MS021268 (gene) Bitter gourd (TR) v1

BLAST of MS021268 vs. NCBI nr
Match: XP_022139557.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X8 [Momordica charantia])

HSP 1 Score: 857.4 bits (2214), Expect = 5.2e-245
Identity = 424/470 (90.21%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   KRTLAPSHTTPAIDLELDQQGQGTFSQRSNHSMVTAFRSSTNPEVQHQPEHANGAILYAM 60
           KRTLAPSHTT AIDLELDQQGQGTFSQRSNHSMVT FRSSTNPEVQHQPEHANGAILYAM
Sbjct: 31  KRTLAPSHTTSAIDLELDQQGQGTFSQRSNHSMVTGFRSSTNPEVQHQPEHANGAILYAM 90

Query: 61  SQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 120
           SQYSGSMIQRAQDM VATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV
Sbjct: 91  SQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 150

Query: 121 GSPYKRRFAEPVPVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 180
           GSPYKRRFAEPV VNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL
Sbjct: 151 GSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 210

Query: 181 WLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 240
           WLDQRFNCNFEDRGAWNWNHAPAGFP+HGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ
Sbjct: 211 WLDQRFNCNFEDRGAWNWNHAPAGFPVHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 270

Query: 241 HPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 300
           HPPSTLIQHPSYRIPLPQ HLQGQRGQNI LNPPVAAVTQRFPLSSAYGIMHQQFLELEP
Sbjct: 271 HPPSTLIQHPSYRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 330

Query: 301 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG------------------------- 360
           RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG                         
Sbjct: 331 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEGEAGVPGAQASASASDSNDIGDAYRD 390

Query: 361 -----------ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEET 420
                      ELLALEE+IGYVGTGLSEEIITSQLKTRISMPS RDANLEE ASRNEET
Sbjct: 391 MRLDIEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEET 450

Query: 421 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 435
           NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR
Sbjct: 451 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 500

BLAST of MS021268 vs. NCBI nr
Match: XP_022139556.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X7 [Momordica charantia])

HSP 1 Score: 857.4 bits (2214), Expect = 5.2e-245
Identity = 424/470 (90.21%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   KRTLAPSHTTPAIDLELDQQGQGTFSQRSNHSMVTAFRSSTNPEVQHQPEHANGAILYAM 60
           KRTLAPSHTT AIDLELDQQGQGTFSQRSNHSMVT FRSSTNPEVQHQPEHANGAILYAM
Sbjct: 36  KRTLAPSHTTSAIDLELDQQGQGTFSQRSNHSMVTGFRSSTNPEVQHQPEHANGAILYAM 95

Query: 61  SQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 120
           SQYSGSMIQRAQDM VATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV
Sbjct: 96  SQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 155

Query: 121 GSPYKRRFAEPVPVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 180
           GSPYKRRFAEPV VNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL
Sbjct: 156 GSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 215

Query: 181 WLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 240
           WLDQRFNCNFEDRGAWNWNHAPAGFP+HGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ
Sbjct: 216 WLDQRFNCNFEDRGAWNWNHAPAGFPVHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 275

Query: 241 HPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 300
           HPPSTLIQHPSYRIPLPQ HLQGQRGQNI LNPPVAAVTQRFPLSSAYGIMHQQFLELEP
Sbjct: 276 HPPSTLIQHPSYRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 335

Query: 301 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG------------------------- 360
           RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG                         
Sbjct: 336 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEGEAGVPGAQASASASDSNDIGDAYRD 395

Query: 361 -----------ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEET 420
                      ELLALEE+IGYVGTGLSEEIITSQLKTRISMPS RDANLEE ASRNEET
Sbjct: 396 MRLDIEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEET 455

Query: 421 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 435
           NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR
Sbjct: 456 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 505

BLAST of MS021268 vs. NCBI nr
Match: XP_022139559.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X10 [Momordica charantia] >XP_022139560.1 probable E3 ubiquitin-protein ligase ZFP1 isoform X10 [Momordica charantia])

HSP 1 Score: 857.4 bits (2214), Expect = 5.2e-245
Identity = 424/470 (90.21%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   KRTLAPSHTTPAIDLELDQQGQGTFSQRSNHSMVTAFRSSTNPEVQHQPEHANGAILYAM 60
           KRTLAPSHTT AIDLELDQQGQGTFSQRSNHSMVT FRSSTNPEVQHQPEHANGAILYAM
Sbjct: 5   KRTLAPSHTTSAIDLELDQQGQGTFSQRSNHSMVTGFRSSTNPEVQHQPEHANGAILYAM 64

Query: 61  SQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 120
           SQYSGSMIQRAQDM VATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV
Sbjct: 65  SQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 124

Query: 121 GSPYKRRFAEPVPVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 180
           GSPYKRRFAEPV VNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL
Sbjct: 125 GSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 184

Query: 181 WLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 240
           WLDQRFNCNFEDRGAWNWNHAPAGFP+HGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ
Sbjct: 185 WLDQRFNCNFEDRGAWNWNHAPAGFPVHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 244

Query: 241 HPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 300
           HPPSTLIQHPSYRIPLPQ HLQGQRGQNI LNPPVAAVTQRFPLSSAYGIMHQQFLELEP
Sbjct: 245 HPPSTLIQHPSYRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 304

Query: 301 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG------------------------- 360
           RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG                         
Sbjct: 305 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEGEAGVPGAQASASASDSNDIGDAYRD 364

Query: 361 -----------ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEET 420
                      ELLALEE+IGYVGTGLSEEIITSQLKTRISMPS RDANLEE ASRNEET
Sbjct: 365 MRLDIEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEET 424

Query: 421 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 435
           NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR
Sbjct: 425 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 474

BLAST of MS021268 vs. NCBI nr
Match: XP_022139553.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X5 [Momordica charantia])

HSP 1 Score: 857.4 bits (2214), Expect = 5.2e-245
Identity = 424/470 (90.21%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   KRTLAPSHTTPAIDLELDQQGQGTFSQRSNHSMVTAFRSSTNPEVQHQPEHANGAILYAM 60
           KRTLAPSHTT AIDLELDQQGQGTFSQRSNHSMVT FRSSTNPEVQHQPEHANGAILYAM
Sbjct: 43  KRTLAPSHTTSAIDLELDQQGQGTFSQRSNHSMVTGFRSSTNPEVQHQPEHANGAILYAM 102

Query: 61  SQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 120
           SQYSGSMIQRAQDM VATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV
Sbjct: 103 SQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 162

Query: 121 GSPYKRRFAEPVPVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 180
           GSPYKRRFAEPV VNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL
Sbjct: 163 GSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 222

Query: 181 WLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 240
           WLDQRFNCNFEDRGAWNWNHAPAGFP+HGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ
Sbjct: 223 WLDQRFNCNFEDRGAWNWNHAPAGFPVHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 282

Query: 241 HPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 300
           HPPSTLIQHPSYRIPLPQ HLQGQRGQNI LNPPVAAVTQRFPLSSAYGIMHQQFLELEP
Sbjct: 283 HPPSTLIQHPSYRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 342

Query: 301 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG------------------------- 360
           RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG                         
Sbjct: 343 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEGEAGVPGAQASASASDSNDIGDAYRD 402

Query: 361 -----------ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEET 420
                      ELLALEE+IGYVGTGLSEEIITSQLKTRISMPS RDANLEE ASRNEET
Sbjct: 403 MRLDIEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEET 462

Query: 421 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 435
           NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR
Sbjct: 463 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 512

BLAST of MS021268 vs. NCBI nr
Match: XP_022139551.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X3 [Momordica charantia])

HSP 1 Score: 857.4 bits (2214), Expect = 5.2e-245
Identity = 424/470 (90.21%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   KRTLAPSHTTPAIDLELDQQGQGTFSQRSNHSMVTAFRSSTNPEVQHQPEHANGAILYAM 60
           KRTLAPSHTT AIDLELDQQGQGTFSQRSNHSMVT FRSSTNPEVQHQPEHANGAILYAM
Sbjct: 72  KRTLAPSHTTSAIDLELDQQGQGTFSQRSNHSMVTGFRSSTNPEVQHQPEHANGAILYAM 131

Query: 61  SQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 120
           SQYSGSMIQRAQDM VATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV
Sbjct: 132 SQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 191

Query: 121 GSPYKRRFAEPVPVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 180
           GSPYKRRFAEPV VNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL
Sbjct: 192 GSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 251

Query: 181 WLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 240
           WLDQRFNCNFEDRGAWNWNHAPAGFP+HGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ
Sbjct: 252 WLDQRFNCNFEDRGAWNWNHAPAGFPVHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 311

Query: 241 HPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 300
           HPPSTLIQHPSYRIPLPQ HLQGQRGQNI LNPPVAAVTQRFPLSSAYGIMHQQFLELEP
Sbjct: 312 HPPSTLIQHPSYRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 371

Query: 301 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG------------------------- 360
           RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG                         
Sbjct: 372 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEGEAGVPGAQASASASDSNDIGDAYRD 431

Query: 361 -----------ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEET 420
                      ELLALEE+IGYVGTGLSEEIITSQLKTRISMPS RDANLEE ASRNEET
Sbjct: 432 MRLDIEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEET 491

Query: 421 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 435
           NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR
Sbjct: 492 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 541

BLAST of MS021268 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.8e-22
Identity = 100/338 (29.59%), Postives = 144/338 (42.60%), Query Frame = 0

Query: 133 PVNFQGPNSSASSESLNAP---FDPMFCHTRSHLARGEY------MVQHLQPASNALWLD 192
           P  +    S  S E  + P   F+ M  H  S    G Y      + Q  QP SN  W+ 
Sbjct: 193 PSEYHSQTSWPSMEGSSIPSNGFNLMGAHPES-AQHGNYAFPTSHISQCFQPTSNT-WIS 252

Query: 193 QRFNCNFEDRGAWNW----NHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCS--- 252
           Q  N   +    W +    N+AP  F      +  G +E  N     Y       C    
Sbjct: 253 QSANGIADGIPQWEYVNGMNNAPGRF------SRSGMTETVNGSFREYQNGPSTLCRGPL 312

Query: 253 PSF-QHP---PSTLIQHPSYRIPLPQTH----LQG--QRGQNISLNPPVAAV-------- 312
           P F QH       L+ H   + P  Q H    L G    G    L P +  +        
Sbjct: 313 PYFHQHAGMHAHNLLDHTQVQAPYQQCHNNPVLHGVNHSGNRFHLGPRIPVLFSNSERTF 372

Query: 313 -TQRFPLSSAYGIMHQQFLELEPRQTRDLPFNDRRVYNPHETMFRRHLTSRMRV--LQEG 372
                PL  A  + H+    L P     + F+  R+Y     +   H   R+ +  +   
Sbjct: 373 GPPHHPL-LANPVNHRNIRILPPEHATIMDFS--RLYEV-SNVVDEHRDMRLDIDSMTYE 432

Query: 373 ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEETNSCTICLDVIE 432
           ELLALEE+IG V TGL++  I  +LKT + +P G      + +  + E ++C IC +  +
Sbjct: 433 ELLALEEQIGDVNTGLAKSYIVEKLKTSLFVP-GSSCMSNKSSESSMENDACIICQEEYQ 492

Query: 433 DGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALT 434
             + IG LDCGH YH DC+KQWL++KN+CP+CK+ AL+
Sbjct: 493 VKECIGTLDCGHRYHEDCIKQWLMVKNLCPICKTTALS 517

BLAST of MS021268 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 99.0 bits (245), Expect = 1.4e-19
Identity = 52/112 (46.43%), Postives = 68/112 (60.71%), Query Frame = 0

Query: 323 RHLTSRMRV--LQEGELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASR 382
           RH   R+ +  +   ELLALEERIG V TGLSEE +T  LK R       +A++EEE   
Sbjct: 562 RHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEE--- 621

Query: 383 NEETNSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEAL 433
                 C IC +   DG  +G LDCGH +H  C++QWL++KN CP+CK+ AL
Sbjct: 622 -----PCCICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNTAL 665

BLAST of MS021268 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.2e-18
Identity = 53/136 (38.97%), Postives = 71/136 (52.21%), Query Frame = 0

Query: 299 EPRQTRDLPFNDRRVYNPHETMFRRHLTSRMRV--LQEGELLALEERIGYVGTGLSEEII 358
           E  +  D    D  +Y     M  RH   R+ V  +   ELLAL ERIG V TGLSEE+I
Sbjct: 571 ENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELLALGERIGDVSTGLSEEVI 630

Query: 359 TSQLKTRISMPSGRDANLEEEASRNEETNSCTICLDVIEDGKKIGILDCGHYYHADCLKQ 418
              +K      S            ++    C IC +   +G  +G L CGH +H DC+KQ
Sbjct: 631 LKAMKQHKHTSSS-----PSSVELHQNIEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQ 690

Query: 419 WLLIKNVCPVCKSEAL 433
           W++IKN+CP+CK+EAL
Sbjct: 691 WVMIKNLCPICKTEAL 701

BLAST of MS021268 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 4.5e-18
Identity = 52/140 (37.14%), Postives = 77/140 (55.00%), Query Frame = 0

Query: 301 RQTRDLPFNDRRVYNP-----HETMFRRHLTSRMRV--LQEGELLALEERIGYVGTGLSE 360
           R+  +L F D  V++P        M  RH   R+ V  +   ELLAL ERIG V TGLSE
Sbjct: 534 RRGENLRFEDYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSYEELLALGERIGDVSTGLSE 593

Query: 361 EIITSQLKTRISMPSGRDANLEEEASRNEETNSCTICLDVIEDGKKIGILDCGHYYHADC 420
           E+I   +K        +  +    A  +++   C +C +   +G  +G L CGH +H  C
Sbjct: 594 EVILKVMK--------QHKHTSSAAGSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTAC 653

Query: 421 LKQWLLIKNVCPVCKSEALT 434
           +KQWL++KN+CP+CK+ AL+
Sbjct: 654 VKQWLMLKNLCPICKTVALS 665

BLAST of MS021268 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 9.5e-16
Identity = 39/97 (40.21%), Postives = 60/97 (61.86%), Query Frame = 0

Query: 336 ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEETNSCTICLDVIE 395
           ELLALEERIG V TG++EE I+++LK R           +      ++   C +C +   
Sbjct: 595 ELLALEERIGDVCTGVNEETISNRLKQR---------KYKSNTKSPQDAEPCCVCQEEYT 654

Query: 396 DGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEAL 433
           +G+ +G L+CGH +H+ C+K+WL  KN+CP+CK+  L
Sbjct: 655 EGEDMGTLECGHEFHSQCIKEWLKQKNLCPICKTTGL 682

BLAST of MS021268 vs. ExPASy TrEMBL
Match: A0A6J1CCM6 (probable E3 ubiquitin-protein ligase ZFP1 isoform X10 OS=Momordica charantia OX=3673 GN=LOC111010433 PE=4 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.5e-245
Identity = 424/470 (90.21%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   KRTLAPSHTTPAIDLELDQQGQGTFSQRSNHSMVTAFRSSTNPEVQHQPEHANGAILYAM 60
           KRTLAPSHTT AIDLELDQQGQGTFSQRSNHSMVT FRSSTNPEVQHQPEHANGAILYAM
Sbjct: 5   KRTLAPSHTTSAIDLELDQQGQGTFSQRSNHSMVTGFRSSTNPEVQHQPEHANGAILYAM 64

Query: 61  SQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 120
           SQYSGSMIQRAQDM VATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV
Sbjct: 65  SQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 124

Query: 121 GSPYKRRFAEPVPVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 180
           GSPYKRRFAEPV VNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL
Sbjct: 125 GSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 184

Query: 181 WLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 240
           WLDQRFNCNFEDRGAWNWNHAPAGFP+HGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ
Sbjct: 185 WLDQRFNCNFEDRGAWNWNHAPAGFPVHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 244

Query: 241 HPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 300
           HPPSTLIQHPSYRIPLPQ HLQGQRGQNI LNPPVAAVTQRFPLSSAYGIMHQQFLELEP
Sbjct: 245 HPPSTLIQHPSYRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 304

Query: 301 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG------------------------- 360
           RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG                         
Sbjct: 305 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEGEAGVPGAQASASASDSNDIGDAYRD 364

Query: 361 -----------ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEET 420
                      ELLALEE+IGYVGTGLSEEIITSQLKTRISMPS RDANLEE ASRNEET
Sbjct: 365 MRLDIEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEET 424

Query: 421 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 435
           NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR
Sbjct: 425 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 474

BLAST of MS021268 vs. ExPASy TrEMBL
Match: A0A6J1CD12 (probable E3 ubiquitin-protein ligase ZFP1 isoform X7 OS=Momordica charantia OX=3673 GN=LOC111010433 PE=4 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.5e-245
Identity = 424/470 (90.21%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   KRTLAPSHTTPAIDLELDQQGQGTFSQRSNHSMVTAFRSSTNPEVQHQPEHANGAILYAM 60
           KRTLAPSHTT AIDLELDQQGQGTFSQRSNHSMVT FRSSTNPEVQHQPEHANGAILYAM
Sbjct: 36  KRTLAPSHTTSAIDLELDQQGQGTFSQRSNHSMVTGFRSSTNPEVQHQPEHANGAILYAM 95

Query: 61  SQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 120
           SQYSGSMIQRAQDM VATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV
Sbjct: 96  SQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 155

Query: 121 GSPYKRRFAEPVPVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 180
           GSPYKRRFAEPV VNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL
Sbjct: 156 GSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 215

Query: 181 WLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 240
           WLDQRFNCNFEDRGAWNWNHAPAGFP+HGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ
Sbjct: 216 WLDQRFNCNFEDRGAWNWNHAPAGFPVHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 275

Query: 241 HPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 300
           HPPSTLIQHPSYRIPLPQ HLQGQRGQNI LNPPVAAVTQRFPLSSAYGIMHQQFLELEP
Sbjct: 276 HPPSTLIQHPSYRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 335

Query: 301 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG------------------------- 360
           RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG                         
Sbjct: 336 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEGEAGVPGAQASASASDSNDIGDAYRD 395

Query: 361 -----------ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEET 420
                      ELLALEE+IGYVGTGLSEEIITSQLKTRISMPS RDANLEE ASRNEET
Sbjct: 396 MRLDIEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEET 455

Query: 421 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 435
           NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR
Sbjct: 456 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 505

BLAST of MS021268 vs. ExPASy TrEMBL
Match: A0A6J1CE94 (probable E3 ubiquitin-protein ligase ZFP1 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111010433 PE=4 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.5e-245
Identity = 424/470 (90.21%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   KRTLAPSHTTPAIDLELDQQGQGTFSQRSNHSMVTAFRSSTNPEVQHQPEHANGAILYAM 60
           KRTLAPSHTT AIDLELDQQGQGTFSQRSNHSMVT FRSSTNPEVQHQPEHANGAILYAM
Sbjct: 54  KRTLAPSHTTSAIDLELDQQGQGTFSQRSNHSMVTGFRSSTNPEVQHQPEHANGAILYAM 113

Query: 61  SQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 120
           SQYSGSMIQRAQDM VATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV
Sbjct: 114 SQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 173

Query: 121 GSPYKRRFAEPVPVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 180
           GSPYKRRFAEPV VNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL
Sbjct: 174 GSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 233

Query: 181 WLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 240
           WLDQRFNCNFEDRGAWNWNHAPAGFP+HGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ
Sbjct: 234 WLDQRFNCNFEDRGAWNWNHAPAGFPVHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 293

Query: 241 HPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 300
           HPPSTLIQHPSYRIPLPQ HLQGQRGQNI LNPPVAAVTQRFPLSSAYGIMHQQFLELEP
Sbjct: 294 HPPSTLIQHPSYRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 353

Query: 301 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG------------------------- 360
           RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG                         
Sbjct: 354 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEGEAGVPGAQASASASDSNDIGDAYRD 413

Query: 361 -----------ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEET 420
                      ELLALEE+IGYVGTGLSEEIITSQLKTRISMPS RDANLEE ASRNEET
Sbjct: 414 MRLDIEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEET 473

Query: 421 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 435
           NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR
Sbjct: 474 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 523

BLAST of MS021268 vs. ExPASy TrEMBL
Match: A0A6J1CCM1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X6 OS=Momordica charantia OX=3673 GN=LOC111010433 PE=4 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.5e-245
Identity = 424/470 (90.21%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   KRTLAPSHTTPAIDLELDQQGQGTFSQRSNHSMVTAFRSSTNPEVQHQPEHANGAILYAM 60
           KRTLAPSHTT AIDLELDQQGQGTFSQRSNHSMVT FRSSTNPEVQHQPEHANGAILYAM
Sbjct: 40  KRTLAPSHTTSAIDLELDQQGQGTFSQRSNHSMVTGFRSSTNPEVQHQPEHANGAILYAM 99

Query: 61  SQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 120
           SQYSGSMIQRAQDM VATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV
Sbjct: 100 SQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 159

Query: 121 GSPYKRRFAEPVPVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 180
           GSPYKRRFAEPV VNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL
Sbjct: 160 GSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 219

Query: 181 WLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 240
           WLDQRFNCNFEDRGAWNWNHAPAGFP+HGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ
Sbjct: 220 WLDQRFNCNFEDRGAWNWNHAPAGFPVHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 279

Query: 241 HPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 300
           HPPSTLIQHPSYRIPLPQ HLQGQRGQNI LNPPVAAVTQRFPLSSAYGIMHQQFLELEP
Sbjct: 280 HPPSTLIQHPSYRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 339

Query: 301 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG------------------------- 360
           RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG                         
Sbjct: 340 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEGEAGVPGAQASASASDSNDIGDAYRD 399

Query: 361 -----------ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEET 420
                      ELLALEE+IGYVGTGLSEEIITSQLKTRISMPS RDANLEE ASRNEET
Sbjct: 400 MRLDIEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEET 459

Query: 421 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 435
           NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR
Sbjct: 460 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 509

BLAST of MS021268 vs. ExPASy TrEMBL
Match: A0A6J1CFW4 (probable E3 ubiquitin-protein ligase ZFP1 isoform X5 OS=Momordica charantia OX=3673 GN=LOC111010433 PE=4 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.5e-245
Identity = 424/470 (90.21%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   KRTLAPSHTTPAIDLELDQQGQGTFSQRSNHSMVTAFRSSTNPEVQHQPEHANGAILYAM 60
           KRTLAPSHTT AIDLELDQQGQGTFSQRSNHSMVT FRSSTNPEVQHQPEHANGAILYAM
Sbjct: 43  KRTLAPSHTTSAIDLELDQQGQGTFSQRSNHSMVTGFRSSTNPEVQHQPEHANGAILYAM 102

Query: 61  SQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 120
           SQYSGSMIQRAQDM VATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV
Sbjct: 103 SQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRSGLMGIPSDAYARNSHFIEEV 162

Query: 121 GSPYKRRFAEPVPVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 180
           GSPYKRRFAEPV VNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL
Sbjct: 163 GSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTRSHLARGEYMVQHLQPASNAL 222

Query: 181 WLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 240
           WLDQRFNCNFEDRGAWNWNHAPAGFP+HGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ
Sbjct: 223 WLDQRFNCNFEDRGAWNWNHAPAGFPVHGDNAIRGASENSNSGLHRYSEIGGMNCSPSFQ 282

Query: 241 HPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 300
           HPPSTLIQHPSYRIPLPQ HLQGQRGQNI LNPPVAAVTQRFPLSSAYGIMHQQFLELEP
Sbjct: 283 HPPSTLIQHPSYRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEP 342

Query: 301 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG------------------------- 360
           RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEG                         
Sbjct: 343 RQTRDLPFNDRRVYNPHETMFRRHLTSRMRVLQEGEAGVPGAQASASASDSNDIGDAYRD 402

Query: 361 -----------ELLALEERIGYVGTGLSEEIITSQLKTRISMPSGRDANLEEEASRNEET 420
                      ELLALEE+IGYVGTGLSEEIITSQLKTRISMPS RDANLEE ASRNEET
Sbjct: 403 MRLDIEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEET 462

Query: 421 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 435
           NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR
Sbjct: 463 NSCTICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 512

BLAST of MS021268 vs. TAIR 10
Match: AT1G53190.1 (RING/U-box superfamily protein )

HSP 1 Score: 149.1 bits (375), Expect = 8.5e-36
Identity = 141/505 (27.92%), Postives = 207/505 (40.99%), Query Frame = 0

Query: 13  IDLELDQQGQ------------GTFSQR---SNHSMVTAFRSSTNPEVQHQPEHANGAIL 72
           +DLE++QQ              G+F Q+   +N   + A   +  P     P + N A+ 
Sbjct: 8   VDLEMEQQQSQASLQAEPCILLGSFPQQPDNNNMPAMVAHVPNLEPHSLQDPTYDNSAMF 67

Query: 73  YAMSQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRS-GLMGI-PSDAYARNSH 132
           Y + QY     QR           P     +P G  Q PS  S G++G+ P   Y RN+H
Sbjct: 68  YGLPQYHHHPHQRVPTNFYV----PYVAFQAPPG--QLPSSSSHGVVGVSPDHEYERNAH 127

Query: 133 FIEEVGSPYKRRFAEPVPVNFQGPNSSA---SSESLNAPF-------------DPMFCHT 192
           F++     YKR+ AE +P   Q  ++ A   ++    APF               +  H 
Sbjct: 128 FMDHTRGTYKRKNAEGIPGQPQYLSTLAAPFNTPETIAPFGGARNRPGAVTVNTVLPSHA 187

Query: 193 RSHLARGEYMVQHLQPASNALWLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASEN 252
            ++  +G Y   H  P   ++W DQ    +      W     P  + +HG N   G+ E+
Sbjct: 188 PNNFIQGNYAGHHPFPPPGSMWYDQHHGRSDGSPSFW-----PTPY-MHGSNIFAGSIES 247

Query: 253 SNSGLHRYSEIGGMNCSPSFQHPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAA-- 312
           S+     +         PS  +P      H  +  P P   +QG RGQN +L P  A+  
Sbjct: 248 SSRNPTSF-------MYPSQLNPRDHYYSHHHHPAPPP---VQGMRGQNATLYPHTASSA 307

Query: 313 -------------VTQRFPLSSAYGIMHQQFLELEPRQTRDLPFNDRRVYNPHETMFRRH 372
                             PL S  G  H     ++P   R    + R    P  T+ R+H
Sbjct: 308 SYRVPPGSFTPQNTMNSGPLGSEMGSSHMG--PVQPTGFRIYQHHQRDDSVPVATL-RQH 367

Query: 373 L--TSRMRVLQEG-----------------------------------ELLALEERIGYV 432
                R+RV+ +                                    ELLAL ERIG V
Sbjct: 368 RGGVPRLRVMPDDEVALLEFGDFLGGSGNNHIDHHRDMRLDIEEMSYEELLALSERIGTV 427

BLAST of MS021268 vs. TAIR 10
Match: AT1G53190.2 (RING/U-box superfamily protein )

HSP 1 Score: 149.1 bits (375), Expect = 8.5e-36
Identity = 141/505 (27.92%), Postives = 207/505 (40.99%), Query Frame = 0

Query: 13  IDLELDQQGQ------------GTFSQR---SNHSMVTAFRSSTNPEVQHQPEHANGAIL 72
           +DLE++QQ              G+F Q+   +N   + A   +  P     P + N A+ 
Sbjct: 8   VDLEMEQQQSQASLQAEPCILLGSFPQQPDNNNMPAMVAHVPNLEPHSLQDPTYDNSAMF 67

