MS021685 (gene) Bitter gourd (TR) v1

BLAST of MS021685 vs. NCBI nr
Match: XP_022137387.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Momordica charantia])

HSP 1 Score: 1337.4 bits (3460), Expect = 0.0e+00
Identity = 677/677 (100.00%), Postives = 677/677 (100.00%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60
           MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG
Sbjct: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60

Query: 61  EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA 120
           EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA
Sbjct: 61  EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA 120

Query: 121 EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ 180
           EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ
Sbjct: 121 EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ 180

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL
Sbjct: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL 240

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA
Sbjct: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA 300

Query: 301 GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG 360
           GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG
Sbjct: 301 GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG 360

Query: 361 HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL 420
           HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL
Sbjct: 361 HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL 420

Query: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 480
           YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM
Sbjct: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 480

Query: 481 CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA 540
           CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA
Sbjct: 481 CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA 540

Query: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR 600
           KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR
Sbjct: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR 600

Query: 601 ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVRV 660
           ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVRV
Sbjct: 601 ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVRV 660

Query: 661 AAEAAPPHPNAHIAAQN 678
           AAEAAPPHPNAHIAAQN
Sbjct: 661 AAEAAPPHPNAHIAAQN 677

BLAST of MS021685 vs. NCBI nr
Match: XP_038894997.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A isoform X1 [Benincasa hispida])

HSP 1 Score: 1229.5 bits (3180), Expect = 0.0e+00
Identity = 624/682 (91.50%), Postives = 645/682 (94.57%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60
           MQL+TRRLQLIFL LCL S+FINARPFLIVISQDDLKD  P DDSSDSANSD+AEWDEFG
Sbjct: 1   MQLQTRRLQLIFLILCLCSIFINARPFLIVISQDDLKDGAPPDDSSDSANSDSAEWDEFG 60

Query: 61  EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA 120
           EPES  S  ELDPGSWRPIFEPDSPAS  DAPDDLYY+AL KMMSA + GDLR MEDAVA
Sbjct: 61  EPESQNSALELDPGSWRPIFEPDSPAS--DAPDDLYYTALGKMMSAVSSGDLRLMEDAVA 120

Query: 121 EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ 180
           ++DQ A E+GDPHAQSVLGLLYGMGIMKETNKAKAFMYH+FAAEGNKQSKMA+AYIYFRQ
Sbjct: 121 DIDQAAAESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYFRQ 180

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENK ALRKSRGEEDEDFQIL
Sbjct: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 240

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEP+SMELLGEIYARGA
Sbjct: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYARGA 300

Query: 301 GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG 360
           GVER+YTKALQWLT ASKQPSF+AYNGMGYLYVKGYGVEKN+TKAKEYFEKAA NEESGG
Sbjct: 301 GVERDYTKALQWLTRASKQPSFTAYNGMGYLYVKGYGVEKNYTKAKEYFEKAAENEESGG 360

Query: 361 HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL 420
           HYNLGVMYLKGIGVKRDVKKAC HFI+AANAGQPKAFYQLAKMFHTGVGLKRNI MASAL
Sbjct: 361 HYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 420

Query: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 480
           YKLVAERGPWSSLSRWALESYLKSDIGKAF LYARMAELGYEVAQSNAAWILDKYGEQSM
Sbjct: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFLLYARMAELGYEVAQSNAAWILDKYGEQSM 480

Query: 481 CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA 540
           C+GESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGT+VDYDRAAEAYMHA
Sbjct: 481 CLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 540

Query: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR 600
           KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAA+LPVKLAL SLWLRKN 
Sbjct: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRKNH 600

Query: 601 ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVR- 660
           ADSF+VHVIDSLPEVYPKIDAWVE+VLLEEGNATILTLF CLLTVLYLRERQRRHAAVR 
Sbjct: 601 ADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAAVRA 660

Query: 661 ----VAAEAAPPHPNAHIAAQN 678
                AAEA P HPN H+  QN
Sbjct: 661 AAAAAAAEAVPLHPNDHVPPQN 680

BLAST of MS021685 vs. NCBI nr
Match: XP_022924200.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita moschata] >XP_022924201.1 ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita moschata])

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 616/679 (90.72%), Postives = 643/679 (94.70%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60
           MQL+ RR+QLIFL +CL S FINARPFLIVISQDDLKDA   DDSSDSAN D+AEWDEFG
Sbjct: 5   MQLQARRIQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 64

Query: 61  EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA 120
           EPES  + +ELDPG+WRPIFEPDSPAS PDAP+DLYY+AL KMM+A + GDLR MEDAV 
Sbjct: 65  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 124

Query: 121 EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ 180
           ++DQ+A E+GDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMA+AY YFRQ
Sbjct: 125 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 184

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENK ALRKSRGEEDEDFQIL
Sbjct: 185 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 244

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEP+SMELLGEIYARGA
Sbjct: 245 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGA 304

Query: 301 GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG 360
           GVER+YTKALQWLT ASK PSFSAYNG+GYLYVKGYGVEKNFTKAKEYFEKAANN+ESGG
Sbjct: 305 GVERDYTKALQWLTRASKHPSFSAYNGIGYLYVKGYGVEKNFTKAKEYFEKAANNDESGG 364

Query: 361 HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL 420
           HYNLGVMYLKGIGVKRDVKKAC HFIVAANAGQPKAFYQLAKMFHTGVGLKRNI MASAL
Sbjct: 365 HYNLGVMYLKGIGVKRDVKKACTHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 424

Query: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 480
           YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM
Sbjct: 425 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 484

Query: 481 CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA 540
           C+GESGFCTDAE HQRAHSLWWQASEQGNEHAALLIGDAYYYGRGT+VDYDRAAEAYMHA
Sbjct: 485 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 544

Query: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR 600
           KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAA+LPVKLALASLWLRKN 
Sbjct: 545 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 604

Query: 601 ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVR- 660
           ADSF+VHVIDSLPEVYPKID WVE+VLLEEGNATILTLF CLLTVLYLRERQRRHA VR 
Sbjct: 605 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAVVRA 664

Query: 661 -VAAEAAPPHPNAHIAAQN 678
             AAEA P HPN H+AAQN
Sbjct: 665 AAAAEAVPLHPNDHVAAQN 683

BLAST of MS021685 vs. NCBI nr
Match: KAG7019908.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 617/682 (90.47%), Postives = 643/682 (94.28%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60
           MQL+ RRLQLIFL +CL S FINARPFLIVISQDDLKDA   DDSSDSAN D+AEWDEFG
Sbjct: 36  MQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 95

