MS021649 (gene) Bitter gourd (TR) v1

Overview


Name	MS021649
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	AMSH-like ubiquitin thioesterase 1
Location	scaffold348: 59991 .. 65689 (+)
RNA-Seq Expression	MS021649
Synteny	MS021649

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGATCATCTTCGGAGAGAATTAACATCGCCGCCAGTACCCGGAGGCTCGATGTTGACAATCGATTTCCTCTTCGTTATTACTATCGAGTCGCCGATAATATTTTGAAACAGGTGACTCCGCCTCTTGAATTTCGCGTTCTAATTTTGCCCACCAACGTGAAATTGAGATTTTTTTGACGAGCGTTGAATTTGGGAGGGCTTATATATGCATATTCTTTCCTTTTATAAATAATAGATTATCTCGGGAATTCGATCTTGTAGTTTTCTCGCATGGATTATAGCTGCATTAAGTCGAGTAACGCAACGGGGGCTGTTGGAAATATTTTTGTAACAGATTATCACAAAAGATTGACGAAGTGTCCAGGAAACCAATCGATATTCTTTAGACTGGGCGTCAACAAATATACCAATGTTCTTCACAGTCAATAAACAAAAAACTACGATATATGTAGAAAATTTTATGGTTATGCGGGACGCTATAAAAGTGTATTGGACGAGTCTCTAAGCAGATTGAAGTTATGCGTGCAAAGCTTCGCTAACTTTTGATTTGAAAAAGGGCATATTTGTTTTTAAGTTAGAAATGGGTGTTTAGCAGGGGGGAGCTGGTTTGCTTTTAAGTCGTTTAATCGAATGTGTAGTTTTCTCGCCTGTTAGACTTCACGTTCCTCACTGCCTAACACAATTTACATTTCAGGCGGACATCTTTCGAGCAGAGAAGAACATAATTGATCTTTATGTCATGTTAATGAGATTTTCCAGGTAAGAACCTAAAATTAATGTCATCGAGTTCTAAAGAAACTATAATTTAACAGTTAAGTGCACTTGTGTAATTGAGATTTATGTCTTCTGTATTTGTACTTGGTATTTTTAAAAATTAACAGAATTGATTCAGCCAGTATGTTTGAGTTAGTTTGTAACTAGCTTCTTAATCATTATGTAGTCCTATAAGTATGTTAGTACCTGATCTACTTTTCAGTTCAAGAAATGAAAGATTAATTGATATCCATTATCTGTCTTTTTTGTTTGTGGGTATGTAGTTTGTGATTTATATATTGAAATTTACGGAGGCCGTCTTTCTTTAACCGCCATTCAGATTAGATAACGTTAGGTGTTCCATTCAAGATCAAGAATAACCCTAAATACATTTGGTTTTTTCATGCTTCAGTTTGGTCACCGAGACTATACCTCGCCATCGGGAGCATGGAACAACTCCTAAATACCAAAAAAACTATTTTAGAAAGGTAAATGACGCCTTTGGTGCAACTAATAAGCATTAAATTTGTGTTGCTGCATTTTATCCACTTTGTTTGTTCGGCAGAGGCTATTGAACGCTTTGAGCGAGCTTGAAGAATTGAAGCCAGCAGTTCAAAGAAAGGTGGATGAAATTAATGGAAAACAAAAACATCAAGTTAATGGGCGGGGTAATCAGCATCACAATGGTTCACGTTCATTGGAATGGGATTCTGGTGGAAAGCAATGGCTTACTTATAATGGTACAGCTAAGGTATAGTCGTGTTGTTACATAAATTTACTTTTCCTTTCAAGAACCTGACCATTGCCATTTTTAACACAACTGTTATTTATACTCCATGAAAAAATATAAGAAATTTGGTTTCTATATGCTGTCTTTTCTCTGACAGGATGGTAGATCAGCTGCTAGAGAAATTGCTCGTCAGGGTTCAGGGCCTCAACAATTTTCATATTCCAGGTCCGTGGATGAGAAGTTCTCCAGTTTGTGCATGTCTATAATCTAGATGTTTTCAAATTCTTCATTATAATATTGAACAAGCTTACAATAGATAGCATCCGTTATTTTAAGCATGCAAGTATGGCAGAATTATTTATGCTTTTTTTGGAAGCTACATTAAGCTCACAGTTCTAGTCTTGTTAACGAATATGGGTGATGTTGATACTATACGAATCTTTGTTGTCCTCATTCCTCATGCTTGTATCCTTGTAGACCTATTCATATCCCACTTCCCAAGGAGGAAACATTATCCCGTCATTCTATTTTGGGTCCCAATGGGCTTCATGGCCAGTGGCAACCACCTATCGTTGATAAAGGGGTATTTTCATACTTGGTAATTTTTTTTTGTGGTTTCTTTAGAAACACACTGATAGACATTGTCAATGTAGGTTCAATATCCACGTATTTTGGATCTGTCTCCTATTGAAATTCCAAGGTTTGTCTTCTAATTGAGTTTCTAAAAGGTCCAACTATTAGTTACCTGCAACTACATGTAATCCTTACAATGTGCTCAAGTCATCACCCATTAGGGCTACATGTTCAATCAAGTTCTTGCAGTTTCCAGTACTGCCTCCATGCTTTTTATTATGAAGATTTTATTGCCATGATATCTTTATTTCCTAAAGAAGGAGTTTTCCTCTACTGATTTGTTTGTAAACTTTTTTTCGCCCCATTTCCCACATAAACAGCTTACAACAATCAAAAGAAACCAAACTTACAATAGAAAAAGATCTTGGAAATGTGGAAAAAGAACGATCTGATACAGATTCAATATGCACCCAGAATGAGGATCCTCAGATACATAATGCTATGGAAAGAGGTTCCTTGATCTCCTTTGAAAAAGTTGAAATGCCTGCCCCTATTGAAATTGTGAGACAGCCTTCTCCTCCACCTGTACTTGCTGAAGTACAGGATCTGATACCTGCAATGTCACCTCAAGTTCCTGAGGTAGAATGTGCACGAGACATTTCATTGTCAGATGGTTTTGTTCATCCAGAGGCTTCTATGCAATTGCACATCGTGAGTTTTCTGACCTTTGTTTGTTGGTTCACTTTATATGCCTTCTATTTTCCAGTTCTTTCAGGAATAGCAGGCAAGTCCTGAAAAGAACCAGCCATAAAGATAGCAAGTCTTATCTGTTATTTGTTAGATTATGTGCATATGAATACATCATCTTTTGCTGAATATTTGGAAAATTTAGGGGCATTTTGCCTGGATGGACGTTTTAGATTGTGTTTCATTCTAGCAATTTCGCCTTTTGCAGTCAACCACGATGATGGACAGCTTCATGAGGCTAGCCAAGTCAAATACTGCCAAGAATTTAGAAACTTGTGGTGTTCTTGCTGGTTCACTTGTATGTTTTACTATTTTTCACCCTGCATATTTTTACAATTCTTATCATTATTGGCTGTTCATTTGCAACTCTTGATCAAAGTTCAACTAATGACTCTTTTTGCAGAAAAACAGAAAATTTTATGTTACTGCACTCATTGTACCAAAGCAGGAATCGACATCTGATACTGTATGTCTATGTGATGTCTGTTGTTAGATACTAGTTAATTTACATTTCACTCATCCAAGGAGTATAAATTATTAAAATATTAATCATCTGATCATCAGTGTCAGACGACCAATGAGGAAGAGATATTTGAAGTGCAGGATAAGCGTTCTTTATTCCCACTTGGGTGGATTCATGTAAGAATTCTGGTTTCATATTGTAGTTTATTGTATTATCATCACTTGGTACTTATTTAGTAGCACGTCTTTTAGGTCTTCTTCCGCAGTATTTTTTATGAACTCTTCGTTGGATTGAAATTCATTTCCAAGTTCCAAACTATAGACTTATTTCCTTTCAAACATGGCACTTTCTTAAAATAGGCTGTAAACCATGATAACAACTGTTGTAAAAACAACACTTCTATATATTATTCTTATCGGTTGAATAGGAGAGAGAAAAGGATTCAACCCTTGAAACCTAGAAAGTACATTCTGCATTCCAATAGTCCAAATGCTTCTCATTCTATTTTCTGCGAGAACATAGAATCTAGGATAATAGAGGCACAAAAGGAGACTCGTCTATCCAATCATCTTCCCAAAATCTAATAGAAGCTCAATGTGAGGTATATCCTCACTGTGAACTTCATTATTTGATTGAAAACAAAGAGAGCCTGTAAGCTTTAGACAGCAAACCCACCAATTCGAGAGGGTGGGGAATCTCTCCTACCTTCCTTCTATGCTCTAGGCACTGAATTCTAGACTACCCTTCCTAACCAATCCTCACTCATATTTATTCTCACACACACATGGCTAAGGCCTATTATTTCTCTCTCCTGGCCATTATGGCCCTAATTTCCCTTCTTGCCCTTCCTGCTATACGTCATCACGATAGGGGGCCTAACACTCTATTCTTACCTTAAAAAAGGATTTCTCCATAATTTTTCCATGGAAATTTGAGATAGAAAACCAAGAATTCTTTTGATGTTGGTCTCCCCTTTGGGAGTTGATGATGTTTGATTTAGCGCAAAAGACAGGCAGAAAACTGACAAATGTTTTAGTATTATACATCAATTGCTACTGACATTCCACTCCTAGGAAAATCATTTTAGGTGCAATAAAGATTTGGTGTTTCAAACTCAAATCATGTTCTGTTAAGGAATATCCAATTAGACTGCTCGTACATGACTGAGTGCCTGTTTGTTTTGTTTGTTTCGGACATGTATATCGATTTTCAATGCTAATGTTTCTTAATCTGTTTTCAACGTCTTAATAGTTGATAATATGTGTATGAATTGTGATATTTATGGCTATTATATATATATATATATATCTTATTTCATTGTTCCTATTGTGTAATTTCCAGACGCATCCTTCACAGTCATGCTTCATGTCGTCGGTTGATGTGCACACACATTACTCATATCAGGTATCTCATTTTCTGCAAAACATAAGTTAACATGTTGGATGTGGAGGAATACTTCACAAACTGAAACTTTTCTTCCCTGTTGCATGAACTATCACTATTATAGTTTGTAAGAAATGGAATTGGGTTTGCTATGGAACGTTATAGCTCCCGGTCAACCTTTCCAACTATTGTTTTAACTTTCTTTGTCACTCTAGACACAGATGAGGGGTAGAGAATTTTTTCTTTTAGAGGGAAGTTCGGTCCCTATTCCCCTGCCCATGCCCTCATGCTCTCTCAGGAAATTTTCTCAAGTTCCCTTGCCCGTTATCTTGTTTGTCTTATCTGGGGTAACTTTTCCTCTGGTGAGGGGAAAAAGGGAAGAGGAATGGGTGATACTCCGACTTGAATCAGGAGATATTTGAGGGAAGAGAGAGGAGGACTAGGAGGGGCGGGGTCATTGTGGAAAAGGATGTTTCTAAGGAGGAGATAGACTTGGCGCAGGATGAACAAGAAAATAAAAATTTGCATATGCAAAGTCGCTAGAAACTTAAAGGAGTGAGGGTACTGTTTGGTTCTCGCAAATACTTGGACCTTACAGTACAGTTATTTTAAAATCATATGAAACTATACAAATTTGTTTCTTCATTTCTTCCTATCTTTTAAGGTTTTAAGCTGTTGCTCATGAAGCTAATGTAGACCTTAATTTGCAGGTTATGTTGCCCGAAGCTGTTGCTATTGTGATGGCTCCCAAAGATAGTGCAAGGTATGATCATAAACCAAACTCATCGCTTAGATAGGATTTCATTAATAGTTACGATTATGTTGGAACAGTCTTAACATATTCTTTCTAAGAATATCCACCCTGATATGAACAGAACCCATGGCATTTTCCGGTTGACAACCCCGGGTGGCATGTCAGTTATTCGGCAATGCCAACAACGTGGCTTTCACCCGCATGGTCAGCCTCCTGATGGCGGACCCATTTACAAAACCTGTACAGATGTATACATGGACCCCAATTTGAAATTTGATGTCATCGATCTTCGG