Query: 73  YAMSQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFPSPRS-GLMGI-PSDAYARNSH 132
           Y + QY     QR           P     +P G  Q PS  S G++G+ P   Y RN+H
Sbjct: 68  YGLPQYHHHPHQRVPTNFYV----PYVAFQAPPG--QLPSSSSHGVVGVSPDHEYERNAH 127

Query: 133 FIEEVGSPYKRRFAEPVPVNFQGPNSSA---SSESLNAPF-------------DPMFCHT 192
           F++     YKR+ AE +P   Q  ++ A   ++    APF               +  H 
Sbjct: 128 FMDHTRGTYKRKNAEGIPGQPQYLSTLAAPFNTPETIAPFGGARNRPGAVTVNTVLPSHA 187

Query: 193 RSHLARGEYMVQHLQPASNALWLDQRFNCNFEDRGAWNWNHAPAGFPIHGDNAIRGASEN 252
            ++  +G Y   H  P   ++W DQ    +      W     P  + +HG N   G+ E+
Sbjct: 188 PNNFIQGNYAGHHPFPPPGSMWYDQHHGRSDGSPSFW-----PTPY-MHGSNIFAGSIES 247

Query: 253 SNSGLHRYSEIGGMNCSPSFQHPPSTLIQHPSYRIPLPQTHLQGQRGQNISLNPPVAA-- 312
           S+     +         PS  +P      H  +  P P   +QG RGQN +L P  A+  
Sbjct: 248 SSRNPTSF-------MYPSQLNPRDHYYSHHHHPAPPP---VQGMRGQNATLYPHTASSA 307

Query: 313 -------------VTQRFPLSSAYGIMHQQFLELEPRQTRDLPFNDRRVYNPHETMFRRH 372
                             PL S  G  H     ++P   R    + R    P  T+ R+H
Sbjct: 308 SYRVPPGSFTPQNTMNSGPLGSEMGSSHMG--PVQPTGFRIYQHHQRDDSVPVATL-RQH 367

Query: 373 L--TSRMRVLQEG-----------------------------------ELLALEERIGYV 432
                R+RV+ +                                    ELLAL ERIG V
Sbjct: 368 RGGVPRLRVMPDDEVALLEFGDFLGGSGNNHIDHHRDMRLDIEEMSYEELLALSERIGTV 427

BLAST of MS021268 vs. TAIR 10
Match: AT3G15070.1 (RING/U-box superfamily protein )

HSP 1 Score: 148.7 bits (374), Expect = 1.1e-35
Identity = 134/442 (30.32%), Postives = 196/442 (44.34%), Query Frame = 0

Query: 48  QPEHANGAILYAMSQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFP-------SPRS 107
           Q  + N ++ Y   QY     Q A ++    +  P  Y+  PY N + P           
Sbjct: 51  QEPYDNNSMFYGPPQYHH---QHASNLGSGMSTAPNFYV--PYVNYEAPPSFLLSHGSND 110

Query: 108 GLMGIPSDAYARNSHFIEEVGSPYKRRFAEPVPVNFQGPNSSASSESLN----APF---- 167
            ++G+ S  + RN+HF++     +KR+ +E +P N Q P +  S   LN    APF    
Sbjct: 111 AVVGVTSTEHERNAHFMDH---GFKRKSSEVIPGNSQYPVAPCSFPQLNTSETAPFSFPH 170

Query: 168 ----------------------DPMFCHTRSHLARGEYMVQHLQPASNALWLDQRFNCNF 227
                                 DP+  H  ++ ++G Y   H  P   ++W DQ  N N 
Sbjct: 171 FGTYPQPLDQRSVRNRAGAATMDPLLSHGHNNFSQGNY-AAHPFPPLGSIWYDQHCNGNR 230

Query: 228 EDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEI-GGMNCSPSFQHPPSTLIQH 287
            D  +  W+ APA   +HG N   G+ E+ N    RY E     N +PS       +  H
Sbjct: 231 SDGSSSLWSQAPAVPYMHG-NIATGSIESGNVCFPRYHETSSSRNPTPSVYQRNHYISHH 290

Query: 288 PSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQ----QFLELEPRQTRD 347
           P    P+   H+        +L+P  A+ +    + S    ++Q     F+     + R+
Sbjct: 291 PVPPPPIVYPHMPSASYAE-TLHP--ASYSHMGQVQSTGFRINQYPGEDFVPAAILRHRE 350

Query: 348 LPFNDRRVYNPHETMF-------------RRHLTSRMRV--LQEGELLALEERIGYVGTG 407
           LP    R    +E  F               H   R+ +  +   ELLAL ++IG V TG
Sbjct: 351 LP--HFRAMPANENAFWEVGDFYNAVNYVDHHQDMRLDIEDMSYEELLALSDQIGTVKTG 410

Query: 408 LSEEIITSQLKTRISMPSGRDANLEEEASRNEETNSCTICLDVIEDGKKIGILDCGHYYH 433
           LS E +   LK R S       NLEE  S + ET+SCTIC +  ++  KI  LDC H YH
Sbjct: 411 LSSEDVKELLKRRTS----TRINLEEGPSTDLETDSCTICQENYKNEDKIATLDCMHKYH 470

BLAST of MS021268 vs. TAIR 10
Match: AT3G15070.2 (RING/U-box superfamily protein )

HSP 1 Score: 148.7 bits (374), Expect = 1.1e-35
Identity = 134/442 (30.32%), Postives = 196/442 (44.34%), Query Frame = 0

Query: 48  QPEHANGAILYAMSQYSGSMIQRAQDMRVATAGNPCSYLTSPYGNEQFP-------SPRS 107
           Q  + N ++ Y   QY     Q A ++    +  P  Y+  PY N + P           
Sbjct: 51  QEPYDNNSMFYGPPQYHH---QHASNLGSGMSTAPNFYV--PYVNYEAPPSFLLSHGSND 110

Query: 108 GLMGIPSDAYARNSHFIEEVGSPYKRRFAEPVPVNFQGPNSSASSESLN----APF---- 167
            ++G+ S  + RN+HF++     +KR+ +E +P N Q P +  S   LN    APF    
Sbjct: 111 AVVGVTSTEHERNAHFMDH---GFKRKSSEVIPGNSQYPVAPCSFPQLNTSETAPFSFPH 170

Query: 168 ----------------------DPMFCHTRSHLARGEYMVQHLQPASNALWLDQRFNCNF 227
                                 DP+  H  ++ ++G Y   H  P   ++W DQ  N N 
Sbjct: 171 FGTYPQPLDQRSVRNRAGAATMDPLLSHGHNNFSQGNY-AAHPFPPLGSIWYDQHCNGNR 230

Query: 228 EDRGAWNWNHAPAGFPIHGDNAIRGASENSNSGLHRYSEI-GGMNCSPSFQHPPSTLIQH 287
            D  +  W+ APA   +HG N   G+ E+ N    RY E     N +PS       +  H
Sbjct: 231 SDGSSSLWSQAPAVPYMHG-NIATGSIESGNVCFPRYHETSSSRNPTPSVYQRNHYISHH 290

Query: 288 PSYRIPLPQTHLQGQRGQNISLNPPVAAVTQRFPLSSAYGIMHQ----QFLELEPRQTRD 347
           P    P+   H+        +L+P  A+ +    + S    ++Q     F+     + R+
Sbjct: 291 PVPPPPIVYPHMPSASYAE-TLHP--ASYSHMGQVQSTGFRINQYPGEDFVPAAILRHRE 350

Query: 348 LPFNDRRVYNPHETMF-------------RRHLTSRMRV--LQEGELLALEERIGYVGTG 407
           LP    R    +E  F               H   R+ +  +   ELLAL ++IG V TG
Sbjct: 351 LP--HFRAMPANENAFWEVGDFYNAVNYVDHHQDMRLDIEDMSYEELLALSDQIGTVKTG 410

Query: 408 LSEEIITSQLKTRISMPSGRDANLEEEASRNEETNSCTICLDVIEDGKKIGILDCGHYYH 433
           LS E +   LK R S       NLEE  S + ET+SCTIC +  ++  KI  LDC H YH
Sbjct: 411 LSSEDVKELLKRRTS----TRINLEEGPSTDLETDSCTICQENYKNEDKIATLDCMHKYH 470

BLAST of MS021268 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 8.5e-20
Identity = 53/136 (38.97%), Postives = 71/136 (52.21%), Query Frame = 0

Query: 299 EPRQTRDLPFNDRRVYNPHETMFRRHLTSRMRV--LQEGELLALEERIGYVGTGLSEEII 358
           E  +  D    D  +Y     M  RH   R+ V  +   ELLAL ERIG V TGLSEE+I
Sbjct: 571 ENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELLALGERIGDVSTGLSEEVI 630

Query: 359 TSQLKTRISMPSGRDANLEEEASRNEETNSCTICLDVIEDGKKIGILDCGHYYHADCLKQ 418
              +K      S            ++    C IC +   +G  +G L CGH +H DC+KQ
Sbjct: 631 LKAMKQHKHTSSS-----PSSVELHQNIEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQ 690

Query: 419 WLLIKNVCPVCKSEAL 433
           W++IKN+CP+CK+EAL
Sbjct: 691 WVMIKNLCPICKTEAL 701