MS021271 (gene) Bitter gourd (TR) v1

Overview


Name	MS021271
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	E3 SUMO-protein ligase MMS21
Location	scaffold358: 636385 .. 639354 (-)
RNA-Seq Expression	MS021271
Synteny	MS021271

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCGACTTCTGATTCCCGTTCAAGTGCCGTTTCGGGCAGAATCAAATCCGCTGCCACTATGATGCACTCCGAGAATCAGTCCCTGCTTGCTGTAAATTGCTTAATACTACTCGCTATTTATATGATTACTTCTAATTTGTTGCCGTTTTGGTTCTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNAAATTTGGTTTGGGAAATGCGGCGCTGAGAAGAAAAGGAAGTTGTTGGTGCTAAATTAATGTTGTTTTCAGCTTTAAGATCTCGATTTTTTTGTATAGAGAGTCGGTTGTAATGCTGTATTCCTTTAATTCTGGCGCTGTTTCTCTGGATTTCTGAAACGTCGGGGTCTTGGGCGCGAGCTGAGAAGCTACAGGAAGATATCGTATGTATTTTCTCATAGAAAATCCCACCCAATAACCTGTTTTGTTCAGTTCGTGGTCTGATAAGACAATTAAGAGATTCGTCAATTAAATTTAAAGGGAAGATAAGAGAACGTTCTTCTGATGTACTCCTCAGTCATCTCGCACTTGAGTTGGTTAATCTACTACAATCCTGTCTTGTTCAAAAGAACGATTTATTTGGATATTAGGCTAATGATGATGGGTTATCTGTTGATTTTTGTGATGTGATTCAACTTTTCTGTTTTAATTTCAGGAAATACGCAAGTTGTTGATTATGATGAAGGAAATTGGGATGGATTTGGAGAGGGACAATCAGTCGACGATGGTATTATGGATAATGAACCCTTGTTTCAATTTACTTTGGAAGAGTGTTTTCAGGATAAGATCTTGTAATAAAATGTTAGAAGCAAGAACACAAATTTCAAAATTTTTTTTTCTAGGACTCGCATCATAAGGCTTTATTTTAACATCTTTGTTGCCTTTAAAAAAAAAAAAATTAATTGAAGAAACGACGTGGGTAAAGGAAAACAAGAAGCATGTGCAGTGTACTATGTACAATCTGCTTTAGAACTTGCAATATAAACCAGCCACTTTGTCAAGCCGTCAAAATCAAATATTTTAAATGTTTATTTCTGTTCGGTCATTTTGCTTGTTTAGTAACCGACATATTGCTTTTTGATGGACTTTCTGTGGATGCCTCGATTTGAAGAAGATTTATTTCAACGGATACCTTGCTTAAAAATTATGGTCTTGGTTTCCCTAGGTGTACGGTTAATTAATTAGAATGCAGCAAATACTTTTTGGTGGATTTTCTTCTTCTGGCTATTAAATCCGGTCTAAAGATTATTGAGAATCTTTGTTGTTTGGAAGTCTTAACCAATTATTTACTTGGTATTTGAAGGTCAAGAAGCTTGAAAATGCTGTTGTTGAGCTGTTGAGTACTTACGAAAACTGTGACAACTTTTCATCTGCGATTCAGTCGGTTGGAAATATATATGAACCAAGAGAAGAGGTATTTAAATCTCTTTCCATGTTGAATTTCCCACGATATATTCGACATTCGTTTGTTAATTTTCATAGTGCTGGTTCCTTCGTTTCCATACCTAATGATAGTTTATGTTGATCCTGTAGTTAACAGATTTTGAGAAACTACTTGACGATGAAGTTGCAAAAGTCAGTGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAAGTTTAGAGAAGCTATTTGGGTATGCATCTTCATCTTATTTTCTATGCTCCGGGACAGGCAATTGGCACCTTGAAAGACAGTAGATTAGAAAGCCAGTGGAAAGTATTTCCATTTTATTAACTGATTATAGGGATTGCGATACCTCTTTGGCACTGATTACACATGGTAGCAACAAAAAAGTTGTCAAATCTGTGCAAATAATCATCCTAAAACTGTGAAAACGCAACAGAAAGTACATGATAAGTAAACAAGAGAAACTACTTTGTTTGTTTGCAATTAAAGTACATGAGAAACAATTTTATTATTCTTCCAATTGTGTAGATATTTATCTTTCCAGTGCTTTGGCTTATCTCCCGATATTCCTGCAGAATGTACATCATGCAGGGCAACCAATGCCAGGTGAAGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATTTATTGAATGTCACTTGCCCATTAAGTGGAAAGCCTGTCACTGAATTGGCACAACCTGTTCGCAGGTATATTGCAACGAAAGATTTTTTCATGAAGAACGAAGAATTAGATATATTTCTGTTACTGTTGAAAAATAAGTCTGCTCATCTATTCACTCTGTACTAACAGCATGGATGGTTACATGCTTGTGTTATTCTCTCTGGTTACATTTATCAAGTGGGTGCCTGAAAATGGAATGAGCTAGACTTTCACTTGTGATTTTTTTCCCTCGTAATAAATTGACAACCACCCTGAGTTAGACTTAGACTGCTGACATTGGGGATGTGAGAAAAAGTAAAGAGAATTTAGAGGAATGTGTTTAAATCACATGTTGGCAAGATGACAAATTTCTTAACAACTAAATAATGTAGGATCAAACAGTTATCTTTGCCAGCTTTTGCATGATAAATGGGTCTGGTACTCGCTTTTTGAGTTGATCTAATAGATCTTGAACTTTAAAAGTGTCTAATAGAATCTGAACTTTTGATTCTATCTCTACCTTTTACAATTTGTAGTATGGAATGCAAGCATGTGTATGAAAAGGAGGCTGTGATGCAGTACCTAAGATCTAAGAATTCTCGAGCTCAATGCCCTGTAGCAGGTATCCAGGTCTTCTACATGTAATGTAGTACATACAAAATGTATAGATTGCTTTTCTAGTTTTCTCACTTGAACTCTTTGTCCTTGTAGCCTGTCCTAAGATGTTGCAGCCAGAAAAGGTCACGCCTGATCCGTTCTTACTGATTGAAATTGATGAACTGAGAAAAACGTCTAGGCACTCTGTGAGAATACAAGATTTTACAGAGCTTGATGCAGAA