Query: 61  EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA 120
           EPES  + +ELDPG+WRPIFEPDSPAS PDAP+DLYY+AL KMM+A + GDLR MEDAV 
Sbjct: 96  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 155

Query: 121 EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ 180
           ++DQ+A E+GDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMA+AY YFRQ
Sbjct: 156 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 215

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENK ALRKSRGEEDEDFQIL
Sbjct: 216 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 275

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEP+SMELLGEIYARGA
Sbjct: 276 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGA 335

Query: 301 GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG 360
           GVER+YTKALQWLT ASK PSFSAYNG+GYLYVKGYGVEKNFTKAKEYFEKAANN+ESGG
Sbjct: 336 GVERDYTKALQWLTRASKHPSFSAYNGIGYLYVKGYGVEKNFTKAKEYFEKAANNDESGG 395

Query: 361 HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL 420
           HYNLGVMYLKGIGVKRDVKKAC HFIVAANAGQPKAFYQLAKMFHTGVGLKRNI MASAL
Sbjct: 396 HYNLGVMYLKGIGVKRDVKKACTHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 455

Query: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 480
           YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM
Sbjct: 456 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 515

Query: 481 CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA 540
           C+GESGFCTDAE HQRAHSLWWQASEQGNEHAALLIGDAYYYGRGT+VDYDRAAEAYMHA
Sbjct: 516 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 575

Query: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR 600
           KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAA+LPVKLALASLWLRKN 
Sbjct: 576 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 635

Query: 601 ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVR- 660
           ADSF+VHVIDSLPEVYPKID WVE+VLLEEGNATILTLF CLLTVLYLRERQRRHA VR 
Sbjct: 636 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAVVRA 695

Query: 661 ----VAAEAAPPHPNAHIAAQN 678
                AAEA P HPN H+AAQN
Sbjct: 696 AAAAAAAEAVPLHPNDHVAAQN 717

BLAST of MS021685 vs. NCBI nr
Match: KAG6584316.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 617/683 (90.34%), Postives = 643/683 (94.14%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60
           MQL+ RRLQLIFL +CL S FINARPFLIVISQDDLKDA   DDSSDSAN D+AEWDEFG
Sbjct: 5   MQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 64

Query: 61  EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA 120
           EPES  + +ELDPG+WRPIFEPDSPAS PDAP+DLYY+AL KMM+A + GDLR MEDAV 
Sbjct: 65  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 124

Query: 121 EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ 180
           ++DQ+A E+GDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMA+AY YFRQ
Sbjct: 125 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 184

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENK ALRKSRGEEDEDFQIL
Sbjct: 185 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 244

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEP+SMELLGEIYARGA
Sbjct: 245 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGA 304

Query: 301 GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG 360
           GVER+YTKALQWLT ASK PSFSAYNG+GYLYVKGYGVEKNFTKAKEYFEKAANN+ESGG
Sbjct: 305 GVERDYTKALQWLTRASKHPSFSAYNGIGYLYVKGYGVEKNFTKAKEYFEKAANNDESGG 364

Query: 361 HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL 420
           HYNLGVMYLKGIGVKRDVKKAC HFIVAANAGQPKAFYQLAKMFHTGVGLKRNI MASAL
Sbjct: 365 HYNLGVMYLKGIGVKRDVKKACTHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 424

Query: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 480
           YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM
Sbjct: 425 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 484

Query: 481 CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA 540
           C+GESGFCTDAE HQRAHSLWWQASEQGNEHAALLIGDAYYYGRGT+VDYDRAAEAYMHA
Sbjct: 485 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 544

Query: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR 600
           KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAA+LPVKLALASLWLRKN 
Sbjct: 545 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 604

Query: 601 ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVR- 660
           ADSF+VHVIDSLPEVYPKID WVE+VLLEEGNATILTLF CLLTVLYLRERQRRHA VR 
Sbjct: 605 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAVVRA 664

Query: 661 -----VAAEAAPPHPNAHIAAQN 678
                 AAEA P HPN H+AAQN
Sbjct: 665 AAAAAAAAEAVPLHPNDHVAAQN 687

BLAST of MS021685 vs. ExPASy Swiss-Prot
Match: Q9LM25 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A OS=Arabidopsis thaliana OX=3702 GN=HRD3A PE=1 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 2.5e-273
Identity = 475/666 (71.32%), Postives = 564/666 (84.68%), Query Frame = 0

Query: 10  LIFLFLCLYSLF---INARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFGEPESHK 69
           ++ L L ++S     ++ARP ++V+S DDL          D+   +++++DEFGE E  K
Sbjct: 8   IVILSLLVFSFIEFGVHARPVVLVLSNDDLNSG-----GDDNGVGESSDFDEFGESEP-K 67

Query: 70  SDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVAEVDQLA 129
           S+EELDPGSWR IFEPD   S   A    YYS L K++SAA+ G+ R ME+AV E+ + A
Sbjct: 68  SEEELDPGSWRSIFEPDD--STVQAASPQYYSGLKKILSAASEGNFRLMEEAVDEI-EAA 127

Query: 130 TETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMAIAYIYFRQEMYEK 189
           +  GDPHAQS++G +YG+G+M+E +K+K+F++H FAA  GN QSKMA+A+ Y RQ+M++K
Sbjct: 128 SSAGDPHAQSIMGFVYGIGMMREKSKSKSFLHHNFAAAGGNMQSKMALAFTYLRQDMHDK 187

Query: 190 AVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQILEYQAQ 249
           AV+LYAELAE A+NS L+SKDSPV+EP RIH+G EENK ALRKSRGEEDEDFQILEYQAQ
Sbjct: 188 AVQLYAELAETAVNSFLISKDSPVVEPTRIHSGTEENKGALRKSRGEEDEDFQILEYQAQ 247

Query: 250 KGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGAGVERN 309
           KGNA AMY+IGLFYYFGLRGLRRDH KAL WF KAV+KGEPRSMELLGEIYARGAGVERN
Sbjct: 248 KGNANAMYKIGLFYYFGLRGLRRDHTKALHWFLKAVDKGEPRSMELLGEIYARGAGVERN 307

Query: 310 YTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVE-KNFTKAKEYFEKAANNEESGGHYNL 369
           YTKAL+WLTLA+K+  +SA+NG+GYLYVKGYGV+ KN+TKA+EYFEKA +NE+  GHYNL
Sbjct: 308 YTKALEWLTLAAKEGLYSAFNGIGYLYVKGYGVDKKNYTKAREYFEKAVDNEDPSGHYNL 367

