MS021063 (gene) Bitter gourd (TR) v1

Overview
NameMS021063
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold290: 944800 .. 949020 (+)
RNA-Seq ExpressionMS021063
SyntenyMS021063
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCAGCACAGGAGGCGTAATGAAATTATTTTGTGAGATAGGTGTTGGATCCTTAGTGCAACTGTTCAAGTAAGGACTATATTTGATAACTTTTTTGTGAGAATTTTGATATCTAGTTTCATTTAATCATAATATTCTCTCTTTTTATTTTATTTTGTATCCAGAGCGTTGTTGATGCATCATCCAGACGATTTTCTAGGGGAGACTTCTGCAGGGGTATGATGAAAAATTTACTTTCCTTCTTTTGATTTTTCTTGTTAATTTGTTTTATGCAATGCTAACCTCAGTTTTTTCCCCTGCAGAGCTGAGTTCTGTTGATGAAACATTGAAGCTTGTTTTCACAATTACCAACAGTCAAGACCATTGCTTTGACCTCTGTTGGTCAAGCTTTTATTATTTGTTCGTTATCTTTGTTACTGATGGGTGATGTTTAGAGAGTCATGACATTGCTTACTGGAGGATCTATCTTCCATTTCTATGTTGAATTATGAGGTAGCTGCAACTTAAGGTTGTCAACGTCAGGGAGAGTGTCTCGTACTTCAGGTATTTCCAACTGCTCAGTTGGTTTGGACAGTTCTTCTCAAGATTCAACATGTTCTGTGGTATTTGTAATAATTCCTGATCTTGTTCCTTTCTTGAAGTTGAAGCTGCGGATATTGTAATGAATAAAGCTGCACAGCCTGCACATCAGAATTACTCTGGTTTTGTACTTCTGCATTTCACCATGAAAAATTAGTGGTGGTTTTTGCTAGATCAGTGCAAGACATTTGTTTACATTTAGTCTTTGTACAAAGATAAATGCAAGTTATCAATTCAAGATTCTCTCTCTCTTTTGAATAAAGGAGGTTGTCTTTGTGCTTCTGCAAGAAAAATGGAGAGGTCGGTTGGTTTCCATAGCTGAACTATTTTCCACTCATTTTGTTGTACAATGTGCTTCAATGGCTTTATTACTTTATTTATGCTTGTCAATTTTGTAATTATTTTAAAGGTACATGTTAATCAAGGAAGTTGGTGATGGTACTTTTGGAAGTGTCTGGCGAGCTATCAATAAGCAGACTGGTGAAGTTGTAAGTTTCCTGATTTTCTCTCTCCATGCTTGTATCATGAGAAAAGATTGTCTGTTAAGCAAGGTAGTTTTCATTTTAATAATCTGATCCTTTTAATCACCAGGTTGCGATTAAGAAAATGAAGAAAAAATATTACACTTGGGAGGAGTGTGTCAATCTGAGAGAAGTAAAGGTGATGCATTCTTGATTTTTTTTAATTATTCTGACTTCCTCATTCTTCTTTCCTTAGACATTAATAATATTTTGTTATTCTAATATGACAGTCATTACGCAAGATGAATCATCCAAATATCGTGAAACTCAAGGAAGTTATCAGGGAAAACAATATCTTGTACTTTGTGTTTGAATACATGGTTAGTAACTTACTTTTGCAACTCATTATGTTTTTTTTTTGTCAAAGGTTATCTTGACTTGCAACTTTTATGTTCAGGAATGCAACCTTTATCAACTTATGAAGGATAAGGAAAAGCTCTTCTCAGAAACTGAAGTCAGAAATTGGTGCTTTCAAGTTTTTCAAGGTCTTGCCTATATGCATCAGCGAGGATATTTTCATCGTGATCTTAAACCAGGTATTTCTATAATGATTTAAGAGCTAACTGATTAGCAGTCTAGAAATATCTCTCAACTCTCTATGAGGGTGTACATTGTTTCAGTGCCACCAATGTGAAGTGTATATCTTTGTGTTTCTATGTTTGGGGAGCTCAACAATTTATTATGTCTTGCCGTTCCTCCATTGTTTTTGTGTCCAGTAGAATGCACTGAAGTTGGAGCTGGACATCCATTTTACCTACTGTAAACACCTTGAATTTGATTTGCATACTTTCCTAGGTTATTTTGAGTTCCTATGCTCTGTAGAAGTTTTACATTATTTGGGTGCAATTTCACTTGATAAAATATAGAATAGTAGCTTTCTTTATGGTTCTGGAAGGATTTTTTAGATCAAGTTCTAGGGGATCGTATGATGGTATAGGTAAATGTTAAATGAATACTTTGTTGTGTGTGCATCATTGCGATGATTTGTTTTTGTTTTGTGATGCCATGGTGTTTGTTTTCTGTTTGTTTCATAGCTCACTATAACTCGTGCCACATCACATGTTTCTTGTTAGTTTTTTTAAACCTTTTTTTGGTTATTCAGTATACTTACTATAGTTTCTTTTGAGTTGTAATTAACATGAGCCTTTTTTGCAGAAAATTTATTAGTTGCTAAAGAATTAATAAAACTGGCTGATTTTGGGCTTGCACGTGAAATTAGCGCAATGCCACCCTATACTGAGTATGTTTCAACACGGTGGTAAGTCCATTTCATCCCTTGCCTGTTTTTTATTTTGTTGCAAAGTATTTCCTTTATCCAATCATGGTAATATTATATTGTAACTTTTAATTATTGGGTTTCAGGTATAGAGCTCCAGAGGTGCTGCTTCAGTCCTATCTTTATGGTCCTAAAGTTGGTGAGGAACTTTTTAGCTGCTCCAAATAAAACTTAAACCAATTGATGCTTACTACTTACATTCTTTTTCCAGATATGTGGGCAATGGGTGCTATCATGGCAGAGTTGTTTACCCTTCGTCCTCTTTTTCCTGGGGCAAGGTATGCCCACCAACTTTGTCTCTCATGCCTTTCATTCTACATGCCAGGAGAAATTGCCAGCTTATTACAAGAGTTTGTTTTATTTGTCTCATGCAGTGAAATAGACCAAATCTACAAAATTTGCAGTATACTTGGCACCCCAACCATGGATTCATGGTCTGCTGGGCTCTCTCTTGCAAGGAATATCAGTTATGAATTCCCGCAGGTTTGTCTTCTTTGTCTTTTTTATCCTGCCTGTAACTTTTGTTAGAACAACCATGTAAATGCTTGGTATTTAATGTATTTGCTTGTTTCACAAATGCAGTTTGGTGGAGTTCATCTCTCTGTTGTTATTCCTGCAGCCAGTGATGATGCAGTCAATCTTATTGCAGTAAGTACAATAGTGGATGGCCGTTGAACTATCTTGTCTTGAACTGTTTAATTCTTAAAACAACACTACTGAGCTTGTGTTTTTAATTTGAATACTGCACCTCCTTATTTGTCGTGTCTGCAGTCACTCTGTTCATGGGATCCTTCGAAGAGACCAACAGCTATGGAAGCCCTCCAACATCCCTTCTTCCAAGTGAGTAGTCCCTTGGCTTTCATCCATTTGATTCGTAACTTTGTATTATTTTATTTATGGTTTTCTTATATTTGTTTAATGTGGCAGAGTTGCTACTACGTACCACCATCTCTACGTGCTAGAGTCCCCATTTCTCGAACTCCTCCTTCTGGTATGTCCCCTGTATCTTAGAAAACATTGCGACCTGGACTTTCTCCGATATGGAAATTTGTAAAGTGTTTTTCTTCACTGTTGCAGCTGGAACAAAAGATTTTCTGGAGCAACAAACTGCTAAAAAATTTCCTGGGACTTTATCTGATTCAAGGGTTGCTGGTAATTTCTCCTCTCCAAAACAGCCTGGTGCACTGTTGGGCACAGGTAGTATATTTATTCCTCGGTGGAATCTGTATGTTTTACTGCATCAGATCTTGAACCGAATACTTATTTCTCTAAACCATATTGCAGGTGTGCAGAGGAAGTTGGATTTGCTCAACCAGGTGAGAACACTGTCATCCTTTCCTGCCCCTTCACGCAGAGAAATTAAAGAAAATAATGTCGAAATGATTCTAATCTTTTCTTTTTGTTATCGTTTTTCTTGAAGGATGCGAATAAGAATGATAAAATTATGAAGAACACAGTTAGACAACAGAAATACCGACCGCCTGTGAGGAATAGCCCTAATAGTAAGTTTCATGGTGCGTGTTTCTGTTACACTACTAGCAATGATTTTATACTTATACTCGATTTCAGTTATCAGCTTAGATTAGTCTATGAAGCGGATTGTAATTTTGTTGAACTCGTGCAAATTGCAGACTCTATCTACAAGGGAAGTACCATGCGTGGAGTTTCAGATACAGGCGAAAAGTTGGCAAACATGACAATCGCCGCTCGCAAGCCATCTATCGGACAGCTTCCCCGGCCGATGAAAGCGGGCGTCCGTTGGAGCGGATCCTCTAGCGATCTGTTACTGAGGCCCGCCCAAGAGATTCAACATGGCAGCAATTACTCCAGGAAGGTTGCAGGA

mRNA sequence

ATGATCAGCACAGGAGGCGTAATGAAATTATTTTGTGAGATAGGTGTTGGATCCTTAGTGCAACTGTTCAAAGCGTTGTTGATGCATCATCCAGACGATTTTCTAGGGGAGACTTCTGCAGGGATAAATGCAAGTTATCAATTCAAGTCTCTCTCTCTTTTGAATAAAGGAGGTTGTCTTTGTGCTTCTGCAAGAAAAATGGAGAGGTACATGTTAATCAAGGAAGTTGGTGATGGTACTTTTGGAAGTGTCTGGCGAGCTATCAATAAGCAGACTGGTGAAGTTGTTGCGATTAAGAAAATGAAGAAAAAATATTACACTTGGGAGGAGTGTGTCAATCTGAGAGAAGTAAAGTCATTACGCAAGATGAATCATCCAAATATCGTGAAACTCAAGGAAGTTATCAGGGAAAACAATATCTTGTACTTTGTGTTTGAATACATGGAATGCAACCTTTATCAACTTATGAAGGATAAGGAAAAGCTCTTCTCAGAAACTGAAGTCAGAAATTGGTGCTTTCAAGTTTTTCAAGGTCTTGCCTATATGCATCAGCGAGGATATTTTCATCGTGATCTTAAACCAGAAAATTTATTAGTTGCTAAAGAATTAATAAAACTGGCTGATTTTGGGCTTGCACGTGAAATTAGCGCAATGCCACCCTATACTGAGTATGTTTCAACACGGTGGTATAGAGCTCCAGAGGTGCTGCTTCAGTCCTATCTTTATGGTCCTAAAGTTGATATGTGGGCAATGGGTGCTATCATGGCAGAGTTGTTTACCCTTCGTCCTCTTTTTCCTGGGGCAAGTGAAATAGACCAAATCTACAAAATTTGCAGTATACTTGGCACCCCAACCATGGATTCATGGTCTGCTGGGCTCTCTCTTGCAAGGAATATCAGTTATGAATTCCCGCAGTTTGGTGGAGTTCATCTCTCTGTTGTTATTCCTGCAGCCAGTGATGATGCAGTCAATCTTATTGCATCACTCTGTTCATGGGATCCTTCGAAGAGACCAACAGCTATGGAAGCCCTCCAACATCCCTTCTTCCAAAGTTGCTACTACGTACCACCATCTCTACGTGCTAGAGTCCCCATTTCTCGAACTCCTCCTTCTGCTGGAACAAAAGATTTTCTGGAGCAACAAACTGCTAAAAAATTTCCTGGGACTTTATCTGATTCAAGGGTTGCTGGTAATTTCTCCTCTCCAAAACAGCCTGGTGCACTGTTGGGCACAGGTAGTGTGCAGAGGAAGTTGGATTTGCTCAACCAGGATGCGAATAAGAATGATAAAATTATGAAGAACACAGTTAGACAACAGAAATACCGACCGCCTGTGAGGAATAGCCCTAATAACTCTATCTACAAGGGAAGTACCATGCGTGGAGTTTCAGATACAGGCGAAAAGTTGGCAAACATGACAATCGCCGCTCGCAAGCCATCTATCGGACAGCTTCCCCGGCCGATGAAAGCGGGCGTCCGTTGGAGCGGATCCTCTAGCGATCTGTTACTGAGGCCCGCCCAAGAGATTCAACATGGCAGCAATTACTCCAGGAAGGTTGCAGGA

Coding sequence (CDS)

ATGATCAGCACAGGAGGCGTAATGAAATTATTTTGTGAGATAGGTGTTGGATCCTTAGTGCAACTGTTCAAAGCGTTGTTGATGCATCATCCAGACGATTTTCTAGGGGAGACTTCTGCAGGGATAAATGCAAGTTATCAATTCAAGTCTCTCTCTCTTTTGAATAAAGGAGGTTGTCTTTGTGCTTCTGCAAGAAAAATGGAGAGGTACATGTTAATCAAGGAAGTTGGTGATGGTACTTTTGGAAGTGTCTGGCGAGCTATCAATAAGCAGACTGGTGAAGTTGTTGCGATTAAGAAAATGAAGAAAAAATATTACACTTGGGAGGAGTGTGTCAATCTGAGAGAAGTAAAGTCATTACGCAAGATGAATCATCCAAATATCGTGAAACTCAAGGAAGTTATCAGGGAAAACAATATCTTGTACTTTGTGTTTGAATACATGGAATGCAACCTTTATCAACTTATGAAGGATAAGGAAAAGCTCTTCTCAGAAACTGAAGTCAGAAATTGGTGCTTTCAAGTTTTTCAAGGTCTTGCCTATATGCATCAGCGAGGATATTTTCATCGTGATCTTAAACCAGAAAATTTATTAGTTGCTAAAGAATTAATAAAACTGGCTGATTTTGGGCTTGCACGTGAAATTAGCGCAATGCCACCCTATACTGAGTATGTTTCAACACGGTGGTATAGAGCTCCAGAGGTGCTGCTTCAGTCCTATCTTTATGGTCCTAAAGTTGATATGTGGGCAATGGGTGCTATCATGGCAGAGTTGTTTACCCTTCGTCCTCTTTTTCCTGGGGCAAGTGAAATAGACCAAATCTACAAAATTTGCAGTATACTTGGCACCCCAACCATGGATTCATGGTCTGCTGGGCTCTCTCTTGCAAGGAATATCAGTTATGAATTCCCGCAGTTTGGTGGAGTTCATCTCTCTGTTGTTATTCCTGCAGCCAGTGATGATGCAGTCAATCTTATTGCATCACTCTGTTCATGGGATCCTTCGAAGAGACCAACAGCTATGGAAGCCCTCCAACATCCCTTCTTCCAAAGTTGCTACTACGTACCACCATCTCTACGTGCTAGAGTCCCCATTTCTCGAACTCCTCCTTCTGCTGGAACAAAAGATTTTCTGGAGCAACAAACTGCTAAAAAATTTCCTGGGACTTTATCTGATTCAAGGGTTGCTGGTAATTTCTCCTCTCCAAAACAGCCTGGTGCACTGTTGGGCACAGGTAGTGTGCAGAGGAAGTTGGATTTGCTCAACCAGGATGCGAATAAGAATGATAAAATTATGAAGAACACAGTTAGACAACAGAAATACCGACCGCCTGTGAGGAATAGCCCTAATAACTCTATCTACAAGGGAAGTACCATGCGTGGAGTTTCAGATACAGGCGAAAAGTTGGCAAACATGACAATCGCCGCTCGCAAGCCATCTATCGGACAGCTTCCCCGGCCGATGAAAGCGGGCGTCCGTTGGAGCGGATCCTCTAGCGATCTGTTACTGAGGCCCGCCCAAGAGATTCAACATGGCAGCAATTACTCCAGGAAGGTTGCAGGA

Protein sequence

MISTGGVMKLFCEIGVGSLVQLFKALLMHHPDDFLGETSAGINASYQFKSLSLLNKGGCLCASARKMERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHPNIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRGYFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKVDMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQFGGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPISRTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDANKNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG
Homology
BLAST of MS021063 vs. NCBI nr
Match: XP_022145414.1 (cyclin-dependent kinase F-4 isoform X2 [Momordica charantia])

HSP 1 Score: 904.4 bits (2336), Expect = 4.4e-259
Identity = 451/455 (99.12%), Postives = 452/455 (99.34%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP
Sbjct: 1   MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFS TEVRNWCFQVFQGLAY+HQRG
Sbjct: 61  NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSVTEVRNWCFQVFQGLAYIHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV
Sbjct: 121 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF
Sbjct: 181 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
           GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS
Sbjct: 241 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RT PSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTG VQRKLDLLNQDAN
Sbjct: 301 RT-PSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTG-VQRKLDLLNQDAN 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR 486
           KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR
Sbjct: 361 KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR 420

Query: 487 PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
           PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG
Sbjct: 421 PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 453

BLAST of MS021063 vs. NCBI nr
Match: XP_022145412.1 (cyclin-dependent kinase F-4 isoform X1 [Momordica charantia] >XP_022145413.1 cyclin-dependent kinase F-4 isoform X1 [Momordica charantia])

HSP 1 Score: 896.7 bits (2316), Expect = 9.2e-257
Identity = 450/459 (98.04%), Postives = 452/459 (98.47%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP
Sbjct: 1   MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFS TEVRNWCFQVFQGLAY+HQRG
Sbjct: 61  NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSVTEVRNWCFQVFQGLAYIHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV
Sbjct: 121 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF
Sbjct: 181 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
           GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS
Sbjct: 241 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RT PSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTG VQRKLDLLNQDAN
Sbjct: 301 RT-PSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTG-VQRKLDLLNQDAN 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNSPN----NSIYKGSTMRGVSDTGEKLANMTIAARKPSIG 486
           KNDKIMKNTVRQQKYRPPVRNSPN    +SIYKGSTMRGVSDTGEKLANMTIAARKPSIG
Sbjct: 361 KNDKIMKNTVRQQKYRPPVRNSPNSKFHDSIYKGSTMRGVSDTGEKLANMTIAARKPSIG 420

Query: 487 QLPRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
           QLPRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG
Sbjct: 421 QLPRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 457

BLAST of MS021063 vs. NCBI nr
Match: XP_038904805.1 (cyclin-dependent kinase F-4 [Benincasa hispida] >XP_038904806.1 cyclin-dependent kinase F-4 [Benincasa hispida])

HSP 1 Score: 847.0 bits (2187), Expect = 8.4e-242
Identity = 417/455 (91.65%), Postives = 433/455 (95.16%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP
Sbjct: 1   MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSE EVRNWCFQVFQGLAYMHQRG
Sbjct: 61  NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSEAEVRNWCFQVFQGLAYMHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLVAK+LIKLADFGLARE SAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV
Sbjct: 121 YFHRDLKPENLLVAKDLIKLADFGLARETSAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAELFTLRPLFPGASE DQIYKIC+ILGTPTMD+WS GL LARNI+Y+FPQF
Sbjct: 181 DMWAMGAIMAELFTLRPLFPGASETDQIYKICNILGTPTMDTWSGGLCLARNINYQFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
            GVHLSVVIP+ASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRAR P +
Sbjct: 241 NGVHLSVVIPSASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARAPFA 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RTPPSAGTKD LEQQTAKK+P  LSDSR+AG+FS+PK P ALL TG VQRKLDL+NQDAN
Sbjct: 301 RTPPSAGTKDLLEQQTAKKYPVALSDSRIAGSFSNPKLP-ALLSTG-VQRKLDLMNQDAN 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR 486
           KNDK MK+TVRQQKYRPPVRNSP NSIYKG+ +RGVSDTGEKLANMTIA RKPSIGQLPR
Sbjct: 361 KNDKTMKSTVRQQKYRPPVRNSPTNSIYKGNNVRGVSDTGEKLANMTIAPRKPSIGQLPR 420

Query: 487 PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
           PMKAGVRWSGSSSDLL+RPAQE Q GSNY RKVAG
Sbjct: 421 PMKAGVRWSGSSSDLLIRPAQEFQPGSNYPRKVAG 453

BLAST of MS021063 vs. NCBI nr
Match: XP_008448596.1 (PREDICTED: cyclin-dependent kinase F-4-like isoform X1 [Cucumis melo] >KAA0052994.1 cyclin-dependent kinase F-4-like isoform X1 [Cucumis melo var. makuwa] >TYK11450.1 cyclin-dependent kinase F-4-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 845.5 bits (2183), Expect = 2.4e-241
Identity = 415/455 (91.21%), Postives = 433/455 (95.16%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP
Sbjct: 1   MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSE EVRNWCFQVFQGLAYMHQRG
Sbjct: 61  NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSEAEVRNWCFQVFQGLAYMHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLVAK+LIKLADFGLARE SA+PPYTEYVSTRWYRAPEVLLQSYLYGPKV
Sbjct: 121 YFHRDLKPENLLVAKDLIKLADFGLARETSAIPPYTEYVSTRWYRAPEVLLQSYLYGPKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAELFTLRPLFPGASE DQIYKIC+ILGTPTMD+WS GL LARNI+Y+FPQF
Sbjct: 181 DMWAMGAIMAELFTLRPLFPGASETDQIYKICNILGTPTMDTWSGGLCLARNINYQFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
            GVHLSVVIP+ASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRAR PI+
Sbjct: 241 NGVHLSVVIPSASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARAPIT 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RTPPSAGTKD LEQQTAKK+P  LSDSR+AGNFSSPK P A+L TG VQRKLDL+NQD N
Sbjct: 301 RTPPSAGTKDLLEQQTAKKYPVALSDSRIAGNFSSPKLP-AMLSTG-VQRKLDLMNQDLN 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR 486
           KNDK MK+TVRQQKYRPPVRNSP NSIYKG+ +RGVSDTGEKLANMTIA RKP+IGQLPR
Sbjct: 361 KNDKTMKSTVRQQKYRPPVRNSPTNSIYKGNNVRGVSDTGEKLANMTIAPRKPTIGQLPR 420

Query: 487 PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
           PMKAGVRWSGSSSDLL+RPAQE Q G+NY RKVAG
Sbjct: 421 PMKAGVRWSGSSSDLLIRPAQEFQSGNNYPRKVAG 453

BLAST of MS021063 vs. NCBI nr
Match: XP_004146120.2 (cyclin-dependent kinase F-4 [Cucumis sativus] >XP_031737775.1 cyclin-dependent kinase F-4 [Cucumis sativus] >KAE8650014.1 hypothetical protein Csa_010499 [Cucumis sativus])

HSP 1 Score: 842.4 bits (2175), Expect = 2.1e-240
Identity = 415/455 (91.21%), Postives = 431/455 (94.73%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP
Sbjct: 1   MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSE EVRNWCFQVFQGLAYMHQRG
Sbjct: 61  NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSEAEVRNWCFQVFQGLAYMHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLVAK+LIKLADFGLARE SAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV
Sbjct: 121 YFHRDLKPENLLVAKDLIKLADFGLARETSAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAELFTLRPLFPGASE DQIYKIC+ILGTPTMD+WS GL LARNI+Y+FPQF
Sbjct: 181 DMWAMGAIMAELFTLRPLFPGASETDQIYKICNILGTPTMDTWSGGLCLARNINYQFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
            GVHLSVVIP+ASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRAR PI+
Sbjct: 241 NGVHLSVVIPSASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARPPIT 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RTPPSAGTKD LEQQTAKK+P  LSDSR+ GNFSSPK P ALL TG VQRKLDL+NQD N
Sbjct: 301 RTPPSAGTKDVLEQQTAKKYPVALSDSRIGGNFSSPKLP-ALLSTG-VQRKLDLMNQDLN 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR 486
           KNDK MK+TVRQQKYRPPVRNSP NSIYKG+ +RGVSDTGEKLANMTIA RKP+IGQLPR
Sbjct: 361 KNDKTMKSTVRQQKYRPPVRNSPTNSIYKGNNVRGVSDTGEKLANMTIAPRKPTIGQLPR 420

Query: 487 PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
           PMKAGVRWS SSSDLL+RPAQE Q G+NY RKVAG
Sbjct: 421 PMKAGVRWSSSSSDLLIRPAQEFQTGNNYPRKVAG 453

BLAST of MS021063 vs. ExPASy Swiss-Prot
Match: Q6Z8C8 (Cyclin-dependent kinase F-4 OS=Oryza sativa subsp. japonica OX=39947 GN=CDKF-4 PE=2 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.1e-142
Identity = 266/465 (57.20%), Postives = 334/465 (71.83%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           M+R+ +IKEVGDGTFGSVWRAINKQ GEVVA+KKMK+KYY++EEC++LREVKSLR+MNHP
Sbjct: 1   MDRFKMIKEVGDGTFGSVWRAINKQNGEVVAVKKMKRKYYSFEECMSLREVKSLRRMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIREN+ILYF+ EYMECNLYQLMKD+ K FSE EVRNWCFQ+FQ LAYMHQRG
Sbjct: 61  NIVKLKEVIRENDILYFIMEYMECNLYQLMKDRVKPFSEAEVRNWCFQIFQALAYMHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLV+K++IKLADFGLARE++++PPYTEYVSTRWYRAPEVLLQS +Y   V
Sbjct: 121 YFHRDLKPENLLVSKDVIKLADFGLAREVTSVPPYTEYVSTRWYRAPEVLLQSSIYDSAV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAEL TL PLFPG SE D+I KIC+++G+P   SW  GLSLA  + ++FPQ 
Sbjct: 181 DMWAMGAIMAELLTLHPLFPGTSEADEILKICNVIGSPDEQSWPQGLSLAETMKFQFPQV 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPI- 366
            G  L+ V+ + S +AV+LI+SLCSWDP KRP A E LQH FFQ C +VPP++R++  + 
Sbjct: 241 SGNQLAEVMTSVSSEAVDLISSLCSWDPCKRPKAAEVLQHTFFQGCTFVPPTVRSKAGVL 300

Query: 367 SRTPPSAGTKDFLEQQTAKKF-PGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQD 426
            +TPP  G K   E    +++  GTLS ++   N +S K  G  L    VQRKL +  Q 
Sbjct: 301 PKTPPCVGVKGVSEHGMPRRYSTGTLSTTKPHSN-ASLKSSG--LSKTGVQRKLQMDRQA 360

Query: 427 ANKNDKIMKNTVRQQKYRPPVRNSPNNSIYKGS---------TMRGVSDTGEKLANMTIA 486
             K  K  ++  +    R P RNSP + + + S         TM  VS   E+L +M++ 
Sbjct: 361 PQKIKKPTESNNKLTTNRVPARNSPGHPVLRHSRSLPETGRATMHKVSTLTERLTHMSVT 420

Query: 487 ARKPSIGQLPRP-MKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKV 520
           +R  +  +   P +KAG+      SDLL +   EI      +RK+
Sbjct: 421 SRTRTTPKPAAPLLKAGL----GKSDLLGK-TDEIPPAKRLTRKL 457

BLAST of MS021063 vs. ExPASy Swiss-Prot
Match: Q84SN3 (Cyclin-dependent kinase F-3 OS=Oryza sativa subsp. japonica OX=39947 GN=CDKF-3 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 5.1e-109
Identity = 182/295 (61.69%), Postives = 235/295 (79.66%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERY +I+E+GDGT G+V+RA N +T E+VA+KKMK+K++ WEEC++LREVK+L+K+NHP
Sbjct: 1   MERYKVIREIGDGTCGNVFRAYNTETNEIVAVKKMKRKFFQWEECISLREVKALQKLNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEV  EN+ L+F+FE MECNLY ++++++  FSE E+RN+  Q+ QGLAYMH  G
Sbjct: 61  NIVKLKEVTMENHELFFIFENMECNLYDVIRERQAAFSEEEIRNFMVQILQGLAYMHNNG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLV    +K+ADFGLARE+S+ PPYT+YVSTRWYRAPEVLLQS  Y P +
Sbjct: 121 YFHRDLKPENLLVTDGTVKIADFGLAREVSSSPPYTDYVSTRWYRAPEVLLQSSAYTPAI 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWA+GAI+AELFTL PLFPG SE DQ+YKIC++LGTP    W  G++L R+ S+ F Q 
Sbjct: 181 DMWAVGAILAELFTLSPLFPGGSETDQLYKICAVLGTPDHTVWPEGMNLPRSSSFNFFQI 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRA 362
              +L  +IP A+ +A++LI  LCSWDP +RPTA ++LQHPFF    +VP  L A
Sbjct: 241 PPRNLWELIPNATLEAIDLIQQLCSWDPRRRPTAEQSLQHPFFNVGNWVPRPLHA 295

BLAST of MS021063 vs. ExPASy Swiss-Prot
Match: P43294 (Serine/threonine-protein kinase MHK OS=Arabidopsis thaliana OX=3702 GN=MHK PE=2 SV=2)

HSP 1 Score: 384.4 bits (986), Expect = 2.0e-105
Identity = 176/283 (62.19%), Postives = 232/283 (81.98%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           M RY +++E+GDGT GSV++A+N +T EVVA+KKMK+K+Y WEECVNLREVK+LRK+NHP
Sbjct: 9   MCRYKILEELGDGTCGSVYKAVNLETYEVVAVKKMKRKFYYWEECVNLREVKALRKLNHP 68

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           +I+KLKE++RE+N L+F+FE M+ NLY +MK++E+ FSE E+R++  Q+ QGLA+MH+ G
Sbjct: 69  HIIKLKEIVREHNELFFIFECMDHNLYHIMKERERPFSEGEIRSFMSQMLQGLAHMHKNG 128

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLV   ++K+ADFGLARE+++MPPYTEYVSTRWYRAPEVLLQS LY P V
Sbjct: 129 YFHRDLKPENLLVTNNILKIADFGLAREVASMPPYTEYVSTRWYRAPEVLLQSSLYTPAV 188

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWA+GAI+AEL+ L PLFPG SEIDQ+YKIC +LG P   ++    S++R +S    +F
Sbjct: 189 DMWAVGAILAELYALTPLFPGESEIDQLYKICCVLGKPDWTTFPEAKSISRIMSISHTEF 248

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFF 350
               ++ ++P A+ +A++LI  LCSWDP KRPTA EAL HPFF
Sbjct: 249 PQTRIADLLPNAAPEAIDLINRLCSWDPLKRPTADEALNHPFF 291

BLAST of MS021063 vs. ExPASy Swiss-Prot
Match: Q04859 (Serine/threonine-protein kinase MAK OS=Mus musculus OX=10090 GN=Mak PE=1 SV=2)

HSP 1 Score: 383.6 bits (984), Expect = 3.4e-105
Identity = 170/285 (59.65%), Postives = 232/285 (81.40%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           M RY  +K++GDGT+GSV    + ++GE+VAIK+MK+K+Y+W+EC+NLREVKSL+K+NH 
Sbjct: 1   MNRYTTMKQLGDGTYGSVLMGKSNESGELVAIKRMKRKFYSWDECMNLREVKSLKKLNHA 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           N++KLKEVIREN+ LYFVFEYM+ NLYQLMKD+ KLF E+ +RN  +Q+ QGLA++H+ G
Sbjct: 61  NVIKLKEVIRENDHLYFVFEYMKENLYQLMKDRNKLFPESVIRNIMYQILQGLAFIHKHG 120

Query: 187 YFHRDLKPENLL-VAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPK 246
           +FHRD+KPENLL +  EL+K+ADFGLARE+ + PPYT+YVSTRWYRAPEVLL+S +Y   
Sbjct: 121 FFHRDMKPENLLCMGPELVKIADFGLARELRSQPPYTDYVSTRWYRAPEVLLRSSVYSSP 180

Query: 247 VDMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQ 306
           +D+WA+G+IMAEL+T RPLFPG SE+D+I+KIC +LGTP    W  G  LA ++++ FPQ
Sbjct: 181 IDVWAVGSIMAELYTFRPLFPGTSEVDEIFKICQVLGTPKKSDWPEGYQLASSMNFRFPQ 240

Query: 307 FGGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQ 351
              ++L  +IP AS +A+ L+  + +WDP KRPTA +AL+HP+FQ
Sbjct: 241 CIPINLKTLIPNASSEAIQLMTEMLNWDPKKRPTASQALKHPYFQ 285

BLAST of MS021063 vs. ExPASy Swiss-Prot
Match: P20793 (Serine/threonine-protein kinase MAK OS=Rattus norvegicus OX=10116 GN=Mak PE=1 SV=2)

HSP 1 Score: 382.9 bits (982), Expect = 5.9e-105
Identity = 170/293 (58.02%), Postives = 235/293 (80.20%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           M RY  ++++GDGT+GSV    + ++GE+VAIK+MK+K+Y+W+EC+NLREVKSL+K+NH 
Sbjct: 1   MNRYTTMRQLGDGTYGSVLMGKSNESGELVAIKRMKRKFYSWDECMNLREVKSLKKLNHA 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           N++KLKEVIREN+ LYF+FEYM+ NLYQLMKD+ KLF E+ +RN  +Q+ QGLA++H+ G
Sbjct: 61  NVIKLKEVIRENDHLYFIFEYMKENLYQLMKDRNKLFPESVIRNIMYQILQGLAFIHKHG 120

Query: 187 YFHRDLKPENLL-VAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPK 246
           +FHRD+KPENLL +  EL+K+ADFGLARE+ + PPYT+YVSTRWYRAPEVLL+S +Y   
Sbjct: 121 FFHRDMKPENLLCMGPELVKIADFGLARELRSQPPYTDYVSTRWYRAPEVLLRSSVYSSP 180

Query: 247 VDMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQ 306
           +D+WA+G+IMAEL+T RPLFPG SE+D+I+KIC +LGTP    W  G  LA ++++ FPQ
Sbjct: 181 IDVWAVGSIMAELYTFRPLFPGTSEVDEIFKICQVLGTPKKSDWPEGYQLASSMNFRFPQ 240

Query: 307 FGGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPS 359
              ++L  +IP AS +A+ L+  + +WDP KRPTA +AL+HP+FQ    + PS
Sbjct: 241 CIPINLKTLIPNASSEAIQLMTEMLNWDPKKRPTASQALKHPYFQVGQVLGPS 293

BLAST of MS021063 vs. ExPASy TrEMBL
Match: A0A0A0L148 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G005580 PE=4 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.0e-269
Identity = 468/522 (89.66%), Postives = 487/522 (93.30%), Query Frame = 0

Query: 1   MISTGGVMKLFCEIGVGSLVQLFKALLMHHPDDFLGETSAGINASYQFK-SLSLLNKGGC 60
           MIST GVMKL  +IGVGSLVQLFKALLM H DDFLGETS GINA+Y F+ SL LLNKGGC
Sbjct: 1   MISTRGVMKLLYKIGVGSLVQLFKALLMQHRDDFLGETSLGINANYPFEVSLLLLNKGGC 60

Query: 61  LCASARKMERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKS 120
           LCA  RKMERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKS
Sbjct: 61  LCAPTRKMERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKS 120

Query: 121 LRKMNHPNIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGL 180
           LRKMNHPNIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSE EVRNWCFQVFQGL
Sbjct: 121 LRKMNHPNIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSEAEVRNWCFQVFQGL 180

Query: 181 AYMHQRGYFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQS 240
           AYMHQRGYFHRDLKPENLLVAK+LIKLADFGLARE SAMPPYTEYVSTRWYRAPEVLLQS
Sbjct: 181 AYMHQRGYFHRDLKPENLLVAKDLIKLADFGLARETSAMPPYTEYVSTRWYRAPEVLLQS 240

Query: 241 YLYGPKVDMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNI 300
           YLYGPKVDMWAMGAIMAELFTLRPLFPGASE DQIYKIC+ILGTPTMD+WS GL LARNI
Sbjct: 241 YLYGPKVDMWAMGAIMAELFTLRPLFPGASETDQIYKICNILGTPTMDTWSGGLCLARNI 300

Query: 301 SYEFPQFGGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSL 360
           +Y+FPQF GVHLSVVIP+ASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSL
Sbjct: 301 NYQFPQFNGVHLSVVIPSASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSL 360

Query: 361 RARVPISRTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLD 420
           RAR PI+RTPPSAGTKD LEQQTAKK+P  LSDSR+ GNFSSPK P ALL TG VQRKLD
Sbjct: 361 RARPPITRTPPSAGTKDVLEQQTAKKYPVALSDSRIGGNFSSPKLP-ALLSTG-VQRKLD 420

Query: 421 LLNQDANKNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKP 480
           L+NQD NKNDK MK+TVRQQKYRPPVRNSP NSIYKG+ +RGVSDTGEKLANMTIA RKP
Sbjct: 421 LMNQDLNKNDKTMKSTVRQQKYRPPVRNSPTNSIYKGNNVRGVSDTGEKLANMTIAPRKP 480

Query: 481 SIGQLPRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
           +IGQLPRPMKAGVRWS SSSDLL+RPAQE Q G+NY RKVAG
Sbjct: 481 TIGQLPRPMKAGVRWSSSSSDLLIRPAQEFQTGNNYPRKVAG 520

BLAST of MS021063 vs. ExPASy TrEMBL
Match: A0A6J1CV61 (cyclin-dependent kinase F-4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014864 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.1e-259
Identity = 451/455 (99.12%), Postives = 452/455 (99.34%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP
Sbjct: 1   MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFS TEVRNWCFQVFQGLAY+HQRG
Sbjct: 61  NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSVTEVRNWCFQVFQGLAYIHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV
Sbjct: 121 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF
Sbjct: 181 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
           GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS
Sbjct: 241 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RT PSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTG VQRKLDLLNQDAN
Sbjct: 301 RT-PSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTG-VQRKLDLLNQDAN 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR 486
           KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR
Sbjct: 361 KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR 420

Query: 487 PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
           PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG
Sbjct: 421 PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 453

BLAST of MS021063 vs. ExPASy TrEMBL
Match: A0A6J1CVV5 (cyclin-dependent kinase F-4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014864 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.5e-257
Identity = 450/459 (98.04%), Postives = 452/459 (98.47%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP
Sbjct: 1   MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFS TEVRNWCFQVFQGLAY+HQRG
Sbjct: 61  NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSVTEVRNWCFQVFQGLAYIHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV
Sbjct: 121 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF
Sbjct: 181 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
           GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS
Sbjct: 241 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RT PSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTG VQRKLDLLNQDAN
Sbjct: 301 RT-PSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTG-VQRKLDLLNQDAN 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNSPN----NSIYKGSTMRGVSDTGEKLANMTIAARKPSIG 486
           KNDKIMKNTVRQQKYRPPVRNSPN    +SIYKGSTMRGVSDTGEKLANMTIAARKPSIG
Sbjct: 361 KNDKIMKNTVRQQKYRPPVRNSPNSKFHDSIYKGSTMRGVSDTGEKLANMTIAARKPSIG 420

Query: 487 QLPRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
           QLPRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG
Sbjct: 421 QLPRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 457

BLAST of MS021063 vs. ExPASy TrEMBL
Match: A0A5A7UCL7 (Cyclin-dependent kinase F-4-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001070 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.2e-241
Identity = 415/455 (91.21%), Postives = 433/455 (95.16%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP
Sbjct: 1   MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSE EVRNWCFQVFQGLAYMHQRG
Sbjct: 61  NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSEAEVRNWCFQVFQGLAYMHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLVAK+LIKLADFGLARE SA+PPYTEYVSTRWYRAPEVLLQSYLYGPKV
Sbjct: 121 YFHRDLKPENLLVAKDLIKLADFGLARETSAIPPYTEYVSTRWYRAPEVLLQSYLYGPKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAELFTLRPLFPGASE DQIYKIC+ILGTPTMD+WS GL LARNI+Y+FPQF
Sbjct: 181 DMWAMGAIMAELFTLRPLFPGASETDQIYKICNILGTPTMDTWSGGLCLARNINYQFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
            GVHLSVVIP+ASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRAR PI+
Sbjct: 241 NGVHLSVVIPSASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARAPIT 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RTPPSAGTKD LEQQTAKK+P  LSDSR+AGNFSSPK P A+L TG VQRKLDL+NQD N
Sbjct: 301 RTPPSAGTKDLLEQQTAKKYPVALSDSRIAGNFSSPKLP-AMLSTG-VQRKLDLMNQDLN 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR 486
           KNDK MK+TVRQQKYRPPVRNSP NSIYKG+ +RGVSDTGEKLANMTIA RKP+IGQLPR
Sbjct: 361 KNDKTMKSTVRQQKYRPPVRNSPTNSIYKGNNVRGVSDTGEKLANMTIAPRKPTIGQLPR 420

Query: 487 PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
           PMKAGVRWSGSSSDLL+RPAQE Q G+NY RKVAG
Sbjct: 421 PMKAGVRWSGSSSDLLIRPAQEFQSGNNYPRKVAG 453

BLAST of MS021063 vs. ExPASy TrEMBL
Match: A0A1S3BK18 (cyclin-dependent kinase F-4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490725 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.2e-241
Identity = 415/455 (91.21%), Postives = 433/455 (95.16%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP
Sbjct: 1   MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSE EVRNWCFQVFQGLAYMHQRG
Sbjct: 61  NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSEAEVRNWCFQVFQGLAYMHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLVAK+LIKLADFGLARE SA+PPYTEYVSTRWYRAPEVLLQSYLYGPKV
Sbjct: 121 YFHRDLKPENLLVAKDLIKLADFGLARETSAIPPYTEYVSTRWYRAPEVLLQSYLYGPKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAELFTLRPLFPGASE DQIYKIC+ILGTPTMD+WS GL LARNI+Y+FPQF
Sbjct: 181 DMWAMGAIMAELFTLRPLFPGASETDQIYKICNILGTPTMDTWSGGLCLARNINYQFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
            GVHLSVVIP+ASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRAR PI+
Sbjct: 241 NGVHLSVVIPSASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARAPIT 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RTPPSAGTKD LEQQTAKK+P  LSDSR+AGNFSSPK P A+L TG VQRKLDL+NQD N
Sbjct: 301 RTPPSAGTKDLLEQQTAKKYPVALSDSRIAGNFSSPKLP-AMLSTG-VQRKLDLMNQDLN 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPR 486
           KNDK MK+TVRQQKYRPPVRNSP NSIYKG+ +RGVSDTGEKLANMTIA RKP+IGQLPR
Sbjct: 361 KNDKTMKSTVRQQKYRPPVRNSPTNSIYKGNNVRGVSDTGEKLANMTIAPRKPTIGQLPR 420

Query: 487 PMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
           PMKAGVRWSGSSSDLL+RPAQE Q G+NY RKVAG
Sbjct: 421 PMKAGVRWSGSSSDLLIRPAQEFQSGNNYPRKVAG 453

BLAST of MS021063 vs. TAIR 10
Match: AT4G19110.2 (Protein kinase superfamily protein )

HSP 1 Score: 625.5 bits (1612), Expect = 3.7e-179
Identity = 308/470 (65.53%), Postives = 379/470 (80.64%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           M+RY LIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYY+W+EC+NLREVKSLR+MNHP
Sbjct: 1   MDRYKLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYSWDECINLREVKSLRRMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIREN+ILYFVFEYMECNLYQLMKD++KLF+E +++NWCFQVFQGL+YMHQRG
Sbjct: 61  NIVKLKEVIRENDILYFVFEYMECNLYQLMKDRQKLFAEADIKNWCFQVFQGLSYMHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLV+K++IK+ADFGLARE+++ PP+TEYVSTRWYRAPEVLLQSY+Y  KV
Sbjct: 121 YFHRDLKPENLLVSKDIIKIADFGLAREVNSSPPFTEYVSTRWYRAPEVLLQSYVYTSKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAEL +LRP+FPGASE D+IYKICS++GTPT ++W  GL+LA  I+Y+FPQ 
Sbjct: 181 DMWAMGAIMAELLSLRPIFPGASEADEIYKICSVIGTPTEETWLEGLNLANTINYQFPQL 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
            GV LS ++P+AS+DA+NLI  LCSWDPS RPTA E LQHPFFQSC+YVPPSLR +  ++
Sbjct: 241 PGVPLSSLMPSASEDAINLIERLCSWDPSSRPTAAEVLQHPFFQSCFYVPPSLRPKPSVA 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RTPP  G +   E Q+ K++P +L++++   ++ SPK   A  G+G VQRKLD++NQD  
Sbjct: 301 RTPPPVGPRGSFEHQSVKRYPVSLANAKPFNSYVSPKS-NAAFGSG-VQRKLDMVNQDGT 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNSPNN----SIYKGSTMR-GVSDTGEKLANMTIAA---RK 486
           +N K ++++VR  KYRPP + SP+N    S+ K    R  VS+T +KLANMTI A   R+
Sbjct: 361 RNTKPVRSSVRDSKYRPPGKKSPHNAAAASLNKNRVTRSSVSETADKLANMTIGATGSRR 420

Query: 487 PSIGQL-------PRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
            S+  +       P PMKAG  W G + D+ LRP Q   +   YSRKVAG
Sbjct: 421 HSVSVVGQHQQLKPPPMKAG--WVGETRDMFLRPTQPTTNA--YSRKVAG 464

BLAST of MS021063 vs. TAIR 10
Match: AT4G19110.1 (Protein kinase superfamily protein )

HSP 1 Score: 622.5 bits (1604), Expect = 3.1e-178
Identity = 307/467 (65.74%), Postives = 377/467 (80.73%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           M+RY LIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYY+W+EC+NLREVKSLR+MNHP
Sbjct: 1   MDRYKLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYSWDECINLREVKSLRRMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIREN+ILYFVFEYMECNLYQLMKD++KLF+E +++NWCFQVFQGL+YMHQRG
Sbjct: 61  NIVKLKEVIRENDILYFVFEYMECNLYQLMKDRQKLFAEADIKNWCFQVFQGLSYMHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLV+K++IK+ADFGLARE+++ PP+TEYVSTRWYRAPEVLLQSY+Y  KV
Sbjct: 121 YFHRDLKPENLLVSKDIIKIADFGLAREVNSSPPFTEYVSTRWYRAPEVLLQSYVYTSKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAEL +LRP+FPGASE D+IYKICS++GTPT ++W  GL+LA  I+Y+FPQ 
Sbjct: 181 DMWAMGAIMAELLSLRPIFPGASEADEIYKICSVIGTPTEETWLEGLNLANTINYQFPQL 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARVPIS 366
            GV LS ++P+AS+DA+NLI  LCSWDPS RPTA E LQHPFFQSC+YVPPSLR +  ++
Sbjct: 241 PGVPLSSLMPSASEDAINLIERLCSWDPSSRPTAAEVLQHPFFQSCFYVPPSLRPKPSVA 300

Query: 367 RTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLNQDAN 426
           RTPP  G +   E Q+ K++P +L++++   ++ SPK   A  G+G VQRKLD++NQD  
Sbjct: 301 RTPPPVGPRGSFEHQSVKRYPVSLANAKPFNSYVSPKS-NAAFGSG-VQRKLDMVNQDGT 360

Query: 427 KNDKIMKNTVRQQKYRPPVRNS-PNNSIYKGSTMR-GVSDTGEKLANMTIAA---RKPSI 486
           +N K ++++VR  KYRPP + S P  S+ K    R  VS+T +KLANMTI A   R+ S+
Sbjct: 361 RNTKPVRSSVRDSKYRPPGKKSPPAASLNKNRVTRSSVSETADKLANMTIGATGSRRHSV 420

Query: 487 GQL-------PRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
             +       P PMKAG  W G + D+ LRP Q   +   YSRKVAG
Sbjct: 421 SVVGQHQQLKPPPMKAG--WVGETRDMFLRPTQPTTNA--YSRKVAG 461

BLAST of MS021063 vs. TAIR 10
Match: AT5G45430.2 (Protein kinase superfamily protein )

HSP 1 Score: 558.1 bits (1437), Expect = 7.3e-159
Identity = 292/503 (58.05%), Postives = 347/503 (68.99%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERY L+KEVGDGTFG+VWRA+NKQT EVVAIK+MKKKY++WEECVNLREVKSL +MNHP
Sbjct: 1   MERYTLLKEVGDGTFGNVWRAVNKQTNEVVAIKRMKKKYFSWEECVNLREVKSLSRMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIREN+ILYFVFEYMECNLYQLMKD+ K F+E+++RNWCFQVFQGL+YMHQRG
Sbjct: 61  NIVKLKEVIRENDILYFVFEYMECNLYQLMKDRPKHFAESDIRNWCFQVFQGLSYMHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLV+K++IK+AD GLAREI + PPYTEYVSTRWYRAPEVLLQSY+Y  KV
Sbjct: 121 YFHRDLKPENLLVSKDVIKIADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAEL +LRPLFPGASE D+IYKICS++G+PT ++W  GL+LA  I+Y+FPQF
Sbjct: 181 DMWAMGAIMAELLSLRPLFPGASEADEIYKICSVIGSPTEETWLEGLNLASVINYQFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARV--- 366
            GVHLS V+P AS DAVNLI  LCSWDP  RPT  EALQHPFFQSCYY+PPSLR ++   
Sbjct: 241 PGVHLSSVMPYASADAVNLIERLCSWDPCNRPTTAEALQHPFFQSCYYIPPSLRPKLSEP 300

Query: 367 ------------------------------PISRTPPSAGTKDFLEQQTAKKFPGTLSD- 426
                                         P +  P S G   ++  +T   F   ++  
Sbjct: 301 RGSLEQQQSVKRLPAAPTYNANKPLNTYVTPKTNAPFSNGVNSYVTPKTNAPFSNGVNSY 360

Query: 427 ---------SRVAGNFSSPKQPGALLGTGSVQRKLDLLNQD----ANKNDKIMKNTVRQQ 486
                    S    N+ +PK   A    G+ Q+K+D  N +    A  N  +    V+  
Sbjct: 361 VTAKTNAPFSNGVNNYVAPKM-NAPFDNGATQQKVDTTNNNNQNTAWNNKPVRSYNVKDS 420

Query: 487 KYRPPVRNSPNNSIYKGSTMRGVSDTGEKLANMTIAARKPSIGQLPRP-MKAGVRWSGSS 522
           KYRPP R SP +        RG S+T  KLAN TI  R     +  +P MKAG  W G S
Sbjct: 421 KYRPPGRKSPPSMEKNWVFSRGASETANKLANATIGGRWTQSQRQQQPAMKAG--WVGES 480

BLAST of MS021063 vs. TAIR 10
Match: AT5G45430.1 (Protein kinase superfamily protein )

HSP 1 Score: 555.4 bits (1430), Expect = 4.7e-158
Identity = 292/505 (57.82%), Postives = 350/505 (69.31%), Query Frame = 0

Query: 67  MERYMLIKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYTWEECVNLREVKSLRKMNHP 126
           MERY L+KEVGDGTFG+VWRA+NKQT EVVAIK+MKKKY++WEECVNLREVKSL +MNHP
Sbjct: 1   MERYTLLKEVGDGTFGNVWRAVNKQTNEVVAIKRMKKKYFSWEECVNLREVKSLSRMNHP 60

Query: 127 NIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYMHQRG 186
           NIVKLKEVIREN+ILYFVFEYMECNLYQLMKD+ K F+E+++RNWCFQVFQGL+YMHQRG
Sbjct: 61  NIVKLKEVIRENDILYFVFEYMECNLYQLMKDRPKHFAESDIRNWCFQVFQGLSYMHQRG 120

Query: 187 YFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLYGPKV 246
           YFHRDLKPENLLV+K++IK+AD GLAREI + PPYTEYVSTRWYRAPEVLLQSY+Y  KV
Sbjct: 121 YFHRDLKPENLLVSKDVIKIADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKV 180

Query: 247 DMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYEFPQF 306
           DMWAMGAIMAEL +LRPLFPGASE D+IYKICS++G+PT ++W  GL+LA  I+Y+FPQF
Sbjct: 181 DMWAMGAIMAELLSLRPLFPGASEADEIYKICSVIGSPTEETWLEGLNLASVINYQFPQF 240

Query: 307 GGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRARV--- 366
            GVHLS V+P AS DAVNLI  LCSWDP  RPT  EALQHPFFQSCYY+PPSLR ++   
Sbjct: 241 PGVHLSSVMPYASADAVNLIERLCSWDPCNRPTTAEALQHPFFQSCYYIPPSLRPKLSEP 300

Query: 367 ------------------------------PISRTPPSAGTKDFLEQQTAKKFPGTLSD- 426
                                         P +  P S G   ++  +T   F   ++  
Sbjct: 301 RGSLEQQQSVKRLPAAPTYNANKPLNTYVTPKTNAPFSNGVNSYVTPKTNAPFSNGVNSY 360

Query: 427 ---------SRVAGNFSSPKQPGALLGTGSVQRKLDLLNQD----ANKNDKIMKNTVRQQ 486
                    S    N+ +PK   A    G+ Q+K+D  N +    A  N  +    V+  
Sbjct: 361 VTAKTNAPFSNGVNNYVAPKM-NAPFDNGATQQKVDTTNNNNQNTAWNNKPVRSYNVKDS 420

Query: 487 KYRPPVRNSPNNSIYKGSTM--RGVSDTGEKLANMTIAARKPSIGQLPRP-MKAGVRWSG 522
           KYRPP R SP  +  + + +  RG S+T  KLAN TI  R     +  +P MKAG  W G
Sbjct: 421 KYRPPGRKSPLAASMEKNWVFSRGASETANKLANATIGGRWTQSQRQQQPAMKAG--WVG 480

BLAST of MS021063 vs. TAIR 10
Match: AT4G19110.3 (Protein kinase superfamily protein )

HSP 1 Score: 522.3 bits (1344), Expect = 4.4e-148
Identity = 257/410 (62.68%), Postives = 322/410 (78.54%), Query Frame = 0

Query: 123 MNHPNIVKLKEVIRENNILYFVFEYMECNLYQLMKDKEKLFSETEVRNWCFQVFQGLAYM 182
           MNHPNIVKLKEVIREN+ILYFVFEYMECNLYQLMKD++KLF+E +++NWCFQVFQGL+YM
Sbjct: 1   MNHPNIVKLKEVIRENDILYFVFEYMECNLYQLMKDRQKLFAEADIKNWCFQVFQGLSYM 60

Query: 183 HQRGYFHRDLKPENLLVAKELIKLADFGLAREISAMPPYTEYVSTRWYRAPEVLLQSYLY 242
           HQRGYFHRDLKPENLLV+K++IK+ADFGLARE+++ PP+TEYVSTRWYRAPEVLLQSY+Y
Sbjct: 61  HQRGYFHRDLKPENLLVSKDIIKIADFGLAREVNSSPPFTEYVSTRWYRAPEVLLQSYVY 120

Query: 243 GPKVDMWAMGAIMAELFTLRPLFPGASEIDQIYKICSILGTPTMDSWSAGLSLARNISYE 302
             KVDMWAMGAIMAEL +LRP+FPGASE D+IYKICS++GTPT ++W  GL+LA  I+Y+
Sbjct: 121 TSKVDMWAMGAIMAELLSLRPIFPGASEADEIYKICSVIGTPTEETWLEGLNLANTINYQ 180

Query: 303 FPQFGGVHLSVVIPAASDDAVNLIASLCSWDPSKRPTAMEALQHPFFQSCYYVPPSLRAR 362
           FPQ  GV LS ++P+AS+DA+NLI  LCSWDPS RPTA E LQHPFFQSC+YVPPSLR +
Sbjct: 181 FPQLPGVPLSSLMPSASEDAINLIERLCSWDPSSRPTAAEVLQHPFFQSCFYVPPSLRPK 240

Query: 363 VPISRTPPSAGTKDFLEQQTAKKFPGTLSDSRVAGNFSSPKQPGALLGTGSVQRKLDLLN 422
             ++RTPP  G +   E Q+ K++P +L++++   ++ SPK   A  G+G VQRKLD++N
Sbjct: 241 PSVARTPPPVGPRGSFEHQSVKRYPVSLANAKPFNSYVSPKS-NAAFGSG-VQRKLDMVN 300

Query: 423 QDANKNDKIMKNTVRQQKYRPPVRNSPNNSIYKGSTMR-GVSDTGEKLANMTIAA---RK 482
           QD  +N K ++++VR  KYRPP + SP  S+ K    R  VS+T +KLANMTI A   R+
Sbjct: 301 QDGTRNTKPVRSSVRDSKYRPPGKKSPPASLNKNRVTRSSVSETADKLANMTIGATGSRR 360

Query: 483 PSIGQL-------PRPMKAGVRWSGSSSDLLLRPAQEIQHGSNYSRKVAG 522
            S+  +       P PMKAG  W G + D+ LRP Q   +   YSRKVAG
Sbjct: 361 HSVSVVGQHQQLKPPPMKAG--WVGETRDMFLRPTQPTTNA--YSRKVAG 404

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145414.14.4e-25999.12cyclin-dependent kinase F-4 isoform X2 [Momordica charantia][more]
XP_022145412.19.2e-25798.04cyclin-dependent kinase F-4 isoform X1 [Momordica charantia] >XP_022145413.1 cyc... [more]
XP_038904805.18.4e-24291.65cyclin-dependent kinase F-4 [Benincasa hispida] >XP_038904806.1 cyclin-dependent... [more]
XP_008448596.12.4e-24191.21PREDICTED: cyclin-dependent kinase F-4-like isoform X1 [Cucumis melo] >KAA005299... [more]
XP_004146120.22.1e-24091.21cyclin-dependent kinase F-4 [Cucumis sativus] >XP_031737775.1 cyclin-dependent k... [more]
Match NameE-valueIdentityDescription
Q6Z8C82.1e-14257.20Cyclin-dependent kinase F-4 OS=Oryza sativa subsp. japonica OX=39947 GN=CDKF-4 P... [more]
Q84SN35.1e-10961.69Cyclin-dependent kinase F-3 OS=Oryza sativa subsp. japonica OX=39947 GN=CDKF-3 P... [more]
P432942.0e-10562.19Serine/threonine-protein kinase MHK OS=Arabidopsis thaliana OX=3702 GN=MHK PE=2 ... [more]
Q048593.4e-10559.65Serine/threonine-protein kinase MAK OS=Mus musculus OX=10090 GN=Mak PE=1 SV=2[more]
P207935.9e-10558.02Serine/threonine-protein kinase MAK OS=Rattus norvegicus OX=10116 GN=Mak PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A0A0L1481.0e-26989.66Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G005... [more]
A0A6J1CV612.1e-25999.12cyclin-dependent kinase F-4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1CVV54.5e-25798.04cyclin-dependent kinase F-4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5A7UCL71.2e-24191.21Cyclin-dependent kinase F-4-like isoform X1 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BK181.2e-24191.21cyclin-dependent kinase F-4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490... [more]
Match NameE-valueIdentityDescription
AT4G19110.23.7e-17965.53Protein kinase superfamily protein [more]
AT4G19110.13.1e-17865.74Protein kinase superfamily protein [more]
AT5G45430.27.3e-15958.05Protein kinase superfamily protein [more]
AT5G45430.14.7e-15857.82Protein kinase superfamily protein [more]
AT4G19110.34.4e-14862.68Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 70..349
e-value: 6.4E-103
score: 357.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 70..349
e-value: 6.5E-76
score: 255.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 70..349
score: 48.682457
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 60..149
e-value: 1.5E-25
score: 90.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 150..356
e-value: 5.8E-66
score: 224.3
NoneNo IPR availablePIRSRPIRSR000556-1PIRSR000556-1coord: 59..261
e-value: 4.5E-19
score: 66.2
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 73..255
e-value: 4.1E-26
score: 89.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 441..462
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 441..459
NoneNo IPR availablePANTHERPTHR24055:SF472CYCLIN-DEPENDENT KINASE F-4-LIKE ISOFORM X1coord: 67..470
NoneNo IPR availablePANTHERPTHR24055MITOGEN-ACTIVATED PROTEIN KINASEcoord: 67..470
NoneNo IPR availableCDDcd07830STKc_MAK_likecoord: 70..349
e-value: 0.0
score: 532.88
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 76..100
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 187..199
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 65..354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS021063.1MS021063.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity