MS021964 (gene) Bitter gourd (TR) v1

Overview
NameMS021964
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionsplicing factor 3B subunit 2
Locationscaffold110: 618747 .. 624462 (-)
RNA-Seq ExpressionMS021964
SyntenyMS021964
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGCGGAGGTGATTACTCAGCCGAATGGTGTTGTCACGAATGGCGACTTGGACCTCAATTCTAACCCTAAATCTGGCGCCGCCAAGAAGTCGCGGGAAAGTGAACGGCGTCGTCGTCGGCGAAAGCAGAAGAAGAACCAGAAGGCTTCTAAAGTGAATGAGGCTACTGCTGGTGATGATAGTGATGCTTCTGGTGACGATATGAAGGAGAACGATGATCCACACCAGGTTTTGTTAATTTTTTATGTTACGTTGAAGAAATGTTTGTCAGTTTGTGGTTTAGGGTTTTTTTTTCTCTTAGTATTAGTATTTCGCTATTGTTGGCAAACGATAGTTTATGGAATGAAGATATAGCTAGATAGTTAAAATTTGCAGTTGTTATGTTGAAAGGAGCTTGATATCTAAGACGTCTATTATTGTACAACCATTAGAAGGGGATTAAGTTTGGGTTTAGATGATGCTCTACGTGAGTTATAAATCGAGTGCGTAACAAAGGAATGGTTCCCAAGCCGTAACCCTACTGAATATAGCTAGGTTTTGTTATGACGGTCTTTGGAAAATTATAGAGAATTCATGGATAAACTAATCTTTAAGAAATTGCATCTTATCTTTCTTGGTTCATAGTCCGGCATGACGTGTCATGATACGCCTCGTGATATGTTAGCATCTGTTCCAAAATGCATAAATTTTTTCTGTTAATCCTTTCATTGAGCTAGCCTGCCGGTTGAGAACCCAGCGTTGATCATTTTCTTTGATATATCTACCCAGAATTTGTTTAATAAGGATAATAGACTTAGAATTTTGATTGTTGGACAGCAAGAACATCCATTGGTCAATTCCTTCGTCTTTTTTGTTTCTCTTCATATAGATAGTTGTTTTACAAAATTCATTTATTTTGTTTTTTGATATTATAGGTTGTTGAGAATGTAGAAATTGAATATGTACCAGAGAAGGCTGAATTAGATGATGTCTTGGACGAAGAATTTAGAAAAGTTTTTGAAAAATTCAATTTCAGTGAAGTAGCTGGTGCTGAGGTAAGTATTTACTTCCAGACTAATTTTGATTATCAATTCTGTTTATTCTCTTGTTGTGCAATCTTCTTACCCTTTTTTTTTTGTTGCTATATAGGAGAATGAAAACAAAGACGAATCTGCCCTAAATGCAGCCTCTAAGAAGTCTGACTCGGATTCTGAAGATGAAGAGCTCGATAACCAGCAAAAAGAAAAGGGCCTGTCAAACAAGAAAAAGAAGGTTAAGGGCCTTGCTATTATCTTACCGTTTATACATAAGGAATAGCATTGGTTATTTCTCATCACAATGATTAGAAAATAATTGGTTTTGTCATCTTTCAGTTGCAACGGCGTATGAAAATTGCAGAGCTGAAGCAGATTTGTTCGAGACCAGATGTTGTTGAGGTAGAAATGCAGCACTTTATACTGTATTGTACATATCTTTTACCTTAAAGTTAATCACAAAAGCCCTCAGACCAAAGCGTACCTTTTTTTTTTTGAGTGGGTGTTGTGATATTCTTCTGGACTTATTTATTTACTGTTCAGATCTGGGATGCAACTGCAGCAGATCCTAAGTTACTTGTGTATCTAAAGTCTTATCGCAATACGGTTCCTGTGCCAAGGCATTGGTGTCAGAAAAGGAAATTTTTGCAGGTTTGTAATTCTGCTAATATTAAATTTACAAGTATAAACAAAAAAAAAATTACAAGTGTATATATATCTATGCACAATTGGTGGTATGTTCAACATGAAGGAATGAGAGAGTTGAGTGAGGGAAGATCATGTTGATAAGGTTTCTCTTATATGTACATATCTGGTTCTTGCAGGGGTGTGGCTGTGACACTGTTGAAGCCGGGACTTGTAGGTGTTTGCTAAGAGCACCCTTGGATTATTTATGTTTTAGTATATTTGGTGGTGTGGGTTGGGATGTGAACATTTTTATGTTGCTTTGATGGGTGGATTTAATTGTGTATTGCATGATTTGTTTTCCCTAAAAATATCGTCATCTATATGCTAATCAATTTGCCTTGAAGATTTAGATGCCTTTCCGTTAATAGTGACCTGAATTCTATGATTCTAGTGATGAAATAATCTCTTAGTTTGGATTGTATTTTATTAGTAGTTGGATTTATTTTGTAGGGGAAGCGTGGTATTGAAAAGCAACCTTTCCAACTTCCTGATTTTATTGCTGCAACAGGAATTGAGAAGATTAGACAGGTAGCTTCCATGTACAACTTTCGATAGTTGTCTTTTCTTATCTTCTATCCTTAAATTAATCATTTTCTGATACGATAATATTACTGTTATAATGTAATAATGTTGTTTAGAAAGCTAGTTCATATTATTCTGGCAAAATCATGTAATAATGTAGGTAGTCTAAATATATTTCAATCCTGATTGTATGCACAATGTGGCATCTGAAGTTGGTTATTCTTTATTATATTTGTTACGCAGGCCTACATAGAAAAAGAGGATAGTAAGAAGTTGAAGCAAAAGCAACGAGAACGTATGCAGCCAAAGATGGGAAAGATGGATATTGATTATCAGGTTTTACTGCCTTTAAATTAGATATGTATTTGACTGGTGATATCTAGTTCTATGTATACTGCTGGTGTCTTTGTTATTGTGTTCATAATGTGAACAGTCCAACTAATTCAATTGGCTACTCTGCACTAGGTTCTCCATGATGCTTTTTTTAAGTACCAGACAAAGCCAAAACTGACAACACTTGGAGATTTGTACTATGAAGGGAAAGAATTCGAGGTATTGGTTTGTTATTATTACTGATCTGCAATGCTCTTTGGTCTTTAGGATCCATTGCACGTGGTTATTTTTTCATGATTTCGGACATTGTGCATTTTAAATTTATAAGTAAGGAGCTTTGTAGAAGTCATTATTTGTACATGGTGACTGCTGCTTGTTCGGATACTGGGTAGCTGATCCATGGGGAATTTTTTCTTTTTTTTTTTCCTCCTTGGCATACAGGTTAAGTTAAGGGAGATGAAACCAGGCATGCTGTCACAAGAACTGAAAGAAGCACTTGGTATGCCAGAGGGTGCTCCTCCCCCATGGCTCATTAACATGCAGGTTAGATAAGCAAACTTGTTGTCAAACTCAATTCTGGCCTAATGTTTTAAGCTGTTTTTATTTGTTATCTTACAACTTATATAATTTTTCTTTTCTTATAGAGATATGGCCCTCCACCATCCTACCCCGATCTAAAGATTCCAGGACTCAATGCCCCCATTCCACCTGGAGCTAGCTTCGGTTACCATCCTGGTGGTTGGGGCAAGCCTCCTGTAGATGAAGTAAGCTTTTGACTTTTGTTTTTTATCTTCAGGTTTAGGTCTGCTGCAAAATCTTACTGTTTTACTTTTGTTTCCATGGATAGTATGGCCGTCCACTGTATGGTGATGTTTTTGGCGTTCAGCAGCAGGATCAAGCTAACTATGAGGTGCCTTATTTATTTTTTTTAAGTAAAGACCTTTTTTTTATAGATTTAGTGAAGGATGGATACATCAAAACTAAAAAATGCTCAACTTAAAGAACTTTCTCGAACTAGAGCTCGTTGGTGGAGAAGGGGTACAATTACAAAAGTAGATGGTGAACACCAATTTGAAGATATAAAATTTGCCTAATCCAATGCTTGTTCCTAATGTCTCTATGTATGTACCTGCGGGTTTCTATGTTTTGACTTCAATGTGGGGTTTTTGTTTACGCCAATGACTCTGAAGACACTGAAATTTTATGACATTCTTTTATAGTCAGTTTCTTTCAAGAAAAAGCTTTTGAGAATAGTGTGCTTCTCTCATCCACCACTAATGAGGTCAGTGTATATGTCTGTTATTTGATTGCTTGATGCGTTGTCTAGGAGGAGCCTGTTGATAAGACCAAGCATTGGGGTGATTTGGAGGAAGAGGAAGAGGAAGAAGAAGAGGTGGAAGAGGAGGAAATTGAAGAAGAGGAAATGGAAGATGGCATTGAATCTGTTGATAGTCTATCGAGGTATGCCATTAATCCATTAGCATTTAATGTCAAGCTCAAAGTTTGAAATCTCTTTCTTAGTTTAACCATCTTAATTGTACTTATCTGAAAAATTAAAATCATCTTAAATAATGATCAATGTCCAGGAAGTACAGATATGACATTTTTTTTTCCAAAACATCAAGGGATATGTTGATTAAGACACTTAAATTAACATGAATTATTTAATATATTTGAATATTTTGGTTTATTATGTACTGAGTTATTCCTGTTTGAAGAGAATAGAGATGAAACTATAATTTGGGGTGGTGTGTGGTGGTTGGTGACATGGTTCGAGGCTTCTCCTTTTCTATTCAATAGATGTCTTAATAATCTTTGGCTAGATATAGACTGAATTAAGCAATTAAAATTGTAAAACTTCTCCAATAACTGTTTAGTTCTATCCCAGCCATGTCCAAGCCCAACTGCATGTTTAAAATGAATAAATAATAGATAGAATGCTTATTTAGCCATTTATAATGTGCATCTGTGTTGAAACATGGCTGCATGATAGTGTGTCCCAGACCTGTTGAATATCGAAACCTGAACTTCAAAGTCCATCTGTGCTTCCCGTTCGATTCTGTTCTCTGAATCGATTCAGTTAATAGCTCCAAAATTTTGCATATGCCTGATTTTGGAATCCTATTGTGGTCCATTATTAACCATTGACTTGTTTTCTACAGCACTCCTACTGGTGTCGAGACGCCTGATGTTATTGACCTTCGGAAACAACAGAGGAAGGAACCTGATAGGCCTCTTTACCAAGTGAGTAACAGTCTTTACCAACTCTCTCACAAAACTGAATTTTGATTTACTCTAATCTAACTGTTTGATTTCTTGAAGGTTCTTGAAGAAAAAGAAGAGAGAGTTGCTCCAGGGACTTTGCTTGGAACTACACATACGTATGCAATTTACTTGCTTATATATATATAAAATATAATATATATATATTTTTTTTGATAACCATCCAACTTTCTTATTATTCTTAAATCTGACATTTGATGTGCTCCCTTCAGTTATGTTATTAGTGGCGGTGCTCAAGATAAGACGGGGGCCAAAAGGGTGAGAAACCTTTTGATGTTTCATGTTAATATGTAAAGGATGATTATTCAACAATATGTAATAATCCGTCTCTTCATGGCTATTCATCTTGCAGGTCGATTTGCTTAGAGGTCAAAAATCTGATAAAGTGGATGTTACTTTGCGACCGGAGGAATTGGAAGCTATGGAAAATGTTCTACCTGCGAAGTATGTGATATCACTTATGACTAGGCTACTTAACCATTTTTCGTTTTTAGTTTACTATTTTTAAAAATTATTCTTGCTTCATTTCTTTATTTTCCTTTCACCTCTCTTAAAAACACATCTTAAAAACACGAAATCCTAGCCAAATTTCAAAAGCAAAAACAAGTTTTTTTTAGCTTTAAAAAACTGGCTTAGATTTGTAGGGTAACAAAACAAAGGAATAAACAGAAGTAGGTATTTATAAGCTTATTTTTTTACAAATAGAAAACCAAAAACAAAATGATTATCAAATGGGCTGTTTGGTTCTGTCTGGTTTTAGCATTGATGTTGTAGTGCAGCCCCTGATTCTTCTCTCTTTTCACAGGTACGAGGAAGCTAGGGAAGAGGAGAAGTTGCGGAGTCAGAGGGAGGACTTCAGTGACATGGTTGCAGAG

mRNA sequence

ATGACTGCGGAGGTGATTACTCAGCCGAATGGTGTTGTCACGAATGGCGACTTGGACCTCAATTCTAACCCTAAATCTGGCGCCGCCAAGAAGTCGCGGGAAAGTGAACGGCGTCGTCGTCGGCGAAAGCAGAAGAAGAACCAGAAGGCTTCTAAAGTGAATGAGGCTACTGCTGGTGATGATAGTGATGCTTCTGGTGACGATATGAAGGAGAACGATGATCCACACCAGGTTGTTGAGAATGTAGAAATTGAATATGTACCAGAGAAGGCTGAATTAGATGATGTCTTGGACGAAGAATTTAGAAAAGTTTTTGAAAAATTCAATTTCAGTGAAGTAGCTGGTGCTGAGGAGAATGAAAACAAAGACGAATCTGCCCTAAATGCAGCCTCTAAGAAGTCTGACTCGGATTCTGAAGATGAAGAGCTCGATAACCAGCAAAAAGAAAAGGGCCTGTCAAACAAGAAAAAGAAGTTGCAACGGCGTATGAAAATTGCAGAGCTGAAGCAGATTTGTTCGAGACCAGATGTTGTTGAGATCTGGGATGCAACTGCAGCAGATCCTAAGTTACTTGTGTATCTAAAGTCTTATCGCAATACGGTTCCTGTGCCAAGGCATTGGTGTCAGAAAAGGAAATTTTTGCAGGGGAAGCGTGGTATTGAAAAGCAACCTTTCCAACTTCCTGATTTTATTGCTGCAACAGGAATTGAGAAGATTAGACAGGCCTACATAGAAAAAGAGGATAGTAAGAAGTTGAAGCAAAAGCAACGAGAACGTATGCAGCCAAAGATGGGAAAGATGGATATTGATTATCAGGTTCTCCATGATGCTTTTTTTAAGTACCAGACAAAGCCAAAACTGACAACACTTGGAGATTTGTACTATGAAGGGAAAGAATTCGAGGTTAAGTTAAGGGAGATGAAACCAGGCATGCTGTCACAAGAACTGAAAGAAGCACTTGGTATGCCAGAGGGTGCTCCTCCCCCATGGCTCATTAACATGCAGAGATATGGCCCTCCACCATCCTACCCCGATCTAAAGATTCCAGGACTCAATGCCCCCATTCCACCTGGAGCTAGCTTCGGTTACCATCCTGGTGGTTGGGGCAAGCCTCCTGTAGATGAATATGGCCGTCCACTGTATGGTGATGTTTTTGGCGTTCAGCAGCAGGATCAAGCTAACTATGAGGAGGAGCCTGTTGATAAGACCAAGCATTGGGGTGATTTGGAGGAAGAGGAAGAGGAAGAAGAAGAGGTGGAAGAGGAGGAAATTGAAGAAGAGGAAATGGAAGATGGCATTGAATCTGTTGATAGTCTATCGAGCACTCCTACTGGTGTCGAGACGCCTGATGTTATTGACCTTCGGAAACAACAGAGGAAGGAACCTGATAGGCCTCTTTACCAAGTTCTTGAAGAAAAAGAAGAGAGAGTTGCTCCAGGGACTTTGCTTGGAACTACACATACTTATGTTATTAGTGGCGGTGCTCAAGATAAGACGGGGGCCAAAAGGGTCGATTTGCTTAGAGGTCAAAAATCTGATAAAGTGGATGTTACTTTGCGACCGGAGGAATTGGAAGCTATGGAAAATGTTCTACCTGCGAAGTACGAGGAAGCTAGGGAAGAGGAGAAGTTGCGGAGTCAGAGGGAGGACTTCAGTGACATGGTTGCAGAG

