Homology
BLAST of MS021666 vs. NCBI nr
Match:
XP_022954916.1 (nuclear poly(A) polymerase 1-like [Cucurbita moschata])
HSP 1 Score: 1356.7 bits (3510), Expect = 0.0e+00
Identity = 675/744 (90.73%), Postives = 697/744 (93.68%), Query Frame = 0
Query: 1 MGSPALSGRNNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
MGSPAL+GRNNGQRLGITDPISLSGPTE DVIKTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1 MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
Query: 61 GRLDQQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
GRLD QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA
Sbjct: 61 GRLD-QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
Query: 121 TREEDFFGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 180
TREEDFFGELHKMLSEMPEV+ELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD
Sbjct: 121 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 180
Query: 181 ISQDSILQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
+SQDSILQNADEQTVRSLNGCRVTDR+LRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF
Sbjct: 181 VSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
Query: 241 LGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPR 300
LGGINWALLVARICQL+PNALPNMLVSRFFRVFTQWRWPNPVMLC+NEEGSLGL VWDPR
Sbjct: 241 LGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPR 300
Query: 301 RNPKDRFHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
RNPKDR+HLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE
Sbjct: 301 RNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
Query: 361 SYPFFEAYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFS 420
YPFFEAYKNYLQID+TAEN DDLRKWKGWVESRLRQLTLKIERHTYN LQCHPHPGDFS
Sbjct: 361 PYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFS 420
Query: 421 DKSRPFHCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVK 480
DKSRPFH CYFMGLQRKQ G P SGGEQFD+RLTVEEFK SVNMYTLWKPGMEIYVSHVK
Sbjct: 421 DKSRPFHHCYFMGLQRKQ-GVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVK 480
Query: 481 RRNIPNFVFSGGVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRMR 540
+RNIPNFVF GGVRPSRASKVTWDSRRSSELKASDS QVDRPFE ES DGVDDRRKRMR
Sbjct: 481 KRNIPNFVFPGGVRPSRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMR 540
Query: 541 IDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADVSS 600
+DDN NTN RNGEC A HSH EEVHE S++S TSSCS+KD+NS TSANNLEN+ADVSS
Sbjct: 541 LDDNANTNSRNGECLAVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSS 600
Query: 601 QNNGDYGLIGVNPSINIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDDFD 660
QNNGD+G + V+ S NI SAAADTSSCKEAEKLAIQKILSD YDSHQA P E EELDDFD
Sbjct: 601 QNNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFD 660
Query: 661 YKNQAKDFGATKQGSPIILSSAANTSSVVPIPSSNEARQSSCSYSNGGLEELEPAELVAP 720
YKNQAKDFGATKQGSP ++SS ANTSSVVP+ S NEARQSS SYSNGGLEELEPAELVAP
Sbjct: 661 YKNQAKDFGATKQGSP-LMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAP 720
Query: 721 LSTGTAPAPAAGRKPIIRWLCNSL 745
STGTAPAP A RKPIIR SL
Sbjct: 721 SSTGTAPAPVAARKPIIRLSFTSL 741
BLAST of MS021666 vs. NCBI nr
Match:
KAG6572954.1 (Nuclear poly(A) polymerase 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1350.9 bits (3495), Expect = 0.0e+00
Identity = 672/744 (90.32%), Postives = 695/744 (93.41%), Query Frame = 0
Query: 1 MGSPALSGRNNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
MGSPAL+GRNNGQRLGITDPISLSGPTE DVIKTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1 MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
Query: 61 GRLDQQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
GRLD QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA
Sbjct: 61 GRLD-QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
Query: 121 TREEDFFGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 180
TREEDFFGELHKMLSEMPEV+ELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD
Sbjct: 121 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 180
Query: 181 ISQDSILQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
+SQDSILQNADEQTVRSLNGCRVTDR+LRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF
Sbjct: 181 VSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
Query: 241 LGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPR 300
LGGINWALLVARICQL+PNALPNMLVSRFFRVFTQWRWPNPVMLC+NEEGSLGL VWDPR
Sbjct: 241 LGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPR 300
Query: 301 RNPKDRFHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
RNPKDR+HLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE
Sbjct: 301 RNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
Query: 361 SYPFFEAYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFS 420
YPFFEAYKNYLQID+TAEN DDLRKWKGWVESRLRQLTLKIERHTYN LQCHPHPGDFS
Sbjct: 361 PYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFS 420
Query: 421 DKSRPFHCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVK 480
DKS+PFH CYFMGLQRKQ G P SGGEQFD+RLTVEEFK SVNMYTLWKPGMEIYVSHVK
Sbjct: 421 DKSKPFHHCYFMGLQRKQ-GVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVK 480
Query: 481 RRNIPNFVFSGGVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRMR 540
+RNIPNFVF GGVRPSRASKVTWDSRRS ELKASDS QVDRPFE ES DGVDDRRKRMR
Sbjct: 481 KRNIPNFVFPGGVRPSRASKVTWDSRRSLELKASDSAQVDRPFEVAESIDGVDDRRKRMR 540
Query: 541 IDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADVSS 600
+DDN NTN RNGEC A HSH EEVHE S++S TSSCS+KD+NS TSANNLEN+ADVSS
Sbjct: 541 LDDNANTNSRNGECLAVVHSHPEEVHEVSQVSATSSCSIKDLNSTPTSANNLENLADVSS 600
Query: 601 QNNGDYGLIGVNPSINIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDDFD 660
QNNGD+G + V+ S N SAAADTSSCKEAEKLAIQKILSD YDSHQA P E EELDDFD
Sbjct: 601 QNNGDHGSMEVDHSTNSVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFD 660
Query: 661 YKNQAKDFGATKQGSPIILSSAANTSSVVPIPSSNEARQSSCSYSNGGLEELEPAELVAP 720
YKNQAKDFGATKQGSP ++SS ANTSSVVP+ S NEARQSS SYSNGGLEELEPAELVAP
Sbjct: 661 YKNQAKDFGATKQGSP-LMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAP 720
Query: 721 LSTGTAPAPAAGRKPIIRWLCNSL 745
STGTAPAP A RKPIIR SL
Sbjct: 721 SSTGTAPAPVAARKPIIRLSFTSL 741
BLAST of MS021666 vs. NCBI nr
Match:
XP_023541715.1 (nuclear poly(A) polymerase 1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1349.3 bits (3491), Expect = 0.0e+00
Identity = 671/744 (90.19%), Postives = 694/744 (93.28%), Query Frame = 0
Query: 1 MGSPALSGRNNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
MGSPAL+GRNNGQRLGITDPISLSGPTE DVIKTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1 MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
Query: 61 GRLDQQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
GRLD QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA
Sbjct: 61 GRLD-QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
Query: 121 TREEDFFGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 180
TREEDFFGELHKMLSEMPEV+ELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD
Sbjct: 121 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 180
Query: 181 ISQDSILQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