mRNA sequence

ATGGCGTCGACTTCTGATTCCCGTTCAAGTGCCGTTTCGGGCAGAATCAAATCCGCTGCCACTATGATGCACTCCGAGAATCAGTCCCTGCTTGCTGAAATACGCAAGTTGTTGATTATGATGAAGGAAATTGGGATGGATTTGGAGAGGGACAATCAGTCGACGATGGTCAAGAAGCTTGAAAATGCTGTTGTTGAGCTGTTGAGTACTTACGAAAACTGTGACAACTTTTCATCTGCGATTCAGTCGGTTGGAAATATATATGAACCAAGAGAAGAGTTAACAGATTTTGAGAAACTACTTGACGATGAAGTTGCAAAAGTCAGTGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAAGTTTAGAGAAGCTATTTGGAATGTACATCATGCAGGGCAACCAATGCCAGGTGAAGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATTTATTGAATGTCACTTGCCCATTAAGTGGAAAGCCTGTCACTGAATTGGCACAACCTGTTCGCAGTATGGAATGCAAGCATGTGTATGAAAAGGAGGCTGTGATGCAGTACCTAAGATCTAAGAATTCTCGAGCTCAATGCCCTGTAGCAGCCTGTCCTAAGATGTTGCAGCCAGAAAAGGTCACGCCTGATCCGTTCTTACTGATTGAAATTGATGAACTGAGAAAAACGTCTAGGCACTCTGTGAGAATACAAGATTTTACAGAGCTTGATGCAGAA

Coding sequence (CDS)