Coding sequence (CDS)

ATGACTGCGGAGGTGATTACTCAGCCGAATGGTGTTGTCACGAATGGCGACTTGGACCTCAATTCTAACCCTAAATCTGGCGCCGCCAAGAAGTCGCGGGAAAGTGAACGGCGTCGTCGTCGGCGAAAGCAGAAGAAGAACCAGAAGGCTTCTAAAGTGAATGAGGCTACTGCTGGTGATGATAGTGATGCTTCTGGTGACGATATGAAGGAGAACGATGATCCACACCAGGTTGTTGAGAATGTAGAAATTGAATATGTACCAGAGAAGGCTGAATTAGATGATGTCTTGGACGAAGAATTTAGAAAAGTTTTTGAAAAATTCAATTTCAGTGAAGTAGCTGGTGCTGAGGAGAATGAAAACAAAGACGAATCTGCCCTAAATGCAGCCTCTAAGAAGTCTGACTCGGATTCTGAAGATGAAGAGCTCGATAACCAGCAAAAAGAAAAGGGCCTGTCAAACAAGAAAAAGAAGTTGCAACGGCGTATGAAAATTGCAGAGCTGAAGCAGATTTGTTCGAGACCAGATGTTGTTGAGATCTGGGATGCAACTGCAGCAGATCCTAAGTTACTTGTGTATCTAAAGTCTTATCGCAATACGGTTCCTGTGCCAAGGCATTGGTGTCAGAAAAGGAAATTTTTGCAGGGGAAGCGTGGTATTGAAAAGCAACCTTTCCAACTTCCTGATTTTATTGCTGCAACAGGAATTGAGAAGATTAGACAGGCCTACATAGAAAAAGAGGATAGTAAGAAGTTGAAGCAAAAGCAACGAGAACGTATGCAGCCAAAGATGGGAAAGATGGATATTGATTATCAGGTTCTCCATGATGCTTTTTTTAAGTACCAGACAAAGCCAAAACTGACAACACTTGGAGATTTGTACTATGAAGGGAAAGAATTCGAGGTTAAGTTAAGGGAGATGAAACCAGGCATGCTGTCACAAGAACTGAAAGAAGCACTTGGTATGCCAGAGGGTGCTCCTCCCCCATGGCTCATTAACATGCAGAGATATGGCCCTCCACCATCCTACCCCGATCTAAAGATTCCAGGACTCAATGCCCCCATTCCACCTGGAGCTAGCTTCGGTTACCATCCTGGTGGTTGGGGCAAGCCTCCTGTAGATGAATATGGCCGTCCACTGTATGGTGATGTTTTTGGCGTTCAGCAGCAGGATCAAGCTAACTATGAGGAGGAGCCTGTTGATAAGACCAAGCATTGGGGTGATTTGGAGGAAGAGGAAGAGGAAGAAGAAGAGGTGGAAGAGGAGGAAATTGAAGAAGAGGAAATGGAAGATGGCATTGAATCTGTTGATAGTCTATCGAGCACTCCTACTGGTGTCGAGACGCCTGATGTTATTGACCTTCGGAAACAACAGAGGAAGGAACCTGATAGGCCTCTTTACCAAGTTCTTGAAGAAAAAGAAGAGAGAGTTGCTCCAGGGACTTTGCTTGGAACTACACATACTTATGTTATTAGTGGCGGTGCTCAAGATAAGACGGGGGCCAAAAGGGTCGATTTGCTTAGAGGTCAAAAATCTGATAAAGTGGATGTTACTTTGCGACCGGAGGAATTGGAAGCTATGGAAAATGTTCTACCTGCGAAGTACGAGGAAGCTAGGGAAGAGGAGAAGTTGCGGAGTCAGAGGGAGGACTTCAGTGACATGGTTGCAGAG

Protein sequence

MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGDDSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEENENKDESALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEIWDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIRQAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEFEVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGASFGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEEEEVEEEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAPGTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAREEEKLRSQREDFSDMVAE
Homology
BLAST of MS021964 vs. NCBI nr
Match: XP_022149861.1 (splicing factor 3B subunit 2 [Momordica charantia])

HSP 1 Score: 1044.3 bits (2699), Expect = 3.8e-301
Identity = 554/557 (99.46%), Postives = 555/557 (99.64%), Query Frame = 0

Query: 1   MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD 60
           MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD
Sbjct: 1   MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD 60

Query: 61  DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEENE 120
           DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAG EENE
Sbjct: 61  DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGTEENE 120

Query: 121 NKDESALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180
           NKD+SALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI
Sbjct: 121 NKDDSALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180

Query: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240
           WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR
Sbjct: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240

Query: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300
           QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF
Sbjct: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300

Query: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360
           EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIP GAS
Sbjct: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPLGAS 360

Query: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEEEEVE 420
           FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEEEEVE
Sbjct: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEEEEVE 420

Query: 421 EEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAPG 480
           EEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAPG
Sbjct: 421 EEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAPG 480

Query: 481 TLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEARE 540
           TLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEARE
Sbjct: 481 TLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEARE 540

Query: 541 EEKLRSQREDFSDMVAE 558
           EEKLRSQREDFSDMVAE
Sbjct: 541 EEKLRSQREDFSDMVAE 557

BLAST of MS021964 vs. NCBI nr
Match: XP_038890808.1 (splicing factor 3B subunit 2 [Benincasa hispida] >XP_038890809.1 splicing factor 3B subunit 2 [Benincasa hispida] >XP_038890810.1 splicing factor 3B subunit 2 [Benincasa hispida])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.5e-289
Identity = 538/558 (96.42%), Postives = 546/558 (97.85%), Query Frame = 0

Query: 1   MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD 60
           MTAEV +QPNGVV+NGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKV EA AG+
Sbjct: 1   MTAEV-SQPNGVVSNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVKEAAAGE 60

Query: 61  DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEENE 120
           DSDASGDD KENDDP QVVE VEIEYVPEKAELDD LDEEFRKVFEKF+FSEVAGAEENE
Sbjct: 61  DSDASGDDTKENDDPLQVVEKVEIEYVPEKAELDDNLDEEFRKVFEKFSFSEVAGAEENE 120

Query: 121 NKDESALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180
           NKDESA NAASKKSDSDS+DEELD QQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI
Sbjct: 121 NKDESAQNAASKKSDSDSDDEELDIQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180

Query: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240
           WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR
Sbjct: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240

Query: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300
           QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF
Sbjct: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300

Query: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360
           EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS
Sbjct: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360

Query: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEE-EEV 420
           FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQ+QANYEEEPVDKTKHWGDLEEEEEEE EE 
Sbjct: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQEQANYEEEPVDKTKHWGDLEEEEEEEVEEE 420

Query: 421 EEEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480
           +EEE+EEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP
Sbjct: 421 DEEELEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480

Query: 481 GTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540
           GTLLGTTHTYVISGG QDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR
Sbjct: 481 GTLLGTTHTYVISGGTQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540

Query: 541 EEEKLRSQREDFSDMVAE 558
           EEEKLRSQREDFSDMVAE
Sbjct: 541 EEEKLRSQREDFSDMVAE 557

BLAST of MS021964 vs. NCBI nr
Match: XP_008441818.1 (PREDICTED: splicing factor 3B subunit 2 [Cucumis melo])

