MS021351 (gene) Bitter gourd (TR) v1

Overview


Name	MS021351
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protein SPOROCYTELESS
Location	scaffold358: 1293094 .. 1294285 (+)
RNA-Seq Expression	MS021351
Synteny	MS021351

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACTCCAATGCTTCTCATGGCCGCCCAAAAATCCAACGCCAACGCATCACAAGAAACCAAGCCAGAGCCCACCAAAACCAGACCCTCAAGATCGTCAAAGTCCGCCCCCAAAGCCCCCGCCCACAAGAAGCCGCCGCAGCGCGGCCTCGGCGTCGCCCAGCTCGAGCGCCTCCGCCTCCAGGAACGCTGGAAGAAAATGACCGAAATGCCCCCGCCGCCCGTCCACGCCCACCACTTCGCCAACATTCCCGGCCTCCACTTCTCCGCCGTTGATGACTGTACTGGCGAAGGCGGTGGTCTCGTCTTTCAGGGGATGGGGAATGTTGGAGGGTTTGTCGCCGGCGCCGGAGGGTTCACGGTGGTGGAGCCGTACACGCACGGCGGCGGAGCCCTGGATCCGAGGGTTCTGATCGGAAGTTACGGCGAGGAGGATTTGAGAGAGCTCTCTTCAATCCCAAAAATGCCGATGCCGTGCGTTTCCGATCGCTGTGATATTTGCTTCAAGGTAATATTTATTATTCTTCGTATCAACTTGTTTGGATTCCGAGAAAATTTCCGTGACGAGAAAACGGTTATACATATTCATCTCCGTTTTCCGACGCCCAAATCGATTAAATTAATTACTCACGCCATTTTTCTGAGTTCACGTTTTAATCAAACAGAAGAAACGCGTCAACATTTCCACAGAACTGCCGGCGGCGGCGGCCATTATCAACACTGACAGTTACGATTTTCTCGGACTGAGCACAAACTCCGCCGCGAGAGGCGGCGGCGGAACCAACCAGGTGATCAAATCAAAACGAAAACCACACCAAAAACAAGAAAACAAAAAAACCCACTACGGAAAATTTGAAGAAGAACAAAATTAATTAATTTGTTTGGTGCAGGGCGGAGCAGAGATCAAGCAAATACACAGGAAATTAGTGGGGAACGGCGGAGGAGGAGGAGAAAATATATTAATGGAATACGAGTTTTTTCCTGGGAAAAATGGCAGAGGCACAGAGTTCAAGGAACTGGAAATGCCAAAGGAAGAAGAAGAAGAAGAATTTTCGTTTGCAGAAGAAGCAGTGGATCATGGAGAAGCTTCGTCTTGTATTACTACAACCTACAACACTGCCATTGTTAATAATAATGGCAGCAGTAGCAGTGGTTCCAGTGCAGTTGATTTGTCTCTCAAACTTTCATTT

mRNA sequence

ATGGCTACTCCAATGCTTCTCATGGCCGCCCAAAAATCCAACGCCAACGCATCACAAGAAACCAAGCCAGAGCCCACCAAAACCAGACCCTCAAGATCGTCAAAGTCCGCCCCCAAAGCCCCCGCCCACAAGAAGCCGCCGCAGCGCGGCCTCGGCGTCGCCCAGCTCGAGCGCCTCCGCCTCCAGGAACGCTGGAAGAAAATGACCGAAATGCCCCCGCCGCCCGTCCACGCCCACCACTTCGCCAACATTCCCGGCCTCCACTTCTCCGCCGTTGATGACTGTACTGGCGAAGGCGGTGGTCTCGTCTTTCAGGGGATGGGGAATGTTGGAGGGTTTGTCGCCGGCGCCGGAGGGTTCACGGTGGTGGAGCCGTACACGCACGGCGGCGGAGCCCTGGATCCGAGGGTTCTGATCGGAAGTTACGGCGAGGAGGATTTGAGAGAGCTCTCTTCAATCCCAAAAATGCCGATGCCGTGCGTTTCCGATCGCTGTGATATTTGCTTCAAGGTAATATTTATTATTCTTCGTATCAACTTGTTTGGATTCCGAGAAAATTTCCGTGACGAGAAAACGGTTATACATATTCATCTCCGTTTTCCGACGCCCAAATCGATTAAATTAATTACTCACGCCATTTTTCTGAGTTCACGTTTTAATCAAACAGAAGAAACGCTGGGGAACGGCGGAGGAGGAGGAGAAAATATATTAATGGAATACGAGTTTTTTCCTGGGAAAAATGGCAGAGGCACAGAGTTCAAGGAACTGGAAATGCCAAAGGAAGAAGAAGAAGAAGAATTTTCGTTTGCAGAAGAAGCAGTGGATCATGGAGAAGCTTCGTCTTGTATTACTACAACCTACAACACTGCCATTGTTAATAATAATGGCAGCAGTAGCAGTGGTTCCAGTGCAGTTGATTTGTCTCTCAAACTTTCATTT

