MS021147 (gene) Bitter gourd (TR) v1

Overview
NameMS021147
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontricalbin-3-like
Locationscaffold989: 59640 .. 64435 (-)
RNA-Seq ExpressionMS021147
SyntenyMS021147
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTATGCACTTTACATCCGCTAGCTTTGGCTTCTCGAACTCCTTTTCTCAACATTGCCCATGTAATAACTTCACTGTCTTCACCCCCTCTCACACAACTCCCAACCCCCTTTCCACTCCTTTATCTAGAAAAAGAAGAAAACAGTTTTCCGCCGCTTTCGTTCACCGATATTTCAGAAGAAAATGGTTCTTGGCTTGTTCTATTTCTCCCGATGGCGCAAGTCGCAACTTGAATCTAGATTTTGCAAGTTCCGCGAGGAGGGGTGCCAAAAGTTTTGTAGTGAAGCATATTGCCGATGAATTGGAAAGGGAAGAATTCTCCCAGGACTCATCTGTGCAAGTGGGATCAAATTTCACTGGTTTCCAGGAGGATCCTATTGTTGATAAGTTACGGACCCAGCTAGGAGTCATACACCCCATCCCTTCGCCACCTATTAATCGAAACATTGTTGGACTTTTTGTCTTCTTTTTCTTTGTTGGAGTTGTCTTCGACAAGTTATGGACATTTAGAAGGAGAAATAAATCGAGAAATGAGGACGGGCGTCGCGGCACATGGCCGCAGGTGCCGACTAGTTTCTCGTCGTTTTTGGAGAAGGATTTACAAAGGAAAGAGTCGGTGGAGTGGGTGAATATGGTTTTGGGTAAGTTATGGAAAGTCTATCGACCTGGTCTCGAGAATTGGCTTGTCGGGTTGTTGCAACCCGTTATTGATAATTTGAAAAAACCTGATTATGTGGAGAGGGTGGAAATTAAGCAGTTCTCCTTGGGGGAAGAACCACTGTCAGTTAGGAATGTTGAGAGGCGAACATCCCGTCGAGCCAACGATTTGCAGTAAGTTAATTTTAAATCCGTCTTTCAAACTTCACATGTACCTTTTGGCTGTGGATTCAATCCCTTCCTGCGATGTTTACTGGCGATACCTTTCCATGAATTTACACTAGTCATTATTGTTTTTCAATGCTGTCCAGGTACCAAATTGGTCTGCGTTATACTGGCGGTGCTCGTATGTTATTAATGCTATCGCTAAAATTTGGCATCATCCCCATTGTTGTTCCTGTTGGTGTCCGAGATTTTGACATAGATGGTGAATTATGGGTCAAGTTGCGGCTTATTCCAGCTGAGCCTTGGGTAGGTGCTGTCTCATGGGCATTTGTTTCACTCCCAAAGATCAAATTTGAGTTGTCACCGTTCCGCTTGTTCAATTTAATGGGTATGTTATGTCGTACTTTCTTGACTTTGATAACGAATTACATTTTATAGCTAGTCACTGCAAGGACGCCATAGTGAGGTTTTACAGGGAAGCATGAAAGGTGTTTTAATTTTATGTTCTTTAGTTTATCCTTATGTTTTTAGTAGGCATGGTTGTGGAGGTAAAATTTGTTTCTTAAGAATAGTTTTCTTTGCTCTAAATTTGACGTTTAGATGCTGATTGTACGTTTCTCTCTTTTTTCCATCTTTTTGCTGCACGCTTGGGAAAACATCAGCCATTCCTGTTCTCTCCATGTAAGTACAACTTTAATCTTAATTGCTTATATAGTTGCTTTATAAAGTTGCTTAGCTCTAGGAAGGAATAGAGGCAATCCTTTCCATATTAGCATTGCTGATTGCTCTTTCTCTCTGAGAACTTTTGTTTCTTTTCTTTTTGAGTTCAACAAGTGGGAGTGAGAATTCAAATCTTCGACCTTTTGGTCAAGGATATATGCTTTAACCAGTTGTTCTAGGCTTATTGGCCTCTATGATAAACTTTTGTCTATATCACTATTTTGACTATATTTTGCCAGAAAGTGGTGCATTGATATTCTATATCACATTTGTACCATTGAATATTTTTCTAGTTCAATTCATGATTTGTACGAGCTAATGATAGCCTGAATTCCTTGGCAATAGTTCTCAGACTAGAAAAATAAGACTGCTATTATTGAAGCTGGATCGTGTTATGAAGTAGTGCCCAACTATACTAATAACGAATTAAATATATTGATCAGAATCAGTTTTCTGAGTGTCATCATTGTGCAGTGGCAGTTCACAGCATAATTTGCTCATGTAGCTGATAATTATATCCAAGTATTCTAGTTATTTTTAGGCTTGCTTTTCCCAATCCAGCTGACCTGAATGCTTATTATGTTTCGTAGGTTTTTGACAAAACTTCTCACTGAGGATTTACCTAAATTGTTTGTACGGCCAAAAAAGATAGTTCTAGATTTCCAAAAAGGTAAAGCAGTTGGACCTGTTACAGACGAGGTTAAATCAGGAGACATGCAGGAGGGAAATAAAGATTCCGTTGGGGAACTTTCAATAACCCTTGTAGATGCTCGGAAGCTTTCTTACGTCTTCTATGGTAATAATTGCTATTTTATCCATTTTTTCCATTCTTGGAAACCATGCTTTGTGAATTACTGAATTTCTGCACTCAACTGTCAAGTATTTGTGGTGCCTATCAACATCAACATGATACTGACACTTGCAAAAACAATTTTTTTGTAGAACCTAATGTATTCACATATCTCCATTAGAAGTTCCTAAATGGAACTACTTTATGTCTTTGATAGAACATATTTTTTAATATAAATATAATTTTCTTTTTATTTTTATAAAATATATTCTCTAATGTATCTCTGTATCGTTTACCCCTACAAAATACCAAAAAAAAATTGAACCATCTTAGAAGGGATATAATTAAATTCCTTGATTGGTTCTTCCCTGAGAAATGATCCCTGCTTTCAGAAAGTTACCATCTGATTTCACAGTCAGATAGTAATTACTATAAATTTGTGCTTCTTTATTTTACTCATTGATCTGATTCCTGTTATTCTCAGGAAAAACAGACCCATACGTCATTTTAAGCTTGGGTGATCAAATAATACGCAGCAAAAAGAACAGTCAGACCACTGTCATTGGACCACCTGGTGAGCCAATCTGGAATCAGGTGGAGCTCATTTCTGATTATCTTTTGTTTTACCGGAGAACATGTTCCATTAACAATATTGGTCAAACTGTTATTTGGTTGTTTTTGGGTTCATTCGAAGTTGTCAATCTCTTTATGTACTCACGTGTATTGGGGACATTGGCAGGATTTTCATATGCTTGTTGCAAATCCTAGGAAACAGAAATTGTACATCCAAGTTAAAGATTCTCTTGGATTTGCAGATTTGACCGTTGGTAATGCAGAGGTAACTTATTATTTAAACTTATGATAGTTAGACTTCGAAATATCTAATGATATTGCATTTAAGGAGAAGTTGAAACTGGTGAAACCATGCTTCATTTCTTTGTTTGCTTATCTTTATATTCGATCAGTAACTGTCCATCGTATCTTTATCTCTCTTGTAACTGTGCAAACTTAATCGAGGAAACTAAAGCTGCTGAACTGACTATGTTGGGTTAGGTTTTTGGTCTATTCTAACGATAAAAATCAGTTTGGTCATTTTCAAGGTTGGTTTAGTTTTACTTTTAGTCCCAAAAATTGACTGACTATTACTCTAATTTTAGTTTGTTTTTTCTATAATTTGGGGGTTGGATGTCTTTAAATCATTGGATCATTTATTTGTTTGAAGTTCTAACTAGATTTTCTTACTCTGCACTTGGATGGGACCTCTACATGCCTTGAGCATCTTGTATAACCAGCATTCTTGGTGAGAAGTGTTTGCTGATGCAAACTATCTATAGAACAAGTTGTATAAAATGACGGTAATGCTAGTAGATAGCATTGGATAGTAATAGTATAATTGTTTTGCGTGGTTTTGGGAGTCTTGACAAGGGGATCACATCATCCTGAAAGAAATTAGGAAGTGAAATAAAACGTTGGATGTTGCTAAAGAGAAAGCAGATGTTTGATCTCATTAAATCATCTTCAAGGTGGCAGAAGTCACGCCTCCTATTACATCTTGAAGCACGGATTTATGACCACAATAACAATTAGATCATCCATTGGGTACTTAAATGGTTCTAGAATAGAATGATTCTGGCTTGTGCCAGAGGCAAAGTCTGTGTTCTGCATTTGGAACCGATTGAAATGATAAAGCACTTGTGTAATGAGAACAATGTATGGATTGGTAAATGAATGGTGCAAATTAATTTTTTTTTTATGGCTGTGTTTTTGTGTATGGTAACATTCTTCACAAAAAACATACAGGTCGATCTTGGATCTCTTCAAGATACTGTACCAACAGACAAAATTGTTGTTTTAGGAGGAGGTTGGGGACTCTTTAGAAATAGGTCTTCTGGAGAAATACTACTGAGATTGACATACAAAGCATATGTTGAGGATGAAGAGGACGACAAGGCTGCTGCATCAGAAGCAAATGTGTTGGATATAGATATTTCAGATGACGAGTCATCTGATTCTGATGAACCAAATGGTGCTTATGAGAAAGAGGGTGAGAAGGATGCTGCAAAAGAAACTGAAAAGGAATCGTTCATGGACGTTCTAGCGGCTTTGATAGTGAGCGAAGAATTTCAGGGGATAGTGGCATCCGAAACACTGAATACAATACTTCAGAATGATGCCACTTCTACTAGCACAAGGACGACAAGGTCGAGCTCAAGATCACGTGATGCGGCTACTGACACCAAAACCAAACCCATGGTTTCTGGCAACGGGACTGGAGGTTTAACTGGTAATGTTGTTGTCTAGTTTCGTATATATCTGTATTTTAATGTGATGTTTGGATGGTGGTGAAGTGTTCTAATATGACGTGGACAATTGCAGAATCGGCATTGTTTTGGCTTGCTGTGATCACAAGTATATCTGTGCTTATTGCTATCAACATTGGTGGTTCGAATTTCTTCAATCCA

mRNA sequence

ATGATTATGCACTTTACATCCGCTAGCTTTGGCTTCTCGAACTCCTTTTCTCAACATTGCCCATGTAATAACTTCACTGTCTTCACCCCCTCTCACACAACTCCCAACCCCCTTTCCACTCCTTTATCTAGAAAAAGAAGAAAACAGTTTTCCGCCGCTTTCGTTCACCGATATTTCAGAAGAAAATGGTTCTTGGCTTGTTCTATTTCTCCCGATGGCGCAAGTCGCAACTTGAATCTAGATTTTGCAAGTTCCGCGAGGAGGGGTGCCAAAAGTTTTGTAGTGAAGCATATTGCCGATGAATTGGAAAGGGAAGAATTCTCCCAGGACTCATCTGTGCAAGTGGGATCAAATTTCACTGGTTTCCAGGAGGATCCTATTGTTGATAAGTTACGGACCCAGCTAGGAGTCATACACCCCATCCCTTCGCCACCTATTAATCGAAACATTGTTGGACTTTTTGTCTTCTTTTTCTTTGTTGGAGTTGTCTTCGACAAGTTATGGACATTTAGAAGGAGAAATAAATCGAGAAATGAGGACGGGCGTCGCGGCACATGGCCGCAGGTGCCGACTAGTTTCTCGTCGTTTTTGGAGAAGGATTTACAAAGGAAAGAGTCGGTGGAGTGGGTGAATATGGTTTTGGGTAAGTTATGGAAAGTCTATCGACCTGGTCTCGAGAATTGGCTTGTCGGGTTGTTGCAACCCGTTATTGATAATTTGAAAAAACCTGATTATGTGGAGAGGGTGGAAATTAAGCAGTTCTCCTTGGGGGAAGAACCACTGTCAGTTAGGAATGTTGAGAGGCGAACATCCCGTCGAGCCAACGATTTGCAGTACCAAATTGGTCTGCGTTATACTGGCGGTGCTCGTATGTTATTAATGCTATCGCTAAAATTTGGCATCATCCCCATTGTTGTTCCTGTTGGTGTCCGAGATTTTGACATAGATGGTGAATTATGGGTCAAGTTGCGGCTTATTCCAGCTGAGCCTTGGGTAGGTGCTGTCTCATGGGCATTTGTTTCACTCCCAAAGATCAAATTTGAGTTGTCACCGTTCCGCTTGTTCAATTTAATGGGTATGTTTTTGACAAAACTTCTCACTGAGGATTTACCTAAATTGTTTGTACGGCCAAAAAAGATAGTTCTAGATTTCCAAAAAGGTAAAGCAGTTGGACCTGTTACAGACGAGGTTAAATCAGGAGACATGCAGGAGGGAAATAAAGATTCCGTTGGGGAACTTTCAATAACCCTTGTAGATGCTCGGAAGCTTTCTTACGTCTTCTATGGAAAAACAGACCCATACGTCATTTTAAGCTTGGGTGATCAAATAATACGCAGCAAAAAGAACAGTCAGACCACTGTCATTGGACCACCTGGTGAGCCAATCTGGAATCAGGATTTTCATATGCTTGTTGCAAATCCTAGGAAACAGAAATTGTACATCCAAGTTAAAGATTCTCTTGGATTTGCAGATTTGACCGTTGGTAATGCAGAGGTCGATCTTGGATCTCTTCAAGATACTGTACCAACAGACAAAATTGTTGTTTTAGGAGGAGGTTGGGGACTCTTTAGAAATAGGTCTTCTGGAGAAATACTACTGAGATTGACATACAAAGCATATGTTGAGGATGAAGAGGACGACAAGGCTGCTGCATCAGAAGCAAATGTGTTGGATATAGATATTTCAGATGACGAGTCATCTGATTCTGATGAACCAAATGGTGCTTATGAGAAAGAGGGTGAGAAGGATGCTGCAAAAGAAACTGAAAAGGAATCGTTCATGGACGTTCTAGCGGCTTTGATAGTGAGCGAAGAATTTCAGGGGATAGTGGCATCCGAAACACTGAATACAATACTTCAGAATGATGCCACTTCTACTAGCACAAGGACGACAAGGTCGAGCTCAAGATCACGTGATGCGGCTACTGACACCAAAACCAAACCCATGGTTTCTGGCAACGGGACTGGAGGTTTAACTGAATCGGCATTGTTTTGGCTTGCTGTGATCACAAGTATATCTGTGCTTATTGCTATCAACATTGGTGGTTCGAATTTCTTCAATCCA

Coding sequence (CDS)

ATGATTATGCACTTTACATCCGCTAGCTTTGGCTTCTCGAACTCCTTTTCTCAACATTGCCCATGTAATAACTTCACTGTCTTCACCCCCTCTCACACAACTCCCAACCCCCTTTCCACTCCTTTATCTAGAAAAAGAAGAAAACAGTTTTCCGCCGCTTTCGTTCACCGATATTTCAGAAGAAAATGGTTCTTGGCTTGTTCTATTTCTCCCGATGGCGCAAGTCGCAACTTGAATCTAGATTTTGCAAGTTCCGCGAGGAGGGGTGCCAAAAGTTTTGTAGTGAAGCATATTGCCGATGAATTGGAAAGGGAAGAATTCTCCCAGGACTCATCTGTGCAAGTGGGATCAAATTTCACTGGTTTCCAGGAGGATCCTATTGTTGATAAGTTACGGACCCAGCTAGGAGTCATACACCCCATCCCTTCGCCACCTATTAATCGAAACATTGTTGGACTTTTTGTCTTCTTTTTCTTTGTTGGAGTTGTCTTCGACAAGTTATGGACATTTAGAAGGAGAAATAAATCGAGAAATGAGGACGGGCGTCGCGGCACATGGCCGCAGGTGCCGACTAGTTTCTCGTCGTTTTTGGAGAAGGATTTACAAAGGAAAGAGTCGGTGGAGTGGGTGAATATGGTTTTGGGTAAGTTATGGAAAGTCTATCGACCTGGTCTCGAGAATTGGCTTGTCGGGTTGTTGCAACCCGTTATTGATAATTTGAAAAAACCTGATTATGTGGAGAGGGTGGAAATTAAGCAGTTCTCCTTGGGGGAAGAACCACTGTCAGTTAGGAATGTTGAGAGGCGAACATCCCGTCGAGCCAACGATTTGCAGTACCAAATTGGTCTGCGTTATACTGGCGGTGCTCGTATGTTATTAATGCTATCGCTAAAATTTGGCATCATCCCCATTGTTGTTCCTGTTGGTGTCCGAGATTTTGACATAGATGGTGAATTATGGGTCAAGTTGCGGCTTATTCCAGCTGAGCCTTGGGTAGGTGCTGTCTCATGGGCATTTGTTTCACTCCCAAAGATCAAATTTGAGTTGTCACCGTTCCGCTTGTTCAATTTAATGGGTATGTTTTTGACAAAACTTCTCACTGAGGATTTACCTAAATTGTTTGTACGGCCAAAAAAGATAGTTCTAGATTTCCAAAAAGGTAAAGCAGTTGGACCTGTTACAGACGAGGTTAAATCAGGAGACATGCAGGAGGGAAATAAAGATTCCGTTGGGGAACTTTCAATAACCCTTGTAGATGCTCGGAAGCTTTCTTACGTCTTCTATGGAAAAACAGACCCATACGTCATTTTAAGCTTGGGTGATCAAATAATACGCAGCAAAAAGAACAGTCAGACCACTGTCATTGGACCACCTGGTGAGCCAATCTGGAATCAGGATTTTCATATGCTTGTTGCAAATCCTAGGAAACAGAAATTGTACATCCAAGTTAAAGATTCTCTTGGATTTGCAGATTTGACCGTTGGTAATGCAGAGGTCGATCTTGGATCTCTTCAAGATACTGTACCAACAGACAAAATTGTTGTTTTAGGAGGAGGTTGGGGACTCTTTAGAAATAGGTCTTCTGGAGAAATACTACTGAGATTGACATACAAAGCATATGTTGAGGATGAAGAGGACGACAAGGCTGCTGCATCAGAAGCAAATGTGTTGGATATAGATATTTCAGATGACGAGTCATCTGATTCTGATGAACCAAATGGTGCTTATGAGAAAGAGGGTGAGAAGGATGCTGCAAAAGAAACTGAAAAGGAATCGTTCATGGACGTTCTAGCGGCTTTGATAGTGAGCGAAGAATTTCAGGGGATAGTGGCATCCGAAACACTGAATACAATACTTCAGAATGATGCCACTTCTACTAGCACAAGGACGACAAGGTCGAGCTCAAGATCACGTGATGCGGCTACTGACACCAAAACCAAACCCATGGTTTCTGGCAACGGGACTGGAGGTTTAACTGAATCGGCATTGTTTTGGCTTGCTGTGATCACAAGTATATCTGTGCTTATTGCTATCAACATTGGTGGTTCGAATTTCTTCAATCCA

Protein sequence

MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFRRKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQDSSVQVGSNFTGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNEDGRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNLKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFGIIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLMGMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSITLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPRKQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRLTYKAYVEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGEKDAAKETEKESFMDVLAALIVSEEFQGIVASETLNTILQNDATSTSTRTTRSSSRSRDAATDTKTKPMVSGNGTGGLTESALFWLAVITSISVLIAINIGGSNFFNP
Homology
BLAST of MS021147 vs. NCBI nr
Match: XP_022151857.1 (tricalbin-3-like [Momordica charantia])

HSP 1 Score: 1335.5 bits (3455), Expect = 0.0e+00
Identity = 684/693 (98.70%), Postives = 684/693 (98.70%), Query Frame = 0

Query: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60
           MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR
Sbjct: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60

Query: 61  RKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQDSSVQVGSNFT 120
           RKWFLACSISPDGASRNLNLDFASS RRGAKSFVVKHIADELEREEFSQDSSVQVGSNFT
Sbjct: 61  RKWFLACSISPDGASRNLNLDFASSTRRGAKSFVVKHIADELEREEFSQDSSVQVGSNFT 120

Query: 121 GFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNED 180
           GFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNED
Sbjct: 121 GFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNED 180

Query: 181 GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNL 240
           GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNL
Sbjct: 181 GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNL 240

Query: 241 KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 300
           KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG
Sbjct: 241 KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 300

Query: 301 IIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM-- 360
           IIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM  
Sbjct: 301 IIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLMAI 360

Query: 361 ---GMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSI 420
               MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSI
Sbjct: 361 PVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSI 420

Query: 421 TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPR 480
           TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPR
Sbjct: 421 TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPR 480

Query: 481 KQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRL 540
           KQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRL
Sbjct: 481 KQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRL 540

Query: 541 TYKAYVEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGEKDAAKETEKESFMD 600
           TYKAYVEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGEKDAAKETEKESFMD
Sbjct: 541 TYKAYVEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGEKDAAKETEKESFMD 600

Query: 601 VLAALIVSEEFQGIVASETLNTILQNDATSTSTRTTRSSSRSRDAATDTKTKPMVSGNGT 660
           VLAALIVSEEFQGIV SETLNTILQNDATSTSTRTTRSSSRSRDAATDTKTKPMVSGNGT
Sbjct: 601 VLAALIVSEEFQGIVTSETLNTILQNDATSTSTRTTRSSSRSRDAATDTKTKPMVSGNGT 660

Query: 661 GGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           GGLTESALFWLA ITSISVLIAINIGGSNFFNP
Sbjct: 661 GGLTESALFWLAAITSISVLIAINIGGSNFFNP 693

BLAST of MS021147 vs. NCBI nr
Match: XP_038892158.1 (tricalbin-3-like [Benincasa hispida])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 597/696 (85.78%), Postives = 630/696 (90.52%), Query Frame = 0

Query: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60
           MI HF   SF FSNSFSQHCPCNN T F+PSH   N LS    RK RK FSAA     FR
Sbjct: 1   MITHFPFPSFDFSNSFSQHCPCNNSTAFSPSHPPRNSLSPTSLRKSRKPFSAAL---NFR 60

Query: 61  RKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQD--SSVQVGSN 120
           RKWFL CS+SPDG +RN NL+FA+SARRGA++FVVK I++ELE EEFSQ+  SSVQVGSN
Sbjct: 61  RKWFLVCSLSPDGVTRNFNLEFATSARRGARNFVVKRISNELEGEEFSQEESSSVQVGSN 120

Query: 121 FTGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRN 180
           FTGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGV FDKLWTFR+R+KSRN
Sbjct: 121 FTGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVAFDKLWTFRKRSKSRN 180

Query: 181 EDGRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVID 240
           +DGRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPG+E+WLVGLLQPVID
Sbjct: 181 DDGRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGIEDWLVGLLQPVID 240

Query: 241 NLKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLK 300
           NLKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLK
Sbjct: 241 NLKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLK 300

Query: 301 FGIIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM 360
           FGIIPI VPVGVRDFDIDGELWVKLRLIP EPWVGAVSWAFVSLPKIKFELSPFRLFNLM
Sbjct: 301 FGIIPISVPVGVRDFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKFELSPFRLFNLM 360

Query: 361 -----GMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGEL 420
                 MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPV DEVKSGDMQEGN DSVGEL
Sbjct: 361 AIPVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVPDEVKSGDMQEGNNDSVGEL 420

Query: 421 SITLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVAN 480
           S+TLVDARKLSY+FYGKTDPYVILSLGDQ+IRSKKNSQTTVIGPPGEPIWNQDFHMLVAN
Sbjct: 421 SVTLVDARKLSYLFYGKTDPYVILSLGDQVIRSKKNSQTTVIGPPGEPIWNQDFHMLVAN 480

Query: 481 PRKQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILL 540
           PRKQKLYIQVKDSLGFADLT+GNAEVD+GSLQDTVPTD+IVVLGGGWGLFRNRSSGE+L+
Sbjct: 481 PRKQKLYIQVKDSLGFADLTIGNAEVDIGSLQDTVPTDRIVVLGGGWGLFRNRSSGEVLV 540

Query: 541 RLTYKAYVEDEEDDKAAASEANVLDIDIS-DDESSDSDEPNGAYEKEGEKDAAKETEKES 600
           RLTYKAYVEDEEDDKAA   ++ LDIDIS DDESSDSDEPNGAYE E E DAAK T+KE+
Sbjct: 541 RLTYKAYVEDEEDDKAA---SDALDIDISDDDESSDSDEPNGAYE-ESENDAAKTTDKET 600

Query: 601 FMDVLAALIVSEEFQGIVASETLNTILQNDATSTSTRTTRSSSRSRDAATDTKTKPMVSG 660
           FMDVLAALIVSEEFQGIVAS+ LNT +QNDAT + +  T   SRSRDAA D K+  MVS 
Sbjct: 601 FMDVLAALIVSEEFQGIVASDALNTKIQNDATISPSLGT-MKSRSRDAAADNKS--MVSS 660

Query: 661 NGTGGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
            G GGL ESALFWLAVITSISVLIAINIGGS+FFNP
Sbjct: 661 KGAGGLAESALFWLAVITSISVLIAINIGGSSFFNP 686

BLAST of MS021147 vs. NCBI nr
Match: XP_008445968.1 (PREDICTED: tricalbin-3-like [Cucumis melo] >TYK15771.1 tricalbin-3-like [Cucumis melo var. makuwa])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 586/696 (84.20%), Postives = 617/696 (88.65%), Query Frame = 0

Query: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60
           MI HF   +F FSNSFSQHCPCNNFT F            P SRK RK FS++     FR
Sbjct: 1   MITHFPFPTFDFSNSFSQHCPCNNFTAF-----------VPFSRKSRKPFSSSL---NFR 60

Query: 61  RKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQ-DSSVQVGSNF 120
           R+WFL CS+SPDG + N NL+FA+SARRG ++FVVK I++ELE EEFSQ +SSVQVGSNF
Sbjct: 61  RRWFLVCSLSPDGVTSNFNLEFATSARRGVRNFVVKRISNELEGEEFSQEESSVQVGSNF 120

Query: 121 TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNE 180
           TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGV FDKLWTFR+R+KSRN+
Sbjct: 121 TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVAFDKLWTFRKRSKSRND 180

Query: 181 DGRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDN 240
           DGR GTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPG+E+WLVGLLQPVIDN
Sbjct: 181 DGRLGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGIEDWLVGLLQPVIDN 240

Query: 241 LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF 300
           LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF
Sbjct: 241 LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF 300

Query: 301 GIIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM- 360
           GIIPIVVPV VRDFDIDGELWVKLRLIP EPWVGAVSWAFVSLPKIKFELSPFRLFNLM 
Sbjct: 301 GIIPIVVPVVVRDFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKFELSPFRLFNLMA 360

Query: 361 ----GMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELS 420
                MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPV DEVKSG MQEGN D VGELS
Sbjct: 361 IPVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVPDEVKSGVMQEGNNDFVGELS 420

Query: 421 ITLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANP 480
           +TLVDARKLSY+FYGKTDPYV+LSLGDQ IRSKKNSQTTVIGPPGEPIWNQDFHMLVANP
Sbjct: 421 VTLVDARKLSYLFYGKTDPYVVLSLGDQTIRSKKNSQTTVIGPPGEPIWNQDFHMLVANP 480

Query: 481 RKQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLR 540
           RKQKLYIQVKDSLGFADLT+GNAEVDLGSLQDTVPTD IVVLGGGWG FRNRSSGE+L+R
Sbjct: 481 RKQKLYIQVKDSLGFADLTIGNAEVDLGSLQDTVPTDTIVVLGGGWGFFRNRSSGEVLVR 540

Query: 541 LTYKAYVEDEEDDKAAASEANVLDIDIS-DDESSDSDEPNGAYEKEGEKDAAKETEKESF 600
           LTYKAYVEDEEDDKAA   +N LDIDIS DDESSD+DEPNGAYE E E D  K T KESF
Sbjct: 541 LTYKAYVEDEEDDKAA---SNALDIDISDDDESSDTDEPNGAYE-ESENDTVKATGKESF 600

Query: 601 MDVLAALIVSEEFQGIVASETLNTILQNDAT-STSTRTTRSSSRSRDAATDTKTKPMVSG 660
           MDVLAALIVSEEF GIVAS+ LNT LQNDAT STS+ TT  +SRSRD ATD   KP VS 
Sbjct: 601 MDVLAALIVSEEFLGIVASDALNTKLQNDATLSTSSGTT--NSRSRDTATD--NKPTVSS 660

Query: 661 NGTGGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           NG GGL +SALFWL VITSISVLIAINIGGS+FFNP
Sbjct: 661 NGAGGLADSALFWLTVITSISVLIAINIGGSSFFNP 674

BLAST of MS021147 vs. NCBI nr
Match: XP_004135352.1 (tricalbin-3 [Cucumis sativus] >KGN51626.1 hypothetical protein Csa_008247 [Cucumis sativus])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 578/696 (83.05%), Postives = 610/696 (87.64%), Query Frame = 0

Query: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60
           MI HF   +F FSNSFSQHCPCNNFT F            P SRK RK FS+      FR
Sbjct: 1   MITHFPFPTFDFSNSFSQHCPCNNFTAF-----------VPFSRKSRKPFSSTLT---FR 60

Query: 61  RKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQ-DSSVQVGSNF 120
           R+WFL CS+SPDG + N +L+FA+SARRG ++FVV  I++ELE EEFSQ +SSVQVGSNF
Sbjct: 61  RRWFLVCSLSPDGVTSNFDLEFATSARRGVRNFVVNRISNELEGEEFSQEESSVQVGSNF 120

Query: 121 TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNE 180
           TGFQEDPIVDKLRTQLG IHPIPSPPINRNIVGLFVFFFFVGV FDKLWTFR+R+KSRN 
Sbjct: 121 TGFQEDPIVDKLRTQLGAIHPIPSPPINRNIVGLFVFFFFVGVAFDKLWTFRKRSKSRNN 180

Query: 181 DGRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDN 240
           DGR GTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPG+E+WLVGLLQPVIDN
Sbjct: 181 DGRLGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGIEDWLVGLLQPVIDN 240

Query: 241 LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF 300
           LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF
Sbjct: 241 LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF 300

Query: 301 GIIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM- 360
           GIIPIVVPV VRDFDIDGELWVKLRLIP EPWVGAVSWAFVSLPKIKFELSPFRLFNLM 
Sbjct: 301 GIIPIVVPVVVRDFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKFELSPFRLFNLMA 360

Query: 361 ----GMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELS 420
                MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPV DEVKSG MQEGN D VGELS
Sbjct: 361 IPVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVPDEVKSGVMQEGNNDFVGELS 420

Query: 421 ITLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANP 480
           +TLVDARKLSY+FYGKTDPYV+LSLGDQ IRSKKNSQTTVIGPPGEPIWNQDFHMLVANP
Sbjct: 421 VTLVDARKLSYLFYGKTDPYVVLSLGDQTIRSKKNSQTTVIGPPGEPIWNQDFHMLVANP 480

Query: 481 RKQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLR 540
           RKQKLYIQVKDSLGFADLT+GNAEVDLGSLQDTVPTD IVVL  GWGLFRNRSSGE+L+R
Sbjct: 481 RKQKLYIQVKDSLGFADLTIGNAEVDLGSLQDTVPTDTIVVLREGWGLFRNRSSGEVLVR 540

Query: 541 LTYKAYVEDEEDDKAAASEANVLDIDIS-DDESSDSDEPNGAYEKEGEKDAAKETEKESF 600
           LTYKAYVEDEEDDKAA   ++ LDIDIS DDESSD+DEPNG YE E E D  K T KESF
Sbjct: 541 LTYKAYVEDEEDDKAA---SDALDIDISDDDESSDTDEPNGVYE-ESENDGVKATGKESF 600

Query: 601 MDVLAALIVSEEFQGIVASETLNTILQNDAT-STSTRTTRSSSRSRDAATDTKTKPMVSG 660
           MDVLAALIVSEEF GIVAS+ LNT LQND T STS+ TT  +SRSRD A D   KP VS 
Sbjct: 601 MDVLAALIVSEEFLGIVASDALNTKLQNDPTISTSSGTT--NSRSRDTAID--NKPTVSS 660

Query: 661 NGTGGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           NG+GGL +SALFWL VITSISVLIAINIGGS+FFNP
Sbjct: 661 NGSGGLADSALFWLTVITSISVLIAINIGGSSFFNP 674

BLAST of MS021147 vs. NCBI nr
Match: KAG6601632.1 (Extended synaptotagmin-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 580/697 (83.21%), Postives = 607/697 (87.09%), Query Frame = 0

Query: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60
           MI H TS  F  SNSF QH P +NF                ++  R    +AAF+ R  R
Sbjct: 1   MITHSTSLPFDSSNSFPQHSPSHNF----------------ITGARNSLSTAAFLPRNPR 60

Query: 61  RKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQDSSVQVGSNFT 120
           RK F   S+SPD A+R+ NL FA+ ARR AK FVVK   +ELEREEFSQDSSVQVGS+FT
Sbjct: 61  RKRFFPSSLSPDAATRHFNLQFATLARRSAKFFVVKRTTNELEREEFSQDSSVQVGSSFT 120

Query: 121 GFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNED 180
            FQEDPIVDKLRTQLGVIHPIPSPPINRNIV LFVFFFFVGV FDKLWT R+R+KSRNED
Sbjct: 121 AFQEDPIVDKLRTQLGVIHPIPSPPINRNIVALFVFFFFVGVAFDKLWTLRKRSKSRNED 180

Query: 181 GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNL 240
           GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLE+WLVGLLQPVIDNL
Sbjct: 181 GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLEDWLVGLLQPVIDNL 240

Query: 241 KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 300
           KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG
Sbjct: 241 KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 300

Query: 301 IIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM-- 360
           IIPI VPVGVR+FDIDGELWVKLRLIP EPWVGAVSWAFVSLPKIKFELSPFRLFNLM  
Sbjct: 301 IIPITVPVGVRNFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKFELSPFRLFNLMAI 360

Query: 361 ---GMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSI 420
               MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPV DEVKSGD+QEGNK  VGELS+
Sbjct: 361 PVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVPDEVKSGDIQEGNKGFVGELSV 420

Query: 421 TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPR 480
           TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIG PGEPIWNQDFHMLVANPR
Sbjct: 421 TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGSPGEPIWNQDFHMLVANPR 480

Query: 481 KQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRL 540
           KQKLYIQVKDSLGFADLT+GNAEVDLGSLQDTVPTD+IVVLGGGWGLFRNRSSGEIL+RL
Sbjct: 481 KQKLYIQVKDSLGFADLTIGNAEVDLGSLQDTVPTDRIVVLGGGWGLFRNRSSGEILVRL 540

Query: 541 TYKAYVEDEEDDKAAASEANVLDIDISDDESSDS---DEPNGAYEKEGEKDAAKETEKES 600
           TYKAYVEDEEDDKAA    + LD DISDDESSDS   DEPNGAY+ EGE DAAKET+KES
Sbjct: 541 TYKAYVEDEEDDKAA---LDALDTDISDDESSDSDEPDEPNGAYD-EGENDAAKETDKES 600

Query: 601 FMDVLAALIVSEEFQGIVASETLNTILQNDA-TSTSTRTTRSSSRSRDAATDTKTKPMVS 660
           FMDVLAALIVSEEFQGIVAS+TLNT LQNDA TS+S  TTR  SRSRD  TD   KP VS
Sbjct: 601 FMDVLAALIVSEEFQGIVASDTLNTKLQNDATTSSSIGTTR--SRSRDVVTD--NKPAVS 660

Query: 661 GNGTGGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           G G  GL ES+LFWLAVITSISVLIAINIGGS+FFNP
Sbjct: 661 GVGDRGLAESSLFWLAVITSISVLIAINIGGSSFFNP 673

BLAST of MS021147 vs. ExPASy Swiss-Prot
Match: Q9UT00 (Uncharacterized protein PYUK71.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAPYUK71.03c PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 6.3e-14
Identity = 96/418 (22.97%), Postives = 171/418 (40.91%), Query Frame = 0

Query: 197 LEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNLKKPDYVERVEIKQFSL 256
           LE D    E+V W+N  L + W ++ P L   +  +   ++     P +++ + + +F+L
Sbjct: 214 LEND---SETVTWLNTFLQQFWYIFEPSLSERITEITDQILSE-NVPSFIDSMALSEFTL 273

Query: 257 GEE----------PLSVRN---VERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG--I 316
           G +          P +  +   ++ R +   ND+    G       +  + L LK G  I
Sbjct: 274 GTKSPRMGFIRSYPKTEEDTVMMDLRLAFSPNDISDLTGREIAACIKPKIALDLKIGKSI 333

Query: 317 IPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSP-------FRL 376
               +PV + D    G L VK++LI   P+   V   F   P   + L P       F +
Sbjct: 334 ASAKMPVLIEDLSFTGNLRVKVKLIDKYPYAKTVGLTFTEKPVFSYILKPLGGDKFGFDI 393

Query: 377 FNLMGM--FLTKLLTEDLPKLFVRPKKIVLDFQK-------GKAVGPVTDEVKSGDMQEG 436
            N+ G+  F+T+ +   L  +   P    LD +          A+G V  +++ GD   G
Sbjct: 394 GNIPGLTTFITEQIHNTLGPMMYSPNVYELDIESMMGAAGLNTALGAVEFKLRKGD---G 453

Query: 437 NKDSVGELSITLVDARKLSYVFYGKTDPYVIL-SLGDQIIRSKKNSQTTVIGPPGEPIWN 496
            KD +G                 G  DPYV++ +  D++I   K +  T     G P++N
Sbjct: 454 FKDGLG-----------------GAVDPYVVIKNSADRVIGKSKVAHNT-----GSPVFN 513

Query: 497 QDFHMLVANPRKQKLYIQVKDSLGF-ADLTVGNAEVDLGSLQD-TVPTDKIVVLGGGWGL 556
           + F+  V N   + L ++V D     +D  +G+A + L +L+   V  D  V L      
Sbjct: 514 ETFYS-VLNSFSENLNLEVYDFNDIRSDKLLGSAVLPLATLEAMPVTNDAFVEL-----T 573

Query: 557 FRNRSSGEILLRLTYKAYVEDEEDDKAAASEANVLDIDISD--DESSDSDEPNGAYEK 579
            + ++ G +   + + A V D  ++        VL   +    + S+D  +   AY K
Sbjct: 574 LKGKTVGRLNYDMKFHAVVPDSGEEITKVDGPGVLQFTVHQCKELSNDPSKRPTAYAK 596

BLAST of MS021147 vs. ExPASy Swiss-Prot
Match: B6ETT4 (Synaptotagmin-2 OS=Arabidopsis thaliana OX=3702 GN=SYT2 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 9.1e-13
Identity = 80/351 (22.79%), Postives = 159/351 (45.30%), Query Frame = 0

Query: 205 ESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNLKKPDY-VERVEIKQFSLGEEPLSV 264
           + ++W+N ++G +W      +      + +P+I   + P+Y ++ VE +  +LG  P S 
Sbjct: 69  DRIDWLNKLIGHMWPYMDKAICKMAKSIAKPIIAE-QIPNYKIDSVEFEMLTLGSLPPSF 128

Query: 265 RNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFGIIPIVVPVGVRDFDIDGELWVKL 324
           + + +  +    ++  ++ +++ G    +++++  FG   +   V V D  +     + L
Sbjct: 129 QGM-KVYATDDKEIIMELSVKWAGNPN-IIVVAKAFG---LKATVQVIDLQVYATPRITL 188

Query: 325 R-LIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLMGMFLTKLLTED---LPKLFVRPKK 384
           + L+P+ P    +  + +  P++ F L              KLL  D   +P L+   ++
Sbjct: 189 KPLVPSFPCFANIFVSLMDKPQVDFGL--------------KLLGADVMAIPGLYRFVQE 248

Query: 385 IVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSITLVDARKLSYV-FYGKTDPYVILS 444
           I+ D      + P T  V+  D  +  K  VG LS+ ++ A KL      G +DPYV L+
Sbjct: 249 IIKDQVANMYLWPKTLNVQIMDPSKAMKKPVGLLSVKVIKAIKLKKKDLLGGSDPYVKLT 308

Query: 445 LGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPRKQKLYIQVKD--SLGFADLTVGN 504
           L    +  KK   T V      P WN++F ++V  P  Q+L + V D   +G  D  +G 
Sbjct: 309 LSGDKVPGKK---TVVKHSNLNPEWNEEFDLVVKEPESQELQLIVYDWEQVGKHD-KIGM 368

Query: 505 AEVDLGSLQDTVP---TDKIVVLGGGWGLFRNRSSGEILLRLTYKAYVEDE 545
             + L  L    P   T +++           +S G++++ + YK + +D+
Sbjct: 369 NVIQLKDLTPEEPKLMTLELLKSMEPKEPVSEKSRGQLVVEVEYKPFKDDD 395

BLAST of MS021147 vs. ExPASy Swiss-Prot
Match: Q7XA06 (Synaptotagmin-3 OS=Arabidopsis thaliana OX=3702 GN=SYT3 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.5e-10
Identity = 68/291 (23.37%), Postives = 133/291 (45.70%), Query Frame = 0

Query: 205 ESVEWVNMVLGKLWKVYRPGLENWLVGLL----QPVIDNLKKPDYVERVEIKQFSLGEEP 264
           E V+W N  +  +W    P L+  + G++    QP+  +      +E +E +  SLG  P
Sbjct: 69  ERVDWFNKFISYMW----PYLDKAVCGIIRSSVQPLFADYIGTFCIESIEFENLSLGTLP 128

Query: 265 LSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFGIIPIVVPVGVRDFDIDGELW 324
            +V  V+   +    +L ++  +++ G   ++L+L     ++ + + V + D      + 
Sbjct: 129 PTVHGVKFYETNE-KELLFEPSIKWAGNPNIVLVLK----VLSLRIRVQLVDLQFFAIVR 188

Query: 325 VKLR-LIPAEPWVGAVSWAFVSLPKIKFELSPF--RLFNLMGM--FLTKLLTEDLPKLFV 384
           V L+ L+P  P  G V  + +  P + F L      L ++ G+  ++ + +   +  ++ 
Sbjct: 189 VALKPLLPTFPCFGMVVVSLMEKPHVDFGLKVLGGDLMSIPGLYRYVQETIKRQVSSMYH 248

Query: 385 RPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSITLVDARK-LSYVFYGKTDPY 444
            P+ + +         P+ D   +       K  VG L ++++ AR  L     G +DPY
Sbjct: 249 WPQVLEI---------PILDSSTA-----SVKKPVGLLHVSILRARNLLKKDLLGTSDPY 308

Query: 445 VILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPRKQKLYIQVKD 486
           V LSL  + + +KK   TT+      P WN+ F ++V +P  Q L ++V D
Sbjct: 309 VKLSLTGEKLPAKK---TTIKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFD 333

BLAST of MS021147 vs. ExPASy Swiss-Prot
Match: P48231 (Tricalbin-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TCB2 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.6e-09
Identity = 60/290 (20.69%), Postives = 123/290 (42.41%), Query Frame = 0

Query: 197 LEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLV-GLLQPVIDNLKKPDYVERVEIKQFS 256
           +EK     ESVEW+N  L K W +  P +   +V G    + +N+  P +++ + + QF+
Sbjct: 158 VEKITSDYESVEWLNTFLDKYWPIIEPSVSQQIVDGTNTALSENVAIPKFIKAIWLDQFT 217

Query: 257 LGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLL-----------MLSLKFGIIPI 316
           LG +P  +  ++   + +++ +   + L +T      L            + LK  I  +
Sbjct: 218 LGVKPPRIDAIKTFQNTKSDVVVMDVCLSFTPHDMSDLDAKQCRNYVNSNVVLKAKIFGM 277

Query: 317 VVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLMGMFLTK 376
            +PV V D      +  + +L+   P V  ++   + +P++ F      +  L+G  +  
Sbjct: 278 DIPVSVADIFFQVFVRFRFQLMTTLPLVETINIQLLEVPEVDF------ISRLLGNSVFN 337

Query: 377 LLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSITLVDA---R 436
                +P L    +K+   +     + P + ++    +       +G L I + +A   R
Sbjct: 338 WEILAIPGLMRLIQKMAFKYLSPVLLPPFSLQLNIPQLLSKTGLPIGVLEIKVKNAHGLR 397

Query: 437 KLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLV 472
           KL  +     DPY+   L  +I+      +T V      P+WN+  ++L+
Sbjct: 398 KLVGMIKKTVDPYLTFELSGKIV-----GKTKVFKNSANPVWNESIYILL 436

BLAST of MS021147 vs. ExPASy Swiss-Prot
Match: Q8LFN9 (Probable ADP-ribosylation factor GTPase-activating protein AGD13 OS=Arabidopsis thaliana OX=3702 GN=AGD13 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.1e-05
Identity = 35/103 (33.98%), Postives = 53/103 (51.46%), Query Frame = 0

Query: 403 QEGNKDSVGELSITLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPI 462
           QEG  + +G L +T+     L+      +DPYV+L+LG      K+  QTTV+     P+
Sbjct: 172 QEGMVEFIGLLKVTIKKGTNLAIRDMMSSDPYVVLNLG------KQKLQTTVMNSNLNPV 231

Query: 463 WNQDFHMLVANPRKQKLYIQVKDSLGF-ADLTVGNAEVDLGSL 505
           WNQ+  ML        + +QV D   F AD  +G A++D+  L
Sbjct: 232 WNQEL-MLSVPESYGPVKLQVYDYDTFSADDIMGEADIDIQPL 267

BLAST of MS021147 vs. ExPASy TrEMBL
Match: A0A6J1DEL6 (tricalbin-3-like OS=Momordica charantia OX=3673 GN=LOC111019736 PE=4 SV=1)

HSP 1 Score: 1335.5 bits (3455), Expect = 0.0e+00
Identity = 684/693 (98.70%), Postives = 684/693 (98.70%), Query Frame = 0

Query: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60
           MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR
Sbjct: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60

Query: 61  RKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQDSSVQVGSNFT 120
           RKWFLACSISPDGASRNLNLDFASS RRGAKSFVVKHIADELEREEFSQDSSVQVGSNFT
Sbjct: 61  RKWFLACSISPDGASRNLNLDFASSTRRGAKSFVVKHIADELEREEFSQDSSVQVGSNFT 120

Query: 121 GFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNED 180
           GFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNED
Sbjct: 121 GFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNED 180

Query: 181 GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNL 240
           GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNL
Sbjct: 181 GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNL 240

Query: 241 KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 300
           KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG
Sbjct: 241 KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 300

Query: 301 IIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM-- 360
           IIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM  
Sbjct: 301 IIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLMAI 360

Query: 361 ---GMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSI 420
               MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSI
Sbjct: 361 PVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSI 420

Query: 421 TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPR 480
           TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPR
Sbjct: 421 TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPR 480

Query: 481 KQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRL 540
           KQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRL
Sbjct: 481 KQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRL 540

Query: 541 TYKAYVEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGEKDAAKETEKESFMD 600
           TYKAYVEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGEKDAAKETEKESFMD
Sbjct: 541 TYKAYVEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGEKDAAKETEKESFMD 600

Query: 601 VLAALIVSEEFQGIVASETLNTILQNDATSTSTRTTRSSSRSRDAATDTKTKPMVSGNGT 660
           VLAALIVSEEFQGIV SETLNTILQNDATSTSTRTTRSSSRSRDAATDTKTKPMVSGNGT
Sbjct: 601 VLAALIVSEEFQGIVTSETLNTILQNDATSTSTRTTRSSSRSRDAATDTKTKPMVSGNGT 660

Query: 661 GGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           GGLTESALFWLA ITSISVLIAINIGGSNFFNP
Sbjct: 661 GGLTESALFWLAAITSISVLIAINIGGSNFFNP 693

BLAST of MS021147 vs. ExPASy TrEMBL
Match: A0A5D3CV29 (Tricalbin-3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002750 PE=4 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 586/696 (84.20%), Postives = 617/696 (88.65%), Query Frame = 0

Query: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60
           MI HF   +F FSNSFSQHCPCNNFT F            P SRK RK FS++     FR
Sbjct: 1   MITHFPFPTFDFSNSFSQHCPCNNFTAF-----------VPFSRKSRKPFSSSL---NFR 60

Query: 61  RKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQ-DSSVQVGSNF 120
           R+WFL CS+SPDG + N NL+FA+SARRG ++FVVK I++ELE EEFSQ +SSVQVGSNF
Sbjct: 61  RRWFLVCSLSPDGVTSNFNLEFATSARRGVRNFVVKRISNELEGEEFSQEESSVQVGSNF 120

Query: 121 TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNE 180
           TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGV FDKLWTFR+R+KSRN+
Sbjct: 121 TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVAFDKLWTFRKRSKSRND 180

Query: 181 DGRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDN 240
           DGR GTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPG+E+WLVGLLQPVIDN
Sbjct: 181 DGRLGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGIEDWLVGLLQPVIDN 240

Query: 241 LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF 300
           LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF
Sbjct: 241 LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF 300

Query: 301 GIIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM- 360
           GIIPIVVPV VRDFDIDGELWVKLRLIP EPWVGAVSWAFVSLPKIKFELSPFRLFNLM 
Sbjct: 301 GIIPIVVPVVVRDFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKFELSPFRLFNLMA 360

Query: 361 ----GMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELS 420
                MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPV DEVKSG MQEGN D VGELS
Sbjct: 361 IPVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVPDEVKSGVMQEGNNDFVGELS 420

Query: 421 ITLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANP 480
           +TLVDARKLSY+FYGKTDPYV+LSLGDQ IRSKKNSQTTVIGPPGEPIWNQDFHMLVANP
Sbjct: 421 VTLVDARKLSYLFYGKTDPYVVLSLGDQTIRSKKNSQTTVIGPPGEPIWNQDFHMLVANP 480

Query: 481 RKQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLR 540
           RKQKLYIQVKDSLGFADLT+GNAEVDLGSLQDTVPTD IVVLGGGWG FRNRSSGE+L+R
Sbjct: 481 RKQKLYIQVKDSLGFADLTIGNAEVDLGSLQDTVPTDTIVVLGGGWGFFRNRSSGEVLVR 540

Query: 541 LTYKAYVEDEEDDKAAASEANVLDIDIS-DDESSDSDEPNGAYEKEGEKDAAKETEKESF 600
           LTYKAYVEDEEDDKAA   +N LDIDIS DDESSD+DEPNGAYE E E D  K T KESF
Sbjct: 541 LTYKAYVEDEEDDKAA---SNALDIDISDDDESSDTDEPNGAYE-ESENDTVKATGKESF 600

Query: 601 MDVLAALIVSEEFQGIVASETLNTILQNDAT-STSTRTTRSSSRSRDAATDTKTKPMVSG 660
           MDVLAALIVSEEF GIVAS+ LNT LQNDAT STS+ TT  +SRSRD ATD   KP VS 
Sbjct: 601 MDVLAALIVSEEFLGIVASDALNTKLQNDATLSTSSGTT--NSRSRDTATD--NKPTVSS 660

Query: 661 NGTGGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           NG GGL +SALFWL VITSISVLIAINIGGS+FFNP
Sbjct: 661 NGAGGLADSALFWLTVITSISVLIAINIGGSSFFNP 674

BLAST of MS021147 vs. ExPASy TrEMBL
Match: A0A1S3BEM3 (tricalbin-3-like OS=Cucumis melo OX=3656 GN=LOC103488839 PE=4 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 586/696 (84.20%), Postives = 617/696 (88.65%), Query Frame = 0

Query: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60
           MI HF   +F FSNSFSQHCPCNNFT F            P SRK RK FS++     FR
Sbjct: 1   MITHFPFPTFDFSNSFSQHCPCNNFTAF-----------VPFSRKSRKPFSSSL---NFR 60

Query: 61  RKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQ-DSSVQVGSNF 120
           R+WFL CS+SPDG + N NL+FA+SARRG ++FVVK I++ELE EEFSQ +SSVQVGSNF
Sbjct: 61  RRWFLVCSLSPDGVTSNFNLEFATSARRGVRNFVVKRISNELEGEEFSQEESSVQVGSNF 120

Query: 121 TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNE 180
           TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGV FDKLWTFR+R+KSRN+
Sbjct: 121 TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVAFDKLWTFRKRSKSRND 180

Query: 181 DGRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDN 240
           DGR GTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPG+E+WLVGLLQPVIDN
Sbjct: 181 DGRLGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGIEDWLVGLLQPVIDN 240

Query: 241 LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF 300
           LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF
Sbjct: 241 LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF 300

Query: 301 GIIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM- 360
           GIIPIVVPV VRDFDIDGELWVKLRLIP EPWVGAVSWAFVSLPKIKFELSPFRLFNLM 
Sbjct: 301 GIIPIVVPVVVRDFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKFELSPFRLFNLMA 360

Query: 361 ----GMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELS 420
                MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPV DEVKSG MQEGN D VGELS
Sbjct: 361 IPVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVPDEVKSGVMQEGNNDFVGELS 420

Query: 421 ITLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANP 480
           +TLVDARKLSY+FYGKTDPYV+LSLGDQ IRSKKNSQTTVIGPPGEPIWNQDFHMLVANP
Sbjct: 421 VTLVDARKLSYLFYGKTDPYVVLSLGDQTIRSKKNSQTTVIGPPGEPIWNQDFHMLVANP 480

Query: 481 RKQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLR 540
           RKQKLYIQVKDSLGFADLT+GNAEVDLGSLQDTVPTD IVVLGGGWG FRNRSSGE+L+R
Sbjct: 481 RKQKLYIQVKDSLGFADLTIGNAEVDLGSLQDTVPTDTIVVLGGGWGFFRNRSSGEVLVR 540

Query: 541 LTYKAYVEDEEDDKAAASEANVLDIDIS-DDESSDSDEPNGAYEKEGEKDAAKETEKESF 600
           LTYKAYVEDEEDDKAA   +N LDIDIS DDESSD+DEPNGAYE E E D  K T KESF
Sbjct: 541 LTYKAYVEDEEDDKAA---SNALDIDISDDDESSDTDEPNGAYE-ESENDTVKATGKESF 600

Query: 601 MDVLAALIVSEEFQGIVASETLNTILQNDAT-STSTRTTRSSSRSRDAATDTKTKPMVSG 660
           MDVLAALIVSEEF GIVAS+ LNT LQNDAT STS+ TT  +SRSRD ATD   KP VS 
Sbjct: 601 MDVLAALIVSEEFLGIVASDALNTKLQNDATLSTSSGTT--NSRSRDTATD--NKPTVSS 660

Query: 661 NGTGGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           NG GGL +SALFWL VITSISVLIAINIGGS+FFNP
Sbjct: 661 NGAGGLADSALFWLTVITSISVLIAINIGGSSFFNP 674

BLAST of MS021147 vs. ExPASy TrEMBL
Match: A0A0A0KV67 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G585990 PE=4 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 578/696 (83.05%), Postives = 610/696 (87.64%), Query Frame = 0

Query: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60
           MI HF   +F FSNSFSQHCPCNNFT F            P SRK RK FS+      FR
Sbjct: 1   MITHFPFPTFDFSNSFSQHCPCNNFTAF-----------VPFSRKSRKPFSSTLT---FR 60

Query: 61  RKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQ-DSSVQVGSNF 120
           R+WFL CS+SPDG + N +L+FA+SARRG ++FVV  I++ELE EEFSQ +SSVQVGSNF
Sbjct: 61  RRWFLVCSLSPDGVTSNFDLEFATSARRGVRNFVVNRISNELEGEEFSQEESSVQVGSNF 120

Query: 121 TGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNE 180
           TGFQEDPIVDKLRTQLG IHPIPSPPINRNIVGLFVFFFFVGV FDKLWTFR+R+KSRN 
Sbjct: 121 TGFQEDPIVDKLRTQLGAIHPIPSPPINRNIVGLFVFFFFVGVAFDKLWTFRKRSKSRNN 180

Query: 181 DGRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDN 240
           DGR GTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPG+E+WLVGLLQPVIDN
Sbjct: 181 DGRLGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGIEDWLVGLLQPVIDN 240

Query: 241 LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF 300
           LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF
Sbjct: 241 LKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKF 300

Query: 301 GIIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM- 360
           GIIPIVVPV VRDFDIDGELWVKLRLIP EPWVGAVSWAFVSLPKIKFELSPFRLFNLM 
Sbjct: 301 GIIPIVVPVVVRDFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKFELSPFRLFNLMA 360

Query: 361 ----GMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELS 420
                MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPV DEVKSG MQEGN D VGELS
Sbjct: 361 IPVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVPDEVKSGVMQEGNNDFVGELS 420

Query: 421 ITLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANP 480
           +TLVDARKLSY+FYGKTDPYV+LSLGDQ IRSKKNSQTTVIGPPGEPIWNQDFHMLVANP
Sbjct: 421 VTLVDARKLSYLFYGKTDPYVVLSLGDQTIRSKKNSQTTVIGPPGEPIWNQDFHMLVANP 480

Query: 481 RKQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLR 540
           RKQKLYIQVKDSLGFADLT+GNAEVDLGSLQDTVPTD IVVL  GWGLFRNRSSGE+L+R
Sbjct: 481 RKQKLYIQVKDSLGFADLTIGNAEVDLGSLQDTVPTDTIVVLREGWGLFRNRSSGEVLVR 540

Query: 541 LTYKAYVEDEEDDKAAASEANVLDIDIS-DDESSDSDEPNGAYEKEGEKDAAKETEKESF 600
           LTYKAYVEDEEDDKAA   ++ LDIDIS DDESSD+DEPNG YE E E D  K T KESF
Sbjct: 541 LTYKAYVEDEEDDKAA---SDALDIDISDDDESSDTDEPNGVYE-ESENDGVKATGKESF 600

Query: 601 MDVLAALIVSEEFQGIVASETLNTILQNDAT-STSTRTTRSSSRSRDAATDTKTKPMVSG 660
           MDVLAALIVSEEF GIVAS+ LNT LQND T STS+ TT  +SRSRD A D   KP VS 
Sbjct: 601 MDVLAALIVSEEFLGIVASDALNTKLQNDPTISTSSGTT--NSRSRDTAID--NKPTVSS 660

Query: 661 NGTGGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           NG+GGL +SALFWL VITSISVLIAINIGGS+FFNP
Sbjct: 661 NGSGGLADSALFWLTVITSISVLIAINIGGSSFFNP 674

BLAST of MS021147 vs. ExPASy TrEMBL
Match: A0A6J1GYB4 (tricalbin-3-like OS=Cucurbita moschata OX=3662 GN=LOC111458609 PE=4 SV=1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 579/697 (83.07%), Postives = 606/697 (86.94%), Query Frame = 0

Query: 1   MIMHFTSASFGFSNSFSQHCPCNNFTVFTPSHTTPNPLSTPLSRKRRKQFSAAFVHRYFR 60
           MI H TS  F  SNSF QH P +NF                ++  R    +AAF+ R  R
Sbjct: 1   MITHSTSPPFDSSNSFPQHSPSHNF----------------ITGARNSLSTAAFLPRNPR 60

Query: 61  RKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQDSSVQVGSNFT 120
           RK F   S+SPD A+R+ NL FA+ ARR A  FVVK   +ELEREEFSQDSSVQVGS+FT
Sbjct: 61  RKRFFPSSLSPDAATRHFNLQFATLARRSANFFVVKRTTNELEREEFSQDSSVQVGSSFT 120

Query: 121 GFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNED 180
            FQEDPIVDKLRTQLGVIHPIPSPPINRNIV LFVFFFFVGV FDKLWT R+R+KSRNED
Sbjct: 121 AFQEDPIVDKLRTQLGVIHPIPSPPINRNIVALFVFFFFVGVAFDKLWTLRKRSKSRNED 180

Query: 181 GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNL 240
           GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLE+WLVGLLQPVIDNL
Sbjct: 181 GRRGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLEDWLVGLLQPVIDNL 240

Query: 241 KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 300
           KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG
Sbjct: 241 KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 300

Query: 301 IIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLM-- 360
           IIPI VPVGVR+FDIDGELWVKLRLIP EPWVGAVSWAFVSLPKIKFELSPFRLFNLM  
Sbjct: 301 IIPITVPVGVRNFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKFELSPFRLFNLMAI 360

Query: 361 ---GMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSI 420
               MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPV DEVKSGD+QEGNK  VGELS+
Sbjct: 361 PVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVPDEVKSGDIQEGNKGFVGELSV 420

Query: 421 TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPR 480
           TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIG PGEPIWNQDFHMLVANPR
Sbjct: 421 TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGSPGEPIWNQDFHMLVANPR 480

Query: 481 KQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRL 540
           KQKLYIQVKDSLGFADLT+GNAEVDLGSLQDTVPTD+IVVLGGGWGLFRNRSSGEIL+RL
Sbjct: 481 KQKLYIQVKDSLGFADLTIGNAEVDLGSLQDTVPTDRIVVLGGGWGLFRNRSSGEILVRL 540

Query: 541 TYKAYVEDEEDDKAAASEANVLDIDISDDESSDS---DEPNGAYEKEGEKDAAKETEKES 600
           TYKAYVEDEEDDKAA    + LD DISDDESSDS   DEPNGAY+ EGE DAAKET+KES
Sbjct: 541 TYKAYVEDEEDDKAA---LDALDTDISDDESSDSDEPDEPNGAYD-EGENDAAKETDKES 600

Query: 601 FMDVLAALIVSEEFQGIVASETLNTILQNDA-TSTSTRTTRSSSRSRDAATDTKTKPMVS 660
           FMDVLAALIVSEEFQGIVAS+TLNT LQNDA TS+S  TTR  SRSRD  TD   KP VS
Sbjct: 601 FMDVLAALIVSEEFQGIVASDTLNTKLQNDATTSSSIGTTR--SRSRDVVTD--NKPAVS 660

Query: 661 GNGTGGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           G G  GL ES+LFWLAVITSISVLIAINIGGS+FFNP
Sbjct: 661 GVGDRGLAESSLFWLAVITSISVLIAINIGGSSFFNP 673

BLAST of MS021147 vs. TAIR 10
Match: AT3G19830.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 810.1 bits (2091), Expect = 1.4e-234
Identity = 460/718 (64.07%), Postives = 540/718 (75.21%), Query Frame = 0

Query: 1   MIMHFTS--ASFGFSNSFSQH--CPCNN---FTVFTPSHTTPNPLSTPLSRKRRKQFSAA 60
           MI+  +S  +SF F +  S+   CPC+N     VF+   T           KRR+     
Sbjct: 1   MILQSSSSCSSFDFPSFVSRRLLCPCSNEHGLIVFSDGFT-----------KRRRILRR- 60

Query: 61  FVHRYFRRKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREE---FSQD 120
            VH       F++  I  D  S+N+ L  A SARR A+S VV   ++E E EE    SQ+
Sbjct: 61  -VHAANSNSRFVSSGIRTD--SKNIGL--ADSARRAARSLVVTRFSNEFEDEEASSSSQE 120

Query: 121 SSVQVG-SNFTGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWT 180
           S++Q   +NFT F+EDPIVDKLRTQLGVIHPIPSPPI+RN +GLF FFFFVGV+ DKLWT
Sbjct: 121 SAIQGDRNNFTNFREDPIVDKLRTQLGVIHPIPSPPISRNAIGLFAFFFFVGVICDKLWT 180

Query: 181 FRRRNKSRNEDGRR---GTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLE 240
           +R+R +    DG +   G W QVPTSFS  LEKDLQRKESVEWVNMVL KLWKVYR G+E
Sbjct: 181 WRKRRRQMAGDGGQRGAGPWAQVPTSFSLSLEKDLQRKESVEWVNMVLVKLWKVYRGGIE 240

Query: 241 NWLVGLLQPVIDNLKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYT 300
           NWLVGLLQPVID+LKKPDYV+RVEIKQFSLG+EPLSVRNVERRTSRR NDLQYQIGLRYT
Sbjct: 241 NWLVGLLQPVIDDLKKPDYVQRVEIKQFSLGDEPLSVRNVERRTSRRVNDLQYQIGLRYT 300

Query: 301 GGARMLLMLSLKFGIIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIK 360
           GGARMLLMLSLKFGIIP+VVPVG+RDFDIDGELWVKLRLIP+ PWVGA SWAFVSLPKIK
Sbjct: 301 GGARMLLMLSLKFGIIPVVVPVGIRDFDIDGELWVKLRLIPSAPWVGAASWAFVSLPKIK 360

Query: 361 FELSPFRLFNLMG-----MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGD 420
           FEL+PFRLFNLMG     MFLTKLLTEDLP+LFVRPKKIVLDFQKGKAVGPV++++KSG+
Sbjct: 361 FELAPFRLFNLMGIPVLSMFLTKLLTEDLPRLFVRPKKIVLDFQKGKAVGPVSEDLKSGE 420

Query: 421 MQEGNKDSVGELSITLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEP 480
           MQEGNKD VGELS+TLV+A+KL Y+F G+TDPYVIL +GDQ+IRSKKNSQTTVIG PG+P
Sbjct: 421 MQEGNKDFVGELSVTLVNAQKLPYMFSGRTDPYVILRIGDQVIRSKKNSQTTVIGAPGQP 480

Query: 481 IWNQDFHMLVANPRKQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWG 540
           IWNQDF  LV+NPR+Q L I+V D LGFAD+ +G  EVDL SL DTVPTD+ V L GGW 
Sbjct: 481 IWNQDFQFLVSNPREQVLQIEVNDCLGFADMAIGIGEVDLESLPDTVPTDRFVSLRGGWS 540

Query: 541 LFRNRSSGEILLRLTYKAYVEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGE 600
           LF   S+GEILLRLTYKAYVEDEEDDK     A  +  D SDDE SDS+EP+     + +
Sbjct: 541 LFGKGSTGEILLRLTYKAYVEDEEDDK---RNAKAIYADASDDEMSDSEEPSSFV--QND 600

Query: 601 KDAAKETEKESFMDVLAALIVSEEFQGIVASETLNTILQNDATSTSTRTTR----SSSRS 660
           K  + +  +ESFM+VL+ALI+SEEFQGIV+SET N  + +  +S S   +     S SR 
Sbjct: 601 KIPSDDIGQESFMNVLSALILSEEFQGIVSSETGNNKVDDGESSVSPVPSMSGADSESRP 660

Query: 661 RDA----ATDTKTKPMVSGNGT---GGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           +DA     +D + K   S  G+   GGL   AL W  VITS+ VL+AIN+GGS+FFNP
Sbjct: 661 KDAGNGDVSDLEVKNAKSDRGSINNGGL---ALLWFGVITSVLVLVAINMGGSSFFNP 693

BLAST of MS021147 vs. TAIR 10
Match: AT3G19830.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 810.1 bits (2091), Expect = 1.4e-234
Identity = 460/718 (64.07%), Postives = 540/718 (75.21%), Query Frame = 0

Query: 1   MIMHFTS--ASFGFSNSFSQH--CPCNN---FTVFTPSHTTPNPLSTPLSRKRRKQFSAA 60
           MI+  +S  +SF F +  S+   CPC+N     VF+   T           KRR+     
Sbjct: 1   MILQSSSSCSSFDFPSFVSRRLLCPCSNEHGLIVFSDGFT-----------KRRRILRR- 60

Query: 61  FVHRYFRRKWFLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREE---FSQD 120
            VH       F++  I  D  S+N+ L  A SARR A+S VV   ++E E EE    SQ+
Sbjct: 61  -VHAANSNSRFVSSGIRTD--SKNIGL--ADSARRAARSLVVTRFSNEFEDEEASSSSQE 120

Query: 121 SSVQVG-SNFTGFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWT 180
           S++Q   +NFT F+EDPIVDKLRTQLGVIHPIPSPPI+RN +GLF FFFFVGV+ DKLWT
Sbjct: 121 SAIQGDRNNFTNFREDPIVDKLRTQLGVIHPIPSPPISRNAIGLFAFFFFVGVICDKLWT 180

Query: 181 FRRRNKSRNEDGRR---GTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLE 240
           +R+R +    DG +   G W QVPTSFS  LEKDLQRKESVEWVNMVL KLWKVYR G+E
Sbjct: 181 WRKRRRQMAGDGGQRGAGPWAQVPTSFSLSLEKDLQRKESVEWVNMVLVKLWKVYRGGIE 240

Query: 241 NWLVGLLQPVIDNLKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYT 300
           NWLVGLLQPVID+LKKPDYV+RVEIKQFSLG+EPLSVRNVERRTSRR NDLQYQIGLRYT
Sbjct: 241 NWLVGLLQPVIDDLKKPDYVQRVEIKQFSLGDEPLSVRNVERRTSRRVNDLQYQIGLRYT 300

Query: 301 GGARMLLMLSLKFGIIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIK 360
           GGARMLLMLSLKFGIIP+VVPVG+RDFDIDGELWVKLRLIP+ PWVGA SWAFVSLPKIK
Sbjct: 301 GGARMLLMLSLKFGIIPVVVPVGIRDFDIDGELWVKLRLIPSAPWVGAASWAFVSLPKIK 360

Query: 361 FELSPFRLFNLMG-----MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGD 420
           FEL+PFRLFNLMG     MFLTKLLTEDLP+LFVRPKKIVLDFQKGKAVGPV++++KSG+
Sbjct: 361 FELAPFRLFNLMGIPVLSMFLTKLLTEDLPRLFVRPKKIVLDFQKGKAVGPVSEDLKSGE 420

Query: 421 MQEGNKDSVGELSITLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEP 480
           MQEGNKD VGELS+TLV+A+KL Y+F G+TDPYVIL +GDQ+IRSKKNSQTTVIG PG+P
Sbjct: 421 MQEGNKDFVGELSVTLVNAQKLPYMFSGRTDPYVILRIGDQVIRSKKNSQTTVIGAPGQP 480

Query: 481 IWNQDFHMLVANPRKQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWG 540
           IWNQDF  LV+NPR+Q L I+V D LGFAD+ +G  EVDL SL DTVPTD+ V L GGW 
Sbjct: 481 IWNQDFQFLVSNPREQVLQIEVNDCLGFADMAIGIGEVDLESLPDTVPTDRFVSLRGGWS 540

Query: 541 LFRNRSSGEILLRLTYKAYVEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGE 600
           LF   S+GEILLRLTYKAYVEDEEDDK     A  +  D SDDE SDS+EP+     + +
Sbjct: 541 LFGKGSTGEILLRLTYKAYVEDEEDDK---RNAKAIYADASDDEMSDSEEPSSFV--QND 600

Query: 601 KDAAKETEKESFMDVLAALIVSEEFQGIVASETLNTILQNDATSTSTRTTR----SSSRS 660
           K  + +  +ESFM+VL+ALI+SEEFQGIV+SET N  + +  +S S   +     S SR 
Sbjct: 601 KIPSDDIGQESFMNVLSALILSEEFQGIVSSETGNNKVDDGESSVSPVPSMSGADSESRP 660

Query: 661 RDA----ATDTKTKPMVSGNGT---GGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           +DA     +D + K   S  G+   GGL   AL W  VITS+ VL+AIN+GGS+FFNP
Sbjct: 661 KDAGNGDVSDLEVKNAKSDRGSINNGGL---ALLWFGVITSVLVLVAINMGGSSFFNP 693

BLAST of MS021147 vs. TAIR 10
Match: AT1G50260.1 (N-terminal-transmembrane-C2 domain type 5.1 )

HSP 1 Score: 805.4 bits (2079), Expect = 3.4e-233
Identity = 428/637 (67.19%), Postives = 507/637 (79.59%), Query Frame = 0

Query: 64  FLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQDSSVQVGSNFTGFQ 123
           F++C     G S+NL L    S+R+ A  FV+    +E E E  S++S+ Q  +NF+ F+
Sbjct: 52  FISCGFR--GNSKNLRL--TDSSRKAANRFVIARFTNEFEDEGSSKESNDQ--ANFSNFR 111

Query: 124 EDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNEDGRR 183
           EDPIVDKLRTQLGVIHP+PSP INR+++ LFVFFFFVGV FDKLWT+R+R +    DG +
Sbjct: 112 EDPIVDKLRTQLGVIHPLPSPTINRSVISLFVFFFFVGVAFDKLWTWRKRQRETGGDGNQ 171

Query: 184 ---GTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNL 243
              G WPQVPTSFS FLEKDLQRKESVEWVNMVLGKLWKVYR G+ENWLVGLLQPVID+L
Sbjct: 172 RGVGPWPQVPTSFSLFLEKDLQRKESVEWVNMVLGKLWKVYRAGIENWLVGLLQPVIDDL 231

Query: 244 KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 303
           KKPDYV+RVEIKQFSLG+EPLSVRNVER+TSRRANDLQYQIGLRYTGGARMLLMLSLKFG
Sbjct: 232 KKPDYVQRVEIKQFSLGDEPLSVRNVERKTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 291

Query: 304 IIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLMG- 363
           +IPIVVPVGVRDFDIDGELWVKLRLIP +PWVGAVS +FVSLPK+ F+L+ FRLFNLMG 
Sbjct: 292 VIPIVVPVGVRDFDIDGELWVKLRLIPTQPWVGAVSCSFVSLPKVTFQLAAFRLFNLMGI 351

Query: 364 ----MFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSI 423
               MFLTKLLT DLP+LFVRPKKIVLDFQKGKAVGPV +++KSG+MQEGNKD VGELS+
Sbjct: 352 PVLSMFLTKLLTVDLPRLFVRPKKIVLDFQKGKAVGPVLEDLKSGEMQEGNKDFVGELSV 411

Query: 424 TLVDARKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPR 483
           TLVDA+KL Y+F+GKTDPY IL LGDQ+IRSK+NSQTTVIG PG+PIWNQDF  LV+NPR
Sbjct: 412 TLVDAQKLRYMFFGKTDPYAILRLGDQVIRSKRNSQTTVIGAPGQPIWNQDFQFLVSNPR 471

Query: 484 KQKLYIQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRL 543
           +Q L I+V D LGFAD+ +G  EVDL  LQDTVPTD+IVVL GGW LF   S+GEILLRL
Sbjct: 472 EQVLQIEVNDRLGFADMAIGTGEVDLRFLQDTVPTDRIVVLRGGWSLFGKGSAGEILLRL 531

Query: 544 TYKAYVEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGEKDAAKETEKESFMD 603
           TYK+YVE+EEDDK      NV  ID SDDE SDS+E  G++ ++G K ++ + ++ESFM+
Sbjct: 532 TYKSYVEEEEDDK-----TNVKAIDTSDDEMSDSEE-LGSFVRKG-KLSSDDIDQESFMN 591

Query: 604 VLAALIVSEEFQGIVASETLNTILQ-NDATSTSTRTTRSSSRSRDAATDTKTKPMV---S 663
           VL+ALIVSEEFQGIV+SE  + I+   D+      +   +S+  +   D     ++   S
Sbjct: 592 VLSALIVSEEFQGIVSSEARDGIIDGGDSLGAPVPSKPDTSKGSERNADVSNLDLLVANS 651

Query: 664 GNGTGGLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           G G GG    AL W  +IT I VL+AIN+ GS+FFNP
Sbjct: 652 GRGAGGDGGLALLWFGIITGILVLVAINMEGSSFFNP 675

BLAST of MS021147 vs. TAIR 10
Match: AT1G50260.2 (N-terminal-transmembrane-C2 domain type 5.1 )

HSP 1 Score: 730.7 bits (1885), Expect = 1.1e-210
Identity = 395/632 (62.50%), Postives = 473/632 (74.84%), Query Frame = 0

Query: 64  FLACSISPDGASRNLNLDFASSARRGAKSFVVKHIADELEREEFSQDSSVQVGSNFTGFQ 123
           F++C     G S+NL L    S+R+ A  FV+    +E E E  S++S+ Q  +NF+ F+
Sbjct: 52  FISCGFR--GNSKNLRL--TDSSRKAANRFVIARFTNEFEDEGSSKESNDQ--ANFSNFR 111

Query: 124 EDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVVFDKLWTFRRRNKSRNEDGRR 183
           EDPIVDKLRTQLGVIHP+PSP INR+++ LFVFFFFVGV FDKLWT+R+R +    DG +
Sbjct: 112 EDPIVDKLRTQLGVIHPLPSPTINRSVISLFVFFFFVGVAFDKLWTWRKRQRETGGDGNQ 171

Query: 184 ---GTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGLENWLVGLLQPVIDNL 243
              G WPQVPTSFS FLEKDLQRKESVEWVNMVLGKLWKVYR G+ENWLVGLLQPVID+L
Sbjct: 172 RGVGPWPQVPTSFSLFLEKDLQRKESVEWVNMVLGKLWKVYRAGIENWLVGLLQPVIDDL 231

Query: 244 KKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 303
           KKPDYV+RVEIKQFSLG+EPLSVRNVER+TSRRANDLQYQIGLRYTGGARMLLMLSLKFG
Sbjct: 232 KKPDYVQRVEIKQFSLGDEPLSVRNVERKTSRRANDLQYQIGLRYTGGARMLLMLSLKFG 291

Query: 304 IIPIVVPVGVRDFDIDGELWVKLRLIPAEPWVGAVSWAFVSLPKIKFELSPFRLFNLMGM 363
           +IPIVVPVGVRDFDIDGELWVKLRLIP +PWVGAVS +FVSLPK+ F+L+ FRLFNLM  
Sbjct: 292 VIPIVVPVGVRDFDIDGELWVKLRLIPTQPWVGAVSCSFVSLPKVTFQLAAFRLFNLM-- 351

Query: 364 FLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSITLVDA 423
                                             +++KSG+MQEGNKD VGELS+TLVDA
Sbjct: 352 ----------------------------------EDLKSGEMQEGNKDFVGELSVTLVDA 411

Query: 424 RKLSYVFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPRKQKLY 483
           +KL Y+F+GKTDPY IL LGDQ+IRSK+NSQTTVIG PG+PIWNQDF  LV+NPR+Q L 
Sbjct: 412 QKLRYMFFGKTDPYAILRLGDQVIRSKRNSQTTVIGAPGQPIWNQDFQFLVSNPREQVLQ 471

Query: 484 IQVKDSLGFADLTVGNAEVDLGSLQDTVPTDKIVVLGGGWGLFRNRSSGEILLRLTYKAY 543
           I+V D LGFAD+ +G  EVDL  LQDTVPTD+IVVL GGW LF   S+GEILLRLTYK+Y
Sbjct: 472 IEVNDRLGFADMAIGTGEVDLRFLQDTVPTDRIVVLRGGWSLFGKGSAGEILLRLTYKSY 531

Query: 544 VEDEEDDKAAASEANVLDIDISDDESSDSDEPNGAYEKEGEKDAAKETEKESFMDVLAAL 603
           VE+EEDDK      NV  ID SDDE SDS+E  G++ ++G K ++ + ++ESFM+VL+AL
Sbjct: 532 VEEEEDDK-----TNVKAIDTSDDEMSDSEE-LGSFVRKG-KLSSDDIDQESFMNVLSAL 591

Query: 604 IVSEEFQGIVASETLNTILQ-NDATSTSTRTTRSSSRSRDAATDTKTKPMV---SGNGTG 663
           IVSEEFQGIV+SE  + I+   D+      +   +S+  +   D     ++   SG G G
Sbjct: 592 IVSEEFQGIVSSEARDGIIDGGDSLGAPVPSKPDTSKGSERNADVSNLDLLVANSGRGAG 634

Query: 664 GLTESALFWLAVITSISVLIAINIGGSNFFNP 689
           G    AL W  +IT I VL+AIN+ GS+FFNP
Sbjct: 652 GDGGLALLWFGIITGILVLVAINMEGSSFFNP 634

BLAST of MS021147 vs. TAIR 10
Match: AT5G04220.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 68.9 bits (167), Expect = 1.8e-11
Identity = 68/291 (23.37%), Postives = 133/291 (45.70%), Query Frame = 0

Query: 205 ESVEWVNMVLGKLWKVYRPGLENWLVGLL----QPVIDNLKKPDYVERVEIKQFSLGEEP 264
           E V+W N  +  +W    P L+  + G++    QP+  +      +E +E +  SLG  P
Sbjct: 69  ERVDWFNKFISYMW----PYLDKAVCGIIRSSVQPLFADYIGTFCIESIEFENLSLGTLP 128

Query: 265 LSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLSLKFGIIPIVVPVGVRDFDIDGELW 324
            +V  V+   +    +L ++  +++ G   ++L+L     ++ + + V + D      + 
Sbjct: 129 PTVHGVKFYETNE-KELLFEPSIKWAGNPNIVLVLK----VLSLRIRVQLVDLQFFAIVR 188

Query: 325 VKLR-LIPAEPWVGAVSWAFVSLPKIKFELSPF--RLFNLMGM--FLTKLLTEDLPKLFV 384
           V L+ L+P  P  G V  + +  P + F L      L ++ G+  ++ + +   +  ++ 
Sbjct: 189 VALKPLLPTFPCFGMVVVSLMEKPHVDFGLKVLGGDLMSIPGLYRYVQETIKRQVSSMYH 248

Query: 385 RPKKIVLDFQKGKAVGPVTDEVKSGDMQEGNKDSVGELSITLVDARK-LSYVFYGKTDPY 444
            P+ + +         P+ D   +       K  VG L ++++ AR  L     G +DPY
Sbjct: 249 WPQVLEI---------PILDSSTA-----SVKKPVGLLHVSILRARNLLKKDLLGTSDPY 308

Query: 445 VILSLGDQIIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPRKQKLYIQVKD 486
           V LSL  + + +KK   TT+      P WN+ F ++V +P  Q L ++V D
Sbjct: 309 VKLSLTGEKLPAKK---TTIKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFD 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151857.10.0e+0098.70tricalbin-3-like [Momordica charantia][more]
XP_038892158.10.0e+0085.78tricalbin-3-like [Benincasa hispida][more]
XP_008445968.10.0e+0084.20PREDICTED: tricalbin-3-like [Cucumis melo] >TYK15771.1 tricalbin-3-like [Cucumis... [more]
XP_004135352.10.0e+0083.05tricalbin-3 [Cucumis sativus] >KGN51626.1 hypothetical protein Csa_008247 [Cucum... [more]
KAG6601632.10.0e+0083.21Extended synaptotagmin-1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9UT006.3e-1422.97Uncharacterized protein PYUK71.03c OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
B6ETT49.1e-1322.79Synaptotagmin-2 OS=Arabidopsis thaliana OX=3702 GN=SYT2 PE=2 SV=1[more]
Q7XA062.5e-1023.37Synaptotagmin-3 OS=Arabidopsis thaliana OX=3702 GN=SYT3 PE=2 SV=1[more]
P482313.6e-0920.69Tricalbin-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 G... [more]
Q8LFN94.1e-0533.98Probable ADP-ribosylation factor GTPase-activating protein AGD13 OS=Arabidopsis ... [more]
Match NameE-valueIdentityDescription
A0A6J1DEL60.0e+0098.70tricalbin-3-like OS=Momordica charantia OX=3673 GN=LOC111019736 PE=4 SV=1[more]
A0A5D3CV290.0e+0084.20Tricalbin-3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G0027... [more]
A0A1S3BEM30.0e+0084.20tricalbin-3-like OS=Cucumis melo OX=3656 GN=LOC103488839 PE=4 SV=1[more]
A0A0A0KV670.0e+0083.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G585990 PE=4 SV=1[more]
A0A6J1GYB40.0e+0083.07tricalbin-3-like OS=Cucurbita moschata OX=3662 GN=LOC111458609 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G19830.11.4e-23464.07Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT3G19830.21.4e-23464.07Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT1G50260.13.4e-23367.19N-terminal-transmembrane-C2 domain type 5.1 [more]
AT1G50260.21.1e-21062.50N-terminal-transmembrane-C2 domain type 5.1 [more]
AT5G04220.21.8e-1123.37Calcium-dependent lipid-binding (CaLB domain) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000008C2 domainSMARTSM00239C2_3ccoord: 412..513
e-value: 5.2E-9
score: 45.9
IPR000008C2 domainPFAMPF00168C2coord: 411..509
e-value: 1.3E-12
score: 47.8
IPR000008C2 domainPROSITEPS50004C2coord: 392..514
score: 14.171391
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 392..540
e-value: 3.3E-18
score: 67.9
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 411..539
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 551..588
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 623..653
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 571..588
NoneNo IPR availablePANTHERPTHR47261CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEINcoord: 12..688
NoneNo IPR availableCDDcd00030C2coord: 413..507
e-value: 1.31871E-11
score: 59.7731
IPR031468Synaptotagmin-like mitochondrial-lipid-binding domainPROSITEPS51847SMPcoord: 203..385
score: 14.780159

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS021147.1MS021147.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008289 lipid binding