Query: 370 GVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASALYKLV 429
           GV+YLKGIGV RDV++A  +F VAANAGQPKAFYQLAKMFHTGVGLK+N+ MA++ YKLV
Sbjct: 368 GVLYLKGIGVNRDVRQATKYFFVAANAGQPKAFYQLAKMFHTGVGLKKNLEMATSFYKLV 427

Query: 430 AERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCMGE 489
           AERGPWSSLSRWALE+YLK D+GKA  LY+RMAE+GYEVAQSNAAWILDKYGE+SMCMG 
Sbjct: 428 AERGPWSSLSRWALEAYLKGDVGKALILYSRMAEMGYEVAQSNAAWILDKYGERSMCMGV 487

Query: 490 SGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHAKSQL 549
           SGFCTD ERH+RAHSLWW+ASEQGNEHAALLIGDAYYYGRGTE D+ RAAEAYMHAKSQ 
Sbjct: 488 SGFCTDKERHERAHSLWWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAYMHAKSQS 547

Query: 550 NAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNRADSF 609
           NAQAMFNLGYMHEHG GLPFDLHLAKRYYD++L+ D AA+LPV LALASLWLR+N AD+ 
Sbjct: 548 NAQAMFNLGYMHEHGQGLPFDLHLAKRYYDESLQSDAAARLPVTLALASLWLRRNYADTV 607

Query: 610 MVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAV---RVA 668
           +V V+DSLPEVYPK++ W+ENV+ EEGNATILTLFVCL+T+LYLRERQRR   V    VA
Sbjct: 608 LVRVVDSLPEVYPKVETWIENVVFEEGNATILTLFVCLITILYLRERQRRQVVVVADPVA 664

BLAST of MS021685 vs. ExPASy Swiss-Prot
Match: Q10NT7 (ERAD-associated E3 ubiquitin-protein ligase component HRD3 OS=Oryza sativa subsp. japonica OX=39947 GN=HRD3 PE=2 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 7.8e-251
Identity = 443/640 (69.22%), Postives = 529/640 (82.66%), Query Frame = 0

Query: 25  RPFLIVISQDD-LKDAPPADDSSDSANSDAAEWDEFGEPESHKSDEELDPGSWRPIFEPD 84
           RPF++V+S+DD LKD   A  S  SA++D+ EWD+F + ES  +D  L P SW P+ +P 
Sbjct: 45  RPFVLVLSRDDFLKDTAGAHPSLPSADADSDEWDDF-DDESPATDPLLSPSSWVPLLDPA 104

Query: 85  SPA-SG--PDAPDD-LYYSALAKMMSAATLGDLRSMEDAVAEVDQLATETGDPHAQSVLG 144
           S + SG  PD+P D L+ + +  M+SAA+ GD  +   A A+++  AT  G P AQS L 
Sbjct: 105 SASPSGDEPDSPSDALFVAGVRAMLSAASAGDDAAFATAAAQIEAAAT-GGHPGAQSALA 164

Query: 145 LLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMAIAYIYFRQEMYEKAVKLYAELAEVAI 204
            L G G+ +  ++++AF+ H FAA+ G+ QSKMA+AY +FRQEMYE+AV LYAELAE A+
Sbjct: 165 FLSGAGMTRPASRSRAFLLHKFAADAGDLQSKMALAYSFFRQEMYEEAVTLYAELAEAAL 224

Query: 205 NSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQILEYQAQKGNAGAMYRIGLF 264
            S L+SK+ PVIEPVR+H+G EENK+ALRKSRGE+DEDFQI EYQAQ+GN  AM+++GL 
Sbjct: 225 TSSLISKEPPVIEPVRLHSGTEENKEALRKSRGEDDEDFQITEYQAQRGNTVAMHKLGLL 284

Query: 265 YYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGAGVERNYTKALQWLTLASK 324
           YY+GLRG+RRD+ KA  WFSKAVEKG+ R+MELLGEIYARGAGVERNYT+A +WLTLA+K
Sbjct: 285 YYYGLRGVRRDYGKAYHWFSKAVEKGDTRAMELLGEIYARGAGVERNYTEAYKWLTLAAK 344

Query: 325 QPSFSAYNGMGYLYVKGYGVE-KNFTKAKEYFEKAANNEESGGHYNLGVMYLKGIGVKRD 384
           Q  +SAYNG+GYLYVKGYGVE KN TKAKE+FE AA ++E GG+YNLGV+YLKGIGVKRD
Sbjct: 345 QQQYSAYNGLGYLYVKGYGVEKKNLTKAKEFFEIAAEHKEHGGYYNLGVLYLKGIGVKRD 404

Query: 385 VKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASALYKLVAERGPWSSLSRWA 444
           V  ACN F+ A NAGQPKA YQ+AK+F  GVGLKRN+ MA+ +YK VAERGPWSSLSRWA
Sbjct: 405 VMTACNFFLRAVNAGQPKAIYQVAKLFQKGVGLKRNLQMAAVMYKSVAERGPWSSLSRWA 464

Query: 445 LESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCMGESGFCTDAERHQRA 504
           LESYLK DIGKA  LY+RMA+LGYEVAQSNAAWILD+YG++S+CMGESGFCTD ERH RA
Sbjct: 465 LESYLKGDIGKALLLYSRMADLGYEVAQSNAAWILDRYGDESICMGESGFCTDMERHLRA 524

Query: 505 HSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHAKSQLNAQAMFNLGYMHE 564
           H+LWWQASEQGNEHAALLIGDAYYYGRG   DY+RAAEAYMHA+SQ NAQAMFNLGYMHE
Sbjct: 525 HALWWQASEQGNEHAALLIGDAYYYGRGVGRDYERAAEAYMHAQSQSNAQAMFNLGYMHE 584

Query: 565 HGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNRADSFMVHVIDSLPEVYP 624
           HG GLP DLHLAKRYYDQA+E+DPAAKLPV LAL SLW+RKN   SF+VH IDSLPEVYP
Sbjct: 585 HGHGLPLDLHLAKRYYDQAVEVDPAAKLPVMLALTSLWIRKNYDGSFLVHFIDSLPEVYP 644

Query: 625 KIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAA 658
            ++ WVE+VL++EGNATI TLF CL+TVLYLRERQRR AA
Sbjct: 645 VVEEWVEDVLMDEGNATIFTLFACLVTVLYLRERQRRQAA 682

