MS020983 (gene) Bitter gourd (TR) v1

BLAST of MS020983 vs. NCBI nr
Match: KAG6577804.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 712.2 bits (1837), Expect = 3.2e-201
Identity = 368/528 (69.70%), Postives = 426/528 (80.68%), Query Frame = 0

Query: 6   PANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRISPGDL 65
           PAN LPLSPVTF KRAAA+Y  R S+IYG+R FTW++TY R L LASAL+HHF ISPGD+
Sbjct: 8   PANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHISPGDV 67

Query: 66  VAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFLPILL 125
           VAAMAPN+PELYELH+AVPMAGAIISPLNTKLD+ TL+LLL QL PK+IF+DS FLP +L
Sbjct: 68  VAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVL 127

Query: 126 QSLSQNNANSIKPAAVVIVIPTGPPEVPS----YDGVLAMGSDDFTPCPNAELDPISVNC 185
           +SLSQ   NSI+  A+V+ IPT P   PS    Y+ VL M  DDFTP  NAELD IS+NC
Sbjct: 128 KSLSQTYGNSIQFPALVL-IPTHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELDAISINC 187

Query: 186 TSGSTGLHKCVVYSHRATYLNSLATILRTQICR-SAPPVFLWTVDMFRCNGWCFVWAVAA 245
           TSGSTGLHK VVYSHRA YLNSLATI R+ IC+ ++ PVFLWTVDMFRCNGWCF+WA+AA
Sbjct: 188 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 247

Query: 246 VGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAP--NRKPPPPLRRVDLIVA 305
           +GGCN+CLR VTA+AIF  +ELH VTL CGP T+LK+I E+   N   P   RRVDLIVA
Sbjct: 248 LGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVA 307

Query: 306 GALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGG-GGGVSLLEDLITSLEVDVK 365
           GALP+ EIL++VT+ GFNISYGYGMTEA+GP +IRP        +   EDL TSLE+DVK
Sbjct: 308 GALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEEIVQFEDLFTSLEIDVK 367

Query: 366 DSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGDWYRTGDVAVRHRSGRIE 425
           D  SMESV  DGETLGE+MLRGNTLMSGY++N KAT+ AFSGDWYRTGDV VRH+SGRIE
Sbjct: 368 DPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRHKSGRIE 427

Query: 426 MKDRANDVIVTADG-AAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIRPCGFVKLRNG 485
           MKDRA D++V A+G AAVSTV+VEAVL+SHP VAEAAVVG+        R CGFVKL+NG
Sbjct: 428 MKDRAKDIVVRANGEAAVSTVQVEAVLMSHPSVAEAAVVGE--------RLCGFVKLKNG 487

Query: 486 CRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNVK 525
            RASAEEIV FC  HLPEFM+P  +VFGDLP+NS GKVQKFI+R+  K
Sbjct: 488 SRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAK 526

BLAST of MS020983 vs. NCBI nr
Match: KAG7015844.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 708.8 bits (1828), Expect = 3.6e-200
Identity = 366/528 (69.32%), Postives = 425/528 (80.49%), Query Frame = 0

Query: 6   PANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRISPGDL 65
           PAN LPLSPVTF KRAAA+Y  R S+IYG+R FTW++TY R L LASAL+HHF ISPGD+
Sbjct: 8   PANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHISPGDV 67

Query: 66  VAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFLPILL 125
           VAAMAPN+PELYELH+AVPMAGAIISPLNTKLD+ TL+LLL QL PK+IF+DS FLP +L
Sbjct: 68  VAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVL 127

Query: 126 QSLSQNNANSIKPAAVVIVIPTGPPEVPS----YDGVLAMGSDDFTPCPNAELDPISVNC 185
           +SLSQ   NSI+  A+V+ IP  P   PS    Y+ VL M  DDFTP  NAELD IS+NC
Sbjct: 128 KSLSQTYGNSIQFPALVL-IPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELDAISINC 187

Query: 186 TSGSTGLHKCVVYSHRATYLNSLATILRTQICR-SAPPVFLWTVDMFRCNGWCFVWAVAA 245
           TSGSTGLHK VVYSHRA YLNSLATI R+ IC+ ++ PVFLWTVDMFRCNGWCF+WA+AA
Sbjct: 188 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 247

Query: 246 VGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAP--NRKPPPPLRRVDLIVA 305
           +GGCN+CLR VTA+AIF  +ELH VTL CGP T+LK+I E+   N   P   RRVDLIVA
Sbjct: 248 LGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVA 307

Query: 306 GALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSL-LEDLITSLEVDVK 365
           GALP+ EIL++VT+ GFNISYGYGMTEA+GP +IRP        ++  EDL TSLE+DVK
Sbjct: 308 GALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTSLEIDVK 367

Query: 366 DSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGDWYRTGDVAVRHRSGRIE 425
           D  SMESV  DGETLGE+MLRGNTLMSGY++N KAT+ AFSGDWYRTGDV VRH+SGRIE
Sbjct: 368 DPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRHKSGRIE 427

Query: 426 MKDRANDVIVTADG-AAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIRPCGFVKLRNG 485
           MKDRA D++V A+G A VSTV+VEAVL+SHP VAEAAVVG+        R CGFVKL+NG
Sbjct: 428 MKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGE--------RLCGFVKLKNG 487

Query: 486 CRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNVK 525
            RASAEEIV FC  HLPEFM+P  +VFGDLP+NS GKVQKFI+R+  K
Sbjct: 488 SRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAK 526

BLAST of MS020983 vs. NCBI nr
Match: XP_022923197.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 706.8 bits (1823), Expect = 1.4e-199
Identity = 364/528 (68.94%), Postives = 425/528 (80.49%), Query Frame = 0

Query: 6   PANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRISPGDL 65
           PAN +PLSPVTFLK++AA+Y  R S+IYG+R FTW++TY R L LASAL+HHF +SPGD+
Sbjct: 56  PANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHVSPGDV 115

Query: 66  VAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFLPILL 125
           VAAMAPN+PELYELH+AVPMAGAIISPLNTKLD+ TL+LLL QL PK+IF+DS FLP +L
Sbjct: 116 VAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVL 175

Query: 126 QSLSQNNANSIKPAAVVIVIPTGPPEVPS----YDGVLAMGSDDFTPCPNAELDPISVNC 185
           QSLSQ   NSI+  A+V+ IP  P   PS    Y+ VL M  DDFTP  NAELD IS+NC
Sbjct: 176 QSLSQTYGNSIQFPALVL-IPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELDAISINC 235

Query: 186 TSGSTGLHKCVVYSHRATYLNSLATILRTQICR-SAPPVFLWTVDMFRCNGWCFVWAVAA 245
           TSGSTGLHK VVYSHRA YLNSLATI R+ IC+ ++ PVFLWTVDMFRCNGWCF+WA+AA
Sbjct: 236 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 295

Query: 246 VGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAP--NRKPPPPLRRVDLIVA 305
           +GGCN+CLR VT +AIF  +ELH VTL CGP T+LK+I E+   N   P   RRVDLIVA
Sbjct: 296 LGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRRVDLIVA 355

Query: 306 GALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSL-LEDLITSLEVDVK 365
           GALP+ EIL++VT+ GFNISYGYGMTEA+GP +IRP        ++  EDLITSLE+DVK
Sbjct: 356 GALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITSLEIDVK 415

Query: 366 DSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGDWYRTGDVAVRHRSGRIE 425
           D  SMESV  DGETLGE+MLRGNTLMSGY++N KAT+ AFSGDWYRTGDV VRH+SGRIE
Sbjct: 416 DPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRHKSGRIE 475

Query: 426 MKDRANDVIVTADG-AAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIRPCGFVKLRNG 485
           MKDRA D++V A+G  AVSTV+VEAVL+SHP VAEAAVVG+        R CGFVKL+NG
Sbjct: 476 MKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVVGE--------RLCGFVKLKNG 535

Query: 486 CRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNVK 525
            RASAEEIV FC  HLPEFM+P  +VFGDLP+NS GKVQKFI R+  K
Sbjct: 536 SRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAK 574

BLAST of MS020983 vs. NCBI nr
Match: XP_023552791.1 (probable acyl-activating enzyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 704.9 bits (1818), Expect = 5.2e-199
Identity = 361/528 (68.37%), Postives = 423/528 (80.11%), Query Frame = 0

Query: 6   PANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRISPGDL 65
           PAN +PLSP+TF  ++AA+YG R S+IYG R FTW++TY RCL LASAL+HHF ISPGD+
Sbjct: 8   PANFVPLSPLTFFNKSAAVYGARPSLIYGARVFTWSETYGRCLHLASALVHHFHISPGDV 67

Query: 66  VAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFLPILL 125
           VAAMAPN+PELYELH+AVPMAGAIISPLNTKLD+ TL+LLL QL PK+IF+DS FLP +L
Sbjct: 68  VAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLDPKLIFLDSDFLPTVL 127

Query: 126 QSLSQNNANSIKPAAVVIVIPTGP----PEVPSYDGVLAMGSDDFTPCPNAELDPISVNC 185
           +SLSQ   NS++  A+V+ IP  P     E   Y+ VL M  DDFTP  NAELD IS+NC
Sbjct: 128 KSLSQTYGNSVQFPALVL-IPAHPDTPLSEFLDYNRVLGMRFDDFTPRTNAELDAISINC 187

Query: 186 TSGSTGLHKCVVYSHRATYLNSLATILRTQICR-SAPPVFLWTVDMFRCNGWCFVWAVAA 245
           TSGSTGLHK VVYSHRA YLNSLATI R+ IC+ ++ PVFLWTVDMFRCNGWCF+WA+AA
Sbjct: 188 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 247

Query: 246 VGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAP--NRKPPPPLRRVDLIVA 305
           +GGCN+CLR VT   IF  +ELH VTL CGP T+LK+I E+   N   P   RRVDLIVA
Sbjct: 248 LGGCNICLRNVTTNVIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRRVDLIVA 307

Query: 306 GALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSL-LEDLITSLEVDVK 365
           GALP+ EIL++VT+ GFNISYGYGMTEA+GP +IRP     G  ++  EDLITSLE+DVK
Sbjct: 308 GALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFGEETVQFEDLITSLEIDVK 367

Query: 366 DSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGDWYRTGDVAVRHRSGRIE 425
           D  SMESV  DGETLGE+MLRGNTLMSGY++N KAT+ AFSGDWYRTGDV VRH+SGRIE
Sbjct: 368 DPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRHKSGRIE 427

Query: 426 MKDRANDVIVTADG-AAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIRPCGFVKLRNG 485
           MKDRA D++V A+G AAVSTVEVEAVL+SHP V EAAV+G+        R CGFVKL+NG
Sbjct: 428 MKDRAKDIVVRANGEAAVSTVEVEAVLMSHPSVVEAAVIGE--------RLCGFVKLKNG 487

Query: 486 CRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNVK 525
            RASAEEIV FC  HLPEFM+P  +VFGDLP+NS GKVQKFI+R+  K
Sbjct: 488 SRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAK 526

BLAST of MS020983 vs. NCBI nr
Match: XP_023007650.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 702.6 bits (1812), Expect = 2.6e-198
Identity = 361/528 (68.37%), Postives = 424/528 (80.30%), Query Frame = 0

Query: 6   PANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRISPGDL 65
           PAN +PLSP+ F KRAAA+YGGR S+IYG+R FTW++TY RCL LASAL+HHF ISPGD+
Sbjct: 49  PANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHFHISPGDV 108

Query: 66  VAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFLPILL 125
           VAAMAPN+PELYELH+AVPMAGAIISPLNTKLD+ TL+LLL QL PK+IF+DS FLP LL
Sbjct: 109 VAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDSDFLPTLL 168

Query: 126 QSLSQNNANSIKPAAVVIVIPTGPPEVPS----YDGVLAMGSDDFTPCPNAELDPISVNC 185
           QSLSQ   NSI+  A+V+ IP  P   PS    Y+ VL M  D+FTP  N+ELD IS+NC
Sbjct: 169 QSLSQTYGNSIQFPALVL-IPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELDAISINC 228

Query: 186 TSGSTGLHKCVVYSHRATYLNSLATILRTQICR-SAPPVFLWTVDMFRCNGWCFVWAVAA 245
           TSGSTGLHK VVYSHRA YLNSLATI R+ IC+ ++ PVFLWTVDMFRCNGWCF+WA+AA
Sbjct: 229 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 288

Query: 246 VGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAP--NRKPPPPLRRVDLIVA 305
           +GGCN+CLR VT +AIF  +ELH VTL CGP T+LK+I E+   N   P  LRRVDLIVA
Sbjct: 289 LGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRRVDLIVA 348

Query: 306 GALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSL-LEDLITSLEVDVK 365
           GALP+ EIL++VT+ GFNISYGYGMTEA+GP +IRP        ++  EDLI SLE+DVK
Sbjct: 349 GALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIASLEIDVK 408

Query: 366 DSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGDWYRTGDVAVRHRSGRIE 425
           D +SMESV  DGETLGE+MLRGN+LMSGY++N KAT  AFSGDWYRTGDV V+H+SGRIE
Sbjct: 409 DPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKHKSGRIE 468

Query: 426 MKDRANDVIVTADG-AAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIRPCGFVKLRNG 485
           MKDRA D++V  +G  AVSTV+VEAVL+SHP VAEAAV+G+        R CG VKL+NG
Sbjct: 469 MKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVIGE--------RLCGLVKLKNG 528

Query: 486 CRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNVK 525
            RASAEEIV FC  HLPEFM+P  +VFGDLP+NS GKVQKFI R+  K
Sbjct: 529 SRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAK 567

BLAST of MS020983 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.9e-127
Identity = 249/541 (46.03%), Postives = 344/541 (63.59%), Query Frame = 0

Query: 1   GLLHCPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRI 60
           GL+ C AN +PLSP+TFL+R++  Y   TS++YG+  +TWA T++RCLKLASAL  HF I
Sbjct: 31  GLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHFGI 90

Query: 61  SPGDLVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQF 120
           SPGD+VA  + NIPE+YELH+AVPMAG I+  LN + DSA ++ LL     K+IFV+ Q 
Sbjct: 91  SPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEPQL 150

Query: 121 LPILLQSLSQNNANSIKPAAVVIVIPTGPPEVPSYD---GVLAMGSDDF-TPCPNAELDP 180
           L     +L       IKP  +V++  +      SYD    +LA GSDDF    P  E DP
Sbjct: 151 LETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNECDP 210

Query: 181 ISVNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRSAPPVFLWTVDMFRCNGWCFVW 240
           IS+N TSG+T   K VVYSHR  YLNS+AT+L   +      V+LW+V MF CNGWCF W
Sbjct: 211 ISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGM--GTRSVYLWSVPMFHCNGWCFPW 270

Query: 241 AVAAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAPNRKPPPPL-RRVDL 300
             AA G  N+C+R V+ +AIF+ + LH VT F   PT+L +I  +P      PL  +V++
Sbjct: 271 GAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEV 330

Query: 301 IVAGALPVGEILSRVTQYGFNISYGYGMTEALGPV----------IIRPISGGGGGVSLL 360
           +  G+ P  ++++R+ + GF +++ YG+TE  GP            ++P           
Sbjct: 331 MTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYALKARQG 390

Query: 361 EDLITSLEVDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGDWYRTG 420
            + +   E+DV+D  SMESV ADG T+GE+M RGNT+MSGYF++ KAT+ AF G W+RTG
Sbjct: 391 LNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFRTG 450

Query: 421 DVAVRHRSGRIEMKDRANDVIVTADGAAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEI 480
           D+ V+H  G I++KDR  DV+++  G  VSTVEVE VL SH  V EAAVV + +  +GE 
Sbjct: 451 DLGVKHEDGYIQLKDRKKDVVISG-GENVSTVEVETVLYSHEAVLEAAVVARPDKLWGE- 510

Query: 481 RPCGFVKLRNGC--RASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNV 525
            PC FV L+ G     SA++I+ FC D LP +M P ++VF +LP  STGK+QK+I+++  
Sbjct: 511 TPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEKA 567

BLAST of MS020983 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.8e-125
Identity = 255/552 (46.20%), Postives = 348/552 (63.04%), Query Frame = 0

Query: 1   GLLHCPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRI 60
           GLL  PAN  PLSP+TFL+R+A +Y  RTS+++G+   TW  TY RCL+LASAL  +  I
Sbjct: 50  GLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NLGI 109

Query: 61  SPGDLVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQF 120
           S GD+VAA+APN+P ++ELH+AVPMAG I+ PLNT+LD +TL++LL     KI+FVD Q 
Sbjct: 110 SRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQL 169

Query: 121 LPILLQSLS--QNNANSIKPAAVVIVIPTGPPEVP----------------SYDGVLAMG 180
           L I   +L     +  + K   +V++  +   +                   Y+ +L  G
Sbjct: 170 LEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSG 229

Query: 181 SDDFTPC-PNAELDPISVNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRSAPPVFL 240
             +F    P  E DPIS+N TSG+T   K VVYSHR  YLNSLAT+   Q+  S  PV+L
Sbjct: 230 DSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYPVYL 289

Query: 241 WTVDMFRCNGWCFVWAVAAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEA 300
           WTV MF CNGWC VW VAA GG N+CLR V+ + IF  + +H VT   G PT+L +I   
Sbjct: 290 WTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNY 349

Query: 301 PNRKPPPPLRRVDLIVAGALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGG 360
              +  P   RV+++  G+ P+ +IL+++ + GFN+S+ YG+TE  GP            
Sbjct: 350 TVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDS 409

Query: 361 VSLLEDLITSLE------------VDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFEN 420
           +SL E   T L+            +DVKD  +ME+V  DG T+GE+M RGNT+MSGYF++
Sbjct: 410 LSLEER--TKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKD 469

Query: 421 PKATKAAFSGDWYRTGDVAVRHRSGRIEMKDRANDVIVTADGAAVSTVEVEAVLLSHPEV 480
            +AT+ AF GDW+ +GD+AV++  G IE+KDR  DVI++  G  +S+VEVE VL SH  V
Sbjct: 470 IEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISG-GENISSVEVERVLCSHQAV 529

Query: 481 AEAAVVGKYEAGFGEIRPCGFVKLRNGC-RASAEEIVGFCGDHLPEFMVPASIVFGDLPL 521
            EAAVV + +  +G+  PCGFVKL+ G      EEI+GFC DHLP +M P +IVFGD+P 
Sbjct: 530 LEAAVVARPDHHWGQ-TPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 589

BLAST of MS020983 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 3.0e-125
Identity = 243/538 (45.17%), Postives = 342/538 (63.57%), Query Frame = 0

Query: 1   GLLHCPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRI 60
           GL+ C AN +PLSP+TFL+R++  Y   TS++YG+  +TWA T++RCLKLASAL  H  I
Sbjct: 31  GLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHLGI 90

Query: 61  SPGDLVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQF 120
           SPGD+VA  + N+PE+YELH+AVPMAG I+  LN + DSA ++ LL     K+IFV+ Q 
Sbjct: 91  SPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEPQL 150

Query: 121 LPILLQSLSQNNANSIKPAAVVIVIPTGPPEVPSYD---GVLAMGSDDF-TPCPNAELDP 180
           L     +L       IKP  +V++  +      SYD    +LA GSDDF    P  E DP
Sbjct: 151 LETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEWDP 210

Query: 181 ISVNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRSAPPVFLWTVDMFRCNGWCFVW 240
           IS+N TSG+T   K VVYSHR  YLNS+AT+L   +      V+LW+V MF CNGWCF W
Sbjct: 211 ISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGM--GTTSVYLWSVPMFHCNGWCFPW 270

Query: 241 AVAAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAPNRKPPPPL-RRVDL 300
             AA G  N+C+R V+ +AIF+ + LH VT F   PT+L +I  +P      PL  +V++
Sbjct: 271 GAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEV 330

Query: 301 IVAGALPVGEILSRVTQYGFNISYGYGMTEALGPV----------IIRPISGGGGGVSLL 360
           +  G+ P  ++++R+ + GF +++ YG+TE  GP            ++P           
Sbjct: 331 MTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKARQG 390

Query: 361 EDLITSLEVDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGDWYRTG 420
            + +   E+DV+D  +MESV ADG T+GE+M RGNT+MSGYF++ KAT+ AF G W+R+G
Sbjct: 391 LNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFRSG 450

Query: 421 DVAVRHRSGRIEMKDRANDVIVTADGAAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEI 480
           D+ V+H  G I++KDR  DV+++  G  +STVEVE VL SH  V EAAVV + +  +GE 
Sbjct: 451 DLGVKHEDGYIQLKDRKKDVVISG-GENISTVEVETVLYSHEAVLEAAVVARPDKLWGE- 510

Query: 481 RPCGFVKLRNGC--RASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRD 522
            PC FV L+ G     SA++I+ FC D LP +M P ++VF +LP  STGK+QK+I+++
Sbjct: 511 TPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKE 564

BLAST of MS020983 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 7.0e-122
Identity = 237/545 (43.49%), Postives = 335/545 (61.47%), Query Frame = 0

Query: 1   GLLHCPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRI 60
           G +  PAN +PL+P++FL R+A +Y  R SI+YG+  +TW  T  RC+++ASAL     I
Sbjct: 5   GTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQLGI 64

Query: 61  SPGDLVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQF 120
           S GD+V+ +APN+P + ELH+ VPMAGA++  LN + DS+ +A+LL     K+IF D QF
Sbjct: 65  STGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQF 124

Query: 121 LPILLQSLSQNNANSIKPAAVVIVIP----------TGPPEVPSYDGVLAMGSDDFTPC- 180
           L I  +   +  +N      ++++IP              E+  Y+ V+AMG  DF    
Sbjct: 125 LQI-AEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 184

Query: 181 PNAELDPISVNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRSAPPVFLWTVDMFRC 240
           P  E D ISVN TSG+T   K VVYSHR  YLNSLA +L  ++  S  P +LWT  MF C
Sbjct: 185 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSS--PTYLWTNPMFHC 244

Query: 241 NGWCFVWAVAAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAPNRKPPPP 300
           NGWC +W V A+GG N+CLR VTA+AIF+ +  H VT   G PTIL +I  AP  +  P 
Sbjct: 245 NGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPL 304

Query: 301 LRRVDLIVAGALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSLLEDL- 360
             +V  I   A P   ++ ++ + GF++ + YG+TE  GP  I         +   E   
Sbjct: 305 PGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAK 364

Query: 361 ---------ITSLEVDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSG 420
                    +   E+ VKD  +M ++ ADG T+GE++ RGNT+M+GY +NP+ATK AF G
Sbjct: 365 MKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKG 424

Query: 421 DWYRTGDVAVRHRSGRIEMKDRANDVIVTADGAAVSTVEVEAVLLSHPEVAEAAVVGKYE 480
            W+ +GD+ V+H  G IE+KDR+ D+I++  G  +S++EVE+ L +HP V EAAVV + +
Sbjct: 425 GWFWSGDLGVKHPDGYIELKDRSKDIIISG-GENISSIEVESTLFTHPCVLEAAVVARPD 484

Query: 481 AGFGEIRPCGFVKLRNGCRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFII 525
             +GE   C FVKL++G +ASAEE++ +C D LP +M P SIVF DLP  STGKVQKF++
Sbjct: 485 EYWGE-TACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVL 543

BLAST of MS020983 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 4.7e-110
Identity = 223/550 (40.55%), Postives = 334/550 (60.73%), Query Frame = 0

Query: 2   LLHCPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRIS 61
           L+ C AN +PL+P+TFLKRA+  Y  RTSIIYG   FTW  TY RC +LA++L+    I+
Sbjct: 4   LVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SLNIT 63

Query: 62  PGDLVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFL 121
             D+V+ +APN+P +YE+H++VPM GA+++P+NT+LD+ T+A++L    PKI+FVD +F 
Sbjct: 64  RNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYEFA 123

Query: 122 PILLQSLSQNNANSIKPAAVVIVI-----PTGP-PEVPSYDGVLAMGSDDFTPCPNA--- 181
           P++ + L        +P   +I+I      T P  +   Y+G++  G    TP  +A   
Sbjct: 124 PLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEP--TPSSSASMF 183

Query: 182 ----ELDPISVNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRSAPPVFLWTVDMFR 241
               E DPIS+N TSG+T   K VV SH+  YL++L++I+  ++     PV+LWT+ MF 
Sbjct: 184 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEM--GIFPVYLWTLPMFH 243

Query: 242 CNGWCFVWAVAAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAPNRKPPP 301
           CNGW   W+VAA GG NVC+R VTA  I+  +ELHGVT     PT+ + + E       P
Sbjct: 244 CNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSP 303

Query: 302 PLRRVDLIVAGALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSLLEDL 361
               V ++  G+ P   ++ +V Q GF++ +GYG+TEA GPV+          +   + +
Sbjct: 304 KSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQI 363

Query: 362 ----------ITSLEVDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFS 421
                     +T  +VDVK++ ++ESV  DG+T+GEI+++G++LM GY +NPKAT  AF 
Sbjct: 364 ELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFK 423

Query: 422 GDWYRTGDVAVRHRSGRIEMKDRANDVIVTADGAAVSTVEVEAVLLSHPEVAEAAVVGKY 481
             W  TGD+ V H  G +E+KDR+ D+I++  G  +S++EVE VL  + EV EAAVV   
Sbjct: 424 HGWLNTGDIGVIHPDGYVEIKDRSKDIIISG-GENISSIEVEKVLYMYQEVLEAAVVAMP 483

Query: 482 EAGFGEIRPCGFVKLR---NGCRASAEEIVGFCGDHLPEFMVPASIV-FGDLPLNSTGKV 525
              +GE  PC FV L+    G   S  +++ +C +++P FM P  +V F +LP NS GK+
Sbjct: 484 HPLWGE-TPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKI 543

BLAST of MS020983 vs. ExPASy TrEMBL
Match: A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 6.6e-200
Identity = 364/528 (68.94%), Postives = 425/528 (80.49%), Query Frame = 0

Query: 6   PANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRISPGDL 65
           PAN +PLSPVTFLK++AA+Y  R S+IYG+R FTW++TY R L LASAL+HHF +SPGD+
Sbjct: 56  PANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHVSPGDV 115

Query: 66  VAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFLPILL 125
           VAAMAPN+PELYELH+AVPMAGAIISPLNTKLD+ TL+LLL QL PK+IF+DS FLP +L
Sbjct: 116 VAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVL 175

Query: 126 QSLSQNNANSIKPAAVVIVIPTGPPEVPS----YDGVLAMGSDDFTPCPNAELDPISVNC 185
           QSLSQ   NSI+  A+V+ IP  P   PS    Y+ VL M  DDFTP  NAELD IS+NC
Sbjct: 176 QSLSQTYGNSIQFPALVL-IPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELDAISINC 235

Query: 186 TSGSTGLHKCVVYSHRATYLNSLATILRTQICR-SAPPVFLWTVDMFRCNGWCFVWAVAA 245
           TSGSTGLHK VVYSHRA YLNSLATI R+ IC+ ++ PVFLWTVDMFRCNGWCF+WA+AA
Sbjct: 236 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 295

Query: 246 VGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAP--NRKPPPPLRRVDLIVA 305
           +GGCN+CLR VT +AIF  +ELH VTL CGP T+LK+I E+   N   P   RRVDLIVA
Sbjct: 296 LGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRRVDLIVA 355

Query: 306 GALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSL-LEDLITSLEVDVK 365
           GALP+ EIL++VT+ GFNISYGYGMTEA+GP +IRP        ++  EDLITSLE+DVK
Sbjct: 356 GALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITSLEIDVK 415

Query: 366 DSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGDWYRTGDVAVRHRSGRIE 425
           D  SMESV  DGETLGE+MLRGNTLMSGY++N KAT+ AFSGDWYRTGDV VRH+SGRIE
Sbjct: 416 DPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRHKSGRIE 475

Query: 426 MKDRANDVIVTADG-AAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIRPCGFVKLRNG 485
           MKDRA D++V A+G  AVSTV+VEAVL+SHP VAEAAVVG+        R CGFVKL+NG
Sbjct: 476 MKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVVGE--------RLCGFVKLKNG 535

Query: 486 CRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNVK 525
            RASAEEIV FC  HLPEFM+P  +VFGDLP+NS GKVQKFI R+  K
Sbjct: 536 SRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAK 574

BLAST of MS020983 vs. ExPASy TrEMBL
Match: A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 1.2e-198
Identity = 361/528 (68.37%), Postives = 424/528 (80.30%), Query Frame = 0

Query: 6   PANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRISPGDL 65
           PAN +PLSP+ F KRAAA+YGGR S+IYG+R FTW++TY RCL LASAL+HHF ISPGD+
Sbjct: 49  PANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHFHISPGDV 108

Query: 66  VAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFLPILL 125
           VAAMAPN+PELYELH+AVPMAGAIISPLNTKLD+ TL+LLL QL PK+IF+DS FLP LL
Sbjct: 109 VAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDSDFLPTLL 168

Query: 126 QSLSQNNANSIKPAAVVIVIPTGPPEVPS----YDGVLAMGSDDFTPCPNAELDPISVNC 185
           QSLSQ   NSI+  A+V+ IP  P   PS    Y+ VL M  D+FTP  N+ELD IS+NC
Sbjct: 169 QSLSQTYGNSIQFPALVL-IPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELDAISINC 228

Query: 186 TSGSTGLHKCVVYSHRATYLNSLATILRTQICR-SAPPVFLWTVDMFRCNGWCFVWAVAA 245
           TSGSTGLHK VVYSHRA YLNSLATI R+ IC+ ++ PVFLWTVDMFRCNGWCF+WA+AA
Sbjct: 229 TSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAA 288

Query: 246 VGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAP--NRKPPPPLRRVDLIVA 305
           +GGCN+CLR VT +AIF  +ELH VTL CGP T+LK+I E+   N   P  LRRVDLIVA
Sbjct: 289 LGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRRVDLIVA 348

Query: 306 GALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSL-LEDLITSLEVDVK 365
           GALP+ EIL++VT+ GFNISYGYGMTEA+GP +IRP        ++  EDLI SLE+DVK
Sbjct: 349 GALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIASLEIDVK 408

Query: 366 DSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGDWYRTGDVAVRHRSGRIE 425
           D +SMESV  DGETLGE+MLRGN+LMSGY++N KAT  AFSGDWYRTGDV V+H+SGRIE
Sbjct: 409 DPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKHKSGRIE 468

Query: 426 MKDRANDVIVTADG-AAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIRPCGFVKLRNG 485
           MKDRA D++V  +G  AVSTV+VEAVL+SHP VAEAAV+G+        R CG VKL+NG
Sbjct: 469 MKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVIGE--------RLCGLVKLKNG 528

Query: 486 CRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNVK 525
            RASAEEIV FC  HLPEFM+P  +VFGDLP+NS GKVQKFI R+  K
Sbjct: 529 SRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAK 567

BLAST of MS020983 vs. ExPASy TrEMBL
Match: A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.8e-189
Identity = 355/538 (65.99%), Postives = 413/538 (76.77%), Query Frame = 0

Query: 1   GLLHCPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRI 60
           G  HCP N  PLSPV FLKRA+ LYG R S++YG+R FTW+ TY RCL +ASAL+HHF +
Sbjct: 3   GFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHFHL 62

Query: 61  SPGDLVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQF 120
           SP DLV AMAPNIPELYELH+AVPMAG IIS LNTKLDS TL+LLL QL PK+IF+DSQF
Sbjct: 63  SPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQF 122

Query: 121 LPILLQSLSQNNANSIKPAAVVIVIPTGPPEVPS----YDGVLAM--GSDDFTPCPNAEL 180
           LPILL+SL      SIK  A++++       +PS    Y+ +L M  G D+FTP PNAEL
Sbjct: 123 LPILLKSLEN---ISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAEL 182

Query: 181 DPISVNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRS-APPVFLWTVDMFRCNGWC 240
           DPIS+N TSGSTGLHK V+YSHRA YLNSLATI R++IC S + PVFLWTVDMFRCNGWC
Sbjct: 183 DPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWC 242

Query: 241 FVWAVAAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLI--SEAPNRKPPPPLR 300
           F+W +AA+GGCN+CLRTVTA+AIF  VELH VTL CGP T+LK+I  S + N  P    R
Sbjct: 243 FIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSR 302

Query: 301 RVDLIVAGALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGG---GGGVSLLEDL 360
           RVDLIVAGALP+ EIL++V + GFNISYGYGMTEA+GP IIRP          V   + L
Sbjct: 303 RVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHL 362

Query: 361 ITSLEVDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGD-WYRTGDV 420
           ITSLE+DVKD  SMESV  DGETLGE+MLRGNTLMSGY++N KAT  AF G+ WYRTGDV
Sbjct: 363 ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDV 422

Query: 421 AVRHRSGRIEMKDRANDVIVTADG-AAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIR 480
            VRH+SGRIEMKDRA D++V  DG  AVSTVEVE VL+SHP VAE AVV       GE  
Sbjct: 423 GVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVV-------GERT 482

Query: 481 PCGFVKLRNGCRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNVK 525
             GFVKL+NG + + +EIV FC  HLPEFMVP +IVFGDLP+NSTGKVQKF++R+  K
Sbjct: 483 LYGFVKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAK 530

BLAST of MS020983 vs. ExPASy TrEMBL
Match: A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 3.4e-164
Identity = 320/469 (68.23%), Postives = 367/469 (78.25%), Query Frame = 0

Query: 69  MAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFLPILLQSL 128
           MAPNIPELYELH+AVPMAGAIIS LNTKLDS TL+LLL QL PK+IF+DSQFLPILL+SL
Sbjct: 1   MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60

Query: 129 SQNNANSIKPAAVVIVIPTG-----PPEVPSYDGVLAM--GSDDFTPCPNAELDPISVNC 188
                +SIK  A+V+ IP+      P E   Y+ VLAM  G DDFTP PNAELDPIS+N 
Sbjct: 61  EN---SSIKFPALVL-IPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINY 120

Query: 189 TSGSTGLHKCVVYSHRATYLNSLATILRTQICRS-APPVFLWTVDMFRCNGWCFVWAVAA 248
           TSGSTGLHK V+YSHRA YLNSLATI R++IC S + PVFLWTVDMFRCNGWCF+W +AA
Sbjct: 121 TSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAA 180

Query: 249 VGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLI--SEAPNRKPPPPLRRVDLIVA 308
           +GGCN+CLRTVTA+AIF  VELH VTL CGPPT+LK+I  S + N  P    RRVDLIVA
Sbjct: 181 LGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVA 240

Query: 309 GALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSL-LEDLITSLEVDVK 368
           GALP+ EIL++V + GFNISYGYGMTEA+GP IIRP        ++  EDLITSLE+DVK
Sbjct: 241 GALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVK 300

Query: 369 DSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGD-WYRTGDVAVRHRSGRI 428
           D  SMESV  DGETLGE+MLRGNTLMSGY++N KAT  AF G+ WYRTGDV VRH+SGRI
Sbjct: 301 DPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRI 360

Query: 429 EMKDRANDVIVTADG-AAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIRPCGFVKLRN 488
           EMKDRA D++V  DG  AVSTVEVE VL+SHP VAEAAVV       GE    GFVKL+N
Sbjct: 361 EMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVV-------GERTLYGFVKLKN 420

Query: 489 GCRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNVK 525
             + + +EIV FC  HLPEFM+P  IVFGDLP+NSTGKVQKF +R+ VK
Sbjct: 421 RSKENGDEIVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVK 458

BLAST of MS020983 vs. ExPASy TrEMBL
Match: A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.8e-160
Identity = 311/470 (66.17%), Postives = 361/470 (76.81%), Query Frame = 0

Query: 69  MAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFLPILLQSL 128
           MAPNIPELYELH+AVPMAG IIS LNTKLDS TL+LLL QL PK+IF+DSQFLPILL+SL
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 129 SQNNANSIKPAAVVIVIPTGPPEVPS----YDGVLAM--GSDDFTPCPNAELDPISVNCT 188
                 SIK  A++++       +PS    Y+ +L M  G D+FTP PNAELDPIS+N T
Sbjct: 61  EN---ISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYT 120

Query: 189 SGSTGLHKCVVYSHRATYLNSLATILRTQICRS-APPVFLWTVDMFRCNGWCFVWAVAAV 248
           SGSTGLHK V+YSHRA YLNSLATI R++IC S + PVFLWTVDMFRCNGWCF+W +AA+
Sbjct: 121 SGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAAL 180

Query: 249 GGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLI--SEAPNRKPPPPLRRVDLIVAG 308
           GGCN+CLRTVTA+AIF  VELH VTL CGP T+LK+I  S + N  P    RRVDLIVAG
Sbjct: 181 GGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAG 240

Query: 309 ALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGG---GGGVSLLEDLITSLEVDV 368
           ALP+ EIL++V + GFNISYGYGMTEA+GP IIRP          V   + LITSLE+DV
Sbjct: 241 ALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDV 300

Query: 369 KDSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGD-WYRTGDVAVRHRSGR 428
           KD  SMESV  DGETLGE+MLRGNTLMSGY++N KAT  AF G+ WYRTGDV VRH+SGR
Sbjct: 301 KDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGR 360

Query: 429 IEMKDRANDVIVTADG-AAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIRPCGFVKLR 488
           IEMKDRA D++V  DG  AVSTVEVE VL+SHP VAE AVV       GE    GFVKL+
Sbjct: 361 IEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVV-------GERTLYGFVKLK 420

Query: 489 NGCRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFIIRDNVK 525
           NG + + +EIV FC  HLPEFMVP +IVFGDLP+NSTGKVQKF++R+  K
Sbjct: 421 NGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAK 460

BLAST of MS020983 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 451.1 bits (1159), Expect = 1.3e-126
Identity = 255/552 (46.20%), Postives = 348/552 (63.04%), Query Frame = 0

Query: 1   GLLHCPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRI 60
           GLL  PAN  PLSP+TFL+R+A +Y  RTS+++G+   TW  TY RCL+LASAL  +  I
Sbjct: 50  GLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NLGI 109

Query: 61  SPGDLVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQF 120
           S GD+VAA+APN+P ++ELH+AVPMAG I+ PLNT+LD +TL++LL     KI+FVD Q 
Sbjct: 110 SRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQL 169

Query: 121 LPILLQSLS--QNNANSIKPAAVVIVIPTGPPEVP----------------SYDGVLAMG 180
           L I   +L     +  + K   +V++  +   +                   Y+ +L  G
Sbjct: 170 LEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSG 229

Query: 181 SDDFTPC-PNAELDPISVNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRSAPPVFL 240
             +F    P  E DPIS+N TSG+T   K VVYSHR  YLNSLAT+   Q+  S  PV+L
Sbjct: 230 DSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYPVYL 289

Query: 241 WTVDMFRCNGWCFVWAVAAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEA 300
           WTV MF CNGWC VW VAA GG N+CLR V+ + IF  + +H VT   G PT+L +I   
Sbjct: 290 WTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNY 349

Query: 301 PNRKPPPPLRRVDLIVAGALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGG 360
              +  P   RV+++  G+ P+ +IL+++ + GFN+S+ YG+TE  GP            
Sbjct: 350 TVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDS 409

Query: 361 VSLLEDLITSLE------------VDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFEN 420
           +SL E   T L+            +DVKD  +ME+V  DG T+GE+M RGNT+MSGYF++
Sbjct: 410 LSLEER--TKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKD 469

Query: 421 PKATKAAFSGDWYRTGDVAVRHRSGRIEMKDRANDVIVTADGAAVSTVEVEAVLLSHPEV 480
            +AT+ AF GDW+ +GD+AV++  G IE+KDR  DVI++  G  +S+VEVE VL SH  V
Sbjct: 470 IEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISG-GENISSVEVERVLCSHQAV 529

Query: 481 AEAAVVGKYEAGFGEIRPCGFVKLRNGC-RASAEEIVGFCGDHLPEFMVPASIVFGDLPL 521
            EAAVV + +  +G+  PCGFVKL+ G      EEI+GFC DHLP +M P +IVFGD+P 
Sbjct: 530 LEAAVVARPDHHWGQ-TPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 589

BLAST of MS020983 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 439.1 bits (1128), Expect = 4.9e-123
Identity = 237/545 (43.49%), Postives = 335/545 (61.47%), Query Frame = 0

Query: 1   GLLHCPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRI 60
           G +  PAN +PL+P++FL R+A +Y  R SI+YG+  +TW  T  RC+++ASAL     I
Sbjct: 5   GTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQLGI 64

Query: 61  SPGDLVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQF 120
           S GD+V+ +APN+P + ELH+ VPMAGA++  LN + DS+ +A+LL     K+IF D QF
Sbjct: 65  STGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQF 124

Query: 121 LPILLQSLSQNNANSIKPAAVVIVIP----------TGPPEVPSYDGVLAMGSDDFTPC- 180
           L I  +   +  +N      ++++IP              E+  Y+ V+AMG  DF    
Sbjct: 125 LQI-AEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 184

Query: 181 PNAELDPISVNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRSAPPVFLWTVDMFRC 240
           P  E D ISVN TSG+T   K VVYSHR  YLNSLA +L  ++  S  P +LWT  MF C
Sbjct: 185 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSS--PTYLWTNPMFHC 244

Query: 241 NGWCFVWAVAAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAPNRKPPPP 300
           NGWC +W V A+GG N+CLR VTA+AIF+ +  H VT   G PTIL +I  AP  +  P 
Sbjct: 245 NGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPL 304

Query: 301 LRRVDLIVAGALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSLLEDL- 360
             +V  I   A P   ++ ++ + GF++ + YG+TE  GP  I         +   E   
Sbjct: 305 PGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAK 364

Query: 361 ---------ITSLEVDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSG 420
                    +   E+ VKD  +M ++ ADG T+GE++ RGNT+M+GY +NP+ATK AF G
Sbjct: 365 MKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKG 424

Query: 421 DWYRTGDVAVRHRSGRIEMKDRANDVIVTADGAAVSTVEVEAVLLSHPEVAEAAVVGKYE 480
            W+ +GD+ V+H  G IE+KDR+ D+I++  G  +S++EVE+ L +HP V EAAVV + +
Sbjct: 425 GWFWSGDLGVKHPDGYIELKDRSKDIIISG-GENISSIEVESTLFTHPCVLEAAVVARPD 484

Query: 481 AGFGEIRPCGFVKLRNGCRASAEEIVGFCGDHLPEFMVPASIVFGDLPLNSTGKVQKFII 525
             +GE   C FVKL++G +ASAEE++ +C D LP +M P SIVF DLP  STGKVQKF++
Sbjct: 485 EYWGE-TACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVL 543

BLAST of MS020983 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 399.8 bits (1026), Expect = 3.3e-111
Identity = 223/550 (40.55%), Postives = 334/550 (60.73%), Query Frame = 0

Query: 2   LLHCPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRIS 61
           L+ C AN +PL+P+TFLKRA+  Y  RTSIIYG   FTW  TY RC +LA++L+    I+
Sbjct: 4   LVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SLNIT 63

Query: 62  PGDLVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFL 121
             D+V+ +APN+P +YE+H++VPM GA+++P+NT+LD+ T+A++L    PKI+FVD +F 
Sbjct: 64  RNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYEFA 123

Query: 122 PILLQSLSQNNANSIKPAAVVIVI-----PTGP-PEVPSYDGVLAMGSDDFTPCPNA--- 181
           P++ + L        +P   +I+I      T P  +   Y+G++  G    TP  +A   
Sbjct: 124 PLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEP--TPSSSASMF 183

Query: 182 ----ELDPISVNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRSAPPVFLWTVDMFR 241
               E DPIS+N TSG+T   K VV SH+  YL++L++I+  ++     PV+LWT+ MF 
Sbjct: 184 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEM--GIFPVYLWTLPMFH 243

Query: 242 CNGWCFVWAVAAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAPNRKPPP 301
           CNGW   W+VAA GG NVC+R VTA  I+  +ELHGVT     PT+ + + E       P
Sbjct: 244 CNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSP 303

Query: 302 PLRRVDLIVAGALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSLLEDL 361
               V ++  G+ P   ++ +V Q GF++ +GYG+TEA GPV+          +   + +
Sbjct: 304 KSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQI 363

Query: 362 ----------ITSLEVDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFS 421
                     +T  +VDVK++ ++ESV  DG+T+GEI+++G++LM GY +NPKAT  AF 
Sbjct: 364 ELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFK 423

Query: 422 GDWYRTGDVAVRHRSGRIEMKDRANDVIVTADGAAVSTVEVEAVLLSHPEVAEAAVVGKY 481
             W  TGD+ V H  G +E+KDR+ D+I++  G  +S++EVE VL  + EV EAAVV   
Sbjct: 424 HGWLNTGDIGVIHPDGYVEIKDRSKDIIISG-GENISSIEVEKVLYMYQEVLEAAVVAMP 483

Query: 482 EAGFGEIRPCGFVKLR---NGCRASAEEIVGFCGDHLPEFMVPASIV-FGDLPLNSTGKV 525
              +GE  PC FV L+    G   S  +++ +C +++P FM P  +V F +LP NS GK+
Sbjct: 484 HPLWGE-TPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKI 543

BLAST of MS020983 vs. TAIR 10
Match: AT1G76290.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 379.0 bits (972), Expect = 6.1e-105
Identity = 223/541 (41.22%), Postives = 319/541 (58.96%), Query Frame = 0

Query: 1   GLLHCPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRI 60
           G + C AN +PLSP++FL+RAA ++G RTS++YG   +TW  T  RC++LASAL     +
Sbjct: 3   GTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASAL-SDLGL 62

Query: 61  SPGDLVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQF 120
           S  D+VAA+APN+P L EL++  PMAGA++  LNT  DS  LA+ L + +PK+ FVDS+F
Sbjct: 63  SRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSEF 122

Query: 121 LPILLQSLSQNNANSIKPAAVVIV-IPTGPPEVPSYDGVLAMGSDDFTPC-PNAELDPIS 180
           L +  +SLS  +    KP  + I   PT   +   Y+  L+ G+ +F P  P  E DPI+
Sbjct: 123 LSVAEESLSLLSNIEEKPLIITITENPTEQSKYEQYEDFLSTGNPNFKPIRPVDECDPIA 182

Query: 181 VNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRSAPPVFLWTVDMFRCNGWCFVWAV 240
           +N TSG+T   KCVVYSHR  YLN+ A  +  ++     PV+L TV M+ C+GWC++W V
Sbjct: 183 LNFTSGTTSTPKCVVYSHRGAYLNATAVGVMNEM--KPMPVYLCTVPMYHCSGWCYIWTV 242

Query: 241 AAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAPNRKPPPPLRRVDLIVA 300
            A GG  VCLR V  E IF+++  H VT F G P +L +I+ A +         V ++  
Sbjct: 243 TAFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKSFPWTVQVMSG 302

Query: 301 GALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSLLEDLITSLE----- 360
           G+ P  E++ ++ + GF +   YG +E  G  +           +L E+    L+     
Sbjct: 303 GSSP-PEVMLKLKKLGFKVMMAYGCSEVYG--LGTACLWMPEWETLPEEESLRLKARDGL 362

Query: 361 -------VDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGDWYRTGD 420
                  VDV D T+M+SV  DG+T+  I LRGNT+MSGYF++ +AT+AAF G WY + D
Sbjct: 363 NHFAKEAVDVLDPTTMKSVPHDGKTIRVIALRGNTVMSGYFKDKEATEAAFRGGWYWSRD 422

Query: 421 VAVRHRSGRIEMKDRANDVIVTADGAAVSTVEVEAVLLSHPEVAEAAVVGKYEAGFGEIR 480
           + V    G I+ KDR+ DVI T  G  V + E+E +L SHP V +A VVG+ +   GE  
Sbjct: 423 MGVIDPDGYIQFKDRSQDVI-TCGGEIVGSKEIEGILYSHPAVYDAGVVGRPDETLGE-S 482

Query: 481 PCGFVKLRNGCRASAEEIVGFCGDHLPE---FMVPASIVFGDLPLNSTGKVQKFIIRDNV 525
            C FVKL+ G  A  EEI+ FC   L      M+P ++VF D+P   TGK++K ++R   
Sbjct: 483 MCAFVKLKEGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKNVLRKMA 535

BLAST of MS020983 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 374.8 bits (961), Expect = 1.1e-103
Identity = 216/555 (38.92%), Postives = 319/555 (57.48%), Query Frame = 0

Query: 5   CPANLLPLSPVTFLKRAAALYGGRTSIIYGTRAFTWADTYYRCLKLASALIHHFRISPGD 64
           C AN +PL+P+TFLKRA+  Y  RTSIIYG   FTW  TY RC +LA++LI    I   D
Sbjct: 7   CEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SLNIGKND 66

Query: 65  LVAAMAPNIPELYELHYAVPMAGAIISPLNTKLDSATLALLLHQLRPKIIFVDSQFLPI- 124
           +V+ +APN P +YE+H+AVPMAGA+++P+NT+LD+ ++A +L   +PKI+F+   F P+ 
Sbjct: 67  VVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRSFEPLA 126

Query: 125 --LLQSLSQNNANSIKPAAVVIVIPTGPPEVPS----YDGVLAMGSDDFTP-------CP 184
             +LQ LS  ++N   P   +  I   P  V S    Y+ ++  G    TP       C 
Sbjct: 127 REILQLLSSEDSNLNLPVIFIHEIDF-PKRVSSEESDYECLIQRGEP--TPLLLARMFCI 186

Query: 185 NAELDPISVNCTSGSTGLHKCVVYSHRATYLNSLATILRTQICRSAPPVFLWTVDMFRCN 244
             E DPIS+N TSG+T   K VV SHR  YL++L+ I+  ++     PV+LWT+ MF CN
Sbjct: 187 QDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEM--GTCPVYLWTLPMFHCN 246

Query: 245 GWCFVWAVAAVGGCNVCLRTVTAEAIFNAVELHGVTLFCGPPTILKLISEAPNRKPPPPL 304
           GW F W  AA GG +VC+R VTA  I+  +E+H VT  C  PT+  ++ +  +       
Sbjct: 247 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 306

Query: 305 RRVDLIVAGALPVGEILSRVTQYGFNISYGYGMTEALGPVIIRPISGGGGGVSLLE---- 364
             V ++  G+ P   ++ +V + GF + + YG+TEA GPV+          +   +    
Sbjct: 307 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 366

Query: 365 ------DLITSLEVDVKDSTSMESVAADGETLGEIMLRGNTLMSGYFENPKATKAAFSGD 424
                  ++   EVDV++  + ESV  DG+T+GEI+++G+++M GY +NPKAT  AF   
Sbjct: 367 KARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKHG 426

Query: 425 WYRTGDVAVRHRSGRIEMKDRANDVIVTADGAAVSTVEVEAVLLSHPEVAEAAVVGKYEA 484
           W  +GDV V H  G +E+KDR+ D+I++  G  +S+VEVE ++  +P+V E AVV     
Sbjct: 427 WLNSGDVGVIHPDGHVEIKDRSKDIIISG-GENISSVEVENIIYKYPKVLETAVVAMPHP 486

Query: 485 GFGEIRPCGFVKLRNG----------CRASAEEIVGFCGDHLPEFMVPASIVFGD-LPLN 525
            +GE  PC FV L  G                +++ +C ++LP FM P  +VF D LP N
Sbjct: 487 TWGE-TPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKN 546