MS021081 (gene) Bitter gourd (TR) v1

Overview
NameMS021081
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPhosphomevalonate kinase
Locationscaffold290: 1081697 .. 1086982 (-)
RNA-Seq ExpressionMS021081
SyntenyMS021081
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTACAGAGTTGCTTCAGCTCCTGGGAAAGTACTATTGACGGGAGGATACCTGATTTTGGAGAGGCCAAATGCTGGTTTGGTACTCAGTACAAATGCTCGGTTTTATGCAATTGTGAAACCACTTCACGAGCATGCTGAGCCAGAAAGTGGGGGTGGGGTATGTAACCTATCATGATTCTGCAAGTGTTAATAGTTTGGGTCATAACAATTTTATATCTTTTTTTTTTCAGTTTTTGAAATTTAAGTTTACAAACACTAATTTCACCATTGTATTTCTTTGCATTGTTATCTACTTTTCAAAATCTAAGCCAATTTTGAAAAACAGTAGGTTTTAGAAACTTATTTTTGTTTTTGAAATTTGGCTAGGAATTTGGATGTGCATGATGGAATGGTGACATAAATATGCACAAATATCAAAGTAATTGAGAGGAAGTATGTGTAATTTTAAGAACAGAAAACACTTGACGAAAATGTTATCAATTGGGACATTAACAAGTTGTTTAAATTCTGCTGTTTATACGTCCGAAGTGACTTGTGCTATGATTATTTGTTTACTGATAGGGCATTATTTTCCTTCTAAAACTGCTTTTAGAGACTCGAGTTTTGTACTCTTTCCCCTAAAATAATTGTTTTGATTACTGTCATGCTGAGTACCATTCGTATCGCAATACATTTCAACAATCTTTTTTCTTTTCTCAGGAATGGGTAGATGTGAAATTGACCTCACCCCAGCTCTCTCGAGAAAGTCTATATAGATTGTCGCTAGAGAACTTTACGGTTCAGTGTGTCTCCTCAAGGTAAGTCATTTTTACCTCTATGATGTATGCAAACTTATTTTTATTCAAGAAGTAAATTTGAGTTTTCACTGTATATTAGATTTTCTATACGACCAATTAGGTTAATACAGGTACTGTGACTGTCCATAATTTCAGTGAGTCGAGGAATCCTTTTGTAGAACATGCAGTACAATACTCCTTAGCTGCTACACATGGAATATTTGATAATAACAAGAAAGATTCACTGCACAGATTGCTACTGCAAGGTAAAATCAGTATGATTTTCTTCTCAGTCGTCTACAACCATTAATGCTACAAACTAAATTTCCAACGGGTTAGTTTTGCAGGTCTGGATATCACAATTTTAGGTTGCAATGATTTCTACTCTTATCGGAATCAGGTGCTTGATATGTTTCGAAATCCATCAACGATTCATTCTTATTTCTGAACATCTATTCTACAATACAACACAAATATCTCTTTTTGTTTGCGACTCTATTCGCTTTATAAGGAGTGTAAAGAAAAATACTTAGTTGTTTACTAATCAGTACCCATCTTTTGCTGACTGCATCAAATTTTTTACTAATAGAGAAATACGGAGAGACATTTTTTTCTGTTTTTAAAAAAGTAAACGACTGTCAAATTTTTATTGGTAGTTAGCATGAAGATAGATGCTAACTAGTAAGTACCTAAGTATTATTTTGGTATAAATATTTGTTTAGAACCGAGGAAACACTATCTCATAAAGTCAGTGAATACCTTGTTTTTCCTAAATAACAATTAATTATTTCCTTCAAAATGTGTTTCTAGGACATTGAGGTTCCATTTTTGATGTTAATGAGCTGTTACCATTTCATAGATTGAAGCACGTGGACTTCCTTTGACACCAGAATCACTGATTTCTTTTCCACCATTTGCATCAATTACCTTCAATGCTGAAGAATCAAATAGTGAAAATTGCAAGCCCGAAGTTGCAAAAACAGGATTGGGATCATCTGCTGCCATGACAACTTCTGTTGTTGCTGCTCTACTTAATTATTTTGGTGTCGTAAATCTTTCCTCATCAAAAGGTCAAGACAAGGAAAATAAAATTAAGGATCTTGATATGGTGCATGTAATAGCCCAAACTGCTCACTGCATTGCACAGGGGAAAGTTGGAAGTGGATTTGATGTCAGTTCTGCAGTCTATGGTAGCCAACGTTATGTTCGTTTTTCACCTGATGTGCTAGCTCCCGCTCAGGTATATAGCCATCTGCTACTCACTATTTAGCTCTCATTTCTCCGTTTCTTTACAATCTATATTCTGAGTGGCTAGTCACCGTCTGCTTACAGACTGCACCATCAAACCGAAACACTCAAATCGCATTTTATTATTCATGCACCAATACTTTTTCCATTTCACAAAATTCTGCATACTCCTTTTCCTAAGTTCTTAACACTTGAAATTTGCTTGCAAGGTGCTTTACATTAACCATCAGTAGCTGTGCTATTTTAGTGGCAGCATGGCTGATTTATGTTTTAAATGGAATGAAGTTAGAGCTTTTTTCATCTGACCTTTTCACTTGTTTTTATCTGGGCATGGAGTTGACGGTGACATGATTGTCATACCTTTTCCTTTGATATTGAAATTCAACTCTTTTGGCTATATATTATTCAGTATTTGTTGATAACTTTGATCGTTTTTAAACAATATGATATTCCTCCTGTAGTGCTCGAAGGGACTAATCTTAATATCCAGTACCTGTCTGTGTACAATGTTGAAATGTTGCTTTTTCTTTCACGCTGAAGGTTGAGTTTGTGAAAAAATTTTCCAAGGGTAAAAAATGCTTTTCACTAATTAAAAACAATTTTCAAAATTCTTAATTAGATAATCGATGATTTTGAAAATTAAAAAACACTTCAAAGAAGCTTTTAGAAAAATACTTAATTAGTTTCTTTCGAGAGTGCTCATAAAAGAAGTGCTTAACTTAAAAAAAAAAACACTTTTCTCAAAATCCATCCCAAACTCACTGTAAGTTTCGGTGGAGAATATCTCATCATAAGGTCCTCTTATCTTGAAGTAGGCCTTGGTGCAAATTCTGAATTTCGATTACAAATTTTCTGCTTTTGAGAATTCCAAAGACCTTGAATGTCTTCCTGAACTTGTATTGTCTTTTATTCATTTTTATTATTTCAGGCGGCAGTTAAAGGACCACTAGAAGAAGTCATCGTCAATATCTTAAAAGGAAACTGGGACCATGAGAGGACTGAGTTTTCCTTGCCTCCATTAATGAATCTTGTGAGAAAGTTTGGTATTCCCCCTTGTATTTATTTAAATAACTATTTAGATATTTGCCAATATTTCCTAAATATTTAGGTGTATTTTATGGCTTCTAGTGCTAGGTTCTTTCTTTATGTGGCACAGTTTACTCTAAAGATCTTTTGTGGATATAGTATCTTTACATATATTCACTTCAAAATGAAACTTTTCTATATCAAAGCAGCTTCGTTCTCAGGTTATGTAGTATGAAAATAAATAACCCCATCTGACAACCATTTCGTTTTTTGTTTTTTGTTTTTAAAAATTATGTTTGATTTTTACCATTTCTTTGGTATGTTTTTCACCTTCCTTAAAAACATATTTCAATTATTATCCAAATTTAGAAAACAAAAATAAGATTTTAAAAACAACCTTTTTTAGTTTTCAAAATTTAGCATAGATTTTGAAAATATTTTTAAAAATTAAATATTAAGATAAATAAGCATATAGGTGGAAGTAGTGTTTCTAAGTTTAATTTTTAAAAACAGAACACAAAAAACCAAATAGTTATCAGACGGTCTACTTTATTCAATCACTGAGACTATCTTTCTTCAATGTTCTTGTTTTTTTTGTCAGTTATTAGGGGAGCCAGGAGCAGGAGGGTCATCCACACCATCTATGGTAGGTGCCGTTAAAAAGTGGCAAAAGTCTGACCCTCAGAAATCTCAAGAAACATGGAATAAGTTGTCCGAAGCGAATCTTGATCTTGAAAACCAACTAAACTTACTAAGAAAGCTTGCAGCAGAACACTGGGATGTGTACAAATGCATTATAAGCAAATGCAGCACACTTAGATCAAAGAAGGTACTTCCCGAGCTCAACTAAGACATGTTTGGAAGTGATTTTGTCACACTTGCCATTGTCTAAACTCACTCATATTATTGTCAAACATTACTACTTTAAGACGGGGTGATTTTGACAATGATATACCGTAATTTAAAAAAATGGCTAAAGTGATATTTTTGTCCCTGTGTGATGGTTTACTACATTTTATCCATCTTTATGTTAATTGACCTTAAATTGTTACAAATTGGTATTAGTGGATGGAACAAGCCACTGAACCAAGCCAACAAACCATTGTTAAAGCATTGTTGGGAGCAAGAGATGCTATGCTCGAGATACGAAGTAATATGCGGCAAATGGGCGAAGCTGCAGGTGTTCCAGTAAGTCCTTTTTTTCTTTATTTTATCTCCAAAGCGACAAATAGGAAAGTGTGTGTTTAGCTAGCTAAGTCCCTCTTTTTGTTCTAAAAAAAAAAAGTAACTCCCTTTTTTTCTTTATTTTATTTTATTAAGTGATTGTTTCTTATAAAATAAAATATAATAGTGTAACTAAGTGTGTGTTTTGGGATGATTTTTAAATGAAAATACTCTTTTTATAAAGTTTTTTTTTAATTACTAATTTAGTGCAGGTTTAGTATAATTTCCTAGGTAATTTTAGATGACTAAAAGTACTTTTTGAAAAAGCTACATATTTAGTAGTAAATTTAAAAAAGAAACATTGTAAAAGAATCAGATTGGTTTAAAAATACTTTTTGAAGAAACAGTTGAGATAATTATTCAAAAAGTGACTTCCAAGCGATCTGACACCCTTGTAGTTTAAACACTCGAGAGATGATTGTTAAGAAAATGCTTAGACTGGAAGGTATGAAACTCATGAAAAGAGTGATTCGTTACAGTTATGATAGTATATATATTGTTGGAACAATTACATTAATAGTTATCTTTATGCTCAAATCTATGTTCATAGTTGCATTTTAAGTGGTAGTTAGGGGTACGACAGCTACAATTTATGATGAAAGTAGAAATAGTAGTGAAAGTGAAAGTTCTAATAAGAACTAGCACGAATGAGAACCGAAAATTCTAATTATCTAGATGGAACATTTTGGGCTGAACAGTACAAAATTTAGAGTACTGAGTTCTGTTGTTGGATATAATATATTAAATATATATATATATATATGCAGATTGAACCAAAGTCCCAAAGTGAGGTTTTGGATGCAACCATGGAATTGGAAGGAGTGTTGTTGGGTGGAGTTCCAGGAGCAGGTGGGTTTGATGCTGTATTTGCTATTACATTGGGTGATTCTTGTAGCAGCCATGTGATCAACTCATGGACTTCACTCAATGTTCTTCCCCTCCTACTTAAAGAAGATCCCAATGGAGTTGCTCTACAAACTGCTGATCCTAGAACTAACAATATTGCATCT