mRNA sequence

ATGAGATCATCTTCGGAGAGAATTAACATCGCCGCCAGTACCCGGAGGCTCGATGTTGACAATCGATTTCCTCTTCGTTATTACTATCGAGTCGCCGATAATATTTTGAAACAGGCGGACATCTTTCGAGCAGAGAAGAACATAATTGATCTTTATGTCATGTTAATGAGATTTTCCAGTTTGGTCACCGAGACTATACCTCGCCATCGGGAGCATGGAACAACTCCTAAATACCAAAAAAACTATTTTAGAAAGAGGCTATTGAACGCTTTGAGCGAGCTTGAAGAATTGAAGCCAGCAGTTCAAAGAAAGGTGGATGAAATTAATGGAAAACAAAAACATCAAGTTAATGGGCGGGGTAATCAGCATCACAATGGTTCACGTTCATTGGAATGGGATTCTGGTGGAAAGCAATGGCTTACTTATAATGGTACAGCTAAGGATGGTAGATCAGCTGCTAGAGAAATTGCTCGTCAGGGTTCAGGGCCTCAACAATTTTCATATTCCAGACCTATTCATATCCCACTTCCCAAGGAGGAAACATTATCCCGTCATTCTATTTTGGGTCCCAATGGGCTTCATGGCCAGTGGCAACCACCTATCGTTGATAAAGGGGTTCAATATCCACGTATTTTGGATCTGTCTCCTATTGAAATTCCAAGCTTACAACAATCAAAAGAAACCAAACTTACAATAGAAAAAGATCTTGGAAATGTGGAAAAAGAACGATCTGATACAGATTCAATATGCACCCAGAATGAGGATCCTCAGATACATAATGCTATGGAAAGAGGTTCCTTGATCTCCTTTGAAAAAGTTGAAATGCCTGCCCCTATTGAAATTGTGAGACAGCCTTCTCCTCCACCTGTACTTGCTGAAGTACAGGATCTGATACCTGCAATGTCACCTCAAGTTCCTGAGGTAGAATGTGCACGAGACATTTCATTGTCAGATGGTTTTGTTCATCCAGAGGCTTCTATGCAATTGCACATCTCAACCACGATGATGGACAGCTTCATGAGGCTAGCCAAGTCAAATACTGCCAAGAATTTAGAAACTTGTGGTGTTCTTGCTGGTTCACTTAAAAACAGAAAATTTTATGTTACTGCACTCATTGTACCAAAGCAGGAATCGACATCTGATACTTGTCAGACGACCAATGAGGAAGAGATATTTGAAGTGCAGGATAAGCGTTCTTTATTCCCACTTGGGTGGATTCATACGCATCCTTCACAGTCATGCTTCATGTCGTCGGTTGATGTGCACACACATTACTCATATCAGGTTATGTTGCCCGAAGCTGTTGCTATTGTGATGGCTCCCAAAGATAGTGCAAGAACCCATGGCATTTTCCGGTTGACAACCCCGGGTGGCATGTCAGTTATTCGGCAATGCCAACAACGTGGCTTTCACCCGCATGGTCAGCCTCCTGATGGCGGACCCATTTACAAAACCTGTACAGATGTATACATGGACCCCAATTTGAAATTTGATGTCATCGATCTTCGG

Coding sequence (CDS)

ATGAGATCATCTTCGGAGAGAATTAACATCGCCGCCAGTACCCGGAGGCTCGATGTTGACAATCGATTTCCTCTTCGTTATTACTATCGAGTCGCCGATAATATTTTGAAACAGGCGGACATCTTTCGAGCAGAGAAGAACATAATTGATCTTTATGTCATGTTAATGAGATTTTCCAGTTTGGTCACCGAGACTATACCTCGCCATCGGGAGCATGGAACAACTCCTAAATACCAAAAAAACTATTTTAGAAAGAGGCTATTGAACGCTTTGAGCGAGCTTGAAGAATTGAAGCCAGCAGTTCAAAGAAAGGTGGATGAAATTAATGGAAAACAAAAACATCAAGTTAATGGGCGGGGTAATCAGCATCACAATGGTTCACGTTCATTGGAATGGGATTCTGGTGGAAAGCAATGGCTTACTTATAATGGTACAGCTAAGGATGGTAGATCAGCTGCTAGAGAAATTGCTCGTCAGGGTTCAGGGCCTCAACAATTTTCATATTCCAGACCTATTCATATCCCACTTCCCAAGGAGGAAACATTATCCCGTCATTCTATTTTGGGTCCCAATGGGCTTCATGGCCAGTGGCAACCACCTATCGTTGATAAAGGGGTTCAATATCCACGTATTTTGGATCTGTCTCCTATTGAAATTCCAAGCTTACAACAATCAAAAGAAACCAAACTTACAATAGAAAAAGATCTTGGAAATGTGGAAAAAGAACGATCTGATACAGATTCAATATGCACCCAGAATGAGGATCCTCAGATACATAATGCTATGGAAAGAGGTTCCTTGATCTCCTTTGAAAAAGTTGAAATGCCTGCCCCTATTGAAATTGTGAGACAGCCTTCTCCTCCACCTGTACTTGCTGAAGTACAGGATCTGATACCTGCAATGTCACCTCAAGTTCCTGAGGTAGAATGTGCACGAGACATTTCATTGTCAGATGGTTTTGTTCATCCAGAGGCTTCTATGCAATTGCACATCTCAACCACGATGATGGACAGCTTCATGAGGCTAGCCAAGTCAAATACTGCCAAGAATTTAGAAACTTGTGGTGTTCTTGCTGGTTCACTTAAAAACAGAAAATTTTATGTTACTGCACTCATTGTACCAAAGCAGGAATCGACATCTGATACTTGTCAGACGACCAATGAGGAAGAGATATTTGAAGTGCAGGATAAGCGTTCTTTATTCCCACTTGGGTGGATTCATACGCATCCTTCACAGTCATGCTTCATGTCGTCGGTTGATGTGCACACACATTACTCATATCAGGTTATGTTGCCCGAAGCTGTTGCTATTGTGATGGCTCCCAAAGATAGTGCAAGAACCCATGGCATTTTCCGGTTGACAACCCCGGGTGGCATGTCAGTTATTCGGCAATGCCAACAACGTGGCTTTCACCCGCATGGTCAGCCTCCTGATGGCGGACCCATTTACAAAACCTGTACAGATGTATACATGGACCCCAATTTGAAATTTGATGTCATCGATCTTCGG