+SQDSILQNADEQTVRSLNGCRVTDR+LRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF
Sbjct: 181 VSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
Query: 241 LGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPR 300
LGGINWALLVARICQL+PNALPNMLVSRFFRVFTQWRWPNPVMLC+NEEGSLGL VWDPR
Sbjct: 241 LGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPR 300
Query: 301 RNPKDRFHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
RNPKDR+HLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE
Sbjct: 301 RNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
Query: 361 SYPFFEAYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFS 420
YPFFEAYKNYLQID+TAEN DDLRKWKGWVESRLRQLTLKIERHTYN LQCHPHPGDFS
Sbjct: 361 PYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFS 420
Query: 421 DKSRPFHCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVK 480
DKS+PFH CYFMGLQRKQ G P SGGEQFD+RLTVEEFK SVNMYTLWKPGMEIYVSHVK
Sbjct: 421 DKSKPFHHCYFMGLQRKQ-GVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVK 480
Query: 481 RRNIPNFVFSGGVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRMR 540
+RNIP FVF GGVRP RASKVTWDSRRSSELKASDS QVDRPFE ES DGVDDRRKRMR
Sbjct: 481 KRNIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMR 540
Query: 541 IDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADVSS 600
+DDN NTN RNGEC HSH EEVHE S++S TSSCS+KD+NS TSANNLEN+ADVSS
Sbjct: 541 LDDNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSS 600
Query: 601 QNNGDYGLIGVNPSINIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDDFD 660
QNNGD+G + V+ S NI SAAADTSSCKEAEKLAIQKILSD YDSHQA P E EELDDFD
Sbjct: 601 QNNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFD 660
Query: 661 YKNQAKDFGATKQGSPIILSSAANTSSVVPIPSSNEARQSSCSYSNGGLEELEPAELVAP 720
YKNQAKDFGATKQGSP ++SS ANTSSVVP+ S NEARQSS SYSNGGLEELEPAELVAP
Sbjct: 661 YKNQAKDFGATKQGSP-LMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAP 720
Query: 721 LSTGTAPAPAAGRKPIIRWLCNSL 745
STGTAPAP A RKPIIR SL
Sbjct: 721 SSTGTAPAPVAARKPIIRLSFTSL 741
BLAST of MS021666 vs. NCBI nr
Match:
XP_022994133.1 (nuclear poly(A) polymerase 1-like [Cucurbita maxima])
HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 670/744 (90.05%), Postives = 693/744 (93.15%), Query Frame = 0
Query: 1 MGSPALSGRNNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
MGSPAL+GRNNGQRLGITDPISLSGPTE DV KTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1 MGSPALNGRNNGQRLGITDPISLSGPTEDDVRKTRELEKYLQDAGLYESQEEAVSREEVL 60
Query: 61 GRLDQQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
GRLD QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA
Sbjct: 61 GRLD-QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
Query: 121 TREEDFFGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 180
TREEDFFGELHKMLSEMPEV+ELHPVPDAHVPVM+FKFAGVSIDLLYAKLSLWVIPEDLD
Sbjct: 121 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMRFKFAGVSIDLLYAKLSLWVIPEDLD 180
Query: 181 ISQDSILQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
+SQDSILQNADEQTVRSLNGCRVTDR+LRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF
Sbjct: 181 VSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
Query: 241 LGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPR 300
LGGINWALLVARICQL+PNALPNMLV RFFRVFTQWRWPNPVMLC+NEEGSLGL VWDPR
Sbjct: 241 LGGINWALLVARICQLFPNALPNMLVYRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPR 300
Query: 301 RNPKDRFHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
RNPKDR+HLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE
Sbjct: 301 RNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
Query: 361 SYPFFEAYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFS 420
YPFFEAYKNYLQID+TAEN DDLRKWKGWVESRLRQLTLKIERHTYN LQCHPHPGDFS
Sbjct: 361 PYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFS 420
Query: 421 DKSRPFHCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVK 480
DKSRPFH CYFMGLQRKQ G P SGGEQFD+RLTVEEFK SVNMYTLWKPGMEIYVSHVK
Sbjct: 421 DKSRPFHHCYFMGLQRKQ-GVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVK 480
Query: 481 RRNIPNFVFSGGVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRMR 540
+RNIPNFVF GGVRPSRASKVTWDSRRSSELKASDS QVDRPFE ES DGVDDRRKRMR
Sbjct: 481 KRNIPNFVFPGGVRPSRASKVTWDSRRSSELKASDSAQVDRPFEVAESMDGVDDRRKRMR 540
Query: 541 IDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADVSS 600
+DDNVNTN RNGEC HSH EEVHE S++S TSSCS+KD+NS TSANNLEN+ADVSS
Sbjct: 541 LDDNVNTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSS 600
Query: 601 QNNGDYGLIGVNPSINIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDDFD 660
QNNGD+G + V+ S NI SAAADTSSCK AEKLAIQKILSD YDSHQA E EELDDFD
Sbjct: 601 QNNGDHGSMEVDHSTNIVSAAADTSSCK-AEKLAIQKILSDSYDSHQALSSELEELDDFD 660
Query: 661 YKNQAKDFGATKQGSPIILSSAANTSSVVPIPSSNEARQSSCSYSNGGLEELEPAELVAP 720
YKNQAKDFGATKQGSP ++SS ANTSSVVP+ S NEARQSS SYSNGGLEELEPAELVAP
Sbjct: 661 YKNQAKDFGATKQGSP-LMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAP 720
Query: 721 LSTGTAPAPAAGRKPIIRWLCNSL 745
STGTAPAP A RKPIIR SL
Sbjct: 721 SSTGTAPAPVAARKPIIRLSFTSL 740
BLAST of MS021666 vs. NCBI nr
Match:
KAG7012136.1 (Nuclear poly(A) polymerase 1 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1327.0 bits (3433), Expect = 0.0e+00
Identity = 671/776 (86.47%), Postives = 694/776 (89.43%), Query Frame = 0
Query: 1 MGSPALSGRNNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
MGSPAL+GRNNGQRLGITDPISLSGPTE DVIKTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1 MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
Query: 61 GRLDQQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLG------------------ 120
GRLD QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLG
Sbjct: 61 GRLD-QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGSQRFSTSIMYKPEDYTMI 120
Query: 121 -----VHGPGADIDTLCVGPRHATREEDFFGELHKMLSEMPEVTELHPVPDAHVPVMKFK 180
VHGPGADIDTLCVGPRHATREEDFFGELHKMLSEMPEV+ELHPVPDAHVPVMKFK
Sbjct: 121 ATVFEVHGPGADIDTLCVGPRHATREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFK 180
Query: 181 FAGVSIDLLYAKLSLWVIPEDLDISQDSILQNADEQTVRSLNGCRVTDRVLRLVPNIQSF 240
FAGVSIDLLYAKLSLWVIPEDLD+SQDSILQNADEQTVRSLNGCRVTDR+LRLVPNIQSF
Sbjct: 181 FAGVSIDLLYAKLSLWVIPEDLDVSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSF 240
Query: 241 RTTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWR 300
RTTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARICQL+PNALPNMLVSRFFRVFTQWR
Sbjct: 241 RTTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWR 300
Query: 301 WPNPVMLCANEEGSLGLQVWDPRRNPKDRFHLMPIITPAYPCMNSSYNVSSSTLRIMTEE 360
WPNPVMLC+NEEGSLGL VWDPRRNPKDR+HLMPIITPAYPCMNSSYNVSSSTLRIMTEE
Sbjct: 301 WPNPVMLCSNEEGSLGLPVWDPRRNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEE 360
Query: 361 FQRGHDICEVMEENKADWDTLFESYPFFEAYKNYLQIDVTAENVDDLRKWKGWVESRLRQ 420
FQRGHDICEVMEENKADWDTLFE YPFFEAYKNYLQID+TAEN DDLRKWKGWVESRLRQ
Sbjct: 361 FQRGHDICEVMEENKADWDTLFEPYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQ 420