HSP 1 Score: 998.8 bits (2581), Expect = 1.8e-287
Identity = 534/558 (95.70%), Postives = 541/558 (96.95%), Query Frame = 0

Query: 1   MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD 60
           MT EV +QPNGVV+NGDLDLNSNPKSGA KKSRESERRRRRRKQKKNQKASKV EA  GD
Sbjct: 1   MTVEV-SQPNGVVSNGDLDLNSNPKSGAVKKSRESERRRRRRKQKKNQKASKVKEAAGGD 60

Query: 61  DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEENE 120
           DSDASGDD KENDDP QVVE VEIEYVPEKAELDD LDE+FRKVFEKF FSEVAGAEENE
Sbjct: 61  DSDASGDDTKENDDPLQVVEKVEIEYVPEKAELDDSLDEDFRKVFEKFTFSEVAGAEENE 120

Query: 121 NKDESALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180
           NKDESA NA SKKSDSDS+DEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI
Sbjct: 121 NKDESAQNATSKKSDSDSDDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180

Query: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240
           WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR
Sbjct: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240

Query: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300
           QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF
Sbjct: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300

Query: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360
           EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS
Sbjct: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360

Query: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEE-EEV 420
           FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQ+QANYEEEPVDKTKHWGDLEEEEEEE EE 
Sbjct: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQEQANYEEEPVDKTKHWGDLEEEEEEEVEEE 420

Query: 421 EEEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480
           +EEE+EEEEMEDGIESVDS SSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP
Sbjct: 421 DEEELEEEEMEDGIESVDSQSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480

Query: 481 GTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540
           GTLLGTTHTYVISGG QDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR
Sbjct: 481 GTLLGTTHTYVISGGTQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540

Query: 541 EEEKLRSQREDFSDMVAE 558
           EEEKLRSQREDFSDMVAE
Sbjct: 541 EEEKLRSQREDFSDMVAE 557

BLAST of MS021964 vs. NCBI nr
Match: TYK07713.1 (splicing factor 3B subunit 2 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 998.8 bits (2581), Expect = 1.8e-287
Identity = 534/558 (95.70%), Postives = 541/558 (96.95%), Query Frame = 0

Query: 1   MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD 60
           MT EV +QPNGVV+NGDLDLNSNPKSGA KKSRESERRRRRRKQKKNQKASKV EA  GD
Sbjct: 1   MTVEV-SQPNGVVSNGDLDLNSNPKSGAVKKSRESERRRRRRKQKKNQKASKVKEAAGGD 60

Query: 61  DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEENE 120
           DSDASGDD KENDDP QVVE VEIEYVPEKAELDD LDE+FRKVFEKF FSEVAGAEENE
Sbjct: 61  DSDASGDDTKENDDPLQVVEKVEIEYVPEKAELDDSLDEDFRKVFEKFTFSEVAGAEENE 120

Query: 121 NKDESALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180
           NKDESA NA SKKSDSDS+DEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI
Sbjct: 121 NKDESAQNATSKKSDSDSDDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180

Query: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240
           WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR
Sbjct: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240

Query: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300
           QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF
Sbjct: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300

Query: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360
           EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS
Sbjct: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360

Query: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEE-EEV 420
           FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQ+QANYEEEPVDKTKHWGDLEEEEEEE EE 
Sbjct: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQEQANYEEEPVDKTKHWGDLEEEEEEEVEEE 420

Query: 421 EEEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480
           +EEE+EEEEMEDGIESVDS SSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP
Sbjct: 421 DEEELEEEEMEDGIESVDSQSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480

Query: 481 GTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540
           GTLLGTTHTYVISGG QDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR
Sbjct: 481 GTLLGTTHTYVISGGTQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540

Query: 541 EEEKLRSQREDFSDMVAE 558
           EEEKLRSQREDFSDMVAE
Sbjct: 541 EEEKLRSQREDFSDMVAE 557

BLAST of MS021964 vs. NCBI nr
Match: XP_022937998.1 (splicing factor 3B subunit 2-like [Cucurbita moschata])

HSP 1 Score: 991.1 bits (2561), Expect = 3.8e-285
Identity = 533/560 (95.18%), Postives = 542/560 (96.79%), Query Frame = 0

Query: 1   MTAEVITQPNGVVTN-GDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAG 60
           MTAEVI+QPNGVV N GD DLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKV EA  G
Sbjct: 1   MTAEVISQPNGVVANGGDFDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVKEAAGG 60

Query: 61  DDSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEEN 120
           +DSDASGDD KENDD  QVVE VEIEYVPEKAELDD LDEEFR+VFEKFNFS+VAG EEN
Sbjct: 61  EDSDASGDDTKENDDLLQVVEKVEIEYVPEKAELDDSLDEEFRRVFEKFNFSDVAGVEEN 120

Query: 121 ENKDESALNAASKKSDSDSEDEELDNQQKEK-GLSNKKKKLQRRMKIAELKQICSRPDVV 180
           ENKDESA NAASKKSDSDS+DEELDNQQKEK GLSNKKKKLQRRMKIAELKQIC RPDVV
Sbjct: 121 ENKDESAQNAASKKSDSDSDDEELDNQQKEKGGLSNKKKKLQRRMKIAELKQICLRPDVV 180

Query: 181 EIWDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEK 240
           EIWDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEK
Sbjct: 181 EIWDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEK 240

Query: 241 IRQAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGK 300
           IRQAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGK
Sbjct: 241 IRQAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGK 300

Query: 301 EFEVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPG 360
           EFEVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPG
Sbjct: 301 EFEVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPG 360

Query: 361 ASFGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEE-E 420
           ASFGYHPGGWGKPPVDEYGRPLYGDVFGVQQQ+QANYEEEPVDKTKHWGDLEEEEEEE E
Sbjct: 361 ASFGYHPGGWGKPPVDEYGRPLYGDVFGVQQQEQANYEEEPVDKTKHWGDLEEEEEEEVE 420

Query: 421 EVEEEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERV 480
           E +EEEIEEEEM+DGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEE+V
Sbjct: 421 EEDEEEIEEEEMQDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEEKV 480

Query: 481 APGTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEE 540
           APGTLLGTTHTYVISGG QDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEE
Sbjct: 481 APGTLLGTTHTYVISGGTQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEE 540

Query: 541 AREEEKLRSQREDFSDMVAE 558
           AREEEKLRSQREDFSDMVAE
Sbjct: 541 AREEEKLRSQREDFSDMVAE 560

BLAST of MS021964 vs. ExPASy Swiss-Prot
Match: Q13435 (Splicing factor 3B subunit 2 OS=Homo sapiens OX=9606 GN=SF3B2 PE=1 SV=2)

HSP 1 Score: 431.0 bits (1107), Expect = 2.0e-119
Identity = 278/563 (49.38%), Postives = 364/563 (64.65%), Query Frame = 0

Query: 28  AAKKSRESERRRRRRKQKKNQKASKVNEATAGDDSDASGDDMKENDDPHQVVENVEIEYV 87
           +  K  ++ +RR R+K+KK Q+   V+  ++G D +      + +D P     +VEIEYV
Sbjct: 317 SVSKKEKNRKRRNRKKKKKPQRVRGVSSESSG-DREKDSTRSRGSDSP---AADVEIEYV 376

Query: 88  PEKAELDDVLDEEFRKVFEKFNFSEVAGAE---ENENKDESALNAASKK---------SD 147
            E+ E+ +     F+++FE F  ++    E   E E  D+   +AA KK         SD
Sbjct: 377 TEEPEIYEPNFIFFKRIFEAFKLTDDVKKEKEKEPEKLDKLENSAAPKKKGFEEEHKDSD 436

