG LPQMSFRNLK VK
Sbjct: 781  CLRVKNNSEIVSVVNSDSMQHLHSAFPLLESLFLKNLDELGSICRGRLPQMSFRNLKIVK 840

Query: 841  VESCSRLKFVFPPSMG-GGLV-LQKLEISECPVMETVVS--------IHDDKMDEKNMIE 900
            VESC+RLKFVFP  MG GGL+ LQKLEISEC  MET+VS        I+ DK DEKN IE
Sbjct: 841  VESCNRLKFVFPSFMGRGGLIHLQKLEISECRAMETIVSKGIETQMQINGDKWDEKNKIE 900

Query: 901  FPELRSLILQHLPELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLET 960
            FPELRSLILQHLPELM FYC++ S AVPSTK D+   IS +K  FHPLLN++VSFPKLET
Sbjct: 901  FPELRSLILQHLPELMSFYCYD-SLAVPSTKRDSDQIISAIKTGFHPLLNQQVSFPKLET 960

Query: 961  LKLHALNSGKIWQDQLPASFSGVKNLTSLSVEGCASIKYLMTITMAKSLVHLERLEISNC 1020
            LKLHALN+GKIWQDQ+P+SFSGVKNLTSLSV  CASIKYLMTIT+A+SLV+LERLEI+NC
Sbjct: 961  LKLHALNAGKIWQDQVPSSFSGVKNLTSLSVVDCASIKYLMTITVAQSLVNLERLEINNC 1020

Query: 1021 KLMKDITISEARDLGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKL 1080
            K+M+ I ISE +DL +++ ++SI QN+NVFPSLESLVISRMD+LETLWDKEATSG+F+KL
Sbjct: 1021 KMMQAIIISEDKDLDNNHPSKSILQNQNVFPSLESLVISRMDALETLWDKEATSGSFTKL 1080

Query: 1081 KKVDIRHCRRLETIFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANH 1140
            KKVDI  C++LET+FP+YMLNRLTNLERL VTDCSS+VEIFQVK  V NG QVAA+AANH
Sbjct: 1081 KKVDIGQCKKLETVFPNYMLNRLTNLERLNVTDCSSVVEIFQVKVPVINGKQVAAIAANH 1140

Query: 1141 LKELKLLRLPKLKHIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKI 1200
            LKELKLLRLPKLKHIWS+DP RFLSYPSLQ+VHTIHCQSL+N+FP SIAKDL QLEELKI
Sbjct: 1141 LKELKLLRLPKLKHIWSTDPQRFLSYPSLQLVHTIHCQSLLNIFPVSIAKDLFQLEELKI 1200

Query: 1201 QFCGVEEIVSKGGGED-----EDAAFVLTGLISLHLWNLFEFKRFYPGKYTLDCPSLQLL 1260
            QFCGVEEIV+KGG E      EDA+FVL+GL SL LWNLFEFKRFYPGKYTLDCPSL LL
Sbjct: 1201 QFCGVEEIVAKGGAEGDGDEYEDASFVLSGLTSLSLWNLFEFKRFYPGKYTLDCPSLTLL 1260

Query: 1261 DVRHCKSFKLLEGALEKLS-PPADDKVEDDQSSKKEQLERRE--SDVLHEMSTSKKEERT 1320
            DVRHCKSFKL+EG  E  +   A +KVEDDQS+++ ++ERRE  +DVL E ST +KEE T
Sbjct: 1261 DVRHCKSFKLMEGTSENSTLSSALEKVEDDQSTQRGKIERREADADVLKETST-RKEEIT 1320

Query: 1321 TTTAQEDKVEAELTEIRAQLQTLVMGQNQMMEHLAQLTAIARQTASR 1347
            TT   +D VEAEL EIRAQLQ LV+GQNQMMEHLAQLT  A QT S+
Sbjct: 1321 TTV--QDNVEAELIEIRAQLQALVVGQNQMMEHLAQLTTTAHQTISK 1363

BLAST of MS021966 vs. ExPASy TrEMBL
Match: A0A6J1K5D0 (disease resistance protein RPS2-like OS=Cucurbita maxima OX=3661 GN=LOC111492444 PE=4 SV=1)

HSP 1 Score: 2125.9 bits (5507), Expect = 0.0e+00
Identity = 1099/1364 (80.57%), Postives = 1210/1364 (88.71%), Query Frame = 0

Query: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
            MEICS AIVGPIGE+ITNC VDPVFRQLDYL+HFRSNV NL DQ KKLVETRDFVQH+VD
Sbjct: 1    MEICSAAIVGPIGEKITNCTVDPVFRQLDYLVHFRSNVNNLSDQRKKLVETRDFVQHAVD 60

Query: 61   SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
            SAKSNGD+IEGMVVEWLS  +KF ++VD FFNEANGRSLRWWNM SRHRFSRRA K+A E
Sbjct: 61   SAKSNGDQIEGMVVEWLSITKKFCDEVDSFFNEANGRSLRWWNMLSRHRFSRRATKMATE 120

Query: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180
            VVK IEEGRFERVAYR+PP GI  + NSKKFEAFESR+ I KEIIEALADA ASVIVVHG
Sbjct: 121  VVKTIEEGRFERVAYRIPPQGIMMLNNSKKFEAFESRILILKEIIEALADANASVIVVHG 180

Query: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240
            MGGVGKTTL EE+ARL IE KLFDA+AM TVTQ P+VKRIQGEIADQLGLKFEEE +RVR
Sbjct: 181  MGGVGKTTLVEEIARLVIEGKLFDALAMTTVTQIPNVKRIQGEIADQLGLKFEEEKDRVR 240

Query: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300
            A+RLRRRLEMEKKVLVILDDVWA LDLE IG S HHKGCKILVTSRKDDL+F DFGTQKN
Sbjct: 241  ADRLRRRLEMEKKVLVILDDVWANLDLEDIGISSHHKGCKILVTSRKDDLYFGDFGTQKN 300

Query: 301  IKINVLSKKEARNFFNKMACDFVD-YNDSDPEMEAVATELADECAGLPLALATVAQALKG 360
            IKI+VL+KKEARNFFNKMACDFV+  NDSDPEMEAVATELADECAGLPLALATVAQALKG
Sbjct: 301  IKIDVLAKKEARNFFNKMACDFVESSNDSDPEMEAVATELADECAGLPLALATVAQALKG 360

Query: 361  KGRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQI 420
            KGRPIWNDAL ELKFP QPNNYGVNKE YSSLKLSYKSL+RDEARSLFLLCSLFP+DYQI
Sbjct: 361  KGRPIWNDALHELKFPSQPNNYGVNKEAYSSLKLSYKSLNRDEARSLFLLCSLFPQDYQI 420

Query: 421  NIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA 480
            NIKYLLMYAMGLGLLNGM+SL  A+ RILSLVDELKTSYLLLDG DSDFVKMHDIVRDTA
Sbjct: 421  NIKYLLMYAMGLGLLNGMNSLAAAKWRILSLVDELKTSYLLLDGVDSDFVKMHDIVRDTA 480

Query: 481  ILVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGK 540
            IL+ASKMKSKYLVRHG GE +WPP+DEFKDYTAISLGCSD +ELPEF CPQL+FLLLVGK
Sbjct: 481  ILIASKMKSKYLVRHGAGESLWPPMDEFKDYTAISLGCSDHSELPEFICPQLRFLLLVGK 540

Query: 541  RTPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELK 600
            RT LRLPE FFAGMQELRVLDLT LCI QLPSSI+QLVNL TLCLDDCVLPDMSIVGELK
Sbjct: 541  RTSLRLPEKFFAGMQELRVLDLTSLCIQQLPSSIDQLVNLHTLCLDDCVLPDMSIVGELK 600

