MS021656 (gene) Bitter gourd (TR) v1

BLAST of MS021656 vs. NCBI nr
Match: XP_022137292.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111008789 [Momordica charantia])

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 593/638 (92.95%), Postives = 604/638 (94.67%), Query Frame = 0

Query: 249 SASSSSANVFFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRRE 308
           S S++ +NVFFIAFHLSLCFTVDAIDEFKACGHSYNCGELV+IAYPFWGNERKGFCGRRE
Sbjct: 4   SQSTTISNVFFIAFHLSLCFTVDAIDEFKACGHSYNCGELVNIAYPFWGNERKGFCGRRE 63

Query: 309 FELKCKHNRTTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFV 368
           FELKCKHNRTTTIQINSLEYN+LKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFV
Sbjct: 64  FELKCKHNRTTTIQINSLEYNILKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFV 123

Query: 369 YSSNDQNISVRYNCSAQKEIPETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEECTMNI 428
           YSSNDQNISVRYNCSAQKEIPETYKFSCG EEEKNGRANYAFEPSAATWNLLIEECTMNI
Sbjct: 124 YSSNDQNISVRYNCSAQKEIPETYKFSCGSEEEKNGRANYAFEPSAATWNLLIEECTMNI 183

Query: 429 DVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYC 488
           DVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYC
Sbjct: 184 DVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYC 243

Query: 489 ICRSGEIHPYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKMNAMAGVGCGIGGIILM 548
           ICRSGEIHPYFCGTAHAL   + ++                   N + GVGCGIGGIILM
Sbjct: 244 ICRSGEIHPYFCGTAHALEESNDKW------------------KNTVIGVGCGIGGIILM 303

Query: 549 GTISLILLRLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMGVPLFSYGELEEATDKFN 608
           GTISLILLRLQKNKHAHASSFPLGNNYFDPPSKELEKGENYM VPLFSYGELEEATDKFN
Sbjct: 304 GTISLILLRLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMVVPLFSYGELEEATDKFN 363

Query: 609 PAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYG 668
           PAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYG
Sbjct: 364 PAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYG 423

Query: 669 CTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIETASALAYLHASKTI 728
           CTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPW TRLKIAIETASALAYLHASKTI
Sbjct: 424 CTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPWITRLKIAIETASALAYLHASKTI 483

Query: 729 HRDVKTTNILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDPEYQECYQLTNKS 788
           HRDVKTTNILLDKN  VKVADLGLSCLSPTQATHVSTAPQGTPGY+DPEYQECYQLTNKS
Sbjct: 484 HRDVKTTNILLDKNLSVKVADLGLSCLSPTQATHVSTAPQGTPGYIDPEYQECYQLTNKS 543

Query: 789 DVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVR 848
           DVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVR
Sbjct: 544 DVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVR 603

Query: 849 DMIYAAAELAFRCLQSVKDMRPSMLEALEILKNIESRS 887
           DMIYA A LAF+CLQSVKDMRPSMLEALEILKNIESRS
Sbjct: 604 DMIYAVAGLAFQCLQSVKDMRPSMLEALEILKNIESRS 623

BLAST of MS021656 vs. NCBI nr
Match: XP_022137293.1 (uncharacterized protein LOC111008790 [Momordica charantia])

HSP 1 Score: 914.4 bits (2362), Expect = 7.3e-262
Identity = 507/906 (55.96%), Postives = 593/906 (65.45%), Query Frame = 0

Query: 1    KNKLGQGGFSTVYKGKLPDGRDVAVKLLNESKENGQDFINEVVSITKTSHVNIATLLGFC 60
            KNKLGQGGFSTVYKGKLPDGRDVAVKLLNESKENGQDFINEVVSIT TSHVNIATLLGFC
Sbjct: 335  KNKLGQGGFSTVYKGKLPDGRDVAVKLLNESKENGQDFINEVVSITTTSHVNIATLLGFC 394

Query: 61   YERNRRALVYEYMPKGSLDKYIFHKDPQKSDMGLDWNTLYTIVIGVARGLEYLHRGCNTR 120
            YERNRRALVYEYMPKGSLDKYIFHKDPQKSDMGLDWNTLYTIVIGVARGLEYLHRGCNTR
Sbjct: 395  YERNRRALVYEYMPKGSLDKYIFHKDPQKSDMGLDWNTLYTIVIGVARGLEYLHRGCNTR 454

Query: 121  ILHFDIKPHNILLDNEFCPKISDFGLAKQCRARESHVSMTGVKGTIGFMAPEVVFRNIGK 180
            ILHFDIKPHNILLDNEFCPKISDFGLAKQCRARESHVSMTGVKGTIGFMAPEVVFRNIGK
Sbjct: 455  ILHFDIKPHNILLDNEFCPKISDFGLAKQCRARESHVSMTGVKGTIGFMAPEVVFRNIGK 514

Query: 181  VSHKSDVYSYGMLVLEMVGERKNPNEGVGQRSEEYFPDWIYKDLTQSETDEG--W----- 240
            VSHKSDVYSYGMLVLEMVGERKNPNEGVGQRSEEYFPDWIYKDLTQSETDEG  W     
Sbjct: 515  VSHKSDVYSYGMLVLEMVGERKNPNEGVGQRSEEYFPDWIYKDLTQSETDEGCSWGNTEE 574

Query: 241  -----------------------------------NADALQIPPKPNLFGS--------- 300
                                               + D LQIPPKPN  G+         
Sbjct: 575  EDEMARKMIIVGLHCIQTLPDDRPSMSDVVGMLEGSVDGLQIPPKPNFMGTPTTTSTTLP 634

Query: 301  --ASSSSAN-----------------------------------VFFIAFHLSLCFTVDA 360
              +SSS +N                                   + FIAFHLS  F    
Sbjct: 635  HPSSSSPSNFLMNPVVQKLNKMRIIHLSLTSFGLNHTFIGNFLFISFIAFHLSPSF---G 694

Query: 361  IDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRREFELKCKHNRTTTIQINSLEYNVLK 420
             DEFKACG SYNCGELV+I YPFWGN+R+GFCGRREFEL CK NRTTTIQINS +YNVL+
Sbjct: 695  DDEFKACGISYNCGELVNITYPFWGNKRQGFCGRREFELNCKRNRTTTIQINSFDYNVLR 754

Query: 421  INQSDHRMTIARSDLF--DNLCPKNQTQTATLDHHLFVYSSNDQNISVRYNCSAQKEIPE 480
            INQ+DHRMTIARSDL    NLCPKN+T TA LD+++F YS ND  IS+ Y+C  Q E  E
Sbjct: 755  INQTDHRMTIARSDLLVPGNLCPKNKTGTANLDYNVFRYSENDLQISLWYDCPLQGENVE 814

Query: 481  TYKFSCGLEEEKNGRANYAFEPSAATWNLLIE-ECTMNIDVKVTMEGLKEGL-KNRTRLV 540
            +Y+FSCG E EKNG  NYAF+     W    E +C  N++VKV  EG + G  KNRT  V
Sbjct: 815  SYRFSCGGEGEKNGSGNYAFDAMQTIWRNQFEGKCRTNVEVKVRREGWEGGNGKNRTGAV 874

Query: 541  EKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYCICRSGEIHPYFCGTAHALGT 600
            E A+R GFDVEYGNLY+  C  C+ +GG CGGNGTYPFYCIC S +  P  C  A A G 
Sbjct: 875  ESALRGGFDVEYGNLYSTECEGCKESGGKCGGNGTYPFYCICESQDAQPSACRAAAAPGV 934

Query: 601  FSPRFHITYIIITFGNSTNLTRKMNAMAGVGCGIGGIILMGTISLILLRLQKNKHAHASS 660
             S RF     II                 V   +GGII + ++ +I++   +N       
Sbjct: 935  -SYRFPKKISII-----------------VAIVLGGIISIISLVVIIIYHTRNSIPKDKI 994

Query: 661  FPLGNNYFDPPSKELEKGENYMGVPLFSYGELEEATDKFNPAKELGDGGFGTVYYGKLRD 720
              + + Y     K             +SY +L++ TD FN   +LG GGF TVY GKL D
Sbjct: 995  DEIISRYSTHTPKR------------YSYSKLKKITDSFN--NKLGQGGFSTVYRGKLPD 1054

Query: 721  GREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYG-CTSRRSRELLLVYEFIPNG 780
            GR+VAVK L E+  +  + FMNEV  +T   H ++V++ G C  R  R   L+YE++P G
Sbjct: 1055 GRDVAVKLLNESRDQNGQDFMNEVVSITRTSHVNIVSILGFCYERNKR--ALIYEYMPKG 1114

Query: 781  TLAD---HLHGNRAKSGELPWNTRLKIAIETASALAYLHA---SKTIHRDVKTTNILLDK 805
            +L     H    R+   +L WNT   IA+  A  L YLH    ++ +H D+K  NILLD 
Sbjct: 1115 SLDKYIFHKDPQRSNVEKLDWNTVYSIAVGVARGLEYLHQGCNTRILHFDIKPHNILLDN 1174

BLAST of MS021656 vs. NCBI nr
Match: XP_023519142.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 857.4 bits (2214), Expect = 1.1e-244
Identity = 436/630 (69.21%), Postives = 495/630 (78.57%), Query Frame = 0

Query: 258 FFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRREFELKCKHNR 317
           FF  FHLS CFT +A+D FK CG +YNCGELV+I YPFWGN+R+  CGR+EF+L C +NR
Sbjct: 9   FFFTFHLSPCFTDNALDAFKDCGVNYNCGELVNITYPFWGNKRQSSCGRKEFKLNCNNNR 68

Query: 318 TTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFVYSSNDQNIS 377
           TTT  INSLEYNVL+INQ ++ M IARSDLFDN CP+NQ Q A++DHH F YS+ +QNIS
Sbjct: 69  TTTTYINSLEYNVLEINQLNNTMRIARSDLFDNYCPRNQIQVASMDHHPFAYSARNQNIS 128