BLAST of MS021685 vs. ExPASy Swiss-Prot
Match: Q9C6B6 (ERAD-associated E3 ubiquitin-protein ligase component HRD3B OS=Arabidopsis thaliana OX=3702 GN=HRD3B PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 9.0e-223
Identity = 408/633 (64.45%), Postives = 491/633 (77.57%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLF-LCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEF 60
           M++  + +  I LF L LY   + ARPF++V+S +DL         +D+   +++++DEF
Sbjct: 1   MRVSGQSIIAISLFTLSLYIHRVQARPFVLVLSNEDLNGG-----FNDNGAYESSDFDEF 60

Query: 61  GEPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAV 120
           GE E  KS+EELDPGSWR IFE +       A    YYS L K++SAA+ G+   ME+AV
Sbjct: 61  GESEP-KSEEELDPGSWRRIFETNESTVHASASPQ-YYSGLHKILSAASEGNTTLMEEAV 120

Query: 121 AEVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMAIAYIYF 180
           +E+D  A+ +GDPHAQSV+G +YG+G+M+ET+++K+ ++H+FAA  GN QSKMA+A+ Y 
Sbjct: 121 SEIDSSAS-SGDPHAQSVMGFVYGIGMMRETSRSKSILHHHFAAAGGNMQSKMALAFRYL 180

Query: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQ 240
           RQ MY+KAV+LYAELAE A+NS L+SKDSP+ EPVRIH G EENKDALRKSRGEEDEDFQ
Sbjct: 181 RQNMYDKAVELYAELAETAVNSFLISKDSPMAEPVRIHIGTEENKDALRKSRGEEDEDFQ 240

Query: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYAR 300
           ILEYQA+KGN+ AM++IGLFYYFGLRGLRRDHAKAL WFSKA                  
Sbjct: 241 ILEYQAEKGNSVAMHKIGLFYYFGLRGLRRDHAKALYWFSKA------------------ 300

Query: 301 GAGVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEK-NFTKAKEYFEKAANNEE 360
                                     +NG+GYLYVKGYGV+K N+TKA+EYFE AANNE+
Sbjct: 301 -------------------------EFNGLGYLYVKGYGVDKRNYTKAREYFEMAANNED 360

Query: 361 SGGHYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMA 420
             GHYNLGV+YLKG GVK+DV+ A  +F VAANAGQPKAFYQLAKMFHTGVGL +N+ MA
Sbjct: 361 PSGHYNLGVLYLKGTGVKKDVRHATKYFFVAANAGQPKAFYQLAKMFHTGVGLTKNLEMA 420

Query: 421 SALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGE 480
           +  YKLVAERGPWSSLSRWALE+YLK D+GKAF LY+RM+ELGYEVAQSNAAWI+DKYGE
Sbjct: 421 TTFYKLVAERGPWSSLSRWALEAYLKGDVGKAFILYSRMSELGYEVAQSNAAWIVDKYGE 480

Query: 481 QSMCMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAY 540
           +SMCMG  GFCTD ERH RAHSLWW+ASEQGNEHAALLIGDAYYYGRGTE D+ RAAEAY
Sbjct: 481 RSMCMGVYGFCTDKERHDRAHSLWWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAY 540

Query: 541 MHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLR 600
           M+AKSQ NAQAMFNLGYMHEHG GLPFDLHLAKRYYDQAL+ D AAKLPV LALAS+W+R
Sbjct: 541 MYAKSQSNAQAMFNLGYMHEHGEGLPFDLHLAKRYYDQALQSDTAAKLPVTLALASVWVR 582

Query: 601 KNRADSFMVHVIDSLPEVYPKIDAWVENVLLEE 631
           +N AD+ +V V++SLPEV+ K+  WVEN +LEE
Sbjct: 601 RNYADTALVQVLNSLPEVHQKVVEWVENGMLEE 582

BLAST of MS021685 vs. ExPASy Swiss-Prot
Match: Q9UBV2 (Protein sel-1 homolog 1 OS=Homo sapiens OX=9606 GN=SEL1L PE=1 SV=3)

HSP 1 Score: 283.1 bits (723), Expect = 8.2e-75
Identity = 198/566 (34.98%), Postives = 313/566 (55.30%), Query Frame = 0

Query: 99  ALAKMMSAATLGD-LRSMEDAVAEVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFM 158
           AL ++  A   GD L     A  E+ +  TE G P  Q+ LG LY  G+   +++AKA +
Sbjct: 221 ALERVSYALLFGDYLPQNIQAAREMFEKLTEEGSPKGQTALGFLYASGLGVNSSQAKALV 280

Query: 159 YHYFAA-EGNKQSKMAIAYIYFR----QEMYEKAVKLYAELAEVAINSLLVSKDSPVIEP 218
           Y+ F A  GN  + M + Y Y+      +  E A+  Y  +A    + + ++  S V++ 
Sbjct: 281 YYTFGALGGNLIAHMVLGYRYWAGIGVLQSCESALTHYRLVANHVASDISLTGGS-VVQR 340

Query: 219 VRIHNGAEENKDALRKSRGEEDEDFQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAK 278
           +R+    +E ++    S   E++  Q  ++ A+KG+  A   +G  +  G RG+ ++H +
Sbjct: 341 IRL---PDEVENPGMNSGMLEEDLIQYYQFLAEKGDVQAQVGLGQLHLHGGRGVEQNHQR 400

Query: 279 ALSWFSKAVEKGEPRSMELLGEIYARGAG-VERNYTKALQWLTLASKQPSFSAYNGMGYL 338
           A  +F+ A   G   +M  LG++Y+ G+  V ++   AL +   A+   +    +G+G  
Sbjct: 401 AFDYFNLAANAGNSHAMAFLGKMYSEGSDIVPQSNETALHYFKKAADMGNPVGQSGLGMA 460

Query: 339 YVKGYGVEKNFTKAKEYFEKAANNEESGGHYNLGVMYLKGIGVKRDVKKACNHFIVAANA 398
           Y+ G GV+ N+  A +YF+KAA      G   LG MY  GIGVKRD K+A  +F +A+  
Sbjct: 461 YLYGRGVQVNYDLALKYFQKAAEQGWVDGQLQLGSMYYNGIGVKRDYKQALKYFNLASQG 520

Query: 399 GQPKAFYQLAKMFHTGVGLKRNIAMASALYKLVAERGPWSSLSRWALESYLKSDIGKAFF 458
           G   AFY LA+M  +G G+ R+   A  L+K V ERG WS     A  SY   D   A  
Sbjct: 521 GHILAFYNLAQMHASGTGVMRSCHTAVELFKNVCERGRWSERLMTAYNSYKDGDYNAAVI 580

Query: 459 LYARMAELGYEVAQSNAAWILDKYGEQSMCMGESGFCTDAERHQRAHSLWWQASEQGNEH 518
            Y  +AE GYEVAQSNAA+ILD+   ++  +GE+      E + RA   W +A+ QG   
Sbjct: 581 QYLLLAEQGYEVAQSNAAFILDQ--REASIVGEN------ETYPRALLHWNRAASQGYTV 640

Query: 519 AALLIGDAYYYGRGTEVDYDRAAEAY-MHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAK 578
           A + +GD ++YG GT+VDY+ A   Y + ++ Q +AQAMFNLGYMHE GLG+  D+HLAK
Sbjct: 641 ARIKLGDYHFYGFGTDVDYETAFIHYRLASEQQHSAQAMFNLGYMHEKGLGIKQDIHLAK 700

Query: 579 RYYDQALELDPAAKLPVKLALASLWLRKNRADSFMVHVID-SLPEVYPKIDAWVENVLLE 638
           R+YD A E  P A++PV LAL  L +       F+ ++ + ++ +++ ++D  ++ +L  
Sbjct: 701 RFYDMAAEASPDAQVPVFLALCKLGV-----VYFLQYIRETNIRDMFTQLD--MDQLLGP 760

Query: 639 EGNATILTLFVCLL-TVLYLRERQRR 655
           E +  ++T+   LL TV+  R+RQ +
Sbjct: 761 EWDLYLMTIIALLLGTVIAYRQRQHQ 767

BLAST of MS021685 vs. ExPASy Swiss-Prot
Match: Q9Z2G6 (Protein sel-1 homolog 1 OS=Mus musculus OX=10090 GN=Sel1l PE=1 SV=2)

HSP 1 Score: 280.8 bits (717), Expect = 4.1e-74
Identity = 202/581 (34.77%), Postives = 317/581 (54.56%), Query Frame = 0

Query: 99  ALAKMMSAATLGD-LRSMEDAVAEVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFM 158
           AL ++  A   GD L     A  E+ +  TE G P  Q+ LG LY  G+   +++AKA +
Sbjct: 217 ALERVSYALLFGDYLTQNIQAAKEMFEKLTEEGSPKGQTGLGFLYASGLGVNSSQAKALV 276

Query: 159 YHYFAA-EGNKQSKMAIAYIYFR----QEMYEKAVKLYAELAEVAINSLLVSKDSPVIEP 218
           Y+ F A  GN  + M + Y Y+      +  E A+  Y  +A    + + ++  S V++ 
Sbjct: 277 YYTFGALGGNLIAHMILGYRYWAGIGVLQSCESALTHYRLVANHVASDISLTGGS-VVQR 336

Query: 219 VRIHNGAEENKDALRKSRGEEDEDFQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAK 278
           +R+    +E ++    S   E++  Q  ++ A+KG+  A   +G  +  G RG+ ++H +
Sbjct: 337 IRL---PDEVENPGMNSGMLEEDLIQYYQFLAEKGDVQAQVGLGQLHLHGGRGVEQNHQR 396

Query: 279 ALSWFSKAVEKGEPRSMELLGEIYARGAG-VERNYTKALQWLTLASKQPSFSAYNGMGYL 338
           A  +F+ A   G   +M  LG++Y+ G+  V ++   AL +   A+   +    +G+G  
Sbjct: 397 AFDYFNLAANAGNSHAMAFLGKMYSEGSDIVPQSNETALHYFKKAADMGNPVGQSGLGMA 456

Query: 339 YVKGYGVEKNFTKAKEYFEKAANNEESGGHYNLGVMYLKGIGVKRDVKKACNHFIVAANA 398
           Y+ G GV+ N+  A +YF+KAA      G   LG MY  GIGVKRD K+A  +F +A+  
Sbjct: 457 YLYGRGVQVNYDLALKYFQKAAEQGWVDGQLQLGSMYYNGIGVKRDYKQALKYFNLASQG 516

Query: 399 GQPKAFYQLAKMFHTGVGLKRNIAMASALYKLVAERGPWSSLSRWALESYLKSDIGKAFF 458
           G   AFY LA+M  +G G+ R+   A  L+K V ERG WS     A  SY   D   A  
Sbjct: 517 GHILAFYNLAQMHASGTGVMRSCHTAVELFKNVCERGRWSERLMTAYNSYKDEDYNAAVV 576

Query: 459 LYARMAELGYEVAQSNAAWILDKYGEQSMCMGESGFCTDAERHQRAHSLWWQASEQGNEH 518
            Y  +AE GYEVAQSNAA+ILD+   ++  +GE+      E + RA   W +A+ QG   
Sbjct: 577 QYLLLAEQGYEVAQSNAAFILDQ--REATIVGEN------ETYPRALLHWNRAASQGYTV 636

Query: 519 AALLIGDAYYYGRGTEVDYDRAAEAY-MHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAK 578
           A + +GD ++YG GT+VDY+ A   Y + ++ Q +AQAMFNLGYMHE GLG+  D+HLAK
Sbjct: 637 ARIKLGDYHFYGFGTDVDYETAFIHYRLASEQQHSAQAMFNLGYMHEKGLGIKQDIHLAK 696

Query: 579 RYYDQALELDPAAKLPVKLALASLWLRKNRADSFMVHVID-SLPEVYPKIDAWVENVLLE 638
           R+YD A E  P A++PV LAL  L +       F+ ++ + ++ +++ ++D  ++ +L  
Sbjct: 697 RFYDMAAEASPDAQVPVFLALCKLGV-----VYFLQYIREANIRDLFTQLD--MDQLLGP 756

Query: 639 EGNATILTLFVCLL-TVLYLRERQRRHAAVRVAAEAAPPHP 670
           E +  ++T+   LL TV+  R+RQ +   V       PP P
Sbjct: 757 EWDLYLMTIIALLLGTVIAYRQRQHQDIPV-----PRPPGP 773

BLAST of MS021685 vs. ExPASy TrEMBL
Match: A0A6J1C843 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like OS=Momordica charantia OX=3673 GN=LOC111008853 PE=4 SV=1)

HSP 1 Score: 1337.4 bits (3460), Expect = 0.0e+00
Identity = 677/677 (100.00%), Postives = 677/677 (100.00%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60
           MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG
Sbjct: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60

Query: 61  EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA 120
           EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA
Sbjct: 61  EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA 120

Query: 121 EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ 180
           EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ
Sbjct: 121 EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ 180

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL
Sbjct: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL 240

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA
Sbjct: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA 300

Query: 301 GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG 360
           GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG
Sbjct: 301 GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG 360

Query: 361 HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL 420
           HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL
Sbjct: 361 HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL 420