Query: 148 SDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEIWDATAADPKLLVYLK 207
            DS D+E + + +   LS KK +   R  +AELKQ+ +RPDVVE+ D TA DPKLLV+LK
Sbjct: 437 DDSSDDEQEKKPEAPKLSKKKLRRMNRFTVAELKQLVARPDVVEMHDVTAQDPKLLVHLK 496

Query: 208 SYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIRQAYIEKEDSKKLKQK 267
           + RN+VPVPRHWC KRK+LQGKRGIEK PF+LPDFI  TGI+++R+A  EKE+ K +K K
Sbjct: 497 ATRNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKTMKSK 556

Query: 268 QRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEFEVKLREMKPGMLSQE 327
            RE+++PKMGK+DIDYQ LHDAFFK+QTKPKLT  GDLYYEGKEFE +L+E KPG LS E
Sbjct: 557 MREKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRLKEKKPGDLSDE 616

Query: 328 LKEALGMPEG-----APPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGASFGYHPGGWGK 387
           L+ +LGMP G      PPPWLI MQRYGPPPSYP+LKIPGLN+PIP   SFGYH GGWGK
Sbjct: 617 LRISLGMPVGPNAHKVPPPWLIAMQRYGPPPSYPNLKIPGLNSPIPESCSFGYHAGGWGK 676

Query: 388 PPVDEYGRPLYGDVFGVQQQD-QANYEEEPVDKTKHWGDLEEEEEEEEEVEEEEIEEEEM 447
           PPVDE G+PLYGDVFG    + Q   EEE +D+T  WG+LE  +EE  E EEEE  +E+ 
Sbjct: 677 PPVDETGKPLYGDVFGTNAAEFQTKTEEEEIDRTP-WGELEPSDEESSEEEEEEESDEDK 736

Query: 448 ED----------GIESVDSLSSTPTGVETPDVIDLRKQQRKE----PDRP-LYQVLEEKE 507
            D          G+ +    SS P G+ETP++I+LRK++ +E     + P L+ VL EK 
Sbjct: 737 PDETGFITPADSGLITPGGFSSVPAGMETPELIELRKKKIEEAMDGSETPQLFTVLPEKR 796

Query: 508 ERVAPGTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAK 558
                G ++G+TH Y +S     K  A     L+G     V+V L PEELE     +  K
Sbjct: 797 TATVGGAMMGSTHIYDMSTVMSRKGPAPE---LQG-----VEVALAPEELELDPMAMTQK 856

BLAST of MS021964 vs. ExPASy Swiss-Prot
Match: Q9UUI3 (Pre-mRNA-splicing factor sap145 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sap145 PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.3e-89
Identity = 247/579 (42.66%), Postives = 342/579 (59.07%), Query Frame = 0

Query: 18  LDLNSNPKSGAAKKSRESERRRRRR-KQKKNQKASKVNEATAGD------DSDASGDDMK 77
           L+ N+  K+   KKSR   RR +++  ++K    +K+ E  + D      ++D   +D K
Sbjct: 34  LERNNKQKN---KKSRNQVRREKKKLLREKTNSGAKLAEKNSDDKDQLTENNDNLYNDKK 93

Query: 78  ENDDPHQV-----VENVEIEYVPEKAELD--DVLDEEFRKVFEKFNFSEVAGAEEN-ENK 137
            N + +       V+ +    + +  ELD  D L E+F+ VF +F   +  G E++ E+ 
Sbjct: 94  SNGNFYDTNKTDSVDGMVYTTIVDSVELDPNDPLIEQFKDVFNRF---KADGQEKDFEDT 153

Query: 138 DESALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEIWD 197
           D+  +  +  +  S+ E++ L  QQ+EK LS KK +  +RM +A+LK +  + DVVE WD
Sbjct: 154 DKGQIMYSDDEILSEGEEDALQKQQEEK-LSKKKLRKLKRMTVAQLKMLSEKADVVEWWD 213

Query: 198 ATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIRQA 257
            ++ DP  L +LK+Y NTVPVPRHW QKR +L G+RGIE+Q F+LP +I ATGI ++R A
Sbjct: 214 VSSLDPLFLTHLKAYPNTVPVPRHWNQKRDYLSGQRGIERQLFELPSYIRATGIVQMRNA 273

Query: 258 YIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEFEV 317
             E E    L+QK RER+QPKMGK+DIDYQ LHDAFF+YQTKP LT  G+ Y+EGKE E 
Sbjct: 274 VHENEADMPLRQKMRERVQPKMGKLDIDYQKLHDAFFRYQTKPVLTGFGECYFEGKELEA 333

Query: 318 KLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGASFG 377
            ++E +PG +S+EL+EALG+  GAPPPWL  MQRYGPPPSYPDLKIPG+N PIP GA +G
Sbjct: 334 DVKEKRPGDISEELREALGIAPGAPPPWLFAMQRYGPPPSYPDLKIPGVNCPIPTGAQWG 393

Query: 378 YHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEEEEVEE- 437
           +HPGGWGKPPVD++ RPLYGDVFG  +         PV  T+HWG+LEE EEEE   EE 
Sbjct: 394 FHPGGWGKPPVDQFNRPLYGDVFGNVKPRIHAGTGSPV-STQHWGELEEFEEEESSEEEE 453

Query: 438 --------EEIEEEEMEDGIESV-------DSLSSTP------TGVETPDVIDLRKQQRK 497
                   EEI E E  +  +S        + L + P      + VE  D ++LRK  + 
Sbjct: 454 SEDVEYPTEEITERETIEEYQSASEPRSQREDLHAEPLTYFNQSNVEV-DNVELRKNTQP 513

Query: 498 EPD---RPLYQVLEEKEERVAPGTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDV 552
             D   R LYQVL EK   ++    +G  H Y I   A+D    KR        ++K DV
Sbjct: 514 SSDAANRDLYQVLPEKSTNIS--GFMGPQHQYDIP-TAEDTLPQKRNAHSMLSSTNKGDV 573

BLAST of MS021964 vs. ExPASy Swiss-Prot
Match: Q02554 (Cold sensitive U2 snRNA suppressor 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CUS1 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.2e-31
Identity = 123/375 (32.80%), Postives = 194/375 (51.73%), Query Frame = 0

Query: 93  LDDVLDEEFRKVFEKFNFSEVAGAEENENKDESALNAA--------SKKSDSDSEDEELD 152
           +D  L++EF+ V ++F   E    +E   KDE   +          ++K  ++ E E+  
Sbjct: 55  VDAKLEKEFKDVLQRFQVQENDTPKE-ITKDEKNNHVVIVEKNPVMNRKHTAEDELEDTP 114

Query: 153 NQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEIWDATAADPKLLVYLKSYRNTVPVP 212
           +   E+ LS +K++   +  +++LK     P ++E +D  A  P LL  +K  +N +PVP
Sbjct: 115 SDGIEEHLSARKRRKTEKPSLSQLKSQVPYPQIIEWYDCDARYPGLLASIKCTKNVIPVP 174

Query: 213 RHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR----QAYIEKEDSKKLKQKQRERM 272
            HW  K+++L G+  + K+PF+LPD I  T IE++R    Q+ ++ +D K LK+  R R+
Sbjct: 175 SHWQSKKEYLSGRSLLGKRPFELPDIIKKTNIEQMRSTLPQSGLDGQDEKSLKEASRARV 234

Query: 273 QPKMGKMDIDYQVLHDAFFKYQTKPK---LTTLGDLYYEGKEF--EVKLREM----KPGM 332
           QPKMG +D+DY+ LHD FFK     K   L   GD+YYE +    E   + M    +PG 
Sbjct: 235 QPKMGALDLDYKKLHDVFFKIGANWKPDHLLCFGDVYYENRNLFEETNWKRMVDHKRPGR 294

Query: 333 LSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIP--PGASFG-YHPGGW 392
           +SQEL+  + +PEG  PPW + M+  G P  YPDLKI GLN  I    G  +G   P   
Sbjct: 295 ISQELRAIMNLPEGQLPPWCMKMKDIGLPTGYPDLKIAGLNWDITNLKGDVYGKIIPNHH 354

Query: 393 GKPPVDEYGRPLYGDVFGVQ--------QQDQANYE-----EEPVDKTKHWGDLEEEEEE 431
            +    + GR  +G +   +        +  QAN E     ++  D+ +H  D  +E+  
Sbjct: 355 SRS--KKQGRNYFGALISFETPEFENSKEDTQANAENGRQDDKIDDEVEHKLDHFQEDIS 414

BLAST of MS021964 vs. ExPASy TrEMBL
Match: A0A6J1D9P8 (splicing factor 3B subunit 2 OS=Momordica charantia OX=3673 GN=LOC111018187 PE=4 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 1.9e-301
Identity = 554/557 (99.46%), Postives = 555/557 (99.64%), Query Frame = 0

Query: 1   MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD 60
           MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD
Sbjct: 1   MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD 60

Query: 61  DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEENE 120
           DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAG EENE
Sbjct: 61  DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGTEENE 120

Query: 121 NKDESALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180
           NKD+SALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI
Sbjct: 121 NKDDSALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180

Query: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240
           WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR
Sbjct: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240

Query: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300
           QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF
Sbjct: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300

Query: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360
           EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIP GAS
Sbjct: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPLGAS 360

Query: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEEEEVE 420
           FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEEEEVE
Sbjct: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEEEEVE 420

Query: 421 EEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAPG 480
           EEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAPG
Sbjct: 421 EEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAPG 480

Query: 481 TLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEARE 540
           TLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEARE
Sbjct: 481 TLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEARE 540

Query: 541 EEKLRSQREDFSDMVAE 558
           EEKLRSQREDFSDMVAE
Sbjct: 541 EEKLRSQREDFSDMVAE 557

BLAST of MS021964 vs. ExPASy TrEMBL
Match: A0A5D3C716 (Splicing factor 3B subunit 2 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold105G001390 PE=4 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 8.9e-288
Identity = 534/558 (95.70%), Postives = 541/558 (96.95%), Query Frame = 0

Query: 1   MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD 60
           MT EV +QPNGVV+NGDLDLNSNPKSGA KKSRESERRRRRRKQKKNQKASKV EA  GD
Sbjct: 1   MTVEV-SQPNGVVSNGDLDLNSNPKSGAVKKSRESERRRRRRKQKKNQKASKVKEAAGGD 60

Query: 61  DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEENE 120
           DSDASGDD KENDDP QVVE VEIEYVPEKAELDD LDE+FRKVFEKF FSEVAGAEENE
Sbjct: 61  DSDASGDDTKENDDPLQVVEKVEIEYVPEKAELDDSLDEDFRKVFEKFTFSEVAGAEENE 120

Query: 121 NKDESALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180
           NKDESA NA SKKSDSDS+DEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI
Sbjct: 121 NKDESAQNATSKKSDSDSDDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180

Query: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240
           WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR
Sbjct: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240

Query: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300
           QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF
Sbjct: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300

Query: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360
           EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS
Sbjct: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360

Query: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEE-EEV 420
           FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQ+QANYEEEPVDKTKHWGDLEEEEEEE EE 
Sbjct: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQEQANYEEEPVDKTKHWGDLEEEEEEEVEEE 420

Query: 421 EEEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480
           +EEE+EEEEMEDGIESVDS SSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP
Sbjct: 421 DEEELEEEEMEDGIESVDSQSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480

Query: 481 GTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540
           GTLLGTTHTYVISGG QDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR
Sbjct: 481 GTLLGTTHTYVISGGTQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540

Query: 541 EEEKLRSQREDFSDMVAE 558
           EEEKLRSQREDFSDMVAE
Sbjct: 541 EEEKLRSQREDFSDMVAE 557

BLAST of MS021964 vs. ExPASy TrEMBL
Match: A0A1S3B4C3 (splicing factor 3B subunit 2 OS=Cucumis melo OX=3656 GN=LOC103485870 PE=4 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 8.9e-288
Identity = 534/558 (95.70%), Postives = 541/558 (96.95%), Query Frame = 0

Query: 1   MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD 60
           MT EV +QPNGVV+NGDLDLNSNPKSGA KKSRESERRRRRRKQKKNQKASKV EA  GD
Sbjct: 1   MTVEV-SQPNGVVSNGDLDLNSNPKSGAVKKSRESERRRRRRKQKKNQKASKVKEAAGGD 60

Query: 61  DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEENE 120
           DSDASGDD KENDDP QVVE VEIEYVPEKAELDD LDE+FRKVFEKF FSEVAGAEENE
Sbjct: 61  DSDASGDDTKENDDPLQVVEKVEIEYVPEKAELDDSLDEDFRKVFEKFTFSEVAGAEENE 120

Query: 121 NKDESALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180
           NKDESA NA SKKSDSDS+DEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI
Sbjct: 121 NKDESAQNATSKKSDSDSDDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180

Query: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240
           WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR
Sbjct: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240

Query: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300
           QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF
Sbjct: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300

Query: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360
           EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS
Sbjct: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360

Query: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEE-EEV 420
           FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQ+QANYEEEPVDKTKHWGDLEEEEEEE EE 
Sbjct: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQEQANYEEEPVDKTKHWGDLEEEEEEEVEEE 420

Query: 421 EEEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480
           +EEE+EEEEMEDGIESVDS SSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP
Sbjct: 421 DEEELEEEEMEDGIESVDSQSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480

Query: 481 GTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540
           GTLLGTTHTYVISGG QDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR
Sbjct: 481 GTLLGTTHTYVISGGTQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540

Query: 541 EEEKLRSQREDFSDMVAE 558
           EEEKLRSQREDFSDMVAE
Sbjct: 541 EEEKLRSQREDFSDMVAE 557

BLAST of MS021964 vs. ExPASy TrEMBL
Match: A0A6J1FHL4 (splicing factor 3B subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC111444220 PE=4 SV=1)

HSP 1 Score: 991.1 bits (2561), Expect = 1.9e-285
Identity = 533/560 (95.18%), Postives = 542/560 (96.79%), Query Frame = 0

Query: 1   MTAEVITQPNGVVTN-GDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAG 60
           MTAEVI+QPNGVV N GD DLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKV EA  G
Sbjct: 1   MTAEVISQPNGVVANGGDFDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVKEAAGG 60

Query: 61  DDSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEEN 120
           +DSDASGDD KENDD  QVVE VEIEYVPEKAELDD LDEEFR+VFEKFNFS+VAG EEN
Sbjct: 61  EDSDASGDDTKENDDLLQVVEKVEIEYVPEKAELDDSLDEEFRRVFEKFNFSDVAGVEEN 120

Query: 121 ENKDESALNAASKKSDSDSEDEELDNQQKEK-GLSNKKKKLQRRMKIAELKQICSRPDVV 180
           ENKDESA NAASKKSDSDS+DEELDNQQKEK GLSNKKKKLQRRMKIAELKQIC RPDVV
Sbjct: 121 ENKDESAQNAASKKSDSDSDDEELDNQQKEKGGLSNKKKKLQRRMKIAELKQICLRPDVV 180

Query: 181 EIWDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEK 240
           EIWDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEK
Sbjct: 181 EIWDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEK 240

Query: 241 IRQAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGK 300
           IRQAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGK
Sbjct: 241 IRQAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGK 300

Query: 301 EFEVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPG 360
           EFEVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPG
Sbjct: 301 EFEVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPG 360

Query: 361 ASFGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEE-E 420
           ASFGYHPGGWGKPPVDEYGRPLYGDVFGVQQQ+QANYEEEPVDKTKHWGDLEEEEEEE E
Sbjct: 361 ASFGYHPGGWGKPPVDEYGRPLYGDVFGVQQQEQANYEEEPVDKTKHWGDLEEEEEEEVE 420

Query: 421 EVEEEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERV 480
           E +EEEIEEEEM+DGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEE+V
Sbjct: 421 EEDEEEIEEEEMQDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEEKV 480

Query: 481 APGTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEE 540
           APGTLLGTTHTYVISGG QDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEE
Sbjct: 481 APGTLLGTTHTYVISGGTQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEE 540

Query: 541 AREEEKLRSQREDFSDMVAE 558
           AREEEKLRSQREDFSDMVAE
Sbjct: 541 AREEEKLRSQREDFSDMVAE 560

BLAST of MS021964 vs. ExPASy TrEMBL
Match: A0A0A0LMT1 (PSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G075450 PE=4 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 4.2e-285
Identity = 528/558 (94.62%), Postives = 541/558 (96.95%), Query Frame = 0

Query: 1   MTAEVITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAGD 60
           MT EV +QPNGVV+NGDLDLNSNPKSGA KKSRE+ERRRRRRKQKKNQKASKV EA  G+
Sbjct: 1   MTVEV-SQPNGVVSNGDLDLNSNPKSGAVKKSRENERRRRRRKQKKNQKASKVKEAAGGE 60

Query: 61  DSDASGDDMKENDDPHQVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEENE 120
           DSDASGDD KENDDP QVVE VEIEY+PEKAELDD LDE+FRKVFEKF+FSEVAGAEENE
Sbjct: 61  DSDASGDDTKENDDPLQVVEKVEIEYIPEKAELDDSLDEDFRKVFEKFSFSEVAGAEENE 120

Query: 121 NKDESALNAASKKSDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180
           +KDESA NA SKKSDSDS+DEE DNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI
Sbjct: 121 DKDESAQNATSKKSDSDSDDEEHDNQQKEKGLSNKKKKLQRRMKIAELKQICSRPDVVEI 180

Query: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240
           WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR
Sbjct: 181 WDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATGIEKIR 240

Query: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300
           QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF
Sbjct: 241 QAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYYEGKEF 300

Query: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360
           EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS
Sbjct: 301 EVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPIPPGAS 360

Query: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEEE-EEV 420
           FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQ+QANYEEEPVDKTKHWGDLEEEEEEE EE 
Sbjct: 361 FGYHPGGWGKPPVDEYGRPLYGDVFGVQQQEQANYEEEPVDKTKHWGDLEEEEEEEVEEE 420

Query: 421 EEEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480
           +EEE+EEEEMEDGIESVDS SSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP
Sbjct: 421 DEEELEEEEMEDGIESVDSQSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEKEERVAP 480

Query: 481 GTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540
           GTLLGT+HTYVISGG QDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR
Sbjct: 481 GTLLGTSHTYVISGGTQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAR 540

Query: 541 EEEKLRSQREDFSDMVAE 558
           EEEKLRSQREDFSDMVAE
Sbjct: 541 EEEKLRSQREDFSDMVAE 557

BLAST of MS021964 vs. TAIR 10
Match: AT4G21660.1 (proline-rich spliceosome-associated (PSP) family protein )

HSP 1 Score: 786.6 bits (2030), Expect = 1.3e-227
Identity = 435/565 (76.99%), Postives = 486/565 (86.02%), Query Frame = 0

Query: 1   MTAE-VITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAG 60
           MTA+  +   + VV+NGD+  N N  S ++KKSRE +RRRRRRKQKKN KAS+  +  A 
Sbjct: 1   MTADSTVALVHSVVSNGDVS-NGN-TSASSKKSREIDRRRRRRKQKKNNKASQA-DVDAS 60

Query: 61  DDSDASGDDMKENDDPH-QVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEE 120
           D S AS  + KEN DP  QV E + IEYVPE+AE +D  ++EF+++FEKFNF E   +EE
Sbjct: 61  DVSAAS--ESKENTDPQPQVCEQIVIEYVPEQAEFEDGFNDEFKEIFEKFNFREPLASEE 120

Query: 121 NENKDESALNAASKK---SDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRP 180
           +  KDES      KK   SDSDS+D+E DNQ KEKG+SNKKKKLQRRMKIAELKQ+ +RP
Sbjct: 121 DGTKDESEEKEDVKKKVNSDSDSDDDEQDNQNKEKGISNKKKKLQRRMKIAELKQVSARP 180

Query: 181 DVVEIWDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQGKRGIEKQPFQLPDFIAATG 240
           DVVE+WDAT+ADPKLLV+LKSYRNTVPVPRHW QKRK+LQGKRGIEKQPF LPDFIAATG
Sbjct: 181 DVVEVWDATSADPKLLVFLKSYRNTVPVPRHWSQKRKYLQGKRGIEKQPFHLPDFIAATG 240

Query: 241 IEKIRQAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLTTLGDLYY 300
           IEKIRQAYIEKED KKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKL+ LGDLY+
Sbjct: 241 IEKIRQAYIEKEDGKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKYQTKPKLSALGDLYF 300

Query: 301 EGKEFEVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPPSYPDLKIPGLNAPI 360
           EGKEFEVKLRE KPG LS +LKEALGMPEGAPPPWLINMQRYGPPPSYP LKIPGLNAPI
Sbjct: 301 EGKEFEVKLRETKPGFLSNDLKEALGMPEGAPPPWLINMQRYGPPPSYPHLKIPGLNAPI 360

Query: 361 PPGASFGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVDKTKHWGDLEEEEEE 420
           P GASFG+H GGWGKPPVDEYGRPLYGDVFGVQQQDQ NYEEEP+DK+KHWGDLEEEEEE
Sbjct: 361 PIGASFGFHAGGWGKPPVDEYGRPLYGDVFGVQQQDQPNYEEEPIDKSKHWGDLEEEEEE 420

Query: 421 EEEVE---EEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEE 480
           EEE E   EEE++EEE+EDG ESVD+LSSTPTG+ETPD I+LRK QRKEPDR LYQVLEE
Sbjct: 421 EEEEEEEQEEEMDEEELEDGTESVDTLSSTPTGIETPDAIELRKDQRKEPDRALYQVLEE 480

Query: 481 KEERVAPGTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLP 540
           K E VAPGTLLGT+HTYVI  G Q+KTGAKRVDLLRGQK+D+VDV+L+PEEL+AMENVLP
Sbjct: 481 KGESVAPGTLLGTSHTYVIKTGTQEKTGAKRVDLLRGQKTDRVDVSLQPEELDAMENVLP 540

Query: 541 AKYEEAREEEKLRSQREDFSDMVAE 558
           AKYEEAREEEKLR++  D SDMV E
Sbjct: 541 AKYEEAREEEKLRNKPVDLSDMVVE 560

BLAST of MS021964 vs. TAIR 10
Match: AT4G21660.2 (proline-rich spliceosome-associated (PSP) family protein )

HSP 1 Score: 776.9 bits (2005), Expect = 1.1e-224
Identity = 435/579 (75.13%), Postives = 486/579 (83.94%), Query Frame = 0

Query: 1   MTAE-VITQPNGVVTNGDLDLNSNPKSGAAKKSRESERRRRRRKQKKNQKASKVNEATAG 60
           MTA+  +   + VV+NGD+  N N  S ++KKSRE +RRRRRRKQKKN KAS+  +  A 
Sbjct: 1   MTADSTVALVHSVVSNGDVS-NGN-TSASSKKSREIDRRRRRRKQKKNNKASQA-DVDAS 60

