MS020785 (gene) Bitter gourd (TR) v1

Overview
NameMS020785
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionphosphoglucan phosphatase LSF2, chloroplastic
Locationscaffold487: 512852 .. 515882 (+)
RNA-Seq ExpressionMS020785
SyntenyMS020785
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACTTCAAGTTTTCCCCCTTCTTTTTCATCCCTCTCCGTAATTCTTCCCCTTCAGAATCATACACCCAACAAATTTTTATCATTCCCTTCGAAGCTTTCGAAACCCCACTTGCAATTCCACATAATCTGCTGCGGAATGCCTGAGAGTGGGGTTCGGCAAAACCCCACAAGCGGCGGAGCTTCCACTTCCAGGAACAGCACGGAGATGTACAATATGACTATGAAGAAGATGATGAAGAACCCTTATGAGTATCACCATGAACTGGGTCAGTTTCTTCTTTTCTTCATCCTGTTTTTCGATTTTTCCTTTTGATCCTTGAATTCTGTAATTCACGGCCAGCCATTTTATATGCTTCGATCGCTTTCCTTCTGCAATTTGTTAATGGGTGATTTTGGAGCATTTGTGTTGTTTGGAATGAGAAGTAATTGGGCGATAGGATTGATATTGGGACTTTAGAAGTTCATGAATAGAAGTTTTTTTTTTTTTTTTTTTTCTGTTTCCTAGATATTGAGCCTTCTCTGTGGATTTCTAATGATTTATGATAGCTTTTCGCAAAGTTACCCAAAGTTAATGGTGCTTTGAGTAATTAGAGGGCAAAGACATTTTGTAACCTCTTTGAGTTCTTTGAGAAGTCCATAAAATTGTGGGCTCTCTCTTTGAGTTTCAGTTTACTAGATTTGATTTTAGGAGCAGTTTTACTGGTTACTTAGGTTCTGTTTGGTTCATGATTCAAATAGAAGGGATTAAATAGATAAGAGAGAAGAAGCTATTAGAGAGAGAGACTCTGCTTTTGAGAAAATACAGGGTTCTACTTTTCAGGAAATCTCTTTCTGTATTCATTTGAAAACAAAGAGAACATTGAAAGAATGATTTCAAAAGTTGGCTTAAATGGGTAATTGTCTTTGAAAATCATTTTCGAAAACACAAGTACCAAATGCAATTCAGTGTATCCTTGGCATCCCTTGTTTTCAAACACGTAAAAGGATAGCGGGTTTGATACCAAATCGGTATGAAGTAACTTTTCCAGTTAGAATTCTGATAGACAAAACTTGAAGTAATATCAGTTTGCTCATAATGCTCCTGCAATCAGGTATGAACTATACTGTGATAGCTGACAATTTGATTGTCGGTTCCCAGCCTCAAAAACCCGAAGATATCGATTTCTTGAAGGAGGAAGAAGGTGTTGCCTATATTCTGAATTTACAGCAGGACAAGGATGTTGAGTATTGGGGAGTCGACTTACGATCAATTATAGAAAGATGTAAAGAACTTGGAATTCATCACATGAGGAAACCGGTAAGCACATATACTTTTCATACCCTGTGTCTTAAATCTCTGGTAGACCCATTTTCAAATATATACTGATTTCTCTCCCTTTTTAATCTTGAACTGCTGGAAACTGATTTCTAACTGTTCTATGTTGTATGTACTTTCATCAAATGGAAATATGAAATCTGGGTATGGGAAAAGCTTTTTCTTAAGCAGGAAATGTTCTTCACTCAAGTTTATTTTCAGCTTATCTGTGCATTAAGAAAGTAAATTAAAAAAAGGAAAGCATGGTTTCTCATAAAAATAATAATAATAAATAAAAGTTTATATACTATTTTGGTTGCTATACTTTTAGCTCTTGTTTATTTTGATCCATGTAATTTCAAAACATAGATCTTGGTCCATGTACTTTCATATTTTATTATTTTATCCATCTTAGTCCTTATACTTTCTGAATGTCTACTTTTAAAAAATGATTGTTTTGGTCCATTCATTTTCCTTTTTGTCTCATTTTTTTCCTATCACAATTTCAACGTGACGATATACTCAATAAATTTCTTGCAATGTACTCATAACTTTGTATTAAAGGGTTTTAATTTGTGTTAAAATGTTGCAAACAAAGACCAAAGTAGTCACTTTTTTTCTCACAAAAAAAAGTAGCGACATTTTAAAACTACAAGGCTAAATTGGATGTTTTGAGTGTAAAAAACTAAAATGAACCAAAGTTGAACGTATAGGACCCAAATGAACATTTTTAAAGTATAAGGACTTGAATGAACTGAGCATGAATTTCCAGGGACTAAAATAGTATTTAAACTACAAATAAATAAATAATTAATTAAATAAATTTAGCGTAACTCATTTGCTTAAGTTTTTGGGTTCAAGGGTGATTTAACATATTATTATAGTAGTCTAGTATGAGGTCCTTTATTCGAATTTCAATAATATAATTTTCTTCTTATTTAATATTGATAATCACTCGTTGGGCTTTGTGTAAATTTCTGAGCCACAAGTAAGGAGGAGTATTAGAGTTTGAAATTAGCTTAAGCTTTTGGATTCAACGATTATTTAGCACTACGAAACAAACGAAACAAGTGAGAGACTAGTATCTCTTGTAGCAACTTGCACTAACGTCGTGAATTTGTCTGCTCTGTTTGTTTTTGGTGGCACGTCGGGTTAACCCATTCTTTTGTAGGCAAGAGATTTTGATCCAGATTCCATGAGAAATGGATTACCTAAAGCTGTTTCCTTGCTAGAATGGGCGATCTCGGAGGGTAAAGGAAAAGTTTATGTCCATTGCACCGCTGGACTGGGAAGGGCTCCTGCCGTTGCAATAGCTTACTTGTTCTGGTTTTGTGGAATGAATGTGAGTTCTTCTTTTACTTAGAAATTAATGCAGTACATTAATAATGCCTGCTCTTGACATGTTTTGAGCTGGAAAGTGTAAAATATGACAGTAGATATCAATGTTGACATTTTCTGATTTACACAGACTTTTTTACTCCTCAAAATAAAACTACCAAGTTATTGGTTTCTTATTTTGGCTATGTGACAGTTAAATACCGCATATGAAGCACTGACCTCGAAGCGACCTTGTGGACCGAGTAAAAGAGCGATTCGTGGAGCCACATACGATCTATGTAAAACAGATCAATGGAAGGAGCCATTCGAGAGCCTTCCCGACAATGCATTTGAAGGTGTTGCAGATTGGGAGAGAAACTTGATTCAGGATCGGGTCCGCTCTCTCCGCAAAACT

