MS021353 (gene) Bitter gourd (TR) v1

Overview


Name	MS021353
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Adenine nucleotide alpha hydrolases-like superfamily protein
Location	scaffold358: 1300578 .. 1301345 (+)
RNA-Seq Expression	MS021353
Synteny	MS021353

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCTCTGCGGCAGCTTCGAAATTCCTCCTTAGAACCGGACCCGCAGCTGCCATCCATCAAAATTCACCATCCAGCCTCTCCTCGCCACCCCTCCGCCGCCACTCCCGTCGCCACCCCCACCCCCACCGCCGGTGCCCGCCGCAAGATTGGGGTGGCTGTTGACCTTTCCGAAGAGAGTGCATATGCTGTTCACTGGGCTGTCCAGCACTACATTCGCCCCGGCGATGCTGTCATTCTTCTCCATGTCAGCCCTACTTCCGTCCTCTTCGGCGCCGATTGGGGCTCTATTGACATTACTTTGGATGCCGCCGGTGATAATCCTGACGATGAGGATGCTGCGAATTCTGAAAACGGTAACCGCACCGAGCGCTCCAAACGTAAATTGGAGGATGATTTTGACGCCTTCACTGCCTCGAAAGCTGCCGATATAGCTAAGCCCTTGAAAGATGCTCAGATTCCATACAAGATTCACATAGTCAAGGATCATGATATGAGAGAGAGGCTGTGTTTGGAAGTGGAGAGATTGGGACTGAATGCCCTGATCATGGGCAGTCGGGGTTTTGGTGCAGCAAAGCGCGGAAACGACGGCGGGCTCGGGAGCGTGAGCGATTACTGCGTTCATCATTGTGTGTGTCCTGTGGTGGTGGTGCGTTTTCCGGATGAGAAGGATGTGATCGGGAGCGGTGGTGGTTCTGTGGTGCCGAAGGCGAAGGAAGTCGCCGCCCAGAAAAAGTCTGTAACTGAGGAAGCGGAGTCTAAAGGT

mRNA sequence

ATGGATCCTCTGCGGCAGCTTCGAAATTCCTCCTTAGAACCGGACCCGCAGCTGCCATCCATCAAAATTCACCATCCAGCCTCTCCTCGCCACCCCTCCGCCGCCACTCCCGTCGCCACCCCCACCCCCACCGCCGGTGCCCGCCGCAAGATTGGGGTGGCTGTTGACCTTTCCGAAGAGAGTGCATATGCTGTTCACTGGGCTGTCCAGCACTACATTCGCCCCGGCGATGCTGTCATTCTTCTCCATGTCAGCCCTACTTCCGTCCTCTTCGGCGCCGATTGGGGCTCTATTGACATTACTTTGGATGCCGCCGGTGATAATCCTGACGATGAGGATGCTGCGAATTCTGAAAACGGTAACCGCACCGAGCGCTCCAAACGTAAATTGGAGGATGATTTTGACGCCTTCACTGCCTCGAAAGCTGCCGATATAGCTAAGCCCTTGAAAGATGCTCAGATTCCATACAAGATTCACATAGTCAAGGATCATGATATGAGAGAGAGGCTGTGTTTGGAAGTGGAGAGATTGGGACTGAATGCCCTGATCATGGGCAGTCGGGGTTTTGGTGCAGCAAAGCGCGGAAACGACGGCGGGCTCGGGAGCGTGAGCGATTACTGCGTTCATCATTGTGTGTGTCCTGTGGTGGTGGTGCGTTTTCCGGATGAGAAGGATGTGATCGGGAGCGGTGGTGGTTCTGTGGTGCCGAAGGCGAAGGAAGTCGCCGCCCAGAAAAAGTCTGTAACTGAGGAAGCGGAGTCTAAAGGT

Coding sequence (CDS)

ATGGATCCTCTGCGGCAGCTTCGAAATTCCTCCTTAGAACCGGACCCGCAGCTGCCATCCATCAAAATTCACCATCCAGCCTCTCCTCGCCACCCCTCCGCCGCCACTCCCGTCGCCACCCCCACCCCCACCGCCGGTGCCCGCCGCAAGATTGGGGTGGCTGTTGACCTTTCCGAAGAGAGTGCATATGCTGTTCACTGGGCTGTCCAGCACTACATTCGCCCCGGCGATGCTGTCATTCTTCTCCATGTCAGCCCTACTTCCGTCCTCTTCGGCGCCGATTGGGGCTCTATTGACATTACTTTGGATGCCGCCGGTGATAATCCTGACGATGAGGATGCTGCGAATTCTGAAAACGGTAACCGCACCGAGCGCTCCAAACGTAAATTGGAGGATGATTTTGACGCCTTCACTGCCTCGAAAGCTGCCGATATAGCTAAGCCCTTGAAAGATGCTCAGATTCCATACAAGATTCACATAGTCAAGGATCATGATATGAGAGAGAGGCTGTGTTTGGAAGTGGAGAGATTGGGACTGAATGCCCTGATCATGGGCAGTCGGGGTTTTGGTGCAGCAAAGCGCGGAAACGACGGCGGGCTCGGGAGCGTGAGCGATTACTGCGTTCATCATTGTGTGTGTCCTGTGGTGGTGGTGCGTTTTCCGGATGAGAAGGATGTGATCGGGAGCGGTGGTGGTTCTGTGGTGCCGAAGGCGAAGGAAGTCGCCGCCCAGAAAAAGTCTGTAACTGAGGAAGCGGAGTCTAAAGGT

Protein sequence

MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEESAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENGNRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPKAKEVAAQKKSVTEEAESKG

Homology

[Close]

BLAST of MS021353 vs. NCBI nr
Match: XP_022139521.1 (universal stress protein PHOS32-like [Momordica charantia])

HSP 1 Score: 501.9 bits (1291), Expect = 3.3e-138
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60
           MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE
Sbjct: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60

Query: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120
           SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG
Sbjct: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120

Query: 121 NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLN 180
           NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLN
Sbjct: 121 NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLN 180

Query: 181 ALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPKAKE 240
           ALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPKAKE
Sbjct: 181 ALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPKAKE 240

Query: 241 VAAQKKSVTEEAESK 256
           VAAQKKSVTEEAESK
Sbjct: 241 VAAQKKSVTEEAESK 255

[Close]

BLAST of MS021353 vs. NCBI nr
Match: KAA0036744.1 (universal stress protein PHOS32-like [Cucumis melo var. makuwa] >TYJ95811.1 universal stress protein PHOS32-like [Cucumis melo var. makuwa])

HSP 1 Score: 439.9 bits (1130), Expect = 1.5e-119
Identity = 226/259 (87.26%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60
           MDP   LRN +LEPDPQLP+IKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE
Sbjct: 1   MDPQSYLRNPTLEPDPQLPNIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60

Query: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120
           SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLD  G+NPDD+DA NS+NG
Sbjct: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDTVGNNPDDDDALNSDNG 120

Query: 121 ---NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180
              N TERSKRKLEDDFDAFTASKAAD+AKPLKDAQIPYKIHIVKDHDMRERLCLEVERL
Sbjct: 121 QQQNHTERSKRKLEDDFDAFTASKAADLAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180

Query: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPK 240
           GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV G G  S+  +
Sbjct: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV-GVGASSLAAR 240

Query: 241 AKEVAAQKKSVTEEAESKG 257
             +V A KKSV E  ESKG
Sbjct: 241 KPDVDAPKKSVAEPVESKG 258

[Close]

BLAST of MS021353 vs. NCBI nr
Match: XP_008454617.1 (PREDICTED: uncharacterized protein LOC103494984 [Cucumis melo])

HSP 1 Score: 439.9 bits (1130), Expect = 1.5e-119
Identity = 226/259 (87.26%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60
           MDP   LRN +LEPDPQLP+IKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE
Sbjct: 1   MDPQSYLRNPTLEPDPQLPNIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60

Query: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120
           SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLD  G+NPDD+DA NS+NG
Sbjct: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDTVGNNPDDDDALNSDNG 120

Query: 121 ---NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180
              N TERSKRKLEDDFDAFTASKAAD+AKPLKDAQIPYKIHIVKDHDMRERLCLEVERL
Sbjct: 121 QQQNHTERSKRKLEDDFDAFTASKAADLAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180

Query: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPK 240
           GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV G G  S+  +
Sbjct: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV-GVGASSLAAR 240

Query: 241 AKEVAAQKKSVTEEAESKG 257
             +V A KKSV E  ESKG
Sbjct: 241 KPDVDAPKKSVAEPVESKG 258

[Close]

BLAST of MS021353 vs. NCBI nr
Match: XP_038897925.1 (universal stress protein PHOS32-like [Benincasa hispida] >XP_038897928.1 universal stress protein PHOS32-like [Benincasa hispida])

HSP 1 Score: 439.5 bits (1129), Expect = 2.0e-119
Identity = 227/260 (87.31%), Postives = 239/260 (91.92%), Query Frame = 0


Query: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60
           M+P   LRN +LEPDPQLP+IKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE
Sbjct: 1   MNPQHYLRNPTLEPDPQLPNIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60

Query: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120
           SAYAV WAVQHYIRPGDAVILLHVSPTSVLFGADWG+ID+TLD AGD PDDEDA+NSENG
Sbjct: 61  SAYAVRWAVQHYIRPGDAVILLHVSPTSVLFGADWGAIDMTLDTAGDYPDDEDASNSENG 120

Query: 121 ---NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180
              NRTERSKRKLEDDFDAFTASKAAD+AKPLKDAQIPYKIHIVKDHDMRERLCLEVERL
Sbjct: 121 QQQNRTERSKRKLEDDFDAFTASKAADLAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180

Query: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV-IGSGGGSVVP 240
           GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV IG+G  +V  
Sbjct: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVGIGAGSVAVPV 240

Query: 241 KAKEVAAQKKSVTEEAESKG 257
           + ++V A KKSV E  ESKG
Sbjct: 241 RKEDVDAPKKSVAEAVESKG 260

[Close]

BLAST of MS021353 vs. NCBI nr
Match: XP_011652372.1 (universal stress protein PHOS32 isoform X1 [Cucumis sativus] >KAE8651301.1 hypothetical protein Csa_001286 [Cucumis sativus])

HSP 1 Score: 438.7 bits (1127), Expect = 3.4e-119
Identity = 226/259 (87.26%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60
           MDP   LRN +LEPDPQLP+IKIHHPASPRHPSAATPVATPTPTAGARRK+GVAVDLSEE
Sbjct: 1   MDPHNYLRNPTLEPDPQLPNIKIHHPASPRHPSAATPVATPTPTAGARRKVGVAVDLSEE 60

Query: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120
           SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLD  GDNPDD+ A NSENG
Sbjct: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDTVGDNPDDDGALNSENG 120

Query: 121 ---NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180
              N TERSKRKLEDDFDAFTASKAAD+AKPLKDAQIPYKIHIVKDHDMRERLCLEVERL
Sbjct: 121 QQQNHTERSKRKLEDDFDAFTASKAADLAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180

Query: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPK 240
           GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV G G  S+  +
Sbjct: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV-GVGLSSLAAR 240

Query: 241 AKEVAAQKKSVTEEAESKG 257
             +V A+KK V E AESKG
Sbjct: 241 KPDVDARKKPVAEPAESKG 258

[Close]

BLAST of MS021353 vs. ExPASy Swiss-Prot
Match: Q8L4N1 (Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 9.7e-77
Identity = 150/221 (67.87%), Postives = 173/221 (78.28%), Query Frame = 0


Query: 12  LEPD---PQLPSIKIHHPASPRHP---SAATP-VATPTPTAGARRKIGVAVDLSEESAYA 71
           + PD   P LP+IKIHHP+SPRH    S++TP  ATPTPTAGARRKIGVAVDLSEESA+A
Sbjct: 1   MNPDSDYPHLPNIKIHHPSSPRHSHHHSSSTPSAATPTPTAGARRKIGVAVDLSEESAFA 60

Query: 72  VHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENGNRTE 131
           V WAV HYIRPGDAV++LHVSPTSVLFGADWG + +        P    AA         
Sbjct: 61  VRWAVDHYIRPGDAVVILHVSPTSVLFGADWGPLPL------QTPPPPSAATDPGA---- 120

Query: 132 RSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLNALIM 191
              +  ++DFDAFT+SK AD+AKPLK+A  P+KIHIVKDHDMRERLCLE ERL L+A+IM
Sbjct: 121 -QPKPSQEDFDAFTSSKVADLAKPLKEAGFPHKIHIVKDHDMRERLCLETERLNLSAVIM 180

Query: 192 GSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKD 226
           GSRGFGA KRG+DG LGSVSDYCVHHCVCPVVVVR+PD++D
Sbjct: 181 GSRGFGAEKRGSDGKLGSVSDYCVHHCVCPVVVVRYPDDRD 210

[Close]

BLAST of MS021353 vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.1e-75
Identity = 156/252 (61.90%), Postives = 184/252 (73.02%), Query Frame = 0


Query: 16  PQLPSIKIHHPASPRHP----SAATP--VATPTPTAGARRKIGVAVDLSEESAYAVHWAV 75
           PQLP+IKIHHP SPRH     S++TP   ATPTPTAGARRKIGVAVDLSEES++AV WAV
Sbjct: 9   PQLPNIKIHHPPSPRHSHHHHSSSTPSSAATPTPTAGARRKIGVAVDLSEESSFAVRWAV 68

Query: 76  QHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENGNRTERSKRK 135
            HYIRPGDAV+LLHVSPTSVLFGADWG + +       N   + +               
Sbjct: 69  DHYIRPGDAVVLLHVSPTSVLFGADWGPLPLKTQIEDPNAQPQPS--------------- 128

Query: 136 LEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLNALIMGSRGF 195
            ++DFDAFT++K AD+AKPLK+   PYKIHIVKDHDMRERLCLE+ERLGL+A+IMGSRGF
Sbjct: 129 -QEDFDAFTSTKVADLAKPLKELGFPYKIHIVKDHDMRERLCLEIERLGLSAVIMGSRGF 188

Query: 196 GA-AKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKD------VIGSGG---GSVVPKAK 252
           GA  KRG+DG LGSVSDYCVHHCVCPVVVVR+PD++D       + SGG   G VV  A 
Sbjct: 189 GAEKKRGSDGKLGSVSDYCVHHCVCPVVVVRYPDDRDGPVPIVTVKSGGDDDGDVV--AA 242

[Close]

BLAST of MS021353 vs. ExPASy TrEMBL
Match: A0A6J1CCX8 (universal stress protein PHOS32-like OS=Momordica charantia OX=3673 GN=LOC111010415 PE=4 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 1.6e-138
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60
           MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE
Sbjct: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60

Query: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120
           SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG
Sbjct: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120

Query: 121 NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLN 180
           NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLN
Sbjct: 121 NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLN 180

Query: 181 ALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPKAKE 240
           ALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPKAKE
Sbjct: 181 ALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPKAKE 240

Query: 241 VAAQKKSVTEEAESK 256
           VAAQKKSVTEEAESK
Sbjct: 241 VAAQKKSVTEEAESK 255

[Close]

BLAST of MS021353 vs. ExPASy TrEMBL
Match: A0A5A7SZQ0 (Universal stress protein PHOS32-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G00950 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 7.4e-120
Identity = 226/259 (87.26%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60
           MDP   LRN +LEPDPQLP+IKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE
Sbjct: 1   MDPQSYLRNPTLEPDPQLPNIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60

Query: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120
           SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLD  G+NPDD+DA NS+NG
Sbjct: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDTVGNNPDDDDALNSDNG 120

Query: 121 ---NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180
              N TERSKRKLEDDFDAFTASKAAD+AKPLKDAQIPYKIHIVKDHDMRERLCLEVERL
Sbjct: 121 QQQNHTERSKRKLEDDFDAFTASKAADLAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180