Query: 378 VRYNCSAQKEIP-ETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEECTMNIDVKVTMEG 437
           V YNCS   E P   Y FSCG E E+ GR NY FEPSAA ++ L+ EC M I+V VT++G
Sbjct: 129 VGYNCSPDNEFPVSEYNFSCGGEWERRGRVNYGFEPSAAVYSKLV-ECEMKIEVMVTIKG 188

Query: 438 LKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYCICRSGEIH 497
           LKEG+KNR  LVE AV+ GF+VEY N Y  AC+ C  +GGNCGGNGTYPFYCICR+G  +
Sbjct: 189 LKEGVKNRRSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRNGVAN 248

Query: 498 PYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKMNAMAGVGCGIGGIILMGTISLILL 557
           PY C    A     PR            + N T K   + G G GIGGI++M  I  I L
Sbjct: 249 PYACDATAA----PPR-------PPHPENPNDTWK-KVVIGAGSGIGGIVMMSIIFFIRL 308

Query: 558 RLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMGVPLFSYGELEEATDKFNPAKELGDG 617
           R+ K KH + S         D P KELEK E+YM + LFSY EL +ATDKFNPA ELGDG
Sbjct: 309 RMHKKKHQNYSC--------DRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGDG 368

Query: 618 GFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYGCTSRRSRE 677
           GFGTVYYGKLRDGREVAVKRLFENNYRKVE FMNEV+ILT L H HLVTLYGCTSRRSRE
Sbjct: 369 GFGTVYYGKLRDGREVAVKRLFENNYRKVEQFMNEVEILTRLRHPHLVTLYGCTSRRSRE 428

Query: 678 LLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIETASALAYLHASKTIHRDVKTTN 737
           LLLVYEFIPNGT+ADHLHG RAK GELPW+TRLKIAIETASALA+LHAS+TIHRDVKTTN
Sbjct: 429 LLLVYEFIPNGTVADHLHGERAKPGELPWHTRLKIAIETASALAFLHASETIHRDVKTTN 488

Query: 738 ILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDPEYQECYQLTNKSDVFSFGVV 797
           ILLD NF VKVAD GL  L PTQA+HVSTAPQGTPGY+DPEY E YQ+TNKSDVFSFGVV
Sbjct: 489 ILLDNNFGVKVADFGLCRLFPTQASHVSTAPQGTPGYIDPEYYESYQVTNKSDVFSFGVV 548

Query: 798 LVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVRDMIYAAAE 857
           LVELISSKPAVD+TRHRHEINL TMAINKIQN++LH+FVDPSL F+ DQ +RDMI   AE
Sbjct: 549 LVELISSKPAVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKIDQRIRDMICGVAE 608

Query: 858 LAFRCLQSVKDMRPSMLEALEILKNIESRS 887
           LAF+CLQSV+D RPSM+EALEIL+NIE+RS
Sbjct: 609 LAFQCLQSVRDTRPSMMEALEILRNIENRS 617

BLAST of MS021656 vs. NCBI nr
Match: XP_023000837.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 857.1 bits (2213), Expect = 1.4e-244
Identity = 438/630 (69.52%), Postives = 498/630 (79.05%), Query Frame = 0

Query: 258 FFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRREFELKCKHNR 317
           F   FHLS CF+ +A+D FK CG SYNCGELV+I YPFWGNE++  CGR+EF+L CK NR
Sbjct: 8   FLFTFHLSTCFSDNALDAFKDCGVSYNCGELVNITYPFWGNEKQSSCGRKEFKLNCKSNR 67

Query: 318 TTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFVYSSNDQNIS 377
           TTT  INSLEYNVL+INQS++ M IARSDLFDN CP+NQ Q A++DHH F YSS +QNIS
Sbjct: 68  TTTTYINSLEYNVLEINQSNNTMRIARSDLFDNYCPRNQIQVASIDHHPFAYSSRNQNIS 127

Query: 378 VRYNCSAQKE-IPETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEECTMNIDVKVTMEG 437
           V YNCS   E +   YKFSCG E ++ GR NYAFEPSAA ++ L  EC M I+V VT++ 
Sbjct: 128 VGYNCSPDNEFLISKYKFSCGGEWQRRGRVNYAFEPSAAVYSKL-AECEMKIEVMVTIKE 187

Query: 438 LKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYCICRSGEIH 497
           LKEGLKNRT LVE AV+ GF+VEY N Y  AC+ C  +GGNCGGNGTYPFYCICR+   +
Sbjct: 188 LKEGLKNRTSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRNEVAN 247

Query: 498 PYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKMNAMAGVGCGIGGIILMGTISLILL 557
           PY C    A     PR            + N T K   + G G GIGGI++M  I  I L
Sbjct: 248 PYACDAIAA----PPR-------PPHPENPNDTWK-KVVIGAGSGIGGIVMMSIIFFIRL 307

Query: 558 RLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMGVPLFSYGELEEATDKFNPAKELGDG 617
           R+ K KH + S         D P KELEK E+YM + LFSY EL +ATDKFNPA ELGDG
Sbjct: 308 RMHKKKHQNYSC--------DRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGDG 367

Query: 618 GFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYGCTSRRSRE 677
           GFGTVYYGKLRDGREVAVKRLFENNYRKVE FMNEV++LT L H HLVTLYGCTSRRSRE
Sbjct: 368 GFGTVYYGKLRDGREVAVKRLFENNYRKVEQFMNEVEMLTRLRHPHLVTLYGCTSRRSRE 427

Query: 678 LLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIETASALAYLHASKTIHRDVKTTN 737
           LLLVY+FIPNGT+ADHLHGNRA+SGELPW TRLK+AIETASALA+LHAS+TIHRDVKTTN
Sbjct: 428 LLLVYKFIPNGTVADHLHGNRARSGELPWLTRLKVAIETASALAFLHASETIHRDVKTTN 487

Query: 738 ILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDPEYQECYQLTNKSDVFSFGVV 797
           ILLD NF VKVAD GLS L PTQA+HVSTAPQGTPGY+DPEY E YQLTNKSDVFSFGVV
Sbjct: 488 ILLDNNFGVKVADFGLSRLFPTQASHVSTAPQGTPGYIDPEYYESYQLTNKSDVFSFGVV 547

Query: 798 LVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVRDMIYAAAE 857
           LVELISSKPAVD+TRHRHEINL TMAINKIQN++LH+FVDPSL F+TDQ +R+MI   AE
Sbjct: 548 LVELISSKPAVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKTDQRIRNMICGVAE 607

Query: 858 LAFRCLQSVKDMRPSMLEALEILKNIESRS 887
           LAF+CLQSV+D RPSM+EALEIL+NIE+RS
Sbjct: 608 LAFQCLQSVRDTRPSMMEALEILRNIENRS 616

BLAST of MS021656 vs. NCBI nr
Match: XP_023000835.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 856.3 bits (2211), Expect = 2.4e-244
Identity = 439/631 (69.57%), Postives = 501/631 (79.40%), Query Frame = 0

Query: 258 FFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRREFELKCKHNR 317
           F   FHLS CF+ +A+D FK CG SYNCGELV+I YPFWGNE++  CGR+EF+L CK NR
Sbjct: 8   FLFTFHLSTCFSDNALDAFKDCGVSYNCGELVNITYPFWGNEKQSSCGRKEFKLNCKSNR 67

Query: 318 TTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFVYSSNDQNIS 377
           TTT  INSLEYNVL+INQS++ M IARSDLFDN CP+NQ Q A++DHH F YSS +QNIS
Sbjct: 68  TTTTYINSLEYNVLEINQSNNTMRIARSDLFDNYCPRNQIQVASIDHHPFAYSSRNQNIS 127

Query: 378 VRYNCSAQKE-IPETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEECTMNIDVKVTMEG 437
           V YNCS   E +   YKFSCG E ++ GR NYAFEPSAA ++ L  EC M I+V VT++ 
Sbjct: 128 VGYNCSPDNEFLISKYKFSCGGEWQRRGRVNYAFEPSAAVYSKL-AECEMKIEVMVTIKE 187

Query: 438 LKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYCICRSGEIH 497
           LKEGLKNRT LVE AV+ GF+VEY N Y  AC+ C  +GGNCGGNGTYPFYCICR+   +
Sbjct: 188 LKEGLKNRTSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRNEVAN 247

Query: 498 PYFCGTAHALGTFSPR-FHITYIIITFGNSTNLTRKMNAMAGVGCGIGGIILMGTISLIL 557
           PY C    A     PR  H ++       + N T K   + G G GIGGI++M  I  I 
Sbjct: 248 PYACDAIAA----PPRPPHPSH-----PENPNDTWK-KVVIGAGSGIGGIVMMSIIFFIR 307

Query: 558 LRLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMGVPLFSYGELEEATDKFNPAKELGD 617
           LR+ K KH + S         D P KELEK E+YM + LFSY EL +ATDKFNPA ELGD
Sbjct: 308 LRMHKKKHQNYSC--------DRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGD 367

Query: 618 GGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYGCTSRRSR 677
           GGFGTVYYGKLRDGREVAVKRLFENNYRKVE FMNEV++LT L H HLVTLYGCTSRRSR
Sbjct: 368 GGFGTVYYGKLRDGREVAVKRLFENNYRKVEQFMNEVEMLTRLRHPHLVTLYGCTSRRSR 427

Query: 678 ELLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIETASALAYLHASKTIHRDVKTT 737
           ELLLVY+FIPNGT+ADHLHGNRA+SGELPW TRLK+AIETASALA+LHAS+TIHRDVKTT
Sbjct: 428 ELLLVYKFIPNGTVADHLHGNRARSGELPWLTRLKVAIETASALAFLHASETIHRDVKTT 487