mRNA sequence

ATGGCAACTTCAAGTTTTCCCCCTTCTTTTTCATCCCTCTCCGTAATTCTTCCCCTTCAGAATCATACACCCAACAAATTTTTATCATTCCCTTCGAAGCTTTCGAAACCCCACTTGCAATTCCACATAATCTGCTGCGGAATGCCTGAGAGTGGGGTTCGGCAAAACCCCACAAGCGGCGGAGCTTCCACTTCCAGGAACAGCACGGAGATGTACAATATGACTATGAAGAAGATGATGAAGAACCCTTATGAGTATCACCATGAACTGGGTATGAACTATACTGTGATAGCTGACAATTTGATTGTCGGTTCCCAGCCTCAAAAACCCGAAGATATCGATTTCTTGAAGGAGGAAGAAGGTGTTGCCTATATTCTGAATTTACAGCAGGACAAGGATGTTGAGTATTGGGGAGTCGACTTACGATCAATTATAGAAAGATGTAAAGAACTTGGAATTCATCACATGAGGAAACCGGCAAGAGATTTTGATCCAGATTCCATGAGAAATGGATTACCTAAAGCTGTTTCCTTGCTAGAATGGGCGATCTCGGAGGGTAAAGGAAAAGTTTATGTCCATTGCACCGCTGGACTGGGAAGGGCTCCTGCCGTTGCAATAGCTTACTTGTTCTGGTTTTGTGGAATGAATTTAAATACCGCATATGAAGCACTGACCTCGAAGCGACCTTGTGGACCGAGTAAAAGAGCGATTCGTGGAGCCACATACGATCTATGTAAAACAGATCAATGGAAGGAGCCATTCGAGAGCCTTCCCGACAATGCATTTGAAGGTGTTGCAGATTGGGAGAGAAACTTGATTCAGGATCGGGTCCGCTCTCTCCGCAAAACT

Coding sequence (CDS)

ATGGCAACTTCAAGTTTTCCCCCTTCTTTTTCATCCCTCTCCGTAATTCTTCCCCTTCAGAATCATACACCCAACAAATTTTTATCATTCCCTTCGAAGCTTTCGAAACCCCACTTGCAATTCCACATAATCTGCTGCGGAATGCCTGAGAGTGGGGTTCGGCAAAACCCCACAAGCGGCGGAGCTTCCACTTCCAGGAACAGCACGGAGATGTACAATATGACTATGAAGAAGATGATGAAGAACCCTTATGAGTATCACCATGAACTGGGTATGAACTATACTGTGATAGCTGACAATTTGATTGTCGGTTCCCAGCCTCAAAAACCCGAAGATATCGATTTCTTGAAGGAGGAAGAAGGTGTTGCCTATATTCTGAATTTACAGCAGGACAAGGATGTTGAGTATTGGGGAGTCGACTTACGATCAATTATAGAAAGATGTAAAGAACTTGGAATTCATCACATGAGGAAACCGGCAAGAGATTTTGATCCAGATTCCATGAGAAATGGATTACCTAAAGCTGTTTCCTTGCTAGAATGGGCGATCTCGGAGGGTAAAGGAAAAGTTTATGTCCATTGCACCGCTGGACTGGGAAGGGCTCCTGCCGTTGCAATAGCTTACTTGTTCTGGTTTTGTGGAATGAATTTAAATACCGCATATGAAGCACTGACCTCGAAGCGACCTTGTGGACCGAGTAAAAGAGCGATTCGTGGAGCCACATACGATCTATGTAAAACAGATCAATGGAAGGAGCCATTCGAGAGCCTTCCCGACAATGCATTTGAAGGTGTTGCAGATTGGGAGAGAAACTTGATTCAGGATCGGGTCCGCTCTCTCCGCAAAACT

Protein sequence

MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT
Homology
BLAST of MS020785 vs. NCBI nr
Match: XP_022146766.1 (phosphoglucan phosphatase LSF2, chloroplastic [Momordica charantia] >XP_022146767.1 phosphoglucan phosphatase LSF2, chloroplastic [Momordica charantia])

HSP 1 Score: 585.9 bits (1509), Expect = 1.9e-163
Identity = 280/283 (98.94%), Postives = 282/283 (99.65%), Query Frame = 0

Query: 1   MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSG 60
           MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFH ICCGMPE+GVRQNPTSG
Sbjct: 1   MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSG 60

Query: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 120
           GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKP+DIDFLKEEE
Sbjct: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE 120

Query: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE 180
           GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE
Sbjct: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE 180

Query: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 240
           WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA
Sbjct: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 240

Query: 241 TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 284
           TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT
Sbjct: 241 TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 283

BLAST of MS020785 vs. NCBI nr
Match: XP_011655031.1 (phosphoglucan phosphatase LSF2, chloroplastic [Cucumis sativus] >XP_011655032.1 phosphoglucan phosphatase LSF2, chloroplastic [Cucumis sativus] >KGN50717.1 hypothetical protein Csa_005846 [Cucumis sativus])

HSP 1 Score: 488.4 bits (1256), Expect = 4.1e-134
Identity = 228/276 (82.61%), Postives = 253/276 (91.67%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNKFL F S LSKP  +F+ ICCG+PESG++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKFLPFSSILSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILN 127
             E YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKPEDI+FLKEEEGVAYILN
Sbjct: 67  RMEDYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG+DL+SIIERCKELGIHHMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGIDLQSIIERCKELGIHHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 284
           D WKEPFESLPDNAFE +ADWERNLI+DRVR+LR+T
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIRDRVRALRRT 282

BLAST of MS020785 vs. NCBI nr
Match: XP_038891555.1 (phosphoglucan phosphatase LSF2, chloroplastic [Benincasa hispida])

HSP 1 Score: 485.7 bits (1249), Expect = 2.7e-133
Identity = 230/283 (81.27%), Postives = 253/283 (89.40%), Query Frame = 0

Query: 1   MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSG 60
           MA SS  P FS L    P QN+ PNKFL F S +SKPH + + ICCG PESG++Q P++ 
Sbjct: 44  MAASSL-PCFSLLFAFFPHQNNAPNKFLPFSSIISKPHFRLNRICCGAPESGIQQIPSTS 103

Query: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 120
            AS+S+N  E YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKPEDI+FLKEEE
Sbjct: 104 RASSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEE 163

Query: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE 180
           GVAYILNLQQDKDVEYWG+DL+SII+RCKELGIHHMR+PA+DFDPDS+RNGLPKAVSLLE
Sbjct: 164 GVAYILNLQQDKDVEYWGIDLQSIIKRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLE 223

Query: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 240
           WAISEGKGKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGA
Sbjct: 224 WAISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 283

Query: 241 TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 284
           TYDL K D WKEPFESLPDNAFE +ADWERNLI+DRV SLR+T
Sbjct: 284 TYDLSKNDPWKEPFESLPDNAFEDIADWERNLIRDRVHSLRRT 325

BLAST of MS020785 vs. NCBI nr
Match: XP_008456918.1 (PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X3 [Cucumis melo] >XP_008456919.1 PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X3 [Cucumis melo])

HSP 1 Score: 481.1 bits (1237), Expect = 6.6e-132
Identity = 225/276 (81.52%), Postives = 250/276 (90.58%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNK L F S LSKP  +F+ ICCG+PESG++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILN 127
             + YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKPEDI+FLKEEEGVAYILN
Sbjct: 67  RMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG++L+SIIERCKELGI HMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 284
           D WKEPFESLPDNAFE +ADWERNLI DRVRSLR+T
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIWDRVRSLRRT 282

BLAST of MS020785 vs. NCBI nr
Match: XP_022942267.1 (phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 473.8 bits (1218), Expect = 1.0e-129
Identity = 224/274 (81.75%), Postives = 245/274 (89.42%), Query Frame = 0

Query: 10  FSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRNST 69
           FSSL  + P  N+  NK L F S  SKP  + + I CG+PESG+RQNP+S  AS+S+N  
Sbjct: 9   FSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSSRASSSKNRM 68

Query: 70  EMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQ 129
           E YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQ
Sbjct: 69  EEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQ 128

Query: 130 QDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGK 189
           QDKDVEYWG+DL+S+IERCKELGIHHMR+PA+DFDPDS+RNGLPKAVSLLEWAISEGKGK
Sbjct: 129 QDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGK 188

Query: 190 VYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQ 249
            YVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSK+AIRGATYDL K D 
Sbjct: 189 AYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGATYDLSKNDP 248

Query: 250 WKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 284
            KEPFESLPDNAFE +ADWERNLIQDRVRSLR+T
Sbjct: 249 GKEPFESLPDNAFEDIADWERNLIQDRVRSLRRT 282

BLAST of MS020785 vs. ExPASy Swiss-Prot
Match: Q9SRK5 (Phosphoglucan phosphatase LSF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LSF2 PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.5e-102
Identity = 177/265 (66.79%), Postives = 213/265 (80.38%), Query Frame = 0

Query: 20  QNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGAS-TSRNSTEMYNMTMKK 79
           +N   + F S  +K S   L+F     G+      +NP + G S +S+N  E YN  MK+
Sbjct: 19  ENEKSSCFTSI-NKKSSLDLRFPRNLAGVSCKFSGENPGTNGVSLSSKNKMEDYNTAMKR 78

Query: 80  MMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQDKDVEYWG 139
           +M++PYEYHH+LGMNYT+I D LIVGSQPQKPEDID LK+E+ VAYILNLQQDKD+EYWG
Sbjct: 79  LMRSPYEYHHDLGMNYTLIRDELIVGSQPQKPEDIDHLKQEQNVAYILNLQQDKDIEYWG 138

Query: 140 VDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGL 199
           +DL SI+ RCKELGI HMR+PA+DFDP S+R+ LPKAVS LEWA+SEGKG+VYVHC+AGL
Sbjct: 139 IDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLPKAVSSLEWAVSEGKGRVYVHCSAGL 198

Query: 200 GRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQWKEPFESLP 259
           GRAP V+IAY++WFC MNLNTAY+ L SKRPCGP+K AIRGATYDL K D WKEPFESLP
Sbjct: 199 GRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPNKGAIRGATYDLAKNDPWKEPFESLP 258

Query: 260 DNAFEGVADWERNLIQDRVRSLRKT 284
           +NAFE +ADWER LIQ+RVR+LR T
Sbjct: 259 ENAFEDIADWERKLIQERVRALRGT 282

BLAST of MS020785 vs. ExPASy Swiss-Prot
Match: Q9FEB5 (Phosphoglucan phosphatase DSP4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DSP4 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.3e-37
Identity = 97/239 (40.59%), Postives = 129/239 (53.97%), Query Frame = 0

Query: 28  LSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRNSTEMYNMTMKKMMKNPYEYH 87
           +S P K + P  +  ++   + ES  + +    G +     ++ Y+  M + M     Y 
Sbjct: 36  ISPPIKANDP--KSRLVLHAVSES--KSSSEMSGVAKDEEKSDEYSQDMTQAMGAVLTYR 95

Query: 88  HELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQDKDVEYWGVDLRSIIER 147
           HELGMNY  I  +LIVGS  Q PED+D L+ + GV  I  LQQD D+EY+GVD+ SI   
Sbjct: 96  HELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KIGVKTIFCLQQDPDLEYFGVDISSIQAY 155

Query: 148 CKELG-IHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGRAPAVAI 207
            K+   I H+R   RDFD   +R  LP  V  L  A+    G  YVHCTAG+GRAPAVA+
Sbjct: 156 AKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTLYKAVKRNGGVTYVHCTAGMGRAPAVAL 215

Query: 208 AYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQWKEPFESLPDNAFEGV 266
            Y+FW  G  L  A++ L SKR C P   AIR AT D+    + K    +L D  F  V
Sbjct: 216 TYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRNATIDILTGLKRKTVTLTLKDKGFSRV 269

BLAST of MS020785 vs. ExPASy Swiss-Prot
Match: G4LTX4 (Phosphoglucan phosphatase DSP4, amyloplastic OS=Castanea sativa OX=21020 GN=DSP4 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.1e-36
Identity = 92/211 (43.60%), Postives = 114/211 (54.03%), Query Frame = 0