Query: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPK 240
           GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV G G  S+  +
Sbjct: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV-GVGASSLAAR 240

Query: 241 AKEVAAQKKSVTEEAESKG 257
             +V A KKSV E  ESKG
Sbjct: 241 KPDVDAPKKSVAEPVESKG 258

[Close]

BLAST of MS021353 vs. ExPASy TrEMBL
Match: A0A1S3C097 (uncharacterized protein LOC103494984 OS=Cucumis melo OX=3656 GN=LOC103494984 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 7.4e-120
Identity = 226/259 (87.26%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60
           MDP   LRN +LEPDPQLP+IKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE
Sbjct: 1   MDPQSYLRNPTLEPDPQLPNIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60

Query: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120
           SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLD  G+NPDD+DA NS+NG
Sbjct: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDTVGNNPDDDDALNSDNG 120

Query: 121 ---NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180
              N TERSKRKLEDDFDAFTASKAAD+AKPLKDAQIPYKIHIVKDHDMRERLCLEVERL
Sbjct: 121 QQQNHTERSKRKLEDDFDAFTASKAADLAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180

Query: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPK 240
           GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV G G  S+  +
Sbjct: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV-GVGASSLAAR 240

Query: 241 AKEVAAQKKSVTEEAESKG 257
             +V A KKSV E  ESKG
Sbjct: 241 KPDVDAPKKSVAEPVESKG 258

[Close]

BLAST of MS021353 vs. ExPASy TrEMBL
Match: A0A0A0LIJ3 (Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G850650 PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.6e-119
Identity = 226/259 (87.26%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60
           MDP   LRN +LEPDPQLP+IKIHHPASPRHPSAATPVATPTPTAGARRK+GVAVDLSEE
Sbjct: 1   MDPHNYLRNPTLEPDPQLPNIKIHHPASPRHPSAATPVATPTPTAGARRKVGVAVDLSEE 60

Query: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120
           SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLD  GDNPDD+ A NSENG
Sbjct: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDTVGDNPDDDGALNSENG 120

Query: 121 ---NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180
              N TERSKRKLEDDFDAFTASKAAD+AKPLKDAQIPYKIHIVKDHDMRERLCLEVERL
Sbjct: 121 QQQNHTERSKRKLEDDFDAFTASKAADLAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180

Query: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPK 240
           GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV G G  S+  +
Sbjct: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDV-GVGLSSLAAR 240

Query: 241 AKEVAAQKKSVTEEAESKG 257
             +V A+KK V E AESKG
Sbjct: 241 KPDVDARKKPVAEPAESKG 258

[Close]

BLAST of MS021353 vs. ExPASy TrEMBL
Match: A0A6J1J299 (universal stress protein PHOS32-like OS=Cucurbita maxima OX=3661 GN=LOC111480659 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.8e-118
Identity = 225/259 (86.87%), Postives = 231/259 (89.19%), Query Frame = 0


Query: 1   MDPLRQLRNSSLEPDPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEE 60
           MD L  LRN SLEPDPQLP+IKIHHPASPRH S +TPVATPTPTAGARRKIGVAVDLSEE
Sbjct: 1   MDSLNHLRNPSLEPDPQLPNIKIHHPASPRHHSTSTPVATPTPTAGARRKIGVAVDLSEE 60

Query: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENG 120
           SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLD  GDNPDD+DAANSEN 
Sbjct: 61  SAYAVHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDIPGDNPDDQDAANSENS 120

Query: 121 ---NRTERSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERL 180
              NRTERSKRK+EDDFDAFTASKAAD+AKPLKD QIPYKIHIVKDHDMRERLCLEVERL
Sbjct: 121 HRQNRTERSKRKMEDDFDAFTASKAADLAKPLKDVQIPYKIHIVKDHDMRERLCLEVERL 180

Query: 181 GLNALIMGSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKDVIGSGGGSVVPK 240
           GLNALIMGSRGFGAAKRGND GLGSVSDYCVHHC CPVVVVRFPDEKDV G G GSV   
Sbjct: 181 GLNALIMGSRGFGAAKRGNDSGLGSVSDYCVHHCACPVVVVRFPDEKDV-GIGSGSVAVA 240

