MS021662 (gene) Bitter gourd (TR) v1

Overview


Name	MS021662
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	E3 ubiquitin-protein ligase MBR2 isoform X2
Location	scaffold348: 156714 .. 158899 (+)
RNA-Seq Expression	MS021662
Synteny	MS021662

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTGTTGTTACACAGACTACTACAGTGGGTGAACACATCAAATTGAGAAGACCCAGAGGCCATTTGAGCCAACCCATTTCAGAATCAGATCCAAACCCATCAATCCCTTCGATAATTCAATCCACTCGCTGCAAATCCACCATTTCCTCTCTCCTTCTCTCGACCTTTTCCAATAGCGGCGCCACTGAATCTTTACCCTCTTCAAGTGTCACGAATAGGAAGAAGAGCAACTTCTCTTCTGCAACTTTCAGGGGACTCGGATGCACTGCCTCCGCTTCTCAGCAGGTATCGGTCCCGGCTGTAATTCGAACTTCCGCGGATTGGCAGAAGAAGAAGACGAGGAAAAAAAAGCAGAAGAGCTCCAAGAACAAAACCCAGCAAGGAATTGGTGATGGAACTTATTTTCACCCAAATTCGAATTTCAATTCTGCTAGCTGTTTAGACGCTCAAGATGTTTGGTGCGGTCCTGGAATTGGATTCTCTCCTGATGCTGCTGCTTCTGTGGATTGCGTTGTCGCGAGAAGGCATGCTTCTGGGAGGGGAAAAATTGATGTGGAGAAGATGAGCCAGAGGGAGGTAACTCGCTTTTCTATTTCTTCTTAACATTCTTGAACATCTCTCTTCTATGGTTGTGGGTTAGGCATTCGTTGGTAAAGAAACTTGAGATGTTTATTTTGTTCTGCTTTAGTCATCTTATTACTAGTTCTTCAGATATGGGCTCTTACTGAACTTCCTCGGCTTGGTTTGTTTTCTTGCCTGCAATATGGTTCTAAATCAGTTTGAACCTGTTCAACTTCCATTCTTGGAACTTGCTCCCTTTTCATTCTTCTTTGTAAATTTGAATATTTAATGCAAATGGGTATGCATTAAATTTGAAGGTCCTGCAAATGAAGTCCTCCTTTGGGGCTATAAAAATGAGAAACTCTTGTTCTTGTTTAATGTGTTATAGTTTTAACTTCCTCTGCTTCTACCATCGCCAGCGTTCTTGCTTAGGAAGGCGAACAGTGAACCCCGAGACCCTCTTGTTTTTGGATTCTGATTCTGATCTTCCAACAGCACGGTCATTGGAAGTGTCTCGGAGTCGGTATTATCGCCATGTTCGGCATCCATCCCCGGACGGGCTGGCTGAGGTGAGCCATCATTCATATTTGTACCCAATGCCATAAATATAACTATATAAGCTTCAGTGTTTTATTTTTCTGTTCTTTATTTGCTTGAAATGGTTTGGCAGATCACATTTGGATGTCTTGGTAAGGTTCCGATATTTGAAAAATTAGATATTCTGCTTTTATGAGATGGTTTGGCAGATGGCATCGGCTCAAAGAAACAATTTGAACTTGCTTACAAGCTAATCTGTTTTGCTAGATTACGTTTCCTATTTGGAATGTCTTTGATCTCATCCATTGATTCCAACTGCAAGTTTATAGTTAATGATAGGAATCATGGAAGAAAGCATAGATTAATGTTGCAATATACACCATATTTTGAATCATATAGTGTAAATTTTATCATGTTTCTGTATTGTCACTCCCTCCCTCCCTTGTTTCATAATGATCATATAATACGGGTATGCTCACTTGATATATTATTTATAGCAGATTATGATGTTCCAGAGCAGTTTGCTGATGGGTGGAAGGTTTGATACTCACGATCAATTTAGGGAATTGCGGCTCGATGTTGATAACATGTCATATGAGGTTCATAACTTTCACCAATTCCTTCTACCTCTTCTTTCCTGCAACAATCTGATGTTAACCCATCTTTGTTATCTCGAGATGGAAACAGGAGCTGCTTGAACTCGGTGAGAGGATTGGTCATGTGAGTACCGGGCTGAAAGAAGACGAGATCGGCAAATGTATTAGGAAGATGAAGCCTTTTATCGTAAATGAGCTAGCATCCCATTTATTATCTCAAGTAGATCGGAAGTGCAGTATCTGCCAGGTAAAGTTGTAGTTACTCGAGTCCAGCTTAGTGGTTTTGATTAGAACTCATATATGTAATTGTTCTTTCCGACCTCATTCTTGACACCTAAAATCATTTTGTACAAGCAGGAGGACTATGAAGTAGACGATGAAATGGGTAAGCTGGAATGTGGGCACAGCTATCACATACACTGTATAAAACAGTGGCTTGCACAGAAGAATACGTGCCCGGTCTGTAAGACAGCAGCGGTGGGCCGAGGC