mRNA sequence

TTCTACAGAGTTGCTTCAGCTCCTGGGAAAGTACTATTGACGGGAGGATACCTGATTTTGGAGAGGCCAAATGCTGGTTTGGTACTCAGTACAAATGCTCGGTTTTATGCAATTGTGAAACCACTTCACGAGCATGCTGAGCCAGAAAGTGGGGGTGGGGAATGGGTAGATGTGAAATTGACCTCACCCCAGCTCTCTCGAGAAAGTCTATATAGATTGTCGCTAGAGAACTTTACGGTTCAGTGTGTCTCCTCAAGTGAGTCGAGGAATCCTTTTGTAGAACATGCAGTACAATACTCCTTAGCTGCTACACATGGAATATTTGATAATAACAAGAAAGATTCACTGCACAGATTGCTACTGCAAGGTCTGGATATCACAATTTTAGGTTGCAATGATTTCTACTCTTATCGGAATCAGATTGAAGCACGTGGACTTCCTTTGACACCAGAATCACTGATTTCTTTTCCACCATTTGCATCAATTACCTTCAATGCTGAAGAATCAAATAGTGAAAATTGCAAGCCCGAAGTTGCAAAAACAGGATTGGGATCATCTGCTGCCATGACAACTTCTGTTGTTGCTGCTCTACTTAATTATTTTGGTGTCGTAAATCTTTCCTCATCAAAAGGTCAAGACAAGGAAAATAAAATTAAGGATCTTGATATGGTGCATGTAATAGCCCAAACTGCTCACTGCATTGCACAGGGGAAAGTTGGAAGTGGATTTGATGTCAGTTCTGCAGTCTATGGTAGCCAACGTTATGTTCGTTTTTCACCTGATGTGCTAGCTCCCGCTCAGGCGGCAGTTAAAGGACCACTAGAAGAAGTCATCGTCAATATCTTAAAAGGAAACTGGGACCATGAGAGGACTGAGTTTTCCTTGCCTCCATTAATGAATCTTTTATTAGGGGAGCCAGGAGCAGGAGGGTCATCCACACCATCTATGGTAGGTGCCGTTAAAAAGTGGCAAAAGTCTGACCCTCAGAAATCTCAAGAAACATGGAATAAGTTGTCCGAAGCGAATCTTGATCTTGAAAACCAACTAAACTTACTAAGAAAGCTTGCAGCAGAACACTGGGATGTGTACAAATGCATTATAAGCAAATGCAGCACACTTAGATCAAAGAAGTGGATGGAACAAGCCACTGAACCAAGCCAACAAACCATTGTTAAAGCATTGTTGGGAGCAAGAGATGCTATGCTCGAGATACGAAGTAATATGCGGCAAATGGGCGAAGCTGCAGGTGTTCCAATTGAACCAAAGTCCCAAAGTGAGGTTTTGGATGCAACCATGGAATTGGAAGGAGTGTTGTTGGGTGGAGTTCCAGGAGCAGGTGGGTTTGATGCTGTATTTGCTATTACATTGGGTGATTCTTGTAGCAGCCATGTGATCAACTCATGGACTTCACTCAATGTTCTTCCCCTCCTACTTAAAGAAGATCCCAATGGAGTTGCTCTACAAACTGCTGATCCTAGAACTAACAATATTGCATCT

Coding sequence (CDS)

TTCTACAGAGTTGCTTCAGCTCCTGGGAAAGTACTATTGACGGGAGGATACCTGATTTTGGAGAGGCCAAATGCTGGTTTGGTACTCAGTACAAATGCTCGGTTTTATGCAATTGTGAAACCACTTCACGAGCATGCTGAGCCAGAAAGTGGGGGTGGGGAATGGGTAGATGTGAAATTGACCTCACCCCAGCTCTCTCGAGAAAGTCTATATAGATTGTCGCTAGAGAACTTTACGGTTCAGTGTGTCTCCTCAAGTGAGTCGAGGAATCCTTTTGTAGAACATGCAGTACAATACTCCTTAGCTGCTACACATGGAATATTTGATAATAACAAGAAAGATTCACTGCACAGATTGCTACTGCAAGGTCTGGATATCACAATTTTAGGTTGCAATGATTTCTACTCTTATCGGAATCAGATTGAAGCACGTGGACTTCCTTTGACACCAGAATCACTGATTTCTTTTCCACCATTTGCATCAATTACCTTCAATGCTGAAGAATCAAATAGTGAAAATTGCAAGCCCGAAGTTGCAAAAACAGGATTGGGATCATCTGCTGCCATGACAACTTCTGTTGTTGCTGCTCTACTTAATTATTTTGGTGTCGTAAATCTTTCCTCATCAAAAGGTCAAGACAAGGAAAATAAAATTAAGGATCTTGATATGGTGCATGTAATAGCCCAAACTGCTCACTGCATTGCACAGGGGAAAGTTGGAAGTGGATTTGATGTCAGTTCTGCAGTCTATGGTAGCCAACGTTATGTTCGTTTTTCACCTGATGTGCTAGCTCCCGCTCAGGCGGCAGTTAAAGGACCACTAGAAGAAGTCATCGTCAATATCTTAAAAGGAAACTGGGACCATGAGAGGACTGAGTTTTCCTTGCCTCCATTAATGAATCTTTTATTAGGGGAGCCAGGAGCAGGAGGGTCATCCACACCATCTATGGTAGGTGCCGTTAAAAAGTGGCAAAAGTCTGACCCTCAGAAATCTCAAGAAACATGGAATAAGTTGTCCGAAGCGAATCTTGATCTTGAAAACCAACTAAACTTACTAAGAAAGCTTGCAGCAGAACACTGGGATGTGTACAAATGCATTATAAGCAAATGCAGCACACTTAGATCAAAGAAGTGGATGGAACAAGCCACTGAACCAAGCCAACAAACCATTGTTAAAGCATTGTTGGGAGCAAGAGATGCTATGCTCGAGATACGAAGTAATATGCGGCAAATGGGCGAAGCTGCAGGTGTTCCAATTGAACCAAAGTCCCAAAGTGAGGTTTTGGATGCAACCATGGAATTGGAAGGAGTGTTGTTGGGTGGAGTTCCAGGAGCAGGTGGGTTTGATGCTGTATTTGCTATTACATTGGGTGATTCTTGTAGCAGCCATGTGATCAACTCATGGACTTCACTCAATGTTCTTCCCCTCCTACTTAAAGAAGATCCCAATGGAGTTGCTCTACAAACTGCTGATCCTAGAACTAACAATATTGCATCT

Protein sequence

FYRVASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSPQLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQGLDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGLGSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGFDVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLLGEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVYKCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKSQSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPNGVALQTADPRTNNIAS
Homology
BLAST of MS021081 vs. NCBI nr
Match: XP_022145447.1 (phosphomevalonate kinase, peroxisomal-like [Momordica charantia])

HSP 1 Score: 980.7 bits (2534), Expect = 4.7e-282
Identity = 494/496 (99.60%), Postives = 496/496 (100.00%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP
Sbjct: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG
Sbjct: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASI+FNAEESNSENCKPEVAKTGL
Sbjct: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASISFNAEESNSENCKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF
Sbjct: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303
           DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL
Sbjct: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303

Query: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363
           GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY
Sbjct: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363

Query: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423
           KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS
Sbjct: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423

Query: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPN 483
           QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDP+
Sbjct: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPD 483

Query: 484 GVALQTADPRTNNIAS 500
           GVALQTADPRTNNIAS
Sbjct: 484 GVALQTADPRTNNIAS 499

BLAST of MS021081 vs. NCBI nr
Match: AEM42973.1 (phosphomevalonate kinase [Siraitia grosvenorii])

HSP 1 Score: 889.8 bits (2298), Expect = 1.1e-254
Identity = 448/495 (90.51%), Postives = 467/495 (94.34%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEH EPESGGG+WVDVKLTSP
Sbjct: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHTEPESGGGKWVDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QL RESLY+LS ENFT+QC+SS E RNPFV+HAVQYS+AA HGIFD NKKDSLHRLLLQG
Sbjct: 64  QLCRESLYKLSPENFTIQCISSIELRNPFVKHAVQYSVAAAHGIFDKNKKDSLHRLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL
Sbjct: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTT+VVAALLNY GVVNLSS +GQDKEN IKDLDMVHVIAQTAHCIAQGKVGSGF
Sbjct: 184 GSSAAMTTAVVAALLNYLGVVNLSSPRGQDKENDIKDLDMVHVIAQTAHCIAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303
           DV SAVYGSQRYVRFSP+VLA AQ AVKGPLEEVIVNILKG WDHERTEFSLPPLMNLLL
Sbjct: 244 DVCSAVYGSQRYVRFSPEVLATAQVAVKGPLEEVIVNILKGKWDHERTEFSLPPLMNLLL 303

Query: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363
           GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANL LE QLNLLRKLAAEHWDVY
Sbjct: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLGLEKQLNLLRKLAAEHWDVY 363

Query: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423
           KCIIS CS L SKKWMEQATE SQQ IVKALLGARDAMLEIR++MRQMGEAAGVPIEPKS
Sbjct: 364 KCIISGCSILGSKKWMEQATEQSQQAIVKALLGARDAMLEIRNHMRQMGEAAGVPIEPKS 423

Query: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPN 483
           Q+++LDATMELEGVLL GVPGAGGFDAVFAITLGDS S HVI SWTSLNVL LL++EDPN
Sbjct: 424 QTQLLDATMELEGVLLAGVPGAGGFDAVFAITLGDS-SRHVIESWTSLNVLALLVREDPN 483

Query: 484 GVALQTADPRTNNIA 499
           GVAL++ DPR+ NIA
Sbjct: 484 GVALESGDPRSKNIA 497

BLAST of MS021081 vs. NCBI nr
Match: XP_022951530.1 (phosphomevalonate kinase, peroxisomal-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 863.6 bits (2230), Expect = 8.3e-247
Identity = 435/495 (87.88%), Postives = 462/495 (93.33%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           V SAPGKVLLTGGYLILERPN GLVLSTNARFYAIVKPLHE  E ESG GEWVDVKLTSP
Sbjct: 4   VTSAPGKVLLTGGYLILERPNPGLVLSTNARFYAIVKPLHEQTEAESGSGEWVDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QLSRESLY+LSL++FT+QC+S SES+N FVEHA+QYSLA  HGI D NKKDSLHRLLLQG
Sbjct: 64  QLSRESLYKLSLKSFTIQCISLSESKNLFVEHALQYSLAVAHGILDKNKKDSLHRLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILGCNDFYSYRNQIEARGLPLTPESL+SF PFASITFNAEES+SENCKPEVAKTGL
Sbjct: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLLSFTPFASITFNAEESSSENCKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTT+VVAALLNY GVVNLSSS+GQDKEN IKDLD+VHVIAQTAHCIAQGKVGSGF
Sbjct: 184 GSSAAMTTAVVAALLNYLGVVNLSSSRGQDKENDIKDLDIVHVIAQTAHCIAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303
           DVSSAVYGSQRYVRFSP+VL  AQAAVK PLEEVIV+ILKG WDHERTEFSLPPLMNLLL
Sbjct: 244 DVSSAVYGSQRYVRFSPNVLTTAQAAVKEPLEEVIVDILKGKWDHERTEFSLPPLMNLLL 303

Query: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363
           GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLE QLNLLRK+AAE WDVY
Sbjct: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLERQLNLLRKVAAEQWDVY 363

Query: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423
           KCI+SKCS L SKKWMEQATEP+QQ IVKALLGARDAMLEIR++MRQMGEAAG+PIEPKS
Sbjct: 364 KCILSKCSMLGSKKWMEQATEPNQQGIVKALLGARDAMLEIRNHMRQMGEAAGIPIEPKS 423

Query: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPN 483
           Q+E+LD TMELEGVLL GVPGAGGFDAVFAITLGDS SSHVI SWTSLNVL LL++EDPN
Sbjct: 424 QTELLDRTMELEGVLLAGVPGAGGFDAVFAITLGDS-SSHVIKSWTSLNVLALLVREDPN 483

Query: 484 GVALQTADPRTNNIA 499
           GVAL+  DPR+  +A
Sbjct: 484 GVALERGDPRSKCMA 497

BLAST of MS021081 vs. NCBI nr
Match: KAG6596708.1 (Phosphomevalonate kinase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028244.1 Phosphomevalonate kinase, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 863.2 bits (2229), Expect = 1.1e-246
Identity = 434/495 (87.68%), Postives = 460/495 (92.93%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           V SAPGKVLLTGGYLILERPN GLVLSTNARFYAIVKPLHE  E ESG GEWVDVKLTSP
Sbjct: 4   VTSAPGKVLLTGGYLILERPNPGLVLSTNARFYAIVKPLHEQTEAESGSGEWVDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QLSRESLY+LSL+ FT+QC+  SES+N FVEHA+QYSLA  HGI D NKKDSLHRLLLQG
Sbjct: 64  QLSRESLYKLSLKRFTIQCIGLSESKNLFVEHALQYSLAVAHGILDKNKKDSLHRLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILGCNDFYSYRNQIEARGLPLTPESL+SF PFASITFNAEES+SENCKPEVAKTGL
Sbjct: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLLSFTPFASITFNAEESSSENCKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTT+VVAALLNY GVVNLSSS+GQDKEN IKDLD+VHVIAQTAHCIAQGKVGSGF
Sbjct: 184 GSSAAMTTAVVAALLNYLGVVNLSSSRGQDKENDIKDLDIVHVIAQTAHCIAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303
           DVSSAVYGSQRYVRFSPDVL  AQAAVK PLEEVIV+ILKG WDHERTEFSLPPLMNLLL
Sbjct: 244 DVSSAVYGSQRYVRFSPDVLTTAQAAVKEPLEEVIVDILKGKWDHERTEFSLPPLMNLLL 303

Query: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363
           GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLE QLNLLRK+AAE WDVY
Sbjct: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLERQLNLLRKVAAEQWDVY 363

Query: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423
           KCI+SKCS L SKKWMEQATEP+QQ IVKALLGARDAMLEIR++MRQMGEAAG+PIEPKS
Sbjct: 364 KCILSKCSMLGSKKWMEQATEPNQQAIVKALLGARDAMLEIRNHMRQMGEAAGIPIEPKS 423

Query: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPN 483
           Q+E+LD TMELEGVL+ GVPGAGGFDAVFAITLGDS SSHVI SWTSLNVL LL++EDPN
Sbjct: 424 QTELLDRTMELEGVLVAGVPGAGGFDAVFAITLGDS-SSHVIKSWTSLNVLALLVREDPN 483

Query: 484 GVALQTADPRTNNIA 499
           GVAL+  DPR+  +A
Sbjct: 484 GVALERGDPRSKCMA 497

BLAST of MS021081 vs. NCBI nr
Match: XP_023540458.1 (phosphomevalonate kinase, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 861.7 bits (2225), Expect = 3.2e-246
Identity = 432/495 (87.27%), Postives = 462/495 (93.33%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           V SAPGKVLLTGGYLILERPN GLVLSTNARFYAIVKPLHE  E E+G GEWVDVKLTSP
Sbjct: 4   VTSAPGKVLLTGGYLILERPNPGLVLSTNARFYAIVKPLHEKTEAENGSGEWVDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QLSRESLY+LSL++FT+QC+  SES+N FVEHA+QYSLA  HGI + NKKDSLHRLLLQG
Sbjct: 64  QLSRESLYKLSLKSFTIQCIGLSESKNLFVEHALQYSLAVAHGILEKNKKDSLHRLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILGCNDFYSYRNQIEARGLPLTPESL+SF PFASITFNAEES+SENCKPEVAKTGL
Sbjct: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLLSFTPFASITFNAEESSSENCKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTT+VVAALLNY GVVNLSS++GQDKEN IKDLD+VHVIAQTAHCIAQGKVGSGF
Sbjct: 184 GSSAAMTTAVVAALLNYLGVVNLSSTRGQDKENDIKDLDIVHVIAQTAHCIAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303
           DVSSAVYGSQRYVRFSPDVL  AQAAVK PLEEVIV+ILKG WDHERTEFSLPPLMNLLL
Sbjct: 244 DVSSAVYGSQRYVRFSPDVLTTAQAAVKEPLEEVIVDILKGKWDHERTEFSLPPLMNLLL 303

Query: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363
           GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLE QLNLLRK+AAE WDVY
Sbjct: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLERQLNLLRKVAAEQWDVY 363

Query: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423
           KCIISKCS L SKKWMEQATEP+QQ IVKALLGARDAMLEIR++MRQMGEAAG+PIEPKS
Sbjct: 364 KCIISKCSMLGSKKWMEQATEPNQQAIVKALLGARDAMLEIRNHMRQMGEAAGIPIEPKS 423

Query: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPN 483
           Q+E+LD TMELEGVL+ GVPGAGGFDAVFAITLGDS SSHVI SWTSLNVL LL++EDPN
Sbjct: 424 QTELLDRTMELEGVLVAGVPGAGGFDAVFAITLGDS-SSHVIKSWTSLNVLALLVREDPN 483

Query: 484 GVALQTADPRTNNIA 499
           GVAL+  DPR+ ++A
Sbjct: 484 GVALECGDPRSKSMA 497

BLAST of MS021081 vs. ExPASy Swiss-Prot
Match: Q9C6T1 (Phosphomevalonate kinase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=PMK PE=1 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 4.5e-195
Identity = 348/497 (70.02%), Postives = 411/497 (82.70%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           VASAPGKVL+TGGYL+LE+PNAGLVLSTNARFYAIVKP++E  +PES   +W DVKLTSP
Sbjct: 4   VASAPGKVLMTGGYLVLEKPNAGLVLSTNARFYAIVKPINEEVKPESWAWKWTDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QLSRES+Y+LSL + T+Q VS+S+SRNPFVEHA+QY++AA H +     K+SLH+LLLQG
Sbjct: 64  QLSRESMYKLSLNHLTLQSVSASDSRNPFVEHAIQYAIAAAH-LATEKDKESLHKLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILG NDFYSYRNQIE+ GLPLTPESL +  PFASITFNA ESN  N KPEVAKTGL
Sbjct: 124 LDITILGSNDFYSYRNQIESAGLPLTPESLGTLAPFASITFNAAESNGANSKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTT+VVAALL+Y GVV+LS    + K     DLD++H+IAQT+HC+AQGKVGSGF
Sbjct: 184 GSSAAMTTAVVAALLHYLGVVDLSDPCKEGKFG-CSDLDVIHMIAQTSHCLAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKG-PLEEVIVNILKGNWDHERTEFSLPPLMNLL 303
           DVS AVYGSQRYVRFSP+VL+ AQ AV G PL EVI  ILKG WD++RTEFSLPPLMNL 
Sbjct: 244 DVSCAVYGSQRYVRFSPEVLSFAQVAVTGLPLNEVIGTILKGKWDNKRTEFSLPPLMNLF 303

Query: 304 LGEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDV 363
           LGEPG+GGSSTPSMVGAVKKWQ SDP+K++E W  LS+ANL+LE +LN L KLA +HWDV
Sbjct: 304 LGEPGSGGSSTPSMVGAVKKWQMSDPEKARENWQNLSDANLELETKLNDLSKLAKDHWDV 363

Query: 364 YKCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPK 423
           Y  +I  CS L S+KW+  ATEP  + I+K LL AR+AML IR  MRQMGEAA VPIEP+
Sbjct: 364 YLRVIKSCSVLTSEKWVLHATEPINEAIIKELLEAREAMLRIRILMRQMGEAASVPIEPE 423

Query: 424 SQSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDP 483
           SQ+++LD+TM  EGVLL GVPGAGGFDA+FAITLGDS  + +  +W+S NVL LL++EDP
Sbjct: 424 SQTQLLDSTMSAEGVLLAGVPGAGGFDAIFAITLGDS-GTKLTQAWSSHNVLALLVREDP 483

Query: 484 NGVALQTADPRTNNIAS 500
           +GV L++ DPRT  I S
Sbjct: 484 HGVCLESGDPRTTCITS 497

BLAST of MS021081 vs. ExPASy Swiss-Prot
Match: Q9UT88 (Probable phosphomevalonate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=erg8 PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.4e-42
Identity = 157/499 (31.46%), Postives = 237/499 (47.49%), Query Frame = 0

Query: 6   SAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHA------EPESGGGEWV-DV 65
           SAPGKVL+ GGY++L+   +GLV+   A+ YA    L +         P+    EW+ ++
Sbjct: 6   SAPGKVLIAGGYIVLDPQYSGLVIGLTAKGYASTTTLDDKCGTVRVKSPQFINAEWLYNI 65

Query: 66  KLTSPQLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHR 125
             T   +    +Y    EN  ++     ++ NPFV+ A+ Y +   +  F   ++     
Sbjct: 66  DWTVSPIRVHQIY----ENCELE-----KNPNPFVQLALFYVI---NYFFSTGRQP---- 125

Query: 126 LLLQGLDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCK-PE 185
           L  Q L +T+   N +Y           P       S+P F          N  NC   +
Sbjct: 126 LCWQDLQVTLQVDNAYYHQ---------PQLKPDQTSYPKF----------NFLNCTLGQ 185

Query: 186 VAKTGLGSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLD--MVHVIAQTAHCIA 245
           V KTGLGSSAAM TS++ +L  +  +  L+   G DK  KI D    +VH +AQ AHC A
Sbjct: 186 VHKTGLGSSAAMITSLIGSL--FLSLRRLTDDTG-DKSLKIDDSTKVIVHNLAQIAHCSA 245

Query: 246 QGKVGSGFDVSSAVYGSQRYVRFSP----DVLAPAQAAVKG-PLEEVIVNILKGNWDHER 305
           QGKVGSGFDV +A +GS  Y RF P     +L P    +K       +  I+   W  + 
Sbjct: 246 QGKVGSGFDVGAATWGSCIYRRFDPKLIEQLLVPYDEQIKNINFSTELRKIVSKKWS-DV 305

Query: 306 TEFSLPPLMNLLLGEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLN 365
             F LP    LL+G+  AGGSSTP MV  V++WQK +P++S+  ++ L    L ++N   
Sbjct: 306 VPFQLPATYCLLMGDV-AGGSSTPGMVKKVQQWQKENPEESKNCFDDLYSRVLSIKNCF- 365

Query: 366 LLRKLAAEHWDVYKCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQ 425
               L++E  D         S L+S+                           IR  +++
Sbjct: 366 ----LSSESLD---------SELQSQ------------------------FRSIRRILQR 425

Query: 426 MGEAAGVPIEPKSQSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDS-CSSHVINSWT 485
           +   A V IEP  Q+ +LD   +L GV+  GVPGAGGFDA F + +  +    +VI +W 
Sbjct: 426 ITVEAKVDIEPLKQTNILDNIEQLPGVIGVGVPGAGGFDAQFCLAINHTEIIENVIKTWK 426

Query: 486 SLNVLPLLLKEDPNGVALQ 489
              V+P+ +    +G+A++
Sbjct: 486 DDGVVPMDVSPAFDGLAVE 426

BLAST of MS021081 vs. ExPASy Swiss-Prot
Match: P24521 (Phosphomevalonate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ERG8 PE=1 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 2.8e-40
Identity = 143/462 (30.95%), Postives = 216/462 (46.75%), Query Frame = 0

Query: 3   RVASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTS 62
           R  SAPGK LL GGYL+L+      V+  +AR +A+      H      G +  +V++ S
Sbjct: 5   RAFSAPGKALLAGGYLVLDTKYEAFVVGLSARMHAVA-----HPYGSLQGSDKFEVRVKS 64

Query: 63  PQL-SRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLL 122
            Q    E LY +S ++  +  VS   S+NPF+E  +    A     F  N  D  +R L 
Sbjct: 65  KQFKDGEWLYHISPKSGFIP-VSIGGSKNPFIEKVI----ANVFSYFKPNMDDYCNRNLF 124

Query: 123 QGLDITILGCNDFYSYRNQI-EARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAK 182
               I I   + ++S  + + E RG                   N   S   +   EV K
Sbjct: 125 V---IDIFSDDAYHSQEDSVTEHRG-------------------NRRLSFHSHRIEEVPK 184

Query: 183 TGLGSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKI-KDLDMVHVIAQTAHCIAQGKV 242
           TGLGSSA + T +  AL ++F           D EN + K  +++H +AQ AHC AQGK+
Sbjct: 185 TGLGSSAGLVTVLTTALASFF---------VSDLENNVDKYREVIHNLAQVAHCQAQGKI 244

Query: 243 GSGFDVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNIL-KGNWDHERTEFSLPPL 302
           GSGFDV++A YGS RY RF P +++             + +++ + +W+       LP  
Sbjct: 245 GSGFDVAAAAYGSIRYRRFPPALISNLPDIGSATYGSKLAHLVDEEDWNITIKSNHLPSG 304

Query: 303 MNLLLGEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAE 362
           + L +G+    GS T  +V  VK W  S   +S + + +L  AN    + L+ L +L   
Sbjct: 305 LTLWMGDI-KNGSETVKLVQKVKNWYDSHMPESLKIYTELDHANSRFMDGLSKLDRLHET 364

Query: 363 HWDVYKCIISKCSTLRSKKWMEQATEPSQQTIVK--ALLGARDAMLEIRSNMRQMGEAAG 422
           H D    I              ++ E +  T  K   +   RDA+  IR + R++ + +G
Sbjct: 365 HDDYSDQIF-------------ESLERNDCTCQKYPEITEVRDAVATIRRSFRKITKESG 411

Query: 423 VPIEPKSQSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGD 459
             IEP  Q+ +LD    L+GVL   +PGAGG+DA+  IT  D
Sbjct: 425 ADIEPPVQTSLLDDCQTLKGVLTCLIPGAGGYDAIAVITKQD 411

BLAST of MS021081 vs. ExPASy Swiss-Prot
Match: Q97UL6 (Phosphomevalonate kinase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=SSO2988 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.1e-07
Identity = 83/313 (26.52%), Postives = 132/313 (42.17%), Query Frame = 0

Query: 180 KTGLGSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKV 239
           KTGLGSS+A T S+ A L  Y+ +             K+ DL  +H +AQ A+   Q  +
Sbjct: 97  KTGLGSSSAATVSLTACL--YYAI-----------HGKL-DLFEIHKLAQIANYKRQKGI 156

Query: 240 GSGFDVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLM 299
           GSGFD++SAV+GS  Y RF+              ++     +  GN+D            
Sbjct: 157 GSGFDIASAVFGSIVYKRFTD----------LDKMDFYFEKLNLGNYD------------ 216

Query: 300 NLLLGEPGAGGSSTPSMVGAVKKW-QKSDPQKSQETWNKLSEANLDLENQLNLLRKL--A 359
            ++LG  G    +    VG V+K+ +KS+    +E    + E N  +  +L  L KL  A
Sbjct: 217 -MMLGFTGKSSET----VGLVRKFVEKSNLDDFKEIMRLIDEENY-MAIKLIKLNKLDEA 276

Query: 360 AEHWDVYKCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAG 419
            EH  +             +K++    E                               G
Sbjct: 277 VEHIKL------------GRKYLNYIAE----------------------------RIVG 322

Query: 420 VPIEPKSQSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPL 479
           V +  K + E++    E EG L+   PGAGG D++FA  LG+   + V  +W+   +  +
Sbjct: 337 VKLVSKMEEELIKIAEE-EGALVALSPGAGGGDSIFA--LGNDL-NRVREAWSKRGIFII 322

Query: 480 LLKEDPNGVALQT 490
            +KED  G+ L++
Sbjct: 397 DVKED-EGLRLES 322

BLAST of MS021081 vs. ExPASy TrEMBL
Match: A0A6J1CWL8 (Phosphomevalonate kinase OS=Momordica charantia OX=3673 GN=LOC111014891 PE=4 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 2.3e-282
Identity = 494/496 (99.60%), Postives = 496/496 (100.00%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP
Sbjct: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG
Sbjct: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASI+FNAEESNSENCKPEVAKTGL
Sbjct: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASISFNAEESNSENCKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF
Sbjct: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303
           DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL
Sbjct: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303

Query: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363
           GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY
Sbjct: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363

Query: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423
           KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS
Sbjct: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423

Query: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPN 483
           QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDP+
Sbjct: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPD 483

Query: 484 GVALQTADPRTNNIAS 500
           GVALQTADPRTNNIAS
Sbjct: 484 GVALQTADPRTNNIAS 499

BLAST of MS021081 vs. ExPASy TrEMBL
Match: K7NBK6 (Phosphomevalonate kinase OS=Siraitia grosvenorii OX=190515 GN=PMVK PE=2 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 5.2e-255
Identity = 448/495 (90.51%), Postives = 467/495 (94.34%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEH EPESGGG+WVDVKLTSP
Sbjct: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHTEPESGGGKWVDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QL RESLY+LS ENFT+QC+SS E RNPFV+HAVQYS+AA HGIFD NKKDSLHRLLLQG
Sbjct: 64  QLCRESLYKLSPENFTIQCISSIELRNPFVKHAVQYSVAAAHGIFDKNKKDSLHRLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL
Sbjct: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTT+VVAALLNY GVVNLSS +GQDKEN IKDLDMVHVIAQTAHCIAQGKVGSGF
Sbjct: 184 GSSAAMTTAVVAALLNYLGVVNLSSPRGQDKENDIKDLDMVHVIAQTAHCIAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303
           DV SAVYGSQRYVRFSP+VLA AQ AVKGPLEEVIVNILKG WDHERTEFSLPPLMNLLL
Sbjct: 244 DVCSAVYGSQRYVRFSPEVLATAQVAVKGPLEEVIVNILKGKWDHERTEFSLPPLMNLLL 303

Query: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363
           GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANL LE QLNLLRKLAAEHWDVY
Sbjct: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLGLEKQLNLLRKLAAEHWDVY 363

Query: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423
           KCIIS CS L SKKWMEQATE SQQ IVKALLGARDAMLEIR++MRQMGEAAGVPIEPKS
Sbjct: 364 KCIISGCSILGSKKWMEQATEQSQQAIVKALLGARDAMLEIRNHMRQMGEAAGVPIEPKS 423

Query: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPN 483
           Q+++LDATMELEGVLL GVPGAGGFDAVFAITLGDS S HVI SWTSLNVL LL++EDPN
Sbjct: 424 QTQLLDATMELEGVLLAGVPGAGGFDAVFAITLGDS-SRHVIESWTSLNVLALLVREDPN 483

Query: 484 GVALQTADPRTNNIA 499
           GVAL++ DPR+ NIA
Sbjct: 484 GVALESGDPRSKNIA 497

BLAST of MS021081 vs. ExPASy TrEMBL
Match: A0A6J1GJ21 (Phosphomevalonate kinase OS=Cucurbita moschata OX=3662 GN=LOC111454316 PE=4 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 4.0e-247
Identity = 435/495 (87.88%), Postives = 462/495 (93.33%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           V SAPGKVLLTGGYLILERPN GLVLSTNARFYAIVKPLHE  E ESG GEWVDVKLTSP
Sbjct: 4   VTSAPGKVLLTGGYLILERPNPGLVLSTNARFYAIVKPLHEQTEAESGSGEWVDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QLSRESLY+LSL++FT+QC+S SES+N FVEHA+QYSLA  HGI D NKKDSLHRLLLQG
Sbjct: 64  QLSRESLYKLSLKSFTIQCISLSESKNLFVEHALQYSLAVAHGILDKNKKDSLHRLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILGCNDFYSYRNQIEARGLPLTPESL+SF PFASITFNAEES+SENCKPEVAKTGL
Sbjct: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLLSFTPFASITFNAEESSSENCKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTT+VVAALLNY GVVNLSSS+GQDKEN IKDLD+VHVIAQTAHCIAQGKVGSGF
Sbjct: 184 GSSAAMTTAVVAALLNYLGVVNLSSSRGQDKENDIKDLDIVHVIAQTAHCIAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303
           DVSSAVYGSQRYVRFSP+VL  AQAAVK PLEEVIV+ILKG WDHERTEFSLPPLMNLLL
Sbjct: 244 DVSSAVYGSQRYVRFSPNVLTTAQAAVKEPLEEVIVDILKGKWDHERTEFSLPPLMNLLL 303

Query: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363
           GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLE QLNLLRK+AAE WDVY
Sbjct: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLERQLNLLRKVAAEQWDVY 363

Query: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423
           KCI+SKCS L SKKWMEQATEP+QQ IVKALLGARDAMLEIR++MRQMGEAAG+PIEPKS
Sbjct: 364 KCILSKCSMLGSKKWMEQATEPNQQGIVKALLGARDAMLEIRNHMRQMGEAAGIPIEPKS 423

Query: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPN 483
           Q+E+LD TMELEGVLL GVPGAGGFDAVFAITLGDS SSHVI SWTSLNVL LL++EDPN
Sbjct: 424 QTELLDRTMELEGVLLAGVPGAGGFDAVFAITLGDS-SSHVIKSWTSLNVLALLVREDPN 483

Query: 484 GVALQTADPRTNNIA 499
           GVAL+  DPR+  +A
Sbjct: 484 GVALERGDPRSKCMA 497

BLAST of MS021081 vs. ExPASy TrEMBL
Match: A0A6J1KYD3 (Phosphomevalonate kinase OS=Cucurbita maxima OX=3661 GN=LOC111498684 PE=4 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.4e-246
Identity = 432/495 (87.27%), Postives = 459/495 (92.73%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           V SAPGKVLLTGGYLILERPN GLVLSTNARFYAIVKPLHE  E ESG GEWVDVKLTSP
Sbjct: 4   VTSAPGKVLLTGGYLILERPNPGLVLSTNARFYAIVKPLHEQTEAESGSGEWVDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QLSRESLY+LSLENFT+Q +  SES+N FVEHA+QYSLAA HGIFD NKKDSLHRLLLQG
Sbjct: 64  QLSRESLYKLSLENFTIQSIGLSESKNLFVEHALQYSLAAAHGIFDKNKKDSLHRLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDIT+LGCNDFYSYRNQIEARGLPLTPESL+SF PFASITFNAEES+SENCKPEVAKTGL
Sbjct: 124 LDITVLGCNDFYSYRNQIEARGLPLTPESLLSFTPFASITFNAEESSSENCKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTT+VVAALLNY GVVNLSSS+GQDKEN  KDLD+VHVIAQTAHCIAQGKVGSGF
Sbjct: 184 GSSAAMTTAVVAALLNYLGVVNLSSSRGQDKENDSKDLDIVHVIAQTAHCIAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEFSLPPLMNLLL 303
           D+SSAVYGSQRYVRFSPDVL  AQ AVK PLEEVIV ILKG WDHERTEFSLPPLMNLLL
Sbjct: 244 DISSAVYGSQRYVRFSPDVLTTAQTAVKEPLEEVIVGILKGKWDHERTEFSLPPLMNLLL 303

Query: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDVY 363
           GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDL+ QLNLLRK+AAE WDVY
Sbjct: 304 GEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLKRQLNLLRKVAAEQWDVY 363

Query: 364 KCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPKS 423
           KCI+SKCS L SKKWMEQATEP+QQ IVKALLGARDAMLEIR++MRQMGEAAG+PIEPKS
Sbjct: 364 KCILSKCSMLGSKKWMEQATEPNQQAIVKALLGARDAMLEIRNHMRQMGEAAGIPIEPKS 423

Query: 424 QSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDPN 483
           Q+E+LD TMELEGVL+ GVPGAGGFDAVFAITLGDS SSHVI SWTSLNVL LL++EDPN
Sbjct: 424 QTELLDRTMELEGVLVAGVPGAGGFDAVFAITLGDS-SSHVIQSWTSLNVLALLVREDPN 483

Query: 484 GVALQTADPRTNNIA 499
           GVAL+  DPR+  +A
Sbjct: 484 GVALECGDPRSKCMA 497

BLAST of MS021081 vs. ExPASy TrEMBL
Match: A0A6J1GJ29 (Phosphomevalonate kinase OS=Cucurbita moschata OX=3662 GN=LOC111454316 PE=4 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 2.9e-221
Identity = 391/445 (87.87%), Postives = 418/445 (93.93%), Query Frame = 0

Query: 54  EWVDVKLTSPQLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKK 113
           EWVDVKLTSPQLSRESLY+LSL++FT+QC+S SES+N FVEHA+QYSLA  HGI D NKK
Sbjct: 17  EWVDVKLTSPQLSRESLYKLSLKSFTIQCISLSESKNLFVEHALQYSLAVAHGILDKNKK 76

Query: 114 DSLHRLLLQGLDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSEN 173
           DSLHRLLLQGLDITILGCNDFYSYRNQIEARGLPLTPESL+SF PFASITFNAEES+SEN
Sbjct: 77  DSLHRLLLQGLDITILGCNDFYSYRNQIEARGLPLTPESLLSFTPFASITFNAEESSSEN 136

Query: 174 CKPEVAKTGLGSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHC 233
           CKPEVAKTGLGSSAAMTT+VVAALLNY GVVNLSSS+GQDKEN IKDLD+VHVIAQTAHC
Sbjct: 137 CKPEVAKTGLGSSAAMTTAVVAALLNYLGVVNLSSSRGQDKENDIKDLDIVHVIAQTAHC 196

Query: 234 IAQGKVGSGFDVSSAVYGSQRYVRFSPDVLAPAQAAVKGPLEEVIVNILKGNWDHERTEF 293
           IAQGKVGSGFDVSSAVYGSQRYVRFSP+VL  AQAAVK PLEEVIV+ILKG WDHERTEF
Sbjct: 197 IAQGKVGSGFDVSSAVYGSQRYVRFSPNVLTTAQAAVKEPLEEVIVDILKGKWDHERTEF 256

Query: 294 SLPPLMNLLLGEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLR 353
           SLPPLMNLLLGEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLE QLNLLR
Sbjct: 257 SLPPLMNLLLGEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLERQLNLLR 316

Query: 354 KLAAEHWDVYKCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGE 413
           K+AAE WDVYKCI+SKCS L SKKWMEQATEP+QQ IVKALLGARDAMLEIR++MRQMGE
Sbjct: 317 KVAAEQWDVYKCILSKCSMLGSKKWMEQATEPNQQGIVKALLGARDAMLEIRNHMRQMGE 376

Query: 414 AAGVPIEPKSQSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNV 473
           AAG+PIEPKSQ+E+LD TMELEGVLL GVPGAGGFDAVFAITLGDS SSHVI SWTSLNV
Sbjct: 377 AAGIPIEPKSQTELLDRTMELEGVLLAGVPGAGGFDAVFAITLGDS-SSHVIKSWTSLNV 436

Query: 474 LPLLLKEDPNGVALQTADPRTNNIA 499
           L LL++EDPNGVAL+  DPR+  +A
Sbjct: 437 LALLVREDPNGVALERGDPRSKCMA 460

BLAST of MS021081 vs. TAIR 10
Match: AT1G31910.1 (GHMP kinase family protein )

HSP 1 Score: 682.2 bits (1759), Expect = 3.2e-196
Identity = 348/497 (70.02%), Postives = 411/497 (82.70%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           VASAPGKVL+TGGYL+LE+PNAGLVLSTNARFYAIVKP++E  +PES   +W DVKLTSP
Sbjct: 4   VASAPGKVLMTGGYLVLEKPNAGLVLSTNARFYAIVKPINEEVKPESWAWKWTDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QLSRES+Y+LSL + T+Q VS+S+SRNPFVEHA+QY++AA H +     K+SLH+LLLQG
Sbjct: 64  QLSRESMYKLSLNHLTLQSVSASDSRNPFVEHAIQYAIAAAH-LATEKDKESLHKLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILG NDFYSYRNQIE+ GLPLTPESL +  PFASITFNA ESN  N KPEVAKTGL
Sbjct: 124 LDITILGSNDFYSYRNQIESAGLPLTPESLGTLAPFASITFNAAESNGANSKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTT+VVAALL+Y GVV+LS    + K     DLD++H+IAQT+HC+AQGKVGSGF
Sbjct: 184 GSSAAMTTAVVAALLHYLGVVDLSDPCKEGKFG-CSDLDVIHMIAQTSHCLAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKG-PLEEVIVNILKGNWDHERTEFSLPPLMNLL 303
           DVS AVYGSQRYVRFSP+VL+ AQ AV G PL EVI  ILKG WD++RTEFSLPPLMNL 
Sbjct: 244 DVSCAVYGSQRYVRFSPEVLSFAQVAVTGLPLNEVIGTILKGKWDNKRTEFSLPPLMNLF 303

Query: 304 LGEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDV 363
           LGEPG+GGSSTPSMVGAVKKWQ SDP+K++E W  LS+ANL+LE +LN L KLA +HWDV
Sbjct: 304 LGEPGSGGSSTPSMVGAVKKWQMSDPEKARENWQNLSDANLELETKLNDLSKLAKDHWDV 363

Query: 364 YKCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVPIEPK 423
           Y  +I  CS L S+KW+  ATEP  + I+K LL AR+AML IR  MRQMGEAA VPIEP+
Sbjct: 364 YLRVIKSCSVLTSEKWVLHATEPINEAIIKELLEAREAMLRIRILMRQMGEAASVPIEPE 423

Query: 424 SQSEVLDATMELEGVLLGGVPGAGGFDAVFAITLGDSCSSHVINSWTSLNVLPLLLKEDP 483
           SQ+++LD+TM  EGVLL GVPGAGGFDA+FAITLGDS  + +  +W+S NVL LL++EDP
Sbjct: 424 SQTQLLDSTMSAEGVLLAGVPGAGGFDAIFAITLGDS-GTKLTQAWSSHNVLALLVREDP 483

Query: 484 NGVALQTADPRTNNIAS 500
           +GV L++ DPRT  I S
Sbjct: 484 HGVCLESGDPRTTCITS 497

BLAST of MS021081 vs. TAIR 10
Match: AT1G31910.2 (GHMP kinase family protein )

HSP 1 Score: 582.4 bits (1500), Expect = 3.4e-166
Identity = 297/416 (71.39%), Postives = 345/416 (82.93%), Query Frame = 0

Query: 4   VASAPGKVLLTGGYLILERPNAGLVLSTNARFYAIVKPLHEHAEPESGGGEWVDVKLTSP 63
           VASAPGKVL+TGGYL+LE+PNAGLVLSTNARFYAIVKP++E  +PES   +W DVKLTSP
Sbjct: 4   VASAPGKVLMTGGYLVLEKPNAGLVLSTNARFYAIVKPINEEVKPESWAWKWTDVKLTSP 63

Query: 64  QLSRESLYRLSLENFTVQCVSSSESRNPFVEHAVQYSLAATHGIFDNNKKDSLHRLLLQG 123
           QLSRES+Y+LSL + T+Q VS+S+SRNPFVEHA+QY++AA H +     K+SLH+LLLQG
Sbjct: 64  QLSRESMYKLSLNHLTLQSVSASDSRNPFVEHAIQYAIAAAH-LATEKDKESLHKLLLQG 123

Query: 124 LDITILGCNDFYSYRNQIEARGLPLTPESLISFPPFASITFNAEESNSENCKPEVAKTGL 183
           LDITILG NDFYSYRNQIE+ GLPLTPESL +  PFASITFNA ESN  N KPEVAKTGL
Sbjct: 124 LDITILGSNDFYSYRNQIESAGLPLTPESLGTLAPFASITFNAAESNGANSKPEVAKTGL 183

Query: 184 GSSAAMTTSVVAALLNYFGVVNLSSSKGQDKENKIKDLDMVHVIAQTAHCIAQGKVGSGF 243
           GSSAAMTT+VVAALL+Y GVV+LS    + K     DLD++H+IAQT+HC+AQGKVGSGF
Sbjct: 184 GSSAAMTTAVVAALLHYLGVVDLSDPCKEGKFG-CSDLDVIHMIAQTSHCLAQGKVGSGF 243

Query: 244 DVSSAVYGSQRYVRFSPDVLAPAQAAVKG-PLEEVIVNILKGNWDHERTEFSLPPLMNLL 303
           DVS AVYGSQRYVRFSP+VL+ AQ AV G PL EVI  ILKG WD++RTEFSLPPLMNL 
Sbjct: 244 DVSCAVYGSQRYVRFSPEVLSFAQVAVTGLPLNEVIGTILKGKWDNKRTEFSLPPLMNLF 303

Query: 304 LGEPGAGGSSTPSMVGAVKKWQKSDPQKSQETWNKLSEANLDLENQLNLLRKLAAEHWDV 363
           LGEPG+GGSSTPSMVGAVKKWQ SDP+K++E W  LS+ANL+LE +LN L KLA +HWDV
Sbjct: 304 LGEPGSGGSSTPSMVGAVKKWQMSDPEKARENWQNLSDANLELETKLNDLSKLAKDHWDV 363

Query: 364 YKCIISKCSTLRSKKWMEQATEPSQQTIVKALLGARDAMLEIRSNMRQMGEAAGVP 419
           Y  +I  CS L S+KW+  ATEP  + I+K LL AR+AML IR  MRQMGEAA VP
Sbjct: 364 YLRVIKSCSVLTSEKWVLHATEPINEAIIKELLEAREAMLRIRILMRQMGEAASVP 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145447.14.7e-28299.60phosphomevalonate kinase, peroxisomal-like [Momordica charantia][more]
AEM42973.11.1e-25490.51phosphomevalonate kinase [Siraitia grosvenorii][more]
XP_022951530.18.3e-24787.88phosphomevalonate kinase, peroxisomal-like isoform X1 [Cucurbita moschata][more]
KAG6596708.11.1e-24687.68Phosphomevalonate kinase, peroxisomal, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023540458.13.2e-24687.27phosphomevalonate kinase, peroxisomal-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9C6T14.5e-19570.02Phosphomevalonate kinase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=PMK PE=... [more]
Q9UT881.4e-4231.46Probable phosphomevalonate kinase OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
P245212.8e-4030.95Phosphomevalonate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c... [more]
Q97UL61.1e-0726.52Phosphomevalonate kinase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM ... [more]
Match NameE-valueIdentityDescription
A0A6J1CWL82.3e-28299.60Phosphomevalonate kinase OS=Momordica charantia OX=3673 GN=LOC111014891 PE=4 SV=... [more]
K7NBK65.2e-25590.51Phosphomevalonate kinase OS=Siraitia grosvenorii OX=190515 GN=PMVK PE=2 SV=1[more]
A0A6J1GJ214.0e-24787.88Phosphomevalonate kinase OS=Cucurbita moschata OX=3662 GN=LOC111454316 PE=4 SV=1[more]
A0A6J1KYD33.4e-24687.27Phosphomevalonate kinase OS=Cucurbita maxima OX=3661 GN=LOC111498684 PE=4 SV=1[more]
A0A6J1GJ292.9e-22187.87Phosphomevalonate kinase OS=Cucurbita moschata OX=3662 GN=LOC111454316 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G31910.13.2e-19670.02GHMP kinase family protein [more]
AT1G31910.23.4e-16671.39GHMP kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 338..358
NoneNo IPR availablePANTHERPTHR31814:SF6SUBFAMILY NOT NAMEDcoord: 4..498
IPR036554GHMP kinase, C-terminal domain superfamilyGENE3D3.30.70.890coord: 307..477
e-value: 1.0E-5
score: 27.7
IPR016005Phosphomevalonate kinase Erg8PIRSFPIRSF017288PMK_GHMP_eukcoord: 1..497
e-value: 6.1E-194
score: 642.9
IPR016005Phosphomevalonate kinase Erg8TIGRFAMTIGR01219TIGR01219coord: 4..494
e-value: 5.5E-170
score: 564.1
IPR013750GHMP kinase, C-terminal domainPFAMPF08544GHMP_kinases_Ccoord: 401..470
e-value: 4.8E-7
score: 30.1
IPR014721Ribosomal protein S5 domain 2-type fold, subgroupGENE3D3.30.230.10coord: 3..306
e-value: 1.1E-36
score: 128.4
IPR006204GHMP kinase N-terminal domainPFAMPF00288GHMP_kinases_Ncoord: 174..252
e-value: 4.3E-17
score: 62.1
IPR035102Phosphomevalonate kinasePANTHERPTHR31814FAMILY NOT NAMEDcoord: 4..498
IPR020568Ribosomal protein S5 domain 2-type foldSUPERFAMILY54211Ribosomal protein S5 domain 2-likecoord: 4..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS021081.1MS021081.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005524 ATP binding
molecular_function GO:0004631 phosphomevalonate kinase activity