Query: 51  SGVRQNPTSGGASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKP 110
           SG   +  +  A+     +E Y+  M + M     Y HELG+NY  I  +LIVGS  Q P
Sbjct: 50  SGPTSSAETSDANVEEEKSETYSTNMTEAMGAVLTYRHELGINYNFIRPDLIVGSCLQTP 109

Query: 111 EDIDFLKEEEGVAYILNLQQDKDVEYWGVDLRSIIERCKEL-GIHHMRKPARDFDPDSMR 170
           ED+D L+   GV  I  LQQ+ D+EY+GVD+  I E  K    I H+R   RDFD   +R
Sbjct: 110 EDVDKLR-SIGVKTIFCLQQNSDLEYFGVDINGIREYAKTYDDIQHLRAEIRDFDAFDLR 169

Query: 171 NGLPKAVSLLEWAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRP 230
             LP  VS L  AI+   G  YVHCTAGLGRAPAV +AY+FW  G  L  A+  L SKR 
Sbjct: 170 VRLPAVVSKLYKAINRNGGVTYVHCTAGLGRAPAVTLAYMFWVQGYKLIEAHNLLQSKRS 229

Query: 231 CGPSKRAIRGATYDLCK-------TDQWKEP 254
           C P   AI+ A  D+         T  WK P
Sbjct: 230 CFPKLDAIKSAAADILTGLRKKVVTLTWKNP 259

BLAST of MS020785 vs. ExPASy Swiss-Prot
Match: F4J117 (Phosphoglucan phosphatase LSF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LSF1 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 8.1e-24
Identity = 60/164 (36.59%), Postives = 88/164 (53.66%), Query Frame = 0

Query: 81  KNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQDKDVEYWGVD 140
           K    Y+H LGM Y+ I + + VGS  Q  ED++ L  E G+  ILN Q   + + WG+D
Sbjct: 279 KGELSYNHALGMRYSKITEQIYVGSCIQTEEDVENL-SEAGITAILNFQGGTEAQNWGID 338

Query: 141 LRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGR 200
            +SI + C++  +  +  P +D D   +R  LP  V LL   + +   +V+V CT G  R
Sbjct: 339 SQSINDACQKSEVLMINYPIKDADSFDLRKKLPLCVGLLLRLLKKNH-RVFVTCTTGFDR 398

Query: 201 APAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL 245
           + A  IAYL W    +L+ AY  +T    C P + AI  AT+DL
Sbjct: 399 SSACVIAYLHWMTDTSLHAAYSFVTGLHACKPDRPAIAWATWDL 440

BLAST of MS020785 vs. ExPASy Swiss-Prot
Match: Q1M199 (Laforin OS=Canis lupus familiaris OX=9615 GN=EPM2A PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.2e-11
Identity = 50/164 (30.49%), Postives = 80/164 (48.78%), Query Frame = 0

Query: 80  MKNPYEYHHEL----GMNYTVIADNLIVGSQPQKPEDIDF-LKEEEGVAYILNLQQDKDV 139
           MK+  +++  +     M+Y+ I  N+ +GS P++ E I   LK E G+  ++N Q + D+
Sbjct: 139 MKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQVEHITIKLKHELGITAVMNFQTEWDI 198

Query: 140 EYWGVDLR---------SIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISE 199
                            ++I+  KE G+ ++  P  D   +     LP+AV LL  A+ E
Sbjct: 199 VQNSSGCNRYPEPMTPDTMIKLYKEEGLVYIWMPTPDMSTEGRVQMLPQAVCLLH-ALLE 258

Query: 200 GKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRP 230
               VYVHC AG+GR+ A    +L +  G NL      L +KRP
Sbjct: 259 NGHTVYVHCNAGVGRSTAAVCGWLQYVMGWNLRKVQYFLMAKRP 301

BLAST of MS020785 vs. ExPASy TrEMBL
Match: A0A6J1D0C4 (phosphoglucan phosphatase LSF2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015894 PE=4 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 9.2e-164
Identity = 280/283 (98.94%), Postives = 282/283 (99.65%), Query Frame = 0

Query: 1   MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSG 60
           MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFH ICCGMPE+GVRQNPTSG
Sbjct: 1   MATSSFPPSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHRICCGMPETGVRQNPTSG 60

Query: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEE 120
           GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKP+DIDFLKEEE
Sbjct: 61  GASTSRNSTEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPKDIDFLKEEE 120

Query: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE 180
           GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE
Sbjct: 121 GVAYILNLQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLE 180

Query: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 240
           WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA
Sbjct: 181 WAISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGA 240

Query: 241 TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 284
           TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT
Sbjct: 241 TYDLCKTDQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 283