mRNA sequence

ATGCCTGTTGTTACACAGACTACTACAGTGGGTGAACACATCAAATTGAGAAGACCCAGAGGCCATTTGAGCCAACCCATTTCAGAATCAGATCCAAACCCATCAATCCCTTCGATAATTCAATCCACTCGCTGCAAATCCACCATTTCCTCTCTCCTTCTCTCGACCTTTTCCAATAGCGGCGCCACTGAATCTTTACCCTCTTCAAGTGTCACGAATAGGAAGAAGAGCAACTTCTCTTCTGCAACTTTCAGGGGACTCGGATGCACTGCCTCCGCTTCTCAGCAGGTATCGGTCCCGGCTGTAATTCGAACTTCCGCGGATTGGCAGAAGAAGAAGACGAGGAAAAAAAAGCAGAAGAGCTCCAAGAACAAAACCCAGCAAGGAATTGGTGATGGAACTTATTTTCACCCAAATTCGAATTTCAATTCTGCTAGCTGTTTAGACGCTCAAGATGTTTGGTGCGGTCCTGGAATTGGATTCTCTCCTGATGCTGCTGCTTCTGTGGATTGCGTTGTCGCGAGAAGGCATGCTTCTGGGAGGGGAAAAATTGATGTGGAGAAGATGAGCCAGAGGGAGCGTTCTTGCTTAGGAAGGCGAACAGTGAACCCCGAGACCCTCTTGTTTTTGGATTCTGATTCTGATCTTCCAACAGCACGGTCATTGGAAGTGTCTCGGAGTCGGTATTATCGCCATGTTCGGCATCCATCCCCGGACGGGCTGGCTGAGATTATGATGTTCCAGAGCAGTTTGCTGATGGGTGGAAGGTTTGATACTCACGATCAATTTAGGGAATTGCGGCTCGATGTTGATAACATGTCATATGAGGAGCTGCTTGAACTCGGTGAGAGGATTGGTCATGTGAGTACCGGGCTGAAAGAAGACGAGATCGGCAAATGTATTAGGAAGATGAAGCCTTTTATCGTAAATGAGCTAGCATCCCATTTATTATCTCAAGTAGATCGGAAGTGCAGTATCTGCCAGCAGGAGGACTATGAAGTAGACGATGAAATGGGTAAGCTGGAATGTGGGCACAGCTATCACATACACTGTATAAAACAGTGGCTTGCACAGAAGAATACGTGCCCGGTCTGTAAGACAGCAGCGGTGGGCCGAGGC

Coding sequence (CDS)

ATGCCTGTTGTTACACAGACTACTACAGTGGGTGAACACATCAAATTGAGAAGACCCAGAGGCCATTTGAGCCAACCCATTTCAGAATCAGATCCAAACCCATCAATCCCTTCGATAATTCAATCCACTCGCTGCAAATCCACCATTTCCTCTCTCCTTCTCTCGACCTTTTCCAATAGCGGCGCCACTGAATCTTTACCCTCTTCAAGTGTCACGAATAGGAAGAAGAGCAACTTCTCTTCTGCAACTTTCAGGGGACTCGGATGCACTGCCTCCGCTTCTCAGCAGGTATCGGTCCCGGCTGTAATTCGAACTTCCGCGGATTGGCAGAAGAAGAAGACGAGGAAAAAAAAGCAGAAGAGCTCCAAGAACAAAACCCAGCAAGGAATTGGTGATGGAACTTATTTTCACCCAAATTCGAATTTCAATTCTGCTAGCTGTTTAGACGCTCAAGATGTTTGGTGCGGTCCTGGAATTGGATTCTCTCCTGATGCTGCTGCTTCTGTGGATTGCGTTGTCGCGAGAAGGCATGCTTCTGGGAGGGGAAAAATTGATGTGGAGAAGATGAGCCAGAGGGAGCGTTCTTGCTTAGGAAGGCGAACAGTGAACCCCGAGACCCTCTTGTTTTTGGATTCTGATTCTGATCTTCCAACAGCACGGTCATTGGAAGTGTCTCGGAGTCGGTATTATCGCCATGTTCGGCATCCATCCCCGGACGGGCTGGCTGAGATTATGATGTTCCAGAGCAGTTTGCTGATGGGTGGAAGGTTTGATACTCACGATCAATTTAGGGAATTGCGGCTCGATGTTGATAACATGTCATATGAGGAGCTGCTTGAACTCGGTGAGAGGATTGGTCATGTGAGTACCGGGCTGAAAGAAGACGAGATCGGCAAATGTATTAGGAAGATGAAGCCTTTTATCGTAAATGAGCTAGCATCCCATTTATTATCTCAAGTAGATCGGAAGTGCAGTATCTGCCAGCAGGAGGACTATGAAGTAGACGATGAAATGGGTAAGCTGGAATGTGGGCACAGCTATCACATACACTGTATAAAACAGTGGCTTGCACAGAAGAATACGTGCCCGGTCTGTAAGACAGCAGCGGTGGGCCGAGGC

Protein sequence

MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNSGATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIKQWLAQKNTCPVCKTAAVGRG

Homology

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BLAST of MS021662 vs. NCBI nr
Match: XP_022137196.1 (uncharacterized protein LOC111008721 isoform X2 [Momordica charantia])

HSP 1 Score: 732.6 bits (1890), Expect = 1.7e-207
Identity = 370/373 (99.20%), Postives = 370/373 (99.20%), Query Frame = 0


Query: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS 60
           MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS
Sbjct: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS 60

Query: 61  GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK 120
           GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK
Sbjct: 61  GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK 120

Query: 121 SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG 180
           SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG
Sbjct: 121 SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG 180

Query: 181 RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG 240
           RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG
Sbjct: 181 RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG 240

Query: 241 LAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKC 300
           LAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKC
Sbjct: 241 LAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKC 300

Query: 301 IRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIKQWLAQKN 360
           IRKMKPFIVNELASHLLSQVDRKCSIC QEDYEVDDEMGKLECGHSYHIHCIKQWLAQKN
Sbjct: 301 IRKMKPFIVNELASHLLSQVDRKCSIC-QEDYEVDDEMGKLECGHSYHIHCIKQWLAQKN 360

Query: 361 TCPVCKTAAVGRG 374
            CPVCKTA VGRG
Sbjct: 361 MCPVCKTAVVGRG 372

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BLAST of MS021662 vs. NCBI nr
Match: XP_022137195.1 (uncharacterized protein LOC111008721 isoform X1 [Momordica charantia])

HSP 1 Score: 728.0 bits (1878), Expect = 4.1e-206
Identity = 370/374 (98.93%), Postives = 370/374 (98.93%), Query Frame = 0


Query: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS 60
           MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS
Sbjct: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS 60

Query: 61  GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK 120
           GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK
Sbjct: 61  GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK 120

Query: 121 SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG 180
           SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG
Sbjct: 121 SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG 180

Query: 181 RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG 240
           RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG
Sbjct: 181 RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG 240

Query: 241 LAE-IMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGK 300
           LAE IMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGK
Sbjct: 241 LAEQIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGK 300

Query: 301 CIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIKQWLAQK 360
           CIRKMKPFIVNELASHLLSQVDRKCSIC QEDYEVDDEMGKLECGHSYHIHCIKQWLAQK
Sbjct: 301 CIRKMKPFIVNELASHLLSQVDRKCSIC-QEDYEVDDEMGKLECGHSYHIHCIKQWLAQK 360

Query: 361 NTCPVCKTAAVGRG 374
           N CPVCKTA VGRG
Sbjct: 361 NMCPVCKTAVVGRG 373

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BLAST of MS021662 vs. NCBI nr
Match: XP_038894454.1 (E3 ubiquitin-protein ligase MBR2 isoform X2 [Benincasa hispida])

HSP 1 Score: 656.8 bits (1693), Expect = 1.2e-184
Identity = 334/380 (87.89%), Postives = 356/380 (93.68%), Query Frame = 0


Query: 1   MPVVTQTTTVGEHIKLRRPR---GHLSQPISESDPNP-SIPSIIQSTRCKSTISSLLLST 60
           MPVVT T+TVGEH+KLRRP+    HL+QPISESDPNP SIPSIIQSTRCKSTISSLLLST
Sbjct: 1   MPVVTHTSTVGEHMKLRRPKSILNHLNQPISESDPNPSSIPSIIQSTRCKSTISSLLLST 60

Query: 61  FSN---SGATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKK 120
           FSN   SG  E+LPSS +TNRKK+NFSSAT RGLGCT +ASQQVSVPAVIRTSADW++KK
Sbjct: 61  FSNNTTSGGNEALPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWERKK 120

Query: 121 TRKKKQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVV 180
           TRKKKQK+SKNKTQQGI D ++F PNSN NSASCLDAQDVWCGPGIGFS DAAASVDCVV
Sbjct: 121 TRKKKQKNSKNKTQQGIVDASHFQPNSNINSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 ARRHASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHV 240
           ARRHASGRGKID+EK++QRERSCLGRRTVNPETLLFLDSDSDLPTARSLE+SRSRYYRHV
Sbjct: 181 ARRHASGRGKIDLEKITQRERSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHV 240

Query: 241 RHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 300
           RHPSPDGLAEIMMFQSSLLMGGRFD HDQFRELRLDVDNMSYEELLELGERIGHVSTGLK
Sbjct: 241 RHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 300

Query: 301 EDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIK 360
           EDEI +CIRKMKP +VNELA+HLLSQ+DRKCSIC QEDYE DDEMGKLECGHSYHIHCIK
Sbjct: 301 EDEIVRCIRKMKPLVVNELATHLLSQMDRKCSIC-QEDYEPDDEMGKLECGHSYHIHCIK 360

Query: 361 QWLAQKNTCPVCKTAAVGRG 374
           QWL+QKNTCPVCKTAAVGRG
Sbjct: 361 QWLSQKNTCPVCKTAAVGRG 379

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BLAST of MS021662 vs. NCBI nr
Match: XP_038894453.1 (E3 ubiquitin-protein ligase MBR2 isoform X1 [Benincasa hispida])

HSP 1 Score: 652.1 bits (1681), Expect = 2.8e-183
Identity = 334/381 (87.66%), Postives = 356/381 (93.44%), Query Frame = 0


Query: 1   MPVVTQTTTVGEHIKLRRPR---GHLSQPISESDPNP-SIPSIIQSTRCKSTISSLLLST 60
           MPVVT T+TVGEH+KLRRP+    HL+QPISESDPNP SIPSIIQSTRCKSTISSLLLST
Sbjct: 1   MPVVTHTSTVGEHMKLRRPKSILNHLNQPISESDPNPSSIPSIIQSTRCKSTISSLLLST 60

Query: 61  FSN---SGATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKK 120
           FSN   SG  E+LPSS +TNRKK+NFSSAT RGLGCT +ASQQVSVPAVIRTSADW++KK
Sbjct: 61  FSNNTTSGGNEALPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWERKK 120

Query: 121 TRKKKQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVV 180
           TRKKKQK+SKNKTQQGI D ++F PNSN NSASCLDAQDVWCGPGIGFS DAAASVDCVV
Sbjct: 121 TRKKKQKNSKNKTQQGIVDASHFQPNSNINSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 ARRHASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHV 240
           ARRHASGRGKID+EK++QRERSCLGRRTVNPETLLFLDSDSDLPTARSLE+SRSRYYRHV
Sbjct: 181 ARRHASGRGKIDLEKITQRERSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRYYRHV 240

Query: 241 RHPSPDGLAE-IMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300
           RHPSPDGLAE IMMFQSSLLMGGRFD HDQFRELRLDVDNMSYEELLELGERIGHVSTGL
Sbjct: 241 RHPSPDGLAEQIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300

Query: 301 KEDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCI 360
           KEDEI +CIRKMKP +VNELA+HLLSQ+DRKCSIC QEDYE DDEMGKLECGHSYHIHCI
Sbjct: 301 KEDEIVRCIRKMKPLVVNELATHLLSQMDRKCSIC-QEDYEPDDEMGKLECGHSYHIHCI 360

Query: 361 KQWLAQKNTCPVCKTAAVGRG 374
           KQWL+QKNTCPVCKTAAVGRG
Sbjct: 361 KQWLSQKNTCPVCKTAAVGRG 380

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BLAST of MS021662 vs. NCBI nr
Match: XP_004137488.1 (E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis sativus])

HSP 1 Score: 646.7 bits (1667), Expect = 1.2e-181
Identity = 332/380 (87.37%), Postives = 353/380 (92.89%), Query Frame = 0


Query: 1   MPVVTQTTTVGEHIKLRRPR---GHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60
           MPVV    TVGEH+KLRRPR    HL+QPISESDPNPSIPSIIQSTRCKSTISSLLLSTF
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60

Query: 61  SN---SGATESLPSSSV-TNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKK 120
           SN   SG  ESLPSS + TNRKK+NFSSAT RGLGCT +ASQQVSVPAVIRTSADW+KKK
Sbjct: 61  SNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKK 120

Query: 121 TRKKKQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVV 180
           TRKKKQKSSKNKTQQGI D ++F PNS+ NSASCLDAQDVWCGPGIGFS DAAASVDCVV
Sbjct: 121 TRKKKQKSSKNKTQQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 ARRHASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHV 240
           ARRHASGRGKID+EK++QRERSCLGRRTV+PETLLFLDSDS++PTARSLE+SRSRYYRHV
Sbjct: 181 ARRHASGRGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSEIPTARSLELSRSRYYRHV 240

Query: 241 RHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 300
           RHPSPDGLAEIMMFQSSLLMGGRFD HDQFRELRLDVDNMSYEELLELGERIGHVSTGLK
Sbjct: 241 RHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 300

Query: 301 EDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIK 360
           +DEIG+CIRKMKP +VNEL +HLLSQ+DRKCSIC QEDYE DDEMGKLECGHSYHIHCIK
Sbjct: 301 DDEIGRCIRKMKPHVVNELTTHLLSQMDRKCSIC-QEDYEPDDEMGKLECGHSYHIHCIK 360

Query: 361 QWLAQKNTCPVCKTAAVGRG 374
           QWLAQKNTCPVCKTAAVGRG
Sbjct: 361 QWLAQKNTCPVCKTAAVGRG 375

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BLAST of MS021662 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 3.2e-28
Identity = 70/151 (46.36%), Postives = 89/151 (58.94%), Query Frame = 0


Query: 220 RSLEVSRSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELL 279
           R+  +S  R   H      +   E  M    L+  G  D HD+ RE+RLDVDNMSYEELL
Sbjct: 553 RNQIISEIRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELL 612

Query: 280 ELGERIGHVSTGLKEDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMG 339
            LGERIG VSTGL E+ I K +++ K    +  +S  L Q    C IC QE+Y   D +G
Sbjct: 613 ALGERIGDVSTGLSEEVILKAMKQHK-HTSSSPSSVELHQNIEPCCIC-QEEYVEGDNLG 672

Query: 340 KLECGHSYHIHCIKQWLAQKNTCPVCKTAAV 371
            L+CGH +H  CIKQW+  KN CP+CKT A+
Sbjct: 673 TLKCGHEFHKDCIKQWVMIKNLCPICKTEAL 701

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BLAST of MS021662 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 2.7e-27
Identity = 61/121 (50.41%), Postives = 84/121 (69.42%), Query Frame = 0


Query: 250 SLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKCIRKMKPFIV 309
           S+  GG  D HD+ R++RLD+DNMSYEELL L ERIG+VSTGL E+E+ K +++ K F  
Sbjct: 551 SIFYGG-VDIHDRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRK-FSS 610

Query: 310 NELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIKQWLAQKNTCPVCKTAA 369
             L +   S  +  C IC QE+Y   D++G L+CGH +H+ C++QWL  KNTCP+CK  A
Sbjct: 611 WRLEA---SVEEEPCCIC-QEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNTA 665

Query: 370 V 371
           +
Sbjct: 671 L 665

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BLAST of MS021662 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.7e-26
Identity = 63/128 (49.22%), Postives = 81/128 (63.28%), Query Frame = 0


Query: 243 EIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKCIR 302
           E  M    L+  G  + HD+ R++RLDVDNMSYEELL LGERIG VSTGL E+ I K ++
Sbjct: 542 EDYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKVMK 601

Query: 303 KMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIKQWLAQKNTC 362
           + K    +   SH   Q    C +C QE+Y   D++G L CGH +H  C+KQWL  KN C
Sbjct: 602 QHK-HTSSAAGSH---QDMEPCCVC-QEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLC 661

Query: 363 PVCKTAAV 371
           P+CKT A+
Sbjct: 662 PICKTVAL 664

[Close]

BLAST of MS021662 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.7e-26
Identity = 61/131 (46.56%), Postives = 84/131 (64.12%), Query Frame = 0