Query: 241 AKEVAAQKKSVTEEAESKG 257
            K+  AQKKS  EE E KG
Sbjct: 241 KKDDDAQKKSAAEEVEPKG 258

[Close]

BLAST of MS021353 vs. TAIR 10
Match: AT4G27320.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 6.9e-78
Identity = 150/221 (67.87%), Postives = 173/221 (78.28%), Query Frame = 0


Query: 12  LEPD---PQLPSIKIHHPASPRHP---SAATP-VATPTPTAGARRKIGVAVDLSEESAYA 71
           + PD   P LP+IKIHHP+SPRH    S++TP  ATPTPTAGARRKIGVAVDLSEESA+A
Sbjct: 1   MNPDSDYPHLPNIKIHHPSSPRHSHHHSSSTPSAATPTPTAGARRKIGVAVDLSEESAFA 60

Query: 72  VHWAVQHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENGNRTE 131
           V WAV HYIRPGDAV++LHVSPTSVLFGADWG + +        P    AA         
Sbjct: 61  VRWAVDHYIRPGDAVVILHVSPTSVLFGADWGPLPL------QTPPPPSAATDPGA---- 120

Query: 132 RSKRKLEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLNALIM 191
              +  ++DFDAFT+SK AD+AKPLK+A  P+KIHIVKDHDMRERLCLE ERL L+A+IM
Sbjct: 121 -QPKPSQEDFDAFTSSKVADLAKPLKEAGFPHKIHIVKDHDMRERLCLETERLNLSAVIM 180

Query: 192 GSRGFGAAKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKD 226
           GSRGFGA KRG+DG LGSVSDYCVHHCVCPVVVVR+PD++D
Sbjct: 181 GSRGFGAEKRGSDGKLGSVSDYCVHHCVCPVVVVRYPDDRD 210

[Close]

BLAST of MS021353 vs. TAIR 10
Match: AT5G54430.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 284.6 bits (727), Expect = 7.6e-77
Identity = 156/252 (61.90%), Postives = 184/252 (73.02%), Query Frame = 0


Query: 16  PQLPSIKIHHPASPRHP----SAATP--VATPTPTAGARRKIGVAVDLSEESAYAVHWAV 75
           PQLP+IKIHHP SPRH     S++TP   ATPTPTAGARRKIGVAVDLSEES++AV WAV
Sbjct: 9   PQLPNIKIHHPPSPRHSHHHHSSSTPSSAATPTPTAGARRKIGVAVDLSEESSFAVRWAV 68

Query: 76  QHYIRPGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENGNRTERSKRK 135
            HYIRPGDAV+LLHVSPTSVLFGADWG + +       N   + +               
Sbjct: 69  DHYIRPGDAVVLLHVSPTSVLFGADWGPLPLKTQIEDPNAQPQPS--------------- 128

Query: 136 LEDDFDAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLNALIMGSRGF 195
            ++DFDAFT++K AD+AKPLK+   PYKIHIVKDHDMRERLCLE+ERLGL+A+IMGSRGF
Sbjct: 129 -QEDFDAFTSTKVADLAKPLKELGFPYKIHIVKDHDMRERLCLEIERLGLSAVIMGSRGF 188

Query: 196 GA-AKRGNDGGLGSVSDYCVHHCVCPVVVVRFPDEKD------VIGSGG---GSVVPKAK 252
           GA  KRG+DG LGSVSDYCVHHCVCPVVVVR+PD++D       + SGG   G VV  A 
Sbjct: 189 GAEKKRGSDGKLGSVSDYCVHHCVCPVVVVRYPDDRDGPVPIVTVKSGGDDDGDVV--AA 242

[Close]

BLAST of MS021353 vs. TAIR 10
Match: AT1G11360.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 6.5e-68
Identity = 140/246 (56.91%), Postives = 173/246 (70.33%), Query Frame = 0


Query: 15  DPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEESAYAVHWAVQHYIR 74
           D + P++    P+SPR P +       TPTAGA+RKIG+AVDLS+ESAYAV WAVQ+Y+R
Sbjct: 11  DRKSPTVVTVQPSSPRFPIS-------TPTAGAQRKIGIAVDLSDESAYAVQWAVQNYLR 70

Query: 75  PGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENGNRTERSKRKLEDDF 134
            GDAV+LLHV PTSVL+GADWG++D++     +N               E S+RKLEDDF
Sbjct: 71  SGDAVVLLHVQPTSVLYGADWGAMDLSPQWDPNN---------------EESQRKLEDDF 130

Query: 135 DAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLNALIMGSRGFGAAKR 194
           D  T  KA+D+A+PL +A IP+KIHIVKDHDM+ERLCLEVERLGL+ LIMGSRGFGA KR
Sbjct: 131 DIVTNKKASDVAQPLVEADIPFKIHIVKDHDMKERLCLEVERLGLSTLIMGSRGFGATKR 190

Query: 195 GNDGGLGSVSDYCVHHCVCPVVVVRFPDEKD----VIGSGGGSVVPKAKEVAAQKKSVTE 254
            + G LGSVSDY VHHC CPVVVVRFPD+KD      G  GG  +  + ++     +V E
Sbjct: 191 SSKGRLGSVSDYSVHHCACPVVVVRFPDDKDGEDEKSGDSGGENLMDSDKL----HTVPE 230

Query: 255 EAESKG 257
            AE +G
Sbjct: 251 VAEEEG 230

[Close]

BLAST of MS021353 vs. TAIR 10
Match: AT1G11360.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 6.5e-68
Identity = 140/246 (56.91%), Postives = 173/246 (70.33%), Query Frame = 0


Query: 15  DPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEESAYAVHWAVQHYIR 74
           D + P++    P+SPR P +       TPTAGA+RKIG+AVDLS+ESAYAV WAVQ+Y+R
Sbjct: 11  DRKSPTVVTVQPSSPRFPIS-------TPTAGAQRKIGIAVDLSDESAYAVQWAVQNYLR 70

Query: 75  PGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENGNRTERSKRKLEDDF 134
            GDAV+LLHV PTSVL+GADWG++D++     +N               E S+RKLEDDF
Sbjct: 71  SGDAVVLLHVQPTSVLYGADWGAMDLSPQWDPNN---------------EESQRKLEDDF 130

Query: 135 DAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLNALIMGSRGFGAAKR 194
           D  T  KA+D+A+PL +A IP+KIHIVKDHDM+ERLCLEVERLGL+ LIMGSRGFGA KR
Sbjct: 131 DIVTNKKASDVAQPLVEADIPFKIHIVKDHDMKERLCLEVERLGLSTLIMGSRGFGATKR 190

Query: 195 GNDGGLGSVSDYCVHHCVCPVVVVRFPDEKD----VIGSGGGSVVPKAKEVAAQKKSVTE 254
            + G LGSVSDY VHHC CPVVVVRFPD+KD      G  GG  +  + ++     +V E
Sbjct: 191 SSKGRLGSVSDYSVHHCACPVVVVRFPDDKDGEDEKSGDSGGENLMDSDKL----HTVPE 230

Query: 255 EAESKG 257
            AE +G
Sbjct: 251 VAEEEG 230

[Close]

BLAST of MS021353 vs. TAIR 10
Match: AT1G11360.3 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 6.5e-68
Identity = 140/246 (56.91%), Postives = 173/246 (70.33%), Query Frame = 0


Query: 15  DPQLPSIKIHHPASPRHPSAATPVATPTPTAGARRKIGVAVDLSEESAYAVHWAVQHYIR 74
           D + P++    P+SPR P +       TPTAGA+RKIG+AVDLS+ESAYAV WAVQ+Y+R
Sbjct: 11  DRKSPTVVTVQPSSPRFPIS-------TPTAGAQRKIGIAVDLSDESAYAVQWAVQNYLR 70