Query: 421 LTLK---------IERHTYNMLQCHPHPGDFSDKSRPFHCCYFMGLQRKQGGAPTSGGEQ 480
LTLK IERHTYN LQCHPHPGDFSDKS+PFH CYFMGLQRKQ G P SGGEQ
Sbjct: 421 LTLKIELGSIVMQIERHTYNKLQCHPHPGDFSDKSKPFHHCYFMGLQRKQ-GVPASGGEQ 480
Query: 481 FDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVKRRNIPNFVFSGGVRPSRASKVTWDSRRS 540
FD+RLTVEEFK SVNMYTLWKPGMEIYVSHVK+RNIPNFVF GGVRPSRASKVTWDSRRS
Sbjct: 481 FDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPNFVFPGGVRPSRASKVTWDSRRS 540
Query: 541 SELKASDSTQVDRPFEATESSDGVDDRRKRMRIDDNVNTNFRNGECSAAAHSHTEEVHEA 600
ELKASDS QVDRPFE ES DGVDDRRKRMR+DDN NTN RNGEC A HSH EEVHE
Sbjct: 541 LELKASDSAQVDRPFEVAESIDGVDDRRKRMRLDDNANTNSRNGECLAVVHSHPEEVHEV 600
Query: 601 SRISITSSCSVKDMNSIHTSANNLENMADVSSQNNGDYGLIGVNPSINIESAAADTSSCK 660
S++S TSSCS+KD+NS TSANNLEN+ADVSSQNNGD+G + V+ S N SAAADTSSCK
Sbjct: 601 SQVSATSSCSIKDLNSTPTSANNLENLADVSSQNNGDHGSMEVDHSTNSVSAAADTSSCK 660
Query: 661 EAEKLAIQKILSDPYDSHQAFPCEPEELDDFDYKNQAKDFGATKQGSPIILSSAANTSSV 720
EAEKLAIQKILSD YDSHQA P E EELDDFDYKNQAKDFGATKQGSP ++SS ANTSSV
Sbjct: 661 EAEKLAIQKILSDSYDSHQALPSELEELDDFDYKNQAKDFGATKQGSP-LMSSVANTSSV 720
Query: 721 VPIPSSNEARQSSCSYSNGGLEELEPAELVAPLSTGTAPAPAAGRKPIIRWLCNSL 745
VP+ S NEARQSS SYSNGGLEELEPAELVAP STGTA AP A RKPIIR SL
Sbjct: 721 VPMTSCNEARQSSSSYSNGGLEELEPAELVAPSSTGTAQAPVAARKPIIRLSFTSL 773
BLAST of MS021666 vs. ExPASy Swiss-Prot
Match:
Q9LMT2 (Nuclear poly(A) polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=PAPS1 PE=1 SV=1)
HSP 1 Score: 865.1 bits (2234), Expect = 5.6e-250
Identity = 467/748 (62.43%), Postives = 543/748 (72.59%), Query Frame = 0
Query: 7 SGRNNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQQ 66
S + NGQR G+++PIS+ GPTE+DVIKTRELEK+LQD GLYES+EEAV REEVLG LD Q
Sbjct: 3 SVQQNGQRFGVSEPISMGGPTEFDVIKTRELEKHLQDVGLYESKEEAVRREEVLGILD-Q 62
Query: 67 IVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDF 126
IVK W+K ISRAKGLN+QL+ EANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATRE DF
Sbjct: 63 IVKTWIKTISRAKGLNDQLLHEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREGDF 122
Query: 127 FGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDISQDSI 186
FGEL +MLSEMPEVTELHPVPDAHVP+M FK GVSIDLLYA+L LWVIPEDLD+SQDSI
Sbjct: 123 FGELQRMLSEMPEVTELHPVPDAHVPLMGFKLNGVSIDLLYAQLPLWVIPEDLDLSQDSI 182
Query: 187 LQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINW 246
LQNADEQTVRSLNGCRVTD++LRLVPNIQ+FRTTLRCMRFWAKRRGVYSNVSGFLGGINW
Sbjct: 183 LQNADEQTVRSLNGCRVTDQILRLVPNIQNFRTTLRCMRFWAKRRGVYSNVSGFLGGINW 242
Query: 247 ALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPRRNPKDR 306
ALLVARICQLYPNALPN+LVSRFFRVF QW WPN + LC+ +EGSLGLQVWDPR NPKDR
Sbjct: 243 ALLVARICQLYPNALPNILVSRFFRVFYQWNWPNAIFLCSPDEGSLGLQVWDPRINPKDR 302
Query: 307 FHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFESYPFFE 366
H+MPIITPAYPCMNSSYNVS STLRIM EFQRG++ICE ME NKADWDTLFE + FFE
Sbjct: 303 LHIMPIITPAYPCMNSSYNVSESTLRIMKGEFQRGNEICEAMESNKADWDTLFEPFAFFE 362
Query: 367 AYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFSDKSRPF 426
AYKNYLQID++A NVDDLRKWKGWVESRLRQLTLKIERH + ML CHPHP DF D SRP
Sbjct: 363 AYKNYLQIDISAANVDDLRKWKGWVESRLRQLTLKIERH-FKMLHCHPHPHDFQDTSRPL 422
Query: 427 HCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVKRRNIPN 486
HC YFMGLQRKQ G P + GEQFDIR TVEEFK +VN YTLW PGMEI V H+KRR++PN
Sbjct: 423 HCSYFMGLQRKQ-GVPAAEGEQFDIRRTVEEFKHTVNAYTLWIPGMEISVGHIKRRSLPN 482
Query: 487 FVFSGGVRPSRASKVTWDSRRSSELK--------ASDSTQVDRPFEATESSDGVDDRRKR 546
FVF GGVRPS SK TWDS R SE + A+ +T + E+ S+ D +KR
Sbjct: 483 FVFPGGVRPSHTSKGTWDSNRRSEHRNSSTSSAPAATTTTTEMSSESKAGSNSPVDGKKR 542
Query: 547 MRIDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADV 606
D + + T++ + I+++ + S + S ++ +
Sbjct: 543 KWGD---------------SETLTDQPRNSKHIAVSVPVENCEGGSPNPSVGSICSSPMK 602
Query: 607 SSQNNGDYGLIGVNPSINIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDD 666
NG I +P N+ + + D E L I+KI + +H E EEL++
Sbjct: 603 DYCTNGKSEPISKDPPENVVAFSKD-----PPESLPIEKIATP--QAH-----ETEELEE 662
Query: 667 -FDYKNQAKDFGATKQGSPIILSSAANTSSVVPIPSSNEARQSSCSYSNGGLEELEPAEL 726
FD+ NQ + + K +LS+ T+++ P EA + + +EELE
Sbjct: 663 SFDFGNQVIEQISHKVA---VLSA---TATIPPF----EATSNGSPFPYEAVEELEVLPT 707
Query: 727 VAPLSTGTAPAPAA-GRKPIIRWLCNSL 745
P A P+ RKPII+ SL
Sbjct: 723 RQP---DAAHRPSVQQRKPIIKLSFTSL 707
BLAST of MS021666 vs. ExPASy Swiss-Prot
Match:
Q8VYW1 (Nuclear poly(A) polymerase 4 OS=Arabidopsis thaliana OX=3702 GN=PAPS4 PE=1 SV=1)
HSP 1 Score: 704.5 bits (1817), Expect = 1.3e-201
Identity = 329/495 (66.46%), Postives = 400/495 (80.81%), Query Frame = 0
Query: 10 NNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQQIVK 69
N+ + GIT P+SL+GP+ D+ + ELEKYL D GLYES+++ + REEVLGR+D QIVK
Sbjct: 16 NSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMRREEVLGRID-QIVK 75
Query: 70 IWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGE 129
WVK +++ +G +Q+V++ANA IFTFGSYRLGVHGPGADIDTLCVGP + REEDFF
Sbjct: 76 HWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNREEDFFII 135
Query: 130 LHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDISQDSILQN 189
LH +L+EM EVTELHPVPDAHVPVMKFKF G+ IDLLYA +SL V+P+DLDIS S+L
Sbjct: 136 LHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVPQDLDISSSSVLCE 195
Query: 190 ADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL 249
DE TVRSLNGCRV D++L+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALL
Sbjct: 196 VDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALL 255
Query: 250 VARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPRRNPKDRFHL 309
VAR+CQLYPNA+P+MLVSRFFRV+TQWRWPNPVMLCA EE LG VWD R+N +DR+HL
Sbjct: 256 VARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPVWDRRKNHRDRYHL 315
Query: 310 MPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFESYPFFEAYK 369
MPIITPAYPCMNSSYNVS STLR+MTE+FQ G++I + +E NK W +LFE Y FFEAYK
Sbjct: 316 MPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWSSLFEQYMFFEAYK 375
Query: 370 NYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFSDKSRPF-HC 429
NYLQ+D+ A + +DL WKGWVESR RQLTLKIER T ML CHP P ++ D +R F HC
Sbjct: 376 NYLQVDIVAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTARQFLHC 435
Query: 430 CYFMGLQRKQGGAPTSGG---EQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVKRRNIP 489
+FMGLQR +G GG +QFDIR TV+EF++ VNMY WKPGM+++VSHV+RR +P
Sbjct: 436 AFFMGLQRAEG----VGGQECQQFDIRGTVDEFRQEVNMYMFWKPGMDVFVSHVRRRQLP 495
Query: 490 NFVFSGGVRPSRASK 501
FVF G R R S+
Sbjct: 496 PFVFPNGYRRPRQSR 505
BLAST of MS021666 vs. ExPASy Swiss-Prot
Match:
O82312 (Nuclear poly(A) polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=PAPS2 PE=1 SV=2)
HSP 1 Score: 690.6 bits (1781), Expect = 1.9e-197
Identity = 341/565 (60.35%), Postives = 421/565 (74.51%), Query Frame = 0
Query: 16 GITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQQIVKIWVKAI 75
GIT+P+S++GP+ DV + ELEK+L D GLYES+EE + REEV+ R+D QIVK WVK +
Sbjct: 24 GITEPLSIAGPSAADVKRNLELEKFLVDEGLYESKEETMRREEVVVRID-QIVKHWVKQL 83
Query: 76 SRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGELHKMLS 135