Query: 601  KLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFK 660
            KLEILSLRASDI+ALPRVIG+LTNLK+LNLSNC KLKVIPANLLS+LTGL ELY+DNSFK
Sbjct: 601  KLEILSLRASDIIALPRVIGELTNLKVLNLSNCFKLKVIPANLLSKLTGLSELYMDNSFK 660

Query: 661  LWNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDW 720
             WNVGH NEGNINA I+ELN LPRLTTLHVHI +  +LP AS+F NL GYRILIGDGWDW
Sbjct: 661  HWNVGHMNEGNINARISELNYLPRLTTLHVHIPNPILLPNASVFGNLTGYRILIGDGWDW 720

Query: 721  SGKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDEL--RSAKNILFSLDHKGFPKLK 780
            SGK+ETSKTLKLKLE   QREDAIQALLENTE+LYLDEL   SA++IL+SLDHKGFPKLK
Sbjct: 721  SGKFETSKTLKLKLERGNQREDAIQALLENTENLYLDELGSASARDILYSLDHKGFPKLK 780

Query: 781  RLRVHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVK 840
             LRV NN+EIV+VVNS++MQH HSAFPLLESL LKNL  LG ICRG LPQMSFRNLK VK
Sbjct: 781  CLRVKNNSEIVSVVNSDSMQHLHSAFPLLESLFLKNLDELGSICRGRLPQMSFRNLKIVK 840

Query: 841  VESCSRLKFVFPPSMGGGLV-LQKLEISECPVMETVVS--------IHDDKMDEKNMIEF 900
            VESC+RLKFVFP  MG GL+ LQKLEISEC  MET+VS        I+ DK  EKN IEF
Sbjct: 841  VESCNRLKFVFPSFMGRGLIHLQKLEISECRAMETIVSKGIETQMQINGDKWVEKNKIEF 900

Query: 901  PELRSLILQHLPELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETL 960
            PELRSLILQHLPELM FYC++ S AVPSTK D+   IS +K  FHPLLN++VSFPKLETL
Sbjct: 901  PELRSLILQHLPELMSFYCYD-SLAVPSTKRDSDQIISAIKTGFHPLLNQQVSFPKLETL 960

Query: 961  KLHALNSGKIWQDQLPASFSGVKNLTSLSVEGCASIKYLMTITMAKSLVHLERLEISNCK 1020
            KLHALN+GKIWQDQ+P+SFSGVKNLTSLSV  CASIKYLMTIT+A+SLV+LERLEI+NCK
Sbjct: 961  KLHALNAGKIWQDQVPSSFSGVKNLTSLSVVDCASIKYLMTITVAQSLVNLERLEINNCK 1020

Query: 1021 LMKDITISEARDLGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLK 1080
            +M+ I ISE +DL +++ ++SI QN+NVFPSLESLV+SRMD+LETLWDKEATSG+FSKLK
Sbjct: 1021 MMQAIIISEDKDLDNNHPSKSILQNQNVFPSLESLVVSRMDALETLWDKEATSGSFSKLK 1080

Query: 1081 KVDIRHCRRLETIFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHL 1140
            KVDIRHC++LET+FP++MLNRLTNLERL V DCSS+VEIF+VK  V NG QVAA+AANHL
Sbjct: 1081 KVDIRHCKKLETVFPNFMLNRLTNLERLNVIDCSSVVEIFEVKVPVINGKQVAAIAANHL 1140

Query: 1141 KELKLLRLPKLKHIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQ 1200
            KELKLLRLPKLKHIWS+DP RFL+YPSLQ+VHTIHC+SL+N+FP SIAKDL QLEELKIQ
Sbjct: 1141 KELKLLRLPKLKHIWSTDPQRFLNYPSLQLVHTIHCRSLLNIFPLSIAKDLFQLEELKIQ 1200

Query: 1201 FCGVEEIVSKGGGED-----EDAAFVLTGLISLHLWNLFEFKRFYPGKYTLDCPSLQLLD 1260
            FCGVEEIV+KGG E      EDA+FVL+GL SL LWNLFEFKRFYPGKYTLDCPSL LLD
Sbjct: 1201 FCGVEEIVAKGGAEGDGDEYEDASFVLSGLTSLSLWNLFEFKRFYPGKYTLDCPSLTLLD 1260

Query: 1261 VRHCKSFKLLEGALEKLS-PPADDKVEDDQSSKKEQLERRESDVLHEMSTSKKEERTTTT 1320
            VRHCKSFKL+EG  E  +   A +K EDD+S+++ ++ERRE+DVL E + ++KEE TTT 
Sbjct: 1261 VRHCKSFKLMEGTSESSTLSSALEKAEDDESTQRGKIERREADVLKEETFTRKEEITTTV 1320

Query: 1321 AQEDKVEAELTEIRAQLQTLVMGQNQMMEHLAQLTAIARQTASR 1347
              +D VEAEL EIRAQLQ LV+GQNQMMEHLAQLT IAR+  S+
Sbjct: 1321 --QDNVEAELIEIRAQLQALVVGQNQMMEHLAQLTTIAREGISK 1361

BLAST of MS021966 vs. ExPASy TrEMBL
Match: A0A6J1EPC8 (disease resistance protein RPS2-like OS=Cucurbita moschata OX=3662 GN=LOC111436165 PE=4 SV=1)

HSP 1 Score: 2119.4 bits (5490), Expect = 0.0e+00
Identity = 1099/1364 (80.57%), Postives = 1209/1364 (88.64%), Query Frame = 0

Query: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
            MEICS AIVGPIGE+ITNC VDPVFRQLDYL+HFRSNV NL DQ KKLVETRDFVQH+VD
Sbjct: 1    MEICSAAIVGPIGEKITNCTVDPVFRQLDYLVHFRSNVNNLSDQRKKLVETRDFVQHAVD 60

Query: 61   SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
            SAKSNGDEIEGMVVEWLS  ++FS++VDRFFNEAN RSLRWWNM SRHRFSRRA K+A+E
Sbjct: 61   SAKSNGDEIEGMVVEWLSITKQFSDEVDRFFNEANVRSLRWWNMLSRHRFSRRATKMAME 120

Query: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180
            VVKAIEEGRFERVAYR+PP GI  + NSKKFEAFESRV I KEIIEALADA ASVIVVHG
Sbjct: 121  VVKAIEEGRFERVAYRIPPQGIMMLNNSKKFEAFESRVLILKEIIEALADANASVIVVHG 180

Query: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240
            MGGVGKTTL EE+ARL IE KLFDA+AM TVTQ P+VKRIQGEIADQLGLKFEEE +RVR
Sbjct: 181  MGGVGKTTLVEEIARLVIEGKLFDALAMTTVTQIPNVKRIQGEIADQLGLKFEEEKDRVR 240

Query: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300
            A+RLRRRLEMEKKVLVILDDVWAKLDLE +G S HHKGCKILVTSRKDDL+F DFGTQKN
Sbjct: 241  ADRLRRRLEMEKKVLVILDDVWAKLDLEDVGISSHHKGCKILVTSRKDDLYFGDFGTQKN 300

Query: 301  IKINVLSKKEARNFFNKMACDFVD-YNDSDPEMEAVATELADECAGLPLALATVAQALKG 360
            IKI+VL+KKEARNFFNKMACDFV+  NDSDPEMEAVATELADECAGLPLALATVAQALKG
Sbjct: 301  IKIDVLAKKEARNFFNKMACDFVESSNDSDPEMEAVATELADECAGLPLALATVAQALKG 360

