MS021375 (gene) Bitter gourd (TR) v1

Overview


Name	MS021375
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	photosynthetic NDH subunit of lumenal location 3, chloroplastic
Location	scaffold358: 1518205 .. 1519062 (-)
RNA-Seq Expression	MS021375
Synteny	MS021375

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCGTCTCGCAAATTTAAACGGCGTCTCAGACAAATTCTCCGCCATCCCCAAGCTTCCAAAGCTTCAAAACCCTCGGAAGAGACTCAAAATAATTGGGTTTCTCGGGAAACAAACAGAGGTTTCTTCTCATCACAGCTCATTCACAAGAAGATTGGCTTTAACCAGCATCGTCTCTGTTGCTCTGTTTGGCAATGCATCCGTCGAGATCGCTTCCGCTAACGAGTACTGGCTCGATGGCCCTTTGCCTGTGCCGTCTGTTTACAACAGTAAGCTAGCTTTCTGATTGAAGAGTTTTGAAGCTTTCTGTTTCTGAGCTTGTTTCTTTATCCGGGTGCAACTCGAAATCGAAAAAAATCGAGGACTGACTCGAATGAATTTCTTGTTACAGACATTGCGAATGAGAAGACGGGGACCCGATCGTTTCTGAAGACGGGGATTTACATAGCCAACATAGGGACAGAAGGAAGAAAATATAGACTAAAGAGATATGCCTTTGATCTTCTGGCTATGGCGGATTTGATCGGGAAAGATACTTTGAACTATGTAAGGAAGTATCTTCGACTGAAATCGACTTTCATGTACTACGATTTCGACAATGTCATCTCGGCAGCTGCAGATGGTGAGAAGGAGCCTCTAACCGAGCTTGCGAACAGATTGTTCGACAACTTCGAAAAGGTAAAAAGACGAGAAAGTTTACAAATTTCTGCAAGTAGTCTGCAGATTTTCAAGCTGAAGTAGCTTGTTTTGTGTTTTGCAGCTTGAAGATGCAGCAAAACAGAAGAATCTGAGTGAAACTGAATCCTATTATCAGCAGACAACTCCTATACTTCAAGAAGTCATGGATAGAATGGCT

mRNA sequence

ATGGCTCGTCTCGCAAATTTAAACGGCGTCTCAGACAAATTCTCCGCCATCCCCAAGCTTCCAAAGCTTCAAAACCCTCGGAAGAGACTCAAAATAATTGGGTTTCTCGGGAAACAAACAGAGGTTTCTTCTCATCACAGCTCATTCACAAGAAGATTGGCTTTAACCAGCATCGTCTCTGTTGCTCTGTTTGGCAATGCATCCGTCGAGATCGCTTCCGCTAACGAGTACTGGCTCGATGGCCCTTTGCCTGTGCCGTCTGTTTACAACAACATTGCGAATGAGAAGACGGGGACCCGATCGTTTCTGAAGACGGGGATTTACATAGCCAACATAGGGACAGAAGGAAGAAAATATAGACTAAAGAGATATGCCTTTGATCTTCTGGCTATGGCGGATTTGATCGGGAAAGATACTTTGAACTATGTAAGGAAGTATCTTCGACTGAAATCGACTTTCATGTACTACGATTTCGACAATGTCATCTCGGCAGCTGCAGATGGTGAGAAGGAGCCTCTAACCGAGCTTGCGAACAGATTGTTCGACAACTTCGAAAAGCTTGAAGATGCAGCAAAACAGAAGAATCTGAGTGAAACTGAATCCTATTATCAGCAGACAACTCCTATACTTCAAGAAGTCATGGATAGAATGGCT

Coding sequence (CDS)

ATGGCTCGTCTCGCAAATTTAAACGGCGTCTCAGACAAATTCTCCGCCATCCCCAAGCTTCCAAAGCTTCAAAACCCTCGGAAGAGACTCAAAATAATTGGGTTTCTCGGGAAACAAACAGAGGTTTCTTCTCATCACAGCTCATTCACAAGAAGATTGGCTTTAACCAGCATCGTCTCTGTTGCTCTGTTTGGCAATGCATCCGTCGAGATCGCTTCCGCTAACGAGTACTGGCTCGATGGCCCTTTGCCTGTGCCGTCTGTTTACAACAACATTGCGAATGAGAAGACGGGGACCCGATCGTTTCTGAAGACGGGGATTTACATAGCCAACATAGGGACAGAAGGAAGAAAATATAGACTAAAGAGATATGCCTTTGATCTTCTGGCTATGGCGGATTTGATCGGGAAAGATACTTTGAACTATGTAAGGAAGTATCTTCGACTGAAATCGACTTTCATGTACTACGATTTCGACAATGTCATCTCGGCAGCTGCAGATGGTGAGAAGGAGCCTCTAACCGAGCTTGCGAACAGATTGTTCGACAACTTCGAAAAGCTTGAAGATGCAGCAAAACAGAAGAATCTGAGTGAAACTGAATCCTATTATCAGCAGACAACTCCTATACTTCAAGAAGTCATGGATAGAATGGCT

Protein sequence

MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVSVALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA

Homology

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BLAST of MS021375 vs. NCBI nr
Match: XP_022139520.1 (photosynthetic NDH subunit of lumenal location 3, chloroplastic [Momordica charantia])

HSP 1 Score: 427.9 bits (1099), Expect = 5.1e-116
Identity = 217/218 (99.54%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60
           MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS
Sbjct: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60

Query: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120
           VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR
Sbjct: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120

Query: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRL 180
           LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEK+PLTELANRL
Sbjct: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKQPLTELANRL 180

Query: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA
Sbjct: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 218

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BLAST of MS021375 vs. NCBI nr
Match: XP_022940191.1 (photosynthetic NDH subunit of lumenal location 3, chloroplastic [Cucurbita moschata])

HSP 1 Score: 392.5 bits (1007), Expect = 2.4e-105
Identity = 199/218 (91.28%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60
           MARLANLNGVS+KFSAIPKL KLQ  RKR KI+G L KQ EVSSH  S TRRLALTS+ S
Sbjct: 1   MARLANLNGVSEKFSAIPKLSKLQKTRKRSKIMGLLEKQAEVSSHEVSLTRRLALTSLTS 60

Query: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120
           VALFGNASVEIASANEYWLDGPLP+PSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR
Sbjct: 61  VALFGNASVEIASANEYWLDGPLPIPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120

Query: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRL 180
           LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAA DGEK+PLT+LANRL
Sbjct: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAEDGEKQPLTDLANRL 180

Query: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           FDNFEKLEDAAKQKNLSETE+YYQQTTPILQEVMDRMA
Sbjct: 181 FDNFEKLEDAAKQKNLSETEAYYQQTTPILQEVMDRMA 218

[Close]

BLAST of MS021375 vs. NCBI nr
Match: XP_022981605.1 (photosynthetic NDH subunit of lumenal location 3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 390.6 bits (1002), Expect = 9.0e-105
Identity = 198/218 (90.83%), Postives = 205/218 (94.04%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60
           MARLANLNGVS+KFSAIPKL KL+  RKR KI+G L KQ EVSSH  S TRRLALTS+ S
Sbjct: 1   MARLANLNGVSEKFSAIPKLSKLEKTRKRSKIMGLLEKQAEVSSHRISLTRRLALTSLAS 60

Query: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120
           VALFGNASVEIASANEYWLDGPLP+PSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR
Sbjct: 61  VALFGNASVEIASANEYWLDGPLPIPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120

Query: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRL 180
           LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAA DGEK+PLT LANRL
Sbjct: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAEDGEKQPLTNLANRL 180

Query: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           FDNFEKLEDAAKQKNLSETE+YYQQTTPILQEVMDRMA
Sbjct: 181 FDNFEKLEDAAKQKNLSETEAYYQQTTPILQEVMDRMA 218

[Close]

BLAST of MS021375 vs. NCBI nr
Match: KAG7015220.1 (Photosynthetic NDH subunit of lumenal location 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 389.8 bits (1000), Expect = 1.5e-104
Identity = 198/218 (90.83%), Postives = 204/218 (93.58%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60
           MARLANLNGVS+KFSAIPKL KLQ  RKR KI G L KQ EVSSH  S TRRLALTS+ S
Sbjct: 1   MARLANLNGVSEKFSAIPKLSKLQKTRKRSKITGLLEKQVEVSSHEISLTRRLALTSLAS 60

Query: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120
           VALFGNA VEIASANEYWLDGPLP+PSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR
Sbjct: 61  VALFGNAPVEIASANEYWLDGPLPIPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120

Query: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRL 180
           LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAA DGEK+PLT+LANRL
Sbjct: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAEDGEKQPLTDLANRL 180

Query: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           FDNFEKLEDAAKQKNLSETE+YYQQTTPILQEVMDRMA
Sbjct: 181 FDNFEKLEDAAKQKNLSETEAYYQQTTPILQEVMDRMA 218

[Close]

BLAST of MS021375 vs. NCBI nr
Match: KAG6607822.1 (Photosynthetic NDH subunit of lumenal location 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 387.5 bits (994), Expect = 7.6e-104
Identity = 197/218 (90.37%), Postives = 203/218 (93.12%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60
           MARLANLNGVS+KFSAIPKL KLQ  RKR KI G L KQ EVSSH  S TRRLALTS+ S
Sbjct: 1   MARLANLNGVSEKFSAIPKLSKLQKTRKRSKITGLLEKQVEVSSHEISLTRRLALTSLAS 60

Query: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120
           VALFGNA VEIASANEYWLDGPLP+PSVYNNIANEKTGTRSFLKTGIYIA IGTEGRKYR
Sbjct: 61  VALFGNAPVEIASANEYWLDGPLPIPSVYNNIANEKTGTRSFLKTGIYIAKIGTEGRKYR 120

Query: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRL 180
           LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAA DGEK+PLT+LANRL
Sbjct: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAEDGEKQPLTDLANRL 180

Query: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           FDNFEKLEDAAKQKNLSETE+YYQQTTPILQEVMDRMA
Sbjct: 181 FDNFEKLEDAAKQKNLSETEAYYQQTTPILQEVMDRMA 218

[Close]

BLAST of MS021375 vs. ExPASy Swiss-Prot
Match: Q9SGH4 (Photosynthetic NDH subunit of lumenal location 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PNSL3 PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 3.5e-67
Identity = 133/223 (59.64%), Postives = 166/223 (74.44%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSS--FTRRLALTSI 60
           MA   +LN ++   + +P LPKL   RK  K  GF  ++TE      S   TRR+ L   
Sbjct: 1   MAHFIDLNSLT---NTLPSLPKLPESRKTGKSSGFACRRTEEFQEPDSVQITRRMTLGFA 60

Query: 61  VSVAL---FGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTE 120
           VS+ L    G  +V +A  N +W+DGPLP+P +YNNI NEKTGTR+F+K G+Y+A+IGT+
Sbjct: 61  VSIGLTGILGENNVSLAQDNGFWIDGPLPIPPIYNNIVNEKTGTRTFIKKGVYVADIGTK 120

Query: 121 GRKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTE 180
           GR YR+K+ AFDLLAM DLIG DTLNYV+KYLRLKSTF++YDFDN+ISAAA  +K+PLT+
Sbjct: 121 GRMYRVKKNAFDLLAMEDLIGPDTLNYVKKYLRLKSTFLFYDFDNLISAAASEDKQPLTD 180

Query: 181 LANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           LANRLFDNFEKLEDAAK KNL+ETES Y+ T  +LQEVM RMA
Sbjct: 181 LANRLFDNFEKLEDAAKTKNLAETESCYKDTKFLLQEVMTRMA 220

[Close]

BLAST of MS021375 vs. ExPASy Swiss-Prot
Match: Q9XFT3 (Oxygen-evolving enhancer protein 3-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSBQ1 PE=1 SV=3)

HSP 1 Score: 79.7 bits (195), Expect = 4.5e-14
Identity = 54/181 (29.83%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 42  VSSHHSSFTRRLALTSIVSVALFGNASVEIASANEYWLD-GPLPVPSVYNNIANEKTG-T 101
           V +  S  T R ++  +V+  L G + V+   A+   +  GP P PS       + +   
Sbjct: 43  VRAQQSEETSRRSVIGLVAAGLAGGSFVQAVLADAISIKVGPPPAPSGGLPAGTDNSDQA 102

Query: 102 RSF---LKTGIYIANIGTEGRKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYY 161
           R F   LK   Y+  +       R K  A D++ +  LI +    YV+  LR K++++ Y
Sbjct: 103 RDFALALKDRFYLQPLPPTEAAARAKESAKDIINVKPLIDRKAWPYVQNDLRSKASYLRY 162

Query: 162 DFDNVISAAADGEKEPLTELANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDR 218
           D + +IS+    EK+ L +L  +LFD  + L+ AAK+K+ S+ E YY +T   L EV+ +
Sbjct: 163 DLNTIISSKPKDEKKSLKDLTTKLFDTIDNLDYAAKKKSPSQAEKYYAETVSALNEVLAK 222

[Close]

BLAST of MS021375 vs. ExPASy Swiss-Prot
Match: P12301 (Oxygen-evolving enhancer protein 3, chloroplastic OS=Spinacia oleracea OX=3562 GN=PSBQ PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.3e-12
Identity = 61/229 (26.64%), Postives = 104/229 (45.41%), Query Frame = 0


Query: 1   MARLANLNGVSD-------KFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRL 60
           MA +A L G S        + S   +L      R  +  +G   +  +VS+   + T R 
Sbjct: 5   MASMAGLRGASQAVLEGSLQISGSNRLSGPTTSRVAVPKMGLNIRAQQVSA--EAETSRR 64

Query: 61  ALTSIVSVALFGNASVE--IASANEYWLDGPLPVPSVYNNIANE---KTGTRSFLKTGIY 120
           A+   V+  L   + V+  +A A    +  P P+        N    + GT  + K   Y
Sbjct: 65  AMLGFVAAGLASGSFVKAVLAEARPIVVGPPPPLSGGLPGTENSDQARDGTLPYTKDRFY 124

Query: 121 IANIGTEGRKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADG 180
           +  +       R K  A ++L +   I +     ++  LRL+++++ YD   VISA    
Sbjct: 125 LQPLPPTEAAQRAKVSASEILNVKQFIDRKAWPSLQNDLRLRASYLRYDLKTVISAKPKD 184

Query: 181 EKEPLTELANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRM 218
           EK+ L EL ++LF + + L+ AAK K+ +E E YY QT   + EV+ ++
Sbjct: 185 EKKSLQELTSKLFSSIDNLDHAAKIKSPTEAEKYYGQTVSNINEVLAKL 231

[Close]

BLAST of MS021375 vs. ExPASy Swiss-Prot
Match: Q9XI73 (Photosynthetic NDH subunit of lumenal location 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PNSL2 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.7e-11
Identity = 45/170 (26.47%), Postives = 82/170 (48.24%), Query Frame = 0


Query: 49  FTRRLALTSIVSVALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIY 108
           FTRR  LTS+++  + G A+   +SA                 +A EK GTRSF+K   +
Sbjct: 41  FTRRRTLTSLITFTVIGGAT---SSA-----------------LAQEKWGTRSFIKEKYF 100

Query: 109 IANIGTEGRKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADG 168
           +  +  E    R+K+ A  L  M +++   +  YV  Y+RLK  ++  D  N ++   + 
Sbjct: 101 MPGLSPEDAAARIKQTAEGLRDMREMLDHMSWRYVIFYIRLKQAYLSQDLTNAMNILPES 160

Query: 169 EKEPLTELANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
            +    + AN L +N  +L+   +   + E+  YY++T   +  V++ +A
Sbjct: 161 RRNDYVQAANELVENMSELDFYVRTPKVYESYLYYEKTLKSIDNVVEFLA 190

[Close]

BLAST of MS021375 vs. ExPASy Swiss-Prot
Match: P83646 (Oxygen-evolving enhancer protein 3, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_025465 PE=1 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 3.5e-11
Identity = 59/221 (26.70%), Postives = 102/221 (46.15%), Query Frame = 0


Query: 4   LANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVSVAL 63
           +A++ G+S     +P  P+    R RL ++       +V       T R A+  +V+  +
Sbjct: 5   MASMTGLSQGVQ-LPAGPRRAGGRSRLAVVRADAAAADVQ------TGRRAVLGLVATGI 64

Query: 64  FGNASVEIA---SANEYWLDGPLP----VPSVYNNIANEKTGTRSFLKTGIYIANIGTEG 123
            G A  + A   +A    L  P P    +P   N  +++   T   L+   Y+  +    
Sbjct: 65  AGGALAQAALAEAAKPIKLGPPPPPSGGLPGTLN--SDQARDTDLPLRERFYLQPLPPAE 124

Query: 124 RKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTEL 183
              R K  A D++ +  LI K    +VR  LRL+++++ YD   VI++    EK+ L +L
Sbjct: 125 AAARAKESAQDIINLKPLIEKKQWPFVRDDLRLRASYLRYDLKTVINSKPKDEKKGLKDL 184

Query: 184 ANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRM 218
             +LF   + L+ AAK K+  E E YY  T   L +V+ ++
Sbjct: 185 TGKLFATIDGLDHAAKIKSPEEAEKYYTLTKSALGDVLAKL 216

[Close]

BLAST of MS021375 vs. ExPASy TrEMBL
Match: A0A6J1CCI9 (photosynthetic NDH subunit of lumenal location 3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111010414 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 2.5e-116
Identity = 217/218 (99.54%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60
           MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS
Sbjct: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60

Query: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120
           VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR
Sbjct: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120

Query: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRL 180
           LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEK+PLTELANRL
Sbjct: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKQPLTELANRL 180

Query: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA
Sbjct: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 218

[Close]

BLAST of MS021375 vs. ExPASy TrEMBL
Match: A0A6J1FHS2 (photosynthetic NDH subunit of lumenal location 3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445892 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.1e-105
Identity = 199/218 (91.28%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60
           MARLANLNGVS+KFSAIPKL KLQ  RKR KI+G L KQ EVSSH  S TRRLALTS+ S
Sbjct: 1   MARLANLNGVSEKFSAIPKLSKLQKTRKRSKIMGLLEKQAEVSSHEVSLTRRLALTSLTS 60

Query: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120
           VALFGNASVEIASANEYWLDGPLP+PSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR
Sbjct: 61  VALFGNASVEIASANEYWLDGPLPIPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120

Query: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRL 180
           LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAA DGEK+PLT+LANRL
Sbjct: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAEDGEKQPLTDLANRL 180

Query: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           FDNFEKLEDAAKQKNLSETE+YYQQTTPILQEVMDRMA
Sbjct: 181 FDNFEKLEDAAKQKNLSETEAYYQQTTPILQEVMDRMA 218

[Close]

BLAST of MS021375 vs. ExPASy TrEMBL
Match: A0A6J1IZZ5 (photosynthetic NDH subunit of lumenal location 3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111480674 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.4e-105
Identity = 198/218 (90.83%), Postives = 205/218 (94.04%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60
           MARLANLNGVS+KFSAIPKL KL+  RKR KI+G L KQ EVSSH  S TRRLALTS+ S
Sbjct: 1   MARLANLNGVSEKFSAIPKLSKLEKTRKRSKIMGLLEKQAEVSSHRISLTRRLALTSLAS 60

Query: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120
           VALFGNASVEIASANEYWLDGPLP+PSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR
Sbjct: 61  VALFGNASVEIASANEYWLDGPLPIPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120

Query: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRL 180
           LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAA DGEK+PLT LANRL
Sbjct: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAEDGEKQPLTNLANRL 180

Query: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           FDNFEKLEDAAKQKNLSETE+YYQQTTPILQEVMDRMA
Sbjct: 181 FDNFEKLEDAAKQKNLSETEAYYQQTTPILQEVMDRMA 218

[Close]

BLAST of MS021375 vs. ExPASy TrEMBL
Match: A0A0A0L7N1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G414060 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.2e-100
Identity = 194/218 (88.99%), Postives = 201/218 (92.20%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60
           MARLANLNG   KFSAIP LPKLQN  KRLKI GFL KQT+VSSH  S TRRLALTS+VS
Sbjct: 1   MARLANLNG---KFSAIPNLPKLQNIHKRLKITGFLKKQTDVSSHQISLTRRLALTSLVS 60

Query: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120
           VALFGNAS EIASANEYWLDGPLPVPSV NNI NE+TGTRSFLKTGIYIANIGT+GRKYR
Sbjct: 61  VALFGNASPEIASANEYWLDGPLPVPSVDNNIVNEQTGTRSFLKTGIYIANIGTKGRKYR 120

Query: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRL 180
           LK+YAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFD VISAAAD EK+PL +LANRL
Sbjct: 121 LKKYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDKVISAAADDEKQPLVDLANRL 180

Query: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA
Sbjct: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 215

[Close]

BLAST of MS021375 vs. ExPASy TrEMBL
Match: A0A5A7TX10 (Photosynthetic NDH subunit of lumenal location 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1251G00750 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 9.4e-100
Identity = 193/218 (88.53%), Postives = 202/218 (92.66%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSSFTRRLALTSIVS 60
           MARLANLNG   KFSAIP LPKLQN  K+LKI GFL KQT+VSSH  S TRRLALTS+VS
Sbjct: 1   MARLANLNG---KFSAIPNLPKLQNIHKKLKITGFLKKQTDVSSHQISLTRRLALTSLVS 60

Query: 61  VALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTEGRKYR 120
           VALFGNAS EIASANEYWLDGPLPVPSV NNIANE+TGTRSFLKTGIYIANIGT+GRKYR
Sbjct: 61  VALFGNASPEIASANEYWLDGPLPVPSVDNNIANEQTGTRSFLKTGIYIANIGTKGRKYR 120

Query: 121 LKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTELANRL 180
           LK+YAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFD VISAAAD EK+PL +LANRL
Sbjct: 121 LKKYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDKVISAAADDEKQPLVDLANRL 180

Query: 181 FDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           FDNFEKLE+AAKQKNLSETESYYQQTTPILQEVMDRMA
Sbjct: 181 FDNFEKLENAAKQKNLSETESYYQQTTPILQEVMDRMA 215

[Close]

BLAST of MS021375 vs. TAIR 10
Match: AT3G01440.1 (PsbQ-like 1 )

HSP 1 Score: 256.1 bits (653), Expect = 2.5e-68
Identity = 133/223 (59.64%), Postives = 166/223 (74.44%), Query Frame = 0


Query: 1   MARLANLNGVSDKFSAIPKLPKLQNPRKRLKIIGFLGKQTEVSSHHSS--FTRRLALTSI 60
           MA   +LN ++   + +P LPKL   RK  K  GF  ++TE      S   TRR+ L   
Sbjct: 1   MAHFIDLNSLT---NTLPSLPKLPESRKTGKSSGFACRRTEEFQEPDSVQITRRMTLGFA 60

Query: 61  VSVAL---FGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIYIANIGTE 120
           VS+ L    G  +V +A  N +W+DGPLP+P +YNNI NEKTGTR+F+K G+Y+A+IGT+
Sbjct: 61  VSIGLTGILGENNVSLAQDNGFWIDGPLPIPPIYNNIVNEKTGTRTFIKKGVYVADIGTK 120

Query: 121 GRKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADGEKEPLTE 180
           GR YR+K+ AFDLLAM DLIG DTLNYV+KYLRLKSTF++YDFDN+ISAAA  +K+PLT+
Sbjct: 121 GRMYRVKKNAFDLLAMEDLIGPDTLNYVKKYLRLKSTFLFYDFDNLISAAASEDKQPLTD 180

Query: 181 LANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
           LANRLFDNFEKLEDAAK KNL+ETES Y+ T  +LQEVM RMA
Sbjct: 181 LANRLFDNFEKLEDAAKTKNLAETESCYKDTKFLLQEVMTRMA 220

[Close]

BLAST of MS021375 vs. TAIR 10
Match: AT4G21280.1 (photosystem II subunit QA )

HSP 1 Score: 81.6 bits (200), Expect = 8.3e-16
Identity = 54/180 (30.00%), Postives = 90/180 (50.00%), Query Frame = 0


Query: 42  VSSHHSSFTRRLALTSIVSVALFGNASVEIASANEYWLD-GPLPVPSVYNNIANEKTGTR 101
           V +  S  T R ++  +V+  L G + V+   A+   +  GP P PS      +     R
Sbjct: 43  VRAQQSEETSRRSVIGLVAAGLAGGSFVQAVLADAISIKVGPPPAPSGGLPGTDNSDQAR 102

Query: 102 SF---LKTGIYIANIGTEGRKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYD 161
            F   LK   Y+  +       R K  A D++ +  LI +    YV+  LR K++++ YD
Sbjct: 103 DFALALKDRFYLQPLPPTEAAARAKESAKDIINVKPLIDRKAWPYVQNDLRSKASYLRYD 162

Query: 162 FDNVISAAADGEKEPLTELANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRM 218
            + +IS+    EK+ L +L  +LFD  + L+ AAK+K+ S+ E YY +T   L EV+ ++
Sbjct: 163 LNTIISSKPKDEKKSLKDLTTKLFDTIDNLDYAAKKKSPSQAEKYYAETVSALNEVLAKL 222

[Close]

BLAST of MS021375 vs. TAIR 10
Match: AT4G21280.2 (photosystem II subunit QA )

HSP 1 Score: 79.7 bits (195), Expect = 3.2e-15
Identity = 54/181 (29.83%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 42  VSSHHSSFTRRLALTSIVSVALFGNASVEIASANEYWLD-GPLPVPSVYNNIANEKTG-T 101
           V +  S  T R ++  +V+  L G + V+   A+   +  GP P PS       + +   
Sbjct: 43  VRAQQSEETSRRSVIGLVAAGLAGGSFVQAVLADAISIKVGPPPAPSGGLPAGTDNSDQA 102

Query: 102 RSF---LKTGIYIANIGTEGRKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYY 161
           R F   LK   Y+  +       R K  A D++ +  LI +    YV+  LR K++++ Y
Sbjct: 103 RDFALALKDRFYLQPLPPTEAAARAKESAKDIINVKPLIDRKAWPYVQNDLRSKASYLRY 162

Query: 162 DFDNVISAAADGEKEPLTELANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDR 218
           D + +IS+    EK+ L +L  +LFD  + L+ AAK+K+ S+ E YY +T   L EV+ +
Sbjct: 163 DLNTIISSKPKDEKKSLKDLTTKLFDTIDNLDYAAKKKSPSQAEKYYAETVSALNEVLAK 222

[Close]

BLAST of MS021375 vs. TAIR 10
Match: AT1G14150.1 (PsbQ-like 2 )

HSP 1 Score: 70.5 bits (171), Expect = 1.9e-12
Identity = 45/170 (26.47%), Postives = 82/170 (48.24%), Query Frame = 0


Query: 49  FTRRLALTSIVSVALFGNASVEIASANEYWLDGPLPVPSVYNNIANEKTGTRSFLKTGIY 108
           FTRR  LTS+++  + G A+   +SA                 +A EK GTRSF+K   +
Sbjct: 41  FTRRRTLTSLITFTVIGGAT---SSA-----------------LAQEKWGTRSFIKEKYF 100

Query: 109 IANIGTEGRKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTFMYYDFDNVISAAADG 168
           +  +  E    R+K+ A  L  M +++   +  YV  Y+RLK  ++  D  N ++   + 
Sbjct: 101 MPGLSPEDAAARIKQTAEGLRDMREMLDHMSWRYVIFYIRLKQAYLSQDLTNAMNILPES 160

Query: 169 EKEPLTELANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEVMDRMA 219
            +    + AN L +N  +L+   +   + E+  YY++T   +  V++ +A
Sbjct: 161 RRNDYVQAANELVENMSELDFYVRTPKVYESYLYYEKTLKSIDNVVEFLA 190

[Close]

BLAST of MS021375 vs. TAIR 10
Match: AT4G05180.1 (photosystem II subunit Q-2 )

HSP 1 Score: 69.7 bits (169), Expect = 3.3e-12
Identity = 55/184 (29.89%), Postives = 88/184 (47.83%), Query Frame = 0


Query: 39  QTEVSSHHSSFTRRLALTSIVSVALFGNASVE--IASANEYWLDGPLPVPSVYNNIANEK 98
           Q  VS   SS   R ++  +V+  L G + V+   A A    + GP P+PS      +  
Sbjct: 50  QQNVSVPESS---RRSVIGLVAAGLAGGSFVKAVFAEAIPIKVGGP-PLPSGGLPGTDNS 109

Query: 99  TGTRSF---LKTGIYIANIGTEGRKYRLKRYAFDLLAMADLIGKDTLNYVRKYLRLKSTF 158
              R F   LK   YI  +       R K  A +++ +   I K    YV+  LRL++++
Sbjct: 110 DQARDFSLALKDRFYIQPLSPTEAAARAKDSAKEIINVKSFIDKKAWPYVQNDLRLRASY 169

Query: 159 MYYDFDNVISAAADGEKEPLTELANRLFDNFEKLEDAAKQKNLSETESYYQQTTPILQEV 218
           + YD + VISA    EK+ L +L  +LF   + L+ AA+ K+  + E YY +T   L  V
Sbjct: 170 LRYDLNTVISAKPKEEKQSLKDLTAKLFQTIDNLDYAARSKSSPDAEKYYSETVSSLNNV 229

The following BLAST results are available for this feature:

BLAST of MS021375 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139520.1	5.1e-116	99.54	photosynthetic NDH subunit of lumenal location 3, chloroplastic [Momordica chara...	[more]
XP_022940191.1	2.4e-105	91.28	photosynthetic NDH subunit of lumenal location 3, chloroplastic [Cucurbita mosch...	[more]
XP_022981605.1	9.0e-105	90.83	photosynthetic NDH subunit of lumenal location 3, chloroplastic [Cucurbita maxim...	[more]
KAG7015220.1	1.5e-104	90.83	Photosynthetic NDH subunit of lumenal location 3, chloroplastic [Cucurbita argyr...	[more]
KAG6607822.1	7.6e-104	90.37	Photosynthetic NDH subunit of lumenal location 3, chloroplastic, partial [Cucurb...	[more]

BLAST of MS021375 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SGH4	3.5e-67	59.64	Photosynthetic NDH subunit of lumenal location 3, chloroplastic OS=Arabidopsis t...	[more]
Q9XFT3	4.5e-14	29.83	Oxygen-evolving enhancer protein 3-1, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
P12301	9.3e-12	26.64	Oxygen-evolving enhancer protein 3, chloroplastic OS=Spinacia oleracea OX=3562 G...	[more]
Q9XI73	2.7e-11	26.47	Photosynthetic NDH subunit of lumenal location 2, chloroplastic OS=Arabidopsis t...	[more]
P83646	3.5e-11	26.70	Oxygen-evolving enhancer protein 3, chloroplastic OS=Oryza sativa subsp. indica ...	[more]

BLAST of MS021375 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CCI9	2.5e-116	99.54	photosynthetic NDH subunit of lumenal location 3, chloroplastic OS=Momordica cha...	[more]
A0A6J1FHS2	1.1e-105	91.28	photosynthetic NDH subunit of lumenal location 3, chloroplastic OS=Cucurbita mos...	[more]
A0A6J1IZZ5	4.4e-105	90.83	photosynthetic NDH subunit of lumenal location 3, chloroplastic OS=Cucurbita max...	[more]
A0A0A0L7N1	3.2e-100	88.99	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G414060 PE=3 SV=1	[more]
A0A5A7TX10	9.4e-100	88.53	Photosynthetic NDH subunit of lumenal location 3 OS=Cucumis melo var. makuwa OX=...	[more]

BLAST of MS021375 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01440.1	2.5e-68	59.64	PsbQ-like 1	[more]
AT4G21280.1	8.3e-16	30.00	photosystem II subunit QA	[more]
AT4G21280.2	3.2e-15	29.83	photosystem II subunit QA	[more]
AT1G14150.1	1.9e-12	26.47	PsbQ-like 2	[more]
AT4G05180.1	3.3e-12	29.89	photosystem II subunit Q-2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008797	Oxygen-evolving enhancer protein 3	PFAM	PF05757	PsbQ	coord: 24..218 e-value: 6.5E-55 score: 186.0
IPR008797	Oxygen-evolving enhancer protein 3	PANTHER	PTHR33399	OXYGEN-EVOLVING ENHANCER PROTEIN 3-1, CHLOROPLASTIC	coord: 1..218
IPR023222	PsbQ-like domain superfamily	GENE3D	1.20.120.290		coord: 80..218 e-value: 6.3E-45 score: 154.8
IPR023222	PsbQ-like domain superfamily	SUPERFAMILY	101112	Oxygen-evolving enhancer protein 3,	coord: 108..218
None	No IPR available	PANTHER	PTHR33399:SF2	PHOTOSYNTHETIC NDH SUBUNIT OF LUMENAL LOCATION 3, CHLOROPLASTIC	coord: 1..218

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021375.1	MS021375.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015979	photosynthesis
cellular_component	GO:0019898	extrinsic component of membrane
cellular_component	GO:0009523	photosystem II
cellular_component	GO:0009654	photosystem II oxygen evolving complex
molecular_function	GO:0005509	calcium ion binding