Coding sequence (CDS)

ATGGCTACTCCAATGCTTCTCATGGCCGCCCAAAAATCCAACGCCAACGCATCACAAGAAACCAAGCCAGAGCCCACCAAAACCAGACCCTCAAGATCGTCAAAGTCCGCCCCCAAAGCCCCCGCCCACAAGAAGCCGCCGCAGCGCGGCCTCGGCGTCGCCCAGCTCGAGCGCCTCCGCCTCCAGGAACGCTGGAAGAAAATGACCGAAATGCCCCCGCCGCCCGTCCACGCCCACCACTTCGCCAACATTCCCGGCCTCCACTTCTCCGCCGTTGATGACTGTACTGGCGAAGGCGGTGGTCTCGTCTTTCAGGGGATGGGGAATGTTGGAGGGTTTGTCGCCGGCGCCGGAGGGTTCACGGTGGTGGAGCCGTACACGCACGGCGGCGGAGCCCTGGATCCGAGGGTTCTGATCGGAAGTTACGGCGAGGAGGATTTGAGAGAGCTCTCTTCAATCCCAAAAATGCCGATGCCGTGCGTTTCCGATCGCTGTGATATTTGCTTCAAGGTAATATTTATTATTCTTCGTATCAACTTGTTTGGATTCCGAGAAAATTTCCGTGACGAGAAAACGGTTATACATATTCATCTCCGTTTTCCGACGCCCAAATCGATTAAATTAATTACTCACGCCATTTTTCTGAGTTCACGTTTTAATCAAACAGAAGAAACGCTGGGGAACGGCGGAGGAGGAGGAGAAAATATATTAATGGAATACGAGTTTTTTCCTGGGAAAAATGGCAGAGGCACAGAGTTCAAGGAACTGGAAATGCCAAAGGAAGAAGAAGAAGAAGAATTTTCGTTTGCAGAAGAAGCAGTGGATCATGGAGAAGCTTCGTCTTGTATTACTACAACCTACAACACTGCCATTGTTAATAATAATGGCAGCAGTAGCAGTGGTTCCAGTGCAGTTGATTTGTCTCTCAAACTTTCATTT

Protein sequence

MATPMLLMAAQKSNANASQETKPEPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERLRLQERWKKMTEMPPPPVHAHHFANIPGLHFSAVDDCTGEGGGLVFQGMGNVGGFVAGAGGFTVVEPYTHGGGALDPRVLIGSYGEEDLRELSSIPKMPMPCVSDRCDICFKVIFIILRINLFGFRENFRDEKTVIHIHLRFPTPKSIKLITHAIFLSSRFNQTEETLGNGGGGGENILMEYEFFPGKNGRGTEFKELEMPKEEEEEEFSFAEEAVDHGEASSCITTTYNTAIVNNNGSSSSGSSAVDLSLKLSF

Homology

[Close]

BLAST of MS021351 vs. NCBI nr
Match: XP_022975733.1 (protein SPOROCYTELESS [Cucurbita maxima])

HSP 1 Score: 197.6 bits (501), Expect = 1.6e-46
Identity = 164/350 (46.86%), Postives = 201/350 (57.43%), Query Frame = 0


Query: 1   MATPMLLMAAQKSNANASQETKP-EPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERL 60
           MATPM+++          +ETKP EP KTR +R  K+A K P  KKPPQRGLGVAQLERL
Sbjct: 1   MATPMVVI----------EETKPGEPLKTRAAR--KTAAKNPHQKKPPQRGLGVAQLERL 60

Query: 61  RLQERWKKMTEMPPPPVHAHHFA---NIPGLHFSAVDDCTGEGGGLVFQGMGNVGGFVAG 120
           RLQERWKKMT++ PP      F     + G   + V +  G G   V   +GN  GF +G
Sbjct: 61  RLQERWKKMTQISPPHPFLLDFPLQFPVAGASSAPVGNDYGTG---VLGFIGNC-GFGSG 120

Query: 121 AGGFTVVEPYTHGGGAL-DPRVLIGSYGEEDLRELSSIPKM-----PMPCVSDRCDICFK 180
            GG   +EP+ HGGGA+ DPR+LIG+   E  RELSSIP +     P PCVSDRCDICFK
Sbjct: 121 GGGLMTMEPFPHGGGAMVDPRLLIGN-SVEASRELSSIPNLPPPPPPPPCVSDRCDICFK 180

Query: 181 VIFIILRINLFGFRENFRDEKTVIHIHLRFPTPKS---IKLITHA----IFLSSRFNQT- 240
                 R+N      N   EK +  I    P P S   + L T++       S  FNQ  
Sbjct: 181 K----KRVNF----SNTMKEKNI--IGTAEPPPVSFDFLGLSTNSAATDFDFSLNFNQDG 240

Query: 241 -------EETLGNGGGGGE-NILMEYEFFPGKNGRGTEFKELEMP-----------KEEE 300
                   +  G GGGGGE + LMEYEFFP KNGRGTEF+EL+ P           +EE+
Sbjct: 241 AGVKQIHRKVAGKGGGGGEGSTLMEYEFFPRKNGRGTEFEELKRPNEELGLFAEEEEEEQ 300

Query: 301 EEEFSFAEEAVDHGEASSCITTTYNTAIVNNNGSSSSGSSAVDLSLKLSF 314
           EEE   A +AVDHGE  SCITT+ +  I   NG + + S+ +DLSLKLSF
Sbjct: 301 EEEEEEAVQAVDHGE-GSCITTSCSDII---NGGTRN-STVLDLSLKLSF 318

[Close]

BLAST of MS021351 vs. NCBI nr
Match: XP_022936226.1 (protein virilizer homolog [Cucurbita moschata])

HSP 1 Score: 190.7 bits (483), Expect = 2.0e-44
Identity = 167/362 (46.13%), Postives = 199/362 (54.97%), Query Frame = 0


Query: 1   MATPMLLMAAQKSNANASQETKP-EPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERL 60
           MA+PM+++          +ETKP EP KTR +R  K+A K P HKKPPQRGLGVAQLERL
Sbjct: 1   MASPMVVI----------EETKPGEPLKTRAAR--KTAAKNPHHKKPPQRGLGVAQLERL 60

Query: 61  RLQERWKKMTEMPPP---PVHAHHFANIPGLHFSAV-----DDCTGEGGGLVFQGMGNVG 120
           RLQERWKKMTE+ PP     H+    + P L FSA      D  TG  G   F G G  G
Sbjct: 61  RLQERWKKMTEISPPHPFQSHSPFLLDFP-LQFSAAAAGGNDYATGVLG---FIGNGGFG 120

Query: 121 GFVAGAGGFTVVEPYTHGGGAL-DPRVLIGSYGEEDLRELSSIPKM---------PMPCV 180
           G   G GG   +EP+ HGGGA+ DPR+LIG+   E  RELSSIP +         P PCV
Sbjct: 121 G-GGGGGGLMTMEPFPHGGGAMVDPRLLIGN-SVEASRELSSIPNLPPPPPPPPPPPPCV 180

Query: 181 SDRCDICFKVIFIILRINLFGFRENFRDEKTVIHIHLRFPTP-----KSIKLITHA---- 240
           SDRCDICFK      R+N      N   EK +I      P P       + L T++    
Sbjct: 181 SDRCDICFKK----KRVNF----SNTMKEKNIIGTAEPPPPPAVASFDFLGLNTNSAATD 240

Query: 241 IFLSSRFNQT--------EETLGNGGGGG--ENILMEYEFFPGKNGRGTEFKE------- 300
              S  FNQ          +  G GGGGG   + LMEYEFFP KNGRGTE +E       
Sbjct: 241 FDFSLNFNQEGAGVKQIHRKVAGKGGGGGGEGSTLMEYEFFPRKNGRGTELEERKRANEE 300

Query: 301 ----LEMPKEEEEEEFSFAEEAVDHGEASSCITTTYNTAIVNNNGSSSSGSSAVDLSLKL 314
                E  +EEEEEE   A +AVDHGE  SCITT+ +  I   NG + + S+ +DLSLKL
Sbjct: 301 LGLFAEEEEEEEEEEEEEAVQAVDHGE-GSCITTSCSDII---NGGTRN-STVLDLSLKL 331

[Close]

BLAST of MS021351 vs. NCBI nr
Match: KAG7024875.1 (hypothetical protein SDJN02_13694, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 187.6 bits (475), Expect = 1.7e-43
Identity = 166/362 (45.86%), Postives = 199/362 (54.97%), Query Frame = 0


Query: 1   MATPMLLMAAQKSNANASQETKP-EPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERL 60
           MA+PM+++          +ETKP EP KTR +R  K+A K P HKKPPQRGLGVAQLERL
Sbjct: 1   MASPMVVI----------EETKPGEPHKTRAAR--KTAAKNPHHKKPPQRGLGVAQLERL 60

Query: 61  RLQERWKKMTEMPPP---PVHAHHFANIPGLHFSAV-----DDCTGEGGGLVFQGMGNVG 120
           RLQERWKKMTE+ PP     H+    + P L F A      D  TG  G   F G G  G
Sbjct: 61  RLQERWKKMTEISPPHPFQSHSPFLLDFP-LQFPAAAAGGNDYATGVLG---FIGNGGFG 120

Query: 121 GFVAGAGGFTVVEPYTHGGGAL-DPRVLIGSYGEEDLRELSSIPKM---------PMPCV 180
           G   G GG   +EP+ HGGGA+ DPR+LIG+   E  RELSSIP +         P PCV
Sbjct: 121 G-GGGGGGLMTMEPFPHGGGAMVDPRLLIGN-SVEASRELSSIPNLPPPPPPPPPPPPCV 180

Query: 181 SDRCDICFKVIFIILRINLFGFRENFRDEKTVIHIHLRFPTPKS-----IKLITHA---- 240
           SDRCDICFK      R+N      N   EK +I      P P +     + L T++    
Sbjct: 181 SDRCDICFKK----KRVNF----SNTMKEKNIIGT-AEPPPPAAASFDFLGLNTNSAATD 240

Query: 241 IFLSSRFNQT--------EETLGNGGGGG--ENILMEYEFFPGKNGRGTEFKE------- 300
              S  FNQ          +  G GGGGG   + LMEYEFFP KNGRGTE +E       
Sbjct: 241 FDFSLNFNQDGAGVKQIHRKVAGKGGGGGGEGSTLMEYEFFPRKNGRGTELEERKRANEE 300

Query: 301 ----LEMPKEEEEEEFSFAEEAVDHGEASSCITTTYNTAIVNNNGSSSSGSSAVDLSLKL 314
                E  +EEEEEE   A +AVDHGE  SCITT+ +  I   NG + + S+ +DLSLKL
Sbjct: 301 LGLFAEEEEEEEEEEEEEAVQAVDHGE-GSCITTSCSDII---NGGTRN-STVLDLSLKL 330

[Close]

BLAST of MS021351 vs. NCBI nr
Match: KAG6591999.1 (hypothetical protein SDJN03_14345, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 186.0 bits (471), Expect = 4.8e-43
Identity = 165/365 (45.21%), Postives = 198/365 (54.25%), Query Frame = 0


Query: 1   MATPMLLMAAQKSNANASQETKP-EPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERL 60
           MA+PM+++          +ETKP EP KTR +R  K+A K P HKKPPQRGLGVAQLERL
Sbjct: 1   MASPMVVI----------EETKPGEPLKTRAAR--KTAAKNPHHKKPPQRGLGVAQLERL 60

Query: 61  RLQERWKKMTEMPPP---PVHAHHFANIPGLHFSAV-----DDCTGEGGGLVFQGMGNVG 120
           RLQERWKKMTE+ PP     H+    + P L F A      D  TG  G   F G G  G
Sbjct: 61  RLQERWKKMTEISPPHPFQSHSPFLLDFP-LQFPAAAAGGNDYATGVLG---FIGNGGFG 120

Query: 121 GFVAGAGGFTVVEPYTHGGGAL-DPRVLIGSYGEEDLRELSSIPKM---------PMPCV 180
           G   G GG   +EP+ HGGGA+ D R+LIG+   E  RELSSIP +         P PCV
Sbjct: 121 G--GGCGGLMTMEPFPHGGGAMVDHRLLIGN-SVEASRELSSIPNLPPPPPPPPPPPPCV 180

Query: 181 SDRCDICFKVIFIILRINLFGFRENFRDEKTVIHIHLRFPTPKS-----IKLITHA---- 240
           SDRCDICFK      R+N      N   EK +I      P P +     + L T++    
Sbjct: 181 SDRCDICFK-----KRVNF----SNTMKEKNIIGTAEPPPPPAAASFDFLGLNTNSAATD 240