Query: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 480
           YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM
Sbjct: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 480

Query: 481 CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA 540
           CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA
Sbjct: 481 CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA 540

Query: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR 600
           KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR
Sbjct: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR 600

Query: 601 ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVRV 660
           ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVRV
Sbjct: 601 ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVRV 660

Query: 661 AAEAAPPHPNAHIAAQN 678
           AAEAAPPHPNAHIAAQN
Sbjct: 661 AAEAAPPHPNAHIAAQN 677

BLAST of MS021685 vs. ExPASy TrEMBL
Match: A0A6J1E8X2 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like OS=Cucurbita moschata OX=3662 GN=LOC111431721 PE=4 SV=1)

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 616/679 (90.72%), Postives = 643/679 (94.70%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60
           MQL+ RR+QLIFL +CL S FINARPFLIVISQDDLKDA   DDSSDSAN D+AEWDEFG
Sbjct: 5   MQLQARRIQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 64

Query: 61  EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA 120
           EPES  + +ELDPG+WRPIFEPDSPAS PDAP+DLYY+AL KMM+A + GDLR MEDAV 
Sbjct: 65  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 124

Query: 121 EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ 180
           ++DQ+A E+GDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMA+AY YFRQ
Sbjct: 125 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 184

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENK ALRKSRGEEDEDFQIL
Sbjct: 185 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 244

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEP+SMELLGEIYARGA
Sbjct: 245 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGA 304

Query: 301 GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG 360
           GVER+YTKALQWLT ASK PSFSAYNG+GYLYVKGYGVEKNFTKAKEYFEKAANN+ESGG
Sbjct: 305 GVERDYTKALQWLTRASKHPSFSAYNGIGYLYVKGYGVEKNFTKAKEYFEKAANNDESGG 364

Query: 361 HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL 420
           HYNLGVMYLKGIGVKRDVKKAC HFIVAANAGQPKAFYQLAKMFHTGVGLKRNI MASAL
Sbjct: 365 HYNLGVMYLKGIGVKRDVKKACTHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 424

Query: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 480
           YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM
Sbjct: 425 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 484

Query: 481 CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA 540
           C+GESGFCTDAE HQRAHSLWWQASEQGNEHAALLIGDAYYYGRGT+VDYDRAAEAYMHA
Sbjct: 485 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 544

Query: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR 600
           KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAA+LPVKLALASLWLRKN 
Sbjct: 545 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 604

Query: 601 ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVR- 660
           ADSF+VHVIDSLPEVYPKID WVE+VLLEEGNATILTLF CLLTVLYLRERQRRHA VR 
Sbjct: 605 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAVVRA 664

Query: 661 -VAAEAAPPHPNAHIAAQN 678
             AAEA P HPN H+AAQN
Sbjct: 665 AAAAEAVPLHPNDHVAAQN 683

BLAST of MS021685 vs. ExPASy TrEMBL
Match: A0A0A0LQX2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042850 PE=4 SV=1)

HSP 1 Score: 1221.8 bits (3160), Expect = 0.0e+00
Identity = 620/679 (91.31%), Postives = 642/679 (94.55%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60
           MQL+TRRLQLIFL LCL SLFINARPFLIVISQDDLKD  P DDSSDSANSD+A+WDEFG
Sbjct: 1   MQLQTRRLQLIFLILCLSSLFINARPFLIVISQDDLKDGAPPDDSSDSANSDSADWDEFG 60

Query: 61  EPESHKSDEELDPGSWRPIFEPDSPASG--PDAPDDLYYSALAKMMSAATLGDLRSMEDA 120
           EPES  S  ELDPGSWRPIFEPDS AS    DAP DLYY+AL KMMSA + GDLR MEDA
Sbjct: 61  EPESQNSALELDPGSWRPIFEPDSTASASDSDAPQDLYYTALGKMMSAVSSGDLRLMEDA 120

Query: 121 VAEVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYF 180
           VA++DQ   E+GDPHAQSVLGLLYGMGIMKETNKAKAFMYH+FAAEGNKQSKMA+AYIYF
Sbjct: 121 VADIDQAVAESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYF 180

Query: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQ 240
           RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENK ALRKSRGEEDEDFQ
Sbjct: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 240

Query: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYAR 300
           ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEP+SMELLGEIYAR
Sbjct: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300

Query: 301 GAGVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEES 360
           GAGVER+YTKALQWLT ASKQPSF+AYNGMGYLYVKGYGVEKN+TKAKEYFEKAA N+ES
Sbjct: 301 GAGVERDYTKALQWLTRASKQPSFTAYNGMGYLYVKGYGVEKNYTKAKEYFEKAAENDES 360

Query: 361 GGHYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMAS 420
           GGHYNLGVMYLKGIGVKRDVKKAC HFI+AANAGQPKAFYQLAKMFHTGVGLKRNI MAS
Sbjct: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAS 420

Query: 421 ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480
           ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ
Sbjct: 421 ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480

Query: 481 SMCMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYM 540
           SMC+GESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGT+VDYDRAAEAYM
Sbjct: 481 SMCLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540

Query: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRK 600
           HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAA+LPVKLAL SLWLR 
Sbjct: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRM 600

Query: 601 NRADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAV 660
           N ADSF+VHVIDSLPEVYPKIDAWVE+VLLEEGNATILTLF CLLTVLYLRERQRRHAAV
Sbjct: 601 NHADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAAV 660

Query: 661 RVAAEAAPPHPNAHIAAQN 678
           R AAEA P HPN H+  QN
Sbjct: 661 R-AAEAVPLHPNDHVPPQN 678

BLAST of MS021685 vs. ExPASy TrEMBL
Match: A0A6J1KM40 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like OS=Cucurbita maxima OX=3661 GN=LOC111495816 PE=4 SV=1)

HSP 1 Score: 1221.5 bits (3159), Expect = 0.0e+00
Identity = 615/683 (90.04%), Postives = 643/683 (94.14%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60
           MQL+ RRLQLIFL +CL S FINARPFLIVISQDDLKDA   DDSSDSAN ++AEWDEFG
Sbjct: 13  MQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYESAEWDEFG 72

Query: 61  EPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVA 120
           EPES  + +ELDPG+WRPIFEPDSPAS PDAP+DLYY+AL KMM+A + GDLR MEDAV 
Sbjct: 73  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 132

Query: 121 EVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYFRQ 180
           ++DQ+A E+GDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMA+AY YFRQ
Sbjct: 133 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 192

Query: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQIL 240
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENK ALRKSRGEEDEDFQIL
Sbjct: 193 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 252