Protein sequence

MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSSLVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRGNQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEETLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVEKERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPAMSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGSLKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVDVHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGGPIYKTCTDVYMDPNLKFDVIDLR

Homology

[Close]

BLAST of MS021649 vs. NCBI nr
Match: XP_022137393.1 (AMSH-like ubiquitin thioesterase 1 [Momordica charantia])

HSP 1 Score: 1003.0 bits (2592), Expect = 8.8e-289
Identity = 500/503 (99.40%), Postives = 500/503 (99.40%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS
Sbjct: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG
Sbjct: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180
           NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE
Sbjct: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180

Query: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240
           TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE
Sbjct: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240

Query: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300
           KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEM APIEIVRQPSPPPVLAEVQDLIPA
Sbjct: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMHAPIEIVRQPSPPPVLAEVQDLIPA 300

Query: 301 MSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGS 360
           MSPQVPEVECAR ISLSDGFVHPEASMQLHISTTMMD FMRLAKSNTAKNLETCGVLAGS
Sbjct: 301 MSPQVPEVECARGISLSDGFVHPEASMQLHISTTMMDGFMRLAKSNTAKNLETCGVLAGS 360

Query: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420
           LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD
Sbjct: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420

Query: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480
           VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG
Sbjct: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480

Query: 481 PIYKTCTDVYMDPNLKFDVIDLR 504
           PIYKTCTDVYMDPNLKFDVIDLR
Sbjct: 481 PIYKTCTDVYMDPNLKFDVIDLR 503

[Close]

BLAST of MS021649 vs. NCBI nr
Match: XP_022994179.1 (AMSH-like ubiquitin thioesterase 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 899.4 bits (2323), Expect = 1.4e-257
Identity = 453/503 (90.06%), Postives = 471/503 (93.64%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           MRSSSERINIAA TRRL+VDNRFPLRYYYR+ADNILKQADIFRAEKNIIDLYVMLMRFSS
Sbjct: 1   MRSSSERINIAACTRRLNVDNRFPLRYYYRIADNILKQADIFRAEKNIIDLYVMLMRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           L+TETIPRH E+GTT K QKNYFRKRLL+ALSELEELKPAVQRKVDEINGKQ++QVNG G
Sbjct: 61  LITETIPRHWEYGTTLKSQKNYFRKRLLDALSELEELKPAVQRKVDEINGKQRYQVNGWG 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180
           NQ  NG  SL W SGGKQ LT NG AK GRSAAREI RQGSGPQQFSYSR IHIPLPKEE
Sbjct: 121 NQQQNG--SLGWQSGGKQHLTDNGAAKAGRSAAREITRQGSGPQQFSYSRSIHIPLPKEE 180

Query: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240
           TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSP+EIPSLQQS ETKLT+ KDLGN+E
Sbjct: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPVEIPSLQQSIETKLTMGKDLGNLE 240

Query: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300
           K+RSD D ICTQNED ++HN ME  SLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA
Sbjct: 241 KDRSDIDPICTQNEDRKVHNTMETDSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300

Query: 301 MSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGS 360
           MSPQVPEVEC RD SLSD FVHPEAS+QLHISTTMM++FMRLAKSNTAKNLETCGVLAGS
Sbjct: 301 MSPQVPEVECTRDTSLSDKFVHPEASLQLHISTTMMENFMRLAKSNTAKNLETCGVLAGS 360

Query: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420
           LKNR FYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHP+QSCFMSSVD
Sbjct: 361 LKNRTFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPTQSCFMSSVD 420

Query: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480
           VHTHYSYQVMLPEAVAIVMAPKDSAR HGIFRLTTPGGMS+IRQCQQRGFH H QPPDGG
Sbjct: 421 VHTHYSYQVMLPEAVAIVMAPKDSARNHGIFRLTTPGGMSIIRQCQQRGFHSHNQPPDGG 480

Query: 481 PIYKTCTDVYMDPNLKFDVIDLR 504
           PIYKTCTDVYMDPNLKFDVIDLR
Sbjct: 481 PIYKTCTDVYMDPNLKFDVIDLR 501

[Close]

BLAST of MS021649 vs. NCBI nr
Match: XP_022924125.1 (AMSH-like ubiquitin thioesterase 1 [Cucurbita moschata])

HSP 1 Score: 897.1 bits (2317), Expect = 6.8e-257
Identity = 450/503 (89.46%), Postives = 476/503 (94.63%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           MRSSSERINIAASTRRL+VDNRFPLRYYYRVADN+LKQADIFRAEKNIIDLYVMLMRFSS
Sbjct: 1   MRSSSERINIAASTRRLNVDNRFPLRYYYRVADNVLKQADIFRAEKNIIDLYVMLMRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           LVTETIPRH E+GT+ K QKNYFRK+LLNALSELEELKP+VQRKVDEINGKQK+QVNGRG
Sbjct: 61  LVTETIPRHWEYGTSLKSQKNYFRKKLLNALSELEELKPSVQRKVDEINGKQKYQVNGRG 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180
           NQH NGS   +W SGGKQ L  NGTA+ GRSAA EIA QGSGPQQFSYSR IHIPLP EE
Sbjct: 121 NQHQNGSS--DWHSGGKQHLIDNGTARAGRSAATEIALQGSGPQQFSYSRSIHIPLPTEE 180

Query: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240
           TLSRHSILGPNGLHG+WQPPIVDKGVQYPRILDLSP+EIPSLQQS ETKLTIEKDLGN+E
Sbjct: 181 TLSRHSILGPNGLHGKWQPPIVDKGVQYPRILDLSPVEIPSLQQSIETKLTIEKDLGNLE 240

Query: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300
           KER D DSICT+++D +I+NAME  SLISFEKVEMPAP+EIVRQPSPPPVLAEVQDLIPA
Sbjct: 241 KERPDIDSICTEDKDCKINNAMETDSLISFEKVEMPAPVEIVRQPSPPPVLAEVQDLIPA 300

Query: 301 MSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGS 360
           MSPQVPEVECA+D SLSDGFVHPEASMQLHIST MM+SFMRLAKSNTAKNLETCG+LAGS
Sbjct: 301 MSPQVPEVECAQDTSLSDGFVHPEASMQLHISTKMMESFMRLAKSNTAKNLETCGILAGS 360

Query: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420
           LKNRKFY+TALIVPKQEST +TCQ TNEEEIF+VQDKRSLFPLGWIHTHPSQSCFMSSVD
Sbjct: 361 LKNRKFYITALIVPKQESTPNTCQATNEEEIFDVQDKRSLFPLGWIHTHPSQSCFMSSVD 420

Query: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480
           VHTHYSYQVMLPEAVAIVMAP+DSARTHGIFRLTTPGGMS+IRQCQQRGFH HGQPPDGG
Sbjct: 421 VHTHYSYQVMLPEAVAIVMAPQDSARTHGIFRLTTPGGMSIIRQCQQRGFHSHGQPPDGG 480

Query: 481 PIYKTCTDVYMDPNLKFDVIDLR 504
           PIYKTCTDVYMDPNLKFDVIDLR
Sbjct: 481 PIYKTCTDVYMDPNLKFDVIDLR 501

[Close]

BLAST of MS021649 vs. NCBI nr
Match: XP_022955249.1 (AMSH-like ubiquitin thioesterase 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 896.7 bits (2316), Expect = 8.9e-257
Identity = 451/503 (89.66%), Postives = 470/503 (93.44%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           MRSSSERINIAA TRRL+VDNRFPLRYYYR+ADNILKQADIFRAEKNIIDLYVMLMRFSS
Sbjct: 1   MRSSSERINIAACTRRLNVDNRFPLRYYYRIADNILKQADIFRAEKNIIDLYVMLMRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           L+TETIPRH E+G T   QKNYFRKRLL+ALSELEELKPAVQRKVDEINGKQ++QVNG G
Sbjct: 61  LITETIPRHWEYGRTLNSQKNYFRKRLLDALSELEELKPAVQRKVDEINGKQRYQVNGWG 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180
           NQ  NG  SL W SGGKQ+LT NG AK GRSAAREI RQGSGPQQFSYSR IHIPLPKEE
Sbjct: 121 NQQQNG--SLGWQSGGKQYLTDNGAAKAGRSAAREITRQGSGPQQFSYSRSIHIPLPKEE 180

Query: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240
           TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSP+EIPSLQQ+ ETKLTI KDLGN+E
Sbjct: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPVEIPSLQQAIETKLTIGKDLGNLE 240

Query: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300
           K+RSD D ICTQNED ++HN ME  SLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA
Sbjct: 241 KDRSDIDPICTQNEDRKVHNTMETDSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300

Query: 301 MSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGS 360
           MSPQVPEVEC RD SLSD FVHPEAS+QLHISTTMM++FMRLAKSNTAKNLETCGVLAGS
Sbjct: 301 MSPQVPEVECTRDTSLSDKFVHPEASLQLHISTTMMENFMRLAKSNTAKNLETCGVLAGS 360

Query: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420
           LKNR FYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHP+QSCFMSSVD
Sbjct: 361 LKNRTFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPTQSCFMSSVD 420

Query: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480
           VHTHYSYQVMLPEAVAIVMAPKDSAR HGIFRLTTPGGMS+IRQCQQRGFH H QPPDGG
Sbjct: 421 VHTHYSYQVMLPEAVAIVMAPKDSARNHGIFRLTTPGGMSIIRQCQQRGFHSHNQPPDGG 480

Query: 481 PIYKTCTDVYMDPNLKFDVIDLR 504
           PIYKTCTDVYMDPNLKFDVIDLR
Sbjct: 481 PIYKTCTDVYMDPNLKFDVIDLR 501

[Close]

BLAST of MS021649 vs. NCBI nr
Match: KAG6584355.1 (AMSH-like ubiquitin thioesterase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 896.3 bits (2315), Expect = 1.2e-256
Identity = 448/503 (89.07%), Postives = 477/503 (94.83%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           MRSSSERINIAASTRRL+VDNRFPLRYYYRVADN+LKQADIFRAEKNIIDLYVMLMRFSS
Sbjct: 1   MRSSSERINIAASTRRLNVDNRFPLRYYYRVADNVLKQADIFRAEKNIIDLYVMLMRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           LVTETIPRH E+GT+ K QKNYFRK+LLNALSELEELKP+VQRKVDEINGKQK+QVNGRG
Sbjct: 61  LVTETIPRHWEYGTSLKSQKNYFRKKLLNALSELEELKPSVQRKVDEINGKQKYQVNGRG 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180
           NQH NGS   +W SGGKQ+L  NGTA+ GRSAA EIA QGSGPQQFSYSR IHIPLP EE
Sbjct: 121 NQHQNGSS--DWHSGGKQYLIDNGTARAGRSAATEIALQGSGPQQFSYSRSIHIPLPTEE 180

Query: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240
           TLSRHSILGPNGLHG+WQPPIVDKGVQYPRILDLSP+EIPSLQQS ETKLTIEKDLGN+E
Sbjct: 181 TLSRHSILGPNGLHGKWQPPIVDKGVQYPRILDLSPVEIPSLQQSIETKLTIEKDLGNLE 240

Query: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300
           KER D D+ICT+++D +I+NAME  SLISFEKVEMPAP+EIVRQPSPPPVLAEVQDLIPA
Sbjct: 241 KERPDIDTICTEDKDCKINNAMETDSLISFEKVEMPAPVEIVRQPSPPPVLAEVQDLIPA 300

Query: 301 MSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGS 360
           +SPQVPEVECA+D SLSDGFVHPEASMQLHIST MM+SFMRLAKSNTAKNLETCG+LAGS
Sbjct: 301 LSPQVPEVECAQDTSLSDGFVHPEASMQLHISTKMMESFMRLAKSNTAKNLETCGILAGS 360

Query: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420
           LKNRKFY+TALIVPKQEST +TCQ TNEEEIF+VQDKRSLFPLGWIHTHPSQSCFMSSVD
Sbjct: 361 LKNRKFYITALIVPKQESTPNTCQATNEEEIFDVQDKRSLFPLGWIHTHPSQSCFMSSVD 420

Query: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480
           VHTHYSYQVMLPEAVAIVMAP+DSARTHGIFRLTTPGGMS+IRQCQQRGFH HGQPPDGG
Sbjct: 421 VHTHYSYQVMLPEAVAIVMAPQDSARTHGIFRLTTPGGMSIIRQCQQRGFHSHGQPPDGG 480

Query: 481 PIYKTCTDVYMDPNLKFDVIDLR 504
           PIYKTCTDVYMDPNLKFDVIDLR
Sbjct: 481 PIYKTCTDVYMDPNLKFDVIDLR 501

[Close]

BLAST of MS021649 vs. ExPASy Swiss-Prot
Match: Q8VYB5 (AMSH-like ubiquitin thioesterase 1 OS=Arabidopsis thaliana OX=3702 GN=AMSH1 PE=2 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 4.9e-165
Identity = 307/511 (60.08%), Postives = 367/511 (71.82%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           M SS E I+IA S RR+ VDNR  L++Y+R+ADNILKQA+IFRAEKN+IDLYVML+RFSS
Sbjct: 1   MGSSFETIDIATSARRIGVDNRISLKFYFRIADNILKQANIFRAEKNVIDLYVMLLRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           L  ETIP HR++ T+ K  K Y R RLL+ L+ELE+LKP VQ+++DE+  K K + N   
Sbjct: 61  LALETIPSHRDYRTSLKSNKEYLRMRLLDVLTELEKLKPVVQQRIDELYPKLKPRYN--- 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPL---- 180
            Q H  + SL W S  K        AK            GS  QQF  + P+        
Sbjct: 121 VQAHPANGSLGWSSAVKPSFNSYDHAKVRNPPGHNSGYMGSRGQQFLNAAPLEERFRKMS 180

Query: 181 ----PKEETLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTI 240
               P EETLS+HSILGP GL  QWQPP  D  VQYP  +D SP+ IPS QQ  ++K  I
Sbjct: 181 VNFRPNEETLSKHSILGPGGLSAQWQPPKYDTKVQYPSNIDFSPVVIPSFQQLVDSKPMI 240

Query: 241 EKDLGNVEKERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLA 300
                N E E+   +     NE  Q +   E  S+ISFE+ E      ++RQPSPPPVLA
Sbjct: 241 TNG-SNDEPEKPIVEPSVASNEKIQKNYTEELSSMISFEEPESVNENNLIRQPSPPPVLA 300

Query: 301 EVQDLIPAMSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLE 360
           EVQDL+PA+ P+V E EC  + SL D  +  E+ ++LHI+T+MMD+FMRLAKSNT KNLE
Sbjct: 301 EVQDLVPALCPEVREPECMIENSLPDESLRSESPLELHIATSMMDTFMRLAKSNTKKNLE 360

Query: 361 TCGVLAGSLKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQ 420
           TCG+LAGSLKNRKFY+TALI+PKQESTSD+CQ TNEEEIFEVQDK+SLFPLGWIHTHP+Q
Sbjct: 361 TCGILAGSLKNRKFYITALIIPKQESTSDSCQATNEEEIFEVQDKQSLFPLGWIHTHPTQ 420

Query: 421 SCFMSSVDVHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHP 480
           SCFMSS+DVHTHYSYQ+MLPEAVAIVMAP+DS+R HGIFRLTTPGGM+VIR C +RGFH 
Sbjct: 421 SCFMSSIDVHTHYSYQIMLPEAVAIVMAPQDSSRNHGIFRLTTPGGMTVIRNCDRRGFHA 480

Query: 481 HGQPPDGGPIYKTCTDVYMDPNLKFDVIDLR 504
           H  P DGGPIY TC +VYM+PNLKFDVIDLR
Sbjct: 481 HSSPEDGGPIYNTCKEVYMNPNLKFDVIDLR 507

[Close]

BLAST of MS021649 vs. ExPASy Swiss-Prot
Match: Q5PNU3 (AMSH-like ubiquitin thioesterase 3 OS=Arabidopsis thaliana OX=3702 GN=AMSH3 PE=1 SV=2)

HSP 1 Score: 403.7 bits (1036), Expect = 3.1e-111
Identity = 232/522 (44.44%), Postives = 315/522 (60.34%), Query Frame = 0


Query: 7   RINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSSLVTETI 66
           +I++    R ++VDNR PLR YYR+ADN+L+QA I+R EKN++DLY+ML+R+SSL++ETI
Sbjct: 2   KIDLNKVAREIEVDNRIPLRNYYRIADNLLRQASIYREEKNVVDLYIMLLRYSSLISETI 61

Query: 67  PRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRGNQHHNG 126
           P HR++  +   ++   RKRL   ++ELE LKP   + VD++N     +V     Q  + 
Sbjct: 62  PFHRDYQASLPQERLGSRKRLRAVINELESLKPEFNQLVDKLN-----RVEDESRQDGSD 121

Query: 127 SRSLEWDSGGKQW-----------------------LTYNGTAKDGRSAAREIARQGSGP 186
              + + S   +W                        TYN       +  +         
Sbjct: 122 LPVVSYSSDAVEWPPAHKASYSRPDINKPLPTSQPSWTYNNNLTSSSNRTQI-------D 181

Query: 187 QQFSYSRPIHIPLPKEETLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQ 246
           QQF       +P P + TLSRHS LGPNGL  Q   P  +  VQYP   D    +   L 
Sbjct: 182 QQFQKLSFDFLP-PNQATLSRHSFLGPNGLKRQMVAPKSEIKVQYPSNTDWGSADNSGLI 241