Query: 241 IFLSSRFNQT--------EETLGNGGGGG--ENILMEYEFFPGKNGRGTEFKEL------ 300
              S  FNQ          +  G GGGGG   + LMEYEFFP KNGRGTE +E       
Sbjct: 241 FDFSLNFNQDGAGVKQIHRKVAGKGGGGGGEGSTLMEYEFFPRKNGRGTELEERKRANEE 300

Query: 301 --------EMPKEEEEEEFSFAEEAVDHGEASSCITTTYNTAIVNNNGSSSSGSSAVDLS 314
                   E  +EEEEEE   A +AVDHGE  SCITT+ +  I   NG + + S+ +DLS
Sbjct: 301 LGLFAEEEEEEEEEEEEEEEEAVQAVDHGE-GSCITTSCSDII---NGGTRN-STVLDLS 332

[Close]

BLAST of MS021351 vs. NCBI nr
Match: XP_038900102.1 (protein SPOROCYTELESS-like [Benincasa hispida])

HSP 1 Score: 176.0 bits (445), Expect = 5.0e-40
Identity = 159/366 (43.44%), Postives = 186/366 (50.82%), Query Frame = 0


Query: 19  QETKP-EPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERLRLQERWKKMTEMPPPPVH 78
           +ETKP EP KTRP R  K+  + P  KKPPQRGLGVAQLERLRLQ++W K+TEM PP   
Sbjct: 10  EETKPHEPPKTRPGR--KTGARNPHQKKPPQRGLGVAQLERLRLQDKWNKITEMSPP--- 69

Query: 79  AHHF--------------ANIPGLHFSA------VDDCTGEGG-------GLVFQGMGNV 138
            HHF               N P L F A        D  G GG       GLV Q +GN 
Sbjct: 70  -HHFIQSHSPTFLLDNTLTNFP-LQFPAAAPVMVATDSGGGGGVLGFDHQGLVVQRIGNH 129

Query: 139 GGFVAGAGGFTVVEPYTHGGGALDPRVLIGSYGEEDLRELSSIPKMPMP-----CVSDRC 198
           GGF+AG       EPY+HGGG     VLIG+   E  RELSSIPK+P P     C SD C
Sbjct: 130 GGFLAGG------EPYSHGGG-----VLIGNSSVEASRELSSIPKLPPPSLPPSCDSDHC 189

Query: 199 DICFKVIFIILRINLFGFRENFRDEKTVIHIHLRFP-------------TPKSIKLITHA 258
           DICFK      R+N      N   EK +       P             T  S   + ++
Sbjct: 190 DICFKK----KRVNF----SNLMKEKNINIAAAETPPLAAAGFDFLGLSTTNSTAELNNS 249

Query: 259 IFLSS------------RFNQ----TEETLGNGGGG----GENILMEYEFFPGKNGRGTE 314
             ++              FNQ         G+GGGG    G + LMEYEFFP KN RGTE
Sbjct: 250 TVVNHHANPDFGFSFNFNFNQGRSGGNSGSGSGGGGGNGEGSSRLMEYEFFPRKNCRGTE 309

[Close]

BLAST of MS021351 vs. ExPASy TrEMBL
Match: A0A6J1IK53 (protein SPOROCYTELESS OS=Cucurbita maxima OX=3661 GN=LOC111475949 PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 7.8e-47
Identity = 164/350 (46.86%), Postives = 201/350 (57.43%), Query Frame = 0


Query: 1   MATPMLLMAAQKSNANASQETKP-EPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERL 60
           MATPM+++          +ETKP EP KTR +R  K+A K P  KKPPQRGLGVAQLERL
Sbjct: 1   MATPMVVI----------EETKPGEPLKTRAAR--KTAAKNPHQKKPPQRGLGVAQLERL 60

Query: 61  RLQERWKKMTEMPPPPVHAHHFA---NIPGLHFSAVDDCTGEGGGLVFQGMGNVGGFVAG 120
           RLQERWKKMT++ PP      F     + G   + V +  G G   V   +GN  GF +G
Sbjct: 61  RLQERWKKMTQISPPHPFLLDFPLQFPVAGASSAPVGNDYGTG---VLGFIGNC-GFGSG 120

Query: 121 AGGFTVVEPYTHGGGAL-DPRVLIGSYGEEDLRELSSIPKM-----PMPCVSDRCDICFK 180
            GG   +EP+ HGGGA+ DPR+LIG+   E  RELSSIP +     P PCVSDRCDICFK
Sbjct: 121 GGGLMTMEPFPHGGGAMVDPRLLIGN-SVEASRELSSIPNLPPPPPPPPCVSDRCDICFK 180

Query: 181 VIFIILRINLFGFRENFRDEKTVIHIHLRFPTPKS---IKLITHA----IFLSSRFNQT- 240
                 R+N      N   EK +  I    P P S   + L T++       S  FNQ  
Sbjct: 181 K----KRVNF----SNTMKEKNI--IGTAEPPPVSFDFLGLSTNSAATDFDFSLNFNQDG 240

Query: 241 -------EETLGNGGGGGE-NILMEYEFFPGKNGRGTEFKELEMP-----------KEEE 300
                   +  G GGGGGE + LMEYEFFP KNGRGTEF+EL+ P           +EE+
Sbjct: 241 AGVKQIHRKVAGKGGGGGEGSTLMEYEFFPRKNGRGTEFEELKRPNEELGLFAEEEEEEQ 300

Query: 301 EEEFSFAEEAVDHGEASSCITTTYNTAIVNNNGSSSSGSSAVDLSLKLSF 314
           EEE   A +AVDHGE  SCITT+ +  I   NG + + S+ +DLSLKLSF
Sbjct: 301 EEEEEEAVQAVDHGE-GSCITTSCSDII---NGGTRN-STVLDLSLKLSF 318

[Close]

BLAST of MS021351 vs. ExPASy TrEMBL
Match: A0A6J1F6X7 (protein virilizer homolog OS=Cucurbita moschata OX=3662 GN=LOC111442900 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 9.5e-45
Identity = 167/362 (46.13%), Postives = 199/362 (54.97%), Query Frame = 0


Query: 1   MATPMLLMAAQKSNANASQETKP-EPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERL 60
           MA+PM+++          +ETKP EP KTR +R  K+A K P HKKPPQRGLGVAQLERL
Sbjct: 1   MASPMVVI----------EETKPGEPLKTRAAR--KTAAKNPHHKKPPQRGLGVAQLERL 60

Query: 61  RLQERWKKMTEMPPP---PVHAHHFANIPGLHFSAV-----DDCTGEGGGLVFQGMGNVG 120
           RLQERWKKMTE+ PP     H+    + P L FSA      D  TG  G   F G G  G
Sbjct: 61  RLQERWKKMTEISPPHPFQSHSPFLLDFP-LQFSAAAAGGNDYATGVLG---FIGNGGFG 120

Query: 121 GFVAGAGGFTVVEPYTHGGGAL-DPRVLIGSYGEEDLRELSSIPKM---------PMPCV 180
           G   G GG   +EP+ HGGGA+ DPR+LIG+   E  RELSSIP +         P PCV
Sbjct: 121 G-GGGGGGLMTMEPFPHGGGAMVDPRLLIGN-SVEASRELSSIPNLPPPPPPPPPPPPCV 180

Query: 181 SDRCDICFKVIFIILRINLFGFRENFRDEKTVIHIHLRFPTP-----KSIKLITHA---- 240
           SDRCDICFK      R+N      N   EK +I      P P       + L T++    
Sbjct: 181 SDRCDICFKK----KRVNF----SNTMKEKNIIGTAEPPPPPAVASFDFLGLNTNSAATD 240

Query: 241 IFLSSRFNQT--------EETLGNGGGGG--ENILMEYEFFPGKNGRGTEFKE------- 300
              S  FNQ          +  G GGGGG   + LMEYEFFP KNGRGTE +E       
Sbjct: 241 FDFSLNFNQEGAGVKQIHRKVAGKGGGGGGEGSTLMEYEFFPRKNGRGTELEERKRANEE 300

Query: 301 ----LEMPKEEEEEEFSFAEEAVDHGEASSCITTTYNTAIVNNNGSSSSGSSAVDLSLKL 314
                E  +EEEEEE   A +AVDHGE  SCITT+ +  I   NG + + S+ +DLSLKL
Sbjct: 301 LGLFAEEEEEEEEEEEEEAVQAVDHGE-GSCITTSCSDII---NGGTRN-STVLDLSLKL 331

[Close]

BLAST of MS021351 vs. ExPASy TrEMBL
Match: A0A0A0LDJ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G850670 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 7.8e-39
Identity = 146/327 (44.65%), Postives = 170/327 (51.99%), Query Frame = 0


Query: 24  EPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERLRLQERWKKMTEMPPPPVHAHH-FA 83
           EP KTR  R  K  PK P  KKPPQRGLGVAQLERLRLQE WK +TE+ PP    H+   
Sbjct: 16  EPPKTRAGR--KPGPKNPNQKKPPQRGLGVAQLERLRLQENWKTVTEISPPTFLLHNPLP 75

Query: 84  NIPGLHFSAV------DDCTG-EGGGLVFQGMGNVGGFVAGAGGFTVVEPYTHGGGALDP 143
           N P LHF          DC G +  G V Q +GN GGF+  +G                 
Sbjct: 76  NFP-LHFPPAPAPILHTDCIGFDHHGFVVQRIGNNGGFLPASG----------------- 135

Query: 144 RVLIGSYGEEDLRELSSIPKMPMPCVSDRCDICFKVIFIILRINLFGFRENFRDEKTVI- 203
            VLIG+   E  RELSSIPK+P+ C SDRCD CFK      R+N      N   EK +I 
Sbjct: 136 -VLIGNTSVEASRELSSIPKLPLACDSDRCDHCFKK----KRVNY----SNRMKEKNIIV 195

Query: 204 -------HIHLRFPTPKSIKLITHAIFLSSRFNQTEETL--------------GNGGGGG 263
                     L   T  S +L TH    +   + T   L              G GG GG
Sbjct: 196 GAAETPSFDFLGLSTNSSAELNTHTHTHTVMNHHTNSDLDLDYDLSFNLKQGRGGGGDGG 255

Query: 264 E-NILMEYEFFPGKNGRGTEFKELEMPKE------EEEEEFSFAEEAVDHGEASSCITTT 314
           E + LMEYEFFP KNGRGTE +EL+MPKE      EE EE      A+DHGE  SCITT+
Sbjct: 256 EGSKLMEYEFFPRKNGRGTEIEELKMPKEELSLFREENEEEEEEVLAMDHGE-GSCITTS 308

[Close]

BLAST of MS021351 vs. ExPASy TrEMBL
Match: A0A1S3BZ18 (uncharacterized protein LOC103494987 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494987 PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 3.0e-38
Identity = 148/354 (41.81%), Postives = 178/354 (50.28%), Query Frame = 0


Query: 1   MATPMLLMAAQKSNANASQETKPEPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERLR 60
           MATP+     Q+  +N S   KP   +       K  PK P  KKPPQRGLGVAQLERLR
Sbjct: 1   MATPL-----QQQTSNTSHPPKPRAGR-------KPGPKNPNQKKPPQRGLGVAQLERLR 60

Query: 61  LQERWKKMTEMPPPPVHAHH-FANIPGLHFSAV------DDCTGEGG---------GLVF 120
           LQE WK +TE+ PP    H+   N P LHF          DC   G          G V 
Sbjct: 61  LQENWKTVTEISPPTFLLHNTLPNFP-LHFPPAPPPILHTDCIAAGAGAVLGFDHHGFVV 120

Query: 121 QGMGNVGGFVAGAGGFTVVEPYTHGGGALDPRVLIGSYGEEDLRELSSIPKMPMPCVSDR 180
           Q +GN GGF+   G                  VLIG+   E  RELSSIPK+P+ C SDR
Sbjct: 121 QRIGNNGGFLPAGG------------------VLIGNTSVEASRELSSIPKLPLACDSDR 180

Query: 181 CDICFKVIFIILRINLFGFRENFRDEKTVIHIHLRFP--------TPKSIKLITHAIF-- 240
           CD CFK      R+N      N   EK +I      P        T  + +L TH++   
Sbjct: 181 CDHCFK-----KRVNF----SNRMKEKNIIAAAAETPSFDFLGLGTNSTAELNTHSVMNH 240

Query: 241 -------LSSRFN-QTEETLGNGGGGGE-NILMEYEFFPGKNGRGTEFKELEMPKE---- 300
                  L   F+   ++  G GG GGE + LMEYEFFP KNGRGTE +EL+MPKE    
Sbjct: 241 HTDSGWDLDYDFSLNLKQVRGGGGDGGEGSKLMEYEFFPRKNGRGTEIEELKMPKEELSL 300

Query: 301 --EEEEEFSFAEEAVDHGEASSCITTTYNTAIVNNNGSSSSGSSAVDLSLKLSF 314
             EE EE      A+DHGE  SCITT+ N  I   NG + + S+A+DLSLKLSF
Sbjct: 301 FREENEEEEEEVLAMDHGE-GSCITTSCNDII---NGGTRN-STALDLSLKLSF 309

[Close]

BLAST of MS021351 vs. ExPASy TrEMBL
Match: A0A5A7T067 (Protein SPOROCYTELESS OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G001020 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 3.9e-38
Identity = 148/354 (41.81%), Postives = 178/354 (50.28%), Query Frame = 0


Query: 1   MATPMLLMAAQKSNANASQETKPEPTKTRPSRSSKSAPKAPAHKKPPQRGLGVAQLERLR 60
           MATP+     Q+  +N S   KP   +       K  PK P  KKPPQRGLGVAQLERLR
Sbjct: 1   MATPL-----QQQTSNTSHPPKPRAGR-------KPGPKNPNQKKPPQRGLGVAQLERLR 60

Query: 61  LQERWKKMTEMPPPPVHAHH-FANIPGLHFSAV------DDCTGEGG---------GLVF 120
           LQE WK +TE+ PP    H+   N P LHF          DC   G          G V 
Sbjct: 61  LQENWKTVTEISPPTFLLHNTLPNFP-LHFPPAPPPILHTDCIAAGAGAVLGFDHHGFVV 120

Query: 121 QGMGNVGGFVAGAGGFTVVEPYTHGGGALDPRVLIGSYGEEDLRELSSIPKMPMPCVSDR 180
           Q +GN GGF+   G                  VLIG+   E  RELSSIPK+P+ C SDR
Sbjct: 121 QRIGNNGGFLPAGG------------------VLIGNTSVEASRELSSIPKLPLACDSDR 180

Query: 181 CDICFKVIFIILRINLFGFRENFRDEKTVIHIHLRFP--------TPKSIKLITHAIF-- 240
           CD CFK      R+N      N   EK +I      P        T  + +L TH++   
Sbjct: 181 CDHCFKK----KRVNF----SNRMKEKNIIAAAAETPSFDFLGLGTNSTAELNTHSVMNH 240

Query: 241 -------LSSRFN-QTEETLGNGGGGGE-NILMEYEFFPGKNGRGTEFKELEMPKE---- 300
                  L   F+   ++  G GG GGE + LMEYEFFP KNGRGTE +EL+MPKE    
Sbjct: 241 HTDSGWDLDYDFSLNLKQVRGGGGDGGEGSKLMEYEFFPRKNGRGTEIEELKMPKEELSL 300

Query: 301 --EEEEEFSFAEEAVDHGEASSCITTTYNTAIVNNNGSSSSGSSAVDLSLKLSF 314
             EE EE      A+DHGE  SCITT+ N  I   NG + + S+A+DLSLKLSF
Sbjct: 301 FREENEEEEEEVLAMDHGE-GSCITTSCNDII---NGGTRN-STALDLSLKLSF 310

The following BLAST results are available for this feature:

BLAST of MS021351 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022975733.1	1.6e-46	46.86	protein SPOROCYTELESS [Cucurbita maxima]	[more]
XP_022936226.1	2.0e-44	46.13	protein virilizer homolog [Cucurbita moschata]	[more]
KAG7024875.1	1.7e-43	45.86	hypothetical protein SDJN02_13694, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6591999.1	4.8e-43	45.21	hypothetical protein SDJN03_14345, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038900102.1	5.0e-40	43.44	protein SPOROCYTELESS-like [Benincasa hispida]	[more]

BLAST of MS021351 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS021351 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IK53	7.8e-47	46.86	protein SPOROCYTELESS OS=Cucurbita maxima OX=3661 GN=LOC111475949 PE=4 SV=1	[more]
A0A6J1F6X7	9.5e-45	46.13	protein virilizer homolog OS=Cucurbita moschata OX=3662 GN=LOC111442900 PE=4 SV=...	[more]
A0A0A0LDJ3	7.8e-39	44.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G850670 PE=4 SV=1	[more]
A0A1S3BZ18	3.0e-38	41.81	uncharacterized protein LOC103494987 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5A7T067	3.9e-38	41.81	Protein SPOROCYTELESS OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11...	[more]

BLAST of MS021351 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014855	Plant transcription factor NOZZLE	PFAM	PF08744	NOZZLE	coord: 7..170 e-value: 1.7E-8 score: 34.4
IPR014855	Plant transcription factor NOZZLE	PANTHER	PTHR33388:SF2	PROTEIN SPOROCYTELESS	coord: 1..313
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 294..313
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 9..35
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..55
IPR040356	SPEAR family	PANTHER	PTHR33388	OS01G0212500 PROTEIN	coord: 1..313

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021351.1	MS021351.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003700	DNA-binding transcription factor activity