Query: 738 NILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDPEYQECYQLTNKSDVFSFGV 797
           NILLD NF VKVAD GLS L PTQA+HVSTAPQGTPGY+DPEY E YQLTNKSDVFSFGV
Sbjct: 488 NILLDNNFGVKVADFGLSRLFPTQASHVSTAPQGTPGYIDPEYYESYQLTNKSDVFSFGV 547

Query: 798 VLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVRDMIYAAA 857
           VLVELISSKPAVD+TRHRHEINL TMAINKIQN++LH+FVDPSL F+TDQ +R+MI   A
Sbjct: 548 VLVELISSKPAVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKTDQRIRNMICGVA 607

Query: 858 ELAFRCLQSVKDMRPSMLEALEILKNIESRS 887
           ELAF+CLQSV+D RPSM+EALEIL+NIE+RS
Sbjct: 608 ELAFQCLQSVRDTRPSMMEALEILRNIENRS 619

BLAST of MS021656 vs. ExPASy Swiss-Prot
Match: F4HQ17 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.4 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.4 PE=2 SV=2)

HSP 1 Score: 468.0 bits (1203), Expect = 2.4e-130
Identity = 281/656 (42.84%), Postives = 384/656 (58.54%), Query Frame = 0

Query: 253 SSANVFFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRREFELK 312
           SS+ +FFI F  SL + +    E   C   + CG  ++ ++PFWG +R   CG    EL+
Sbjct: 6   SSSLMFFILF--SLFYHLPC--ESSKCESLFQCGN-ITASFPFWGGDRHKHCGHPLLELR 65

Query: 313 CKHNRTTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFVYSSN 372
           C  N++T++ I+  E+ VL ++Q+ + +T+AR DL  + C    T T TL   +F  S  
Sbjct: 66  CDQNKSTSLFISDQEFFVLHVDQTSYSLTLARPDLLHSFCSLTFTNT-TLPPEIFELSPA 125

Query: 373 DQNISVRYNCSAQKEIPETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEECTMNIDVKV 432
            ++++  Y+C     +P+   ++C +     G  + +  P         E C  N    V
Sbjct: 126 YKSVTF-YHC--YPVLPDLSNYTCPV----IGPISVSGNPEDH------ETCFPNFAANV 185

Query: 433 TMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYCICRS 492
               + +  K     +E  + +GF+V   N+   AC  C  +  +CG +  +PF   C+ 
Sbjct: 186 PTSFVTKEKKLNIANLESVLEKGFEVNM-NVIMKACQACSYSNESCGFDENFPFEVKCK- 245

Query: 493 GEIHPYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKMNAMAGVG-CGIGG--IILMG 552
               P+                         + T+ +  + A AG+    + G  I+L+ 
Sbjct: 246 ----PHH------------------------SPTDTSLSIGAKAGIAVASVSGLAILLLA 305

Query: 553 TISLILLRLQKNKHAH--ASSFPL--------------------GNNYFDPPSKELEKGE 612
            + L + R +K + A   + S P+                     N+   P    L    
Sbjct: 306 GLFLCIRRRRKTQDAQYTSKSLPITSYSSRDTSRNPTSTTISSSSNHSLLPSISNLANRS 365

Query: 613 NYMGVPLFSYGELEEATDKFNPAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEH 672
           +Y GV +FSY ELEEAT+ F  ++ELGDGGFGTVYYG L+DGR VAVKRL+E + ++VE 
Sbjct: 366 DYCGVQVFSYEELEEATENF--SRELGDGGFGTVYYGVLKDGRAVAVKRLYERSLKRVEQ 425

Query: 673 FMNEVKILTCLHHQHLVTLYGCTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPWNT 732
           F NE++IL  L H +LV LYGCTSR SRELLLVYE+I NGTLA+HLHGNRA++  L W+T
Sbjct: 426 FKNEIEILKSLKHPNLVILYGCTSRHSRELLLVYEYISNGTLAEHLHGNRAEARPLCWST 485

Query: 733 RLKIAIETASALAYLHASKTIHRDVKTTNILLDKNFCVKVADLGLSCLSPTQATHVSTAP 792
           RL IAIETASAL++LH    IHRD+KTTNILLD N+ VKVAD GLS L P   TH+STAP
Sbjct: 486 RLNIAIETASALSFLHIKGIIHRDIKTTNILLDDNYQVKVADFGLSRLFPMDQTHISTAP 545

Query: 793 QGTPGYVDPEYQECYQLTNKSDVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQ 852
           QGTPGYVDPEY +CYQL  KSDV+SFGVVL ELISSK AVD+TRHRH+INL  MA++KIQ
Sbjct: 546 QGTPGYVDPEYYQCYQLNEKSDVYSFGVVLTELISSKEAVDITRHRHDINLANMAVSKIQ 605

Query: 853 NNDLHNFVDPSLRFETDQSVRDMIYAAAELAFRCLQSVKDMRPSMLEALEILKNIE 884
           NN LH  VD SL ++ D  VR  + A AELAFRCLQ  +D+RP+M E +EIL+ I+
Sbjct: 606 NNALHELVDSSLGYDNDPEVRRKMMAVAELAFRCLQQERDVRPAMDEIVEILRGIK 610

BLAST of MS021656 vs. ExPASy Swiss-Prot
Match: P0C5E2 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.2 PE=2 SV=3)

HSP 1 Score: 457.6 bits (1176), Expect = 3.2e-127
Identity = 279/644 (43.32%), Postives = 375/644 (58.23%), Query Frame = 0

Query: 259 FIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFW-GNERKGFCGRREFELKC-KHN 318
           F  F +    T+    +FKAC    +CG+   I+YPF+   +++ FCG   FEL C    
Sbjct: 14  FFYFTIIATQTLSLDPKFKAC-EPKSCGKGPQISYPFYLSGKQESFCGYPSFELTCDDEE 73

Query: 319 RTTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFVYSSNDQNI 378
           +   + I+  EY +  I+       +  S    + CP+      TL    F  + +  N 
Sbjct: 74  KLPVLGISGEEYVIKNISYLTQSFQVVNSKASHDPCPR-PLNNLTLHRTPFFVNPSHINF 133

Query: 379 SVRYNCSAQ-KEIPETYKFSCG-----------LEEEKNGRANYAFEPSAATWNLLIEEC 438
           ++ YNCS    E   TY  +C             + +K G+     E   A+ +     C
Sbjct: 134 TILYNCSDHLLEDFRTYPLTCARNTSLLRSFGVFDRKKLGK-----EKQIASMS-----C 193

Query: 439 TMNIDVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTY 498
              +DV V +   +  +   T +  + ++RGF +   N    +C  C  +GG C G    
Sbjct: 194 QKLVDVPV-LASNESDVMGMTYV--EILKRGFVL---NWTANSCFRCITSGGRC-GTDQQ 253

Query: 499 PFYCICRSGEIHPYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRK-MNAMAGVGCGIG 558
            F C+C  G                 P+ H T    T G +    R  +  + G    + 
Sbjct: 254 EFVCLCPDG-----------------PKLHDT---CTNGKNDKRRRVIVKVLIGASAAVV 313

Query: 559 GIILMGTISLILLRLQKNKHAHASSFPLGNNYFDPPSK--ELEKGENYM-GVPLFSYGEL 618
           G+I       +  R +   + ++S+    N   DP +K  ++EK E  + GV +FSY EL
Sbjct: 314 GLIAASIFWYVYHRRKTKSYRNSSALLPRNISSDPSAKSFDIEKAEELLVGVHIFSYEEL 373

Query: 619 EEATDKFNPAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHH 678
           EEAT+ F+P+KELGDGGFGTVYYGKL+DGR VAVKRL++NN+++ E F NEV+ILT L H
Sbjct: 374 EEATNNFDPSKELGDGGFGTVYYGKLKDGRSVAVKRLYDNNFKRAEQFRNEVEILTGLRH 433

Query: 679 QHLVTLYGCTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIETASALA 738
            +LV L+GC+S++SR+LLLVYE++ NGTLADHLHG +A    LPW+ RLKIA+ETASAL 
Sbjct: 434 PNLVALFGCSSKQSRDLLLVYEYVANGTLADHLHGPQANPSSLPWSIRLKIAVETASALK 493

Query: 739 YLHASKTIHRDVKTTNILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDPEYQE 798
           YLHASK IHRDVK+ NILLD+NF VKVAD GLS L P   THVSTAPQGTPGYVDP+Y  
Sbjct: 494 YLHASKIIHRDVKSNNILLDQNFNVKVADFGLSRLFPMDKTHVSTAPQGTPGYVDPDYHL 553

Query: 799 CYQLTNKSDVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLR 858
           CYQL+NKSDV+SF VVL+ELISS PAVD+TR R EINL  MA+ KIQN++L + VDPSL 
Sbjct: 554 CYQLSNKSDVYSFAVVLMELISSLPAVDITRPRQEINLSNMAVVKIQNHELRDMVDPSLG 613

Query: 859 FETDQSVRDMIYAAAELAFRCLQSVKDMRPSMLEALEILKNIES 885
           F+TD  VR  + A AELAF+CLQS KD+RP M    + L  I++
Sbjct: 614 FDTDTRVRQTVIAVAELAFQCLQSDKDLRPCMSHVQDTLTRIQN 618

BLAST of MS021656 vs. ExPASy Swiss-Prot
Match: Q8VYG0 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.3 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.3 PE=2 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 1.5e-124
Identity = 283/666 (42.49%), Postives = 378/666 (56.76%), Query Frame = 0

Query: 247 FGSASSSSANVFFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGR 306
           F   +S    +FF+++   + F   A  + + C   + CG+L +  +PFWG  R   CG 
Sbjct: 9   FSKPNSFLVLLFFLSY---IHFLPCAQSQREPCDTLFRCGDLTA-GFPFWGVARPQPCGH 68

Query: 307 REFELKC-KHNRTTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHH 366
               L C K   +T++ I+SL Y VL++N +   + + R D     C  +    ATL   
Sbjct: 69  PSLGLHCQKQTNSTSLIISSLMYRVLEVNTTTSTLKLVRQDFSGPFCSAS-FSGATLTPE 128