+R +G +Q+V++ANA IFTFGSYRLGVHGP ADIDTLCVGP + REEDFF +L+
Sbjct: 84 TRQRGYTDQMVEDANAVIFTFGSYRLGVHGPMADIDTLCVGPSYVNREEDFFIFFRDILA 143
Query: 136 EMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDISQDSILQNADEQTV 195
EM EVTEL PV DAHVPVMKFKF G+SIDLLYA +SL VIP+DLDIS S+L + DEQTV
Sbjct: 144 EMEEVTELQPVTDAHVPVMKFKFQGISIDLLYASISLLVIPQDLDISNSSVLCDVDEQTV 203
Query: 196 RSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARICQ 255
RSLNGCRV D++L+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALLVAR+CQ
Sbjct: 204 RSLNGCRVADQILKLVPNSEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALLVARLCQ 263
Query: 256 LYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPRRNPKDRFHLMPIITP 315
YPNA+P+MLVSRFFRV+TQWRWPNPVMLCA EE L VWDPR+N +DR+HLMPIITP
Sbjct: 264 FYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDDLSFPVWDPRKNHRDRYHLMPIITP 323
Query: 316 AYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFESYPFFEAYKNYLQID 375
AYPCMNSSYNVS STLR+MTE+FQ G+ IC+ +E NK W +LF+ Y FFEAYKNYLQ+D
Sbjct: 324 AYPCMNSSYNVSQSTLRVMTEQFQFGNTICQEIELNKQHWSSLFQQYMFFEAYKNYLQVD 383
Query: 376 VTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFSDKSRPF-HCCYFMGL 435
V A + +DL WKGWVESR RQLTLKIER T ML CHP P ++ D S+ F HC +FMGL
Sbjct: 384 VLAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTSKQFRHCAFFMGL 443
Query: 436 QRKQGGAPTSGG---EQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVKRRNIPNFVFSG 495
QR G GG +QFDIR TV+EF++ VNMY W+PGM+++VSHV+RR +P+FVF
Sbjct: 444 QRADG----FGGQECQQFDIRGTVDEFRQEVNMYMFWRPGMDVHVSHVRRRQLPSFVFPN 503
Query: 496 GVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRMRIDDNVNTNFRN 555
G + SR S+ +S + + V SD V+ KR D+ +N+
Sbjct: 504 GYKRSRQSR-----HQSQQCREPGDEGVG------SLSDSVERYAKRKNDDEIMNSRPEK 563
Query: 556 GECSAAAHSHTEEVHEASRISITSS 577
E A+ HT + IT+S
Sbjct: 564 REKRASCSLHTLDAASPDSSGITTS 572
BLAST of MS021666 vs. ExPASy Swiss-Prot
Match:
Q54J73 (Poly(A) polymerase OS=Dictyostelium discoideum OX=44689 GN=papA PE=3 SV=1)
HSP 1 Score: 454.5 bits (1168), Expect = 2.3e-126
Identity = 260/606 (42.90%), Postives = 363/606 (59.90%), Query Frame = 0
Query: 15 LGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQQIVKIWVKA 74
LG+T+PIS + P+ D + ELE L L+ES EE+ REE+LG+L+ QIV+ W K
Sbjct: 53 LGVTEPISTAPPSSIDFKLSTELENTLISFNLFESPEESRKREEILGKLN-QIVREWAKQ 112
Query: 75 ISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGELHKML 134
+S KG EQ E AKIFTFGSYRLGVHGPG+DIDTLCVGP+H R DFF +L +L
Sbjct: 113 VSLKKGYPEQTASEVVAKIFTFGSYRLGVHGPGSDIDTLCVGPKHIMR-SDFFDDLSDIL 172
Query: 135 SEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDL-DISQDSILQNADEQ 194
PE+TE V DA VPV+ F+G+ IDL+YAKL+L IPE+L D+ +S L+N DE+
Sbjct: 173 KVHPEITEFTTVKDAFVPVITMVFSGIPIDLIYAKLALTAIPEELNDLIDESFLKNIDEK 232
Query: 195 TVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARI 254
++ SLNGCRVTD++L+LVPNI +FR LRC++ WA RRG+YSN+ GFLGG++WALL ARI
Sbjct: 233 SILSLNGCRVTDQILKLVPNIPNFRMALRCIKLWAIRRGIYSNILGFLGGVSWALLTARI 292
Query: 255 CQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGS-LGLQVWDPRRNPKDRFHLMPI 314
CQLYPN+ P+ ++ RFF+V+ W+WP P++LC +EG LG +VW+P+R D+ HLMPI
Sbjct: 293 CQLYPNSAPSTIIHRFFKVYEIWKWPAPILLCHIQEGGILGPKVWNPKR---DKAHLMPI 352
Query: 315 ITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFESYPFFEAYKNYL 374
ITPAYP MNS+YNVS STL++M EF RG +I +E + W L E FF Y Y+
Sbjct: 353 ITPAYPSMNSTYNVSKSTLQLMKSEFVRGAEITRKIETGECTWKNLLEKCDFFTRYSFYI 412
Query: 375 QIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFSDK----SRPFHC 434
+ID + N +D RKW+GW+ES+LR L +E T M P+P F++ + P
Sbjct: 413 EIDCYSMNEEDSRKWEGWIESKLRFLISNLE-STPKMKFAVPYPKGFTNNLHKANNPDQI 472
Query: 435 C--YFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPG---MEIYVSHVKRRN 494
C +FMGL P + D+ V EF + + +P M+I V ++K++
Sbjct: 473 CTSFFMGLSFNFSNTP-GADKSVDLTKAVTEFTGIIKDWLRTQPNPDTMDIKVQYIKKKQ 532
Query: 495 IPNFVFSGGVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRMRIDD 554
+P FV G P K T +RSS + R +E+SD + K I
Sbjct: 533 LPAFVKDEG--PEEPVKTT--KKRSS----TGEPSATRKKLKSENSDNKLNSPK-SPITT 592
Query: 555 NVNTNFRNGECSAAAHSHTEEVHEASRISIT----SSCSVKDMNSIHTSANNLENMADVS 606
N+N+ + A++ T + + T +S +++S ++ L S
Sbjct: 593 NINSTPTTSTPTTTANTTTNTTTATTTTTTTTVPITSTPTSNISSPTMNSTELTTPTSTS 642
BLAST of MS021666 vs. ExPASy Swiss-Prot
Match:
P51003 (Poly(A) polymerase alpha OS=Homo sapiens OX=9606 GN=PAPOLA PE=1 SV=4)
HSP 1 Score: 438.7 bits (1127), Expect = 1.3e-121
Identity = 237/533 (44.47%), Postives = 333/533 (62.48%), Query Frame = 0
Query: 16 GITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQQIVKIWVKAI 75
GIT PISL+ P E D + T++L + L+ G++E +EE R +LG+L+ +VK W++ I
Sbjct: 21 GITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLN-NLVKEWIREI 80
Query: 76 SRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGELHKMLS 135
S +K L + +++ KIFTFGSYRLGVH GADID LCV PRH R DFF + L
Sbjct: 81 SESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDR-SDFFTSFYDKLK 140
Query: 136 EMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDISQDSILQNADEQTV 195
EV +L V +A VPV+K F G+ ID+L+A+L+L IPEDLD+ DS+L+N D + +
Sbjct: 141 LQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCI 200
Query: 196 RSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARICQ 255
RSLNGCRVTD +L LVPNI +FR TLR ++ WAKR +YSN+ GFLGG++WA+LVAR CQ
Sbjct: 201 RSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQ 260
Query: 256 LYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPRRNPKDRFHLMPIITP 315
LYPNA+ + LV +FF VF++W WPNPV+L EE +L L VWDPR NP DR+HLMPIITP
Sbjct: 261 LYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITP 320
Query: 316 AYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFESYPFFEAYKNYLQID 375
AYP NS+YNVS ST +M EEF++G I + + +KA+W LFE+ FF+ YK+Y+ +
Sbjct: 321 AYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLL 380
Query: 376 VTAENVDDLRKWKGWVESRLRQLTLKIERHTYNML-----QCHPHPGDFSDKSRPFHCCY 435
+A +W G VES++R L +E++ + L Q P P + DK F +
Sbjct: 381 ASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEE-FRTMW 440
Query: 436 FMGLQRKQGGAPTSGGEQFDIRLTVE-------EFKRSVNMYTLWKPGMEIYVSHVKRRN 495
+GL K+ T E + LT + +++++N +++ M+I HVKR+
Sbjct: 441 VIGLVFKK----TENSENLSVDLTYDIQSFTDTVYRQAINS-KMFEVDMKIAAMHVKRKQ 500
Query: 496 ----IPNFVFSGGVRPS-RASKVTW--DSRRSSELKASDSTQVDRPFEATESS 530
+PN V + S K+T DS + + +S V P AT++S
Sbjct: 501 LHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTS 545
BLAST of MS021666 vs. ExPASy TrEMBL
Match:
A0A6J1GS98 (Polynucleotide adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111457031 PE=3 SV=1)
HSP 1 Score: 1356.7 bits (3510), Expect = 0.0e+00
Identity = 675/744 (90.73%), Postives = 697/744 (93.68%), Query Frame = 0
Query: 1 MGSPALSGRNNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
MGSPAL+GRNNGQRLGITDPISLSGPTE DVIKTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1 MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
Query: 61 GRLDQQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
GRLD QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA
Sbjct: 61 GRLD-QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
Query: 121 TREEDFFGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 180
TREEDFFGELHKMLSEMPEV+ELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD
Sbjct: 121 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 180
Query: 181 ISQDSILQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
+SQDSILQNADEQTVRSLNGCRVTDR+LRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF
Sbjct: 181 VSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
Query: 241 LGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPR 300
LGGINWALLVARICQL+PNALPNMLVSRFFRVFTQWRWPNPVMLC+NEEGSLGL VWDPR
Sbjct: 241 LGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPR 300
Query: 301 RNPKDRFHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
RNPKDR+HLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE
Sbjct: 301 RNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
Query: 361 SYPFFEAYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFS 420
YPFFEAYKNYLQID+TAEN DDLRKWKGWVESRLRQLTLKIERHTYN LQCHPHPGDFS
Sbjct: 361 PYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFS 420
Query: 421 DKSRPFHCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVK 480
DKSRPFH CYFMGLQRKQ G P SGGEQFD+RLTVEEFK SVNMYTLWKPGMEIYVSHVK
Sbjct: 421 DKSRPFHHCYFMGLQRKQ-GVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVK 480
Query: 481 RRNIPNFVFSGGVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRMR 540
+RNIPNFVF GGVRPSRASKVTWDSRRSSELKASDS QVDRPFE ES DGVDDRRKRMR
Sbjct: 481 KRNIPNFVFPGGVRPSRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMR 540
Query: 541 IDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADVSS 600
+DDN NTN RNGEC A HSH EEVHE S++S TSSCS+KD+NS TSANNLEN+ADVSS
Sbjct: 541 LDDNANTNSRNGECLAVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSS 600
Query: 601 QNNGDYGLIGVNPSINIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDDFD 660
QNNGD+G + V+ S NI SAAADTSSCKEAEKLAIQKILSD YDSHQA P E EELDDFD
Sbjct: 601 QNNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFD 660
Query: 661 YKNQAKDFGATKQGSPIILSSAANTSSVVPIPSSNEARQSSCSYSNGGLEELEPAELVAP 720
YKNQAKDFGATKQGSP ++SS ANTSSVVP+ S NEARQSS SYSNGGLEELEPAELVAP
Sbjct: 661 YKNQAKDFGATKQGSP-LMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAP 720
Query: 721 LSTGTAPAPAAGRKPIIRWLCNSL 745
STGTAPAP A RKPIIR SL
Sbjct: 721 SSTGTAPAPVAARKPIIRLSFTSL 741
BLAST of MS021666 vs. ExPASy TrEMBL
Match:
A0A6J1K4A6 (Polynucleotide adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111489961 PE=3 SV=1)
HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 670/744 (90.05%), Postives = 693/744 (93.15%), Query Frame = 0
Query: 1 MGSPALSGRNNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60
MGSPAL+GRNNGQRLGITDPISLSGPTE DV KTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1 MGSPALNGRNNGQRLGITDPISLSGPTEDDVRKTRELEKYLQDAGLYESQEEAVSREEVL 60
Query: 61 GRLDQQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
GRLD QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA
Sbjct: 61 GRLD-QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120
Query: 121 TREEDFFGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 180
TREEDFFGELHKMLSEMPEV+ELHPVPDAHVPVM+FKFAGVSIDLLYAKLSLWVIPEDLD
Sbjct: 121 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMRFKFAGVSIDLLYAKLSLWVIPEDLD 180
Query: 181 ISQDSILQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
+SQDSILQNADEQTVRSLNGCRVTDR+LRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF
Sbjct: 181 VSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240
Query: 241 LGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPR 300
LGGINWALLVARICQL+PNALPNMLV RFFRVFTQWRWPNPVMLC+NEEGSLGL VWDPR
Sbjct: 241 LGGINWALLVARICQLFPNALPNMLVYRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPR 300
Query: 301 RNPKDRFHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
RNPKDR+HLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE
Sbjct: 301 RNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360
Query: 361 SYPFFEAYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFS 420
YPFFEAYKNYLQID+TAEN DDLRKWKGWVESRLRQLTLKIERHTYN LQCHPHPGDFS
Sbjct: 361 PYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFS 420
Query: 421 DKSRPFHCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVK 480
DKSRPFH CYFMGLQRKQ G P SGGEQFD+RLTVEEFK SVNMYTLWKPGMEIYVSHVK
Sbjct: 421 DKSRPFHHCYFMGLQRKQ-GVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVK 480
Query: 481 RRNIPNFVFSGGVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRMR 540
+RNIPNFVF GGVRPSRASKVTWDSRRSSELKASDS QVDRPFE ES DGVDDRRKRMR
Sbjct: 481 KRNIPNFVFPGGVRPSRASKVTWDSRRSSELKASDSAQVDRPFEVAESMDGVDDRRKRMR 540
Query: 541 IDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADVSS 600
+DDNVNTN RNGEC HSH EEVHE S++S TSSCS+KD+NS TSANNLEN+ADVSS
Sbjct: 541 LDDNVNTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSS 600
Query: 601 QNNGDYGLIGVNPSINIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDDFD 660
QNNGD+G + V+ S NI SAAADTSSCK AEKLAIQKILSD YDSHQA E EELDDFD
Sbjct: 601 QNNGDHGSMEVDHSTNIVSAAADTSSCK-AEKLAIQKILSDSYDSHQALSSELEELDDFD 660
Query: 661 YKNQAKDFGATKQGSPIILSSAANTSSVVPIPSSNEARQSSCSYSNGGLEELEPAELVAP 720
YKNQAKDFGATKQGSP ++SS ANTSSVVP+ S NEARQSS SYSNGGLEELEPAELVAP
Sbjct: 661 YKNQAKDFGATKQGSP-LMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAP 720
Query: 721 LSTGTAPAPAAGRKPIIRWLCNSL 745
STGTAPAP A RKPIIR SL
Sbjct: 721 SSTGTAPAPVAARKPIIRLSFTSL 740
BLAST of MS021666 vs. ExPASy TrEMBL
Match:
A0A5D3BLG8 (Polynucleotide adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002220 PE=3 SV=1)
HSP 1 Score: 1301.6 bits (3367), Expect = 0.0e+00
Identity = 655/746 (87.80%), Postives = 689/746 (92.36%), Query Frame = 0
Query: 1 MGSPALSGRNNG-QRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEV 60
MGSPAL GRNNG QRLGITDPISLSGPTEYDV+KTRELEKYLQDAGLYESQEEAV+REEV
Sbjct: 1 MGSPALCGRNNGQQRLGITDPISLSGPTEYDVLKTRELEKYLQDAGLYESQEEAVNREEV 60
Query: 61 LGRLDQQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRH 120
LGRLD QIVKIWVKAISR+KGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRH
Sbjct: 61 LGRLD-QIVKIWVKAISRSKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRH 120
Query: 121 ATREEDFFGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDL 180
ATREEDFFGELHKMLSEMPEV+ELHPVPDAHVPVM+FK +GVSIDLLYAKLSLWVIPEDL
Sbjct: 121 ATREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMRFKLSGVSIDLLYAKLSLWVIPEDL 180
Query: 181 DISQDSILQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSG 240
DISQ+SILQN DEQTVRSLNGCRVTDR+LRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSG
Sbjct: 181 DISQESILQNTDEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSG 240
Query: 241 FLGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDP 300
FLGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGL VWDP
Sbjct: 241 FLGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLPVWDP 300
Query: 301 RRNPKDRFHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLF 360