BLAST of MS020785 vs. ExPASy TrEMBL
Match: A0A0A0KQM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G218830 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.0e-134
Identity = 228/276 (82.61%), Postives = 253/276 (91.67%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNKFL F S LSKP  +F+ ICCG+PESG++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKFLPFSSILSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILN 127
             E YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKPEDI+FLKEEEGVAYILN
Sbjct: 67  RMEDYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG+DL+SIIERCKELGIHHMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGIDLQSIIERCKELGIHHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 284
           D WKEPFESLPDNAFE +ADWERNLI+DRVR+LR+T
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIRDRVRALRRT 282

BLAST of MS020785 vs. ExPASy TrEMBL
Match: A0A1S3C4E0 (phosphoglucan phosphatase LSF2, chloroplastic isoform X3 OS=Cucumis melo OX=3656 GN=LOC103496723 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.2e-132
Identity = 225/276 (81.52%), Postives = 250/276 (90.58%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNK L F S LSKP  +F+ ICCG+PESG++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILN 127
             + YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKPEDI+FLKEEEGVAYILN
Sbjct: 67  RMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG++L+SIIERCKELGI HMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 284
           D WKEPFESLPDNAFE +ADWERNLI DRVRSLR+T
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIWDRVRSLRRT 282

BLAST of MS020785 vs. ExPASy TrEMBL
Match: A0A6J1FPR7 (phosphoglucan phosphatase LSF2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111447363 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 5.1e-130
Identity = 224/274 (81.75%), Postives = 245/274 (89.42%), Query Frame = 0

Query: 10  FSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRNST 69
           FSSL  + P  N+  NK L F S  SKP  + + I CG+PESG+RQNP+S  AS+S+N  
Sbjct: 9   FSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSSRASSSKNRM 68

Query: 70  EMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQ 129
           E YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQ
Sbjct: 69  EEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQ 128

Query: 130 QDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGK 189
           QDKDVEYWG+DL+S+IERCKELGIHHMR+PA+DFDPDS+RNGLPKAVSLLEWAISEGKGK
Sbjct: 129 QDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGK 188

Query: 190 VYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQ 249
            YVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSK+AIRGATYDL K D 
Sbjct: 189 AYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGATYDLSKNDP 248

Query: 250 WKEPFESLPDNAFEGVADWERNLIQDRVRSLRKT 284
            KEPFESLPDNAFE +ADWERNLIQDRVRSLR+T
Sbjct: 249 GKEPFESLPDNAFEDIADWERNLIQDRVRSLRRT 282

BLAST of MS020785 vs. ExPASy TrEMBL
Match: A0A1S3C3W8 (phosphoglucan phosphatase LSF2, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496723 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 2.5e-129
Identity = 220/271 (81.18%), Postives = 245/271 (90.41%), Query Frame = 0

Query: 8   PSFSSLSVILPLQNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRN 67
           P FSSL  + PLQN+ PNK L F S LSKP  +F+ ICCG+PESG++QNP++   S+S+N
Sbjct: 7   PCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSRPSSSKN 66

Query: 68  STEMYNMTMKKMMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILN 127
             + YN+ MKKMM+NPYEYHHELGMNYTVIADNLIVGSQPQKPEDI+FLKEEEGVAYILN
Sbjct: 67  RMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEGVAYILN 126

Query: 128 LQQDKDVEYWGVDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGK 187
           LQQDKDVEYWG++L+SIIERCKELGI HMR+PA+DFDPDS+R GLPKAVSLLEWAIS+GK
Sbjct: 127 LQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEWAISKGK 186

Query: 188 GKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKT 247
           GKVYVHCTAGLGRAPAVAIAYL+WFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL K 
Sbjct: 187 GKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLSKN 246

Query: 248 DQWKEPFESLPDNAFEGVADWERNLIQDRVR 279
           D WKEPFESLPDNAFE +ADWERNLI DRV+
Sbjct: 247 DPWKEPFESLPDNAFEDIADWERNLIWDRVQ 277

BLAST of MS020785 vs. TAIR 10
Match: AT3G10940.1 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 374.0 bits (959), Expect = 1.1e-103
Identity = 177/265 (66.79%), Postives = 213/265 (80.38%), Query Frame = 0

Query: 20  QNHTPNKFLSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGAS-TSRNSTEMYNMTMKK 79
           +N   + F S  +K S   L+F     G+      +NP + G S +S+N  E YN  MK+
Sbjct: 19  ENEKSSCFTSI-NKKSSLDLRFPRNLAGVSCKFSGENPGTNGVSLSSKNKMEDYNTAMKR 78

Query: 80  MMKNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQDKDVEYWG 139
           +M++PYEYHH+LGMNYT+I D LIVGSQPQKPEDID LK+E+ VAYILNLQQDKD+EYWG
Sbjct: 79  LMRSPYEYHHDLGMNYTLIRDELIVGSQPQKPEDIDHLKQEQNVAYILNLQQDKDIEYWG 138