Query: 61  DDSDASGDDMKENDDPH-QVVENVEIEYVPEKAELDDVLDEEFRKVFEKFNFSEVAGAEE 120
           D S AS  + KEN DP  QV E + IEYVPE+AE +D  ++EF+++FEKFNF E   +EE
Sbjct: 61  DVSAAS--ESKENTDPQPQVCEQIVIEYVPEQAEFEDGFNDEFKEIFEKFNFREPLASEE 120

Query: 121 NENKDESALNAASKK---SDSDSEDEELDNQQKEKGLSNKKKKLQRRMKIAELKQICSRP 180
           +  KDES      KK   SDSDS+D+E DNQ KEKG+SNKKKKLQRRMKIAELKQ+ +RP
Sbjct: 121 DGTKDESEEKEDVKKKVNSDSDSDDDEQDNQNKEKGISNKKKKLQRRMKIAELKQVSARP 180

Query: 181 DVVEIWDATAADPKLLVYLKSYRNTVPVPRHWCQKRKFLQ--------------GKRGIE 240
           DVVE+WDAT+ADPKLLV+LKSYRNTVPVPRHW QKRK+LQ              GKRGIE
Sbjct: 181 DVVEVWDATSADPKLLVFLKSYRNTVPVPRHWSQKRKYLQGNYIKRDRNVCDNHGKRGIE 240

Query: 241 KQPFQLPDFIAATGIEKIRQAYIEKEDSKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKY 300
           KQPF LPDFIAATGIEKIRQAYIEKED KKLKQKQRERMQPKMGKMDIDYQVLHDAFFKY
Sbjct: 241 KQPFHLPDFIAATGIEKIRQAYIEKEDGKKLKQKQRERMQPKMGKMDIDYQVLHDAFFKY 300

Query: 301 QTKPKLTTLGDLYYEGKEFEVKLREMKPGMLSQELKEALGMPEGAPPPWLINMQRYGPPP 360
           QTKPKL+ LGDLY+EGKEFEVKLRE KPG LS +LKEALGMPEGAPPPWLINMQRYGPPP
Sbjct: 301 QTKPKLSALGDLYFEGKEFEVKLRETKPGFLSNDLKEALGMPEGAPPPWLINMQRYGPPP 360

Query: 361 SYPDLKIPGLNAPIPPGASFGYHPGGWGKPPVDEYGRPLYGDVFGVQQQDQANYEEEPVD 420
           SYP LKIPGLNAPIP GASFG+H GGWGKPPVDEYGRPLYGDVFGVQQQDQ NYEEEP+D
Sbjct: 361 SYPHLKIPGLNAPIPIGASFGFHAGGWGKPPVDEYGRPLYGDVFGVQQQDQPNYEEEPID 420

Query: 421 KTKHWGDLEEEEEEEEEVE---EEEIEEEEMEDGIESVDSLSSTPTGVETPDVIDLRKQQ 480
           K+KHWGDLEEEEEEEEE E   EEE++EEE+EDG ESVD+LSSTPTG+ETPD I+LRK Q
Sbjct: 421 KSKHWGDLEEEEEEEEEEEEEQEEEMDEEELEDGTESVDTLSSTPTGIETPDAIELRKDQ 480

Query: 481 RKEPDRPLYQVLEEKEERVAPGTLLGTTHTYVISGGAQDKTGAKRVDLLRGQKSDKVDVT 540
           RKEPDR LYQVLEEK E VAPGTLLGT+HTYVI  G Q+KTGAKRVDLLRGQK+D+VDV+
Sbjct: 481 RKEPDRALYQVLEEKGESVAPGTLLGTSHTYVIKTGTQEKTGAKRVDLLRGQKTDRVDVS 540

Query: 541 LRPEELEAMENVLPAKYEEAREEEKLRSQREDFSDMVAE 558
           L+PEEL+AMENVLPAKYEEAREEEKLR++  D SDMV E
Sbjct: 541 LQPEELDAMENVLPAKYEEAREEEKLRNKPVDLSDMVVE 574

BLAST of MS021964 vs. TAIR 10
Match: AT1G11520.1 (pliceosome associated protein-related )

HSP 1 Score: 153.7 bits (387), Expect = 4.4e-37
Identity = 85/114 (74.56%), Postives = 96/114 (84.21%), Query Frame = 0

Query: 431 DGIESVDSLSSTPTGVETPDVIDLRKQQRKEPDRPLYQVLEEK-EERVAPGTLLGTTHTY 490
           D ++   SLSSTPTG+ETPD I+LRK+QRKEPDR LYQVLEEK E  VAPGTLL TTHTY
Sbjct: 85  DAMDVSKSLSSTPTGIETPDAIELRKEQRKEPDRALYQVLEEKGESVVAPGTLLRTTHTY 144

Query: 491 VISGGAQDKTGAKRVDLLRGQKSDKVDVTLRPEELEAMENVLPAKYEEAREEEK 544
           VI  G QDKTG KRVDLLRGQK+D+VD +L+PEEL+AM NVL  +YEEAREEEK
Sbjct: 145 VIKTGTQDKTGTKRVDLLRGQKTDRVDFSLQPEELDAMGNVL--QYEEAREEEK 196

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149861.13.8e-30199.46splicing factor 3B subunit 2 [Momordica charantia][more]
XP_038890808.17.5e-28996.42splicing factor 3B subunit 2 [Benincasa hispida] >XP_038890809.1 splicing factor... [more]
XP_008441818.11.8e-28795.70PREDICTED: splicing factor 3B subunit 2 [Cucumis melo][more]
TYK07713.11.8e-28795.70splicing factor 3B subunit 2 isoform X2 [Cucumis melo var. makuwa][more]
XP_022937998.13.8e-28595.18splicing factor 3B subunit 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q134352.0e-11949.38Splicing factor 3B subunit 2 OS=Homo sapiens OX=9606 GN=SF3B2 PE=1 SV=2[more]
Q9UUI31.3e-8942.66Pre-mRNA-splicing factor sap145 OS=Schizosaccharomyces pombe (strain 972 / ATCC ... [more]
Q025541.2e-3132.80Cold sensitive U2 snRNA suppressor 1 OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
Match NameE-valueIdentityDescription
A0A6J1D9P81.9e-30199.46splicing factor 3B subunit 2 OS=Momordica charantia OX=3673 GN=LOC111018187 PE=4... [more]
A0A5D3C7168.9e-28895.70Splicing factor 3B subunit 2 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3B4C38.9e-28895.70splicing factor 3B subunit 2 OS=Cucumis melo OX=3656 GN=LOC103485870 PE=4 SV=1[more]
A0A6J1FHL41.9e-28595.18splicing factor 3B subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC111444220 ... [more]
A0A0A0LMT14.2e-28594.62PSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G075450 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT4G21660.11.3e-22776.99proline-rich spliceosome-associated (PSP) family protein [more]
AT4G21660.21.1e-22475.13proline-rich spliceosome-associated (PSP) family protein [more]
AT1G11520.14.4e-3774.56pliceosome associated protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 149..169
NoneNo IPR availableCOILSCoilCoilcoord: 407..432
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 394..468
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 452..468
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..48
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 410..434
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 116..153
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 537..557
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 63..86
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..86
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 395..409
NoneNo IPR availablePANTHERPTHR12785SPLICING FACTOR 3Bcoord: 2..557
NoneNo IPR availablePANTHERPTHR12785:SF13SPLICING FACTOR 3B SUBUNIT 2-LIKEcoord: 2..557
IPR006568PSP, proline-richSMARTSM00581testneucoord: 304..357
e-value: 1.2E-27
score: 107.8
IPR006568PSP, proline-richPFAMPF04046PSPcoord: 308..353
e-value: 3.4E-22
score: 78.0
IPR007180Domain of unknown function DUF382PFAMPF04037DUF382coord: 174..299
e-value: 2.0E-59
score: 199.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS021964.1MS021964.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus