MS021648 (gene) Bitter gourd (TR) v1

Overview


Name	MS021648
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	40S ribosomal protein S7
Location	scaffold348: 49696 .. 51378 (+)
RNA-Seq Expression	MS021648
Synteny	MS021648

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATACGGCCCTGAAAAAGATCCACAAGGACAAGGATGTTGAACCTTCTGAATTCGAGGAGTCTGTTGCTCAGGTTGTTTGAGTAAAGATCAGAAATCTTAATGTTTATTCAATTGTCTTTTATTATCTGCATTTCTGATGTTTGCAAAATTTTGCAGGCATTCTTTGATTTGGAAAACACGAATTCGGAGCTGAAAAGTGACCTGAAGGATCTGTACATTAATTCAGCACTGTAAGTGTACTTGGTTGACATTTAAATGACTTGTTTTTATCACAATGATCAGTATGTTATTTCTCAATTCTCATTATGGTAACATTGATGTCTTATGCATTGCAGTCAAGTTGACGTTTCTGGAAACAGGAAGGCTGTCGTTATCTATGTTCCCTTTAGATTGAGGAAGGCTTTCCGCAAGATTCAGTTGAGGCTTGTTCGTGAGCTAGAAAAGAAATTCAGCGGAAAGGTAATGAAAGGTGTATTTTTGCTTTTGTAATCTCTTTTAGTTCTTCAATGGAGCAGCATCAAGTTTCTAGTGTTTATCGTCAGCTAACTTGCTAGATGATATTAGTGACATTTGGATAATTATTTTTAGGTTCAAGAGAGATGTTTTGCAAATTTCCACATTGTCTTTTATGTATGTTATTTCATGCAACTGAATGACCTCTTGCTTTACCCTACGTATTATTCATGACATAGTAAAAATTTATTAATAAGCAGCTTAGTTAGACATCAATTATTAGAGTGATGCTATTTCTCCATGATATTCTCTTGTTTATGGATAGAGATGGACGTGTATACAAGTTGGTCATATTTAAGGTGTATGTTTTCTTGCTTCAATTTCTTATGTGATTGTATGTCAGGATGTTATTCTGATTGCTAACCGGAGAATCTTGAGACCTCCAAAGAAAGGGTCAGCTGTTCAAAGGCCTCGCACTCGTACTCTCACTTCTGTGCATGAAGCAATGCTAGAAGACATTGTTTTGCCCGCCGAGATTGTTGGGAAACGGATCAGATACAGGGTTGATGGATCGAAAATCATGAAGGTAAATGCATTAGAAGATAATTTCCTTATTTCATCCACGTAGCTAGTATGAAAATGAAAAGATGTGTGTTATTTTTTTTTTATAATCACATTTTCTCAGTAGATTTTTAGATTAAATTGTTAAGATATCTATTTTACTTCATCTACATAATTCTATTTATTGGAAGGCTCCATAAATACTATCCTATCATCTGACTTTACTGTTTAGTCTGTGTAAGTAGGTTCACTTGGCTTGTGGCCAAAACTGTTGCCAAACTGAATTAAAAACCAATGCTGAAACCTACTGCTTGAAACTTTGAGCCAGCCAACTAGTTTTTTATTTTTGAATTTTAAACTCCTCAAACCTTCCAATGTATGTTGCTCAATTCAACCAACCCGTTCTTTTCTCCGAGTTTTTGCTTCCATCCCCCTATTACTGTTGTCAGCCACACTTGTGTCTCATGGTGGTGAATTATTATCCCCTACTTGCTTACTGTGTATCTTCTATTACAAGTCTCCTGACCCGTTGGATCTTCTTGGGGCAGGTTTACTTGGACCCTAAGGAGCGGAACAACACCGAGTACAAGTTGGAGAGTTTTGCTGCAGTTTACAGGAAGCTTTCTGGTAAAGATGTTGCCTTTGAGTTCCCTATTACAGAGGCA

mRNA sequence

ATGTATACGGCCCTGAAAAAGATCCACAAGGACAAGGATGTTGAACCTTCTGAATTCGAGGAGTCTGTTGCTCAGGCATTCTTTGATTTGGAAAACACGAATTCGGAGCTGAAAAGTGACCTGAAGGATCTGTACATTAATTCAGCACTTCAAGTTGACGTTTCTGGAAACAGGAAGGCTGTCGTTATCTATGTTCCCTTTAGATTGAGGAAGGCTTTCCGCAAGATTCAGTTGAGGCTTGTTCGTGAGCTAGAAAAGAAATTCAGCGGAAAGGATGTTATTCTGATTGCTAACCGGAGAATCTTGAGACCTCCAAAGAAAGGGTCAGCTGTTCAAAGGCCTCGCACTCGTACTCTCACTTCTGTGCATGAAGCAATGCTAGAAGACATTGTTTTGCCCGCCGAGATTGTTGGGAAACGGATCAGATACAGGGTTGATGGATCGAAAATCATGAAGGTTTACTTGGACCCTAAGGAGCGGAACAACACCGAGTACAAGTTGGAGAGTTTTGCTGCAGTTTACAGGAAGCTTTCTGGTAAAGATGTTGCCTTTGAGTTCCCTATTACAGAGGCA

Coding sequence (CDS)

ATGTATACGGCCCTGAAAAAGATCCACAAGGACAAGGATGTTGAACCTTCTGAATTCGAGGAGTCTGTTGCTCAGGCATTCTTTGATTTGGAAAACACGAATTCGGAGCTGAAAAGTGACCTGAAGGATCTGTACATTAATTCAGCACTTCAAGTTGACGTTTCTGGAAACAGGAAGGCTGTCGTTATCTATGTTCCCTTTAGATTGAGGAAGGCTTTCCGCAAGATTCAGTTGAGGCTTGTTCGTGAGCTAGAAAAGAAATTCAGCGGAAAGGATGTTATTCTGATTGCTAACCGGAGAATCTTGAGACCTCCAAAGAAAGGGTCAGCTGTTCAAAGGCCTCGCACTCGTACTCTCACTTCTGTGCATGAAGCAATGCTAGAAGACATTGTTTTGCCCGCCGAGATTGTTGGGAAACGGATCAGATACAGGGTTGATGGATCGAAAATCATGAAGGTTTACTTGGACCCTAAGGAGCGGAACAACACCGAGTACAAGTTGGAGAGTTTTGCTGCAGTTTACAGGAAGCTTTCTGGTAAAGATGTTGCCTTTGAGTTCCCTATTACAGAGGCA

Protein sequence

MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKAVVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLTSVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGKDVAFEFPITEA

Homology

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BLAST of MS021648 vs. NCBI nr
Match: XP_022137245.1 (40S ribosomal protein S7-like [Momordica charantia])

HSP 1 Score: 366.3 bits (939), Expect = 1.6e-97
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA
Sbjct: 49  MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 108

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT
Sbjct: 109 VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 168

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK
Sbjct: 169 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 228

Query: 181 DVAFEFPITEA 192
           DVAFEFPITEA
Sbjct: 229 DVAFEFPITEA 239

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BLAST of MS021648 vs. NCBI nr
Match: XP_004137478.1 (40S ribosomal protein S7 [Cucumis sativus] >KGN64124.1 hypothetical protein Csa_014367 [Cucumis sativus])

HSP 1 Score: 355.9 bits (912), Expect = 2.2e-94
Identity = 185/191 (96.86%), Postives = 188/191 (98.43%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MYTALKKIHKDKDVE SEFEE+VAQA FDLENTNSELKSDLKDLYINSALQVDVSGNRKA
Sbjct: 1   MYTALKKIHKDKDVEASEFEETVAQALFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVIL+ANRRILRPPKKGSAVQRPRTRTLT
Sbjct: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILVANRRILRPPKKGSAVQRPRTRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLEDIVLPAEIVGKR RYRVDGSKIMKV+LDPKERNNTEYKLESFAAVYRKLSGK
Sbjct: 121 SVHEAMLEDIVLPAEIVGKRTRYRVDGSKIMKVFLDPKERNNTEYKLESFAAVYRKLSGK 180

Query: 181 DVAFEFPITEA 192
           DVAFEFPITEA
Sbjct: 181 DVAFEFPITEA 191

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BLAST of MS021648 vs. NCBI nr
Match: XP_008458552.1 (PREDICTED: 40S ribosomal protein S7 [Cucumis melo] >XP_008458626.1 PREDICTED: 40S ribosomal protein S7 [Cucumis melo] >XP_038893522.1 40S ribosomal protein S7-like [Benincasa hispida] >TYJ98994.1 40S ribosomal protein S7 [Cucumis melo var. makuwa])

HSP 1 Score: 354.4 bits (908), Expect = 6.3e-94
Identity = 184/191 (96.34%), Postives = 187/191 (97.91%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MYTALKKIHKDKD E SEFEE+VAQA FDLENTNSELKSDLKDLYINSALQVDVSGNRKA
Sbjct: 1   MYTALKKIHKDKDAEASEFEETVAQALFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVIL+ANRRILRPPKKGSAVQRPRTRTLT
Sbjct: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILVANRRILRPPKKGSAVQRPRTRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLEDIVLPAEIVGKR RYRVDGSKIMKV+LDPKERNNTEYKLESFAAVYRKLSGK
Sbjct: 121 SVHEAMLEDIVLPAEIVGKRTRYRVDGSKIMKVFLDPKERNNTEYKLESFAAVYRKLSGK 180

Query: 181 DVAFEFPITEA 192
           DVAFEFPITEA
Sbjct: 181 DVAFEFPITEA 191

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BLAST of MS021648 vs. NCBI nr
Match: XP_022955302.1 (40S ribosomal protein S7-like [Cucurbita moschata] >XP_022955303.1 40S ribosomal protein S7-like [Cucurbita moschata] >XP_022994526.1 40S ribosomal protein S7-like [Cucurbita maxima] >XP_022994527.1 40S ribosomal protein S7-like [Cucurbita maxima] >XP_023541066.1 40S ribosomal protein S7-like [Cucurbita pepo subsp. pepo] >XP_023541067.1 40S ribosomal protein S7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 353.2 bits (905), Expect = 1.4e-93
Identity = 183/191 (95.81%), Postives = 187/191 (97.91%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           M+TALKKIHKDKDVEPSEFEE+VAQA FDLENTNSELKSDLKDLYINSALQVDVSGNRKA
Sbjct: 1   MFTALKKIHKDKDVEPSEFEETVAQALFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSA QRPRTRTLT
Sbjct: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAAQRPRTRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLEDIVLPAEIVGKR RYRVDGSKIMKV+LDPKERNNTEYKLESF+AVYRKLSGK
Sbjct: 121 SVHEAMLEDIVLPAEIVGKRTRYRVDGSKIMKVFLDPKERNNTEYKLESFSAVYRKLSGK 180

Query: 181 DVAFEFPITEA 192
           DVAFEFPI EA
Sbjct: 181 DVAFEFPIVEA 191

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BLAST of MS021648 vs. NCBI nr
Match: KAG6572941.1 (40S ribosomal protein S7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 353.2 bits (905), Expect = 1.4e-93
Identity = 183/191 (95.81%), Postives = 187/191 (97.91%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           M+TALKKIHKDKDVEPSEFEE+VAQA FDLENTNSELKSDLKDLYINSALQVDVSGNRKA
Sbjct: 40  MFTALKKIHKDKDVEPSEFEETVAQALFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 99

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSA QRPRTRTLT
Sbjct: 100 VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAAQRPRTRTLT 159

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLEDIVLPAEIVGKR RYRVDGSKIMKV+LDPKERNNTEYKLESF+AVYRKLSGK
Sbjct: 160 SVHEAMLEDIVLPAEIVGKRTRYRVDGSKIMKVFLDPKERNNTEYKLESFSAVYRKLSGK 219

Query: 181 DVAFEFPITEA 192
           DVAFEFPI EA
Sbjct: 220 DVAFEFPIVEA 230

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BLAST of MS021648 vs. ExPASy Swiss-Prot
Match: Q9ZNS1 (40S ribosomal protein S7 OS=Avicennia marina OX=82927 GN=RPS7 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 1.6e-84
Identity = 156/190 (82.11%), Postives = 177/190 (93.16%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MYTAL+KIHKDKD EP+EFEE+VAQA FD ENTN E+KSDLKDLYINSALQ+DVSG +KA
Sbjct: 1   MYTALQKIHKDKDAEPTEFEENVAQALFDFENTNQEIKSDLKDLYINSALQIDVSGGKKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVI+VP+RLRK+FRKI  RLVRELEKKFSGK+V+LIA RRILRPPKKGSAVQRPR+RTLT
Sbjct: 61  VVIHVPYRLRKSFRKIHPRLVRELEKKFSGKEVVLIATRRILRPPKKGSAVQRPRSRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           +VH+AMLEDIV PAEIVGKR+RYR+DGSKIMKV+LDPK +N+TE KLE+FA VYRKLSGK
Sbjct: 121 AVHDAMLEDIVYPAEIVGKRVRYRLDGSKIMKVFLDPKAKNDTENKLETFAGVYRKLSGK 180

Query: 181 DVAFEFPITE 191
           DV FEFP+TE
Sbjct: 181 DVVFEFPLTE 190

[Close]

BLAST of MS021648 vs. ExPASy Swiss-Prot
Match: Q9XH45 (40S ribosomal protein S7 OS=Brassica oleracea OX=3712 GN=RPS7 PE=2 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 5.7e-82
Identity = 151/190 (79.47%), Postives = 168/190 (88.42%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           M++A  KIHKDK V P+EFEE VAQAFFDLENTN ELKSDLKDLYIN A+ +D++GNRKA
Sbjct: 1   MFSAQNKIHKDKGVAPTEFEERVAQAFFDLENTNQELKSDLKDLYINQAVSMDIAGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKI  RLVRELEKKFSG DVI +A RRI+RPPKKGSAVQRPR RTLT
Sbjct: 61  VVIYVPFRLRKAFRKIHSRLVRELEKKFSGNDVIFVATRRIMRPPKKGSAVQRPRNRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLED+  PAEIVGKR RYRVDG+KIMKVYL+PKERNNTEYKLE+   VYRKL+GK
Sbjct: 121 SVHEAMLEDVAYPAEIVGKRTRYRVDGTKIMKVYLEPKERNNTEYKLETMVGVYRKLTGK 180

Query: 181 DVAFEFPITE 191
           DV FE+P+ +
Sbjct: 181 DVVFEYPVAD 190

[Close]

BLAST of MS021648 vs. ExPASy Swiss-Prot
Match: Q9C514 (40S ribosomal protein S7-1 OS=Arabidopsis thaliana OX=3702 GN=RPS7A PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 1.3e-81
Identity = 150/191 (78.53%), Postives = 176/191 (92.15%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           M++A  KIHK+K VE SE +E VAQAFFDLENTN ELKS+LKDLY+NSA+QVD+SG RKA
Sbjct: 1   MFSAQHKIHKEKGVELSELDEQVAQAFFDLENTNQELKSELKDLYVNSAVQVDISGGRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           +V+ VP+RLRKA+RKI +RLVRELEKKFSGKDVILIA RRI+RPPKKGSA +RPR RTLT
Sbjct: 61  IVVNVPYRLRKAYRKIHVRLVRELEKKFSGKDVILIATRRIVRPPKKGSAAKRPRNRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEA+L+D+VLPAEIVGKR RYR+DG+KIMKV+LDPKERNNTEYK+E+F+AVY+KL+GK
Sbjct: 121 SVHEAILDDVVLPAEIVGKRTRYRLDGTKIMKVFLDPKERNNTEYKVEAFSAVYKKLTGK 180

Query: 181 DVAFEFPITEA 192
           DV FEFPITEA
Sbjct: 181 DVVFEFPITEA 191

[Close]

BLAST of MS021648 vs. ExPASy Swiss-Prot
Match: Q9M885 (40S ribosomal protein S7-2 OS=Arabidopsis thaliana OX=3702 GN=RPS7B PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 3.1e-80
Identity = 149/191 (78.01%), Postives = 167/191 (87.43%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MY+   KIHKDK V P+EFEE V QA FDLENTN ELKS+LKDLYIN A+Q+D+SGNRKA
Sbjct: 1   MYSGQNKIHKDKGVAPTEFEEQVTQALFDLENTNQELKSELKDLYINQAVQMDISGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKI LRLVRELEKKFSGKDVI +A RRI+RPPKKGSAVQRPR RTLT
Sbjct: 61  VVIYVPFRLRKAFRKIHLRLVRELEKKFSGKDVIFVATRRIMRPPKKGSAVQRPRNRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLED+  PAEIVGKR RYR+DG+KIMKV+LD K +N+TEYKLE+   VYRKL+GK
Sbjct: 121 SVHEAMLEDVAYPAEIVGKRTRYRLDGTKIMKVFLDSKLKNDTEYKLETMVGVYRKLTGK 180

Query: 181 DVAFEFPITEA 192
           DV FE+P+ EA
Sbjct: 181 DVVFEYPVIEA 191

[Close]

BLAST of MS021648 vs. ExPASy Swiss-Prot
Match: Q9XET4 (40S ribosomal protein S7 OS=Secale cereale OX=4550 GN=RPS7 PE=2 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 4.1e-80
Identity = 144/190 (75.79%), Postives = 173/190 (91.05%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MYTA KKI KDK +EPSEFE++VAQAFFDLEN N ELKSD+KDLYIN+A Q+DV+GNRKA
Sbjct: 1   MYTARKKIQKDKGLEPSEFEDTVAQAFFDLENGNQELKSDVKDLYINAAFQMDVAGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVI+VP+RLRK FRKI +RLVRELEKKFSGKDV+++A RRI+RPPKKGSAV RPRTRTLT
Sbjct: 61  VVIHVPYRLRKNFRKIHVRLVRELEKKFSGKDVVIVATRRIVRPPKKGSAVVRPRTRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           +VH+ +LED+V PAEIVGKR+RYR+DGSKI+K++LDP ERNNTEYKL++F AVYR+L GK
Sbjct: 121 AVHDGLLEDVVYPAEIVGKRVRYRLDGSKIIKIFLDPMERNNTEYKLDTFTAVYRRLCGK 180

Query: 181 DVAFEFPITE 191
           DV +E+P+ E
Sbjct: 181 DVVYEYPVAE 190

[Close]

BLAST of MS021648 vs. ExPASy TrEMBL
Match: A0A6J1C647 (40S ribosomal protein S7 OS=Momordica charantia OX=3673 GN=LOC111008755 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 7.7e-98
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA
Sbjct: 49  MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 108

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT
Sbjct: 109 VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 168

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK
Sbjct: 169 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 228

Query: 181 DVAFEFPITEA 192
           DVAFEFPITEA
Sbjct: 229 DVAFEFPITEA 239

[Close]

BLAST of MS021648 vs. ExPASy TrEMBL
Match: A0A0A0LSZ7 (40S ribosomal protein S7 OS=Cucumis sativus OX=3659 GN=Csa_1G042360 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.0e-94
Identity = 185/191 (96.86%), Postives = 188/191 (98.43%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MYTALKKIHKDKDVE SEFEE+VAQA FDLENTNSELKSDLKDLYINSALQVDVSGNRKA
Sbjct: 1   MYTALKKIHKDKDVEASEFEETVAQALFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVIL+ANRRILRPPKKGSAVQRPRTRTLT
Sbjct: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILVANRRILRPPKKGSAVQRPRTRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLEDIVLPAEIVGKR RYRVDGSKIMKV+LDPKERNNTEYKLESFAAVYRKLSGK
Sbjct: 121 SVHEAMLEDIVLPAEIVGKRTRYRVDGSKIMKVFLDPKERNNTEYKLESFAAVYRKLSGK 180

Query: 181 DVAFEFPITEA 192
           DVAFEFPITEA
Sbjct: 181 DVAFEFPITEA 191

[Close]

BLAST of MS021648 vs. ExPASy TrEMBL
Match: A0A1S3C8E2 (40S ribosomal protein S7 OS=Cucumis melo OX=3656 GN=LOC103497898 PE=3 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.0e-94
Identity = 184/191 (96.34%), Postives = 187/191 (97.91%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MYTALKKIHKDKD E SEFEE+VAQA FDLENTNSELKSDLKDLYINSALQVDVSGNRKA
Sbjct: 1   MYTALKKIHKDKDAEASEFEETVAQALFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVIL+ANRRILRPPKKGSAVQRPRTRTLT
Sbjct: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILVANRRILRPPKKGSAVQRPRTRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLEDIVLPAEIVGKR RYRVDGSKIMKV+LDPKERNNTEYKLESFAAVYRKLSGK
Sbjct: 121 SVHEAMLEDIVLPAEIVGKRTRYRVDGSKIMKVFLDPKERNNTEYKLESFAAVYRKLSGK 180

Query: 181 DVAFEFPITEA 192
           DVAFEFPITEA
Sbjct: 181 DVAFEFPITEA 191

[Close]

BLAST of MS021648 vs. ExPASy TrEMBL
Match: A0A5D3BIV5 (40S ribosomal protein S7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001980 PE=3 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.0e-94
Identity = 184/191 (96.34%), Postives = 187/191 (97.91%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MYTALKKIHKDKD E SEFEE+VAQA FDLENTNSELKSDLKDLYINSALQVDVSGNRKA
Sbjct: 1   MYTALKKIHKDKDAEASEFEETVAQALFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVIL+ANRRILRPPKKGSAVQRPRTRTLT
Sbjct: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILVANRRILRPPKKGSAVQRPRTRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLEDIVLPAEIVGKR RYRVDGSKIMKV+LDPKERNNTEYKLESFAAVYRKLSGK
Sbjct: 121 SVHEAMLEDIVLPAEIVGKRTRYRVDGSKIMKVFLDPKERNNTEYKLESFAAVYRKLSGK 180

Query: 181 DVAFEFPITEA 192
           DVAFEFPITEA
Sbjct: 181 DVAFEFPITEA 191

[Close]

BLAST of MS021648 vs. ExPASy TrEMBL
Match: A0A6J1JZE1 (40S ribosomal protein S7 OS=Cucurbita maxima OX=3661 GN=LOC111490221 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.8e-94
Identity = 183/191 (95.81%), Postives = 187/191 (97.91%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           M+TALKKIHKDKDVEPSEFEE+VAQA FDLENTNSELKSDLKDLYINSALQVDVSGNRKA
Sbjct: 1   MFTALKKIHKDKDVEPSEFEETVAQALFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSA QRPRTRTLT
Sbjct: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAAQRPRTRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLEDIVLPAEIVGKR RYRVDGSKIMKV+LDPKERNNTEYKLESF+AVYRKLSGK
Sbjct: 121 SVHEAMLEDIVLPAEIVGKRTRYRVDGSKIMKVFLDPKERNNTEYKLESFSAVYRKLSGK 180

Query: 181 DVAFEFPITEA 192
           DVAFEFPI EA
Sbjct: 181 DVAFEFPIVEA 191

[Close]

BLAST of MS021648 vs. TAIR 10
Match: AT1G48830.1 (Ribosomal protein S7e family protein )

HSP 1 Score: 303.9 bits (777), Expect = 9.0e-83
Identity = 150/191 (78.53%), Postives = 176/191 (92.15%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           M++A  KIHK+K VE SE +E VAQAFFDLENTN ELKS+LKDLY+NSA+QVD+SG RKA
Sbjct: 1   MFSAQHKIHKEKGVELSELDEQVAQAFFDLENTNQELKSELKDLYVNSAVQVDISGGRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           +V+ VP+RLRKA+RKI +RLVRELEKKFSGKDVILIA RRI+RPPKKGSA +RPR RTLT
Sbjct: 61  IVVNVPYRLRKAYRKIHVRLVRELEKKFSGKDVILIATRRIVRPPKKGSAAKRPRNRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEA+L+D+VLPAEIVGKR RYR+DG+KIMKV+LDPKERNNTEYK+E+F+AVY+KL+GK
Sbjct: 121 SVHEAILDDVVLPAEIVGKRTRYRLDGTKIMKVFLDPKERNNTEYKVEAFSAVYKKLTGK 180

Query: 181 DVAFEFPITEA 192
           DV FEFPITEA
Sbjct: 181 DVVFEFPITEA 191

[Close]

BLAST of MS021648 vs. TAIR 10
Match: AT1G48830.2 (Ribosomal protein S7e family protein )

HSP 1 Score: 303.9 bits (777), Expect = 9.0e-83
Identity = 150/191 (78.53%), Postives = 176/191 (92.15%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           M++A  KIHK+K VE SE +E VAQAFFDLENTN ELKS+LKDLY+NSA+QVD+SG RKA
Sbjct: 1   MFSAQHKIHKEKGVELSELDEQVAQAFFDLENTNQELKSELKDLYVNSAVQVDISGGRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           +V+ VP+RLRKA+RKI +RLVRELEKKFSGKDVILIA RRI+RPPKKGSA +RPR RTLT
Sbjct: 61  IVVNVPYRLRKAYRKIHVRLVRELEKKFSGKDVILIATRRIVRPPKKGSAAKRPRNRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEA+L+D+VLPAEIVGKR RYR+DG+KIMKV+LDPKERNNTEYK+E+F+AVY+KL+GK
Sbjct: 121 SVHEAILDDVVLPAEIVGKRTRYRLDGTKIMKVFLDPKERNNTEYKVEAFSAVYKKLTGK 180

Query: 181 DVAFEFPITEA 192
           DV FEFPITEA
Sbjct: 181 DVVFEFPITEA 191

[Close]

BLAST of MS021648 vs. TAIR 10
Match: AT3G02560.1 (Ribosomal protein S7e family protein )

HSP 1 Score: 299.3 bits (765), Expect = 2.2e-81
Identity = 149/191 (78.01%), Postives = 167/191 (87.43%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MY+   KIHKDK V P+EFEE V QA FDLENTN ELKS+LKDLYIN A+Q+D+SGNRKA
Sbjct: 1   MYSGQNKIHKDKGVAPTEFEEQVTQALFDLENTNQELKSELKDLYINQAVQMDISGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKI LRLVRELEKKFSGKDVI +A RRI+RPPKKGSAVQRPR RTLT
Sbjct: 61  VVIYVPFRLRKAFRKIHLRLVRELEKKFSGKDVIFVATRRIMRPPKKGSAVQRPRNRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLED+  PAEIVGKR RYR+DG+KIMKV+LD K +N+TEYKLE+   VYRKL+GK
Sbjct: 121 SVHEAMLEDVAYPAEIVGKRTRYRLDGTKIMKVFLDSKLKNDTEYKLETMVGVYRKLTGK 180

Query: 181 DVAFEFPITEA 192
           DV FE+P+ EA
Sbjct: 181 DVVFEYPVIEA 191

[Close]

BLAST of MS021648 vs. TAIR 10
Match: AT3G02560.2 (Ribosomal protein S7e family protein )

HSP 1 Score: 299.3 bits (765), Expect = 2.2e-81
Identity = 149/191 (78.01%), Postives = 167/191 (87.43%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           MY+   KIHKDK V P+EFEE V QA FDLENTN ELKS+LKDLYIN A+Q+D+SGNRKA
Sbjct: 1   MYSGQNKIHKDKGVAPTEFEEQVTQALFDLENTNQELKSELKDLYINQAVQMDISGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKI LRLVRELEKKFSGKDVI +A RRI+RPPKKGSAVQRPR RTLT
Sbjct: 61  VVIYVPFRLRKAFRKIHLRLVRELEKKFSGKDVIFVATRRIMRPPKKGSAVQRPRNRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLED+  PAEIVGKR RYR+DG+KIMKV+LD K +N+TEYKLE+   VYRKL+GK
Sbjct: 121 SVHEAMLEDVAYPAEIVGKRTRYRLDGTKIMKVFLDSKLKNDTEYKLETMVGVYRKLTGK 180

Query: 181 DVAFEFPITEA 192
           DV FE+P+ EA
Sbjct: 181 DVVFEYPVIEA 191

[Close]

BLAST of MS021648 vs. TAIR 10
Match: AT5G16130.1 (Ribosomal protein S7e family protein )

HSP 1 Score: 292.0 bits (746), Expect = 3.6e-79
Identity = 145/188 (77.13%), Postives = 164/188 (87.23%), Query Frame = 0


Query: 1   MYTALKKIHKDKDVEPSEFEESVAQAFFDLENTNSELKSDLKDLYINSALQVDVSGNRKA 60
           M++A  KI KDK+ EP+E EE VAQA FDLENTN ELKS+LKDLYIN A+ +D+SGNRKA
Sbjct: 1   MFSAQNKIKKDKNAEPTECEEQVAQALFDLENTNQELKSELKDLYINQAVHMDISGNRKA 60

Query: 61  VVIYVPFRLRKAFRKIQLRLVRELEKKFSGKDVILIANRRILRPPKKGSAVQRPRTRTLT 120
           VVIYVPFRLRKAFRKI  RLVRELEKKFSGKDVI +  RRI+RPPKKG+AVQRPR RTLT
Sbjct: 61  VVIYVPFRLRKAFRKIHPRLVRELEKKFSGKDVIFVTTRRIMRPPKKGAAVQRPRNRTLT 120

Query: 121 SVHEAMLEDIVLPAEIVGKRIRYRVDGSKIMKVYLDPKERNNTEYKLESFAAVYRKLSGK 180
           SVHEAMLED+  PAEIVGKR RYR+DGSKIMKV+LD KE+NNTEYKLE+   VYRKL+GK
Sbjct: 121 SVHEAMLEDVAFPAEIVGKRTRYRLDGSKIMKVFLDAKEKNNTEYKLETMVGVYRKLTGK 180

Query: 181 DVAFEFPI 189
           DV FE+P+
Sbjct: 181 DVVFEYPV 188

The following BLAST results are available for this feature:

BLAST of MS021648 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137245.1	1.6e-97	100.00	40S ribosomal protein S7-like [Momordica charantia]	[more]
XP_004137478.1	2.2e-94	96.86	40S ribosomal protein S7 [Cucumis sativus] >KGN64124.1 hypothetical protein Csa_...	[more]
XP_008458552.1	6.3e-94	96.34	PREDICTED: 40S ribosomal protein S7 [Cucumis melo] >XP_008458626.1 PREDICTED: 40...	[more]
XP_022955302.1	1.4e-93	95.81	40S ribosomal protein S7-like [Cucurbita moschata] >XP_022955303.1 40S ribosomal...	[more]
KAG6572941.1	1.4e-93	95.81	40S ribosomal protein S7, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MS021648 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNS1	1.6e-84	82.11	40S ribosomal protein S7 OS=Avicennia marina OX=82927 GN=RPS7 PE=2 SV=1	[more]
Q9XH45	5.7e-82	79.47	40S ribosomal protein S7 OS=Brassica oleracea OX=3712 GN=RPS7 PE=2 SV=1	[more]
Q9C514	1.3e-81	78.53	40S ribosomal protein S7-1 OS=Arabidopsis thaliana OX=3702 GN=RPS7A PE=2 SV=1	[more]
Q9M885	3.1e-80	78.01	40S ribosomal protein S7-2 OS=Arabidopsis thaliana OX=3702 GN=RPS7B PE=2 SV=1	[more]
Q9XET4	4.1e-80	75.79	40S ribosomal protein S7 OS=Secale cereale OX=4550 GN=RPS7 PE=2 SV=1	[more]

BLAST of MS021648 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C647	7.7e-98	100.00	40S ribosomal protein S7 OS=Momordica charantia OX=3673 GN=LOC111008755 PE=3 SV=...	[more]
A0A0A0LSZ7	1.0e-94	96.86	40S ribosomal protein S7 OS=Cucumis sativus OX=3659 GN=Csa_1G042360 PE=3 SV=1	[more]
A0A1S3C8E2	3.0e-94	96.34	40S ribosomal protein S7 OS=Cucumis melo OX=3656 GN=LOC103497898 PE=3 SV=1	[more]
A0A5D3BIV5	3.0e-94	96.34	40S ribosomal protein S7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A6J1JZE1	6.8e-94	95.81	40S ribosomal protein S7 OS=Cucurbita maxima OX=3661 GN=LOC111490221 PE=3 SV=1	[more]

BLAST of MS021648 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48830.1	9.0e-83	78.53	Ribosomal protein S7e family protein	[more]
AT1G48830.2	9.0e-83	78.53	Ribosomal protein S7e family protein	[more]
AT3G02560.1	2.2e-81	78.01	Ribosomal protein S7e family protein	[more]
AT3G02560.2	2.2e-81	78.01	Ribosomal protein S7e family protein	[more]
AT5G16130.1	3.6e-79	77.13	Ribosomal protein S7e family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000554	Ribosomal protein S7e	PFAM	PF01251	Ribosomal_S7e	coord: 7..187 e-value: 1.0E-80 score: 269.9
IPR000554	Ribosomal protein S7e	PANTHER	PTHR11278	40S RIBOSOMAL PROTEIN S7	coord: 2..191
IPR000554	Ribosomal protein S7e	PROSITE	PS00948	RIBOSOMAL_S7E	coord: 79..92
None	No IPR available	PANTHER	PTHR11278:SF19	40S RIBOSOMAL PROTEIN S7	coord: 2..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021648.1	MS021648.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005840	ribosome
molecular_function	GO:0003735	structural constituent of ribosome