Query: 367 LFVYSSNDQNISVRYNCSAQKEIPETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEEC- 426
           LF    + + +S  Y C+     P   KF C       G  +   +      +L    C 
Sbjct: 129 LFELLPDYKTLSAYYLCNPSLHYPA--KFIC----PNKGVGSIHQD------DLYHNHCG 188

Query: 427 -TMNIDVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGT 486
              NI V +     +EG  N T L E  +++GF+V+  ++    C +C+ NGG C  +  
Sbjct: 189 GIFNITVPIGY-APEEGALNVTNL-ESVLKKGFEVKL-SIDERPCQECKTNGGICAYHVA 248

Query: 487 YPFYCICRSGEIHPYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKMNAMAGVGCG-- 546
            P  C   S                 S   + T ++ + G+S +         G+G    
Sbjct: 249 TPVCCKTNS-----------------SSEVNCTPMMPS-GSSAHAGLSKKGKIGIGFASG 308

Query: 547 -IGGIILMGTISLILLRLQK------NKHAHASSFPLGNNY-----------------FD 606
            +G  ++ G +  I +R +K           +++ P  +NY                   
Sbjct: 309 FLGATLIGGCLLCIFIRRRKKLATQYTNKGLSTTTPYSSNYTMSNTPTSTTISGSNHSLV 368

Query: 607 PPSKELEKGENYMGVPLFSYGELEEATDKFNPAKELGDGGFGTVYYGKLRDGREVAVKRL 666
           P    L  G  Y G+ +FSY ELEEAT+ F  +KELGDGGFGTVYYG L+DGR VAVKRL
Sbjct: 369 PSISNLGNGSVYSGIQVFSYEELEEATENF--SKELGDGGFGTVYYGTLKDGRAVAVKRL 428

Query: 667 FENNYRKVEHFMNEVKILTCLHHQHLVTLYGCTSRRSRELLLVYEFIPNGTLADHLHGNR 726
           FE + ++VE F NE+ IL  L H +LV LYGCT+R SRELLLVYE+I NGTLA+HLHGN+
Sbjct: 429 FERSLKRVEQFKNEIDILKSLKHPNLVILYGCTTRHSRELLLVYEYISNGTLAEHLHGNQ 488

Query: 727 AKSGELPWNTRLKIAIETASALAYLHASKTIHRDVKTTNILLDKNFCVKVADLGLSCLSP 786
           A+S  + W  RL+IAIETASAL+YLHAS  IHRDVKTTNILLD N+ VKVAD GLS L P
Sbjct: 489 AQSRPICWPARLQIAIETASALSYLHASGIIHRDVKTTNILLDSNYQVKVADFGLSRLFP 548

Query: 787 TQATHVSTAPQGTPGYVDPEYQECYQLTNKSDVFSFGVVLVELISSKPAVDVTRHRHEIN 846
              TH+STAPQGTPGYVDPEY +CY+L  KSDV+SFGVVL ELISSK AVD+TRHRH+IN
Sbjct: 549 MDQTHISTAPQGTPGYVDPEYYQCYRLNEKSDVYSFGVVLSELISSKEAVDITRHRHDIN 608

Query: 847 LWTMAINKIQNNDLHNFVDPSLRFETDQSVRDMIYAAAELAFRCLQSVKDMRPSMLEALE 884
           L  MAI+KIQN+ +H   D SL F  D SV+ M+ + AELAFRCLQ  +D+RPSM E +E
Sbjct: 609 LANMAISKIQNDAVHELADLSLGFARDPSVKKMMSSVAELAFRCLQQERDVRPSMDEIVE 634

BLAST of MS021656 vs. ExPASy Swiss-Prot
Match: Q9C6K9 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.1 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.1 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 3.1e-114
Identity = 212/348 (60.92%), Postives = 262/348 (75.29%), Query Frame = 0

Query: 538 VGCGIGGIILMGTISLILLRLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMGVPLFSY 597
           +G GIGG +++  I + L  +    +       L     D    ++E  + +  +P+FSY
Sbjct: 225 LGLGIGGSVILIIILVALFAVIHRNYRRKDGSELSR---DNSKSDVEFSQVFFKIPIFSY 284

Query: 598 GELEEATDKFNPAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTC 657
            EL+ ATD F+  + LGDGGFGTVYYGK+RDGREVAVKRL+E+NYR++E FMNE++ILT 
Sbjct: 285 KELQAATDNFSKDRLLGDGGFGTVYYGKVRDGREVAVKRLYEHNYRRLEQFMNEIEILTR 344

Query: 658 LHHQHLVTLYGCTSRRSRELLLVYEFIPNGTLADHLHG-NRAKSGELPWNTRLKIAIETA 717
           LHH++LV+LYGCTSRRSRELLLVYEFIPNGT+ADHL+G N    G L W+ RL IAIETA
Sbjct: 345 LHHKNLVSLYGCTSRRSRELLLVYEFIPNGTVADHLYGENTPHQGFLTWSMRLSIAIETA 404

Query: 718 SALAYLHASKTIHRDVKTTNILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDP 777
           SALAYLHAS  IHRDVKTTNILLD+NF VKVAD GLS L P+  THVSTAPQGTPGYVDP
Sbjct: 405 SALAYLHASDIIHRDVKTTNILLDRNFGVKVADFGLSRLLPSDVTHVSTAPQGTPGYVDP 464

Query: 778 EYQECYQLTNKSDVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVD 837
           EY  CY LT+KSDV+SFGVVLVELISSKPAVD++R + EINL ++AINKIQN+  H  +D
Sbjct: 465 EYHRCYHLTDKSDVYSFGVVLVELISSKPAVDISRCKSEINLSSLAINKIQNHATHELID 524

Query: 838 PSLRFETDQSVRDMIYAAAELAFRCLQSVKDMRPSMLEALEILKNIES 885
            +L + T++ VR M    AELAF+CLQ    MRP+M + +  LK I++
Sbjct: 525 QNLGYATNEGVRKMTTMVAELAFQCLQQDNTMRPTMEQVVHELKGIQN 569

BLAST of MS021656 vs. ExPASy Swiss-Prot
Match: Q9FF31 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.1 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.5e-76
Identity = 137/227 (60.35%), Postives = 170/227 (74.89%), Query Frame = 0

Query: 4   LGQGGFSTVYKGKLPDGRDVAVKLLNESKENGQDFINEVVSITKTSHVNIATLLGFCYER 63
           LG+GGF TVY G L DGR VAVK+L + K NG+DFINEV S+++TSHVNI +LLGFCYE 
Sbjct: 327 LGKGGFGTVYGGNLCDGRKVAVKILKDFKSNGEDFINEVASMSQTSHVNIVSLLGFCYEG 386

Query: 64  NRRALVYEYMPKGSLDKYIFHKDPQKSDMGLDWNTLYTIVIGVARGLEYLHRGCNTRILH 123
           ++RA+VYE++  GSLD+++     +K  + LD +TLY I +GVARGL+YLH GC TRI+H
Sbjct: 387 SKRAIVYEFLENGSLDQFL----SEKKSLNLDVSTLYRIALGVARGLDYLHHGCKTRIVH 446

Query: 124 FDIKPHNILLDNEFCPKISDFGLAKQCRARESHVSMTGVKGTIGFMAPEVVFRNIGKVSH 183
           FDIKP NILLD+ FCPK+SDFGLAK C  RES +S+   +GTIG++APEV     G+VSH
Sbjct: 447 FDIKPQNILLDDTFCPKVSDFGLAKLCEKRESILSLLDARGTIGYIAPEVFSGMYGRVSH 506

Query: 184 KSDVYSYGMLVLEMVGERKNPNE--GVGQRSEEYFPDWIYKDLTQSE 229
           KSDVYSYGMLVLEM+G +    E       S  YFPDWIYK+L   E
Sbjct: 507 KSDVYSYGMLVLEMIGAKNKEIEETAASNSSSAYFPDWIYKNLENGE 549

BLAST of MS021656 vs. ExPASy TrEMBL
Match: A0A6J1C7V1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111008789 OS=Momordica charantia OX=3673 GN=LOC111008789 PE=4 SV=1)

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 593/638 (92.95%), Postives = 604/638 (94.67%), Query Frame = 0

Query: 249 SASSSSANVFFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRRE 308
           S S++ +NVFFIAFHLSLCFTVDAIDEFKACGHSYNCGELV+IAYPFWGNERKGFCGRRE
Sbjct: 4   SQSTTISNVFFIAFHLSLCFTVDAIDEFKACGHSYNCGELVNIAYPFWGNERKGFCGRRE 63

Query: 309 FELKCKHNRTTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFV 368
           FELKCKHNRTTTIQINSLEYN+LKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFV
Sbjct: 64  FELKCKHNRTTTIQINSLEYNILKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFV 123

Query: 369 YSSNDQNISVRYNCSAQKEIPETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEECTMNI 428
           YSSNDQNISVRYNCSAQKEIPETYKFSCG EEEKNGRANYAFEPSAATWNLLIEECTMNI
Sbjct: 124 YSSNDQNISVRYNCSAQKEIPETYKFSCGSEEEKNGRANYAFEPSAATWNLLIEECTMNI 183

Query: 429 DVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYC 488
           DVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYC
Sbjct: 184 DVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYC 243

Query: 489 ICRSGEIHPYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKMNAMAGVGCGIGGIILM 548
           ICRSGEIHPYFCGTAHAL   + ++                   N + GVGCGIGGIILM
Sbjct: 244 ICRSGEIHPYFCGTAHALEESNDKW------------------KNTVIGVGCGIGGIILM 303

Query: 549 GTISLILLRLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMGVPLFSYGELEEATDKFN 608
           GTISLILLRLQKNKHAHASSFPLGNNYFDPPSKELEKGENYM VPLFSYGELEEATDKFN
Sbjct: 304 GTISLILLRLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMVVPLFSYGELEEATDKFN 363

Query: 609 PAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYG 668
           PAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYG
Sbjct: 364 PAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYG 423

Query: 669 CTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIETASALAYLHASKTI 728
           CTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPW TRLKIAIETASALAYLHASKTI
Sbjct: 424 CTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPWITRLKIAIETASALAYLHASKTI 483

Query: 729 HRDVKTTNILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDPEYQECYQLTNKS 788
           HRDVKTTNILLDKN  VKVADLGLSCLSPTQATHVSTAPQGTPGY+DPEYQECYQLTNKS
Sbjct: 484 HRDVKTTNILLDKNLSVKVADLGLSCLSPTQATHVSTAPQGTPGYIDPEYQECYQLTNKS 543

Query: 789 DVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVR 848
           DVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVR
Sbjct: 544 DVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVR 603

Query: 849 DMIYAAAELAFRCLQSVKDMRPSMLEALEILKNIESRS 887
           DMIYA A LAF+CLQSVKDMRPSMLEALEILKNIESRS
Sbjct: 604 DMIYAVAGLAFQCLQSVKDMRPSMLEALEILKNIESRS 623

BLAST of MS021656 vs. ExPASy TrEMBL
Match: A0A6J1C9Y1 (uncharacterized protein LOC111008790 OS=Momordica charantia OX=3673 GN=LOC111008790 PE=4 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 3.5e-262
Identity = 507/906 (55.96%), Postives = 593/906 (65.45%), Query Frame = 0

Query: 1    KNKLGQGGFSTVYKGKLPDGRDVAVKLLNESKENGQDFINEVVSITKTSHVNIATLLGFC 60
            KNKLGQGGFSTVYKGKLPDGRDVAVKLLNESKENGQDFINEVVSIT TSHVNIATLLGFC
Sbjct: 335  KNKLGQGGFSTVYKGKLPDGRDVAVKLLNESKENGQDFINEVVSITTTSHVNIATLLGFC 394

Query: 61   YERNRRALVYEYMPKGSLDKYIFHKDPQKSDMGLDWNTLYTIVIGVARGLEYLHRGCNTR 120
            YERNRRALVYEYMPKGSLDKYIFHKDPQKSDMGLDWNTLYTIVIGVARGLEYLHRGCNTR
Sbjct: 395  YERNRRALVYEYMPKGSLDKYIFHKDPQKSDMGLDWNTLYTIVIGVARGLEYLHRGCNTR 454

Query: 121  ILHFDIKPHNILLDNEFCPKISDFGLAKQCRARESHVSMTGVKGTIGFMAPEVVFRNIGK 180
            ILHFDIKPHNILLDNEFCPKISDFGLAKQCRARESHVSMTGVKGTIGFMAPEVVFRNIGK
Sbjct: 455  ILHFDIKPHNILLDNEFCPKISDFGLAKQCRARESHVSMTGVKGTIGFMAPEVVFRNIGK 514

Query: 181  VSHKSDVYSYGMLVLEMVGERKNPNEGVGQRSEEYFPDWIYKDLTQSETDEG--W----- 240
            VSHKSDVYSYGMLVLEMVGERKNPNEGVGQRSEEYFPDWIYKDLTQSETDEG  W     
Sbjct: 515  VSHKSDVYSYGMLVLEMVGERKNPNEGVGQRSEEYFPDWIYKDLTQSETDEGCSWGNTEE 574

Query: 241  -----------------------------------NADALQIPPKPNLFGS--------- 300
                                               + D LQIPPKPN  G+         
Sbjct: 575  EDEMARKMIIVGLHCIQTLPDDRPSMSDVVGMLEGSVDGLQIPPKPNFMGTPTTTSTTLP 634

Query: 301  --ASSSSAN-----------------------------------VFFIAFHLSLCFTVDA 360
              +SSS +N                                   + FIAFHLS  F    
Sbjct: 635  HPSSSSPSNFLMNPVVQKLNKMRIIHLSLTSFGLNHTFIGNFLFISFIAFHLSPSF---G 694

Query: 361  IDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRREFELKCKHNRTTTIQINSLEYNVLK 420
             DEFKACG SYNCGELV+I YPFWGN+R+GFCGRREFEL CK NRTTTIQINS +YNVL+
Sbjct: 695  DDEFKACGISYNCGELVNITYPFWGNKRQGFCGRREFELNCKRNRTTTIQINSFDYNVLR 754

Query: 421  INQSDHRMTIARSDLF--DNLCPKNQTQTATLDHHLFVYSSNDQNISVRYNCSAQKEIPE 480
            INQ+DHRMTIARSDL    NLCPKN+T TA LD+++F YS ND  IS+ Y+C  Q E  E
Sbjct: 755  INQTDHRMTIARSDLLVPGNLCPKNKTGTANLDYNVFRYSENDLQISLWYDCPLQGENVE 814

Query: 481  TYKFSCGLEEEKNGRANYAFEPSAATWNLLIE-ECTMNIDVKVTMEGLKEGL-KNRTRLV 540
            +Y+FSCG E EKNG  NYAF+     W    E +C  N++VKV  EG + G  KNRT  V
Sbjct: 815  SYRFSCGGEGEKNGSGNYAFDAMQTIWRNQFEGKCRTNVEVKVRREGWEGGNGKNRTGAV 874

Query: 541  EKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYCICRSGEIHPYFCGTAHALGT 600
            E A+R GFDVEYGNLY+  C  C+ +GG CGGNGTYPFYCIC S +  P  C  A A G 
Sbjct: 875  ESALRGGFDVEYGNLYSTECEGCKESGGKCGGNGTYPFYCICESQDAQPSACRAAAAPGV 934

Query: 601  FSPRFHITYIIITFGNSTNLTRKMNAMAGVGCGIGGIILMGTISLILLRLQKNKHAHASS 660
             S RF     II                 V   +GGII + ++ +I++   +N       
Sbjct: 935  -SYRFPKKISII-----------------VAIVLGGIISIISLVVIIIYHTRNSIPKDKI 994

Query: 661  FPLGNNYFDPPSKELEKGENYMGVPLFSYGELEEATDKFNPAKELGDGGFGTVYYGKLRD 720
              + + Y     K             +SY +L++ TD FN   +LG GGF TVY GKL D
Sbjct: 995  DEIISRYSTHTPKR------------YSYSKLKKITDSFN--NKLGQGGFSTVYRGKLPD 1054

Query: 721  GREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYG-CTSRRSRELLLVYEFIPNG 780
            GR+VAVK L E+  +  + FMNEV  +T   H ++V++ G C  R  R   L+YE++P G
Sbjct: 1055 GRDVAVKLLNESRDQNGQDFMNEVVSITRTSHVNIVSILGFCYERNKR--ALIYEYMPKG 1114

Query: 781  TLAD---HLHGNRAKSGELPWNTRLKIAIETASALAYLHA---SKTIHRDVKTTNILLDK 805
            +L     H    R+   +L WNT   IA+  A  L YLH    ++ +H D+K  NILLD 
Sbjct: 1115 SLDKYIFHKDPQRSNVEKLDWNTVYSIAVGVARGLEYLHQGCNTRILHFDIKPHNILLDN 1174

BLAST of MS021656 vs. ExPASy TrEMBL
Match: A0A6J1KES1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495165 PE=4 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 6.7e-245
Identity = 438/630 (69.52%), Postives = 498/630 (79.05%), Query Frame = 0

Query: 258 FFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRREFELKCKHNR 317
           F   FHLS CF+ +A+D FK CG SYNCGELV+I YPFWGNE++  CGR+EF+L CK NR
Sbjct: 8   FLFTFHLSTCFSDNALDAFKDCGVSYNCGELVNITYPFWGNEKQSSCGRKEFKLNCKSNR 67

Query: 318 TTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFVYSSNDQNIS 377
           TTT  INSLEYNVL+INQS++ M IARSDLFDN CP+NQ Q A++DHH F YSS +QNIS
Sbjct: 68  TTTTYINSLEYNVLEINQSNNTMRIARSDLFDNYCPRNQIQVASIDHHPFAYSSRNQNIS 127

Query: 378 VRYNCSAQKE-IPETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEECTMNIDVKVTMEG 437
           V YNCS   E +   YKFSCG E ++ GR NYAFEPSAA ++ L  EC M I+V VT++ 
Sbjct: 128 VGYNCSPDNEFLISKYKFSCGGEWQRRGRVNYAFEPSAAVYSKL-AECEMKIEVMVTIKE 187

Query: 438 LKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYCICRSGEIH 497
           LKEGLKNRT LVE AV+ GF+VEY N Y  AC+ C  +GGNCGGNGTYPFYCICR+   +
Sbjct: 188 LKEGLKNRTSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRNEVAN 247

Query: 498 PYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKMNAMAGVGCGIGGIILMGTISLILL 557
           PY C    A     PR            + N T K   + G G GIGGI++M  I  I L
Sbjct: 248 PYACDAIAA----PPR-------PPHPENPNDTWK-KVVIGAGSGIGGIVMMSIIFFIRL 307

Query: 558 RLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMGVPLFSYGELEEATDKFNPAKELGDG 617
           R+ K KH + S         D P KELEK E+YM + LFSY EL +ATDKFNPA ELGDG
Sbjct: 308 RMHKKKHQNYSC--------DRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGDG 367

Query: 618 GFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYGCTSRRSRE 677
           GFGTVYYGKLRDGREVAVKRLFENNYRKVE FMNEV++LT L H HLVTLYGCTSRRSRE
Sbjct: 368 GFGTVYYGKLRDGREVAVKRLFENNYRKVEQFMNEVEMLTRLRHPHLVTLYGCTSRRSRE 427

Query: 678 LLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIETASALAYLHASKTIHRDVKTTN 737
           LLLVY+FIPNGT+ADHLHGNRA+SGELPW TRLK+AIETASALA+LHAS+TIHRDVKTTN
Sbjct: 428 LLLVYKFIPNGTVADHLHGNRARSGELPWLTRLKVAIETASALAFLHASETIHRDVKTTN 487