Query: 241 LAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKC 300
           L ++M+   S+L  G    HD++R++RLDVDNMSYEELL L ERIG V TG+ E+ I   
Sbjct: 560 LEDVMLLNHSVLFDGA-TGHDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEETISNR 619

Query: 301 IRKMKPFIVNELASHLLSQVDRK-CSICQQEDYEVDDEMGKLECGHSYHIHCIKQWLAQK 360
           +++ K        S+  S  D + C +C QE+Y   ++MG LECGH +H  CIK+WL QK
Sbjct: 620 LKQRK------YKSNTKSPQDAEPCCVC-QEEYTEGEDMGTLECGHEFHSQCIKEWLKQK 679

Query: 361 NTCPVCKTAAV 371
           N CP+CKT  +
Sbjct: 680 NLCPICKTTGL 682

[Close]

BLAST of MS021662 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.7e-21
Identity = 62/150 (41.33%), Postives = 88/150 (58.67%), Query Frame = 0


Query: 230 YRHVRHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVS 289
           +R++R   P+  A IM F     +    D H   R++RLD+D+M+YEELL L E+IG V+
Sbjct: 378 HRNIRILPPEH-ATIMDFSRLYEVSNVVDEH---RDMRLDIDSMTYEELLALEEQIGDVN 437

Query: 290 TGLKEDEIGKCIRKMKPFIVNELASHL------LSQVDRKCSICQQEDYEVDDEMGKLEC 349
           TGL +  I   + K+K  +    +S +       S  +  C IC QE+Y+V + +G L+C
Sbjct: 438 TGLAKSYI---VEKLKTSLFVPGSSCMSNKSSESSMENDACIIC-QEEYQVKECIGTLDC 497

Query: 350 GHSYHIHCIKQWLAQKNTCPVCKTAAVGRG 374
           GH YH  CIKQWL  KN CP+CKT A+  G
Sbjct: 498 GHRYHEDCIKQWLMVKNLCPICKTTALSTG 519

[Close]

BLAST of MS021662 vs. ExPASy TrEMBL
Match: A0A6J1C5U0 (uncharacterized protein LOC111008721 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008721 PE=4 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 8.0e-208
Identity = 370/373 (99.20%), Postives = 370/373 (99.20%), Query Frame = 0


Query: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS 60
           MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS
Sbjct: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS 60

Query: 61  GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK 120
           GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK
Sbjct: 61  GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK 120

Query: 121 SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG 180
           SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG
Sbjct: 121 SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG 180

Query: 181 RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG 240
           RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG
Sbjct: 181 RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG 240

Query: 241 LAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKC 300
           LAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKC
Sbjct: 241 LAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKC 300

Query: 301 IRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIKQWLAQKN 360
           IRKMKPFIVNELASHLLSQVDRKCSIC QEDYEVDDEMGKLECGHSYHIHCIKQWLAQKN
Sbjct: 301 IRKMKPFIVNELASHLLSQVDRKCSIC-QEDYEVDDEMGKLECGHSYHIHCIKQWLAQKN 360

Query: 361 TCPVCKTAAVGRG 374
            CPVCKTA VGRG
Sbjct: 361 MCPVCKTAVVGRG 372

[Close]

BLAST of MS021662 vs. ExPASy TrEMBL
Match: A0A6J1C605 (uncharacterized protein LOC111008721 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008721 PE=4 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 2.0e-206
Identity = 370/374 (98.93%), Postives = 370/374 (98.93%), Query Frame = 0


Query: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS 60
           MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS
Sbjct: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNS 60

Query: 61  GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK 120
           GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK
Sbjct: 61  GATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKKKQK 120

Query: 121 SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG 180
           SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG
Sbjct: 121 SSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRHASG 180

Query: 181 RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG 240
           RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG
Sbjct: 181 RGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPSPDG 240

Query: 241 LAE-IMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGK 300
           LAE IMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGK
Sbjct: 241 LAEQIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGK 300

Query: 301 CIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIKQWLAQK 360
           CIRKMKPFIVNELASHLLSQVDRKCSIC QEDYEVDDEMGKLECGHSYHIHCIKQWLAQK
Sbjct: 301 CIRKMKPFIVNELASHLLSQVDRKCSIC-QEDYEVDDEMGKLECGHSYHIHCIKQWLAQK 360

Query: 361 NTCPVCKTAAVGRG 374
           N CPVCKTA VGRG
Sbjct: 361 NMCPVCKTAVVGRG 373

[Close]

BLAST of MS021662 vs. ExPASy TrEMBL
Match: A0A0A0LQF5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042600 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.8e-182
Identity = 332/380 (87.37%), Postives = 353/380 (92.89%), Query Frame = 0


Query: 1   MPVVTQTTTVGEHIKLRRPR---GHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60
           MPVV    TVGEH+KLRRPR    HL+QPISESDPNPSIPSIIQSTRCKSTISSLLLSTF
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60

Query: 61  SN---SGATESLPSSSV-TNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKK 120
           SN   SG  ESLPSS + TNRKK+NFSSAT RGLGCT +ASQQVSVPAVIRTSADW+KKK
Sbjct: 61  SNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWEKKK 120

Query: 121 TRKKKQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVV 180
           TRKKKQKSSKNKTQQGI D ++F PNS+ NSASCLDAQDVWCGPGIGFS DAAASVDCVV
Sbjct: 121 TRKKKQKSSKNKTQQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 ARRHASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHV 240
           ARRHASGRGKID+EK++QRERSCLGRRTV+PETLLFLDSDS++PTARSLE+SRSRYYRHV
Sbjct: 181 ARRHASGRGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSEIPTARSLELSRSRYYRHV 240

Query: 241 RHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 300
           RHPSPDGLAEIMMFQSSLLMGGRFD HDQFRELRLDVDNMSYEELLELGERIGHVSTGLK
Sbjct: 241 RHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 300

Query: 301 EDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIK 360
           +DEIG+CIRKMKP +VNEL +HLLSQ+DRKCSIC QEDYE DDEMGKLECGHSYHIHCIK
Sbjct: 301 DDEIGRCIRKMKPHVVNELTTHLLSQMDRKCSIC-QEDYEPDDEMGKLECGHSYHIHCIK 360

Query: 361 QWLAQKNTCPVCKTAAVGRG 374
           QWLAQKNTCPVCKTAAVGRG
Sbjct: 361 QWLAQKNTCPVCKTAAVGRG 375

[Close]

BLAST of MS021662 vs. ExPASy TrEMBL
Match: A0A5D3BIX1 (E3 ubiquitin-protein ligase MBR2 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002180 PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.4e-180
Identity = 330/380 (86.84%), Postives = 350/380 (92.11%), Query Frame = 0


Query: 1   MPVVTQTTTVGEHIKLRRPR---GHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60
           MPVV    TVGEH+KLRRPR    HL+QPISESDPNPSIPSIIQSTRCKSTISSLLLSTF
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPSIPSIIQSTRCKSTISSLLLSTF 60

Query: 61  SN---SGATESLPSSSV-TNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKK 120
           SN   SG  ESLPSS + TNRKK+NFSSAT RGLGCT +ASQQVSVPAVIRTSADW+ KK
Sbjct: 61  SNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADWENKK 120

Query: 121 TRKKKQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVV 180
           TRKKKQKSSKNKTQQG+ D ++F PNS+ NSASCLDAQDVWCGPGIGFS DAAASVDCVV
Sbjct: 121 TRKKKQKSSKNKTQQGVLDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAASVDCVV 180

Query: 181 ARRHASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHV 240
           ARRHASGRGKID+EK++QRERSCLGRRTV+PETLLFLDSDSDL TARSLE+SR+RYYRHV
Sbjct: 181 ARRHASGRGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSDLSTARSLELSRTRYYRHV 240

Query: 241 RHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 300
           RHPSPDGLAEIMMFQSSLLMGGRFD HDQFRELRLDVDNMSYEELLELGERIGHVSTGLK
Sbjct: 241 RHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 300

Query: 301 EDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIK 360
           EDEIG+CIRKMKP +VNEL +HLLSQ+DRKCSIC QEDYE DDEMGKLECGHSYHI CIK
Sbjct: 301 EDEIGRCIRKMKPLVVNELTTHLLSQMDRKCSIC-QEDYEADDEMGKLECGHSYHIQCIK 360

Query: 361 QWLAQKNTCPVCKTAAVGRG 374
           QWLAQKNTCPVCKTAAVGRG
Sbjct: 361 QWLAQKNTCPVCKTAAVGRG 375

[Close]

BLAST of MS021662 vs. ExPASy TrEMBL
Match: A0A6J1GUE7 (E3 ubiquitin-protein ligase MBR2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457028 PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.4e-180
Identity = 325/376 (86.44%), Postives = 349/376 (92.82%), Query Frame = 0


Query: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSN- 60
           MPVVT+ +T+ EHIKLRRPR HL QPISESDPNPSIPSI+QSTRCKSTISSLLLSTF+N 
Sbjct: 1   MPVVTRASTMDEHIKLRRPRSHLRQPISESDPNPSIPSIVQSTRCKSTISSLLLSTFTNN 60

Query: 61  --SGATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTRKK 120
             +G  ESLPSS  TNRKK++FSSATFRGLGCT++ASQQV+VPAVIRTSADWQKK TRKK
Sbjct: 61  ATNGTNESLPSSVPTNRKKTHFSSATFRGLGCTSAASQQVTVPAVIRTSADWQKKNTRKK 120

Query: 121 KQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCVVARRH 180
           KQK+SKNKT QGI DG++F  NS FNSASCLDAQDVWCGPGIGFS DAAASVDCVVARRH
Sbjct: 121 KQKNSKNKTHQGIVDGSHFQHNSPFNSASCLDAQDVWCGPGIGFSADAAASVDCVVARRH 180

Query: 181 ASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRHVRHPS 240
           ASGRGKID+EKM+QRERS LGRRTVNPETLL L+SDSDLPTARSLEVSRSRYYRHVRHPS
Sbjct: 181 ASGRGKIDLEKMTQRERSYLGRRTVNPETLLLLESDSDLPTARSLEVSRSRYYRHVRHPS 240

Query: 241 PDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEI 300
           PDGLAEIMMFQSSLLMGGRFD  DQFRELRLDVD+MSYEELLELGERIGHVSTGLKEDEI
Sbjct: 241 PDGLAEIMMFQSSLLMGGRFDIRDQFRELRLDVDDMSYEELLELGERIGHVSTGLKEDEI 300

Query: 301 GKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIKQWLA 360
           G+CIRK KP +VNEL+S+LLSQ+DRKCSIC QEDYE DDEMG+L CGHSYHIHCIKQWLA
Sbjct: 301 GRCIRKTKPLVVNELSSYLLSQMDRKCSIC-QEDYEPDDEMGRLGCGHSYHIHCIKQWLA 360

Query: 361 QKNTCPVCKTAAVGRG 374
           QKNTCPVCKTAAVGRG
Sbjct: 361 QKNTCPVCKTAAVGRG 375

[Close]