Query: 247 QSKETKLTIEKDLGNVEKERSDTDSICTQNEDP-QIHNAMERGSLISFEKVEMPAPIEIV 306
           ++  +  +    L    +E S  +S+ + ++   Q H+       IS +  E P     +
Sbjct: 242 EAGPS--SSSASLNGDSQEVSTLNSVLSLDDGRWQRHSEAVNSQFIS-DATEDPFQFVGM 301

Query: 307 RQPSPPPVLAEV-QDLIPAMSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMR 366
           +QPSPPPVLA+V Q+L      +V +      I   +G     +   LH+   +MD F+R
Sbjct: 302 KQPSPPPVLAQVHQELAQICPSKVADPRPGPAIPSLEGKEGSNSYQHLHVPVRIMDDFLR 361

Query: 367 LAKSNTAKNLETCGVLAGSLKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLF 426
           LA+SNT +NLETCGVLAGSLKNR F++T LI+PKQESTSD+CQT NEEEIFEVQD+ SLF
Sbjct: 362 LARSNTERNLETCGVLAGSLKNRVFHITTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLF 421

Query: 427 PLGWIHTHPSQSCFMSSVDVHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSV 486
           PLGWIHTHP+Q+CFMSSVD+HTHYSYQ+MLPEAVAIVMAP D +  HGIF L+ P G+SV
Sbjct: 422 PLGWIHTHPTQTCFMSSVDLHTHYSYQIMLPEAVAIVMAPTDESTPHGIFHLSDPSGVSV 481

Query: 487 IRQCQQRGFHPHGQPPDGGPIYKTCTDVYMDPNLKFDVIDLR 504
           IR CQQRGFHPH +  DG PIY+ C+ V+++  LK++V+DLR
Sbjct: 482 IRNCQQRGFHPHEESEDGNPIYEHCSHVFLNAKLKYEVLDLR 507

[Close]

BLAST of MS021649 vs. ExPASy Swiss-Prot
Match: Q6NKP9 (AMSH-like ubiquitin thioesterase 2 OS=Arabidopsis thaliana OX=3702 GN=AMSH2 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.9e-64
Identity = 121/228 (53.07%), Postives = 163/228 (71.49%), Query Frame = 0


Query: 281 IVRQPSPPPVLAEVQDLIPAMSPQVPEVECARDISLSDGFVHPEAS-----MQLHISTTM 340
           +V   SP P L+ V++ +   S  V  V     IS +D   H E+S       +HIS  +
Sbjct: 1   MVTLSSPSPSLSCVEN-VTCKSSHVSRVL----ISGTDNINHGESSEAKILRDVHISERL 60

Query: 341 MDSFMRLAKSNTAKNLETCGVLAGSLKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQ 400
           ++ F  LA+ NT K+LETCG LA  L+   FYVT LI+PKQESTS++CQ  NE E+F +Q
Sbjct: 61  LEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQESTSNSCQAMNEVEVFSIQ 120

Query: 401 DKRSLFPLGWIHTHPSQSCFMSSVDVHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTT 460
           ++R L+P+GWIHTHPSQ CFMSSVD+HTHYSYQVM+PEA AIV+AP DS++++GIF+LT 
Sbjct: 121 NERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPTDSSKSYGIFKLTD 180

Query: 461 PGGMSVIRQCQQRGFHPHGQPPDGGPIYKTCTDVYMDPNLKFDVIDLR 504
           PGGM V+R C + GFHPH +P DG P+Y+ C++VY + NL+F++ DLR
Sbjct: 181 PGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDLR 223

[Close]

BLAST of MS021649 vs. ExPASy Swiss-Prot
Match: Q9P371 (AMSH-like protease sst2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sst2 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.5e-44
Identity = 91/178 (51.12%), Postives = 119/178 (66.85%), Query Frame = 0


Query: 329 LHISTTMMDSFMRLAKSNTAKNLETCGVLAGSLKNRKFYVTALIVPKQESTSDTCQTTNE 388
           +++   +   F+ + K NT KNLETCG+L G L+   F++T L++P QE+TSDTC TT+E
Sbjct: 263 IYLPKLLKKVFLDVVKPNTKKNLETCGILCGKLRQNAFFITHLVIPLQEATSDTCGTTDE 322

Query: 389 EEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVDVHTHYSYQVMLPEAVAIVMAPKDSARTH 448
             +FE QDK +L  LGWIHTHP+Q+CFMSSVD+HTH SYQ+MLPEA+AIVMAP  S  T 
Sbjct: 323 ASLFEFQDKHNLLTLGWIHTHPTQTCFMSSVDLHTHCSYQLMLPEAIAIVMAP--SKNTS 382

Query: 449 GIFRLTTPGGMSVIRQCQQRG-FHPHGQPPDGGPIYKTCTDV--YMDPNLKFDVIDLR 504
           GIFRL  P G+  I +C++ G FHPH      G +Y          + N K  V+DLR
Sbjct: 383 GIFRLLDPEGLQTIVKCRKPGLFHPH-----EGKVYTMVAQPGHVREINSKLQVVDLR 433

[Close]

BLAST of MS021649 vs. ExPASy Swiss-Prot
Match: Q54Q40 (Probable ubiquitin thioesterase DG1039 OS=Dictyostelium discoideum OX=44689 GN=DG1039 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.8e-43
Identity = 86/171 (50.29%), Postives = 118/171 (69.01%), Query Frame = 0


Query: 335 MMDSFMRLAKSNTAKNLETCGVLAGSLKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEV 394
           +   FMRLA++NT +++ETCG+L+G+L N  F +T +I+PKQE T+DTC T  E EIFE 
Sbjct: 543 VFQEFMRLAENNTKRSIETCGILSGTLSNDVFRITTIIIPKQEGTTDTCNTIEEHEIFEY 602

Query: 395 QDKRSLFPLGWIHTHPSQSCFMSSVDVHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLT 454
           Q +  L  LGWIHTHP+Q CF+S+VDVHTH SYQ +L EA+A+V++P  +    GIFRLT
Sbjct: 603 QLENDLLTLGWIHTHPTQDCFLSAVDVHTHCSYQYLLQEAIAVVISPMANP-NFGIFRLT 662

Query: 455 TPGGMSVIRQCQQRGFHPHGQPPDGGPIYKTCTDVYMDPNLKFD--VIDLR 504
            P G+  +++C+ + FHPH  P +G PIY     V +    K D  V+DLR
Sbjct: 663 DPPGLETVQKCKLKSFHPH-PPVNGIPIYTKVDHVDLIWGKKSDSKVVDLR 711

HSP 2 Score: 48.9 bits (115), Expect = 1.9e-04
Identity = 26/100 (26.00%), Postives = 55/100 (55.00%), Query Frame = 0


Query: 17  LDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSSLVTETIPRHREHGTTP 76
           ++VD  + + +Y    +N++KQADI+++E +I   Y+  +RF  L+ E + +H +     
Sbjct: 24  VEVDKNYSIFHYLSTCNNLVKQADIYKSEGDIERTYIYSLRFCILIFEKLQKHPDFNKES 83

Query: 77  -KYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQ 116
               +N  +++    L ELE LK  +++  + I  K++ +
Sbjct: 84  FTKSRNEIKRKAELKLKELEGLKETLKKGYERIQHKKEEE 123

[Close]

BLAST of MS021649 vs. ExPASy TrEMBL
Match: A0A6J1CA83 (AMSH-like ubiquitin thioesterase 1 OS=Momordica charantia OX=3673 GN=LOC111008855 PE=3 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.3e-289
Identity = 500/503 (99.40%), Postives = 500/503 (99.40%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS
Sbjct: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG
Sbjct: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180
           NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE
Sbjct: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180

Query: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240
           TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE
Sbjct: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240

Query: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300
           KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEM APIEIVRQPSPPPVLAEVQDLIPA
Sbjct: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMHAPIEIVRQPSPPPVLAEVQDLIPA 300

Query: 301 MSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGS 360
           MSPQVPEVECAR ISLSDGFVHPEASMQLHISTTMMD FMRLAKSNTAKNLETCGVLAGS
Sbjct: 301 MSPQVPEVECARGISLSDGFVHPEASMQLHISTTMMDGFMRLAKSNTAKNLETCGVLAGS 360

Query: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420
           LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD
Sbjct: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420

Query: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480
           VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG
Sbjct: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480

Query: 481 PIYKTCTDVYMDPNLKFDVIDLR 504
           PIYKTCTDVYMDPNLKFDVIDLR
Sbjct: 481 PIYKTCTDVYMDPNLKFDVIDLR 503

[Close]

BLAST of MS021649 vs. ExPASy TrEMBL
Match: A0A6J1K266 (AMSH-like ubiquitin thioesterase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489995 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 6.7e-258
Identity = 453/503 (90.06%), Postives = 471/503 (93.64%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           MRSSSERINIAA TRRL+VDNRFPLRYYYR+ADNILKQADIFRAEKNIIDLYVMLMRFSS
Sbjct: 1   MRSSSERINIAACTRRLNVDNRFPLRYYYRIADNILKQADIFRAEKNIIDLYVMLMRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           L+TETIPRH E+GTT K QKNYFRKRLL+ALSELEELKPAVQRKVDEINGKQ++QVNG G
Sbjct: 61  LITETIPRHWEYGTTLKSQKNYFRKRLLDALSELEELKPAVQRKVDEINGKQRYQVNGWG 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180
           NQ  NG  SL W SGGKQ LT NG AK GRSAAREI RQGSGPQQFSYSR IHIPLPKEE
Sbjct: 121 NQQQNG--SLGWQSGGKQHLTDNGAAKAGRSAAREITRQGSGPQQFSYSRSIHIPLPKEE 180

Query: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240
           TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSP+EIPSLQQS ETKLT+ KDLGN+E
Sbjct: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPVEIPSLQQSIETKLTMGKDLGNLE 240

Query: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300
           K+RSD D ICTQNED ++HN ME  SLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA
Sbjct: 241 KDRSDIDPICTQNEDRKVHNTMETDSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300

Query: 301 MSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGS 360
           MSPQVPEVEC RD SLSD FVHPEAS+QLHISTTMM++FMRLAKSNTAKNLETCGVLAGS
Sbjct: 301 MSPQVPEVECTRDTSLSDKFVHPEASLQLHISTTMMENFMRLAKSNTAKNLETCGVLAGS 360

Query: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420
           LKNR FYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHP+QSCFMSSVD
Sbjct: 361 LKNRTFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPTQSCFMSSVD 420

Query: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480
           VHTHYSYQVMLPEAVAIVMAPKDSAR HGIFRLTTPGGMS+IRQCQQRGFH H QPPDGG
Sbjct: 421 VHTHYSYQVMLPEAVAIVMAPKDSARNHGIFRLTTPGGMSIIRQCQQRGFHSHNQPPDGG 480

Query: 481 PIYKTCTDVYMDPNLKFDVIDLR 504
           PIYKTCTDVYMDPNLKFDVIDLR
Sbjct: 481 PIYKTCTDVYMDPNLKFDVIDLR 501

[Close]

BLAST of MS021649 vs. ExPASy TrEMBL
Match: A0A6J1EDX3 (AMSH-like ubiquitin thioesterase 1 OS=Cucurbita moschata OX=3662 GN=LOC111431654 PE=3 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.3e-257
Identity = 450/503 (89.46%), Postives = 476/503 (94.63%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           MRSSSERINIAASTRRL+VDNRFPLRYYYRVADN+LKQADIFRAEKNIIDLYVMLMRFSS
Sbjct: 1   MRSSSERINIAASTRRLNVDNRFPLRYYYRVADNVLKQADIFRAEKNIIDLYVMLMRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           LVTETIPRH E+GT+ K QKNYFRK+LLNALSELEELKP+VQRKVDEINGKQK+QVNGRG
Sbjct: 61  LVTETIPRHWEYGTSLKSQKNYFRKKLLNALSELEELKPSVQRKVDEINGKQKYQVNGRG 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180
           NQH NGS   +W SGGKQ L  NGTA+ GRSAA EIA QGSGPQQFSYSR IHIPLP EE
Sbjct: 121 NQHQNGSS--DWHSGGKQHLIDNGTARAGRSAATEIALQGSGPQQFSYSRSIHIPLPTEE 180

Query: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240
           TLSRHSILGPNGLHG+WQPPIVDKGVQYPRILDLSP+EIPSLQQS ETKLTIEKDLGN+E
Sbjct: 181 TLSRHSILGPNGLHGKWQPPIVDKGVQYPRILDLSPVEIPSLQQSIETKLTIEKDLGNLE 240

Query: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300
           KER D DSICT+++D +I+NAME  SLISFEKVEMPAP+EIVRQPSPPPVLAEVQDLIPA
Sbjct: 241 KERPDIDSICTEDKDCKINNAMETDSLISFEKVEMPAPVEIVRQPSPPPVLAEVQDLIPA 300

Query: 301 MSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGS 360
           MSPQVPEVECA+D SLSDGFVHPEASMQLHIST MM+SFMRLAKSNTAKNLETCG+LAGS
Sbjct: 301 MSPQVPEVECAQDTSLSDGFVHPEASMQLHISTKMMESFMRLAKSNTAKNLETCGILAGS 360

Query: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420
           LKNRKFY+TALIVPKQEST +TCQ TNEEEIF+VQDKRSLFPLGWIHTHPSQSCFMSSVD
Sbjct: 361 LKNRKFYITALIVPKQESTPNTCQATNEEEIFDVQDKRSLFPLGWIHTHPSQSCFMSSVD 420

Query: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480
           VHTHYSYQVMLPEAVAIVMAP+DSARTHGIFRLTTPGGMS+IRQCQQRGFH HGQPPDGG
Sbjct: 421 VHTHYSYQVMLPEAVAIVMAPQDSARTHGIFRLTTPGGMSIIRQCQQRGFHSHGQPPDGG 480

Query: 481 PIYKTCTDVYMDPNLKFDVIDLR 504
           PIYKTCTDVYMDPNLKFDVIDLR
Sbjct: 481 PIYKTCTDVYMDPNLKFDVIDLR 501

[Close]

BLAST of MS021649 vs. ExPASy TrEMBL
Match: A0A6J1GVL2 (AMSH-like ubiquitin thioesterase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457266 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.3e-257
Identity = 451/503 (89.66%), Postives = 470/503 (93.44%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           MRSSSERINIAA TRRL+VDNRFPLRYYYR+ADNILKQADIFRAEKNIIDLYVMLMRFSS
Sbjct: 1   MRSSSERINIAACTRRLNVDNRFPLRYYYRIADNILKQADIFRAEKNIIDLYVMLMRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           L+TETIPRH E+G T   QKNYFRKRLL+ALSELEELKPAVQRKVDEINGKQ++QVNG G
Sbjct: 61  LITETIPRHWEYGRTLNSQKNYFRKRLLDALSELEELKPAVQRKVDEINGKQRYQVNGWG 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180
           NQ  NG  SL W SGGKQ+LT NG AK GRSAAREI RQGSGPQQFSYSR IHIPLPKEE
Sbjct: 121 NQQQNG--SLGWQSGGKQYLTDNGAAKAGRSAAREITRQGSGPQQFSYSRSIHIPLPKEE 180

Query: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240
           TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSP+EIPSLQQ+ ETKLTI KDLGN+E
Sbjct: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPVEIPSLQQAIETKLTIGKDLGNLE 240

Query: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300
           K+RSD D ICTQNED ++HN ME  SLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA
Sbjct: 241 KDRSDIDPICTQNEDRKVHNTMETDSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300

Query: 301 MSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGS 360
           MSPQVPEVEC RD SLSD FVHPEAS+QLHISTTMM++FMRLAKSNTAKNLETCGVLAGS
Sbjct: 301 MSPQVPEVECTRDTSLSDKFVHPEASLQLHISTTMMENFMRLAKSNTAKNLETCGVLAGS 360

Query: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420
           LKNR FYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHP+QSCFMSSVD
Sbjct: 361 LKNRTFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPTQSCFMSSVD 420

Query: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480
           VHTHYSYQVMLPEAVAIVMAPKDSAR HGIFRLTTPGGMS+IRQCQQRGFH H QPPDGG
Sbjct: 421 VHTHYSYQVMLPEAVAIVMAPKDSARNHGIFRLTTPGGMSIIRQCQQRGFHSHNQPPDGG 480

Query: 481 PIYKTCTDVYMDPNLKFDVIDLR 504
           PIYKTCTDVYMDPNLKFDVIDLR
Sbjct: 481 PIYKTCTDVYMDPNLKFDVIDLR 501

[Close]

BLAST of MS021649 vs. ExPASy TrEMBL
Match: A0A6J1KK10 (AMSH-like ubiquitin thioesterase 1 OS=Cucurbita maxima OX=3661 GN=LOC111495308 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.6e-256
Identity = 449/503 (89.26%), Postives = 475/503 (94.43%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           MRSSSERINIAASTRRL+VDNRFPLRYYYRVADN+LKQADIFRAEKNIIDLYVMLMRFSS
Sbjct: 1   MRSSSERINIAASTRRLNVDNRFPLRYYYRVADNVLKQADIFRAEKNIIDLYVMLMRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           LVTETIPRH E+GT+ K QKNYFRK+LLNALSELEELKP+VQRKVDEINGKQK+Q NGRG
Sbjct: 61  LVTETIPRHWEYGTSLKSQKNYFRKKLLNALSELEELKPSVQRKVDEINGKQKYQGNGRG 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPLPKEE 180
           NQH NGS   +W SGGKQ+L  NGTA+ GRSAA EIA QGSGPQQFSYSR IHIPLP EE
Sbjct: 121 NQHQNGSS--DWHSGGKQYLIDNGTARAGRSAATEIALQGSGPQQFSYSRSIHIPLPTEE 180

Query: 181 TLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTIEKDLGNVE 240
           TLSRHSILGPNGLHG+WQPPIVDKGVQYPRILDLSP+EIPSLQ+S ETKLTIEKDLGN+E
Sbjct: 181 TLSRHSILGPNGLHGKWQPPIVDKGVQYPRILDLSPVEIPSLQESIETKLTIEKDLGNLE 240

Query: 241 KERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLAEVQDLIPA 300
           KER D DSICT++ D +I+NAME  SLISFEKVEMPAP+EIVRQPSPPPVLAEVQDLIPA
Sbjct: 241 KERPDIDSICTEDNDCKINNAMETDSLISFEKVEMPAPVEIVRQPSPPPVLAEVQDLIPA 300

Query: 301 MSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLETCGVLAGS 360
           MSPQVPEVECA+D SLSDGFVHPEASMQLHIST MM+SFMRLAKSNTAKNLETCGVLAGS
Sbjct: 301 MSPQVPEVECAQDTSLSDGFVHPEASMQLHISTKMMESFMRLAKSNTAKNLETCGVLAGS 360

Query: 361 LKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVD 420
           LKNRKFY+TALIVPKQEST +TCQ TNEEEIF+VQDKRSLFPLGWIHTHPSQSCFMSSVD
Sbjct: 361 LKNRKFYITALIVPKQESTPNTCQATNEEEIFDVQDKRSLFPLGWIHTHPSQSCFMSSVD 420

Query: 421 VHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGG 480
           VHTHYSYQVMLPEAVAIVMAP+DSARTHGIFRLTTPGGMS+IRQCQQRGFH HGQPPDGG
Sbjct: 421 VHTHYSYQVMLPEAVAIVMAPQDSARTHGIFRLTTPGGMSIIRQCQQRGFHSHGQPPDGG 480

Query: 481 PIYKTCTDVYMDPNLKFDVIDLR 504
           PIYKTCTDVYMDPNLKFDVIDLR
Sbjct: 481 PIYKTCTDVYMDPNLKFDVIDLR 501

[Close]

BLAST of MS021649 vs. TAIR 10
Match: AT1G48790.1 (associated molecule with the SH3 domain of STAM 1 )

HSP 1 Score: 582.4 bits (1500), Expect = 3.5e-166
Identity = 307/511 (60.08%), Postives = 367/511 (71.82%), Query Frame = 0


Query: 1   MRSSSERINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSS 60
           M SS E I+IA S RR+ VDNR  L++Y+R+ADNILKQA+IFRAEKN+IDLYVML+RFSS
Sbjct: 1   MGSSFETIDIATSARRIGVDNRISLKFYFRIADNILKQANIFRAEKNVIDLYVMLLRFSS 60

Query: 61  LVTETIPRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRG 120
           L  ETIP HR++ T+ K  K Y R RLL+ L+ELE+LKP VQ+++DE+  K K + N   
Sbjct: 61  LALETIPSHRDYRTSLKSNKEYLRMRLLDVLTELEKLKPVVQQRIDELYPKLKPRYN--- 120

Query: 121 NQHHNGSRSLEWDSGGKQWLTYNGTAKDGRSAAREIARQGSGPQQFSYSRPIHIPL---- 180
            Q H  + SL W S  K        AK            GS  QQF  + P+        
Sbjct: 121 VQAHPANGSLGWSSAVKPSFNSYDHAKVRNPPGHNSGYMGSRGQQFLNAAPLEERFRKMS 180

Query: 181 ----PKEETLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQQSKETKLTI 240
               P EETLS+HSILGP GL  QWQPP  D  VQYP  +D SP+ IPS QQ  ++K  I
Sbjct: 181 VNFRPNEETLSKHSILGPGGLSAQWQPPKYDTKVQYPSNIDFSPVVIPSFQQLVDSKPMI 240

Query: 241 EKDLGNVEKERSDTDSICTQNEDPQIHNAMERGSLISFEKVEMPAPIEIVRQPSPPPVLA 300
                N E E+   +     NE  Q +   E  S+ISFE+ E      ++RQPSPPPVLA
Sbjct: 241 TNG-SNDEPEKPIVEPSVASNEKIQKNYTEELSSMISFEEPESVNENNLIRQPSPPPVLA 300

Query: 301 EVQDLIPAMSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMRLAKSNTAKNLE 360
           EVQDL+PA+ P+V E EC  + SL D  +  E+ ++LHI+T+MMD+FMRLAKSNT KNLE
Sbjct: 301 EVQDLVPALCPEVREPECMIENSLPDESLRSESPLELHIATSMMDTFMRLAKSNTKKNLE 360

Query: 361 TCGVLAGSLKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLFPLGWIHTHPSQ 420
           TCG+LAGSLKNRKFY+TALI+PKQESTSD+CQ TNEEEIFEVQDK+SLFPLGWIHTHP+Q
Sbjct: 361 TCGILAGSLKNRKFYITALIIPKQESTSDSCQATNEEEIFEVQDKQSLFPLGWIHTHPTQ 420

Query: 421 SCFMSSVDVHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSVIRQCQQRGFHP 480
           SCFMSS+DVHTHYSYQ+MLPEAVAIVMAP+DS+R HGIFRLTTPGGM+VIR C +RGFH 
Sbjct: 421 SCFMSSIDVHTHYSYQIMLPEAVAIVMAPQDSSRNHGIFRLTTPGGMTVIRNCDRRGFHA 480

Query: 481 HGQPPDGGPIYKTCTDVYMDPNLKFDVIDLR 504
           H  P DGGPIY TC +VYM+PNLKFDVIDLR
Sbjct: 481 HSSPEDGGPIYNTCKEVYMNPNLKFDVIDLR 507

[Close]

BLAST of MS021649 vs. TAIR 10
Match: AT4G16144.1 (associated molecule with the SH3 domain of STAM 3 )

HSP 1 Score: 403.7 bits (1036), Expect = 2.2e-112
Identity = 232/522 (44.44%), Postives = 315/522 (60.34%), Query Frame = 0


Query: 7   RINIAASTRRLDVDNRFPLRYYYRVADNILKQADIFRAEKNIIDLYVMLMRFSSLVTETI 66
           +I++    R ++VDNR PLR YYR+ADN+L+QA I+R EKN++DLY+ML+R+SSL++ETI
Sbjct: 2   KIDLNKVAREIEVDNRIPLRNYYRIADNLLRQASIYREEKNVVDLYIMLLRYSSLISETI 61

Query: 67  PRHREHGTTPKYQKNYFRKRLLNALSELEELKPAVQRKVDEINGKQKHQVNGRGNQHHNG 126
           P HR++  +   ++   RKRL   ++ELE LKP   + VD++N     +V     Q  + 
Sbjct: 62  PFHRDYQASLPQERLGSRKRLRAVINELESLKPEFNQLVDKLN-----RVEDESRQDGSD 121

Query: 127 SRSLEWDSGGKQW-----------------------LTYNGTAKDGRSAAREIARQGSGP 186
              + + S   +W                        TYN       +  +         
Sbjct: 122 LPVVSYSSDAVEWPPAHKASYSRPDINKPLPTSQPSWTYNNNLTSSSNRTQI-------D 181

Query: 187 QQFSYSRPIHIPLPKEETLSRHSILGPNGLHGQWQPPIVDKGVQYPRILDLSPIEIPSLQ 246
           QQF       +P P + TLSRHS LGPNGL  Q   P  +  VQYP   D    +   L 
Sbjct: 182 QQFQKLSFDFLP-PNQATLSRHSFLGPNGLKRQMVAPKSEIKVQYPSNTDWGSADNSGLI 241

Query: 247 QSKETKLTIEKDLGNVEKERSDTDSICTQNEDP-QIHNAMERGSLISFEKVEMPAPIEIV 306
           ++  +  +    L    +E S  +S+ + ++   Q H+       IS +  E P     +
Sbjct: 242 EAGPS--SSSASLNGDSQEVSTLNSVLSLDDGRWQRHSEAVNSQFIS-DATEDPFQFVGM 301

Query: 307 RQPSPPPVLAEV-QDLIPAMSPQVPEVECARDISLSDGFVHPEASMQLHISTTMMDSFMR 366
           +QPSPPPVLA+V Q+L      +V +      I   +G     +   LH+   +MD F+R
Sbjct: 302 KQPSPPPVLAQVHQELAQICPSKVADPRPGPAIPSLEGKEGSNSYQHLHVPVRIMDDFLR 361

Query: 367 LAKSNTAKNLETCGVLAGSLKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQDKRSLF 426
           LA+SNT +NLETCGVLAGSLKNR F++T LI+PKQESTSD+CQT NEEEIFEVQD+ SLF
Sbjct: 362 LARSNTERNLETCGVLAGSLKNRVFHITTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLF 421

Query: 427 PLGWIHTHPSQSCFMSSVDVHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTTPGGMSV 486
           PLGWIHTHP+Q+CFMSSVD+HTHYSYQ+MLPEAVAIVMAP D +  HGIF L+ P G+SV
Sbjct: 422 PLGWIHTHPTQTCFMSSVDLHTHYSYQIMLPEAVAIVMAPTDESTPHGIFHLSDPSGVSV 481