Query: 361  KGRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQI 420
            KGRPIWNDAL ELKFP QPNNYGVNKE YSSLKLSYKSL+RDEARSLFLLCSLFP+DYQI
Sbjct: 361  KGRPIWNDALHELKFPSQPNNYGVNKEAYSSLKLSYKSLNRDEARSLFLLCSLFPQDYQI 420

Query: 421  NIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA 480
            NIKYLLMYAMGLGLLNGM+SL  A+ RILSLVDELKTSYLLLDG DSDFVKMHDIVRDTA
Sbjct: 421  NIKYLLMYAMGLGLLNGMNSLAAAKWRILSLVDELKTSYLLLDGVDSDFVKMHDIVRDTA 480

Query: 481  ILVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGK 540
            IL+ASKMKSKYLVRHG GE +WPP+DEFKDYTAISLGCSD TELPEF CPQL+FLLLVGK
Sbjct: 481  ILIASKMKSKYLVRHGAGESLWPPMDEFKDYTAISLGCSDHTELPEFICPQLRFLLLVGK 540

Query: 541  RTPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELK 600
            RT LRLPE FFAGMQELRVLDLT LCI QLPSSI+QLVNL TLCLDDCVLPDMSIVGELK
Sbjct: 541  RTSLRLPEKFFAGMQELRVLDLTSLCIQQLPSSIDQLVNLHTLCLDDCVLPDMSIVGELK 600

Query: 601  KLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFK 660
            KLEILSLRASDI+ALPRVIG+LTNLK+LNLSNC KLKVIPANLLS+LTGL ELY+DNSFK
Sbjct: 601  KLEILSLRASDIIALPRVIGELTNLKVLNLSNCFKLKVIPANLLSKLTGLSELYMDNSFK 660

Query: 661  LWNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDW 720
             WNVGH NEGNINA I+ELN LPRLTTLHVHI +  +LP AS+F NL GYRILIGDGWDW
Sbjct: 661  HWNVGHMNEGNINARISELNYLPRLTTLHVHIPNPTLLPNASVFGNLSGYRILIGDGWDW 720

Query: 721  SGKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDELRS--AKNILFSLDHKGFPKLK 780
            SGK+ETSKTLKLKLE   QREDAIQALLENTE+LYLDEL S  A++IL+SLDHKGFPKLK
Sbjct: 721  SGKFETSKTLKLKLERGIQREDAIQALLENTENLYLDELGSARARDILYSLDHKGFPKLK 780

Query: 781  RLRVHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVK 840
             LRV NN+EIV+VV S+ MQH HSAFPLLESL LKNL  L  ICRG LPQMSFRNLK VK
Sbjct: 781  CLRVKNNSEIVSVVKSDGMQHQHSAFPLLESLFLKNLDELASICRGRLPQMSFRNLKIVK 840

Query: 841  VESCSRLKFVFPPSMGGGLV-LQKLEISECPVMETVVS--------IHDDKMDEKNMIEF 900
            VESC+RLKFVFP  MG GL+ LQKLEISEC  MET+VS        I+ DK DEKN IEF
Sbjct: 841  VESCNRLKFVFPSFMGRGLMHLQKLEISECRAMETIVSKGNETQMQINGDKWDEKNKIEF 900

Query: 901  PELRSLILQHLPELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETL 960
            PELRSLILQHLPELM FY ++ S A+PSTK D+   IS  K  FHPLLN++VSFPKLETL
Sbjct: 901  PELRSLILQHLPELMSFYSYD-SLAMPSTKRDSDQIISATKTGFHPLLNQQVSFPKLETL 960

Query: 961  KLHALNSGKIWQDQLPASFSGVKNLTSLSVEGCASIKYLMTITMAKSLVHLERLEISNCK 1020
            KLH+LN+GKIWQDQ+P   SGVKNLTSLSV  CASIKYLMTIT+A+SLV+LERLEI+NCK
Sbjct: 961  KLHSLNAGKIWQDQVP---SGVKNLTSLSVVDCASIKYLMTITVAQSLVNLERLEINNCK 1020

Query: 1021 LMKDITISEARDLGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLK 1080
            +M+ I ISE +DL +++ ++SI QN+NVFPSLESLV+SRMD+LETLWDKEATSG+F+KLK
Sbjct: 1021 MMQAIIISEDKDLDNNHPSKSILQNQNVFPSLESLVVSRMDALETLWDKEATSGSFTKLK 1080

Query: 1081 KVDIRHCRRLETIFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHL 1140
            KVDIRHC++LET+FP+YMLNRLTNLERL VTDC S+VEIF+VK  V NG QVAA+AANHL
Sbjct: 1081 KVDIRHCKKLETVFPNYMLNRLTNLERLNVTDCRSVVEIFEVKVPVINGKQVAAIAANHL 1140

Query: 1141 KELKLLRLPKLKHIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQ 1200
            KELKLLRLPKLKHIWS+DP RFLSYPSLQ+VHTIHCQSL+N+FP SIAKDL QLEELKIQ
Sbjct: 1141 KELKLLRLPKLKHIWSTDPQRFLSYPSLQLVHTIHCQSLLNIFPVSIAKDLFQLEELKIQ 1200

Query: 1201 FCGVEEIVSKGGGED-----EDAAFVLTGLISLHLWNLFEFKRFYPGKYTLDCPSLQLLD 1260
            FCGVEEIV+KGG E      EDA+FVL+GL SL LWNLFEFKRFYPGKYTLDCPSL LLD
Sbjct: 1201 FCGVEEIVAKGGAEGDGDEYEDASFVLSGLTSLSLWNLFEFKRFYPGKYTLDCPSLTLLD 1260

Query: 1261 VRHCKSFKLLEGALEKLS-PPADDKVEDDQSSKKEQLERRESDVLHEMSTSKKEERTTTT 1320
            VRHCKSFKL+EG  E  +   A +KVEDD+S+++ ++ERRE+DVL E ++++KEE TTT 
Sbjct: 1261 VRHCKSFKLMEGTSESSTLSSALEKVEDDESTQRGKIERREADVLKEETSTRKEEITTTV 1320

Query: 1321 AQEDKVEAELTEIRAQLQTLVMGQNQMMEHLAQLTAIARQTASR 1347
              +D VEA+L EIRAQLQ LV+GQNQMMEHLAQLT IAR+  S+
Sbjct: 1321 --QDNVEAKLIEIRAQLQALVVGQNQMMEHLAQLTTIAREGISK 1358

BLAST of MS021966 vs. ExPASy TrEMBL
Match: A0A0A0LH57 (AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G075440 PE=4 SV=1)

HSP 1 Score: 2021.1 bits (5235), Expect = 0.0e+00
Identity = 1049/1361 (77.08%), Postives = 1178/1361 (86.55%), Query Frame = 0

Query: 1    MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
            ME+C+ AIV PI E+I NC VDPVFRQLDYLLHF++NV +L+DQ KKLVETRDFVQHSVD
Sbjct: 1    MELCAGAIVNPIAEKIANCTVDPVFRQLDYLLHFKTNVNDLKDQGKKLVETRDFVQHSVD 60

Query: 61   SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
            SAK+NG EIE MV EWL  A++FSEDVDRFFNEA+GRSLRWWNM SRHRFSRRA KLAV 
Sbjct: 61   SAKTNGYEIEVMVTEWLGIADQFSEDVDRFFNEADGRSLRWWNMLSRHRFSRRATKLAVA 120

Query: 121  VVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHG 180
            V KAI+ G FERV +R+ P  I T++N+KKFEAFESRV I KEIIEA+ DA A VIVVHG
Sbjct: 121  VDKAIQGGSFERVGFRVTPQEIMTLRNNKKFEAFESRVLILKEIIEAVGDANARVIVVHG 180

Query: 181  MGGVGKTTLAEEVARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVR 240
            M GVGKTTL EE+ARLA E KLFDA+AM TV   P++K+IQGEIADQLGLKFEEE ER+R
Sbjct: 181  MAGVGKTTLVEEIARLAKEGKLFDAIAMVTVKHIPNIKKIQGEIADQLGLKFEEEKERIR 240

Query: 241  ANRLRRRLEMEKKVLVILDDVWAKLDLEAIGTSGHHKGCKILVTSRKDDLFFDDFGTQKN 300
            A+RLRRRLEMEKKVLV+LDDVW++LDLEA+G S HHKGCKILVTSRKDDLFF+DFGTQKN
Sbjct: 241  ADRLRRRLEMEKKVLVVLDDVWSRLDLEAVGISSHHKGCKILVTSRKDDLFFNDFGTQKN 300

Query: 301  IKINVLSKKEARNFFNKMACDFVD-YNDSDPEMEAVATELADECAGLPLALATVAQALKG 360
            I IN+LSKKEAR+FFNK+ACD V+  +D+DPEMEAVATELADEC GLPL+LATV QALKG
Sbjct: 301  IYINILSKKEARDFFNKVACDSVESSDDTDPEMEAVATELADECGGLPLSLATVGQALKG 360

Query: 361  KGRPIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQI 420
            KG P WNDALQ +KFPG+P+NYGVNK  Y SLK+SY+SL+R+EARSLFLLCSLFPEDYQI
Sbjct: 361  KGLPSWNDALQGMKFPGEPSNYGVNKVAYLSLKVSYRSLNREEARSLFLLCSLFPEDYQI 420

Query: 421  NIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTA 480
            NIKYLLMYAMGLGLLN MSSL +A+ RILSLVDELKTS+LLLDG D+DFVKMHDIVRDTA
Sbjct: 421  NIKYLLMYAMGLGLLNAMSSLAMAKWRILSLVDELKTSHLLLDGVDNDFVKMHDIVRDTA 480

Query: 481  ILVASKMKSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTELPEFTCPQLKFLLLVGK 540
            IL+ASKMKSKYLVRHG GE +WPP+DEFKDYTAISLGCSD +ELPEF CPQL+FLLLVGK
Sbjct: 481  ILIASKMKSKYLVRHGAGESLWPPMDEFKDYTAISLGCSDHSELPEFICPQLRFLLLVGK 540

Query: 541  RTPLRLPESFFAGMQELRVLDLTGLCIVQLPSSINQLVNLQTLCLDDCVLPDMSIVGELK 600
            RT LRLPE FFAGMQELRVLDLTGLCI +LP SI+QLVNLQTLCLDDCVLPDMS+VGELK
Sbjct: 541  RTSLRLPEKFFAGMQELRVLDLTGLCIQRLPPSIDQLVNLQTLCLDDCVLPDMSVVGELK 600

Query: 601  KLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFK 660
            KLEILSLRASDI+ALPRVIG+LTNLKMLNLS+CSKLKVIPANLLSRL GL ELY+DNSFK
Sbjct: 601  KLEILSLRASDIIALPRVIGELTNLKMLNLSDCSKLKVIPANLLSRLIGLSELYMDNSFK 660

Query: 661  LWNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRASLFRNLIGYRILIGDGWDW 720
             WNVG   EG +NA I+EL+NLPRLTTLHVHI +  ILP A +FR L GYRILIGD WDW
Sbjct: 661  HWNVGQM-EGYVNARISELDNLPRLTTLHVHIPNPTILPHAFVFRKLSGYRILIGDRWDW 720

Query: 721  SGKYETSKTLKLKLESSFQREDAIQALLENTEDLYLDELRSAKNILFSLDHKGFPKLKRL 780
            SG YETS+TLKLKL+SS QREDAIQALLEN EDLYLDEL S KNILFSLD+KGFPKLK L
Sbjct: 721  SGNYETSRTLKLKLDSSIQREDAIQALLENIEDLYLDELESVKNILFSLDYKGFPKLKGL 780

Query: 781  RVHNNAEIVTVVNSENMQHPHSAFPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVKVE 840
            RV NN EIVTVVNS+NM HPHSAFPLLESL LKNLA LG ICRG LPQMSFRNLKRVKVE
Sbjct: 781  RVKNNGEIVTVVNSDNMHHPHSAFPLLESLFLKNLAELGSICRGKLPQMSFRNLKRVKVE 840

Query: 841  SCSRLKFVFPPSMGGGLV-LQKLEISECPVMETVVS--------IHDDKMDEKNMIEFPE 900
            SC RLKFVFP SM  GL+ LQ LEISEC ++ET+VS        I+ DK DE NMIEFPE
Sbjct: 841  SCDRLKFVFPSSMVRGLIHLQSLEISECGIIETIVSKNKETEMQINGDKWDE-NMIEFPE 900

Query: 901  LRSLILQHLPELMGFYCHESSTAVPSTKVDAGHTISTVKPSFHPLLNKEVSFPKLETLKL 960
            LRSLILQHLP LMGFYCH+  T VPSTKVD+  T+ T++PSFHPLL+++VSFPKLETLKL
Sbjct: 901  LRSLILQHLPALMGFYCHDCIT-VPSTKVDSRQTVFTIEPSFHPLLSQQVSFPKLETLKL 960

Query: 961  HALNSGKIWQDQLPASFSGVKNLTSLSVEGCASIKYLMTITMAKSLVHLERLEISNCKLM 1020
            HALNSGKIWQDQLP+SF G KNLTSLSVEGCASIKYLMTIT+A+SLV+LERLE+++CKLM
Sbjct: 961  HALNSGKIWQDQLPSSFYGFKNLTSLSVEGCASIKYLMTITVARSLVNLERLELNDCKLM 1020

Query: 1021 KDITISEARDLGSHYTTQSIFQNENVFPSLESLVISRMDSLETLWDKEATSGAFSKLKKV 1080
            K I ISE +DL ++Y ++SI QN++VF +LESL+ISRMD+LETLW  EA SG+F+KLKKV
Sbjct: 1021 KAIIISEDQDLDNNYPSKSILQNKDVFANLESLLISRMDALETLWVNEAASGSFTKLKKV 1080

Query: 1081 DIRHCRRLETIFPDYMLNRLTNLERLIVTDCSSLVEIFQVKGSVNNGDQVAAMAANHLKE 1140
            DIR+C++LETIFP+YMLNR+TNLERL VTDCSSLVEIFQVK  VNNG+QV  + ANHLKE
Sbjct: 1081 DIRNCKKLETIFPNYMLNRVTNLERLNVTDCSSLVEIFQVKVPVNNGNQVRDIGANHLKE 1140

Query: 1141 LKLLRLPKLKHIWSSDPHRFLSYPSLQVVHTIHCQSLVNLFPASIAKDLVQLEELKIQFC 1200
            LKLLRLPKLKHIWSSDPH FL YPSLQ+VHTIHCQSL+NLFP SIAKDL+QLE LKIQFC
Sbjct: 1141 LKLLRLPKLKHIWSSDPHNFLRYPSLQLVHTIHCQSLLNLFPVSIAKDLIQLEVLKIQFC 1200

Query: 1201 GVEEIVSKGGGE---DEDAAFVLTGLISLHLWNLFEFKRFYPGKYTLDCPSLQLLDVRHC 1260
            GVEEIV+K G +   D+ A+F+L+GL SL LWNLFEFKRFYPGKYTLDCPSL  LDVRHC
Sbjct: 1201 GVEEIVAKRGDDGDGDDAASFLLSGLTSLTLWNLFEFKRFYPGKYTLDCPSLTALDVRHC 1260