BLAST of MS021662 vs. TAIR 10
Match: AT1G73760.1 (RING/U-box superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 7.4e-81
Identity = 179/386 (46.37%), Postives = 235/386 (60.88%), Query Frame = 0


Query: 2   PVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNSG 61
           P  + +TT+G+H++L+RPR H + P   +   P IP    S   KS +SS  L       
Sbjct: 7   PSSSSSTTIGQHMRLQRPRNHRNLPPISTADEPLIPK--PSRVSKSAMSSFFL------- 66

Query: 62  ATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKKKTR-KKKQK 121
               LP    T +KK N  +A+FRGLGCT SASQQVSVPAVIR+SADW     + KK +K
Sbjct: 67  ----LPE---TTKKKPN-GTASFRGLGCTTSASQQVSVPAVIRSSADWDASNFKIKKTKK 126

Query: 122 SSKNKTQQGIGDGT--YFHPNSNFNSASCLDAQDVWCGPGIGFSPDA--AASVDCVVA-- 181
            +KNK       G+       S  +S +C    DVWCGPG+GFS DA    S+D VV+  
Sbjct: 127 KNKNKGSSSYNGGSIKILSEASTSSSVACAAIPDVWCGPGVGFSTDAVVGGSIDTVVSDP 186

Query: 182 -RRHASGRGKIDVEKMSQRER-------SCLGRRTVNPETLLFLDSDSDLPTARSLEVSR 241
            RR+   R KID +K +           S L RR++N E+  + DSDS   T+R+ +   
Sbjct: 187 PRRNIPVRRKIDGDKTNSNSNNHREGSSSLLPRRSLNQESNPYFDSDSSFLTSRAEQT-- 246

Query: 242 SRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIG 301
            RY+RH+R P PDGLAE+MM Q+  +MGG   + DQFR++RL+VDNM+YE+LLELGERIG
Sbjct: 247 DRYHRHLRLPYPDGLAEMMMMQNGFVMGGVLSSFDQFRDMRLNVDNMTYEQLLELGERIG 306

Query: 302 HVSTGLKEDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHS 361
           HV+TGL E +I  C+RK+KP           +  DRKC ICQ E YE  DE+G+L CGH 
Sbjct: 307 HVNTGLTEKQIKSCLRKVKP------CRQDTTVADRKCIICQDE-YEAKDEVGELRCGHR 366

Query: 362 YHIHCIKQWLAQKNTCPVCKTAAVGR 373
           +HI C+ QWL +KN+CPVCKT A  +
Sbjct: 367 FHIDCVNQWLVRKNSCPVCKTMAYNK 366

[Close]

BLAST of MS021662 vs. TAIR 10
Match: AT1G17970.1 (RING/U-box superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 2.2e-69
Identity = 163/379 (43.01%), Postives = 227/379 (59.89%), Query Frame = 0


Query: 7   TTTVGEHIKLRRPRGHLSQPISESDPNPSIPSIIQSTRCKSTISSLLLSTFSNSGATESL 66
           +TT+GEHI+LRR R    + +  +D +P +  ++      +   +  +S+F        L
Sbjct: 3   STTIGEHIRLRRARNQTIRHLHAADDDPPLSHVVLPISQPNRFCNSAMSSFF------PL 62

Query: 67  P-SSSVTNRKKSNFSSATFRGLGC---TASASQQVSVPAVIRTSADWQKKKTRKKKQKSS 126
           P SSS  + +K  + +++FRG+GC    A+A+Q+VSVP+VIR SAD   +  + KK+K  
Sbjct: 63  PTSSSNESTRKKPYQTSSFRGMGCYAAAAAAAQEVSVPSVIRYSADLDARIRKDKKKKKH 122

Query: 127 KNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDA--AASVDCVVARRHASG 186
           K+K ++    G+Y   +    S    D  DVWC PG+GFS DA    SVD    R   S 
Sbjct: 123 KHKKKKKKNKGSYEDGSIRILSEEARDVIDVWCRPGLGFSTDAVIGRSVDPPRGRNIPSS 182

Query: 187 RGKIDVEKMSQRE---RSCLGRRTVNPETLL--FLDSDSDLPTARSLE--VSRSRYYRHV 246
           R KIDV+  +       S L  R +N ET      +SDS   T+   E  +  SR   H+
Sbjct: 183 RRKIDVDNNNYNHTLGSSVLPIRFLNQETHSHDIFNSDSTFVTSSRAEPTMLSSRCRGHL 242

Query: 247 RHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLK 306
               PD L E+ M Q+  +MG   D+ D + ELRLDVD+MSYE+LLELG+RIG+V+TGLK
Sbjct: 243 PRSYPDDLTEMRMLQNGFVMGRITDSRDNYHELRLDVDSMSYEQLLELGDRIGYVNTGLK 302

Query: 307 EDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIK 366
           E EI +C+ K+KP +     SH L  VDRKCSICQ E YE +DE+G+L CGHS+H+HC+K
Sbjct: 303 ESEIHRCLGKIKPSV-----SHTL--VDRKCSICQDE-YEREDEVGELNCGHSFHVHCVK 362

Query: 367 QWLAQKNTCPVCKTAAVGR 373
           QWL++KN CPVCK AA G+
Sbjct: 363 QWLSRKNACPVCKKAAYGK 367

[Close]

BLAST of MS021662 vs. TAIR 10
Match: AT1G45180.1 (RING/U-box superfamily protein )

HSP 1 Score: 132.9 bits (333), Expect = 5.4e-31
Identity = 63/139 (45.32%), Postives = 91/139 (65.47%), Query Frame = 0


Query: 232 HVRHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTG 291
           H+R      L ++M+   S+++G   D HD++R++RLDVDNM+YEELL L ERIG V TG
Sbjct: 506 HMRRAGNLRLEDVMLLNQSIMLGAA-DIHDRYRDMRLDVDNMTYEELLSLEERIGDVCTG 565

Query: 292 LKEDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHC 351
           L E+ I   +++ K       +S   SQ    C +C QE+Y+ ++E+G+LECGH +H  C
Sbjct: 566 LNEETISNRLKQQK-----YKSSTRSSQEVEPCCVC-QEEYKEEEEIGRLECGHDFHSQC 625

Query: 352 IKQWLAQKNTCPVCKTAAV 371
           IK+WL QKN CP+CKT  +
Sbjct: 626 IKEWLKQKNLCPICKTTGL 637

[Close]

BLAST of MS021662 vs. TAIR 10
Match: AT4G31450.1 (RING/U-box superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-30
Identity = 63/132 (47.73%), Postives = 91/132 (68.94%), Query Frame = 0


Query: 243 EIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGKCIR 302
           ++++ ++ LL+GG    HDQ R++RLD+DNMSYEELL L ERIG VST L E+ I KC++
Sbjct: 363 DLLIMETGLLLGG-LSFHDQHRDMRLDIDNMSYEELLALEERIGTVSTALTEEAISKCLK 422

Query: 303 ----KMKPFIVNELA-SHLLSQVDRKCSICQQEDYEVDDEMGKLECGHSYHIHCIKQWLA 362
               +MKP     +  S   ++ D KCSIC QE+Y + DE+G+L C H+YH+ C+++WL 
Sbjct: 423 TSIYQMKPLSYGSITKSPSDNKEDAKCSIC-QEEYTIGDEVGRLHCEHTYHVKCVQEWLR 482

Query: 363 QKNTCPVCKTAA 370
            K+ CP+CK  A
Sbjct: 483 IKSWCPICKATA 492

[Close]

BLAST of MS021662 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 2.3e-29
Identity = 70/151 (46.36%), Postives = 89/151 (58.94%), Query Frame = 0


Query: 220 RSLEVSRSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELL 279
           R+  +S  R   H      +   E  M    L+  G  D HD+ RE+RLDVDNMSYEELL
Sbjct: 553 RNQIISEIRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELL 612

Query: 280 ELGERIGHVSTGLKEDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQQEDYEVDDEMG 339
            LGERIG VSTGL E+ I K +++ K    +  +S  L Q    C IC QE+Y   D +G
Sbjct: 613 ALGERIGDVSTGLSEEVILKAMKQHK-HTSSSPSSVELHQNIEPCCIC-QEEYVEGDNLG 672

Query: 340 KLECGHSYHIHCIKQWLAQKNTCPVCKTAAV 371
            L+CGH +H  CIKQW+  KN CP+CKT A+
Sbjct: 673 TLKCGHEFHKDCIKQWVMIKNLCPICKTEAL 701

The following BLAST results are available for this feature:

BLAST of MS021662 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137196.1	1.7e-207	99.20	uncharacterized protein LOC111008721 isoform X2 [Momordica charantia]	[more]
XP_022137195.1	4.1e-206	98.93	uncharacterized protein LOC111008721 isoform X1 [Momordica charantia]	[more]
XP_038894454.1	1.2e-184	87.89	E3 ubiquitin-protein ligase MBR2 isoform X2 [Benincasa hispida]	[more]
XP_038894453.1	2.8e-183	87.66	E3 ubiquitin-protein ligase MBR2 isoform X1 [Benincasa hispida]	[more]
XP_004137488.1	1.2e-181	87.37	E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis sativus]	[more]

BLAST of MS021662 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQF9	3.2e-28	46.36	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]
Q7XTV7	2.7e-27	50.41	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]
O49500	1.7e-26	49.22	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV...	[more]
Q9FMM4	1.7e-26	46.56	Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q5QLR5	1.7e-21	41.33	Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of MS021662 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C5U0	8.0e-208	99.20	uncharacterized protein LOC111008721 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1C605	2.0e-206	98.93	uncharacterized protein LOC111008721 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A0A0LQF5	5.8e-182	87.37	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042600 P...	[more]
A0A5D3BIX1	2.4e-180	86.84	E3 ubiquitin-protein ligase MBR2 isoform X2 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A6J1GUE7	2.4e-180	86.44	E3 ubiquitin-protein ligase MBR2-like isoform X2 OS=Cucurbita moschata OX=3662 G...	[more]

BLAST of MS021662 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73760.1	7.4e-81	46.37	RING/U-box superfamily protein	[more]
AT1G17970.1	2.2e-69	43.01	RING/U-box superfamily protein	[more]
AT1G45180.1	5.4e-31	45.32	RING/U-box superfamily protein	[more]
AT4G31450.1	1.2e-30	47.73	RING/U-box superfamily protein	[more]
AT2G15530.1	2.3e-29	46.36	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 324..365 e-value: 3.3E-7 score: 39.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 323..366 e-value: 1.4E-12 score: 47.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 324..366 score: 12.164712
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 302..370 e-value: 2.5E-16 score: 60.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 107..135
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..36
None	No IPR available	PANTHER	PTHR22937	E3 UBIQUITIN-PROTEIN LIGASE RNF165	coord: 6..368
None	No IPR available	PANTHER	PTHR22937:SF125	C3HC4-TYPE RING ZINC FINGER PROTEIN	coord: 6..368
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 315..368

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021662.1	MS021662.1	mRNA