Query: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGA 300
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WF+KAV+KGEP+SMELLGEIYARGA
Sbjct: 253 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFAKAVDKGEPKSMELLGEIYARGA 312

Query: 301 GVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEESGG 360
           GVER+YTKALQWLT ASK PSFSAYNG+GYLYVKGYGVEKNFTKAKEYFEKAANN+ESGG
Sbjct: 313 GVERDYTKALQWLTRASKHPSFSAYNGIGYLYVKGYGVEKNFTKAKEYFEKAANNDESGG 372

Query: 361 HYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASAL 420
           HYNLGVMYLKGIGVKRDVKKAC HFIVAANAGQPKAFYQLAKMFHTGVGLKRNI MASAL
Sbjct: 373 HYNLGVMYLKGIGVKRDVKKACTHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIPMASAL 432

Query: 421 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 480
           YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM
Sbjct: 433 YKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 492

Query: 481 CMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHA 540
           C+GESGFCTDAE HQRAHSLWWQASEQGNEHAALLIGDAYYYGRGT+VDYDRAAEAYMHA
Sbjct: 493 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 552

Query: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNR 600
           KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAA+LPVKLALASLWLRKN 
Sbjct: 553 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 612

Query: 601 ADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAVR- 660
           ADSF+VHVIDSLPEVYPKID WVE+VLLEEGNATILTLF CLLTVLYLRERQRRHA VR 
Sbjct: 613 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAVVRA 672

Query: 661 -----VAAEAAPPHPNAHIAAQN 678
                 AAEA P HPN H+AAQN
Sbjct: 673 AAAAAAAAEAVPLHPNDHVAAQN 695

BLAST of MS021685 vs. ExPASy TrEMBL
Match: A0A5D3BIX0 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002400 PE=4 SV=1)

HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 619/683 (90.63%), Postives = 641/683 (93.85%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLFLCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFG 60
           MQL TRR+QLIFL LCL SLFINARPFLIVISQDDLKD  P DDSSDSANSD+A+WDEFG
Sbjct: 1   MQLETRRVQLIFLILCLSSLFINARPFLIVISQDDLKDGAPPDDSSDSANSDSADWDEFG 60

Query: 61  EPESHKSDEELDPGSWRPIFEPDS--PASGPDAPDDLYYSALAKMMSAATLGDLRSMEDA 120
           EPES  S  ELDPGSWRPIFEPDS   AS  DAP DLYY+AL KMMSA + GDLR MEDA
Sbjct: 61  EPESQNSALELDPGSWRPIFEPDSLASASDSDAPQDLYYTALGKMMSAVSSGDLRLMEDA 120

Query: 121 VAEVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMAIAYIYF 180
           VA++DQ   + GDPHAQSVLGLLYGMGIMKETNKAKAFMYH+FAAEGNKQSKMA+AYIYF
Sbjct: 121 VADIDQAVADNGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYF 180

Query: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQ 240
           RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENK ALRKSRGEEDEDFQ
Sbjct: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 240

Query: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYAR 300
           ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEP+SMELLGEIYAR
Sbjct: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300

Query: 301 GAGVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEKNFTKAKEYFEKAANNEES 360
           GAGVER+YTKALQWLT ASKQPSF+AYNGMGYLYVKGYGVEKN+TKAKEYFEKAA N+ES
Sbjct: 301 GAGVERDYTKALQWLTRASKQPSFTAYNGMGYLYVKGYGVEKNYTKAKEYFEKAAENDES 360

Query: 361 GGHYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMAS 420
           GGHYNLGVMYLKGIGVKRDVKKAC HFI+AANAGQPKAFYQLAKMFHTGVGLKRNI MAS
Sbjct: 361 GGHYNLGVMYLKGIGVKRDVKKACTHFIMAANAGQPKAFYQLAKMFHTGVGLKRNIPMAS 420

Query: 421 ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480
           ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ
Sbjct: 421 ALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480

Query: 481 SMCMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYM 540
           SMC+GESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGT+VDYDRAAEAYM
Sbjct: 481 SMCLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540

Query: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRK 600
           HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAA+LPVKLAL SLWLRK
Sbjct: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRK 600

Query: 601 NRADSFMVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAV 660
           N ADSF+VHVIDSLPEVYPKIDAWVE+VLLEEGNATILTLF CLLTVLYLRERQRR AAV
Sbjct: 601 NHADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRERQRRQAAV 660

Query: 661 R---VAAEAAPP-HPNAHIAAQN 678
           R    AAEA PP HPN H+  QN
Sbjct: 661 RAAAAAAEAVPPLHPNDHLPPQN 683

BLAST of MS021685 vs. TAIR 10
Match: AT1G18260.1 (HCP-like superfamily protein )

HSP 1 Score: 942.6 bits (2435), Expect = 1.8e-274
Identity = 475/666 (71.32%), Postives = 564/666 (84.68%), Query Frame = 0

Query: 10  LIFLFLCLYSLF---INARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEFGEPESHK 69
           ++ L L ++S     ++ARP ++V+S DDL          D+   +++++DEFGE E  K
Sbjct: 8   IVILSLLVFSFIEFGVHARPVVLVLSNDDLNSG-----GDDNGVGESSDFDEFGESEP-K 67

Query: 70  SDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAVAEVDQLA 129
           S+EELDPGSWR IFEPD   S   A    YYS L K++SAA+ G+ R ME+AV E+ + A
Sbjct: 68  SEEELDPGSWRSIFEPDD--STVQAASPQYYSGLKKILSAASEGNFRLMEEAVDEI-EAA 127

Query: 130 TETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMAIAYIYFRQEMYEK 189
           +  GDPHAQS++G +YG+G+M+E +K+K+F++H FAA  GN QSKMA+A+ Y RQ+M++K
Sbjct: 128 SSAGDPHAQSIMGFVYGIGMMREKSKSKSFLHHNFAAAGGNMQSKMALAFTYLRQDMHDK 187

Query: 190 AVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQILEYQAQ 249
           AV+LYAELAE A+NS L+SKDSPV+EP RIH+G EENK ALRKSRGEEDEDFQILEYQAQ
Sbjct: 188 AVQLYAELAETAVNSFLISKDSPVVEPTRIHSGTEENKGALRKSRGEEDEDFQILEYQAQ 247

Query: 250 KGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYARGAGVERN 309
           KGNA AMY+IGLFYYFGLRGLRRDH KAL WF KAV+KGEPRSMELLGEIYARGAGVERN
Sbjct: 248 KGNANAMYKIGLFYYFGLRGLRRDHTKALHWFLKAVDKGEPRSMELLGEIYARGAGVERN 307