ATGGCGTCGACTTCTGATTCCCGTTCAAGTGCCGTTTCGGGCAGAATCAAATCCGCTGCCACTATGATGCACTCCGAGAATCAGTCCCTGCTTGCTGAAATACGCAAGTTGTTGATTATGATGAAGGAAATTGGGATGGATTTGGAGAGGGACAATCAGTCGACGATGGTCAAGAAGCTTGAAAATGCTGTTGTTGAGCTGTTGAGTACTTACGAAAACTGTGACAACTTTTCATCTGCGATTCAGTCGGTTGGAAATATATATGAACCAAGAGAAGAGTTAACAGATTTTGAGAAACTACTTGACGATGAAGTTGCAAAAGTCAGTGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAAGTTTAGAGAAGCTATTTGGAATGTACATCATGCAGGGCAACCAATGCCAGGTGAAGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATTTATTGAATGTCACTTGCCCATTAAGTGGAAAGCCTGTCACTGAATTGGCACAACCTGTTCGCAGTATGGAATGCAAGCATGTGTATGAAAAGGAGGCTGTGATGCAGTACCTAAGATCTAAGAATTCTCGAGCTCAATGCCCTGTAGCAGCCTGTCCTAAGATGTTGCAGCCAGAAAAGGTCACGCCTGATCCGTTCTTACTGATTGAAATTGATGAACTGAGAAAAACGTCTAGGCACTCTGTGAGAATACAAGATTTTACAGAGCTTGATGCAGAA

Protein sequence

MASTSDSRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQDFTELDAE

Homology

[Close]

BLAST of MS021271 vs. NCBI nr
Match: XP_022139610.1 (E3 SUMO-protein ligase MMS21 isoform X1 [Momordica charantia])

HSP 1 Score: 478.8 bits (1231), Expect = 2.9e-131
Identity = 247/248 (99.60%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MASTSDSRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTSD+RSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS
Sbjct: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC
Sbjct: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ
Sbjct: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTELDAE
Sbjct: 241 DFTELDAE 248

[Close]

BLAST of MS021271 vs. NCBI nr
Match: XP_022941288.1 (E3 SUMO-protein ligase MMS21 [Cucurbita moschata])

HSP 1 Score: 423.7 bits (1088), Expect = 1.1e-114
Identity = 216/248 (87.10%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASTSDSRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTS+S S+ VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG++LERDNQS MVK+L
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVELERDNQSRMVKEL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           EN+VVELL TYE C NFSSAIQSVGNIYEP+EELTDFEKLLDDEVAKVSENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTE+DA+
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of MS021271 vs. NCBI nr
Match: XP_023525588.1 (E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-protein ligase MMS21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 423.3 bits (1087), Expect = 1.4e-114
Identity = 216/248 (87.10%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASTSDSRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTS+S S+ VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG+DLERDNQS MVK+L
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           EN+VVELL TYE C NFSSAIQSVGNIYEP+EELTDFEKLLD+EVAKVSENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTE+DA+
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of MS021271 vs. NCBI nr
Match: KAG6607898.1 (E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 421.4 bits (1082), Expect = 5.4e-114
Identity = 215/248 (86.69%), Postives = 233/248 (93.95%), Query Frame = 0


Query: 1   MASTSDSRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTS+S S+ VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG+DLERDNQS MVK+L
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           EN+VVELL TYE C NFSSAIQSVGNIYEP+EELTDFEKLLD+EVAKVSENSSSNL NHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLHNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTE+DA+
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of MS021271 vs. NCBI nr
Match: XP_022981951.1 (E3 SUMO-protein ligase MMS21 [Cucurbita maxima])

HSP 1 Score: 420.2 bits (1079), Expect = 1.2e-113
Identity = 217/250 (86.80%), Postives = 236/250 (94.40%), Query Frame = 0


Query: 1   MASTSDSRSSA--VSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVK 60
           MASTS+S S++  VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG+DLERDNQS MVK
Sbjct: 1   MASTSNSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK 60

Query: 61  KLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQN 120
           +LEN+VVELLSTYE C NFSSAIQSVGNIYEP+EELTDFEKLLD+EVAKVSENSSSNLQN
Sbjct: 61  ELENSVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN 120

Query: 121 HSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSM 180
           HSIIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS 
Sbjct: 121 HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST 180

Query: 181 ECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVR 240
           ECKHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVR
Sbjct: 181 ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR 240

Query: 241 IQDFTELDAE 249
           IQDFTE+DA+
Sbjct: 241 IQDFTEVDAD 250

[Close]

BLAST of MS021271 vs. ExPASy Swiss-Prot
Match: Q8GYH7 (E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 3.2e-69
Identity = 137/243 (56.38%), Postives = 178/243 (73.25%), Query Frame = 0


Query: 5   SDSRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKLENAV 64
           S S S  V+GRI++A+ ++ S+N S LA+IRK + MMK I + LE++NQ+  VK LEN+V
Sbjct: 3   SASSSDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDLENSV 62

Query: 65  VELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHSIIRK 124
            ELL  + +C++ S+AIQSV N Y+P E+LTDF+KLLDDE  K+    SS  QN  ++R+
Sbjct: 63  AELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDHLMRQ 122

Query: 125 FREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMECKHVY 184
           FREA+WNVHHAG+PMPG++ EDIVMTSTQC LLN+TCPLSGKPVTELA PVRSM+C+HVY
Sbjct: 123 FREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDCRHVY 182

Query: 185 EKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVR---IQD 244
           EK  ++ Y+   N  A CPVA C   LQ  KV  D  L  EI+E+R  ++ S R   I+D
Sbjct: 183 EKSVILHYI-VNNPNANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAEVIED 242

[Close]

BLAST of MS021271 vs. ExPASy TrEMBL
Match: A0A6J1CCS6 (E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010470 PE=3 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.4e-131
Identity = 247/248 (99.60%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MASTSDSRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTSD+RSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS
Sbjct: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC
Sbjct: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ
Sbjct: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTELDAE
Sbjct: 241 DFTELDAE 248

[Close]

BLAST of MS021271 vs. ExPASy TrEMBL
Match: A0A6J1FRP6 (E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.3e-115
Identity = 216/248 (87.10%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASTSDSRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTS+S S+ VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG++LERDNQS MVK+L
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVELERDNQSRMVKEL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           EN+VVELL TYE C NFSSAIQSVGNIYEP+EELTDFEKLLDDEVAKVSENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTE+DA+
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of MS021271 vs. ExPASy TrEMBL
Match: A0A6J1IXZ2 (E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 5.8e-114
Identity = 217/250 (86.80%), Postives = 236/250 (94.40%), Query Frame = 0


Query: 1   MASTSDSRSSA--VSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVK 60
           MASTS+S S++  VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG+DLERDNQS MVK
Sbjct: 1   MASTSNSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK 60

Query: 61  KLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQN 120
           +LEN+VVELLSTYE C NFSSAIQSVGNIYEP+EELTDFEKLLD+EVAKVSENSSSNLQN
Sbjct: 61  ELENSVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN 120

Query: 121 HSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSM 180
           HSIIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS 
Sbjct: 121 HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST 180

Query: 181 ECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVR 240
           ECKHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVR
Sbjct: 181 ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR 240

Query: 241 IQDFTELDAE 249
           IQDFTE+DA+
Sbjct: 241 IQDFTEVDAD 250

[Close]

BLAST of MS021271 vs. ExPASy TrEMBL
Match: A0A6J1CD70 (E3 SUMO-protein ligase MMS21 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010470 PE=3 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 4.9e-113
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 40  MMKEIGMDLERDNQSTMVKKLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEK 99
           MMKEIGMDLERDNQSTMVKKLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEK
Sbjct: 1   MMKEIGMDLERDNQSTMVKKLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEK 60

Query: 100 LLDDEVAKVSENSSSNLQNHSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNV 159
           LLDDEVAKVSENSSSNLQNHSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNV
Sbjct: 61  LLDDEVAKVSENSSSNLQNHSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNV 120

Query: 160 TCPLSGKPVTELAQPVRSMECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPD 219
           TCPLSGKPVTELAQPVRSMECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPD
Sbjct: 121 TCPLSGKPVTELAQPVRSMECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPD 180

Query: 220 PFLLIEIDELRKTSRHSVRIQDFTELDAE 249
           PFLLIEIDELRKTSRHSVRIQDFTELDAE
Sbjct: 181 PFLLIEIDELRKTSRHSVRIQDFTELDAE 209

[Close]

BLAST of MS021271 vs. ExPASy TrEMBL
Match: A0A5D3DWX9 (E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001530 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.0e-110
Identity = 207/248 (83.47%), Postives = 227/248 (91.53%), Query Frame = 0


Query: 1   MASTSDSRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MAS SDSRS+ V+GRIKSAAT+MHSENQSLLAE+RK LIMMKEIG+DLE++NQ+ MVK+L
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLLAELRKTLIMMKEIGVDLEKENQNKMVKEL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           E ++VELLS YENC+NFSSAIQSVGN YEP+EELTDFEKLLDDEVAKVS NSSSN  NH 
Sbjct: 61  EKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSVNSSSNFANHP 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIR+FREAIWNVHHAGQPM GEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSAEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KH+YEK A+MQYL SK SRAQCPVAACPKMLQP+KV  DPFL IEIDELRK SRHS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSERIQ 240

Query: 241 DFTELDAE 249
           DFTELDA+
Sbjct: 241 DFTELDAD 248

[Close]

BLAST of MS021271 vs. TAIR 10
Match: AT3G15150.1 (RING/U-box superfamily protein )

HSP 1 Score: 263.1 bits (671), Expect = 2.3e-70
Identity = 137/243 (56.38%), Postives = 178/243 (73.25%), Query Frame = 0


Query: 5   SDSRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKLENAV 64
           S S S  V+GRI++A+ ++ S+N S LA+IRK + MMK I + LE++NQ+  VK LEN+V
Sbjct: 3   SASSSDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDLENSV 62

Query: 65  VELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHSIIRK 124
            ELL  + +C++ S+AIQSV N Y+P E+LTDF+KLLDDE  K+    SS  QN  ++R+
Sbjct: 63  AELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDHLMRQ 122

Query: 125 FREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMECKHVY 184
           FREA+WNVHHAG+PMPG++ EDIVMTSTQC LLN+TCPLSGKPVTELA PVRSM+C+HVY
Sbjct: 123 FREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDCRHVY 182

Query: 185 EKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVR---IQD 244
           EK  ++ Y+   N  A CPVA C   LQ  KV  D  L  EI+E+R  ++ S R   I+D
Sbjct: 183 EKSVILHYI-VNNPNANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAEVIED 242

The following BLAST results are available for this feature:

BLAST of MS021271 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139610.1	2.9e-131	99.60	E3 SUMO-protein ligase MMS21 isoform X1 [Momordica charantia]	[more]
XP_022941288.1	1.1e-114	87.10	E3 SUMO-protein ligase MMS21 [Cucurbita moschata]	[more]
XP_023525588.1	1.4e-114	87.10	E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-...	[more]
KAG6607898.1	5.4e-114	86.69	E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022981951.1	1.2e-113	86.80	E3 SUMO-protein ligase MMS21 [Cucurbita maxima]	[more]

BLAST of MS021271 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYH7	3.2e-69	56.38	E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1	[more]

BLAST of MS021271 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CCS6	1.4e-131	99.60	E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1FRP6	5.3e-115	87.10	E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 ...	[more]
A0A6J1IXZ2	5.8e-114	86.80	E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV...	[more]
A0A6J1CD70	4.9e-113	100.00	E3 SUMO-protein ligase MMS21 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5D3DWX9	1.0e-110	83.47	E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of MS021271 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15150.1	2.3e-70	56.38	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004181	Zinc finger, MIZ-type	PFAM	PF11789	zf-Nse	coord: 145..207 e-value: 5.9E-15 score: 54.9
IPR004181	Zinc finger, MIZ-type	CDD	cd16651	SPL-RING_NSE2	coord: 160..228 e-value: 6.61266E-23 score: 86.5296
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 153..239 e-value: 1.3E-18 score: 69.0
IPR026846	E3 SUMO-protein ligase Nse2 (Mms21)	PANTHER	PTHR21330	UNCHARACTERIZED	coord: 8..235
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 159..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021271.1	MS021271.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000724	double-strand break repair via homologous recombination
cellular_component	GO:0030915	Smc5-Smc6 complex
molecular_function	GO:0019789	SUMO transferase activity
molecular_function	GO:0008270	zinc ion binding