MS021114 (gene) Bitter gourd (TR) v1

Overview


Name	MS021114
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Imidazole glycerol phosphate synthase hisHF, chloroplastic
Location	scaffold290: 1371307 .. 1371954 (+)
RNA-Seq Expression	MS021114
Synteny	MS021114

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTCTATGGCGATGACGACGACCACTGGAAATGTCCAAAACACCCTTCCAAGCGGCGGAGAACCGGAATCTGCCCTCTCTGCCTCCGCGACCGCCTCGTCTCTCTCTGCCCTGACTGCGCCACCGTCCGCCCCTGCGCCTGCTCCGCCACCGCCTCTTCCTCTTCCTCTTCTTCCTCCTCGTCTTTCTCCCGCGGATCCGTCGGGCGCGTCTCCAGTTTGATCGACAGCGAGCCGGCGTTCCGCCGCTCGCGCTCCGTCGCCGCGGCGGTTCCGTTTCTGCGACCGAGATCGAGATTCGTCGTCGATGTCGACGGCGACGGCGACGGGGACGACTGTTCCTCGGCGTCCGGTTACAGCGCCAGAGGAGCGCTGTCGTTCTGGTCGATGTTCTGGTCGCGGAAGAGCAAGAAGAGCCAGGACGGCGGAGGAATCGAGGCGGCGGCGGGAGTCGAGGAAGCGATGCGGCGGACGATGATGATCCGGTCGAGATCGGTTGCGGCGGTTTCTGACTCCAGCGGGAAGTTCGTGCGGCCGCCGCCGGCGAAGGGGCGGGGATGGTACTTTCCGAGTCCGATCAAAGTATTCCGGCAACCGAAGATTCCCAAGCCCGTTCTTACGGAACGGTCTCCGTTACACAGAGGT

mRNA sequence

ATGGCCTTCTATGGCGATGACGACGACCACTGGAAATGTCCAAAACACCCTTCCAAGCGGCGGAGAACCGGAATCTGCCCTCTCTGCCTCCGCGACCGCCTCGTCTCTCTCTGCCCTGACTGCGCCACCGTCCGCCCCTGCGCCTGCTCCGCCACCGCCTCTTCCTCTTCCTCTTCTTCCTCCTCGTCTTTCTCCCGCGGATCCGTCGGGCGCGTCTCCAGTTTGATCGACAGCGAGCCGGCGTTCCGCCGCTCGCGCTCCGTCGCCGCGGCGGTTCCGTTTCTGCGACCGAGATCGAGATTCGTCGTCGATGTCGACGGCGACGGCGACGGGGACGACTGTTCCTCGGCGTCCGGTTACAGCGCCAGAGGAGCGCTGTCGTTCTGGTCGATGTTCTGGTCGCGGAAGAGCAAGAAGAGCCAGGACGGCGGAGGAATCGAGGCGGCGGCGGGAGTCGAGGAAGCGATGCGGCGGACGATGATGATCCGGTCGAGATCGGTTGCGGCGGTTTCTGACTCCAGCGGGAAGTTCGTGCGGCCGCCGCCGGCGAAGGGGCGGGGATGGTACTTTCCGAGTCCGATCAAAGTATTCCGGCAACCGAAGATTCCCAAGCCCGTTCTTACGGAACGGTCTCCGTTACACAGAGGT

Coding sequence (CDS)

ATGGCCTTCTATGGCGATGACGACGACCACTGGAAATGTCCAAAACACCCTTCCAAGCGGCGGAGAACCGGAATCTGCCCTCTCTGCCTCCGCGACCGCCTCGTCTCTCTCTGCCCTGACTGCGCCACCGTCCGCCCCTGCGCCTGCTCCGCCACCGCCTCTTCCTCTTCCTCTTCTTCCTCCTCGTCTTTCTCCCGCGGATCCGTCGGGCGCGTCTCCAGTTTGATCGACAGCGAGCCGGCGTTCCGCCGCTCGCGCTCCGTCGCCGCGGCGGTTCCGTTTCTGCGACCGAGATCGAGATTCGTCGTCGATGTCGACGGCGACGGCGACGGGGACGACTGTTCCTCGGCGTCCGGTTACAGCGCCAGAGGAGCGCTGTCGTTCTGGTCGATGTTCTGGTCGCGGAAGAGCAAGAAGAGCCAGGACGGCGGAGGAATCGAGGCGGCGGCGGGAGTCGAGGAAGCGATGCGGCGGACGATGATGATCCGGTCGAGATCGGTTGCGGCGGTTTCTGACTCCAGCGGGAAGTTCGTGCGGCCGCCGCCGGCGAAGGGGCGGGGATGGTACTTTCCGAGTCCGATCAAAGTATTCCGGCAACCGAAGATTCCCAAGCCCGTTCTTACGGAACGGTCTCCGTTACACAGAGGT

Protein sequence

MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSATASSSSSSSSSSFSRGSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDGDGDDCSSASGYSARGALSFWSMFWSRKSKKSQDGGGIEAAAGVEEAMRRTMMIRSRSVAAVSDSSGKFVRPPPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG

Homology

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BLAST of MS021114 vs. NCBI nr
Match: XP_022145394.1 (uncharacterized protein LOC111014850 [Momordica charantia])

HSP 1 Score: 423.7 bits (1088), Expect = 9.5e-115
Identity = 214/216 (99.07%), Postives = 214/216 (99.07%), Query Frame = 0


Query: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSATASSSSSSS 60
           MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSATASSSSSSS
Sbjct: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSATASSSSSSS 60

Query: 61  SSSFSRGSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDGDGDDCSSASGY 120
           SSSFSRGSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDV GDGDGDDCSSASGY
Sbjct: 61  SSSFSRGSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVHGDGDGDDCSSASGY 120

Query: 121 SARGALSFWSMFWSRKSKKSQDGGGIEAAAGVEEAMRRTMMIRSRSVAAVSDSSGKFVRP 180
           SARGALSFWSMFWSRKSKKSQDGG IEAAAGVEEAMRRTMMIRSRSVAAVSDSSGKFVRP
Sbjct: 121 SARGALSFWSMFWSRKSKKSQDGGKIEAAAGVEEAMRRTMMIRSRSVAAVSDSSGKFVRP 180

Query: 181 PPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
           PPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG
Sbjct: 181 PPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 216

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BLAST of MS021114 vs. NCBI nr
Match: XP_023552753.1 (uncharacterized protein LOC111810294 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 281.2 bits (718), Expect = 7.6e-72
Identity = 170/239 (71.13%), Postives = 184/239 (76.99%), Query Frame = 0


Query: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSA--TASSSSS 60
           MAFYGD+DD WKCPKHPSKRRR GICPLCLRDRLV+LCPDCA VRPC C A  T +SSSS
Sbjct: 1   MAFYGDEDDVWKCPKHPSKRRRIGICPLCLRDRLVTLCPDCAHVRPCNCCATTTTTSSSS 60

Query: 61  SSSSSFSR---------GSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDG 120
           SSSSSFSR         GSVGR+S+LID EPAFRRSRS+ AA+PFLR RSRFV D     
Sbjct: 61  SSSSSFSRFSSADLASLGSVGRLSNLIDGEPAFRRSRSL-AAIPFLRSRSRFVAD----- 120

Query: 121 DGDDCSSASGYSARGALSFWSMFWSRKSKKSQDGGGIEAA------------AGVEEAMR 180
            GDDCSS SG SAR A SFWS+F S K+KKS+DGG IEAA            A VEEAMR
Sbjct: 121 SGDDCSS-SGNSAR-ASSFWSIFRS-KTKKSRDGGRIEAAVEFDFKRRAKEVAEVEEAMR 180

Query: 181 RTMMIRSRSVAAVSDSSGKFVRPPPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
           RT+MIRSRSV AVSDSSG+FVR  PAK + WYFPSPIK FRQ K+PK VLTERSPLHRG
Sbjct: 181 RTLMIRSRSV-AVSDSSGRFVR-TPAKAKAWYFPSPIKAFRQSKVPKAVLTERSPLHRG 228

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BLAST of MS021114 vs. NCBI nr
Match: XP_022923201.1 (uncharacterized protein LOC111430968 [Cucurbita moschata] >KAG7015751.1 hypothetical protein SDJN02_23389, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 280.4 bits (716), Expect = 1.3e-71
Identity = 169/239 (70.71%), Postives = 183/239 (76.57%), Query Frame = 0


Query: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSA--TASSSSS 60
           MAFYGD+DD WKCPKHPSKRRR GICPLCLRDRLV+LCPDCA VRPC C A  T +SSSS
Sbjct: 1   MAFYGDEDDVWKCPKHPSKRRRIGICPLCLRDRLVTLCPDCANVRPCNCCATTTTTSSSS 60

Query: 61  SSSSSFSR---------GSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDG 120
           SSSSSFSR         GSVGR+S+LID EPAFRRSRS+ AA+PFLR RSRFV D     
Sbjct: 61  SSSSSFSRFSSADLASLGSVGRLSNLIDGEPAFRRSRSL-AAIPFLRSRSRFVAD----- 120

Query: 121 DGDDCSSASGYSARGALSFWSMFWSRKSKKSQDGGGIEAA------------AGVEEAMR 180
            GDDCSS SG SAR A SFWS+F S K+KKS+DGG IEAA            A VEEAMR
Sbjct: 121 SGDDCSS-SGNSAR-ASSFWSIFRS-KTKKSRDGGRIEAAVEFDFKRRAKEVAEVEEAMR 180

Query: 181 RTMMIRSRSVAAVSDSSGKFVRPPPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
           RT+MIRSRSV AVSDSSG+FVR  P K + WYFPSPIK FRQ K+PK VLTERSPLHRG
Sbjct: 181 RTLMIRSRSV-AVSDSSGRFVR-TPVKAKAWYFPSPIKAFRQSKVPKAVLTERSPLHRG 228

[Close]

BLAST of MS021114 vs. NCBI nr
Match: XP_022965250.1 (uncharacterized protein LOC111465171 [Cucurbita maxima])

HSP 1 Score: 278.1 bits (710), Expect = 6.5e-71
Identity = 168/239 (70.29%), Postives = 182/239 (76.15%), Query Frame = 0


Query: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSA--TASSSSS 60
           MAFYGD+DD WKCPKHPSKRRR GICPLCLRDRL+SLCPDCA VRPC C A  T +SSSS
Sbjct: 1   MAFYGDEDDVWKCPKHPSKRRRIGICPLCLRDRLISLCPDCANVRPCNCCATTTTTSSSS 60

Query: 61  SSSSSFSR---------GSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDG 120
           SSSSSFSR         GSVGR+S+LID EPAFRRSRS+ AA+PFLR RSRFV D     
Sbjct: 61  SSSSSFSRFSSADLASLGSVGRLSNLIDGEPAFRRSRSL-AAIPFLRSRSRFVAD----- 120

Query: 121 DGDDCSSASGYSARGALSFWSMFWSRKSKKSQDGGGIEAA------------AGVEEAMR 180
            GDDCSS SG SAR A SFWS+F S K+KKS+DGG IEAA            A VEEAMR
Sbjct: 121 SGDDCSS-SGNSAR-ASSFWSIFRS-KTKKSRDGGRIEAAVEFDFKRRAKEVAEVEEAMR 180

Query: 181 RTMMIRSRSVAAVSDSSGKFVRPPPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
           RT+MIRSRSV AVSDSSG+FVR  P K + WYFPSPIK FRQ K+ K VLTERSPLHRG
Sbjct: 181 RTLMIRSRSV-AVSDSSGRFVR-TPVKAKAWYFPSPIKAFRQSKVSKAVLTERSPLHRG 228

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BLAST of MS021114 vs. NCBI nr
Match: XP_004148742.1 (uncharacterized protein LOC101206077 [Cucumis sativus] >KGN55746.1 hypothetical protein Csa_009559 [Cucumis sativus])

HSP 1 Score: 267.3 bits (682), Expect = 1.1e-67
Identity = 164/238 (68.91%), Postives = 179/238 (75.21%), Query Frame = 0


Query: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSA----TASSS 60
           MAFYGD+DD WKCPKHPSKRRR GICPLCLRDRLV+LCPDCA VRPC C A    T +SS
Sbjct: 1   MAFYGDEDDLWKCPKHPSKRRRIGICPLCLRDRLVTLCPDCANVRPCNCCATTNTTTTSS 60

Query: 61  SSSSSSSFSR------GSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDGD 120
           SSSSSSSFSR      GSVGR+S+LID EPAFRRSRS+ AA+PFL  RSRFV D      
Sbjct: 61  SSSSSSSFSRFSSADLGSVGRLSNLIDGEPAFRRSRSL-AAIPFL--RSRFVAD-----S 120

Query: 121 GDDCSSASGYSARGALSFWSMFWSRKSKKSQDGGGIEAA------------AGVEEAMRR 180
           GDDCSS SG SAR + SFWS+F S KSKK  DGG +EAA            A VEEAMRR
Sbjct: 121 GDDCSS-SGNSARTS-SFWSIFKS-KSKKRNDGGRMEAAVEIDFRRRAKEVAEVEEAMRR 180

Query: 181 TMMIRSRSVAAVSDSSGKFVRPPPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
            +MIRSRSV AV+DS G+ VR PP K + WYFPSPIK FRQ K+PKPVLTERSPLHRG
Sbjct: 181 KLMIRSRSV-AVADSGGRIVR-PPVKAKTWYFPSPIKAFRQSKLPKPVLTERSPLHRG 225

[Close]

BLAST of MS021114 vs. ExPASy TrEMBL
Match: A0A6J1CV41 (uncharacterized protein LOC111014850 OS=Momordica charantia OX=3673 GN=LOC111014850 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 4.6e-115
Identity = 214/216 (99.07%), Postives = 214/216 (99.07%), Query Frame = 0


Query: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSATASSSSSSS 60
           MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSATASSSSSSS
Sbjct: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSATASSSSSSS 60

Query: 61  SSSFSRGSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDGDGDDCSSASGY 120
           SSSFSRGSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDV GDGDGDDCSSASGY
Sbjct: 61  SSSFSRGSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVHGDGDGDDCSSASGY 120

Query: 121 SARGALSFWSMFWSRKSKKSQDGGGIEAAAGVEEAMRRTMMIRSRSVAAVSDSSGKFVRP 180
           SARGALSFWSMFWSRKSKKSQDGG IEAAAGVEEAMRRTMMIRSRSVAAVSDSSGKFVRP
Sbjct: 121 SARGALSFWSMFWSRKSKKSQDGGKIEAAAGVEEAMRRTMMIRSRSVAAVSDSSGKFVRP 180

Query: 181 PPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
           PPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG
Sbjct: 181 PPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 216

[Close]

BLAST of MS021114 vs. ExPASy TrEMBL
Match: A0A6J1E664 (uncharacterized protein LOC111430968 OS=Cucurbita moschata OX=3662 GN=LOC111430968 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 6.3e-72
Identity = 169/239 (70.71%), Postives = 183/239 (76.57%), Query Frame = 0


Query: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSA--TASSSSS 60
           MAFYGD+DD WKCPKHPSKRRR GICPLCLRDRLV+LCPDCA VRPC C A  T +SSSS
Sbjct: 1   MAFYGDEDDVWKCPKHPSKRRRIGICPLCLRDRLVTLCPDCANVRPCNCCATTTTTSSSS 60

Query: 61  SSSSSFSR---------GSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDG 120
           SSSSSFSR         GSVGR+S+LID EPAFRRSRS+ AA+PFLR RSRFV D     
Sbjct: 61  SSSSSFSRFSSADLASLGSVGRLSNLIDGEPAFRRSRSL-AAIPFLRSRSRFVAD----- 120

Query: 121 DGDDCSSASGYSARGALSFWSMFWSRKSKKSQDGGGIEAA------------AGVEEAMR 180
            GDDCSS SG SAR A SFWS+F S K+KKS+DGG IEAA            A VEEAMR
Sbjct: 121 SGDDCSS-SGNSAR-ASSFWSIFRS-KTKKSRDGGRIEAAVEFDFKRRAKEVAEVEEAMR 180

Query: 181 RTMMIRSRSVAAVSDSSGKFVRPPPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
           RT+MIRSRSV AVSDSSG+FVR  P K + WYFPSPIK FRQ K+PK VLTERSPLHRG
Sbjct: 181 RTLMIRSRSV-AVSDSSGRFVR-TPVKAKAWYFPSPIKAFRQSKVPKAVLTERSPLHRG 228

[Close]

BLAST of MS021114 vs. ExPASy TrEMBL
Match: A0A6J1HN71 (uncharacterized protein LOC111465171 OS=Cucurbita maxima OX=3661 GN=LOC111465171 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.1e-71
Identity = 168/239 (70.29%), Postives = 182/239 (76.15%), Query Frame = 0


Query: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSA--TASSSSS 60
           MAFYGD+DD WKCPKHPSKRRR GICPLCLRDRL+SLCPDCA VRPC C A  T +SSSS
Sbjct: 1   MAFYGDEDDVWKCPKHPSKRRRIGICPLCLRDRLISLCPDCANVRPCNCCATTTTTSSSS 60

Query: 61  SSSSSFSR---------GSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDG 120
           SSSSSFSR         GSVGR+S+LID EPAFRRSRS+ AA+PFLR RSRFV D     
Sbjct: 61  SSSSSFSRFSSADLASLGSVGRLSNLIDGEPAFRRSRSL-AAIPFLRSRSRFVAD----- 120

Query: 121 DGDDCSSASGYSARGALSFWSMFWSRKSKKSQDGGGIEAA------------AGVEEAMR 180
            GDDCSS SG SAR A SFWS+F S K+KKS+DGG IEAA            A VEEAMR
Sbjct: 121 SGDDCSS-SGNSAR-ASSFWSIFRS-KTKKSRDGGRIEAAVEFDFKRRAKEVAEVEEAMR 180

Query: 181 RTMMIRSRSVAAVSDSSGKFVRPPPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
           RT+MIRSRSV AVSDSSG+FVR  P K + WYFPSPIK FRQ K+ K VLTERSPLHRG
Sbjct: 181 RTLMIRSRSV-AVSDSSGRFVR-TPVKAKAWYFPSPIKAFRQSKVSKAVLTERSPLHRG 228

[Close]

BLAST of MS021114 vs. ExPASy TrEMBL
Match: A0A0A0L6G5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G009520 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 5.5e-68
Identity = 164/238 (68.91%), Postives = 179/238 (75.21%), Query Frame = 0


Query: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSA----TASSS 60
           MAFYGD+DD WKCPKHPSKRRR GICPLCLRDRLV+LCPDCA VRPC C A    T +SS
Sbjct: 1   MAFYGDEDDLWKCPKHPSKRRRIGICPLCLRDRLVTLCPDCANVRPCNCCATTNTTTTSS 60

Query: 61  SSSSSSSFSR------GSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDGD 120
           SSSSSSSFSR      GSVGR+S+LID EPAFRRSRS+ AA+PFL  RSRFV D      
Sbjct: 61  SSSSSSSFSRFSSADLGSVGRLSNLIDGEPAFRRSRSL-AAIPFL--RSRFVAD-----S 120

Query: 121 GDDCSSASGYSARGALSFWSMFWSRKSKKSQDGGGIEAA------------AGVEEAMRR 180
           GDDCSS SG SAR + SFWS+F S KSKK  DGG +EAA            A VEEAMRR
Sbjct: 121 GDDCSS-SGNSARTS-SFWSIFKS-KSKKRNDGGRMEAAVEIDFRRRAKEVAEVEEAMRR 180

Query: 181 TMMIRSRSVAAVSDSSGKFVRPPPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
            +MIRSRSV AV+DS G+ VR PP K + WYFPSPIK FRQ K+PKPVLTERSPLHRG
Sbjct: 181 KLMIRSRSV-AVADSGGRIVR-PPVKAKTWYFPSPIKAFRQSKLPKPVLTERSPLHRG 225

[Close]

BLAST of MS021114 vs. ExPASy TrEMBL
Match: A0A5A7UH78 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001530 PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 2.3e-66
Identity = 160/232 (68.97%), Postives = 174/232 (75.00%), Query Frame = 0


Query: 1   MAFYGDDDDHWKCPKHPSKRRRTGICPLCLRDRLVSLCPDCATVRPCACSA----TASSS 60
           MAFYGD+DD WKCPKHPSKRRR GICPLCLRDRLV+LCPDCA VRPC C A    T +SS
Sbjct: 1   MAFYGDEDDLWKCPKHPSKRRRIGICPLCLRDRLVTLCPDCANVRPCNCCATTNTTTTSS 60

Query: 61  SSSSSSSFSR------GSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDGD 120
           SSSSSSSFSR      GSVGR+S+LID EPAFRRSRS+ AA+PFL  RSRFV D      
Sbjct: 61  SSSSSSSFSRFSSADLGSVGRLSNLIDGEPAFRRSRSL-AAIPFL--RSRFVAD-----S 120

Query: 121 GDDCSSASGYSARGALSFWSMFWSRKSKKSQDGGGIE------AAAGVEEAMRRTMMIRS 180
            DDCSS+   SAR + SFWS+F S KSKK  DGG I+        A VEEAMRR +MIRS
Sbjct: 121 VDDCSSSGNNSARTS-SFWSIFRS-KSKKRNDGGEIDFRRRAKEVAEVEEAMRRKLMIRS 180

Query: 181 RSVAAVSDSSGKFVRPPPAKGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
           RSV AV+DS G+ VR PP K R WYFPSPIK FRQ KI KPVLTERSPLHRG
Sbjct: 181 RSV-AVADSGGRIVR-PPVKPRTWYFPSPIKAFRQTKISKPVLTERSPLHRG 220

[Close]

BLAST of MS021114 vs. TAIR 10
Match: AT1G32690.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 11 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G35200.1); Has 45 Blast hits to 45 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 45; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 123.2 bits (308), Expect = 2.5e-28
Identity = 98/232 (42.24%), Postives = 126/232 (54.31%), Query Frame = 0


Query: 6   DDDDHWKCPKHPSKR---RRTGICPLCLRDRLVSLCPDCATVRPCACSATA----SSSSS 65
           D+D   +C KH SKR      G+CP CL +RL SLCPDCA   PC+CS+ A    S SSS
Sbjct: 2   DEDGFSQCSKHFSKRGYFSGGGVCPFCLHERLSSLCPDCAHDLPCSCSSRAAVSFSPSSS 61

Query: 66  SSSSSFS------------RGSVGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVD 125
           SSSSSFS             GSVGRV+SLI+ EPAFRRS+S+  A+P ++P S  V+D  
Sbjct: 62  SSSSSFSIFAGDISFNISGVGSVGRVASLIECEPAFRRSKSM--AIP-IKPDS--VID-- 121

Query: 126 GDGDGDDCSSASGYSARGALSFWSMFWSRKSKKSQDGGGIEAAAGVEEAMRRTMMIRSRS 185
                   S      ++   SFW +F   +                    +  +M +SRS
Sbjct: 122 --------SGLDHGRSKKTSSFWRIFMGNRGD-----------------TKPAIMKKSRS 181

Query: 186 VAAVSDSSGKFVRPPPA--KGRGWYFPSPIKVFRQPKIPKPVLTERSPLHRG 217
           V AV+   G    P PA  KG+GW FPSPIKVFRQ ++ K +  +RSPL+RG
Sbjct: 182 V-AVAGEFGFSPVPVPATGKGKGWNFPSPIKVFRQSRVSKMIFQQRSPLYRG 200

[Close]

BLAST of MS021114 vs. TAIR 10
Match: AT2G35200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G32690.1); Has 36 Blast hits to 36 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 36; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 105.1 bits (261), Expect = 7.0e-23
Identity = 81/208 (38.94%), Postives = 112/208 (53.85%), Query Frame = 0


Query: 12  KCPKHPSK-RRRTGICPLCLRDRLVSLCPDCATVRPCACSATAS-SSSSSSSSSFSR-GS 71
           KC +H  +     G+CP CL +RL SLCPDCA   PC+C+  AS SS      SF+R GS
Sbjct: 22  KCSRHEGRFVCVAGVCPYCLHERLSSLCPDCAHDLPCSCTPRASVSSRGEGDVSFARIGS 81

Query: 72  VGRVSSLIDSEPAFRRSRSVAAAVPFLRPRSRFVVDVDGDGDGDDCSSASGYSARGALSF 131
           VGRV++LI+ EPAFRRS S+     F   +   +++ + D              RG    
Sbjct: 82  VGRVANLIECEPAFRRSTSITGPY-FWSAKPEPILETEKDLK----------PGRGRW-L 141

Query: 132 WSMFWSRKSKKSQDGGGIEAAAGVEEAMRRTMMIRSRSVAAVSDSSGKFVRPPPAKGRGW 191
           W +F   + ++++    I+AA          +M +S SVA     S     P  +KG GW
Sbjct: 142 WRLFRGNREEETK----IKAA---------KVMKKSNSVAGEVLFS---PTPVTSKGNGW 201

Query: 192 YFPSPIKVFRQPKIPKPVLTERSPLHRG 217
           YFPSPIKVFRQ ++ K +  +RSPL+RG
Sbjct: 202 YFPSPIKVFRQSRVSKMIFQQRSPLYRG 201

The following BLAST results are available for this feature:

BLAST of MS021114 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145394.1	9.5e-115	99.07	uncharacterized protein LOC111014850 [Momordica charantia]	[more]
XP_023552753.1	7.6e-72	71.13	uncharacterized protein LOC111810294 [Cucurbita pepo subsp. pepo]	[more]
XP_022923201.1	1.3e-71	70.71	uncharacterized protein LOC111430968 [Cucurbita moschata] >KAG7015751.1 hypothet...	[more]
XP_022965250.1	6.5e-71	70.29	uncharacterized protein LOC111465171 [Cucurbita maxima]	[more]
XP_004148742.1	1.1e-67	68.91	uncharacterized protein LOC101206077 [Cucumis sativus] >KGN55746.1 hypothetical ...	[more]

BLAST of MS021114 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS021114 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CV41	4.6e-115	99.07	uncharacterized protein LOC111014850 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A6J1E664	6.3e-72	70.71	uncharacterized protein LOC111430968 OS=Cucurbita moschata OX=3662 GN=LOC1114309...	[more]
A0A6J1HN71	3.1e-71	70.29	uncharacterized protein LOC111465171 OS=Cucurbita maxima OX=3661 GN=LOC111465171...	[more]
A0A0A0L6G5	5.5e-68	68.91	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G009520 PE=4 SV=1	[more]
A0A5A7UH78	2.3e-66	68.97	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of MS021114 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32690.1	2.5e-28	42.24	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT2G35200.1	7.0e-23	38.94	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008004	Protein OCTOPUS-like	PFAM	PF05340	DUF740	coord: 12..91 e-value: 7.4E-5 score: 21.6
None	No IPR available	PANTHER	PTHR34197	OS04G0591300 PROTEIN	coord: 1..216
None	No IPR available	PANTHER	PTHR34197:SF2	OS04G0591300 PROTEIN	coord: 1..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021114.1	MS021114.1	mRNA