Query: 738 ILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDPEYQECYQLTNKSDVFSFGVV 797
           ILLD NF VKVAD GLS L PTQA+HVSTAPQGTPGY+DPEY E YQLTNKSDVFSFGVV
Sbjct: 488 ILLDNNFGVKVADFGLSRLFPTQASHVSTAPQGTPGYIDPEYYESYQLTNKSDVFSFGVV 547

Query: 798 LVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVRDMIYAAAE 857
           LVELISSKPAVD+TRHRHEINL TMAINKIQN++LH+FVDPSL F+TDQ +R+MI   AE
Sbjct: 548 LVELISSKPAVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKTDQRIRNMICGVAE 607

Query: 858 LAFRCLQSVKDMRPSMLEALEILKNIESRS 887
           LAF+CLQSV+D RPSM+EALEIL+NIE+RS
Sbjct: 608 LAFQCLQSVRDTRPSMMEALEILRNIENRS 616

BLAST of MS021656 vs. ExPASy TrEMBL
Match: A0A6J1KJE6 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495165 PE=4 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 1.1e-244
Identity = 439/631 (69.57%), Postives = 501/631 (79.40%), Query Frame = 0

Query: 258 FFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRREFELKCKHNR 317
           F   FHLS CF+ +A+D FK CG SYNCGELV+I YPFWGNE++  CGR+EF+L CK NR
Sbjct: 8   FLFTFHLSTCFSDNALDAFKDCGVSYNCGELVNITYPFWGNEKQSSCGRKEFKLNCKSNR 67

Query: 318 TTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFVYSSNDQNIS 377
           TTT  INSLEYNVL+INQS++ M IARSDLFDN CP+NQ Q A++DHH F YSS +QNIS
Sbjct: 68  TTTTYINSLEYNVLEINQSNNTMRIARSDLFDNYCPRNQIQVASIDHHPFAYSSRNQNIS 127

Query: 378 VRYNCSAQKE-IPETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEECTMNIDVKVTMEG 437
           V YNCS   E +   YKFSCG E ++ GR NYAFEPSAA ++ L  EC M I+V VT++ 
Sbjct: 128 VGYNCSPDNEFLISKYKFSCGGEWQRRGRVNYAFEPSAAVYSKL-AECEMKIEVMVTIKE 187

Query: 438 LKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYCICRSGEIH 497
           LKEGLKNRT LVE AV+ GF+VEY N Y  AC+ C  +GGNCGGNGTYPFYCICR+   +
Sbjct: 188 LKEGLKNRTSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRNEVAN 247

Query: 498 PYFCGTAHALGTFSPR-FHITYIIITFGNSTNLTRKMNAMAGVGCGIGGIILMGTISLIL 557
           PY C    A     PR  H ++       + N T K   + G G GIGGI++M  I  I 
Sbjct: 248 PYACDAIAA----PPRPPHPSH-----PENPNDTWK-KVVIGAGSGIGGIVMMSIIFFIR 307

Query: 558 LRLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMGVPLFSYGELEEATDKFNPAKELGD 617
           LR+ K KH + S         D P KELEK E+YM + LFSY EL +ATDKFNPA ELGD
Sbjct: 308 LRMHKKKHQNYSC--------DRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGD 367

Query: 618 GGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYGCTSRRSR 677
           GGFGTVYYGKLRDGREVAVKRLFENNYRKVE FMNEV++LT L H HLVTLYGCTSRRSR
Sbjct: 368 GGFGTVYYGKLRDGREVAVKRLFENNYRKVEQFMNEVEMLTRLRHPHLVTLYGCTSRRSR 427

Query: 678 ELLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIETASALAYLHASKTIHRDVKTT 737
           ELLLVY+FIPNGT+ADHLHGNRA+SGELPW TRLK+AIETASALA+LHAS+TIHRDVKTT
Sbjct: 428 ELLLVYKFIPNGTVADHLHGNRARSGELPWLTRLKVAIETASALAFLHASETIHRDVKTT 487

Query: 738 NILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDPEYQECYQLTNKSDVFSFGV 797
           NILLD NF VKVAD GLS L PTQA+HVSTAPQGTPGY+DPEY E YQLTNKSDVFSFGV
Sbjct: 488 NILLDNNFGVKVADFGLSRLFPTQASHVSTAPQGTPGYIDPEYYESYQLTNKSDVFSFGV 547

Query: 798 VLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVRDMIYAAA 857
           VLVELISSKPAVD+TRHRHEINL TMAINKIQN++LH+FVDPSL F+TDQ +R+MI   A
Sbjct: 548 VLVELISSKPAVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKTDQRIRNMICGVA 607

Query: 858 ELAFRCLQSVKDMRPSMLEALEILKNIESRS 887
           ELAF+CLQSV+D RPSM+EALEIL+NIE+RS
Sbjct: 608 ELAFQCLQSVRDTRPSMMEALEILRNIENRS 619

BLAST of MS021656 vs. ExPASy TrEMBL
Match: A0A6J1E6Z3 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=Cucurbita moschata OX=3662 GN=LOC111431355 PE=4 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 3.3e-244
Identity = 434/630 (68.89%), Postives = 496/630 (78.73%), Query Frame = 0

Query: 258 FFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGRREFELKCKHNR 317
           FF  FHLS CF+ +A+D FK CG +YNC ELV+I YPFWGNER+  CGR+EF+L C +NR
Sbjct: 42  FFFTFHLSPCFSDNALDAFKDCGVNYNCEELVNITYPFWGNERQSSCGRKEFKLNCNNNR 101

Query: 318 TTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFVYSSNDQNIS 377
           TTT  INSLEYNVL+INQ ++ M IARSDLFDN CP+NQ Q A++DHH FVYS+ +QNIS
Sbjct: 102 TTTTYINSLEYNVLEINQLNNTMRIARSDLFDNYCPRNQIQVASMDHHPFVYSARNQNIS 161

Query: 378 VRYNCSAQKEIP-ETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEECTMNIDVKVTMEG 437
           V YNCS   E P   Y FSCG E E+ GR NYAFEPSAA ++ L+ EC M I+V VT++G
Sbjct: 162 VGYNCSTDNEFPISEYNFSCGGEWERRGRVNYAFEPSAAVYSKLV-ECEMKIEVMVTIKG 221

Query: 438 LKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYCICRSGEIH 497
           LKEG+KNR  LVE AV+ GF+VEY N Y  AC+ C  +GGNCGGNGTYPFYCICR+G  +
Sbjct: 222 LKEGVKNRRSLVETAVKGGFEVEYENWYKGACDSCNGSGGNCGGNGTYPFYCICRNGVAN 281

Query: 498 PYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKMNAMAGVGCGIGGIILMGTISLILL 557
           PY C    A     PR            + N T K   + G G GIGGI++M  I  I L
Sbjct: 282 PYACDATAA----PPR-------PPHPENPNDTWK-KVVIGAGSGIGGIVMMSIIFFIRL 341

Query: 558 RLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMGVPLFSYGELEEATDKFNPAKELGDG 617
           R+ K KH + S         D P KELEK E+YM + LFSY EL +ATDKFNPA ELGDG
Sbjct: 342 RMHKKKHQNYSC--------DRPMKELEKIEHYMALSLFSYHELVKATDKFNPANELGDG 401

Query: 618 GFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTCLHHQHLVTLYGCTSRRSRE 677
           GFGTVYYGKLRDGREVAVKRLFENNYRKVE FMNEV++LT L H HLVTLYGCTSRRSRE
Sbjct: 402 GFGTVYYGKLRDGREVAVKRLFENNYRKVEQFMNEVEMLTRLRHPHLVTLYGCTSRRSRE 461

Query: 678 LLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIETASALAYLHASKTIHRDVKTTN 737
           LLLVYEFIPNGT+ADHLHGNRA+SGELPW TRL +AIETASAL++LHAS+TIHRDVKTTN
Sbjct: 462 LLLVYEFIPNGTVADHLHGNRARSGELPWLTRLNVAIETASALSFLHASETIHRDVKTTN 521

Query: 738 ILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDPEYQECYQLTNKSDVFSFGVV 797
           ILLD NF VKVAD GL  L PTQA+HVSTAPQGTPGY+DPEY E YQLTNKSDVFSFGVV
Sbjct: 522 ILLDNNFGVKVADFGLCRLFPTQASHVSTAPQGTPGYIDPEYYESYQLTNKSDVFSFGVV 581

Query: 798 LVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDPSLRFETDQSVRDMIYAAAE 857
           LVELISSKPAVD+TRHRHEINL TMAINKIQN++LH+FVDPSL F+TDQ +R+MI   AE
Sbjct: 582 LVELISSKPAVDITRHRHEINLSTMAINKIQNDELHDFVDPSLGFKTDQRIRNMICGVAE 641

Query: 858 LAFRCLQSVKDMRPSMLEALEILKNIESRS 887
           LAF+CLQS +D RPSM+EALEIL+NIE+RS
Sbjct: 642 LAFQCLQSARDTRPSMMEALEILRNIENRS 650

BLAST of MS021656 vs. TAIR 10
Match: AT1G18390.1 (Protein kinase superfamily protein )

HSP 1 Score: 476.5 bits (1225), Expect = 4.7e-134
Identity = 286/650 (44.00%), Postives = 379/650 (58.31%), Query Frame = 0

Query: 258 FFIAF------HLSLCFTVDAIDEFKAC-GHSYNCGE----LVSIAYPFWGNERK-GFCG 317
           FFI+F       L  CF+ D   +++ C   +  CG       S  YPFWG   K  FCG
Sbjct: 6   FFISFVVFSVADLPSCFSAD--QQYEECRSRNLTCGSGHRVFESTTYPFWGGFNKPKFCG 65