Query: 75  PGDAVILLHVSPTSVLFGADWGSIDITLDAAGDNPDDEDAANSENGNRTERSKRKLEDDF 134
            GDAV+LLHV PTSVL+GADWG++D++     +N               E S+RKLEDDF
Sbjct: 71  SGDAVVLLHVQPTSVLYGADWGAMDLSPQWDPNN---------------EESQRKLEDDF 130

Query: 135 DAFTASKAADIAKPLKDAQIPYKIHIVKDHDMRERLCLEVERLGLNALIMGSRGFGAAKR 194
           D  T  KA+D+A+PL +A IP+KIHIVKDHDM+ERLCLEVERLGL+ LIMGSRGFGA KR
Sbjct: 131 DIVTNKKASDVAQPLVEADIPFKIHIVKDHDMKERLCLEVERLGLSTLIMGSRGFGATKR 190

Query: 195 GNDGGLGSVSDYCVHHCVCPVVVVRFPDEKD----VIGSGGGSVVPKAKEVAAQKKSVTE 254
            + G LGSVSDY VHHC CPVVVVRFPD+KD      G  GG  +  + ++     +V E
Sbjct: 191 SSKGRLGSVSDYSVHHCACPVVVVRFPDDKDGEDEKSGDSGGENLMDSDKL----HTVPE 230

Query: 255 EAESKG 257
            AE +G
Sbjct: 251 VAEEEG 230

The following BLAST results are available for this feature:

BLAST of MS021353 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139521.1	3.3e-138	100.00	universal stress protein PHOS32-like [Momordica charantia]	[more]
KAA0036744.1	1.5e-119	87.26	universal stress protein PHOS32-like [Cucumis melo var. makuwa] >TYJ95811.1 univ...	[more]
XP_008454617.1	1.5e-119	87.26	PREDICTED: uncharacterized protein LOC103494984 [Cucumis melo]	[more]
XP_038897925.1	2.0e-119	87.31	universal stress protein PHOS32-like [Benincasa hispida] >XP_038897928.1 univers...	[more]
XP_011652372.1	3.4e-119	87.26	universal stress protein PHOS32 isoform X1 [Cucumis sativus] >KAE8651301.1 hypot...	[more]

BLAST of MS021353 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L4N1	9.7e-77	67.87	Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 S...	[more]
Q8VYN9	1.1e-75	61.90	Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S...	[more]

BLAST of MS021353 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CCX8	1.6e-138	100.00	universal stress protein PHOS32-like OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A5A7SZQ0	7.4e-120	87.26	Universal stress protein PHOS32-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3C097	7.4e-120	87.26	uncharacterized protein LOC103494984 OS=Cucumis melo OX=3656 GN=LOC103494984 PE=...	[more]
A0A0A0LIJ3	1.6e-119	87.26	Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G850650 PE=4 SV...	[more]
A0A6J1J299	1.8e-118	86.87	universal stress protein PHOS32-like OS=Cucurbita maxima OX=3661 GN=LOC111480659...	[more]

BLAST of MS021353 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27320.1	6.9e-78	67.87	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT5G54430.1	7.6e-77	61.90	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT1G11360.1	6.5e-68	56.91	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT1G11360.2	6.5e-68	56.91	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT1G11360.3	6.5e-68	56.91	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006015	Universal stress protein A family	PRINTS	PR01438	UNVRSLSTRESS	coord: 48..66 score: 32.21 coord: 197..219 score: 37.74
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 42..227 e-value: 9.1E-33 score: 116.0
IPR006016	UspA	PFAM	PF00582	Usp	coord: 48..219 e-value: 9.4E-28 score: 97.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 106..128
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..45
None	No IPR available	PANTHER	PTHR31966:SF18	UNIVERSAL STRESS PROTEIN PHOS32	coord: 6..243
None	No IPR available	CDD	cd00293	USP_Like	coord: 50..206 e-value: 1.11116E-14 score: 67.0098
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 49..221
IPR044162	Universal stress protein PHOS32/34	PANTHER	PTHR31966	OS01G0783500 PROTEIN	coord: 6..243

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021353.1	MS021353.1	mRNA