MS021367 (gene) Bitter gourd (TR) v1

BLAST of MS021367 vs. NCBI nr
Match: XP_022157153.1 (beta-amyrin 11-oxidase-like [Momordica charantia] >BBI21929.1 cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 559.7 bits (1441), Expect = 2.3e-155
Identity = 262/461 (56.83%), Postives = 348/461 (75.49%), Query Frame = 0

Query: 2   ILSNFWGILGAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 61
           +LSNF  ILGA+LGLYILVFGFLRKFN +WY  +L + +Y+ LPPGDMGWPL+GS L+FY
Sbjct: 3   LLSNFGAILGALLGLYILVFGFLRKFNDIWYRFKLGKNVYNALPPGDMGWPLVGSSLSFY 62

Query: 62  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 121
           +A+KIGG+PN VMD+L+SR+G+V MYK HLYG P IIVT+PE CRRIY DDEHFK NYPK
Sbjct: 63  QAFKIGGDPNAVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPK 122

Query: 122 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRR-EIEL 181
           +V +L  DGSL +++ KI+HR +A PING E LSRYVEFIE  +VE LEEW S +  + L
Sbjct: 123 SVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPL 182

Query: 182 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 241
           +HE + LTFKII  VF+G   T  + +E+  LYA+I P IMSIF Y+LPG ++R+A+KAR
Sbjct: 183 LHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKAR 242

Query: 242 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 301
            ++ NI+ PVI  R++ +E N ++EE  Q+D  +KA D NGE  FN+NA++CMLLGLL+A
Sbjct: 243 IKVENIVRPVIQNRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYA 302

Query: 302 GRDTSAHAAMWLLIYVLQHPQVYQKAK-----VVNESIPNFSKIQFKLDFSFINLLFQVI 361
           G  TSAH A W +++V ++P VYQKAK     ++ +  PN   + FK +   +    ++I
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPNQKGLNFK-EIKQMKYTAKII 362

Query: 362 NETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWD 421
           NE LR++++   + R AT D+N++GYTIPK W   + +R +HMD +IY NP +F+PSRWD
Sbjct: 363 NEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWD 422

Query: 422 NYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           NYTPKPG ++ FG+G RFCPGNELA+LEMT+LLHHFLL Y+
Sbjct: 423 NYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 462

BLAST of MS021367 vs. NCBI nr
Match: BBI21930.1 (cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 555.4 bits (1430), Expect = 4.4e-154
Identity = 267/461 (57.92%), Postives = 349/461 (75.70%), Query Frame = 0

Query: 2   ILSNFWGILGAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 61
           IL+NFW +LGA +GLY  VFGFLR FN LWYFLRL  KIY+ LPPG MGWPL+GS L+FY
Sbjct: 3   ILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFY 62

Query: 62  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 121
           KA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+Y DDE FK +YPK
Sbjct: 63  KAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPK 122

Query: 122 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSS-RREIEL 181
           +VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ LEEW S +  +EL
Sbjct: 123 SVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVEL 182

Query: 182 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 241
           + E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YDLPG ++R+A+KAR
Sbjct: 183 LLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKAR 242

Query: 242 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 301
            ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+NA++CMLLGLL+A
Sbjct: 243 SKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIICMLLGLLYA 302

Query: 302 GRDTSAHAAMWLLIYVLQHPQVYQKAKVVNESI----PNFSK-IQFKLDFSFINLLFQVI 361
           G  TSAH A W +++V ++P VYQKAK   E I    P+  K + FK +   +    +VI
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK-EIKQMKYATKVI 362

Query: 362 NETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWD 421
           NE LR++++   + R ATTD+N++GY IPK W V I  R +HMD +IY NP +FDPSRWD
Sbjct: 363 NEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWD 422

Query: 422 NYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           NY PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+
Sbjct: 423 NYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

BLAST of MS021367 vs. NCBI nr
Match: XP_022157134.1 (beta-amyrin 11-oxidase-like isoform X1 [Momordica charantia])

HSP 1 Score: 553.9 bits (1426), Expect = 1.3e-153
Identity = 267/461 (57.92%), Postives = 348/461 (75.49%), Query Frame = 0

Query: 2   ILSNFWGILGAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 61
           IL+NFW +LGA +GLY  VFGFLR FN LWYFLRL  KIY+ LPPG MGWPL+GS L+FY
Sbjct: 3   ILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFY 62

Query: 62  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 121
           KA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+Y DDE FK +YPK
Sbjct: 63  KAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPK 122

Query: 122 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSS-RREIEL 181
           +VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ LEEW S +  +EL
Sbjct: 123 SVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVEL 182

Query: 182 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 241
           + E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YDLPG ++R+A+KAR
Sbjct: 183 LLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKAR 242

Query: 242 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 301
            ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+NA+ CMLLGLL+A
Sbjct: 243 SKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYA 302

Query: 302 GRDTSAHAAMWLLIYVLQHPQVYQKAKVVNESI----PNFSK-IQFKLDFSFINLLFQVI 361
           G  TSAH A W +++V ++P VYQKAK   E I    P+  K + FK +   +    +VI
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK-EIKQMKYATKVI 362

Query: 362 NETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWD 421
           NE LR++++   + R ATTD+N++GY IPK W V I  R +HMD +IY NP +FDPSRWD
Sbjct: 363 NEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWD 422

Query: 422 NYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           NY PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+
Sbjct: 423 NYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

BLAST of MS021367 vs. NCBI nr
Match: XP_022157141.1 (beta-amyrin 11-oxidase-like isoform X2 [Momordica charantia])

HSP 1 Score: 485.0 bits (1247), Expect = 7.3e-133
Identity = 234/414 (56.52%), Postives = 311/414 (75.12%), Query Frame = 0

Query: 49  MGWPLIGSLLTFYKAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRI 108
           MGWPL+GS L+FYKA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 109 YSDDEHFKFNYPKTVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEA 168
           Y DDE FK +YPK+VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ 
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 169 LEEWSS-RREIELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYD 228
           LEEW S +  +EL+ E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YD
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 229 LPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNN 288
           LPG ++R+A+KAR ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+
Sbjct: 181 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 240

Query: 289 NAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKVVNESI----PNFSK-IQFK 348
           NA+ CMLLGLL+AG  TSAH A W +++V ++P VYQKAK   E I    P+  K + FK
Sbjct: 241 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 300

Query: 349 LDFSFINLLFQVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQI 408
            +   +    +VINE LR++++   + R ATTD+N++GY IPK W V I  R +HMD +I
Sbjct: 301 -EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEI 360

Query: 409 YPNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           Y NP +FDPSRWDNY PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+
Sbjct: 361 YQNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 413

BLAST of MS021367 vs. NCBI nr
Match: KAG6571834.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 473.4 bits (1217), Expect = 2.2e-129
Identity = 233/457 (50.98%), Postives = 321/457 (70.24%), Query Frame = 0

Query: 5   NFWGILGAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAY 64
           N   I+GAVLG+Y+    F+RK N +WY ++L RK Y +LPPGDMGWPL+GS  +FYKA+
Sbjct: 5   NVCTIIGAVLGVYM----FVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 64

Query: 65  KIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVN 124
            +GG+P+  +  L+SR G+VGMYK HLYG PTIIVT+PE CRRIY DDE FK NYPK+V 
Sbjct: 65  TVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 124

Query: 125 ILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRRE-IELVHE 184
           IL  +G  TR + K  +R +A P+NG E LS YV+FI++++ + LEEWSS +E IEL+ E
Sbjct: 125 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 184

Query: 185 LRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEI 244
           +  L FK+I+ +F+GT +   + +E+  LY  + P I+SI  YD+PG  + +A+KAR +I
Sbjct: 185 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKARKKI 244

Query: 245 LNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRD 304
            N+L  VI E+RR +EK E  E   Q+D  + A+D NG   ++NN ++ +LLGLL AG  
Sbjct: 245 ENVLECVIEEKRRALEKKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHAGHH 304

Query: 305 TSAHAAMWLLIYVLQHPQVYQKAKVVNESI----PNFSKIQFKLDFSFINLLFQVINETL 364
           T A+AAMW L+++ + P ++ KAK   ESI    P+  K     +   +  L + INE L
Sbjct: 305 TPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINELL 364

Query: 365 RVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDNYTP 424
           R + +   S RKA TDINI+ Y IPK W+V+I  R +HMD Q+Y NP++FDPSRW+N++P
Sbjct: 365 RRNTIAVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWENHSP 424

Query: 425 KPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           KPGTF+ FG+G RFCPG+EL +LE+TILLHHF+LNYK
Sbjct: 425 KPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYK 457

BLAST of MS021367 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.3e-95
Identity = 190/462 (41.13%), Postives = 291/462 (62.99%), Query Frame = 0

Query: 7   WGILGAVLGLYILVFG--FLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAY 66
           W  + A   L   +FG  F+R  N  +Y ++L RK +  LPPGDMGWPLIG LL+F K +
Sbjct: 5   WVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEH-PLPPGDMGWPLIGDLLSFIKDF 64

Query: 67  KIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVN 126
              G+P+  +++LV +YG+ G+YK HL+G P+IIV  P+ CRR+ +DD +FK  YPK++ 
Sbjct: 65  S-SGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIK 124

Query: 127 ILLGDG---SLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSS-RREIEL 186
            L        ++    ++  R I  PI G + L+ Y+E +E++++ +LEE SS +  +EL
Sbjct: 125 ELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVEL 184

Query: 187 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 246
           + E++ ++FK I HVF+G++       +IG  +  +   + SI   ++PG  F +A++AR
Sbjct: 185 LKEMKKVSFKAIVHVFMGSSNQDI-IKKIGSSFTDLYNGMFSI-PINVPGFTFHKALEAR 244

Query: 247 DEILNILGPVIAERRRVIEK--NEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLL 306
            ++  I+ PV+ ERR +IE    E  + K  +D+ L+ KD NG     +  +  +L+GLL
Sbjct: 245 KKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGR-KLEDEDISDLLIGLL 304

Query: 307 FAGRDTSAHAAMWLLIYVLQHPQVYQKAKVVNESIPN---FSKIQFKL-DFSFINLLFQV 366
           FAG +++A + MW + Y+ QHP + +KAK   E I      S+ Q  L +   +  L QV
Sbjct: 305 FAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQV 364

Query: 367 INETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRW 426
           I+ETLR +++   + R+AT D+NI+GY IPK W V+I  R IHMDS+ YPNP++F+PSRW
Sbjct: 365 IDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRW 424

Query: 427 DNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           D+Y  K GTFL FG G R CPG +LA+LE++I LH+FL NY+
Sbjct: 425 DDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYR 461

BLAST of MS021367 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 334.7 bits (857), Expect = 1.6e-90
Identity = 176/453 (38.85%), Postives = 278/453 (61.37%), Query Frame = 0

Query: 13  VLGLYILVFGFLRKFNHLWYFLRL-ERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPN 72
           +LGL++L +  L++ N   Y  +L E+K Y  LPPGD+GWP+IG++ +F +A+K   +P 
Sbjct: 15  ILGLFVLKW-VLKRVNVWIYVSKLGEKKHY--LPPGDLGWPVIGNMWSFLRAFKT-SDPE 74

Query: 73  YVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGS 132
             +   ++RYG+ G+YK H++G P ++VT PE CRR+ +DD+ F   +PK+   L+G  S
Sbjct: 75  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 134

Query: 133 LTRLN---PKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLT 192
              ++    K L R  + P+NG E LS Y++FIE+ +   LE+WS   EIE +  LR LT
Sbjct: 135 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLT 194

Query: 193 FKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILG 252
           FK+I ++F+ +   H   S + + Y  ++  + ++   +LPG A+ +A+KAR +++    
Sbjct: 195 FKVIMYIFLSSESEHVMDS-LEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQ 254

Query: 253 PVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHA 312
            ++  RR   ++N     K  +D  +  KD NG    ++  ++ +LL  L AG ++S H 
Sbjct: 255 SIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRV-LDDEEIIDLLLMYLNAGHESSGHL 314

Query: 313 AMWLLIYVLQHPQVYQKAK-----VVNESIPNFSKIQFKLDFSFINLLFQVINETLRVSH 372
            MW  I + +HP + QKAK     +V +  P   K+  K     +  L QVI+ETLRV  
Sbjct: 315 TMWATILMQEHPMILQKAKEEQERIVKKRAPG-QKLTLKETREMV-YLSQVIDETLRVIT 374

Query: 373 VTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDNYTPKPGT 432
            +  + R+A +D+ + GY IPK W V+   R++H+D +IYP+P++FDPSRW+ YTPK GT
Sbjct: 375 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGT 434

Query: 433 FLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           FL FG+G   CPGN+LA+LE++I LHHFLL Y+
Sbjct: 435 FLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MS021367 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.9e-84
Identity = 166/452 (36.73%), Postives = 274/452 (60.62%), Query Frame = 0

Query: 13  VLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPNY 72
           VLG + L +  +RK N +W +     +    LPPGD+GWP IG++L+F +A+K   +P+ 
Sbjct: 16  VLGCFGLNW-LVRKVN-VWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKT-SDPDS 75

Query: 73  VMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGSL 132
               L+ RYG  G+YK H++G P+IIVT  + CRR+ +DD+ FK  +P +   L+G  S 
Sbjct: 76  FTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSF 135

Query: 133 TRLN---PKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLTF 192
             ++    K L R  A P+NG E LS Y+ +IE+ ++  L++W+   E E +  LR LTF
Sbjct: 136 VGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTF 195

Query: 193 KIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILGP 252
           +II ++F+ +   +   + + + Y  ++  + ++ + ++PG A+ +A+KAR  ++     
Sbjct: 196 RIIMYIFLSSESENVMDA-LEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQS 255

Query: 253 VIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHAA 312
           ++ ERR   ++N    +K  +D  L  KD +G+ + ++  ++ +LL  L AG ++S H  
Sbjct: 256 IVTERRNQRKQNILSNKKDMLDNLLNVKDEDGK-TLDDEEIIDVLLMYLNAGHESSGHTI 315

Query: 313 MWLLIYVLQHPQVYQKAKVVNESI----PNFSKIQFKLDFSFINLLFQVINETLRVSHVT 372
           MW  +++ +HP+V Q+AK   E I    P   K     +   +  L QV++ETLRV   +
Sbjct: 316 MWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFS 375

Query: 373 TVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDN-YTPKPGTF 432
             + R+A TD+ ++GY IPK W V+   RD+H+D +++P+P++FDP+RWDN + PK G F
Sbjct: 376 LTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAF 435

Query: 433 LSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           L FG G   CPGN+LA+LE++I LHHFLL Y+
Sbjct: 436 LPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQ 461

BLAST of MS021367 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 5.5e-83
Identity = 168/464 (36.21%), Postives = 263/464 (56.68%), Query Frame = 0

Query: 6   FWGILGAVLGLYILVFGFLRKFNHLWYF-LRLERKIYDTLPPGDMGWPLIGSLLTFYKAY 65
           +W +   V  L ++      +  H WY+   L       LPPGDMGWPL+G +  F +A+
Sbjct: 6   WWAVAAVVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAF 65

Query: 66  KIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVN 125
           K  G P+  +D    R+G+ G+Y+  ++ +PTI+ T PEAC+++  DD+ F   +PK   
Sbjct: 66  K-SGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATV 125

Query: 126 ILLGDGSLTRL---NPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSR---REI 185
            L+G  S   +     + L +  A PING + L+ Y+ FI+  +V  L  WS R      
Sbjct: 126 ALIGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHF 185

Query: 186 ELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVK 245
           E + ELR +TF+II  +F+G A      +E+ + Y +++   M   + DLPG A+ +A++
Sbjct: 186 EFLTELRRMTFRIIVQIFMGGA-DERTAAELERTYTELN-YGMRAMAIDLPGFAYHKAIR 245

Query: 246 ARDEILNILGPVIAERRRVIEKNE--EMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLG 305
           AR  ++  L  V+ ERR    K           +D  +  +D  G     ++ ++ +L+ 
Sbjct: 246 ARRRLVAALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGR-RLQDDEIIDVLVM 305

Query: 306 LLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKVVNE----SIPNFSKIQFKLDFSFINLLF 365
            L AG ++S H  MW  +++ ++P++  KAK   E    SIP   K     DF  +  L 
Sbjct: 306 YLNAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLS 365

Query: 366 QVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPS 425
           QV++ETLR  +++ VS R+AT D+ ++GY IPK W V +  R +HMD Q+YP+P++FDPS
Sbjct: 366 QVVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPS 425

Query: 426 RWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           RW+   P+ GTFL FG+G R CPGN+LA+LE+++ LHHFLL YK
Sbjct: 426 RWEGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYK 465

BLAST of MS021367 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 7.2e-83
Identity = 164/462 (35.50%), Postives = 271/462 (58.66%), Query Frame = 0

Query: 5   NFWGILGAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAY 64
           + W +  AV+   +LV   +R+ +       L  +    LPPG+MGWP++GS+  F +A+
Sbjct: 16  DLWVLAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAF 75

Query: 65  KIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVN 124
           K  GNP+  +   + R+G+ G+Y+  ++ +PTI+   PEAC+++  DDE F   +PK   
Sbjct: 76  K-SGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATV 135

Query: 125 ILLGDGSLTRL---NPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRR--EIE 184
            L+G  S   +   + + + +  A PING + L+ Y+ FI++ +V +L  WSS    ++E
Sbjct: 136 TLIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVE 195

Query: 185 LVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKA 244
            + ELR +TFKII  +F+  A        + + Y  ++   M   + +LPG A+ +A++A
Sbjct: 196 FLTELRRMTFKIIVQIFMSGA-DDATMEALERSYTDLN-YGMRAMAINLPGFAYYRALRA 255

Query: 245 RDEILNILGPVIAERRRVIEKNEEMEEKF-QVDVALKAKDANGEFSFNNNAVVCMLLGLL 304
           R +++++L  V+  RR    K  +       +D  ++A+D  G     ++ +V +L+  L
Sbjct: 256 RRKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGR-RLADDEIVDVLIMYL 315

Query: 305 FAGRDTSAHAAMWLLIYVLQHPQVYQKAKVVNE----SIPNFSKIQFKLDFSFINLLFQV 364
            AG ++S H  MW  +++ ++P ++ +AK   E    SIP         DF  ++ L QV
Sbjct: 316 NAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQV 375

Query: 365 INETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRW 424
           ++ETLR  +++ VS R+AT DI ++GY IPK W V +  R +HMD Q+YP+P+ F+PSRW
Sbjct: 376 VDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRW 435

Query: 425 DNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           +   PK GTFL FG+G R CPGN+LA+LE+++ LHHFLL YK
Sbjct: 436 EGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYK 473

BLAST of MS021367 vs. ExPASy TrEMBL
Match: A0A5K7U6A4 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L7 PE=2 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 1.1e-155
Identity = 262/461 (56.83%), Postives = 348/461 (75.49%), Query Frame = 0

Query: 2   ILSNFWGILGAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 61
           +LSNF  ILGA+LGLYILVFGFLRKFN +WY  +L + +Y+ LPPGDMGWPL+GS L+FY
Sbjct: 3   LLSNFGAILGALLGLYILVFGFLRKFNDIWYRFKLGKNVYNALPPGDMGWPLVGSSLSFY 62

Query: 62  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 121
           +A+KIGG+PN VMD+L+SR+G+V MYK HLYG P IIVT+PE CRRIY DDEHFK NYPK
Sbjct: 63  QAFKIGGDPNAVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPK 122

Query: 122 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRR-EIEL 181
           +V +L  DGSL +++ KI+HR +A PING E LSRYVEFIE  +VE LEEW S +  + L
Sbjct: 123 SVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPL 182

Query: 182 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 241
           +HE + LTFKII  VF+G   T  + +E+  LYA+I P IMSIF Y+LPG ++R+A+KAR
Sbjct: 183 LHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKAR 242

Query: 242 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 301
            ++ NI+ PVI  R++ +E N ++EE  Q+D  +KA D NGE  FN+NA++CMLLGLL+A
Sbjct: 243 IKVENIVRPVIQNRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYA 302

Query: 302 GRDTSAHAAMWLLIYVLQHPQVYQKAK-----VVNESIPNFSKIQFKLDFSFINLLFQVI 361
           G  TSAH A W +++V ++P VYQKAK     ++ +  PN   + FK +   +    ++I
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPNQKGLNFK-EIKQMKYTAKII 362

Query: 362 NETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWD 421
           NE LR++++   + R AT D+N++GYTIPK W   + +R +HMD +IY NP +F+PSRWD
Sbjct: 363 NEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWD 422

Query: 422 NYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           NYTPKPG ++ FG+G RFCPGNELA+LEMT+LLHHFLL Y+
Sbjct: 423 NYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 462

BLAST of MS021367 vs. ExPASy TrEMBL
Match: A0A5K7U6M8 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L8 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 2.1e-154
Identity = 267/461 (57.92%), Postives = 349/461 (75.70%), Query Frame = 0

Query: 2   ILSNFWGILGAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 61
           IL+NFW +LGA +GLY  VFGFLR FN LWYFLRL  KIY+ LPPG MGWPL+GS L+FY
Sbjct: 3   ILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFY 62

Query: 62  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 121
           KA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+Y DDE FK +YPK
Sbjct: 63  KAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPK 122

Query: 122 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSS-RREIEL 181
           +VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ LEEW S +  +EL
Sbjct: 123 SVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVEL 182

Query: 182 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 241
           + E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YDLPG ++R+A+KAR
Sbjct: 183 LLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKAR 242

Query: 242 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 301
            ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+NA++CMLLGLL+A
Sbjct: 243 SKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIICMLLGLLYA 302

Query: 302 GRDTSAHAAMWLLIYVLQHPQVYQKAKVVNESI----PNFSK-IQFKLDFSFINLLFQVI 361
           G  TSAH A W +++V ++P VYQKAK   E I    P+  K + FK +   +    +VI
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK-EIKQMKYATKVI 362

Query: 362 NETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWD 421
           NE LR++++   + R ATTD+N++GY IPK W V I  R +HMD +IY NP +FDPSRWD
Sbjct: 363 NEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWD 422

Query: 422 NYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           NY PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+
Sbjct: 423 NYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

BLAST of MS021367 vs. ExPASy TrEMBL
Match: A0A6J1DVM6 (beta-amyrin 11-oxidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 6.2e-154
Identity = 267/461 (57.92%), Postives = 348/461 (75.49%), Query Frame = 0

Query: 2   ILSNFWGILGAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFY 61
           IL+NFW +LGA +GLY  VFGFLR FN LWYFLRL  KIY+ LPPG MGWPL+GS L+FY
Sbjct: 3   ILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFY 62

Query: 62  KAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPK 121
           KA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+Y DDE FK +YPK
Sbjct: 63  KAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPK 122

Query: 122 TVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSS-RREIEL 181
           +VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ LEEW S +  +EL
Sbjct: 123 SVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVEL 182

Query: 182 VHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKAR 241
           + E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YDLPG ++R+A+KAR
Sbjct: 183 LLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKAR 242

Query: 242 DEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFA 301
            ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+NA+ CMLLGLL+A
Sbjct: 243 SKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYA 302

Query: 302 GRDTSAHAAMWLLIYVLQHPQVYQKAKVVNESI----PNFSK-IQFKLDFSFINLLFQVI 361
           G  TSAH A W +++V ++P VYQKAK   E I    P+  K + FK +   +    +VI
Sbjct: 303 GYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK-EIKQMKYATKVI 362

Query: 362 NETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWD 421
           NE LR++++   + R ATTD+N++GY IPK W V I  R +HMD +IY NP +FDPSRWD
Sbjct: 363 NEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWD 422

Query: 422 NYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           NY PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+
Sbjct: 423 NYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

BLAST of MS021367 vs. ExPASy TrEMBL
Match: A0A6J1DX36 (beta-amyrin 11-oxidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 3.6e-133
Identity = 234/414 (56.52%), Postives = 311/414 (75.12%), Query Frame = 0

Query: 49  MGWPLIGSLLTFYKAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRI 108
           MGWPL+GS L+FYKA+KIGG+PN +++ LVSRYG+V MYK HLYG+P IIVT+PE CRR+
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 109 YSDDEHFKFNYPKTVNILLGDGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEA 168
           Y DDE FK +YPK+VNIL GD SL +++ KI+HR +A PINGSE LS+Y+ FIE+ +V+ 
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 169 LEEWSS-RREIELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYD 228
           LEEW S +  +EL+ E + LTFK+IT VF+G A T  + +++  LYA+I   I+SIF YD
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 229 LPGSAFRQAVKARDEILNILGPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNN 288
           LPG ++R+A+KAR ++ NI+ PVI  RRR++E+NEE+EE  Q+D  +KA D NGE  FN+
Sbjct: 181 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 240

Query: 289 NAVVCMLLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKVVNESI----PNFSK-IQFK 348
           NA+ CMLLGLL+AG  TSAH A W +++V ++P VYQKAK   E I    P+  K + FK
Sbjct: 241 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 300

Query: 349 LDFSFINLLFQVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQI 408
            +   +    +VINE LR++++   + R ATTD+N++GY IPK W V I  R +HMD +I
Sbjct: 301 -EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEI 360

Query: 409 YPNPQQFDPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           Y NP +FDPSRWDNY PKPG F+ FG+G R+CPGNELARLE+ ILLHHFLL Y+
Sbjct: 361 YQNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 413

BLAST of MS021367 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.8e-129
Identity = 238/453 (52.54%), Postives = 318/453 (70.20%), Query Frame = 0

Query: 9   ILGAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGG 68
           I+G VLG+Y+    F+RK N +WY ++L RK Y +LPPGDMGWPL+GS  +FY A+ +GG
Sbjct: 9   IIGFVLGVYM----FVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGG 68

Query: 69  NPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLG 128
           +P+  +  L+SR G+VGMYK H YG PTIIVT+PE CRRIY DDE FK NYPK+V IL  
Sbjct: 69  DPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEV 128

Query: 129 DGSLTRLNPKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRRE-IELVHELRGL 188
           +G  TR + K  +R +A P+NG E LS YV+FI++++ + LEEWSS +E IEL+ E+  L
Sbjct: 129 NGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSL 188

Query: 189 TFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNIL 248
            FK+I+ +F+GT +   + +E+  LY  + P I+SI  YDLPG  F  A+KAR +I NIL
Sbjct: 189 FFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIENIL 248

Query: 249 GPVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAH 308
             VI E+RR +EK EE E + Q+D  + A+D NG   ++NNA++ +LLGLL AG  T A+
Sbjct: 249 QCVIEEKRRALEK-EEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPAY 308

Query: 309 AAMWLLIYVLQHPQVYQKAKVVNESI----PNFSKIQFKLDFSFINLLFQVINETLRVSH 368
           AAMW L+++ + P V+ KAK   ESI    P+  K     +   +  L + INE LR + 
Sbjct: 309 AAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNT 368

Query: 369 VTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDNYTPKPGT 428
           +   S RKA  D+NI+ Y IPK WNV+I  R IHMD Q+Y NPQ+FDPSRW+NY+PKPGT
Sbjct: 369 IAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWENYSPKPGT 428

Query: 429 FLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           FL FG+G RFCPG+EL +LE+TILLHHF+LNYK
Sbjct: 429 FLPFGIGTRFCPGSELTKLEITILLHHFILNYK 456

BLAST of MS021367 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 334.7 bits (857), Expect = 1.1e-91
Identity = 176/453 (38.85%), Postives = 278/453 (61.37%), Query Frame = 0

Query: 13  VLGLYILVFGFLRKFNHLWYFLRL-ERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPN 72
           +LGL++L +  L++ N   Y  +L E+K Y  LPPGD+GWP+IG++ +F +A+K   +P 
Sbjct: 15  ILGLFVLKW-VLKRVNVWIYVSKLGEKKHY--LPPGDLGWPVIGNMWSFLRAFKT-SDPE 74

Query: 73  YVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGS 132
             +   ++RYG+ G+YK H++G P ++VT PE CRR+ +DD+ F   +PK+   L+G  S
Sbjct: 75  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 134

Query: 133 LTRLN---PKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLT 192
              ++    K L R  + P+NG E LS Y++FIE+ +   LE+WS   EIE +  LR LT
Sbjct: 135 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLT 194

Query: 193 FKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILG 252
           FK+I ++F+ +   H   S + + Y  ++  + ++   +LPG A+ +A+KAR +++    
Sbjct: 195 FKVIMYIFLSSESEHVMDS-LEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQ 254

Query: 253 PVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHA 312
            ++  RR   ++N     K  +D  +  KD NG    ++  ++ +LL  L AG ++S H 
Sbjct: 255 SIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRV-LDDEEIIDLLLMYLNAGHESSGHL 314

Query: 313 AMWLLIYVLQHPQVYQKAK-----VVNESIPNFSKIQFKLDFSFINLLFQVINETLRVSH 372
            MW  I + +HP + QKAK     +V +  P   K+  K     +  L QVI+ETLRV  
Sbjct: 315 TMWATILMQEHPMILQKAKEEQERIVKKRAPG-QKLTLKETREMV-YLSQVIDETLRVIT 374

Query: 373 VTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDNYTPKPGT 432
            +  + R+A +D+ + GY IPK W V+   R++H+D +IYP+P++FDPSRW+ YTPK GT
Sbjct: 375 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGT 434

Query: 433 FLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           FL FG+G   CPGN+LA+LE++I LHHFLL Y+
Sbjct: 435 FLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MS021367 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 334.7 bits (857), Expect = 1.1e-91
Identity = 176/453 (38.85%), Postives = 278/453 (61.37%), Query Frame = 0

Query: 13  VLGLYILVFGFLRKFNHLWYFLRL-ERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPN 72
           +LGL++L +  L++ N   Y  +L E+K Y  LPPGD+GWP+IG++ +F +A+K   +P 
Sbjct: 15  ILGLFVLKW-VLKRVNVWIYVSKLGEKKHY--LPPGDLGWPVIGNMWSFLRAFKT-SDPE 74

Query: 73  YVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGS 132
             +   ++RYG+ G+YK H++G P ++VT PE CRR+ +DD+ F   +PK+   L+G  S
Sbjct: 75  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 134

Query: 133 LTRLN---PKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLT 192
              ++    K L R  + P+NG E LS Y++FIE+ +   LE+WS   EIE +  LR LT
Sbjct: 135 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGEIEFLSHLRKLT 194

Query: 193 FKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILG 252
           FK+I ++F+ +   H   S + + Y  ++  + ++   +LPG A+ +A+KAR +++    
Sbjct: 195 FKVIMYIFLSSESEHVMDS-LEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQ 254

Query: 253 PVIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHA 312
            ++  RR   ++N     K  +D  +  KD NG    ++  ++ +LL  L AG ++S H 
Sbjct: 255 SIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRV-LDDEEIIDLLLMYLNAGHESSGHL 314

Query: 313 AMWLLIYVLQHPQVYQKAK-----VVNESIPNFSKIQFKLDFSFINLLFQVINETLRVSH 372
            MW  I + +HP + QKAK     +V +  P   K+  K     +  L QVI+ETLRV  
Sbjct: 315 TMWATILMQEHPMILQKAKEEQERIVKKRAPG-QKLTLKETREMV-YLSQVIDETLRVIT 374

Query: 373 VTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDNYTPKPGT 432
            +  + R+A +D+ + GY IPK W V+   R++H+D +IYP+P++FDPSRW+ YTPK GT
Sbjct: 375 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGT 434

Query: 433 FLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           FL FG+G   CPGN+LA+LE++I LHHFLL Y+
Sbjct: 435 FLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MS021367 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-85
Identity = 166/452 (36.73%), Postives = 274/452 (60.62%), Query Frame = 0

Query: 13  VLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLTFYKAYKIGGNPNY 72
           VLG + L +  +RK N +W +     +    LPPGD+GWP IG++L+F +A+K   +P+ 
Sbjct: 16  VLGCFGLNW-LVRKVN-VWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKT-SDPDS 75

Query: 73  VMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEHFKFNYPKTVNILLGDGSL 132
               L+ RYG  G+YK H++G P+IIVT  + CRR+ +DD+ FK  +P +   L+G  S 
Sbjct: 76  FTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSF 135

Query: 133 TRLN---PKILHRFIAPPINGSEPLSRYVEFIEKLIVEALEEWSSRREIELVHELRGLTF 192
             ++    K L R  A P+NG E LS Y+ +IE+ ++  L++W+   E E +  LR LTF
Sbjct: 136 VGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGEFEFLTHLRKLTF 195

Query: 193 KIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQAVKARDEILNILGP 252
           +II ++F+ +   +   + + + Y  ++  + ++ + ++PG A+ +A+KAR  ++     
Sbjct: 196 RIIMYIFLSSESENVMDA-LEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQS 255

Query: 253 VIAERRRVIEKNEEMEEKFQVDVALKAKDANGEFSFNNNAVVCMLLGLLFAGRDTSAHAA 312
           ++ ERR   ++N    +K  +D  L  KD +G+ + ++  ++ +LL  L AG ++S H  
Sbjct: 256 IVTERRNQRKQNILSNKKDMLDNLLNVKDEDGK-TLDDEEIIDVLLMYLNAGHESSGHTI 315

Query: 313 MWLLIYVLQHPQVYQKAKVVNESI----PNFSKIQFKLDFSFINLLFQVINETLRVSHVT 372
           MW  +++ +HP+V Q+AK   E I    P   K     +   +  L QV++ETLRV   +
Sbjct: 316 MWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFS 375

Query: 373 TVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQFDPSRWDN-YTPKPGTF 432
             + R+A TD+ ++GY IPK W V+   RD+H+D +++P+P++FDP+RWDN + PK G F
Sbjct: 376 LTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAF 435

Query: 433 LSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           L FG G   CPGN+LA+LE++I LHHFLL Y+
Sbjct: 436 LPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQ 461

BLAST of MS021367 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 204.5 bits (519), Expect = 1.8e-52
Identity = 149/467 (31.91%), Postives = 233/467 (49.89%), Query Frame = 0

Query: 4   SNFWGIL----GAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLT 63
           SNF+  L     A++ L I+V   +  F   W+    E+++   LPPG MG P IG  L 
Sbjct: 9   SNFFSSLYADEPALITLTIVVVVVVLLFK--WWLHWKEQRL--RLPPGSMGLPYIGETLR 68

Query: 64  FYKAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEH-FKFN 123
            Y       NPN       ++YG +  +K H+ G P +++++PEA R +     H FK  
Sbjct: 69  LYTE-----NPNSFFATRQNKYGDI--FKTHILGCPCVMISSPEAARMVLVSKAHLFKPT 128

Query: 124 YPKTVNILLGDGSLTRLNPKILHRFIAPPINGS---EPLSRYVEFIEKLIVEALEEWSSR 183
           YP +   ++G  +L   +    H  +   +  S     L   V  IE L+++ L  W+S+
Sbjct: 129 YPPSKERMIGPEALF-FHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQ 188

Query: 184 REIELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQ 243
           + I  +  ++   F +      G          I  LY ++     S+   DLPG+ F +
Sbjct: 189 KSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSM-PLDLPGTLFHK 248

Query: 244 AVKARDEILNILGPVIAERRRVIEKNEE--MEEKFQVDVALKAKDANGEFSFNNNAVVCM 303
           ++KAR E+         E R+VIEK  E   EE   + V L AKD       +++ +   
Sbjct: 249 SMKARIELSE-------ELRKVIEKRRENGREEGGLLGVLLGAKDQKRN-GLSDSQIADN 308

Query: 304 LLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKVVNESIPNFSKIQFKL----DFSFIN 363
           ++G++FA  DT+A    WLL Y+  HP + Q+      SI    K + +     D   + 
Sbjct: 309 IIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMP 368

Query: 364 LLFQVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQF 423
           L  +VI ETLR + V + + R+A  D+   GY IPK W V+ + R IH  S+ +P+P++F
Sbjct: 369 LTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKF 428

Query: 424 DPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           DPSR++   PKP T++ FG G   CPG+ELA+LEM ILLHH   +++
Sbjct: 429 DPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFR 453

BLAST of MS021367 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 204.5 bits (519), Expect = 1.8e-52
Identity = 149/467 (31.91%), Postives = 233/467 (49.89%), Query Frame = 0

Query: 4   SNFWGIL----GAVLGLYILVFGFLRKFNHLWYFLRLERKIYDTLPPGDMGWPLIGSLLT 63
           SNF+  L     A++ L I+V   +  F   W+    E+++   LPPG MG P IG  L 
Sbjct: 9   SNFFSSLYADEPALITLTIVVVVVVLLFK--WWLHWKEQRL--RLPPGSMGLPYIGETLR 68

Query: 64  FYKAYKIGGNPNYVMDDLVSRYGKVGMYKCHLYGTPTIIVTNPEACRRIYSDDEH-FKFN 123
            Y       NPN       ++YG +  +K H+ G P +++++PEA R +     H FK  
Sbjct: 69  LYTE-----NPNSFFATRQNKYGDI--FKTHILGCPCVMISSPEAARMVLVSKAHLFKPT 128

Query: 124 YPKTVNILLGDGSLTRLNPKILHRFIAPPINGS---EPLSRYVEFIEKLIVEALEEWSSR 183
           YP +   ++G  +L   +    H  +   +  S     L   V  IE L+++ L  W+S+
Sbjct: 129 YPPSKERMIGPEALF-FHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQ 188

Query: 184 REIELVHELRGLTFKIITHVFVGTALTHFQTSEIGKLYAKISPVIMSIFSYDLPGSAFRQ 243
           + I  +  ++   F +      G          I  LY ++     S+   DLPG+ F +
Sbjct: 189 KSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSM-PLDLPGTLFHK 248

Query: 244 AVKARDEILNILGPVIAERRRVIEKNEE--MEEKFQVDVALKAKDANGEFSFNNNAVVCM 303
           ++KAR E+         E R+VIEK  E   EE   + V L AKD       +++ +   
Sbjct: 249 SMKARIELSE-------ELRKVIEKRRENGREEGGLLGVLLGAKDQKRN-GLSDSQIADN 308

Query: 304 LLGLLFAGRDTSAHAAMWLLIYVLQHPQVYQKAKVVNESIPNFSKIQFKL----DFSFIN 363
           ++G++FA  DT+A    WLL Y+  HP + Q+      SI    K + +     D   + 
Sbjct: 309 IIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMP 368

Query: 364 LLFQVINETLRVSHVTTVSCRKATTDINIHGYTIPKEWNVIIMVRDIHMDSQIYPNPQQF 423
           L  +VI ETLR + V + + R+A  D+   GY IPK W V+ + R IH  S+ +P+P++F
Sbjct: 369 LTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKF 428

Query: 424 DPSRWDNYTPKPGTFLSFGMGGRFCPGNELARLEMTILLHHFLLNYK 457
           DPSR++   PKP T++ FG G   CPG+ELA+LEM ILLHH   +++
Sbjct: 429 DPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFR 453