Query: 1261 KSFKLLEGALEKLS--PPADDKVEDDQSSKKEQLERRESDVLHEMSTSKKEERTTTTAQE 1320
            KSFKL+EG LE  S    A +KVE +QSS + + ERRES    E ST K+E    TT  +
Sbjct: 1261 KSFKLMEGTLENSSSISSAVEKVEVEQSSLRGEFERRES---KETSTGKEE---ITTIVQ 1320

Query: 1321 DKVEAELTEIRAQLQTLVMGQNQMMEHLAQLTAIARQTASR 1347
              V+AEL E+RAQL+ LV GQNQMME LAQLT I R+  S+
Sbjct: 1321 GVVDAELIELRAQLRALVAGQNQMMERLAQLTTIPREPVSK 1352

BLAST of MS021966 vs. TAIR 10
Match: AT4G27190.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 276.9 bits (707), Expect = 8.3e-74
Identity = 265/941 (28.16%), Postives = 442/941 (46.97%), Query Frame = 0

Query: 1   MEICSTAIVGPIGERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD 60
           ME C+     P+   I   M +  F ++   + F+SNV  L + L++L E +  +    +
Sbjct: 1   MECCA-----PVIGEILRLMYESTFSRVANAIKFKSNVKALNESLERLTELKGNMSEDHE 60

Query: 61  SAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWWNMPSRHRFSRRAVKLAVE 120
           +  +    +   ++ W   AE+          E          M  R R SR+ VK+ ++
Sbjct: 61  TLLTKDKPLRLKLMRWQREAEEVISKARLKLEERVS-----CGMSLRPRMSRKLVKI-LD 120

Query: 121 VVKAIEEGRFERV---AYRLPP------PGIFTVKNSKKFEAFESRVPIQKEIIEALADA 180
            VK +E+   E V   +    P      PG+  V  +           +  +I + L   
Sbjct: 121 EVKMLEKDGIEFVDMLSVESTPERVEHVPGVSVVHQTMASN-------MLAKIRDGLTSE 180

Query: 181 YASVIVVHGMGGVGKTTLAEEVARLAIE---CKLFDAVAMATVTQTPDVKRIQGEIADQL 240
            A  I V GMGGVGKTTL   +     E    + F  V    V++  D + +Q +IA++L
Sbjct: 181 KAQKIGVWGMGGVGKTTLVRTLNNKLREEGATQPFGLVIFVIVSKEFDPREVQKQIAERL 240

Query: 241 GLKFE-EESERVRANRLRRRLEMEKKVLVILDDVWAKLDLEAIG--TSGHHKGCKILVTS 300
            +  + EESE   A R+   L  E+K L+ILDDVW  +DL+ +G   +  +KG K+++TS
Sbjct: 241 DIDTQMEESEEKLARRIYVGLMKERKFLLILDDVWKPIDLDLLGIPRTEENKGSKVILTS 300

Query: 301 RKDDLFFDDFGTQKNIKINVLSKKEARNFFNKMACDFVDYNDSDPEMEAVATELADECAG 360
           R  ++      T  +++++ L +++A   F K A D V        +  +A  ++ EC G
Sbjct: 301 RFLEV-CRSMKTDLDVRVDCLLEEDAWELFCKNAGDVV----RSDHVRKIAKAVSQECGG 360

Query: 361 LPLALATVAQALKGKGR-PIWNDALQELKFPGQPNNYGVNKEVYSSLKLSYKSLDRDEAR 420
           LPLA+ TV  A++GK    +WN  L +L     P    + ++++  LKLSY  L+ D+A+
Sbjct: 361 LPLAIITVGTAMRGKKNVKLWNHVLSKLS-KSVPWIKSIEEKIFQPLKLSYDFLE-DKAK 420

Query: 421 SLFLLCSLFPEDYQINIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGA 480
             FLLC+LFPEDY I +  ++ Y M  G +  + S   +    ++ V+ LK   LL DG 
Sbjct: 421 FCFLLCALFPEDYSIEVTEVVRYWMAEGFMEELGSQEDSMNEGITTVESLKDYCLLEDGD 480

Query: 481 DSDFVKMHDIVRDTAILVASKMK--SKYLVRHGVG------EKMWPPIDEFKDYTAISLG 540
             D VKMHD+VRD AI + S  +  S  LV  G G      +K+ P +   +  + ++  
Sbjct: 481 RRDTVKMHDVVRDFAIWIMSSSQDDSHSLVMSGTGLQDIRQDKLAPSL---RRVSLMNNK 540

Query: 541 CSDFTELPEFTCPQLKFLLLVGKRTPLRLPESFFAGMQELRVLDLTGLCIVQLPS-SINQ 600
                +L E  C +   LLL G      +P  F      LR+L+L+G  I   PS S+ +
Sbjct: 541 LESLPDLVEEFCVKTSVLLLQGNFLLKEVPIGFLQAFPTLRILNLSGTRIKSFPSCSLLR 600

Query: 601 LVNLQTLCLDDCV-LPDMSIVGELKKLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSK 660
           L +L +L L DC  L  +  +  L KLE+L L  + I+  PR + +L   + L+LS    
Sbjct: 601 LFSLHSLFLRDCFKLVKLPSLETLAKLELLDLCGTHILEFPRGLEELKRFRHLDLSRTLH 660

Query: 661 LKVIPANLLSRLTGLCELYLDNSFKLWNV-GHANEGNINAGIAELNNLPRLTTLHV--HI 720
           L+ IPA ++SRL+ L  L + +S   W+V G   +G   A + E+  L RL  L +  H 
Sbjct: 661 LESIPARVVSRLSSLETLDMTSSHYRWSVQGETQKG--QATVEEIGCLQRLQVLSIRLHS 720

Query: 721 SDARILPRASLFRNLIGYRILIGDGWDWSGKYETSKTLKLKLESSFQREDAIQALLENTE 780
           S   +  R +  + L  +++++G  +    +++  +     L  S   + +I  LL  T 
Sbjct: 721 SPFLLNKRNTWIKRLKKFQLVVGSRYILRTRHDKRRLTISHLNVS---QVSIGWLLAYTT 780

Query: 781 DLYLDELRSAKNILFSL--DHKGFPKLKRLRVHNN-------AEIVTVVNSENMQHPHSA 840
            L L+  +  + ++  L  D+KGF  LK L + N         E+V+   S+        
Sbjct: 781 SLALNHCQGIEAMMKKLVSDNKGFKNLKSLTIENVIINTNSWVEMVSTNTSKQSSDILDL 840

Query: 841 FPLLESLSLKNLANLGLICRGTLPQMSFRNLKRVKVESCSRLKFVFPPSMGGGLVLQKLE 900
            P LE L L+ +         T   +    LK +++  C +L+ +        L +  LE
Sbjct: 841 LPNLEELHLRRVDLETFSELQTHLGLKLETLKIIEITMCRKLRTLL--DKRNFLTIPNLE 900

Query: 901 ISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLPELM 904
             E    +++ ++H+  +  +  +  P LR L L++LP L+
Sbjct: 901 EIEISYCDSLQNLHEALLYHQPFV--PNLRVLKLRNLPNLV 904

BLAST of MS021966 vs. TAIR 10
Match: AT4G27220.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 259.6 bits (662), Expect = 1.4e-68
Identity = 255/895 (28.49%), Postives = 411/895 (45.92%), Query Frame = 0

Query: 34  FRSNVTNLRDQLKKLVETRDFVQHSVDSAKSNGDEIEGMVVEWLSFAEKFSEDVDRFFNE 93
           FRSN   L   L++L   +  V  ++  +      +E  +  WL   E+ +  +     E
Sbjct: 2   FRSNARALNRALERLKNVQTKVNEALKRSGIQEKSLERKLRIWLRKVEE-NVPLGELILE 61

Query: 94  ANGRSLRWWNMPSRHRFSRRAVKLAVEVVKAIEEGR--FERVAYRLPPPGIFTVKNSKKF 153
                  W         S + V++  +V +  E+G+   ++++       I        F
Sbjct: 62  KRSSCAIW--------LSDKDVEILEKVKRLEEQGQDLIKKISVNKSSREIVERVLGPSF 121

Query: 154 EAFESRVPIQKEIIEALADAYASVIVVHGMGGVGKTTLAEEVARLAIE---CKLFDAVAM 213
              ++ + +  ++ + L       I V GMGGVGKTTL   +    ++    + F  V  
Sbjct: 122 HPQKTALEMLDKLKDCLKKKNVQKIGVWGMGGVGKTTLVRTLNNDLLKYAATQQFALVIW 181

Query: 214 ATVTQTPDVKRIQGEIADQLGLKFEEESERVRANRLRRRLEMEKKVLVILDDVWAKLDLE 273
            TV++  D+KR+Q +IA +LG +F  E        +  RL   K  L+ILDDVW  +DL+
Sbjct: 182 VTVSKDFDLKRVQMDIAKRLGKRFTREQMNQLGLTICERLIDLKNFLLILDDVWHPIDLD 241

Query: 274 AIG---TSGHHKGCKILVTSRKDDLFFDDFGTQKNIKINVLSKKEARNFFNKMACDFVDY 333
            +G        K  K+++TSR+ ++      T +NIK+  L +KEA   F    C  V  
Sbjct: 242 QLGIPLALERSKDSKVVLTSRRLEV-CQQMMTNENIKVACLQEKEAWELF----CHNVGE 301

Query: 334 NDSDPEMEAVATELADECAGLPLALATVAQALKGKGR-PIWNDALQELKFPGQPNNYGVN 393
             +   ++ +A +++ EC GLPLA+ T+ + L+GK +  +W   L  LK      +    
Sbjct: 302 VANSDNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLK--RSAPSIDTE 361

Query: 394 KEVYSSLKLSYKSLDRDEARSLFLLCSLFPEDYQINIKYLLMYAMGLGLLNGMSSLMVAR 453
           ++++ +LKLSY  L +D  +S FL C+LFPEDY I +  L+MY +  GLL+G        
Sbjct: 362 EKIFGTLKLSYDFL-QDNMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQHHYEDMM 421

Query: 454 GRILSLVDELKTSYLLLDGADSDFVKMHDIVRDTAILVASKMKSKY--LVRHGVGEKMWP 513
              ++LV+ LK S LL DG   D VKMHD+VRD AI   S     +  LV  G G   +P
Sbjct: 422 NEGVTLVERLKDSCLLEDGDSCDTVKMHDVVRDFAIWFMSSQGEGFHSLVMAGRGLIEFP 481

Query: 514 PIDEFKDYTAISLGCSDFTELPEFTCPQLK--FLLLVGKRTPLRLPESFFAGMQELRVLD 573
                     +SL  +    LP      ++   LLL G      +P  F      LR+LD
Sbjct: 482 QDKFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGNSHVKEVPNGFLQAFPNLRILD 541

Query: 574 LTGLCIVQLPSSINQLVNLQTLCLDDC-VLPDMSIVGELKKLEILSLRASDIVALPRVIG 633
           L+G+ I  LP S + L +L++L L +C  L ++  +  L KL+ L L  S I  LPR + 
Sbjct: 542 LSGVRIRTLPDSFSNLHSLRSLVLRNCKKLRNLPSLESLVKLQFLDLHESAIRELPRGLE 601

Query: 634 QLTNLKMLNLSNCSKLKVIPANLLSRLTGLCELYLDNSFKLWNV-GHANEGNINAGIAEL 693
            L++L+ + +SN  +L+ IPA  + +L+ L  L +  S   W + G   EG   A + E+
Sbjct: 602 ALSSLRYICVSNTYQLQSIPAGTILQLSSLEVLDMAGSAYSWGIKGEEREG--QATLDEV 661

Query: 694 NNLPRLTTLHVHISDARILPRA--SLFRNLIGYRILIGDGWDWS--GKYETSKTLKLKLE 753
             LP L  L + + D         SL + L  ++ L       S  G  E      L + 
Sbjct: 662 TCLPHLQFLAIKLLDVLSFSYEFDSLTKRLTKFQFLFSPIRSVSPPGTGEGC----LAIS 721

Query: 754 SSFQREDAIQALLENTEDL---YLDELRSAKNILFSLDHKGFPKLKRLRVHNNAEIVTVV 813
                  +I  LL++   L   Y + L      L +     F  +K L +H    +    
Sbjct: 722 DVNVSNASIGWLLQHVTSLDLNYCEGLNGMFENLVTKSKSSFVAMKALSIHYFPSLSLAS 781

Query: 814 NSENMQHPHSAFPLLESLSLK--NLANLGLICRGTLPQMSFRNLKRVKVESCSRLKFVFP 873
             E+       FP LE LSL   NL ++G +  G L  M  + LK ++V  C +LK +F 
Sbjct: 782 GCESQL---DLFPNLEELSLDNVNLESIGEL-NGFL-GMRLQKLKLLQVSGCRQLKRLFS 841

Query: 874 PSMGGGLV--LQKLEISECPVMETVVSIHDDKMDEKNMIEFPELRSLILQHLPEL 903
             +  G +  LQ++++  C  +E + +     +D       P+L  + L++LP+L
Sbjct: 842 DQILAGTLPNLQEIKVVSCLRLEELFNFSSVPVDFCAESLLPKLTVIKLKYLPQL 868

BLAST of MS021966 vs. TAIR 10
Match: AT1G12280.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 198.4 bits (503), Expect = 3.8e-50
Identity = 219/874 (25.06%), Postives = 402/874 (46.00%), Query Frame = 0

Query: 14  ERITNCMVDPVFRQLDYLLHFRSNVTNLRDQLKKLVETRDFVQHSVD-----SAKSNGDE 73
           + + N +   +   + Y+     NV  ++  ++ L + RD V+  VD       +    +
Sbjct: 12  DEVVNQISQGLCINVGYICELSKNVVAMKKDMEVLKKKRDDVKRRVDIEEFTRRRERLSQ 71

Query: 74  IEGMVVEWLSFAEKFSEDVDRFFNEANGRSLRWW-----NMPSRHRFSRRAVKLAVEVVK 133
           ++G +    +   KF+E +    N+A  + L  +     N+   + + +R V +  E+  
Sbjct: 72  VQGWLTNVSTVENKFNELLTT--NDAELQRLCLFGFCSKNVKMSYLYGKRVVLMLKEIES 131

Query: 134 AIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEIIEALADAYASVIVVHGMGG 193
              +G F+ V    P   I  ++          +  + + +   L +    ++ ++GMGG
Sbjct: 132 LSSQGDFDTVTLATP---IARIEEMPIQPTIVGQETMLERVWTRLTEDGDEIVGLYGMGG 191

Query: 194 VGKTTLAEEV-ARLAIECKLFDAVAMATVTQTPDVKRIQGEIADQLGLKFEEESERVRAN 253
           VGKTTL   +  + + +C  F  V    V+++PD+ RIQG+I  +L L  EE       N
Sbjct: 192 VGKTTLLTRINNKFSEKCSGFGVVIWVVVSKSPDIHRIQGDIGKRLDLGGEEWD---NVN 251

Query: 254 RLRRRLEM-----EKKVLVILDDVWAKLDLEAIGT--SGHHKGCKILVTSRKDDLFFDDF 313
             +R L++     ++K +++LDD+W K++LE +G        GCK++ T+R  D+     
Sbjct: 252 ENQRALDIYNVLGKQKFVLLLDDIWEKVNLEVLGVPYPSRQNGCKVVFTTRSRDV-CGRM 311

Query: 314 GTQKNIKINVLSKKEARNFFNKMACDFVDYNDSDPEMEAVATELADECAGLPLALATVAQ 373
                ++++ L   EA   F     +  +     P++  +A ++A +C GLPLAL  + +
Sbjct: 312 RVDDPMEVSCLEPNEAWELFQMKVGE--NTLKGHPDIPELARKVAGKCCGLPLALNVIGE 371

Query: 374 ALKGKGR-PIWNDALQEL-----KFPGQPNNYGVNKEVYSSLKLSYKSLDRDEARSLFLL 433
            +  K     W +A+  L     +FPG        +++   LK SY +L++++ +  FL 
Sbjct: 372 TMACKRMVQEWRNAIDVLSSYAAEFPGM-------EQILPILKYSYDNLNKEQVKPCFLY 431

Query: 434 CSLFPEDYQINIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDELKTSYLLLDGA-DSDF 493
           CSLFPEDY++  + L+ Y +  G ++   S   A  +   ++  L  + LLL+ A + + 
Sbjct: 432 CSLFPEDYRMEKERLIDYWICEGFIDENESRERALSQGYEIIGILVRACLLLEEAINKEQ 491

Query: 494 VKMHDIVRDTAILVASKM---KSKYLVRHGVGEKMWPPIDEFKDYTAISLGCSDFTEL-- 553
           VKMHD+VR+ A+ +AS +   K + +V+ GVG +  P +  +     +SL  ++   L  
Sbjct: 492 VKMHDVVREMALWIASDLGEHKERCIVQVGVGLREVPKVKNWSSVRRMSLMENEIEILSG 551

Query: 554 -PEFTCPQLKFLLLVGKRTPLRLPESFFAGMQELRVLDLTG-LCIVQLPSSINQLVNLQT 613
            PE  C +L  L L    + L + + FF  +  L VLDL+G   + +LP+ I++LV+L+ 
Sbjct: 552 SPE--CLELTTLFLQKNDSLLHISDEFFRCIPMLVVLDLSGNSSLRKLPNQISKLVSLRY 611

Query: 614 LCLDDCVLPDMSI-VGELKKLEILSLRASDIVALPRVIGQLTNLKMLNLSNCSKLKVIPA 673
           L L    +  + + + ELKKL  L L   D +   + I  ++     N+S+  KL+++ +
Sbjct: 612 LDLSWTYIKRLPVGLQELKKLRYLRL---DYMKRLKSISGIS-----NISSLRKLQLLQS 671

Query: 674 NLLSRLTGLCELYLDNSFKLWNVGHANEGNINAGIAELNNLPRLTTLHVHISDARILPRA 733
            +   ++ + EL L    ++ N+   +    +  + +L N PRL      +   +IL   
Sbjct: 672 KMSLDMSLVEELQLLEHLEVLNISIKS----SLVVEKLLNAPRL------VKCLQILVLR 731

Query: 734 SLFRNLIGYRILIGDGWDWSGKYETSK--TLKLKLES-----SFQREDAIQALLENTEDL 793
            +     G  +L     D   K    K    ++K+E      S  R    Q  L N   +
Sbjct: 732 GVQEESSG--VLTLPDMDNLNKVIIRKCGMCEIKIERKTLSLSSNRSPKTQ-FLHNLSTV 791

Query: 794 YLDELRSAKNILFSLDHKGFPKLKRLRVHNNAEIVTVVNSENMQHPHSAFPL--LESLSL 846
           ++      K++ + L     P L  L V ++  +  ++N E         P   LESL L
Sbjct: 792 HISSCDGLKDLTWLL---FAPNLTSLEVLDSELVEGIINQEKAMTMSGIIPFQKLESLRL 838

BLAST of MS021966 vs. TAIR 10
Match: AT1G61180.2 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 194.9 bits (494), Expect = 4.2e-49
Identity = 225/812 (27.71%), Postives = 374/812 (46.06%), Query Frame = 0

Query: 106 SRHRFSRRAVKLAVEVVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEII 165
           S +++ ++   L  EV K   EG F+ V+   PPP       S+  E        Q++++
Sbjct: 109 SSYKYGKKVFLLLEEVKKLNSEGNFDEVSQ--PPP------RSEVEERPTQPTIGQEDML 168

Query: 166 E----ALADAYASVIVVHGMGGVGKTTLAEEVARLAIEC-KLFDAVAMATVTQTPDVKRI 225
           E     L +    ++ +HGMGGVGKTTL +++     E    FD V    V++   + ++
Sbjct: 169 EKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAEIGGTFDIVIWIVVSKGVMISKL 228

Query: 226 QGEIADQLGL---KFEEESERVRANRLRRRLEMEKKVLVILDDVWAKLDLEAIGT--SGH 285
           Q +IA++L L    ++ ++E  +A  + R L+  K+ +++LDD+W K+DLEAIG      
Sbjct: 229 QEDIAEKLHLCDDLWKNKNESDKATDIHRVLK-GKRFVLMLDDIWEKVDLEAIGIPYPSE 288

Query: 286 HKGCKILVTSRKDDLFFDDFGTQKNIKINVLSKKEARNFFNKMACDFVDYNDSDPEMEAV 345
              CK+  T+R  ++   + G  K +++N L  ++A   F     D  +   SDP +  +
Sbjct: 289 VNKCKVAFTTRSREV-CGEMGDHKPMQVNCLEPEDAWELFKNKVGD--NTLSSDPVIVEL 348

Query: 346 ATELADECAGLPLALATVAQALKGKGR-PIWNDALQELKFPGQPNNYGVNKEVYSSLKLS 405
           A E+A +C GLPLAL  + + +  K     W  A+          +   NK +   LK S
Sbjct: 349 AREVAQKCRGLPLALNVIGETMSSKTMVQEWEHAIHVFNTSAAEFSDMQNK-ILPILKYS 408

Query: 406 YKSLDRDEARSLFLLCSLFPEDYQINIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDEL 465
           Y SL  +  +S FL C+LFPED +I  + L+ Y +  G +     +  AR +  +++  L
Sbjct: 409 YDSLGDEHIKSCFLYCALFPEDGEIYNEKLIDYWICEGFIGEDQVIKRARNKGYAMLGTL 468

Query: 466 KTSYLLLDGADSDFVKMHDIVRDTAILVAS---KMKSKYLVRHGVGEKMWPPIDEFKDYT 525
            T   LL    + +  MHD+VR+ A+ +AS   K K  ++V+ GVG    P + ++    
Sbjct: 469 -TRANLLTKVGTYYCVMHDVVREMALWIASDFGKQKENFVVQAGVGLHEIPKVKDWGAVR 528

Query: 526 AISLGCSDFTELP-EFTCPQLKFLLLVGKRTPLRLPESFFAGMQELRVLDLT-GLCIVQL 585
            +SL  +D  E+  E  C +L  L L   +    LP +F   MQ+L VLDL+      +L
Sbjct: 529 KMSLMDNDIEEITCESKCSELTTLFLQSNKLK-NLPGAFIRYMQKLVVLDLSYNRDFNKL 588

Query: 586 PSSINQLVNLQTLCLDDCVLPDMSI-VGELKKLEILSLRASDIVALPRVIGQLTNLKMLN 645
           P  I+ LV+LQ L L +  +  M I + ELKKL  L L  +D +     I +L +L++L 
Sbjct: 589 PEQISGLVSLQFLDLSNTSIEHMPIGLKELKKLTFLDLTYTDRLCSISGISRLLSLRLLR 648

Query: 646 LSNCSKLKVIPANL--LSRLTGLCELYLDNSFKLWNVGHANEGNINAGIAELNNLPRLTT 705
           L   SK+    + L  L +L  L EL +  S +L ++              L  L     
Sbjct: 649 LLG-SKVHGDASVLKELQQLQNLQELAITVSAELISLDQ-----------RLAKLISNLC 708

Query: 706 LHVHISDARILPRASLFRNLIGYRILIGDGWDWSGKYETSKTLKLKLESSFQREDAIQAL 765
           +   +     L   +   NL   R+        +  +   K  + + ESS+ R +     
Sbjct: 709 IEGFLQKPFDLSFLASMENLSSLRV-------ENSYFSEIKCRESETESSYLRINPKIPC 768

Query: 766 LENTEDLYLDELRSAKNILFSLDHKGFPKLKRLRVHNNAEIVTVVNSENMQHPHSAFPL- 825
             N   L + +  S K++ + L     P L  L + ++ E+  ++N E   +  S  P  
Sbjct: 769 FTNLSRLEIMKCHSMKDLTWIL---FAPNLVVLLIEDSREVGEIINKEKATNLTSITPFL 828

Query: 826 -LESLSLKNLANLGLICRGTLPQMSFRNLKRVKVESCSRLKFVFPPSMGGGLVLQKLEIS 885
            LE L L NL  L  I    LP   F  L  + V +C +L+   P +      +++ EI 
Sbjct: 829 KLEWLILYNLPKLESIYWSPLP---FPVLLTMDVSNCPKLR-KLPLNATSVSKVEEFEIH 878

Query: 886 ECPVMETVVSIHDDKMDEKNMIEFPELRSLIL 897
             P  E    +  +  D KN    P ++ +I+
Sbjct: 889 MYPPPEQENELEWEDDDTKNRF-LPSIKPVII 878

BLAST of MS021966 vs. TAIR 10
Match: AT1G61180.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 193.4 bits (490), Expect = 1.2e-48
Identity = 223/800 (27.88%), Postives = 368/800 (46.00%), Query Frame = 0

Query: 106 SRHRFSRRAVKLAVEVVKAIEEGRFERVAYRLPPPGIFTVKNSKKFEAFESRVPIQKEII 165
           S +++ ++   L  EV K   EG F+ V+   PPP       S+  E        Q++++
Sbjct: 109 SSYKYGKKVFLLLEEVKKLNSEGNFDEVSQ--PPP------RSEVEERPTQPTIGQEDML 168

Query: 166 E----ALADAYASVIVVHGMGGVGKTTLAEEVARLAIEC-KLFDAVAMATVTQTPDVKRI 225
           E     L +    ++ +HGMGGVGKTTL +++     E    FD V    V++   + ++
Sbjct: 169 EKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAEIGGTFDIVIWIVVSKGVMISKL 228

Query: 226 QGEIADQLGL---KFEEESERVRANRLRRRLEMEKKVLVILDDVWAKLDLEAIGT--SGH 285
           Q +IA++L L    ++ ++E  +A  + R L+  K+ +++LDD+W K+DLEAIG      
Sbjct: 229 QEDIAEKLHLCDDLWKNKNESDKATDIHRVLK-GKRFVLMLDDIWEKVDLEAIGIPYPSE 288

Query: 286 HKGCKILVTSRKDDLFFDDFGTQKNIKINVLSKKEARNFFNKMACDFVDYNDSDPEMEAV 345
              CK+  T+R  ++   + G  K +++N L  ++A   F     D  +   SDP +  +
Sbjct: 289 VNKCKVAFTTRSREV-CGEMGDHKPMQVNCLEPEDAWELFKNKVGD--NTLSSDPVIVEL 348

Query: 346 ATELADECAGLPLALATVAQALKGKGR-PIWNDALQELKFPGQPNNYGVNKEVYSSLKLS 405
           A E+A +C GLPLAL  + + +  K     W  A+          +   NK +   LK S
Sbjct: 349 AREVAQKCRGLPLALNVIGETMSSKTMVQEWEHAIHVFNTSAAEFSDMQNK-ILPILKYS 408

Query: 406 YKSLDRDEARSLFLLCSLFPEDYQINIKYLLMYAMGLGLLNGMSSLMVARGRILSLVDEL 465
           Y SL  +  +S FL C+LFPED +I  + L+ Y +  G +     +  AR +  +++  L
Sbjct: 409 YDSLGDEHIKSCFLYCALFPEDGEIYNEKLIDYWICEGFIGEDQVIKRARNKGYAMLGTL 468

Query: 466 KTSYLLLDGADSDFVKMHDIVRDTAILVAS---KMKSKYLVRHGVGEKMWPPIDEFKDYT 525
            T   LL    + +  MHD+VR+ A+ +AS   K K  ++V+ GVG    P + ++    
Sbjct: 469 -TRANLLTKVGTYYCVMHDVVREMALWIASDFGKQKENFVVQAGVGLHEIPKVKDWGAVR 528

Query: 526 AISLGCSDFTELP-EFTCPQLKFLLLVGKRTPLRLPESFFAGMQELRVLDLT-GLCIVQL 585
            +SL  +D  E+  E  C +L  L L   +    LP +F   MQ+L VLDL+      +L
Sbjct: 529 KMSLMDNDIEEITCESKCSELTTLFLQSNKLK-NLPGAFIRYMQKLVVLDLSYNRDFNKL 588

Query: 586 PSSINQLVNLQTLCLDDCVLPDMSI-VGELKKLEILSLRASDIVALPRVIGQLTNLKMLN 645
           P  I+ LV+LQ L L +  +  M I + ELKKL  L L  +D +     I +L +L++L 
Sbjct: 589 PEQISGLVSLQFLDLSNTSIEHMPIGLKELKKLTFLDLTYTDRLCSISGISRLLSLRLLR 648

Query: 646 LSNCSKLKVIPANL--LSRLTGLCELYLDNSFKLWNVGHANEGNINAGIAELNNLPRLTT 705
           L   SK+    + L  L +L  L EL +  S +L ++              L  L     
Sbjct: 649 LLG-SKVHGDASVLKELQQLQNLQELAITVSAELISLDQ-----------RLAKLISNLC 708

Query: 706 LHVHISDARILPRASLFRNLIGYRILIGDGWDWSGKYETSKTLKLKLESSFQREDAIQAL 765
           +   +     L   +   NL   R+        +  +   K  + + ESS+ R +     
Sbjct: 709 IEGFLQKPFDLSFLASMENLSSLRV-------ENSYFSEIKCRESETESSYLRINPKIPC 768

Query: 766 LENTEDLYLDELRSAKNILFSLDHKGFPKLKRLRVHNNAEIVTVVNSENMQHPHSAFPL- 825
             N   L + +  S K++ + L     P L  L + ++ E+  ++N E   +  S  P  
Sbjct: 769 FTNLSRLEIMKCHSMKDLTWIL---FAPNLVVLLIEDSREVGEIINKEKATNLTSITPFL 828

Query: 826 -LESLSLKNLANLGLICRGTLPQMSFRNLKRVKVESCSRLKFVFPPSMGGGLVLQKLEIS 885
            LE L L NL  L  I    LP   F  L  + V +C +L+   P +      +++ EI 
Sbjct: 829 KLEWLILYNLPKLESIYWSPLP---FPVLLTMDVSNCPKLR-KLPLNATSVSKVEEFEIH 867