Query: 487 IRQCQQRGFHPHGQPPDGGPIYKTCTDVYMDPNLKFDVIDLR 504
           IR CQQRGFHPH +  DG PIY+ C+ V+++  LK++V+DLR
Sbjct: 482 IRNCQQRGFHPHEESEDGNPIYEHCSHVFLNAKLKYEVLDLR 507

[Close]

BLAST of MS021649 vs. TAIR 10
Match: AT1G10600.1 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 247.7 bits (631), Expect = 2.0e-65
Identity = 121/228 (53.07%), Postives = 163/228 (71.49%), Query Frame = 0


Query: 281 IVRQPSPPPVLAEVQDLIPAMSPQVPEVECARDISLSDGFVHPEAS-----MQLHISTTM 340
           +V   SP P L+ V++ +   S  V  V     IS +D   H E+S       +HIS  +
Sbjct: 1   MVTLSSPSPSLSCVEN-VTCKSSHVSRVL----ISGTDNINHGESSEAKILRDVHISERL 60

Query: 341 MDSFMRLAKSNTAKNLETCGVLAGSLKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQ 400
           ++ F  LA+ NT K+LETCG LA  L+   FYVT LI+PKQESTS++CQ  NE E+F +Q
Sbjct: 61  LEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQESTSNSCQAMNEVEVFSIQ 120

Query: 401 DKRSLFPLGWIHTHPSQSCFMSSVDVHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTT 460
           ++R L+P+GWIHTHPSQ CFMSSVD+HTHYSYQVM+PEA AIV+AP DS++++GIF+LT 
Sbjct: 121 NERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPTDSSKSYGIFKLTD 180

Query: 461 PGGMSVIRQCQQRGFHPHGQPPDGGPIYKTCTDVYMDPNLKFDVIDLR 504
           PGGM V+R C + GFHPH +P DG P+Y+ C++VY + NL+F++ DLR
Sbjct: 181 PGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDLR 223

[Close]

BLAST of MS021649 vs. TAIR 10
Match: AT1G10600.3 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 241.9 bits (616), Expect = 1.1e-63
Identity = 121/228 (53.07%), Postives = 161/228 (70.61%), Query Frame = 0


Query: 281 IVRQPSPPPVLAEVQDLIPAMSPQVPEVECARDISLSDGFVHPEAS-----MQLHISTTM 340
           +V   SP P L+ V++ +   S  V  V     IS +D   H E+S       +HIS  +
Sbjct: 1   MVTLSSPSPSLSCVEN-VTCKSSHVSRVL----ISGTDNINHGESSEAKILRDVHISERL 60

Query: 341 MDSFMRLAKSNTAKNLETCGVLAGSLKNRKFYVTALIVPKQESTSDTCQTTNEEEIFEVQ 400
           ++ F  LA+ NT K+LETCG LA  L+   FYVT LI+PKQESTS++CQ  NE E+F +Q
Sbjct: 61  LEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQESTSNSCQAMNEVEVFSIQ 120

Query: 401 DKRSLFPLGWIHTHPSQSCFMSSVDVHTHYSYQVMLPEAVAIVMAPKDSARTHGIFRLTT 460
           ++R L+P+GWIHTHPSQ CFMSSVD+HTHYSYQVM+PEA AIV+AP DS+  +GIF+LT 
Sbjct: 121 NERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPTDSS-NYGIFKLTD 180

Query: 461 PGGMSVIRQCQQRGFHPHGQPPDGGPIYKTCTDVYMDPNLKFDVIDLR 504
           PGGM V+R C + GFHPH +P DG P+Y+ C++VY + NL+F++ DLR
Sbjct: 181 PGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDLR 222

[Close]

BLAST of MS021649 vs. TAIR 10
Match: AT1G10600.2 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 187.6 bits (475), Expect = 2.5e-47
Identity = 76/121 (62.81%), Postives = 101/121 (83.47%), Query Frame = 0


Query: 383 CQTTNEEEIFEVQDKRSLFPLGWIHTHPSQSCFMSSVDVHTHYSYQVMLPEAVAIVMAPK 442
           CQ  NE E+F +Q++R L+P+GWIHTHPSQ CFMSSVD+HTHYSYQVM+PEA AIV+AP 
Sbjct: 46  CQAMNEVEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPT 105

Query: 443 DSARTHGIFRLTTPGGMSVIRQCQQRGFHPHGQPPDGGPIYKTCTDVYMDPNLKFDVIDL 502
           DS++++GIF+LT PGGM V+R C + GFHPH +P DG P+Y+ C++VY + NL+F++ DL
Sbjct: 106 DSSKSYGIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDL 165

Query: 503 R 504
           R
Sbjct: 166 R 166

The following BLAST results are available for this feature:

BLAST of MS021649 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137393.1	8.8e-289	99.40	AMSH-like ubiquitin thioesterase 1 [Momordica charantia]	[more]
XP_022994179.1	1.4e-257	90.06	AMSH-like ubiquitin thioesterase 1 isoform X2 [Cucurbita maxima]	[more]
XP_022924125.1	6.8e-257	89.46	AMSH-like ubiquitin thioesterase 1 [Cucurbita moschata]	[more]
XP_022955249.1	8.9e-257	89.66	AMSH-like ubiquitin thioesterase 1 isoform X1 [Cucurbita moschata]	[more]
KAG6584355.1	1.2e-256	89.07	AMSH-like ubiquitin thioesterase 1, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of MS021649 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYB5	4.9e-165	60.08	AMSH-like ubiquitin thioesterase 1 OS=Arabidopsis thaliana OX=3702 GN=AMSH1 PE=2...	[more]
Q5PNU3	3.1e-111	44.44	AMSH-like ubiquitin thioesterase 3 OS=Arabidopsis thaliana OX=3702 GN=AMSH3 PE=1...	[more]
Q6NKP9	2.9e-64	53.07	AMSH-like ubiquitin thioesterase 2 OS=Arabidopsis thaliana OX=3702 GN=AMSH2 PE=2...	[more]
Q9P371	1.5e-44	51.12	AMSH-like protease sst2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O...	[more]
Q54Q40	2.8e-43	50.29	Probable ubiquitin thioesterase DG1039 OS=Dictyostelium discoideum OX=44689 GN=D...	[more]

BLAST of MS021649 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CA83	4.3e-289	99.40	AMSH-like ubiquitin thioesterase 1 OS=Momordica charantia OX=3673 GN=LOC11100885...	[more]
A0A6J1K266	6.7e-258	90.06	AMSH-like ubiquitin thioesterase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1EDX3	3.3e-257	89.46	AMSH-like ubiquitin thioesterase 1 OS=Cucurbita moschata OX=3662 GN=LOC111431654...	[more]
A0A6J1GVL2	4.3e-257	89.66	AMSH-like ubiquitin thioesterase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1KK10	1.6e-256	89.26	AMSH-like ubiquitin thioesterase 1 OS=Cucurbita maxima OX=3661 GN=LOC111495308 P...	[more]

BLAST of MS021649 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48790.1	3.5e-166	60.08	associated molecule with the SH3 domain of STAM 1	[more]
AT4G16144.1	2.2e-112	44.44	associated molecule with the SH3 domain of STAM 3	[more]
AT1G10600.1	2.0e-65	53.07	associated molecule with the SH3 domain of STAM 2	[more]
AT1G10600.3	1.1e-63	53.07	associated molecule with the SH3 domain of STAM 2	[more]
AT1G10600.2	2.5e-47	62.81	associated molecule with the SH3 domain of STAM 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 84..111
None	No IPR available	COILS	Coil	Coil	coord: 222..242
None	No IPR available	GENE3D	1.20.58.80		coord: 2..129 e-value: 1.2E-29 score: 104.7
None	No IPR available	GENE3D	3.40.140.10	Cytidine Deaminase, domain 2	coord: 319..503 e-value: 2.0E-70 score: 238.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 114..131
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 109..131
None	No IPR available	PANTHER	PTHR12947	AMSH-LIKE PROTEASE	coord: 1..503
None	No IPR available	PANTHER	PTHR12947:SF13	AMSH-LIKE UBIQUITIN THIOESTERASE 1	coord: 1..503
None	No IPR available	SUPERFAMILY	102712	JAB1/MPN domain	coord: 327..459
IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	SMART	SM00232	pad1_6	coord: 328..456 e-value: 4.4E-18 score: 76.1
IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	PFAM	PF01398	JAB	coord: 327..432 e-value: 1.1E-17 score: 64.1
IPR015063	USP8 dimerisation domain	PFAM	PF08969	USP8_dimer	coord: 10..100 e-value: 2.7E-11 score: 43.6
IPR037518	MPN domain	PROSITE	PS50249	MPN	coord: 329..459 score: 32.439381
IPR044098	STAMBP/STALP-like, MPN domain	CDD	cd08066	MPN_AMSH_like	coord: 328..503 e-value: 1.51624E-109 score: 320.696

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021649.1	MS021649.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016579	protein deubiquitination
biological_process	GO:0070536	protein K63-linked deubiquitination
molecular_function	GO:0070122	isopeptidase activity
molecular_function	GO:0061578	Lys63-specific deubiquitinase activity
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0005515	protein binding