Query: 310 YTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVE-KNFTKAKEYFEKAANNEESGGHYNL 369
           YTKAL+WLTLA+K+  +SA+NG+GYLYVKGYGV+ KN+TKA+EYFEKA +NE+  GHYNL
Sbjct: 308 YTKALEWLTLAAKEGLYSAFNGIGYLYVKGYGVDKKNYTKAREYFEKAVDNEDPSGHYNL 367

Query: 370 GVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMASALYKLV 429
           GV+YLKGIGV RDV++A  +F VAANAGQPKAFYQLAKMFHTGVGLK+N+ MA++ YKLV
Sbjct: 368 GVLYLKGIGVNRDVRQATKYFFVAANAGQPKAFYQLAKMFHTGVGLKKNLEMATSFYKLV 427

Query: 430 AERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCMGE 489
           AERGPWSSLSRWALE+YLK D+GKA  LY+RMAE+GYEVAQSNAAWILDKYGE+SMCMG 
Sbjct: 428 AERGPWSSLSRWALEAYLKGDVGKALILYSRMAEMGYEVAQSNAAWILDKYGERSMCMGV 487

Query: 490 SGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAYMHAKSQL 549
           SGFCTD ERH+RAHSLWW+ASEQGNEHAALLIGDAYYYGRGTE D+ RAAEAYMHAKSQ 
Sbjct: 488 SGFCTDKERHERAHSLWWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAYMHAKSQS 547

Query: 550 NAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLRKNRADSF 609
           NAQAMFNLGYMHEHG GLPFDLHLAKRYYD++L+ D AA+LPV LALASLWLR+N AD+ 
Sbjct: 548 NAQAMFNLGYMHEHGQGLPFDLHLAKRYYDESLQSDAAARLPVTLALASLWLRRNYADTV 607

Query: 610 MVHVIDSLPEVYPKIDAWVENVLLEEGNATILTLFVCLLTVLYLRERQRRHAAV---RVA 668
           +V V+DSLPEVYPK++ W+ENV+ EEGNATILTLFVCL+T+LYLRERQRR   V    VA
Sbjct: 608 LVRVVDSLPEVYPKVETWIENVVFEEGNATILTLFVCLITILYLRERQRRQVVVVADPVA 664

BLAST of MS021685 vs. TAIR 10
Match: AT1G73570.1 (HCP-like superfamily protein )

HSP 1 Score: 774.6 bits (1999), Expect = 6.4e-224
Identity = 408/633 (64.45%), Postives = 491/633 (77.57%), Query Frame = 0

Query: 1   MQLRTRRLQLIFLF-LCLYSLFINARPFLIVISQDDLKDAPPADDSSDSANSDAAEWDEF 60
           M++  + +  I LF L LY   + ARPF++V+S +DL         +D+   +++++DEF
Sbjct: 1   MRVSGQSIIAISLFTLSLYIHRVQARPFVLVLSNEDLNGG-----FNDNGAYESSDFDEF 60

Query: 61  GEPESHKSDEELDPGSWRPIFEPDSPASGPDAPDDLYYSALAKMMSAATLGDLRSMEDAV 120
           GE E  KS+EELDPGSWR IFE +       A    YYS L K++SAA+ G+   ME+AV
Sbjct: 61  GESEP-KSEEELDPGSWRRIFETNESTVHASASPQ-YYSGLHKILSAASEGNTTLMEEAV 120

Query: 121 AEVDQLATETGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMAIAYIYF 180
           +E+D  A+ +GDPHAQSV+G +YG+G+M+ET+++K+ ++H+FAA  GN QSKMA+A+ Y 
Sbjct: 121 SEIDSSAS-SGDPHAQSVMGFVYGIGMMRETSRSKSILHHHFAAAGGNMQSKMALAFRYL 180

Query: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKDALRKSRGEEDEDFQ 240
           RQ MY+KAV+LYAELAE A+NS L+SKDSP+ EPVRIH G EENKDALRKSRGEEDEDFQ
Sbjct: 181 RQNMYDKAVELYAELAETAVNSFLISKDSPMAEPVRIHIGTEENKDALRKSRGEEDEDFQ 240

Query: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELLGEIYAR 300
           ILEYQA+KGN+ AM++IGLFYYFGLRGLRRDHAKAL WFSKA                  
Sbjct: 241 ILEYQAEKGNSVAMHKIGLFYYFGLRGLRRDHAKALYWFSKA------------------ 300

Query: 301 GAGVERNYTKALQWLTLASKQPSFSAYNGMGYLYVKGYGVEK-NFTKAKEYFEKAANNEE 360
                                     +NG+GYLYVKGYGV+K N+TKA+EYFE AANNE+
Sbjct: 301 -------------------------EFNGLGYLYVKGYGVDKRNYTKAREYFEMAANNED 360

Query: 361 SGGHYNLGVMYLKGIGVKRDVKKACNHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIAMA 420
             GHYNLGV+YLKG GVK+DV+ A  +F VAANAGQPKAFYQLAKMFHTGVGL +N+ MA
Sbjct: 361 PSGHYNLGVLYLKGTGVKKDVRHATKYFFVAANAGQPKAFYQLAKMFHTGVGLTKNLEMA 420

Query: 421 SALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGE 480
           +  YKLVAERGPWSSLSRWALE+YLK D+GKAF LY+RM+ELGYEVAQSNAAWI+DKYGE
Sbjct: 421 TTFYKLVAERGPWSSLSRWALEAYLKGDVGKAFILYSRMSELGYEVAQSNAAWIVDKYGE 480

Query: 481 QSMCMGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTEVDYDRAAEAY 540
           +SMCMG  GFCTD ERH RAHSLWW+ASEQGNEHAALLIGDAYYYGRGTE D+ RAAEAY
Sbjct: 481 RSMCMGVYGFCTDKERHDRAHSLWWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAY 540

Query: 541 MHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAAKLPVKLALASLWLR 600
           M+AKSQ NAQAMFNLGYMHEHG GLPFDLHLAKRYYDQAL+ D AAKLPV LALAS+W+R
Sbjct: 541 MYAKSQSNAQAMFNLGYMHEHGEGLPFDLHLAKRYYDQALQSDTAAKLPVTLALASVWVR 582

Query: 601 KNRADSFMVHVIDSLPEVYPKIDAWVENVLLEE 631
           +N AD+ +V V++SLPEV+ K+  WVEN +LEE
Sbjct: 601 RNYADTALVQVLNSLPEVHQKVVEWVENGMLEE 582