RRNPKDR+HLMPIITPAYPCMNSSYNVS+STLRIMTEEFQRGHDICEVMEENKADWDTLF
Sbjct: 301 RRNPKDRYHLMPIITPAYPCMNSSYNVSASTLRIMTEEFQRGHDICEVMEENKADWDTLF 360
Query: 361 ESYPFFEAYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDF 420
E YPFFEAYKNYLQID+TAEN DD+R WKGWVESRLRQLTLKIERHTYNMLQCHP+PGDF
Sbjct: 361 EPYPFFEAYKNYLQIDITAENDDDIRIWKGWVESRLRQLTLKIERHTYNMLQCHPYPGDF 420
Query: 421 SDKSRPFHCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHV 480
SDKSRPFH CYFMGLQRKQGG P SG EQFDIRLTV+EFKRSVN+YT K GMEIYVSHV
Sbjct: 421 SDKSRPFHHCYFMGLQRKQGG-PASGSEQFDIRLTVDEFKRSVNVYTQRKRGMEIYVSHV 480
Query: 481 KRRNIPNFVFSGGVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRM 540
KRR+IPNFVF GGVRPSRASK+TWD RRSSELKASDSTQVD P E TES DG DDRRK++
Sbjct: 481 KRRSIPNFVFPGGVRPSRASKLTWDIRRSSELKASDSTQVDSPSEVTESLDG-DDRRKKI 540
Query: 541 RIDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADVS 600
RIDD+ NTN RNGEC AAA+SH EEVHE S++S TSSCS+KD+N I TS NNLEN+ADVS
Sbjct: 541 RIDDDANTNLRNGECLAAANSHHEEVHEVSQVSNTSSCSIKDVNFIPTSTNNLENLADVS 600
Query: 601 SQNNGDYGLIGVNPSINIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDDF 660
SQNNGD+G +GVNPS N+ AADTS+CKEAEKLAIQKILSD YDSHQ FPCEPEEL+DF
Sbjct: 601 SQNNGDHGSMGVNPSKNVSDTAADTSNCKEAEKLAIQKILSDSYDSHQDFPCEPEELEDF 660
Query: 661 DYKNQAKDFGATKQGSPIILSSAANTSSVV-PIPSSNEARQSSCSYSNGGLEELEPAELV 720
DY QAKDFGATKQGSP ++SS ANTS VV P S NEARQSS SYSNGGLEELEPAE+V
Sbjct: 661 DYNKQAKDFGATKQGSP-MMSSVANTSPVVLPTVSCNEARQSSSSYSNGGLEELEPAEIV 720
Query: 721 APLSTGTAPAPAAGRKPIIRWLCNSL 745
APLSTGT AP A RKPIIR SL
Sbjct: 721 APLSTGT--APVAERKPIIRLSFTSL 740
BLAST of MS021666 vs. ExPASy TrEMBL
Match:
A0A1S3CFS4 (Polynucleotide adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103500202 PE=3 SV=1)
HSP 1 Score: 1301.6 bits (3367), Expect = 0.0e+00
Identity = 655/746 (87.80%), Postives = 689/746 (92.36%), Query Frame = 0
Query: 1 MGSPALSGRNNG-QRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEV 60
MGSPAL GRNNG QRLGITDPISLSGPTEYDV+KTRELEKYLQDAGLYESQEEAV+REEV
Sbjct: 1 MGSPALCGRNNGQQRLGITDPISLSGPTEYDVLKTRELEKYLQDAGLYESQEEAVNREEV 60
Query: 61 LGRLDQQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRH 120
LGRLD QIVKIWVKAISR+KGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRH
Sbjct: 61 LGRLD-QIVKIWVKAISRSKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRH 120
Query: 121 ATREEDFFGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDL 180
ATREEDFFGELHKMLSEMPEV+ELHPVPDAHVPVM+FK +GVSIDLLYAKLSLWVIPEDL
Sbjct: 121 ATREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMRFKLSGVSIDLLYAKLSLWVIPEDL 180
Query: 181 DISQDSILQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSG 240
DISQ+SILQN DEQTVRSLNGCRVTDR+LRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSG
Sbjct: 181 DISQESILQNTDEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSG 240
Query: 241 FLGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDP 300
FLGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGL VWDP
Sbjct: 241 FLGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLPVWDP 300
Query: 301 RRNPKDRFHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLF 360
RRNPKDR+HLMPIITPAYPCMNSSYNVS+STLRIMTEEFQRGHDICEVMEENKADWDTLF
Sbjct: 301 RRNPKDRYHLMPIITPAYPCMNSSYNVSASTLRIMTEEFQRGHDICEVMEENKADWDTLF 360
Query: 361 ESYPFFEAYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDF 420
E YPFFEAYKNYLQID+TAEN DD+R WKGWVESRLRQLTLKIERHTYNMLQCHP+PGDF
Sbjct: 361 EPYPFFEAYKNYLQIDITAENDDDIRIWKGWVESRLRQLTLKIERHTYNMLQCHPYPGDF 420
Query: 421 SDKSRPFHCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHV 480
SDKSRPFH CYFMGLQRKQGG P SG EQFDIRLTV+EFKRSVN+YT K GMEIYVSHV
Sbjct: 421 SDKSRPFHHCYFMGLQRKQGG-PASGSEQFDIRLTVDEFKRSVNVYTQRKRGMEIYVSHV 480
Query: 481 KRRNIPNFVFSGGVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRM 540
KRR+IPNFVF GGVRPSRASK+TWD RRSSELKASDSTQVD P E TES DG DDRRK++
Sbjct: 481 KRRSIPNFVFPGGVRPSRASKLTWDIRRSSELKASDSTQVDSPSEVTESLDG-DDRRKKI 540
Query: 541 RIDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADVS 600
RIDD+ NTN RNGEC AAA+SH EEVHE S++S TSSCS+KD+N I TS NNLEN+ADVS
Sbjct: 541 RIDDDANTNLRNGECLAAANSHHEEVHEVSQVSNTSSCSIKDVNFIPTSTNNLENLADVS 600
Query: 601 SQNNGDYGLIGVNPSINIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDDF 660
SQNNGD+G +GVNPS N+ AADTS+CKEAEKLAIQKILSD YDSHQ FPCEPEEL+DF
Sbjct: 601 SQNNGDHGSMGVNPSKNVSDTAADTSNCKEAEKLAIQKILSDSYDSHQDFPCEPEELEDF 660
Query: 661 DYKNQAKDFGATKQGSPIILSSAANTSSVV-PIPSSNEARQSSCSYSNGGLEELEPAELV 720
DY QAKDFGATKQGSP ++SS ANTS VV P S NEARQSS SYSNGGLEELEPAE+V
Sbjct: 661 DYNKQAKDFGATKQGSP-MMSSVANTSPVVLPTVSCNEARQSSSSYSNGGLEELEPAEIV 720
Query: 721 APLSTGTAPAPAAGRKPIIRWLCNSL 745
APLSTGT AP A RKPIIR SL
Sbjct: 721 APLSTGT--APVAERKPIIRLSFTSL 740
BLAST of MS021666 vs. ExPASy TrEMBL
Match:
A0A0A0LQF9 (Polynucleotide adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G042640 PE=3 SV=1)
HSP 1 Score: 1299.3 bits (3361), Expect = 0.0e+00
Identity = 656/747 (87.82%), Postives = 690/747 (92.37%), Query Frame = 0
Query: 1 MGSPALSGRNNG-QRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEV 60
MGSPAL GRNNG QRLGITDPISLSGPTEYDV+KTRELEKYLQDAGLYESQE+AV+REEV
Sbjct: 1 MGSPALCGRNNGQQRLGITDPISLSGPTEYDVLKTRELEKYLQDAGLYESQEDAVNREEV 60
Query: 61 LGRLDQQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRH 120
LGRLD QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRH
Sbjct: 61 LGRLD-QIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRH 120
Query: 121 ATREEDFFGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDL 180
ATREEDFFGELHKMLSEMPEV+ELHPVPDAHVPVM+FK +GVSIDLLYAKLSLWVIPEDL
Sbjct: 121 ATREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMRFKLSGVSIDLLYAKLSLWVIPEDL 180
Query: 181 DISQDSILQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSG 240
DISQDSILQN DEQTVRSLNGCRVTDR+LRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSG
Sbjct: 181 DISQDSILQNTDEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSG 240
Query: 241 FLGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDP 300
FLGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDP
Sbjct: 241 FLGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDP 300
Query: 301 RRNPKDRFHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLF 360
RRNPKDR+HLMPIITPAYPCMNSSYNVS+STLRIMTEEF+RGHDICEVMEENK+DWDTLF
Sbjct: 301 RRNPKDRYHLMPIITPAYPCMNSSYNVSASTLRIMTEEFRRGHDICEVMEENKSDWDTLF 360
Query: 361 ESYPFFEAYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDF 420
E YPFFEAYKNYLQID+TAEN DD+R WKGWVESRLRQLTLKIERHTYNMLQCHP+PGDF
Sbjct: 361 EPYPFFEAYKNYLQIDITAENDDDIRIWKGWVESRLRQLTLKIERHTYNMLQCHPYPGDF 420
Query: 421 SDKSRPFHCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHV 480
SDKSRPFH CYFMGLQRKQGG P SGGEQFDIRLTV+EFK SVN+YT K GMEIYVSHV
Sbjct: 421 SDKSRPFHHCYFMGLQRKQGG-PASGGEQFDIRLTVDEFKHSVNVYTQRKRGMEIYVSHV 480
Query: 481 KRRNIPNFVFSGGVRPSRASKVTWDSRRSSELKASDSTQVDRPFEATESSDGVDDRRKRM 540
KRR+IPNFVF GGVRPSRASK+TWD RRSSELKASDSTQVD P EATES DG DDRRKR+
Sbjct: 481 KRRSIPNFVFPGGVRPSRASKLTWDIRRSSELKASDSTQVDSPSEATESLDG-DDRRKRI 540
Query: 541 RIDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADVS 600
RIDDN NTN RNGEC A AHSH EEVHE S++S TSSCS+KD+N I TSANNLEN+ADVS
Sbjct: 541 RIDDNANTNLRNGECLAMAHSHPEEVHEVSQVSNTSSCSIKDVNFIPTSANNLENLADVS 600
Query: 601 SQNNGDYGLIGVNPSI-NIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDD 660
SQNNGD+G + V+PS N+ AAADTS+CKEAEKLAIQKILSD YDSHQ FPCE EEL+D
Sbjct: 601 SQNNGDHGSLRVSPSTNNVSDAAADTSNCKEAEKLAIQKILSDSYDSHQDFPCETEELED 660
Query: 661 FDYKNQAKDFGATKQGSPIILSSAANTSS-VVPIPSSNEARQSSCSYSNGGLEELEPAEL 720
FDY NQAKDFGATKQGSP ++SS ANTS V+P S NEARQSS SY NGGLEELEPAE+
Sbjct: 661 FDYNNQAKDFGATKQGSP-MMSSVANTSPLVLPTVSCNEARQSSSSYYNGGLEELEPAEI 720
Query: 721 VAPLSTGTAPAPAAGRKPIIRWLCNSL 745
VAPLSTGT AP A RKPIIR SL
Sbjct: 721 VAPLSTGT--APVAERKPIIRLSFTSL 741
BLAST of MS021666 vs. TAIR 10
Match:
AT1G17980.1 (poly(A) polymerase 1 )
HSP 1 Score: 865.1 bits (2234), Expect = 4.0e-251
Identity = 467/748 (62.43%), Postives = 543/748 (72.59%), Query Frame = 0
Query: 7 SGRNNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQQ 66
S + NGQR G+++PIS+ GPTE+DVIKTRELEK+LQD GLYES+EEAV REEVLG LD Q
Sbjct: 3 SVQQNGQRFGVSEPISMGGPTEFDVIKTRELEKHLQDVGLYESKEEAVRREEVLGILD-Q 62
Query: 67 IVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDF 126
IVK W+K ISRAKGLN+QL+ EANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATRE DF
Sbjct: 63 IVKTWIKTISRAKGLNDQLLHEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREGDF 122
Query: 127 FGELHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDISQDSI 186
FGEL +MLSEMPEVTELHPVPDAHVP+M FK GVSIDLLYA+L LWVIPEDLD+SQDSI
Sbjct: 123 FGELQRMLSEMPEVTELHPVPDAHVPLMGFKLNGVSIDLLYAQLPLWVIPEDLDLSQDSI 182
Query: 187 LQNADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINW 246
LQNADEQTVRSLNGCRVTD++LRLVPNIQ+FRTTLRCMRFWAKRRGVYSNVSGFLGGINW
Sbjct: 183 LQNADEQTVRSLNGCRVTDQILRLVPNIQNFRTTLRCMRFWAKRRGVYSNVSGFLGGINW 242
Query: 247 ALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPRRNPKDR 306
ALLVARICQLYPNALPN+LVSRFFRVF QW WPN + LC+ +EGSLGLQVWDPR NPKDR
Sbjct: 243 ALLVARICQLYPNALPNILVSRFFRVFYQWNWPNAIFLCSPDEGSLGLQVWDPRINPKDR 302
Query: 307 FHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFESYPFFE 366
H+MPIITPAYPCMNSSYNVS STLRIM EFQRG++ICE ME NKADWDTLFE + FFE
Sbjct: 303 LHIMPIITPAYPCMNSSYNVSESTLRIMKGEFQRGNEICEAMESNKADWDTLFEPFAFFE 362
Query: 367 AYKNYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFSDKSRPF 426
AYKNYLQID++A NVDDLRKWKGWVESRLRQLTLKIERH + ML CHPHP DF D SRP
Sbjct: 363 AYKNYLQIDISAANVDDLRKWKGWVESRLRQLTLKIERH-FKMLHCHPHPHDFQDTSRPL 422
Query: 427 HCCYFMGLQRKQGGAPTSGGEQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVKRRNIPN 486
HC YFMGLQRKQ G P + GEQFDIR TVEEFK +VN YTLW PGMEI V H+KRR++PN
Sbjct: 423 HCSYFMGLQRKQ-GVPAAEGEQFDIRRTVEEFKHTVNAYTLWIPGMEISVGHIKRRSLPN 482
Query: 487 FVFSGGVRPSRASKVTWDSRRSSELK--------ASDSTQVDRPFEATESSDGVDDRRKR 546
FVF GGVRPS SK TWDS R SE + A+ +T + E+ S+ D +KR
Sbjct: 483 FVFPGGVRPSHTSKGTWDSNRRSEHRNSSTSSAPAATTTTTEMSSESKAGSNSPVDGKKR 542
Query: 547 MRIDDNVNTNFRNGECSAAAHSHTEEVHEASRISITSSCSVKDMNSIHTSANNLENMADV 606
D + + T++ + I+++ + S + S ++ +
Sbjct: 543 KWGD---------------SETLTDQPRNSKHIAVSVPVENCEGGSPNPSVGSICSSPMK 602
Query: 607 SSQNNGDYGLIGVNPSINIESAAADTSSCKEAEKLAIQKILSDPYDSHQAFPCEPEELDD 666
NG I +P N+ + + D E L I+KI + +H E EEL++
Sbjct: 603 DYCTNGKSEPISKDPPENVVAFSKD-----PPESLPIEKIATP--QAH-----ETEELEE 662
Query: 667 -FDYKNQAKDFGATKQGSPIILSSAANTSSVVPIPSSNEARQSSCSYSNGGLEELEPAEL 726
FD+ NQ + + K +LS+ T+++ P EA + + +EELE
Sbjct: 663 SFDFGNQVIEQISHKVA---VLSA---TATIPPF----EATSNGSPFPYEAVEELEVLPT 707
Query: 727 VAPLSTGTAPAPAA-GRKPIIRWLCNSL 745
P A P+ RKPII+ SL
Sbjct: 723 RQP---DAAHRPSVQQRKPIIKLSFTSL 707
BLAST of MS021666 vs. TAIR 10
Match:
AT4G32850.2 (nuclear poly(a) polymerase )
HSP 1 Score: 704.5 bits (1817), Expect = 9.0e-203
Identity = 329/495 (66.46%), Postives = 400/495 (80.81%), Query Frame = 0
Query: 10 NNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQQIVK 69
N+ + GIT P+SL+GP+ D+ + ELEKYL D GLYES+++ + REEVLGR+D QIVK
Sbjct: 16 NSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMRREEVLGRID-QIVK 75
Query: 70 IWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGE 129
WVK +++ +G +Q+V++ANA IFTFGSYRLGVHGPGADIDTLCVGP + REEDFF
Sbjct: 76 HWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNREEDFFII 135
Query: 130 LHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDISQDSILQN 189
LH +L+EM EVTELHPVPDAHVPVMKFKF G+ IDLLYA +SL V+P+DLDIS S+L
Sbjct: 136 LHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVPQDLDISSSSVLCE 195
Query: 190 ADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL 249
DE TVRSLNGCRV D++L+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALL
Sbjct: 196 VDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALL 255
Query: 250 VARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPRRNPKDRFHL 309
VAR+CQLYPNA+P+MLVSRFFRV+TQWRWPNPVMLCA EE LG VWD R+N +DR+HL
Sbjct: 256 VARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPVWDRRKNHRDRYHL 315
Query: 310 MPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFESYPFFEAYK 369
MPIITPAYPCMNSSYNVS STLR+MTE+FQ G++I + +E NK W +LFE Y FFEAYK
Sbjct: 316 MPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWSSLFEQYMFFEAYK 375
Query: 370 NYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFSDKSRPF-HC 429
NYLQ+D+ A + +DL WKGWVESR RQLTLKIER T ML CHP P ++ D +R F HC
Sbjct: 376 NYLQVDIVAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTARQFLHC 435
Query: 430 CYFMGLQRKQGGAPTSGG---EQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVKRRNIP 489
+FMGLQR +G GG +QFDIR TV+EF++ VNMY WKPGM+++VSHV+RR +P
Sbjct: 436 AFFMGLQRAEG----VGGQECQQFDIRGTVDEFRQEVNMYMFWKPGMDVFVSHVRRRQLP 495
Query: 490 NFVFSGGVRPSRASK 501
FVF G R R S+
Sbjct: 496 PFVFPNGYRRPRQSR 505
BLAST of MS021666 vs. TAIR 10
Match:
AT4G32850.3 (nuclear poly(a) polymerase )
HSP 1 Score: 704.5 bits (1817), Expect = 9.0e-203
Identity = 329/495 (66.46%), Postives = 400/495 (80.81%), Query Frame = 0
Query: 10 NNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQQIVK 69
N+ + GIT P+SL+GP+ D+ + ELEKYL D GLYES+++ + REEVLGR+D QIVK
Sbjct: 16 NSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMRREEVLGRID-QIVK 75
Query: 70 IWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGE 129
WVK +++ +G +Q+V++ANA IFTFGSYRLGVHGPGADIDTLCVGP + REEDFF
Sbjct: 76 HWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNREEDFFII 135
Query: 130 LHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDISQDSILQN 189
LH +L+EM EVTELHPVPDAHVPVMKFKF G+ IDLLYA +SL V+P+DLDIS S+L
Sbjct: 136 LHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVPQDLDISSSSVLCE 195
Query: 190 ADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL 249
DE TVRSLNGCRV D++L+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALL
Sbjct: 196 VDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALL 255
Query: 250 VARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPRRNPKDRFHL 309
VAR+CQLYPNA+P+MLVSRFFRV+TQWRWPNPVMLCA EE LG VWD R+N +DR+HL
Sbjct: 256 VARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPVWDRRKNHRDRYHL 315
Query: 310 MPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFESYPFFEAYK 369
MPIITPAYPCMNSSYNVS STLR+MTE+FQ G++I + +E NK W +LFE Y FFEAYK
Sbjct: 316 MPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWSSLFEQYMFFEAYK 375
Query: 370 NYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFSDKSRPF-HC 429
NYLQ+D+ A + +DL WKGWVESR RQLTLKIER T ML CHP P ++ D +R F HC
Sbjct: 376 NYLQVDIVAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTARQFLHC 435
Query: 430 CYFMGLQRKQGGAPTSGG---EQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVKRRNIP 489
+FMGLQR +G GG +QFDIR TV+EF++ VNMY WKPGM+++VSHV+RR +P
Sbjct: 436 AFFMGLQRAEG----VGGQECQQFDIRGTVDEFRQEVNMYMFWKPGMDVFVSHVRRRQLP 495
Query: 490 NFVFSGGVRPSRASK 501
FVF G R R S+
Sbjct: 496 PFVFPNGYRRPRQSR 505
BLAST of MS021666 vs. TAIR 10
Match:
AT4G32850.6 (nuclear poly(a) polymerase )
HSP 1 Score: 704.5 bits (1817), Expect = 9.0e-203
Identity = 329/495 (66.46%), Postives = 400/495 (80.81%), Query Frame = 0
Query: 10 NNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQQIVK 69
N+ + GIT P+SL+GP+ D+ + ELEKYL D GLYES+++ + REEVLGR+D QIVK
Sbjct: 16 NSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMRREEVLGRID-QIVK 75
Query: 70 IWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGE 129
WVK +++ +G +Q+V++ANA IFTFGSYRLGVHGPGADIDTLCVGP + REEDFF
Sbjct: 76 HWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNREEDFFII 135
Query: 130 LHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDISQDSILQN 189
LH +L+EM EVTELHPVPDAHVPVMKFKF G+ IDLLYA +SL V+P+DLDIS S+L
Sbjct: 136 LHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVPQDLDISSSSVLCE 195
Query: 190 ADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL 249
DE TVRSLNGCRV D++L+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALL
Sbjct: 196 VDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALL 255
Query: 250 VARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPRRNPKDRFHL 309
VAR+CQLYPNA+P+MLVSRFFRV+TQWRWPNPVMLCA EE LG VWD R+N +DR+HL
Sbjct: 256 VARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPVWDRRKNHRDRYHL 315
Query: 310 MPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFESYPFFEAYK 369
MPIITPAYPCMNSSYNVS STLR+MTE+FQ G++I + +E NK W +LFE Y FFEAYK
Sbjct: 316 MPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWSSLFEQYMFFEAYK 375
Query: 370 NYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFSDKSRPF-HC 429
NYLQ+D+ A + +DL WKGWVESR RQLTLKIER T ML CHP P ++ D +R F HC
Sbjct: 376 NYLQVDIVAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTARQFLHC 435
Query: 430 CYFMGLQRKQGGAPTSGG---EQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVKRRNIP 489
+FMGLQR +G GG +QFDIR TV+EF++ VNMY WKPGM+++VSHV+RR +P
Sbjct: 436 AFFMGLQRAEG----VGGQECQQFDIRGTVDEFRQEVNMYMFWKPGMDVFVSHVRRRQLP 495
Query: 490 NFVFSGGVRPSRASK 501
FVF G R R S+
Sbjct: 496 PFVFPNGYRRPRQSR 505
BLAST of MS021666 vs. TAIR 10
Match:
AT4G32850.5 (nuclear poly(a) polymerase )
HSP 1 Score: 704.5 bits (1817), Expect = 9.0e-203
Identity = 329/495 (66.46%), Postives = 400/495 (80.81%), Query Frame = 0
Query: 10 NNGQRLGITDPISLSGPTEYDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQQIVK 69
N+ + GIT P+SL+GP+ D+ + ELEKYL D GLYES+++ + REEVLGR+D QIVK
Sbjct: 16 NSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMRREEVLGRID-QIVK 75
Query: 70 IWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGE 129
WVK +++ +G +Q+V++ANA IFTFGSYRLGVHGPGADIDTLCVGP + REEDFF
Sbjct: 76 HWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNREEDFFII 135
Query: 130 LHKMLSEMPEVTELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDISQDSILQN 189
LH +L+EM EVTELHPVPDAHVPVMKFKF G+ IDLLYA +SL V+P+DLDIS S+L
Sbjct: 136 LHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVPQDLDISSSSVLCE 195
Query: 190 ADEQTVRSLNGCRVTDRVLRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL 249
DE TVRSLNGCRV D++L+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALL
Sbjct: 196 VDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALL 255
Query: 250 VARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPRRNPKDRFHL 309
VAR+CQLYPNA+P+MLVSRFFRV+TQWRWPNPVMLCA EE LG VWD R+N +DR+HL
Sbjct: 256 VARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPVWDRRKNHRDRYHL 315
Query: 310 MPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFESYPFFEAYK 369
MPIITPAYPCMNSSYNVS STLR+MTE+FQ G++I + +E NK W +LFE Y FFEAYK
Sbjct: 316 MPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWSSLFEQYMFFEAYK 375
Query: 370 NYLQIDVTAENVDDLRKWKGWVESRLRQLTLKIERHTYNMLQCHPHPGDFSDKSRPF-HC 429
NYLQ+D+ A + +DL WKGWVESR RQLTLKIER T ML CHP P ++ D +R F HC
Sbjct: 376 NYLQVDIVAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTARQFLHC 435
Query: 430 CYFMGLQRKQGGAPTSGG---EQFDIRLTVEEFKRSVNMYTLWKPGMEIYVSHVKRRNIP 489
+FMGLQR +G GG +QFDIR TV+EF++ VNMY WKPGM+++VSHV+RR +P
Sbjct: 436 AFFMGLQRAEG----VGGQECQQFDIRGTVDEFRQEVNMYMFWKPGMDVFVSHVRRRQLP 495
Query: 490 NFVFSGGVRPSRASK 501
FVF G R R S+
Sbjct: 496 PFVFPNGYRRPRQSR 505
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022954916.1 | 0.0e+00 | 90.73 | nuclear poly(A) polymerase 1-like [Cucurbita moschata] | [more] |
| KAG6572954.1 | 0.0e+00 | 90.32 | Nuclear poly(A) polymerase 1, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_023541715.1 | 0.0e+00 | 90.19 | nuclear poly(A) polymerase 1-like [Cucurbita pepo subsp. pepo] | [more] |
| XP_022994133.1 | 0.0e+00 | 90.05 | nuclear poly(A) polymerase 1-like [Cucurbita maxima] | [more] |
| KAG7012136.1 | 0.0e+00 | 86.47 | Nuclear poly(A) polymerase 1 [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9LMT2 | 5.6e-250 | 62.43 | Nuclear poly(A) polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=PAPS1 PE=1 SV=1 | [more] |
| Q8VYW1 | 1.3e-201 | 66.46 | Nuclear poly(A) polymerase 4 OS=Arabidopsis thaliana OX=3702 GN=PAPS4 PE=1 SV=1 | [more] |
| O82312 | 1.9e-197 | 60.35 | Nuclear poly(A) polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=PAPS2 PE=1 SV=2 | [more] |
| Q54J73 | 2.3e-126 | 42.90 | Poly(A) polymerase OS=Dictyostelium discoideum OX=44689 GN=papA PE=3 SV=1 | [more] |
| P51003 | 1.3e-121 | 44.47 | Poly(A) polymerase alpha OS=Homo sapiens OX=9606 GN=PAPOLA PE=1 SV=4 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1GS98 | 0.0e+00 | 90.73 | Polynucleotide adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111457031... | [more] |
| A0A6J1K4A6 | 0.0e+00 | 90.05 | Polynucleotide adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111489961 P... | [more] |
| A0A5D3BLG8 | 0.0e+00 | 87.80 | Polynucleotide adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... | [more] |
| A0A1S3CFS4 | 0.0e+00 | 87.80 | Polynucleotide adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103500202 PE=3 ... | [more] |
| A0A0A0LQF9 | 0.0e+00 | 87.82 | Polynucleotide adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G042640 PE... | [more] |