Query: 140 VDLRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGL 199
           +DL SI+ RCKELGI HMR+PA+DFDP S+R+ LPKAVS LEWA+SEGKG+VYVHC+AGL
Sbjct: 139 IDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLPKAVSSLEWAVSEGKGRVYVHCSAGL 198

Query: 200 GRAPAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQWKEPFESLP 259
           GRAP V+IAY++WFC MNLNTAY+ L SKRPCGP+K AIRGATYDL K D WKEPFESLP
Sbjct: 199 GRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPNKGAIRGATYDLAKNDPWKEPFESLP 258

Query: 260 DNAFEGVADWERNLIQDRVRSLRKT 284
           +NAFE +ADWER LIQ+RVR+LR T
Sbjct: 259 ENAFEDIADWERKLIQERVRALRGT 282

BLAST of MS020785 vs. TAIR 10
Match: AT3G52180.1 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 158.3 bits (399), Expect = 9.1e-39
Identity = 97/239 (40.59%), Postives = 129/239 (53.97%), Query Frame = 0

Query: 28  LSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRNSTEMYNMTMKKMMKNPYEYH 87
           +S P K + P  +  ++   + ES  + +    G +     ++ Y+  M + M     Y 
Sbjct: 36  ISPPIKANDP--KSRLVLHAVSES--KSSSEMSGVAKDEEKSDEYSQDMTQAMGAVLTYR 95

Query: 88  HELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQDKDVEYWGVDLRSIIER 147
           HELGMNY  I  +LIVGS  Q PED+D L+ + GV  I  LQQD D+EY+GVD+ SI   
Sbjct: 96  HELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KIGVKTIFCLQQDPDLEYFGVDISSIQAY 155

Query: 148 CKELG-IHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGRAPAVAI 207
            K+   I H+R   RDFD   +R  LP  V  L  A+    G  YVHCTAG+GRAPAVA+
Sbjct: 156 AKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTLYKAVKRNGGVTYVHCTAGMGRAPAVAL 215

Query: 208 AYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQWKEPFESLPDNAFEGV 266
            Y+FW  G  L  A++ L SKR C P   AIR AT D+    + K    +L D  F  V
Sbjct: 216 TYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRNATIDILTGLKRKTVTLTLKDKGFSRV 269

BLAST of MS020785 vs. TAIR 10
Match: AT3G52180.2 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 158.3 bits (399), Expect = 9.1e-39
Identity = 97/239 (40.59%), Postives = 129/239 (53.97%), Query Frame = 0

Query: 28  LSFPSKLSKPHLQFHIICCGMPESGVRQNPTSGGASTSRNSTEMYNMTMKKMMKNPYEYH 87
           +S P K + P  +  ++   + ES  + +    G +     ++ Y+  M + M     Y 
Sbjct: 36  ISPPIKANDP--KSRLVLHAVSES--KSSSEMSGVAKDEEKSDEYSQDMTQAMGAVLTYR 95

Query: 88  HELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQDKDVEYWGVDLRSIIER 147
           HELGMNY  I  +LIVGS  Q PED+D L+ + GV  I  LQQD D+EY+GVD+ SI   
Sbjct: 96  HELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KIGVKTIFCLQQDPDLEYFGVDISSIQAY 155

Query: 148 CKELG-IHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGRAPAVAI 207
            K+   I H+R   RDFD   +R  LP  V  L  A+    G  YVHCTAG+GRAPAVA+
Sbjct: 156 AKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTLYKAVKRNGGVTYVHCTAGMGRAPAVAL 215

Query: 208 AYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDLCKTDQWKEPFESLPDNAFEGV 266
            Y+FW  G  L  A++ L SKR C P   AIR AT D+    + K    +L D  F  V
Sbjct: 216 TYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRNATIDILTGLKRKTVTLTLKDKGFSRV 269

BLAST of MS020785 vs. TAIR 10
Match: AT3G01510.1 (like SEX4 1 )

HSP 1 Score: 112.5 bits (280), Expect = 5.7e-25
Identity = 60/164 (36.59%), Postives = 88/164 (53.66%), Query Frame = 0

Query: 81  KNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQDKDVEYWGVD 140
           K    Y+H LGM Y+ I + + VGS  Q  ED++ L  E G+  ILN Q   + + WG+D
Sbjct: 279 KGELSYNHALGMRYSKITEQIYVGSCIQTEEDVENL-SEAGITAILNFQGGTEAQNWGID 338

Query: 141 LRSIIERCKELGIHHMRKPARDFDPDSMRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGR 200
            +SI + C++  +  +  P +D D   +R  LP  V LL   + +   +V+V CT G  R
Sbjct: 339 SQSINDACQKSEVLMINYPIKDADSFDLRKKLPLCVGLLLRLLKKNH-RVFVTCTTGFDR 398

Query: 201 APAVAIAYLFWFCGMNLNTAYEALTSKRPCGPSKRAIRGATYDL 245
           + A  IAYL W    +L+ AY  +T    C P + AI  AT+DL
Sbjct: 399 SSACVIAYLHWMTDTSLHAAYSFVTGLHACKPDRPAIAWATWDL 440

BLAST of MS020785 vs. TAIR 10
Match: AT3G55270.1 (mitogen-activated protein kinase phosphatase 1 )

HSP 1 Score: 42.4 bits (98), Expect = 7.3e-04
Identity = 16/46 (34.78%), Postives = 29/46 (63.04%), Query Frame = 0

Query: 183 ISEGKGKVYVHCTAGLGRAPAVAIAYLFWFCGMNLNTAYEALTSKR 229
           + E  G+++VHC  G+ R+ ++ IAYL W  G + + A++ + S R
Sbjct: 224 VREQSGRIFVHCCQGVSRSTSLVIAYLMWREGQSFDDAFQYVKSAR 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146766.11.9e-16398.94phosphoglucan phosphatase LSF2, chloroplastic [Momordica charantia] >XP_02214676... [more]
XP_011655031.14.1e-13482.61phosphoglucan phosphatase LSF2, chloroplastic [Cucumis sativus] >XP_011655032.1 ... [more]
XP_038891555.12.7e-13381.27phosphoglucan phosphatase LSF2, chloroplastic [Benincasa hispida][more]
XP_008456918.16.6e-13281.52PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X3 [Cucumis mel... [more]
XP_022942267.11.0e-12981.75phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SRK51.5e-10266.79Phosphoglucan phosphatase LSF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FEB51.3e-3740.59Phosphoglucan phosphatase DSP4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
G4LTX41.1e-3643.60Phosphoglucan phosphatase DSP4, amyloplastic OS=Castanea sativa OX=21020 GN=DSP4... [more]
F4J1178.1e-2436.59Phosphoglucan phosphatase LSF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q1M1991.2e-1130.49Laforin OS=Canis lupus familiaris OX=9615 GN=EPM2A PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D0C49.2e-16498.94phosphoglucan phosphatase LSF2, chloroplastic OS=Momordica charantia OX=3673 GN=... [more]
A0A0A0KQM62.0e-13482.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G218830 PE=4 SV=1[more]
A0A1S3C4E03.2e-13281.52phosphoglucan phosphatase LSF2, chloroplastic isoform X3 OS=Cucumis melo OX=3656... [more]
A0A6J1FPR75.1e-13081.75phosphoglucan phosphatase LSF2, chloroplastic OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A1S3C3W82.5e-12981.18phosphoglucan phosphatase LSF2, chloroplastic isoform X2 OS=Cucumis melo OX=3656... [more]
Match NameE-valueIdentityDescription
AT3G10940.11.1e-10366.79dual specificity protein phosphatase (DsPTP1) family protein [more]
AT3G52180.19.1e-3940.59dual specificity protein phosphatase (DsPTP1) family protein [more]
AT3G52180.29.1e-3940.59dual specificity protein phosphatase (DsPTP1) family protein [more]
AT3G01510.15.7e-2536.59like SEX4 1 [more]
AT3G55270.17.3e-0434.78mitogen-activated protein kinase phosphatase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020422Dual specificity protein phosphatase domainSMARTSM00195dsp_5coord: 93..243
e-value: 1.5E-4
score: 22.6
IPR020422Dual specificity protein phosphatase domainPROSITEPS50054TYR_PHOSPHATASE_DUALcoord: 93..253
score: 28.15156
IPR029021Protein-tyrosine phosphatase-likeGENE3D3.90.190.10Protein tyrosine phosphatase superfamilycoord: 76..283
e-value: 1.7E-68
score: 232.1
IPR029021Protein-tyrosine phosphatase-likeSUPERFAMILY52799(Phosphotyrosine protein) phosphatases IIcoord: 85..233
IPR000340Dual specificity phosphatase, catalytic domainPFAMPF00782DSPccoord: 116..230
e-value: 8.0E-16
score: 58.1
NoneNo IPR availablePANTHERPTHR46642:SF2PHOSPHOGLUCAN PHOSPHATASE LSF2, CHLOROPLASTICcoord: 34..283
NoneNo IPR availablePANTHERPTHR46642DUAL SPECIFICITY PHOSPHATASE, SUBGROUP, CATALYTIC DOMAINcoord: 34..283
NoneNo IPR availableCDDcd14526DSP_laforin-likecoord: 92..239
e-value: 7.77479E-81
score: 238.635
IPR000387Tyrosine specific protein phosphatases domainPROSITEPS50056TYR_PHOSPHATASE_2coord: 170..229
score: 11.5674

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020785.1MS020785.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
biological_process GO:0006470 protein dephosphorylation
molecular_function GO:0016791 phosphatase activity
molecular_function GO:0008138 protein tyrosine/serine/threonine phosphatase activity