Query: 318 RREFELKCKHNRTTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLC-------PKNQTQ 377
              F+L C+ ++  T+ I ++   V+  N  DH++++A   L D  C        KNQ  
Sbjct: 66  HSSFKLSCEGDQNLTLAIGNITLRVVSANLEDHKISVADDSLLDGGCLNIWNFNGKNQ-- 125

Query: 378 TATLDHHLFVYSSNDQNISVRYNCSAQKEIPETYKFSCGLEEEKNGRANYAFEPSAATWN 437
                   F   SN + I V  NCS    +    + SC  EE       Y    S+ +  
Sbjct: 126 --------FTLDSNTETIDVFVNCSGVAPL----QISC--EESYEDPVTYHVLRSSDS-- 185

Query: 438 LLIEECTMNIDVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNC 497
              E C    ++ + +   K+ L+       +A+R+GFD+ Y  +   AC  C  +GG C
Sbjct: 186 --DEGCMKYAEIPM-LRSAKDELQRSELTFVEALRKGFDLRY-IMEDKACRRCIDSGGIC 245

Query: 498 GGN-GTYPFYCICRSGEIHPYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKMNAMAG 557
           G    +  F C+C +   H   C      G    R  +                +  + G
Sbjct: 246 GSALDSESFRCLC-ADRPHNSSCDDNTNQGKNDKRRRVI---------------VKVLIG 305

Query: 558 VGCGIGGIILMGTISLILLRLQKNKHAHASSFPLGNNYFDPPSK--ELEKGENYM-GVPL 617
               + G+I       +  R +   + ++S+    N   DP +K  ++EK E  + GV +
Sbjct: 306 ASAAVVGLIAASIFWYVYHRRKTKSYRNSSALLPRNISSDPSAKSFDIEKAEELLVGVHI 365

Query: 618 FSYGELEEATDKFNPAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKI 677
           FSY ELEEAT+ F+P+KELGDGGFGTVYYGKL+DGR VAVKRL++NN+++ E F NEV+I
Sbjct: 366 FSYEELEEATNNFDPSKELGDGGFGTVYYGKLKDGRSVAVKRLYDNNFKRAEQFRNEVEI 425

Query: 678 LTCLHHQHLVTLYGCTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIE 737
           LT L H +LV L+GC+S++SR+LLLVYE++ NGTLADHLHG +A    LPW+ RLKIA+E
Sbjct: 426 LTGLRHPNLVALFGCSSKQSRDLLLVYEYVANGTLADHLHGPQANPSSLPWSIRLKIAVE 485

Query: 738 TASALAYLHASKTIHRDVKTTNILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYV 797
           TASAL YLHASK IHRDVK+ NILLD+NF VKVAD GLS L P   THVSTAPQGTPGYV
Sbjct: 486 TASALKYLHASKIIHRDVKSNNILLDQNFNVKVADFGLSRLFPMDKTHVSTAPQGTPGYV 545

Query: 798 DPEYQECYQLTNKSDVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNF 857
           DP+Y  CYQL+NKSDV+SF VVL+ELISS PAVD+TR R EINL  MA+ KIQN++L + 
Sbjct: 546 DPDYHLCYQLSNKSDVYSFAVVLMELISSLPAVDITRPRQEINLSNMAVVKIQNHELRDM 605

Query: 858 VDPSLRFETDQSVRDMIYAAAELAFRCLQSVKDMRPSMLEALEILKNIES 885
           VDPSL F+TD  VR  + A AELAF+CLQS KD+RP M    + L  I++
Sbjct: 606 VDPSLGFDTDTRVRQTVIAVAELAFQCLQSDKDLRPCMSHVQDTLTRIQN 615

BLAST of MS021656 vs. TAIR 10
Match: AT1G18390.2 (Protein kinase superfamily protein )

HSP 1 Score: 457.2 bits (1175), Expect = 3.0e-128
Identity = 279/647 (43.12%), Postives = 377/647 (58.27%), Query Frame = 0

Query: 259 FIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFW-GNERKGFCGRREFELKC-KHN 318
           F  F +    T+    +FKAC    +CG+   I+YPF+   +++ FCG   FEL C    
Sbjct: 14  FFYFTIIATQTLSLDPKFKAC-EPKSCGKGPQISYPFYLSGKQESFCGYPSFELTCDDEE 73

Query: 319 RTTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHHLFVYSSNDQNI 378
           +   + I+  EY +  I+       +  S    + CP+      TL    F  + +  N 
Sbjct: 74  KLPVLGISGEEYVIKNISYLTQSFQVVNSKASHDPCPR-PLNNLTLHRTPFFVNPSHINF 133

Query: 379 SVRYNCSAQ-KEIPETYKFSCG-----------LEEEKNGRANYAFEPSAATWNLLIEEC 438
           ++ YNCS    E   TY  +C             + +K G+     E   A+ +     C
Sbjct: 134 TILYNCSDHLLEDFRTYPLTCARNTSLLRSFGVFDRKKLGK-----EKQIASMS-----C 193

Query: 439 TMNIDVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTY 498
              +DV V +   +  +   T +  + ++RGF +   N    +C  C  +GG C G    
Sbjct: 194 QKLVDVPV-LASNESDVMGMTYV--EILKRGFVL---NWTANSCFRCITSGGRC-GTDQQ 253

Query: 499 PFYCICRSGEIHPYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKM----NAMAGVGC 558
            F C+C  G                 P+ H T    T G +    R +     +++G   
Sbjct: 254 EFVCLCPDG-----------------PKLHDT---CTNGKNDKRRRVIVKITKSISGASA 313

Query: 559 GIGGIILMGTISLILLRLQKNKHAHASSFPLGNNYFDPPSK--ELEKGENYM-GVPLFSY 618
            + G+I       +  R +   + ++S+    N   DP +K  ++EK E  + GV +FSY
Sbjct: 314 AVVGLIAASIFWYVYHRRKTKSYRNSSALLPRNISSDPSAKSFDIEKAEELLVGVHIFSY 373

Query: 619 GELEEATDKFNPAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTC 678
            ELEEAT+ F+P+KELGDGGFGTVYYGKL+DGR VAVKRL++NN+++ E F NEV+ILT 
Sbjct: 374 EELEEATNNFDPSKELGDGGFGTVYYGKLKDGRSVAVKRLYDNNFKRAEQFRNEVEILTG 433

Query: 679 LHHQHLVTLYGCTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAIETAS 738
           L H +LV L+GC+S++SR+LLLVYE++ NGTLADHLHG +A    LPW+ RLKIA+ETAS
Sbjct: 434 LRHPNLVALFGCSSKQSRDLLLVYEYVANGTLADHLHGPQANPSSLPWSIRLKIAVETAS 493

Query: 739 ALAYLHASKTIHRDVKTTNILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDPE 798
           AL YLHASK IHRDVK+ NILLD+NF VKVAD GLS L P   THVSTAPQGTPGYVDP+
Sbjct: 494 ALKYLHASKIIHRDVKSNNILLDQNFNVKVADFGLSRLFPMDKTHVSTAPQGTPGYVDPD 553

Query: 799 YQECYQLTNKSDVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVDP 858
           Y  CYQL+NKSDV+SF VVL+ELISS PAVD+TR R EINL  MA+ KIQN++L + VDP
Sbjct: 554 YHLCYQLSNKSDVYSFAVVLMELISSLPAVDITRPRQEINLSNMAVVKIQNHELRDMVDP 613

Query: 859 SLRFETDQSVRDMIYAAAELAFRCLQSVKDMRPSMLEALEILKNIES 885
           SL F+TD  VR  + A AELAF+CLQS KD+RP M    + L  I++
Sbjct: 614 SLGFDTDTRVRQTVIAVAELAFQCLQSDKDLRPCMSHVQDTLTRIQN 621

BLAST of MS021656 vs. TAIR 10
Match: AT1G66880.1 (Protein kinase superfamily protein )

HSP 1 Score: 450.3 bits (1157), Expect = 3.6e-126
Identity = 269/650 (41.38%), Postives = 373/650 (57.38%), Query Frame = 0

Query: 277  KACGHSYNCGELVSIAYPFWGNERKGFCGRREFELKCKHNRTTTIQINSLEYNVLKINQS 336
            + C  +++CG+   + YPFW + R+  CG  +F+L     R   + I+S+++ +L     
Sbjct: 629  RRCNGTFSCGDQRELFYPFWTSGRED-CGHPDFKLDDCSGRFAELSISSVKFRILASVYG 688

Query: 337  DHRMTIARSDLFDNLCPKNQTQTATLDHHLFVYSSNDQNISVRYNCSAQ--KEIPETYKF 396
             + + + RS+   +LCP++    A     +  ++ N + +++ YNCS    +++     F
Sbjct: 689  SNIIRLGRSEYIGDLCPQDPI-NAPFSESVLPFAPNTELLTIFYNCSRDFPQQVTNFGDF 748

Query: 397  SCGLEEEKNG----RANYAFEPSAATWNLLI---EECTMNIDVKV---TMEGLKEGLKNR 456
            +CG + + +       N +F P +   +LL    + C  N+ +     T+  L+    N 
Sbjct: 749  ACGDDSDDDRSYYVTRNLSFPPLSEINDLLYDFSQSCDRNVSIPASGSTLNILQSTPSND 808

Query: 457  TRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGTYPFYCICRSGEIHPYFCGTAH 516
               ++KA+  GF++E        C  C  + G CG + T              + C +  
Sbjct: 809  N--LKKALEYGFELELNQ----DCRTCIDSKGACGYSQT-----------SSRFVCYSIE 868

Query: 517  ALGTFSPRFHITYIIITFGNSTNLTRKMNAMAGVGCGIG---------GIILMGTISLIL 576
               T +P               N TR  +    +G   G          I+L+  + L +
Sbjct: 869  EPQTPTP--------------PNPTRNKDTSLSIGAKAGIAVASVSGLAILLLAGLFLCI 928

Query: 577  LRLQKNKHAH--ASSFPL--------------------GNNYFDPPSKELEKGENYMGVP 636
             R +K + A   + S P+                     N+   P    L    +Y GV 
Sbjct: 929  RRRRKTQDAQYTSKSLPITSYSSRDTSRNPTSTTISSSSNHSLLPSISNLANRSDYCGVQ 988

Query: 637  LFSYGELEEATDKFNPAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVK 696
            +FSY ELEEAT+ F  ++ELGDGGFGTVYYG L+DGR VAVKRL+E + ++VE F NE++
Sbjct: 989  VFSYEELEEATENF--SRELGDGGFGTVYYGVLKDGRAVAVKRLYERSLKRVEQFKNEIE 1048

Query: 697  ILTCLHHQHLVTLYGCTSRRSRELLLVYEFIPNGTLADHLHGNRAKSGELPWNTRLKIAI 756
            IL  L H +LV LYGCTSR SRELLLVYE+I NGTLA+HLHGNRA++  L W+TRL IAI
Sbjct: 1049 ILKSLKHPNLVILYGCTSRHSRELLLVYEYISNGTLAEHLHGNRAEARPLCWSTRLNIAI 1108

Query: 757  ETASALAYLHASKTIHRDVKTTNILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGY 816
            ETASAL++LH    IHRD+KTTNILLD N+ VKVAD GLS L P   TH+STAPQGTPGY
Sbjct: 1109 ETASALSFLHIKGIIHRDIKTTNILLDDNYQVKVADFGLSRLFPMDQTHISTAPQGTPGY 1168

Query: 817  VDPEYQECYQLTNKSDVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHN 876
            VDPEY +CYQL  KSDV+SFGVVL ELISSK AVD+TRHRH+INL  MA++KIQNN LH 
Sbjct: 1169 VDPEYYQCYQLNEKSDVYSFGVVLTELISSKEAVDITRHRHDINLANMAVSKIQNNALHE 1228

Query: 877  FVDPSLRFETDQSVRDMIYAAAELAFRCLQSVKDMRPSMLEALEILKNIE 884
             VD SL ++ D  VR  + A AELAFRCLQ  +D+RP+M E +EIL+ I+
Sbjct: 1229 LVDSSLGYDNDPEVRRKMMAVAELAFRCLQQERDVRPAMDEIVEILRGIK 1243

BLAST of MS021656 vs. TAIR 10
Match: AT5G38210.1 (Protein kinase family protein )

HSP 1 Score: 448.7 bits (1153), Expect = 1.1e-125
Identity = 283/666 (42.49%), Postives = 378/666 (56.76%), Query Frame = 0

Query: 247 FGSASSSSANVFFIAFHLSLCFTVDAIDEFKACGHSYNCGELVSIAYPFWGNERKGFCGR 306
           F   +S    +FF+++   + F   A  + + C   + CG+L +  +PFWG  R   CG 
Sbjct: 9   FSKPNSFLVLLFFLSY---IHFLPCAQSQREPCDTLFRCGDLTA-GFPFWGVARPQPCGH 68

Query: 307 REFELKC-KHNRTTTIQINSLEYNVLKINQSDHRMTIARSDLFDNLCPKNQTQTATLDHH 366
               L C K   +T++ I+SL Y VL++N +   + + R D     C  +    ATL   
Sbjct: 69  PSLGLHCQKQTNSTSLIISSLMYRVLEVNTTTSTLKLVRQDFSGPFCSAS-FSGATLTPE 128

Query: 367 LFVYSSNDQNISVRYNCSAQKEIPETYKFSCGLEEEKNGRANYAFEPSAATWNLLIEEC- 426
           LF    + + +S  Y C+     P   KF C       G  +   +      +L    C 
Sbjct: 129 LFELLPDYKTLSAYYLCNPSLHYPA--KFIC----PNKGVGSIHQD------DLYHNHCG 188

Query: 427 -TMNIDVKVTMEGLKEGLKNRTRLVEKAVRRGFDVEYGNLYTVACNDCERNGGNCGGNGT 486
              NI V +     +EG  N T L E  +++GF+V+  ++    C +C+ NGG C  +  
Sbjct: 189 GIFNITVPIGY-APEEGALNVTNL-ESVLKKGFEVKL-SIDERPCQECKTNGGICAYHVA 248

Query: 487 YPFYCICRSGEIHPYFCGTAHALGTFSPRFHITYIIITFGNSTNLTRKMNAMAGVGCG-- 546
            P  C   S                 S   + T ++ + G+S +         G+G    
Sbjct: 249 TPVCCKTNS-----------------SSEVNCTPMMPS-GSSAHAGLSKKGKIGIGFASG 308

Query: 547 -IGGIILMGTISLILLRLQK------NKHAHASSFPLGNNY-----------------FD 606
            +G  ++ G +  I +R +K           +++ P  +NY                   
Sbjct: 309 FLGATLIGGCLLCIFIRRRKKLATQYTNKGLSTTTPYSSNYTMSNTPTSTTISGSNHSLV 368

Query: 607 PPSKELEKGENYMGVPLFSYGELEEATDKFNPAKELGDGGFGTVYYGKLRDGREVAVKRL 666
           P    L  G  Y G+ +FSY ELEEAT+ F  +KELGDGGFGTVYYG L+DGR VAVKRL
Sbjct: 369 PSISNLGNGSVYSGIQVFSYEELEEATENF--SKELGDGGFGTVYYGTLKDGRAVAVKRL 428

Query: 667 FENNYRKVEHFMNEVKILTCLHHQHLVTLYGCTSRRSRELLLVYEFIPNGTLADHLHGNR 726
           FE + ++VE F NE+ IL  L H +LV LYGCT+R SRELLLVYE+I NGTLA+HLHGN+
Sbjct: 429 FERSLKRVEQFKNEIDILKSLKHPNLVILYGCTTRHSRELLLVYEYISNGTLAEHLHGNQ 488

Query: 727 AKSGELPWNTRLKIAIETASALAYLHASKTIHRDVKTTNILLDKNFCVKVADLGLSCLSP 786
           A+S  + W  RL+IAIETASAL+YLHAS  IHRDVKTTNILLD N+ VKVAD GLS L P
Sbjct: 489 AQSRPICWPARLQIAIETASALSYLHASGIIHRDVKTTNILLDSNYQVKVADFGLSRLFP 548

Query: 787 TQATHVSTAPQGTPGYVDPEYQECYQLTNKSDVFSFGVVLVELISSKPAVDVTRHRHEIN 846
              TH+STAPQGTPGYVDPEY +CY+L  KSDV+SFGVVL ELISSK AVD+TRHRH+IN
Sbjct: 549 MDQTHISTAPQGTPGYVDPEYYQCYRLNEKSDVYSFGVVLSELISSKEAVDITRHRHDIN 608

Query: 847 LWTMAINKIQNNDLHNFVDPSLRFETDQSVRDMIYAAAELAFRCLQSVKDMRPSMLEALE 884
           L  MAI+KIQN+ +H   D SL F  D SV+ M+ + AELAFRCLQ  +D+RPSM E +E
Sbjct: 609 LANMAISKIQNDAVHELADLSLGFARDPSVKKMMSSVAELAFRCLQQERDVRPSMDEIVE 634

BLAST of MS021656 vs. TAIR 10
Match: AT1G25390.1 (Protein kinase superfamily protein )

HSP 1 Score: 414.5 bits (1064), Expect = 2.2e-115
Identity = 212/348 (60.92%), Postives = 262/348 (75.29%), Query Frame = 0

Query: 538 VGCGIGGIILMGTISLILLRLQKNKHAHASSFPLGNNYFDPPSKELEKGENYMGVPLFSY 597
           +G GIGG +++  I + L  +    +       L     D    ++E  + +  +P+FSY
Sbjct: 225 LGLGIGGSVILIIILVALFAVIHRNYRRKDGSELSR---DNSKSDVEFSQVFFKIPIFSY 284

Query: 598 GELEEATDKFNPAKELGDGGFGTVYYGKLRDGREVAVKRLFENNYRKVEHFMNEVKILTC 657
            EL+ ATD F+  + LGDGGFGTVYYGK+RDGREVAVKRL+E+NYR++E FMNE++ILT 
Sbjct: 285 KELQAATDNFSKDRLLGDGGFGTVYYGKVRDGREVAVKRLYEHNYRRLEQFMNEIEILTR 344

Query: 658 LHHQHLVTLYGCTSRRSRELLLVYEFIPNGTLADHLHG-NRAKSGELPWNTRLKIAIETA 717
           LHH++LV+LYGCTSRRSRELLLVYEFIPNGT+ADHL+G N    G L W+ RL IAIETA
Sbjct: 345 LHHKNLVSLYGCTSRRSRELLLVYEFIPNGTVADHLYGENTPHQGFLTWSMRLSIAIETA 404

Query: 718 SALAYLHASKTIHRDVKTTNILLDKNFCVKVADLGLSCLSPTQATHVSTAPQGTPGYVDP 777
           SALAYLHAS  IHRDVKTTNILLD+NF VKVAD GLS L P+  THVSTAPQGTPGYVDP
Sbjct: 405 SALAYLHASDIIHRDVKTTNILLDRNFGVKVADFGLSRLLPSDVTHVSTAPQGTPGYVDP 464

Query: 778 EYQECYQLTNKSDVFSFGVVLVELISSKPAVDVTRHRHEINLWTMAINKIQNNDLHNFVD 837
           EY  CY LT+KSDV+SFGVVLVELISSKPAVD++R + EINL ++AINKIQN+  H  +D
Sbjct: 465 EYHRCYHLTDKSDVYSFGVVLVELISSKPAVDISRCKSEINLSSLAINKIQNHATHELID 524

Query: 838 PSLRFETDQSVRDMIYAAAELAFRCLQSVKDMRPSMLEALEILKNIES 885
            +L + T++ VR M    AELAF+CLQ    MRP+M + +  LK I++
Sbjct: 525 QNLGYATNEGVRKMTTMVAELAFQCLQQDNTMRPTMEQVVHELKGIQN 569